GenomeNet

Database: Pfam
Entry: hNIFK_binding
LinkDB: hNIFK_binding
Original site: hNIFK_binding 
#=GF ID   hNIFK_binding
#=GF AC   PF12196.6
#=GF DE   FHA Ki67 binding domain of hNIFK
#=GF AU   Mistry J, Gavin OL
#=GF SE   pdb_2aff
#=GF GA   25.00 25.00;
#=GF TC   31.90 30.60;
#=GF NC   23.60 18.40;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch -Z 17690987 -E 1000 --cpu 4 HMM pfamseq
#=GF TP   Domain
#=GF WK   MKI67IP
#=GF RN   [1]
#=GF RM   16244663
#=GF RT   Sequential phosphorylation and multisite interactions
#=GF RT   characterize specific target recognition by the FHA domain of
#=GF RT   Ki67.
#=GF RA   Byeon IJ, Li H, Song H, Gronenborn AM, Tsai MD;
#=GF RL   Nat Struct Mol Biol. 2005;12:987-993.
#=GF DR   INTERPRO; IPR021043;
#=GF CC   This domain family is found in eukaryotes, and is approximately
#=GF CC   40 amino acids in length. The family is found in association
#=GF CC   with Pfam:PF00076. There are two conserved sequence motifs:
#=GF CC   TPVCTP and LERRKS. This domain is found on the human nucleolar
#=GF CC   protein hNIFK. It binds to the fork-head-associated domain of
#=GF CC   human Ki67. High-affinity binding requires sequential
#=GF CC   phosphorylation by two kinases, CDK1 and GSK3, yielding pThr238,
#=GF CC   pThr234 and pSer230. This interaction is involved in cell cycle
#=GF CC   regulation.
#=GF SQ   87
#=GS G1KD10_ANOCA/215-250      AC G1KD10.2
#=GS F7GRP8_MACMU/224-262      AC F7GRP8.1
#=GS G3N9N6_GASAC/244-280      AC G3N9N6.1
#=GS L5LZT6_MYODS/209-247      AC L5LZT6.1
#=GS H7BZL0_HUMAN/36-75        AC H7BZL0.1
#=GS H2PXL1_PONAB/192-231      AC H2PXL1.1
#=GS A0A091E432_FUKDA/237-275  AC A0A091E432.1
#=GS I3LAS7_PIG/185-224        AC I3LAS7.1
#=GS MK67I_XENTR/200-240       AC Q6GL69.1
#=GS S9XL53_9CETA/102-140      AC S9XL53.1
#=GS F7CHW8_ORNAN/209-246      AC F7CHW8.1
#=GS F1NT45_CHICK/210-247      AC F1NT45.2
#=GS G3N9P4_GASAC/227-263      AC G3N9P4.1
#=GS M7AHE7_CHEMY/114-145      AC M7AHE7.1
#=GS G3RRD3_GORGO/205-222      AC G3RRD3.1
#=GS F7H4M9_MACMU/197-236      AC F7H4M9.1
#=GS A0A096NAZ1_PAPAN/107-146  AC A0A096NAZ1.1
#=GS C9J808_HUMAN/122-161      AC C9J808.7
#=GS G1ST34_RABIT/229-268      AC G1ST34.1
#=GS G3N9N9_GASAC/208-244      AC G3N9N9.1
#=GS G3N9P2_GASAC/100-136      AC G3N9P2.1
#=GS H2PQ78_PONAB/225-264      AC H2PQ78.1
#=GS I3JM67_ORENI/208-244      AC I3JM67.1
#=GS G1NH99_MELGA/230-267      AC G1NH99.2
#=GS H0XGI4_OTOGA/174-212      AC H0XGI4.1
#=GS M3X1K8_FELCA/224-257      AC M3X1K8.1
#=GS H0Z1Z6_TAEGU/143-180      AC H0Z1Z6.1
#=GS G5AS30_HETGA/163-201      AC G5AS30.1
#=GS U3KFL7_FICAL/208-245      AC U3KFL7.1
#=GS A0A096MQS7_PAPAN/184-223  AC A0A096MQS7.1
#=GS M3WDX8_FELCA/229-268      AC M3WDX8.1
