GenomeNet

Database: Pfam
Entry: RE_ApaLI
LinkDB: RE_ApaLI
Original site: RE_ApaLI 
#=GF ID   RE_ApaLI
#=GF AC   PF09499.9
#=GF DE   ApaLI-like restriction endonuclease
#=GF AU   Bateman A
#=GF SE   Bateman A
#=GF GA   25.00 25.00;
#=GF TC   34.70 34.60;
#=GF NC   23.00 22.70;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch -Z 26740544 -E 1000 --cpu 4 HMM pfamseq
#=GF TP   Family
#=GF RN   [1]
#=GF RM   9862476
#=GF RT   Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI,
#=GF RT   and SapI restriction-modification systems in Escherichia coli. 
#=GF RA   Xu SY, Xiao JP, Ettwiller L, Holden M, Aliotta J, Poh CL, Dalton
#=GF RA   M, Robinson DP, Petronzio TR, Moran L, Ganatra M, Ware J, Slatko
#=GF RA   B, Benner J; 
#=GF RL   Mol Gen Genet. 1998;260:226-231.
#=GF RN   [2]
#=GF RM   22638584
#=GF RT   Sequence, structure and functional diversity of PD-(D/E)XK
#=GF RT   phosphodiesterase superfamily.
#=GF RA   Steczkiewicz K, Muszewska A, Knizewski L, Rychlewski L, Ginalski
#=GF RA   K;
#=GF RL   Nucleic Acids Res. 2012;40:7016-7045.
#=GF DR   INTERPRO; IPR019036;
#=GF CC   This family includes R.ApaLI and R.XbaI restriction
#=GF CC   endonucleases. ApaLI recognises and cleaves the sequence GTGCAC.
#=GF SQ   28
#=GS E7AAF6_HELFC/1-88       AC E7AAF6.1
#=GS A0A0D6AEX0_9CHRO/1-43   AC A0A0D6AEX0.1
#=GS A0A151F381_9EURY/1-162  AC A0A151F381.1
#=GS A0A0C2Z7W7_9PROT/1-43   AC A0A0C2Z7W7.1
#=GS Q5WJU5_BACSK/3-193      AC Q5WJU5.1
#=GS A9WD03_CHLAA/3-191      AC A9WD03.1
#=GS A1ZH81_9BACT/56-244     AC A1ZH81.1
#=GS A0A176U5X9_9FIRM/3-49   AC A0A176U5X9.1
#=GS R9MCL9_9FIRM/5-192      AC R9MCL9.1
#=GS E7AAF7_HELFC/2-58       AC E7AAF7.1
#=GS A0A0A1H260_9LACO/1-165  AC A0A0A1H260.1
#=GS A0A0C2Z8I6_9PROT/1-43   AC A0A0C2Z8I6.1
#=GS K9Z263_CYAAP/1-43       AC K9Z263.1
#=GS Q8DP93_STRR6/1-60       AC Q8DP93.1
#=GS A7C3X0_9GAMM/2-70       AC A7C3X0.1
#=GS G6LTR6_STREE/1-60       AC G6LTR6.1
#=GS G2Z232_FLABF/4-191      AC G2Z232.1
#=GS G6LTR7_STREE/1-42       AC G6LTR7.1
#=GS A0A0D6AE65_9CHRO/1-110  AC A0A0D6AE65.1
#=GS Q8DP92_STRR6/1-43       AC Q8DP92.1
#=GS A7C3W9_9GAMM/1-87       AC A7C3W9.1
#=GS A0A0A6PBL5_9GAMM/1-43   AC A0A0A6PBL5.1
#=GS Q6MU56_MYCMS/9-197      AC Q6MU56.1
#=GS J1DGC0_STREE/3-89       AC J1DGC0.1
#=GS D1AUX4_STRM9/6-194      AC D1AUX4.1
#=GS A0A0G1JK17_9BACT/7-196  AC A0A0G1JK17.1
#=GS A0A073CCY2_PLAAG/8-119  AC A0A073CCY2.1
#=GS S9S0X7_9RHOB/1-154      AC S9S0X7.1
E7AAF6_HELFC/1-88                  ....m-------------------------TEMKSDDNSHYLIYRVLGISDKEGNLIDLYQNKGRFLYKHAGAFLEEATFACFKHKYNEA..EKMKIANTLGARPKTFEIDCL..VNK.DA------------------------------------------------------------------------------q..........
A0A0D6AEX0_9CHRO/1-43              .....-------------------------------------------------------------------------------------..---------------------..---.-------------------------------------MFYYLQREQAINIQQTLETVYKGVNGEYYAGEEAWNFIKTRT-g..........
