GenomeNet

Database: Pfam
Entry: Rep_4
LinkDB: Rep_4
Original site: Rep_4 
#=GF ID   Rep_4
#=GF AC   PF05797.7
#=GF DE   Yeast trans-acting factor (REP1/REP2)
#=GF PI   Yeast_TAF; 
#=GF AU   Moxon SJ
#=GF SE   Pfam-B_7680 (release 8.0)
#=GF GA   25.00 25.00;
#=GF TC   30.10 29.00;
#=GF NC   19.80 19.60;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch -Z 80369284 -E 1000 --cpu 4 HMM pfamseq
#=GF TP   Family
#=GF RN   [1]
#=GF RM   12177044
#=GF RT   The 2 micron plasmid purloins the yeast cohesin complex: a
#=GF RT   mechanism for coupling plasmid partitioning and chromosome
#=GF RT   segregation? 
#=GF RA   Mehta S, Yang XM, Chan CS, Dobson MJ, Jayaram M, Velmurugan S; 
#=GF RL   J Cell Biol 2002;158:625-637.
#=GF DR   INTERPRO; IPR008897;
#=GF CC   This family consists of the yeast trans-acting factor B and C
#=GF CC   (REP1 and 2) proteins. The yeast plasmid stability system
#=GF CC   consists of two plasmid-coded proteins, Rep1 and Rep2, and a
#=GF CC   cis-acting locus, STB. The Rep proteins show both self- and
#=GF CC   cross-interactions in vivo and in vitro, and bind to the STB DNA
#=GF CC   with assistance from host factor(s). Within the yeast nucleus,
#=GF CC   the Rep1 and Rep2 proteins tightly associate with STB-containing
#=GF CC   plasmids into well organised plasmid foci that form a cohesive
#=GF CC   unit in partitioning. It is generally accepted that the
#=GF CC   protein-protein and DNA-protein interactions engendered by the
#=GF CC   Rep-STB system are central to plasmid partitioning. Point
#=GF CC   mutations in Rep1 that knock out interaction with Rep2 or with
#=GF CC   STB simultaneously block the ability of these Rep1 variants to
#=GF CC   support plasmid stability [1].
#=GF SQ   22
#=GS E7KWB6_YEASL/2-336      AC E7KWB6.1
#=GS U4C371_PEPDI/13-345     AC U4C371.1
#=GS W0W913_ZYGBA/1-90       AC W0W913.1
#=GS O43094_TORDE/35-257     AC O43094.1
#=GS A0A023Z886_YEASX/2-336  AC A0A023Z886.1
#=GS REP1_ZYGRO/11-354       AC P13778.1
#=GS REP1_ZYGBA/11-356       AC P13776.1
#=GS C5DT21_ZYGRC/11-354     AC C5DT21.1
#=GS REP1_ZYGBI/154-281      AC P13777.1
#=GS REP2_LACFM/1-154        AC P13742.1
#=GS U3XZL2_PEPDI/13-345     AC U3XZL2.1
#=GS H0H002_SACCK/13-314     AC H0H002.1
#=GS A5LQV4_STREE/1-30       AC A5LQV4.1
#=GS U4AI84_PEPDI/1-197      AC U4AI84.1
#=GS REP1_KLULC/32-243       AC P13775.1
#=GS O43094_TORDE/246-344    AC O43094.1
#=GS U4BF57_PEPDI/13-345     AC U4BF57.1
#=GS E7KJU5_YEASA/1-199      AC E7KJU5.1
#=GS Q01090_KLUWA/13-251     AC Q01090.1
#=GS REP1_YEAST/2-336        AC P03871.1
#=GS H1PIJ1_SACCK/2-336      AC H1PIJ1.1
#=GS U5TY33_9SACH/2-336      AC U5TY33.1
E7KWB6_YEASL/2-336                 ......................................................................................NGERLLACIKQCIMQHFQPMVYDESRCVIETTRGTFPVPDNYKKYKTLAFAFVGHVLNTDDTPVIEKEL..DWPDPALVYNTIVDRIINHpelsQFisvaFISQLKATIGEGL------DINVKGTLN.RRG....K.......GIRRPKGVFFRYMESPFVN-----TKVTAFFSYLrdynkiaseyHNNT----------KFILTFSCQ.AYWA.SGPNFSALKNVIRCSIIHEYISKFVEREQ...DKG---..H..IGDQelppEEDPSRELNNVQhEVNSLTE.QDA-.---EADEGLW.GEIDSLCEKWQSEAeDQTEAEI-----IADRIIGNSQRMANLKIRRTKFKSVLYHILKELIQSQGTVKVYRGSSFSHD-SIKISLHyEE-q..............................................................