#=GS G3H134_CRIGR/254-292      AC G3H134.1
#=GS L9JRU4_TUPCH/188-227      AC L9JRU4.1
#=GS G5APY2_HETGA/132-170      AC G5APY2.1
#=GS H2SJC8_TAKRU/227-263      AC H2SJC8.1
#=GS G3WED0_SARHA/245-284      AC G3WED0.1
#=GS MK67I_HUMAN/227-266       AC Q9BYG3.1
#=GS MK67I_HUMAN/227-266       DR PDB; 2AFF B; 228-266;
#=GS F7F9F4_CALJA/227-266      AC F7F9F4.1
#=GS I3LKX8_PIG/195-234        AC I3LKX8.1
#=GS L9L012_TUPCH/128-159      AC L9L012.1
#=GS F7H4N1_MACMU/227-266      AC F7H4N1.1
#=GS G3RQP7_GORGO/227-266      AC G3RQP7.1
#=GS H3DKE3_TETNG/208-241      AC H3DKE3.1
#=GS E2RHG8_CANLF/234-272      AC E2RHG8.2
#=GS H0VA71_CAVPO/232-270      AC H0VA71.1
#=GS MK67I_BOVIN/230-269       AC Q3SZM1.1
#=GS M3ZDQ1_XIPMA/206-243      AC M3ZDQ1.1
#=GS M3XPP4_MUSPF/234-273      AC M3XPP4.1
#=GS F6VF09_MACMU/171-206      AC F6VF09.1
#=GS F7GFY5_MACMU/205-243      AC F7GFY5.1
#=GS S9WBS6_9CETA/58-94        AC S9WBS6.1
#=GS G1P747_MYOLU/231-269      AC G1P747.1
#=GS I3MSC9_ICTTR/255-293      AC I3MSC9.1
#=GS F6XSZ6_MONDO/246-285      AC F6XSZ6.2
#=GS G1QKG8_NOMLE/227-266      AC G1QKG8.1
#=GS F7FU02_MACMU/176-215      AC F7FU02.1
#=GS A0A0D9RVW7_CHLSB/227-266  AC A0A0D9RVW7.1
#=GS H2SJC7_TAKRU/227-263      AC H2SJC7.1
#=GS F7GF12_MONDO/195-220      AC F7GF12.1
#=GS L5JYK5_PTEAL/232-271      AC L5JYK5.1
#=GS W5PV04_SHEEP/230-269      AC W5PV04.1
#=GS H2QIN2_PANTR/227-266      AC H2QIN2.1
#=GS G5AWW6_HETGA/162-200      AC G5AWW6.1
#=GS MK67I_DANRE/204-241       AC Q8JIY8.2
#=GS H2SJC6_TAKRU/208-244      AC H2SJC6.1
#=GS F7ASK0_HORSE/190-229      AC F7ASK0.1
#=GS G1LEM7_AILME/231-270      AC G1LEM7.1
#=GS G3RY58_GORGO/199-238      AC G3RY58.1
#=GS G5B456_HETGA/169-207      AC G5B456.1
#=GS G3UGN5_LOXAF/250-289      AC G3UGN5.1
#=GS A0A0D9RXC7_CHLSB/218-257  AC A0A0D9RXC7.1
#=GS MK67I_MOUSE/250-288       AC Q91VE6.1
#=GS F7FU06_MACMU/179-218      AC F7FU06.1
#=GS U3I4Q5_ANAPL/158-195      AC U3I4Q5.1
#=GS G5BKC7_HETGA/237-275      AC G5BKC7.1
#=GS I3LDD0_PIG/192-231        AC I3LDD0.1
#=GS J9NXM6_CANLF/231-270      AC J9NXM6.1
#=GS W5LGQ5_ASTMX/229-264      AC W5LGQ5.1
#=GS G3TVD0_LOXAF/205-244      AC G3TVD0.1
#=GS A0A096MEC0_POEFO/206-243  AC A0A096MEC0.1
#=GS G3WEC9_SARHA/256-295      AC G3WEC9.1
#=GS W5M2A4_LEPOC/207-244      AC W5M2A4.1
#=GS F1RXX7_PIG/229-268        AC F1RXX7.2
#=GS F7GH18_MACMU/217-256      AC F7GH18.1
#=GS MK67I_RAT/206-242         AC Q5RJM0.1
#=GS V8NE15_OPHHA/209-244      AC V8NE15.1
#=GS A0A087X3X3_POEFO/206-243  AC A0A087X3X3.1
G1KD10_ANOCA/215-250                 ....SVNSEDPTPVCTPTFFERRKS.----QPEDSEDTEIILKSP...
F7GRP8_MACMU/224-262                 ....TVDTQGPTPVCTLTFLER-KS.EVAEMNDDDENNEIVFKQP...
G3N9N6_GASAC/244-280                 ..nl--SSIDVTPICTPSVLERRKS.---TVVDDDGDDEIILRMP...