A0A151F381_9EURY/1-162             .....---------------------------MDKNDLEHLSLYSILGFSEEEGLKIDLYQNIGRFLYKYAGALLEEATVVVLQNT-KEG..KSIRIPNTISQNPKKFVIDFFavSDN.KAHEIKWRDASTDGDHVRKEHNKIQCIVKAGMIPVRVMYYMPNRKQAIRIQKRIIAIYKEYG-EAYTDKGAWNYLLD---ytg........
A0A0C2Z7W7_9PROT/1-43              .....-------------------------------------------------------------------------------------..---------------------..---.-------------------------------------MFYYPNREQAMKIQSTLETLYKGIGGQYYYGNSAWYYVKDRT-g..........
Q5WJU5_BACSK/3-193                 .....VVENAIRTLAETYADRLNKQVLKRVKDMESDDKSHYLIYQVLKVTLEEGEMIDVYQNKGRFLYKYAGSFLEEAAILCFEYKFGEDalKKVKIANTLGQRPKTFEIDCL..VGD.NAYEIKWRDATTDGDHITKEHTRMQVIKDAGYMPNRIMFYYPNRTQAIRIQQTLETLYHGAGGHYYYGDSAWKHIYKVTD...........
A9WD03_CHLAA/3-191                 ....s-VTDKIRSLASKYAQELQHKIETRTKELESDDQSHYLIYKVLGIQDDEGRLIDLYQNKGRFLYKYAGAFLEEATFLCFKERFPHA..RRAQVENTSGQRPRQFHIDCL..IDR.EAIEIKWRDATTDGDHIIKEHARIKVIKDHGFYPVRVMFFYPNREQAKRIQETIKTVYQGIGGACYTGDDAWEYVRTRT-g..........
A1ZH81_9BACT/56-244                ....l-LTQKIELLAEEYAANLQLKVLKRKQEMINDDNSHYLIYRVLGIARQEGRLIDEYQNTGRFLYKYAGSFLEEATSICLKFNNLKG..GKRFVKNTQGKKPKNFEIDFV..DGQ.YAFEIKWRDATTDGDHITKEHTRIQVVKSQGYIPVRIMFYYPQRLQAQKIQATLKTLYTGVGGTYYVGDDAWSFVREHT-g..........
A0A176U5X9_9FIRM/3-49              ...sn---EKLIKIIKKYRDAFQKKIEERKLEMECDNNEHYVIYNVLGITD---------------------------------------..---------------------..---.--------------------------------------------------------------------------------tf.........
R9MCL9_9FIRM/5-192                 derlv-------KVILRYREELQKKIRERKIEMESDNNDHYILYSALGFTNEEGYQIDFQQNVGRFLYKYAGSLVEELAIKCFKLVHPDA.qEKVKLPNTIDQSPKTVEIDCL..IGK.RAIELKWKDATTDGDHIKKEHKRVRIIQKAGYTPIRIMFFEPNRDQAIRIQAKLKELYSDLGGEYYSGEKAWEYLKNDT-g..........
E7AAF7_HELFC/2-58                  ...pn-------------------------------------------------------------------------------------..---------------------..---.---EIKWRDATTDGDHITKEHTRVKAIKQKGYKPIRVMFYYPQRAQAKKSKKRLK-------------------------lyi........
A0A0A1H260_9LACO/1-165             ....m---KKIRTLASKYSEQLSKKVDIRIKEMQIDSKYHYLVYKVLGVTTKEGDLVDLYQNKGRFLYKYAGSFLEDAARLCFIEKYGEDnaVKIRIPNTLGDSPKTFEIDCL..VNN.DAREIKWRDATTDGDHVTKEHTRLQVVSEAGYKPIRVMFFYPNRKQGERK------------------------------itcvna.....
A0A0C2Z8I6_9PROT/1-43              .....-------------------------------------------------------------------------------------..---------------------..---.-------------------------------------MFYYPNREQAMKIQSTLETLYKGIGGQYYYGDSAWEYVKERT-g..........
K9Z263_CYAAP/1-43                  .....-------------------------------------------------------------------------------------..---------------------..---.-------------------------------------MFYYPQREQAIKIQQTLETVYKGVNGEYYAGEQAWNFIRNRT-g..........
Q8DP93_STRR6/1-60                  .....---------------------------MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLEEAAVLSFNEKFGTE..---------------------..---.--------------------------------------------------------------------------------nt.........
A7C3X0_9GAMM/2-70                  ....k-IESEIRKLATKYSLQLQQKIDERVSEMKKDDLSHFLIYQVLGITNNEGHLIDVYQNKGRFLYKYAGSF----------------..---------------------..---.--------------------------------------------------------------------------------...........
G6LTR6_STREE/1-60                  .....---------------------------MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLEEAAVLSFNEKFGTE..---------------------..---.--------------------------------------------------------------------------------nt.........