U4C371_PEPDI/13-345                ......................................................................................NGERLLVCIKQCIMQHFQPMIYDESRCVIETTRGTFSVPDNYKKYKTLAFAFVGHVLNTDDTPVIEKEL..DWPDPALVYNTIVDRIRNHpelsQFisvaFISQLKAAIGEGL------DINVRGTLN.RRG....K.......GIRRPKGVFFRYMESPFVN-----TKVTAFFSYLrdynkiaseyHNNT----------KFILTFSCK.AYWA.SGPNFSALKNVIRCSIVHEYISRFVEREQ...--G---..L..IGDQeqprEENSSPELSNVQhEVNNLIE.QDA-.---AADEGLW.SEIDSLCEKWQSEAeDQAEAEI-----IADRIIGNSQRMANLKIRRTKFKSVLYHILKELIQSQGTVKVYRGSSFSHD-SIKISLHyEE-q..............................................................
W0W913_ZYGBA/1-90                  .....................................................................................m---------------------------------------------------------------------..-------------------....--....----------------------------.---....-.......------------------NSTNLRRELDEYYRKL..........QDASLTAESEGKLCKVKTLISCK.ALFGvKTLEPVVMAANCRIVWVGEAL--------...------..-..----....------------.-------.----.----------.--------------.-----------------------------------------------------------------------.---rgiavfdinpclqiggisdsekdk.......................................
O43094_TORDE/35-257                .................................................................................nkevr------AYLSECFFLLPHRVIYCPEHQCVVGMRGYMNVPDPYAEYPMLAIYFAKYRLRKYPFDILRNDI..TWPEPYVVLNTIMRRLRTH....RF...lKGNADAASIPDDIRKVIAPNLDIPSGME.GEL....L.......EVPYSERKLGANRVMKVFEGQDARLEMERFFDEL..........LGSS-------------------.----.-----------------------------...------..-..----....------------.-------.----.----------.--------------.-----------------------------------------------------------------------.---hselypdssvirvvsdyplrsqadvivfseylrklwyiekilvlmnmvpdsvrdrpdledpsi
A0A023Z886_YEASX/2-336             ......................................................................................NGERLLACIKQCIMQHFQPMVYDESRCVIETTRGTFPVPDNYKKYKTLAFAFVGHVLNTDDTPVIEKEL..DWPDPALVYNTIVDRIINHpelsQFisvaFISQLKATIGEGL------DINVKGTLN.RRG....K.......GIRRPKGVFFRYMESPFVN-----TKVTAFFSYLrdynkiaseyHNNT----------KFILTFSCQ.AYWA.SGPNFSALKNVIRCSIIHEYISKFVEREQ...DKG---..H..IGDQelppEEDPSRELNNVQhEVNSLTE.QDA-.---EADEGLW.GEIDSLCEKWQSEAeDQTEAEI-----IADRIIGNSQRMANLKIRRTKFKSVLYHILKELIQSQGTVKVYRGSSFSHD-SIKISLHyEE-q..............................................................
REP1_ZYGRO/11-354                  ......................................................................................RPDRELRMMNDVLMTYPYTVIHLPAQNMLSTAKGMVNIAENYRDYPILAIFYVKYLMKKLPYGVIPVNL..EWPEPYVVLNTILKRLKEH....KF....FANKDKEDFAERLHKLIAPDVSIPESRK.DEI....L.......GQQKKERVVTKTINENFLDPVNARPRLQRFFEKL..........HNGTLVENLEVGLCKVEILVSSK.AMLG.QSFKLQIMAANVRELWVGEMVCNMITNET...DYGFDE..G..GGDD....DEGSSVEVQNSQ.SASPGQD.QEAQ.RAPEAPETSS.QLFDKIVSALQDDP.DSAKAQLGQCRKLASYLLSHKREQEDFFMQTKDTRARLYLDLKGCLGSSWKFSIYRGVRCKQNGQLKVSLK.PSN...............................................................