L5LZT6_MYODS/209-247                 ....TVDSQGPTPVCTPTFLDKRKS.EVAEMN-DDEDNEIVFKQP...
H7BZL0_HUMAN/36-75                   ....TVDSQGPTPVCTPTFLERRKS.QVAELNDDDKDDEIVFKQP...
H2PXL1_PONAB/192-231                 ....TVDSQGPTPVCTPTFLERRKS.EVAELNDDDKDDEIVFKQP...
A0A091E432_FUKDA/237-275             ...t-GDSQSTTPVCTPTFLEKRKS.EVAGMN-DDKDDEIVFKQP...
I3LAS7_PIG/185-224                   ....TVDSQGPTPVCTPTFLEKRKS.EVAKMNDDDKDHEIVFKQP...
MK67I_XENTR/200-240                  ....SVSSQDPTPVCTPTVLERRKSvRLEQNDEDDDDGEVVLKLP...
S9XL53_9CETA/102-140                 ..sr--KRQGPTPVCTPTFLEKRKS.EAAAMT-DDKDDEIVFKQP...
F7CHW8_ORNAN/209-246                 ....TVDSQAPTPVCTPTFLERRKS.VALGMS--EEDNEIILKLP...
F1NT45_CHICK/210-247                 ....SLTSQDPTPVCTPTVLERRKA.--QQADDDPDDNEIIIKLP...
G3N9P4_GASAC/227-263                 ....SMCSDDVTPICTPSVLERRKS.---TVVDDDGDDEIILRMP...
M7AHE7_CHEMY/114-145                 ...f-----DPTPVCTPTALERRKS.QPAEAD----EDEITIKLP...
G3RRD3_GORGO/205-222                 ....TVGSQGPTPVCTSTFLER---.-------------------...
F7H4M9_MACMU/197-236                 ....TADTQGLTPVCTSTFLERGKS.EVAVMNNDDEDNEIVFKKP...
A0A096NAZ1_PAPAN/107-146             ....TADSQGPTPVCTPTFLERRKS.EVAELNDDDKDNEIVFKQP...
C9J808_HUMAN/122-161                 ....TVDSQGPTPVCTPTFLERRKS.QVAELNDDDKDDEIVFKQP...
G1ST34_RABIT/229-268                 ....TVDSQGPTPVCTPSYLERRKS.EAAEMKDDEKDDEIVFRQP...
G3N9N9_GASAC/208-244                 ....SMCSDDVTPICTPSVLERRKS.---TVVDDDGDDEIILRMP...
G3N9P2_GASAC/100-136                 ....SMCSDDVTPICTPSVLERRKS.---TVVDDDGDDEIILRMP...
H2PQ78_PONAB/225-264                 ....TVDSQGPTPVCTPTFLERRKS.EVAELNDDDKDDEIVFKQP...
I3JM67_ORENI/208-244                 ....SSCTDDSTPVCTPSFLERRKS.---TAAENDADDEIIIKMP...
G1NH99_MELGA/230-267                 ..cl--NSQDPTPVCTPTVLERRKA.--QQADDDTDDNEIMIKLP...
H0XGI4_OTOGA/174-212                 ....TVDSQGPTPVCTPAFLEKRKS.EVAAMN-DDEDNEIVFKQP...
M3X1K8_FELCA/224-257                 ...l--ASQGPTPVCTPTVLEKRKS.EAAKMNVDDKEN-------pv.
H0Z1Z6_TAEGU/143-180                 ....SLSSQDPTPVCTPAVLERRKA.--AQMDGDTEDDEITLKLP...
G5AS30_HETGA/163-201                 ....TVDSQSATPACAPTLLEKRKS.EVAEMK-GDKDDEIVFKQP...
U3KFL7_FICAL/208-245                 ....SLSSQDPTPVCTPAVLERRKV.--ARMDDDTEDDEVILRLP...
A0A096MQS7_PAPAN/184-223             ....TVDIYGPTLVCTSTFLKKSKS.EVAEMNDDDEDNEIVFKQP...
M3WDX8_FELCA/229-268                 ....TVDSQGPTPVCTPTFLEKRKS.EVAKMNVDDKDNEIVFKQP...
G3H134_CRIGR/254-292                 ....SVDSQGPTPVCTPTFLERRKS.EVTEIN-DSKDDEVIFKQP...
L9JRU4_TUPCH/188-227                 ....TVDSQGPTPVCTPTVLEKRKS.EVTEMNDDDNDNEIVFKQP...
