#=GF ID NiFe_hyd_3_EhaA
#=GF AC PF17367.6
#=GF DE NiFe-hydrogenase-type-3 Eha complex subunit A
#=GF AU El-Gebali S;0000-0003-1378-5495
#=GF SE PRODOM:PD029748
#=GF GA 25.50 25.50;
#=GF TC 29.80 29.30;
#=GF NC 24.30 22.70;
#=GF BM hmmbuild HMM.ann SEED.ann
#=GF SM hmmsearch -Z 75585367 --cpu 4 -E 1000 HMM pfamseq
#=GF TP Family
#=GF RN [1]
#=GF RM 21559116
#=GF RT More than 200 genes required for methane formation from H(2) and
#=GF RT CO(2) and energy conservation are present in Methanothermobacter
#=GF RT marburgensis and Methanothermobacter thermautotrophicus.
#=GF RA Kaster AK, Goenrich M, Seedorf H, Liesegang H, Wollherr A,
#=GF RA Gottschalk G, Thauer RK;
#=GF RL Archaea. 2011;2011:973848.
#=GF RN [2]
#=GF RM 10491142
#=GF RT Methanobacterium thermoautotrophicum encodes two multisubunit
#=GF RT membrane-bound [NiFe] hydrogenases. Transcription of the operons
#=GF RT and sequence analysis of the deduced proteins.
#=GF RA Tersteegen A, Hedderich R;
#=GF RL Eur J Biochem. 1999;264:930-943.
#=GF RN [3]
#=GF RM 19455212
#=GF RT PIRSF family classification system for protein functional and
#=GF RT evolutionary analysis.
#=GF RA Nikolskaya AN, Arighi CN, Huang H, Barker WC, Wu CH;
#=GF RL Evol Bioinform Online. 2007;2:197-209.
#=GF DR INTERPRO; IPR011306;
#=GF DR SO; 0100021; polypeptide_conserved_region;
#=GF CC Energy-converting [NiFe] hydrogenases are membrane-bound enzymes
#=GF CC with a six-subunit core: the large and small hydrogenase
#=GF CC subunits, plus two hydrophilic proteins and two integral
#=GF CC membrane proteins. Their large and small subunits show little
#=GF CC sequence similarity to other [NiFe] hydrogenases, except for key
#=GF CC conserved residues coordinating the active site and [FeS]
#=GF CC cluster. Energy-converting [NiFe] hydrogenases function as ion
#=GF CC pumps, catalyzing the reduction of ferredoxin with H2 driven by
#=GF CC the proton-motive force or the sodium-ion-motive force [1]. Eha
#=GF CC and Ehb hydrogenases contain extra subunits in addition to those
#=GF CC shared by other energy-converting [NiFe] hydrogenases (or
#=GF CC [NiFe]-hydrogenase-3-type). Eha contains a 6[4Fe-4S]
#=GF CC polyferredoxin, a 10[4F-4S] polyferredoxin, ten other predicted
#=GF CC integral membrane proteins (EhaA, EhaB, EhaC, EhaD, EhaE, EhaF,
#=GF CC EhaG, EhaI, EhaK, EhaL) and four hydrophobic subunits (EhaM,
#=GF CC EhaR, EhS, EhT) [2]. Eha and Ehb catalyse the reduction of
#=GF CC low-potential redox carriers (e.g. ferredoxins or
#=GF CC polyferredoxins), which then might function as electron donors
#=GF CC to oxidoreductases [3]. Based on sequence similarity and genome
#=GF CC context analysis, other organisms such as Methanopyrus kandleri,
#=GF CC Methanocaldococcus jannaschii, and Methanothermobacter
#=GF CC marburgensis also encode Eha-like [NiFe]-hydrogenase-3-type
#=GF CC complexes and have very similar eha operon structure [3]. This
#=GF CC domain family can be found on the small membrane proteins that
#=GF CC are predicted to be the EhaA trans-membrane subunits of
#=GF CC multisubunit membrane-bound [NiFe]-hydrogenase Eha complexes.