REP1_ZYGBA/11-356                  ......................................................................................RPTKEMKMIFDVLMTFPYFAVHVPSKNILITPKGTVEIPENYQNYPILAIFYVKYLMKKNPYDLLPSTV..NWPEPYVVVNTITKRFQDH....KL....FANKNADVYVERLQNAIASGIKIPESKK.NER....L.......GQPKKTKNVTKEIEETFIDATNARKELDEYFRKL..........QDGTLTGDLEGGLCKVKTLISCK.ALFGgHTQELQFMATNVRKVWIGEIVCGMVSNKNaidDNDLEEeeRnaSGEQ....TTTAREESEALD.TTSNGLDaLNTQiNAIETEESFW.EAIRALHNELRTSP.----TQLEECRKAAVFLLGHKKILQTFTKQKDTARALFYINLKECLGTSWNLEYT----------------.---easdarkmaikgelq................................................
C5DT21_ZYGRC/11-354                ......................................................................................RPDRELRMMNDVLMTYPYTVIHLPAQNMLSTPKGMVNIAENYRDYPILAIFYVKYLMKKSPYQVIPVNL..EWPEPYVVLNTILKRLKEH....KF....FANKDKEDFAERLHKLIAPDVSIPESRK.DEI....L.......GQQKKERVVTKTISENFLDPVNARPRLQRFFEKL..........HNGTLVENLEVGLCKVEILVSSK.AMLG.QPLKLQFMAANVRELWVGEMVCNMITNET...DYGFDE..R..GGDD....DEGSSVEVQNSQ.SASPGQD.QEAQ.RAPEAPETSS.QLFDKIVSALQDDP.DSAKAQLGQCRKLANYLLSHKREQEDFFMQTKDTRARLYLDLKGCLGSSWKFSIYRGVRCKQNGQLKVSLK.PSN...............................................................
REP1_ZYGBI/154-281                 .................................................................vervvssqttsraaetrersp---------------------------------------------------------------------..-------------------....--....----------------------------.---....-.......----------------------------------..........-----------------------.----.-----------------------------...------..-..----....------------.---EHTE.ASTQ.EAETAEEALNkEEFWNLYRSLESKS.YISRENVPDIRLLVMCLKANLEIQGEFDRRRVGLKNRLYIILKDLVEEPWELRAYRGCRSADGLTLSVSFK.Y--kn.............................................................
REP2_LACFM/1-154                   meanpgelerfgkairkiwlyetimlgaekiakgnlseeststseseipntssqqdvisssehvsdslkfwevlnflevswvphsg---------------------------------------------------------------------..-------------------....--....----------------------------.---....-.......----------------------------------..........-----------------------.----.-----------------------------...------..-..----....------------.-------.----.----------.--------------.---ED--GVQHKLSQYVEKDPSTFEELKKRRLAAKNDVYGYVRQIMYSKGQVRIYRGVRCKPNGVLKVSL-.---fyg............................................................
U3XZL2_PEPDI/13-345                ......................................................................................NGERLLVCIKQCIMQHFQPMIYDESRCVIETTRGTFSVPDNYKKYKTLAFAFVGHVLNTDDTPVIEKEL..DWPDPALVYNTIVDRIRNHpelsQFisvaFISQLKAAIGEGL------DINVRGTLN.RRG....K.......GIRRPKGVFFRYMESPFVN-----TKVTAFFSYLrdynkiaseyHNNT----------KFILTFSCK.AYWA.SGPNFSALKNVIRCSIVHEYISRFVEREQ...--G---..L..IGDQeqprEENSSPELSNVQhEVNNLIE.QDA-.---AADEGLW.SEIDSLCEKWQSEAeDQAEAEI-----IADRIIGNSQRMANLKIRRTKFKSVLYHILKELIQSQGTVKVYRGSSFSHD-SIKISLHyEE-q..............................................................
