GenomeNet

Database: Pfam
Entry: RE_ApaLI
LinkDB: RE_ApaLI
Original site: RE_ApaLI 
#=GF ID   RE_ApaLI
#=GF AC   PF09499.8
#=GF DE   ApaLI-like restriction endonuclease
#=GF AU   Bateman A
#=GF SE   Bateman A
#=GF GA   25.00 25.00;
#=GF TC   61.40 61.20;
#=GF NC   23.00 22.70;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch -Z 17690987 -E 1000 --cpu 4 HMM pfamseq
#=GF TP   Family
#=GF RN   [1]
#=GF RM   9862476
#=GF RT   Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI,
#=GF RT   and SapI restriction-modification systems in Escherichia coli. 
#=GF RA   Xu SY, Xiao JP, Ettwiller L, Holden M, Aliotta J, Poh CL, Dalton
#=GF RA   M, Robinson DP, Petronzio TR, Moran L, Ganatra M, Ware J, Slatko
#=GF RA   B, Benner J; 
#=GF RL   Mol Gen Genet. 1998;260:226-231.
#=GF RN   [2]
#=GF RM   22638584
#=GF RT   Sequence, structure and functional diversity of PD-(D/E)XK
#=GF RT   phosphodiesterase superfamily.
#=GF RA   Steczkiewicz K, Muszewska A, Knizewski L, Rychlewski L, Ginalski
#=GF RA   K;
#=GF RL   Nucleic Acids Res. 2012;40:7016-7045.
#=GF DR   INTERPRO; IPR019036;
#=GF CC   This family includes R.ApaLI and R.XbaI restriction
#=GF CC   endonucleases. ApaLI recognises and cleaves the sequence GTGCAC.
#=GF SQ   16
#=GS E7AAF6_HELFC/1-88       AC E7AAF6.1
#=GS A9WD03_CHLAA/3-191      AC A9WD03.1
#=GS Q5WJU5_BACSK/3-193      AC Q5WJU5.1
#=GS A1ZH81_9BACT/56-244     AC A1ZH81.1
#=GS E7AAF7_HELFC/2-58       AC E7AAF7.1
#=GS A0A0A1H260_9LACO/1-165  AC A0A0A1H260.1
#=GS Q8DP93_STRR6/1-60       AC Q8DP93.1
#=GS K9Z263_CYAAP/1-43       AC K9Z263.1
#=GS A7C3X0_9GAMM/2-70       AC A7C3X0.1
#=GS G2Z232_FLABF/4-191      AC G2Z232.1
#=GS Q8DP92_STRR6/1-43       AC Q8DP92.1
#=GS A7C3W9_9GAMM/1-87       AC A7C3W9.1
#=GS Q6MU56_MYCMS/9-197      AC Q6MU56.1
#=GS D1AUX4_STRM9/6-194      AC D1AUX4.1
#=GS A0A073CCY2_PLAAG/8-119  AC A0A073CCY2.1
#=GS A0A0G1JK17_9BACT/7-196  AC A0A0G1JK17.1
E7AAF6_HELFC/1-88                  .m-------------------------TEMKSDDNSHYLIYRVLGISDKEGNLIDLYQNKGRFLYKHAGAFLEEATFACFKHKYNEA..EKMKIANTLGARPKTFEIDCLVNKDA------------------------------------------------------------------------------q.....
A9WD03_CHLAA/3-191                 .s-VTDKIRSLASKYAQELQHKIETRTKELESDDQSHYLIYKVLGIQDDEGRLIDLYQNKGRFLYKYAGAFLEEATFLCFKERFPHA..RRAQVENTSGQRPRQFHIDCLIDREAIEIKWRDATTDGDHIIKEHARIKVIKDHGFYPVRVMFFYPNREQAKRIQETIKTVYQGIGGACYTGDDAWEYVRTRT-g.....
Q5WJU5_BACSK/3-193                 ..VVENAIRTLAETYADRLNKQVLKRVKDMESDDKSHYLIYQVLKVTLEEGEMIDVYQNKGRFLYKYAGSFLEEAAILCFEYKFGEDalKKVKIANTLGQRPKTFEIDCLVGDNAYEIKWRDATTDGDHITKEHTRMQVIKDAGYMPNRIMFYYPNRTQAIRIQQTLETLYHGAGGHYYYGDSAWKHIYKVTD......
A1ZH81_9BACT/56-244                .l-LTQKIELLAEEYAANLQLKVLKRKQEMINDDNSHYLIYRVLGIARQEGRLIDEYQNTGRFLYKYAGSFLEEATSICLKFNNLKG..GKRFVKNTQGKKPKNFEIDFVDGQYAFEIKWRDATTDGDHITKEHTRIQVVKSQGYIPVRIMFYYPQRLQAQKIQATLKTLYTGVGGTYYVGDDAWSFVREHT-g.....