#=GF SQ 22
#=GS A0A166AX71_9EURY/1-95 AC A0A166AX71.1
#=GS A0A166EFM1_9EURY/1-98 AC A0A166EFM1.1
#=GS A0A1V6N3S9_METAZ/1-98 AC A0A1V6N3S9.1
#=GS A0A1D3L1W4_9EURY/1-97 AC A0A1D3L1W4.1
#=GS A0A8J8C5Y5_9EURY/2-91 AC A0A8J8C5Y5.1
#=GS A0A328P961_9EURY/2-98 AC A0A328P961.1
#=GS U6EAP6_9EURY/1-90 AC U6EAP6.1
#=GS A6UTX2_META3/3-92 AC A6UTX2.1
#=GS F6BBD5_METIK/1-86 AC F6BBD5.1
#=GS A0A133ULJ6_9EURY/1-94 AC A0A133ULJ6.1
#=GS A0A166EX14_9EURY/1-98 AC A0A166EX14.1
#=GS D7DQW5_METV3/1-78 AC D7DQW5.1
#=GS A0A1D2W626_9EURY/1-93 AC A0A1D2W626.1
#=GS A0A2U1S9P0_9EURY/2-99 AC A0A2U1S9P0.1
#=GS EHAA_METTH/1-94 AC O26484.2
#=GS EHAA_METKA/4-94 AC Q8TY29.1
#=GS D3E401_METRM/2-99 AC D3E401.1
#=GS A0A315XN32_9EURY/21-118 AC A0A315XN32.1
#=GS EHAA_METJA/5-98 AC Q57948.1
#=GS EHAA_METMP/1-83 AC Q6LXA4.1
#=GS F0T712_METLA/1-93 AC F0T712.1
#=GS R9SL30_9EURY/1-98 AC R9SL30.1
A0A166AX71_9EURY/1-95 ...migdw--------TIILSYLISIIVSFVVALIIKLP.LTG.EKPIRFSWVNSATFPTPIIAIGLLA.LCFS.INFYwimnGLALAIIFG.VISPFIVKYLFDYIFPEP.......
A0A166EFM1_9EURY/1-98 ........MIIHVSEIYLFIDYLIAIAISIIIALGLRLP.LLP.NKPIRFSFETSALFPTPVIAIGLLA.ICFS.LNIYwiydGMVLAILLG.GFSALFTKYLFDYVFPKP.......
A0A1V6N3S9_METAZ/1-98 ........MIIHVDEMYLLLNLLITVIVSIVIALGLGLP.LLP.KKPIRFSFDKSAIFPTPIFAIGFLA.ICFS.VNFYwiynGMLLAVFWG.VISGLFVKYAFDYVFPKP.......
A0A1D3L1W4_9EURY/1-97 ........MIIHVNVVYTMMSYILAIISAIIVGLILRMP.LLP.ERPMRQSWTISVIFPTAVLAVGFTA.MVFG.LGYEg.tnGMIIGVIVG.VLTALFSKFFLEKIVPRP.......
A0A8J8C5Y5_9EURY/2-91 .......d-IIMVIFMIDIYCYVISVMVSVVIGLIIKLP.LKL.---DKESFEGNLFFPTIFIALSLTS.IVEFlFGLN....-IIFSLFIG.LISPIFSKYV-NIIFPG-v......
A0A328P961_9EURY/2-98 lnfhlfnm---------ILFSYLIAIVSAIIVASLLGLP.IVA.ERPWRRSWTLTVIFPTPIIAAGLLA.VSLK.LGFRgfynTLDLGLIMG.ILSALLVKYIIENIFPRP.......
U6EAP6_9EURY/1-90 ........MIIHAN----VISYLIALAAALILGIILRLP.LLP.EKPMRQSWTISAVFPTAILAIGFYA.MVYK.LGYQ....GYIVALITG.IITALFCKFILEKVVPVP.......
A6UTX2_META3/3-92 .......d-IMGAPLFNNLIYYGGAIIVSIIIGLLLRLP.LKI.---DIDSFEANVIFPTVFIALGLCA.LVEFlFGLN....-LIMGIIIG.FISALFSKYS-MNIFS--gv.....