H0H002_SACCK/13-314                ......................................................................................NGEKLLICIKQCIMEHFQPMIYVENRNVIQTTRGTFSVPDNYRNHKFLAFTFIGHILYTEDTPVIEKEW..DWPDPALVFNTVVDRITNHpelaQFisvsFISTLKATIGQGL------DMNVKGTLN.HKE....K.......GIRRPKTVSFRYMESPLVN-----KKVTKFFSYL..........RNYNKIAPEYGNNTKFTLKFSCQ.AYWA.SGPNFAALKNVIRCFIVHEYISEYVERER...TKYHIK..K..QGSW...aEEDHPRDLSKAQyEGNGLTE.KS--.--PNTDEELG.TKIDSLCEQWQSEAeDQADAEI-----LAKKIVGNSQRMANLKNSSHKV-------------------------------------.---qkypisy........................................................
A5LQV4_STREE/1-30                  ......................................................................................---------------------------------------------------------------------..-------------------....--....----------------------------.---....-.......----------------------------------..........-----------------------.----.-----------------------------...------..-..----....------------.-------.----.----------.--------------.--------------------------------------------LIQSQGTVKVYRGSSFSHD-SIKISLHyEEQ...............................................................
U4AI84_PEPDI/1-197                 ......................................................................................---------------------------------------------------------------------..-------------------....--....----------------------------.---....-.......------------MESPFVN-----TKVTAFFSYLrdynkiaseyHNNT----------KFILTFSCK.AYWA.SGPNFSALKNVIRCSIVHEYISRFVEREQ...--G---..L..IGDQeqprEENSSPELSNVQhEVNNLIE.QDA-.---AADEGLW.SEIDSLCEKWQSEAeDQAEAEI-----IADRIIGNSQRMANLKIRRTKFKSVLYHILKELIQSQGTVKVYRGSSFSHD-SIKISLHyEE-q..............................................................
REP1_KLULC/32-243                  ......................................................................iyralldsfkfkneyn--------------------AYDPLNNTIITQHGSYEVPEAFAQCTYLAQIYVSYKINSLPYQTYIKDL..EWISPAEVYKLIMERLQKN....KY....FLRKQVQA-MEKIKVLLCPSPELLENNYvDDN....LkisshkvTQRHPEKVYDLMTYQ------EIADGIDDFFSLL..........EMPNLS-LAFVNKTSIKLNISCSgANNH.IGSFIGRTARTIRHYWIKNAILEF-----...------..-..----....------------.-------.----.----------.--------------.-----------------------------------------------------------------------.---adykkktv.......................................................
O43094_TORDE/246-344               ......................................................................vrdrpdledpsivwdl---------------------------------------------------------------------..-------------------....--....----------------------------.---....-.......----------------------------------..........-----------------------.----.-----------------------------...------..-..----....------------.-------.----.----------.------LEDLQRQP.VYEPSQNERMTQVAALLKLWPDALEEFQQQRKDCRTFIYSKIRAWLPKKNKVKIYRGTSVLEDGSVKVSLN.---ftey...........................................................
U4BF57_PEPDI/13-345                ......................................................................................NGERLLVCIKQCIMQHFQPMIYDESRCVIETTRGTFSVPDNYKKYKTLAFAFVGHVLNTDDTPVIEKEL..DWPDPALVYNTIVDRIRNHpelsQFisvaFISQLKAAIGEGL------DINVRGTLN.RRG....K.......GIRRPKGVFFRYMESPFVN-----TKVTAFFSYLrdynkiaseyHNNT----------KFILTFSCK.AYWA.SGPNFSALKNVIRCSIVHEYISRFVEREQ...--G---..L..IGDQeqprEENSSPELSNVQhEVNNLIE.QDA-.---AADEGLW.SEIDSLCEKWQSEAeDQAEAEI-----IADRIIGNSQRMANLKIRRTKFKSVLYHILKELIQSQGTVKVYRGSSFSHD-SIKISLHyEE-q..............................................................
E7KJU5_YEASA/1-199                 ......................................................................................---------------------------------------------------------------------..-------------------....--....----------------------------.---....-.......------------MESPFVN-----TKVTAFFSYLrdynkiaseyHNNT----------KFILTFSCQ.AYWA.SGPNFSALKNVIRCSIIHEYISKFVEREQ...DKG---..H..IGDQelppEEDPSRELNNVQhEVNSLTE.QDA-.---EADEGLW.GEIDSLCEKWQSEAeDQTEAEI-----IADRIIGNSQRMANLKIRRTKFKSVLYHILKELIQSQGTVKVYRGSSFSHD-SIKISLHyEE-q..............................................................