G2Z232_FLABF/4-191                 ...li---QNIKNLAEVYSANLKGKIEARTEEMKADDNSHYLIYRVLGINLQEEQLIDQYQNTGRFLYKYAGSFLEEAASLCLNYKYPVG..IKTKVENTIGHRPKTFEIDFL..NAN.DAIEVKWRVATTDGDHITKEHTRVQVIRNHGYKPIRVMFYYPQREQAIRIQQTLKTLYAGVEGAYYGGDEAWEYSKA---ysg........
G6LTR7_STREE/1-42                  .....-------------------------------------------------------------------------------------..---------------------..---.-------------------------------------MFYYPNRTQAIKIQQTLETLYNGIGGKY-YGDSAWEHLRAVT-g..........
A0A0D6AE65_9CHRO/1-110             .....---------------------------MKNDDTSHYLIYRVLGITSQEGQKIDEYQNIGRFLYNYAGSFLKEIASLCLLFNNPQG..KKTTVKNSFGTKPKTFEIDFL..DGN.DAIEIKWRDATTDRDHN---------------------------------------------------------------herayscksyt
Q8DP92_STRR6/1-43                  .....-------------------------------------------------------------------------------------..---------------------..---.-------------------------------------MFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVT-g..........
A7C3W9_9GAMM/1-87                  .....---------------------------------------------------------------------------MCFLHAFPDS..GSVRIENTLGVKPKTFEIDCL..TEQ.DAIEIKWRDATTDGDHITKEHTRIKVIANAGYKPIRIMFYYPNRQQAIRIQ-----------------------------ld.........
A0A0A6PBL5_9GAMM/1-43              .....-------------------------------------------------------------------------------------..---------------------..---.-------------------------------------MFYYPQREQAIKIQEILKTIYSGVDGEYYAGDEAWNYLTKI--sg.........
Q6MU56_MYCMS/9-197                 .....VIYKKIKEIANKYSNKLNSQFQKRIVEMQNDDKSHHLIYRVLGIPYDVGNEIDYYQNQVRFLYKYAGSFLEEVTILCFKEKYPFA..SKTKVKNTISNIPKTFEIDCL..VNN.NAYEIKWRDATTDNDHIIKEHTRVKTLISYNYKPIRIMFYYPNRVNSMRIQETLETLYKGVGGEYYYGDSAWSYVKKLQE...........
J1DGC0_STREE/3-89                  ...el--ERKIEKLAKKYSDNLNIKVQERVREMANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLEEAAVLCFNEKFGTE..---------------------..---.--------------------------------------------------------------------------------nt.........
D1AUX4_STRM9/6-194                 ....k-IYNKIKELADKYSNNLKFKVDERIEEMKNDDKSHYLIYRVLGVSTKEGELIDIYKNKGRFLYKYAGSFLEEATILCFKHKYTNS..KKIKVNNTLSNRPKTFEIDCL..VDN.KAYEIKWKDATTDGDHITKEHTRVKVIQSHGYTPIRIMFYYPNRSQAIKIQETLKTLYIGVNGQYYYSDLAWDYIKKETD...........
A0A0G1JK17_9BACT/7-196             ....n-ISERINALASKYSQTLKTVMDARITEMEADDNSHYLLYRVLGVTDAEGKLIDEYQNKGRFLYKYAGSFLEEAAILCFEEKFPKA.kRKLRIPNKLGIRPTTFEIDCL..VGK.EAFEIKWRDATTDGDHITKEHTRIKNIKNASYKPIRIMFYYPNRKQAIRIQETLKTIYAGVGGEYYFGKGAWEIIKRKT-g..........
A0A073CCY2_PLAAG/8-119             ....s-LEEKIHNLAQKSSEALLKQINFRLEEMKVDDTSHFLIYRVLGITEQEGRLIDIYQNKGRFLYKYAGSFLEKATQLSFLEKYPDS..KSVKITNTLGSRPKTFEIDCL..EGN.--------------------------------------------------------------------------------rdt........
S9S0X7_9RHOB/1-154                 ...ma--------------------------------------FAALGVSAEDATNIDLHQSISRLVYRHTGTMMEEVARHCLQRAFPGA..GRALIPNTVGDSPKKFEIDCL..VEDtDAIEIKWRDATTDGDHVRKEAQRVEATRDAGYTPVRLMFFEPKRPSAIRIQKNLAAHYRGVGGSYHKGDDAFRFIREQT-g..........
#=GC seq_cons                      ................................Mt.Ds.pHallYplLGls.p-u..ID.aQNhuRFLY+aAGuhhcchs.hsh..t...t..................................................................MFaYPpRtQAh+IQtpltthY.tht.th..sp.Aa.h.ht...t..........
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