F6BBD5_METIK/1-86 ....mvis----------MEYYVISVIASIIFAFMLKMP.IIPkERPIRDSFETSVLFPTPIIALGLTA.IDKI.LFTP....DLMSCVLIG.LISALFSKFS-DKIF---g......
A0A133ULJ6_9EURY/1-94 ..midiqs---------QWVVYSLGVIASLIVSRQGSGR.WIP.--ANKFSFHTSAIFPSPVLTVGTIAgLSEF.LSFQepifRYLLPIIIGtVIGAVITMYS--------rimmspk
A0A166EX14_9EURY/1-98 ........MIIHVNEMYLLINYLISVIISMGIAICLGLP.LVP.EKPRRFSWTKSAVFPTPIIAFGLLA.LCYS.AEFYwiynGLALAIITG.VVSSFFMKYLFNYVFPEP.......
D7DQW5_METV3/1-78 .......m----------IELFVIAVLVSMVVAYLLKLK.ISY.---DMNSFEPTVLFPTPFIAVGLTA.IMHK.FIEL....DVVVVVSIG.IISAIFSKYV-NKIF---.......
A0A1D2W626_9EURY/1-93 ........MIIHVDIV-TVLSYVLAVISAIIVGFILRLP.LLP.ERPMRDSWTISIIFPTIIIALGLSA.MVFE.LGWN....GMIVGIVIG.VLSALISKYLLEKILP--ph.....
A0A2U1S9P0_9EURY/2-99 ......my--MGVDISYFIIDYLIAIFSSIVVALILRLP.LLP.EKPYRYSFNVSALYPTPIIAIGVFS.FFVV.LNYLfaynGMLVALIIG.VCSALFVKYLFFYVFPKP.......
EHAA_METTH/1-94 ........MIIHVT---YLSGYLAAIISSIIVSAILGLP.LTP.ERPARHSWTPSAIFPTPVIALGLTA.ISIK.LGVTg.iyGADLGAVAG.VLSAIMTAYFLEDIFPRP.......
EHAA_METKA/4-94 ...pglva-------------VGVAAAVAFGTALALGLPpIQR.DKPRRKSWEVSAAFPTPVIAAGATV.LVIRvIGYHppipLAIVGAVVG.ALSAAFTAYI-EKVFPRP.......
D3E401_METRM/2-99 .......i-SIGANGFQLLINYIVAIVVAVIIAFALRLP.LLP.ERPIRFSWTTSALFPTPIFAIGILA.IFYS.LNVYwiydGLILSVIVG.LASALFVKYGFDYIFPKP.......
A0A315XN32_9EURY/21-118 ......sn--ISIGAFDIVLAYIVAIVCAIAVALVLKLP.LLP.SKPYRYSFDVSALYPTPIIAIGILS.LFLV.LDYTfmynGLVLAVVVG.VLSALFVKYLFDFVFPKP.......
EHAA_METJA/5-98 .......n-SLLVRVMDIIILYLIALITSVIVALVLKLP.IIPkEKPIRFSFETSIIFPTPILALGIEA.IFRNlFGDY....-ISLAFFAG.LFGALLSKYA-DKLFGEP.......
EHAA_METMP/1-83 ......mv---------LLINYAVSLVSAIVVGAVLGMK.LSF.---DMDSFEGSVLFPTPFVAIGLTA.LIGY.LITL....DLVSSIIIG.IFASVFSKFT-NKIFPG-v......
F0T712_METLA/1-93 ........MIIHANVI-LISSYAVAVISSIVVGLLLRIP.LLP.ERPMRQSWTISIVFPTAVLALGFSA.MVFK.LGVD....GLLVAAIIG.IITALFSKYILERVLPEP.......
R9SL30_9EURY/1-98 ........MIYLISETSLLLNYLITIIVSIAVALLLGMP.FLP.EKPRRFSWTKSAVFPTAIIAMGILS.FCFS.INFYwlydGKLLAIIIG.VLSAVFVKYLFRYVFPD-s......
#=GC seq_cons ..........hhh....hllsYllAllsSlllullL+LP.LlP.-+PhR.SapsSslFPTPlIAlGlhA.lshp.lshh....shllulllG.llSAlFsKYhh-hlFPcP.......
//