E7AAF7_HELFC/2-58                  pn-------------------------------------------------------------------------------------..---------------------------EIKWRDATTDGDHITKEHTRVKAIKQKGYKPIRVMFYYPQRAQAKKSKKRLK-------------------------lyi...
A0A0A1H260_9LACO/1-165             .m---KKIRTLASKYSEQLSKKVDIRIKEMQIDSKYHYLVYKVLGVTTKEGDLVDLYQNKGRFLYKYAGSFLEDAARLCFIEKYGEDnaVKIRIPNTLGDSPKTFEIDCLVNNDAREIKWRDATTDGDHVTKEHTRLQVVSEAGYKPIRVMFFYPNRKQGERK------------------------------itcvna
Q8DP93_STRR6/1-60                  ..---------------------------MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLEEAAVLSFNEKFGTE..--------------------------------------------------------------------------------------------------------nt....
K9Z263_CYAAP/1-43                  ..-------------------------------------------------------------------------------------..-------------------------------------------------------------MFYYPQREQAIKIQQTLETVYKGVNGEYYAGEQAWNFIRNRT-g.....
A7C3X0_9GAMM/2-70                  .k-IESEIRKLATKYSLQLQQKIDERVSEMKKDDLSHFLIYQVLGITNNEGHLIDVYQNKGRFLYKYAGSF----------------..--------------------------------------------------------------------------------------------------------......
G2Z232_FLABF/4-191                 li---QNIKNLAEVYSANLKGKIEARTEEMKADDNSHYLIYRVLGINLQEEQLIDQYQNTGRFLYKYAGSFLEEAASLCLNYKYPVG..IKTKVENTIGHRPKTFEIDFLNANDAIEVKWRVATTDGDHITKEHTRVQVIRNHGYKPIRVMFYYPQREQAIRIQQTLKTLYAGVEGAYYGGDEAWEYSKA---ysg...
Q8DP92_STRR6/1-43                  ..-------------------------------------------------------------------------------------..-------------------------------------------------------------MFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVT-g.....
A7C3W9_9GAMM/1-87                  ..---------------------------------------------------------------------------MCFLHAFPDS..GSVRIENTLGVKPKTFEIDCLTEQDAIEIKWRDATTDGDHITKEHTRIKVIANAGYKPIRIMFYYPNRQQAIRIQ-----------------------------ld....
Q6MU56_MYCMS/9-197                 ..VIYKKIKEIANKYSNKLNSQFQKRIVEMQNDDKSHHLIYRVLGIPYDVGNEIDYYQNQVRFLYKYAGSFLEEVTILCFKEKYPFA..SKTKVKNTISNIPKTFEIDCLVNNNAYEIKWRDATTDNDHIIKEHTRVKTLISYNYKPIRIMFYYPNRVNSMRIQETLETLYKGVGGEYYYGDSAWSYVKKLQE......
D1AUX4_STRM9/6-194                 .k-IYNKIKELADKYSNNLKFKVDERIEEMKNDDKSHYLIYRVLGVSTKEGELIDIYKNKGRFLYKYAGSFLEEATILCFKHKYTNS..KKIKVNNTLSNRPKTFEIDCLVDNKAYEIKWKDATTDGDHITKEHTRVKVIQSHGYTPIRIMFYYPNRSQAIKIQETLKTLYIGVNGQYYYSDLAWDYIKKETD......
A0A073CCY2_PLAAG/8-119             .s-LEEKIHNLAQKSSEALLKQINFRLEEMKVDDTSHFLIYRVLGITEQEGRLIDIYQNKGRFLYKYAGSFLEKATQLSFLEKYPDS..KSVKITNTLGSRPKTFEIDCLEGN--------------------------------------------------------------------------------rdt...
A0A0G1JK17_9BACT/7-196             .n-ISERINALASKYSQTLKTVMDARITEMEADDNSHYLLYRVLGVTDAEGKLIDEYQNKGRFLYKYAGSFLEEAAILCFEEKFPKA.kRKLRIPNKLGIRPTTFEIDCLVGKEAFEIKWRDATTDGDHITKEHTRIKNIKNASYKPIRIMFYYPNRKQAIRIQETLKTIYAGVGGEYYFGKGAWEIIKRKT-g.....
#=GC seq_cons                      .....ptIp.lAp..u.tL...h..RhpEMpsDDpSHaLIY+VLGls.pEGphID.YQNpGRFLYKYAGSFLE-As.lCF..+asps...phpl.NT.u.pP+sFEIDhL.sp.A.ElKW+sATTDsDHlhKEHsRhpsl.p.sahP.RlMFYYPpRtQAh+IQtplc.........................h.....
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