Q01090_KLUWA/13-251                .................................................................................hdgyh------NCLKECCLVGTVDVLYVPQLKELVSPYGSTKLPKEYSEHPQLALFYYSYMINKNTASAARSELtfEWPKPYVVLNTIVKRLESH....IF....VVRNKYTQLPSIVKKHIGEGIECPTNDS.IEEltpfL.......DPRKRHPIIIRL---------GPKREALDFFTEL..........TDLEAAQSKYENLSKLEVRISCR.QWFG.SSLDVERSARTIRRLWLLEMVMSRAESIQ...QAA---..-..----....------------.-------.----.----------.--------------.-----------------------------------------------------------------------.---aaedqeptvdnesqgessgsgppidhaa...................................
REP1_YEAST/2-336                   ......................................................................................NGERLLACIKQCIMQHFQPMVYDESRCVIETTRGTFPVPDNYKKYKTLAFAFVGHVLNTDDTPVIEKEL..DWPDPALVYNTIVDRIINHpelsQFisvaFISQLKATIGEGL------DINVKGTLN.RRG....K.......GIRRPKGVFFRYMESPFVN-----TKVTAFFSYLrdynkiaseyHNNT----------KFILTFSCQ.AYWA.SGPNFSALKNVIRCSIIHEYISKFVEREQ...DKG---..H..IGDQelppEEDPSRELNNVQhEVNSLTE.QDA-.---EADEGLW.GEIDSLCEKWQSEAeDQTEAEI-----IADRIIGNSQRMANLKIRRTKFKSVLYHILKELIQSQGTVKVYRGSSFSHD-SIKISLHyEE-q..............................................................
H1PIJ1_SACCK/2-336                 ......................................................................................NGERLLVCIKQCIMQHFQPMVYDESRCVIETTRGTFPVPDNYKKYKTLAFAFVGHVLNTDDTPVIEKEL..DWPDPALVYNTIVDRIINHpelsQFisvaFISQLKATIGEGL------DINVKGTLN.RRG....K.......GIRRPKGVFFRYMESPFVN-----TKVTAFFSYLrdynkiaseyHNNT----------KFILTFSCQ.AYWA.SGPNFSALKNVIRCSIIHEYISKFVEREQ...DKG---..H..IGDQelppEEDPSRELNNVQhEVNSLTE.QDA-.---EADEGLW.GEIDSLCEKWQSEAeDQTEAEI-----IADRIIGNSQRMANLKIRRTKFKSVLYHILKELIQSQGTVKVYRGSSFSHD-SIKISLHyEE-q..............................................................
U5TY33_9SACH/2-336                 ......................................................................................NGERLLACIKQCIMQHFQPMVYDESRCVIETTRGTFPVPDNYKKYKTLAFAFVGHVLNTDDTPVIEKEL..DWPDPALVYNTIVDRIINHpelsQFisvaFISQLKATIGEGL------DINVKGTLN.RRG....K.......GIRRPKGVFFRYMESPFVN-----TKVTAFFSYLrdynkiaseyHNNT----------KFILTFSCQ.AYWA.SGPNFSALKNVIRCSIIHEYISKFVEREQ...DKG---..H..IGDQelppEEDPSRELNNVQhEVNSLTE.QDA-.---EADEGLW.GEIDSLCEKWQSEAeDQTEAEI-----IADRIIGNSQRMANLKIRRTKFKSVLYHILKELIQSQGTVKVYRGSSFSHD-SIKISLHyEE-q..............................................................
#=GC seq_cons                      .............................................................................................hh.pshh.h...hla..tpphl.o.+G.h.ls-sYtpa.hLAhhahtahhpp.sh.hh..pl..pWPcPhlVhNTIhcRl.pH....pa....hhpp.htshsptl......shpl.ts.p.tc.....h.......s..+.cthhht.hppshls.....pclstFFshL..........csso..........KhhlhhSCp.Ahau.pu.phphhtsslRp.hltEhlsphlppcp.....s.....h...Gp.....pps...-.pt.p.psssh.-.tps.....ts.Es...t.hcplhpphQscs.s.scupl.....lAshllupppphtshphp+pch+shlYh.LKthltSphpl+lYRGsphpps.slKlSL+....p..............................................................
//
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