G5APY2_HETGA/132-170                 ....TVDSQSATPVYTPTFLEKRKS.EVAEMN-DDKDDEIVFKQP...
H2SJC8_TAKRU/227-263                 ttvp----TDATPVCTPSVLEKRKS.---MVVDDNADDEVVVKMP...
G3WED0_SARHA/245-284                 ....SMDSQGPTPICSPTFLERRKA.QAAEMNDDDEDNEIVLKLP...
MK67I_HUMAN/227-266                  ....TVDSQGPTPVCTPTFLERRKS.QVAELNDDDKDDEIVFKQP...
#=GR MK67I_HUMAN/227-266       SS    ....X--TTS------HHHHHHHHH.HHHH--GGGGS-SEEESS-...
F7F9F4_CALJA/227-266                 ....TVDSQGPTPVCTPAFLERRKS.EVTGMDEDDKDNEIVFKQP...
I3LKX8_PIG/195-234                   ....TVDSQGPTPVCTPTFLEKRKS.EVAKMNDDDKDHEIVFKQP...
L9L012_TUPCH/128-159                 ...r-LDSQGPTPVRTPTFLEEQKS.EVAEIKDDDKE--------...
F7H4N1_MACMU/227-266                 ....TVDSQGPTPVCTPTFLERRKS.EVAELNDDDKDNEIVFKEP...
G3RQP7_GORGO/227-266                 ....TVDSQGPTPVCTPTFLERRKS.QVAELNDDDKDDEIVFKQP...
H3DKE3_TETNG/208-241                 ....STCSEDATPVCTPSVLERRKS.MSEEPSEEEEEN-------k..
E2RHG8_CANLF/234-272                 .vfi----NGSTPVCTPAFLEKRKS.EAAKINVDDEDNEIVFKQP...
H0VA71_CAVPO/232-270                 ....TVDSQSATPVCTPTFLEKRKS.EVAGVN-DDKDDEIVFRQP...
MK67I_BOVIN/230-269                  ....AVDSQGPTPVCTPAFLEKRKS.EVAKMNDDDKDNEIVFKQP...
M3ZDQ1_XIPMA/206-243                 ....SSCSDQTTPVCTPSFLERRKS.--TVVLDDEEDSEIILKMP...
M3XPP4_MUSPF/234-273                 ....TVDSQGPTPVCTPTFLEKRKS.EVAKMNVDDKDNEIIFKQP...
F6VF09_MACMU/171-206                 ....TVDTQGPTPVCTSTFLGR---.-LAEMNDDNEENEILFKQP...
F7GFY5_MACMU/205-243                 ....TVDTQGPTPVCTLTFLER-KS.EVAEMNDDDENNEIVFKQP...
S9WBS6_9CETA/58-94                   ....AVDSQGPRPVCTPTFPEKQKS.GADAMT-EDKDEEI-----tss
G1P747_MYOLU/231-269                 ....TVDSQGPTPVCTPTFLDKRKS.EVAEMN-DDEDNEIVFKQP...
I3MSC9_ICTTR/255-293                 ....SIDSQGPTPVCTPTFLEKRKS.EVVGMD-DDEDDEIVFKLP...
F6XSZ6_MONDO/246-285                 ....TVDSQGATPICSPTFLERRKA.QAAEMNDDDDDNEIIFKLP...
G1QKG8_NOMLE/227-266                 ....TVDSQGPTPVCTPTFLERRKS.EVAELNDDDKDDEIIFKQP...
F7FU02_MACMU/176-215                 ....TADTQGPTPVCTSTFLERGKS.EASEMNGDDEDNEIVFKQ-s..
A0A0D9RVW7_CHLSB/227-266             ....TVDSQGPTPVCTPTFLERRKS.EVAELNDDDKDNEIVFKQP...
H2SJC7_TAKRU/227-263                 gqdw----KDATPVCTPSVLEKRKS.---MVVDDNADDEVVVKMP...
F7GF12_MONDO/195-220                 ....TVASQGATPICSPTFLERRKA.QAVEM--------------...
L5JYK5_PTEAL/232-271                 ....TVDSQGPTPVCTPTFLEKRKS.EAAEMNDDDKDNEIVFKQP...
W5PV04_SHEEP/230-269                 ....AVDSQGPTPVCTPTFLEKRKS.EVAKMNDDDKDNEIVFKHP...
H2QIN2_PANTR/227-266                 ....TVDSQGPTPVCTPTFLERRKS.QVAELNDDDKDDEIVFKQP...
G5AWW6_HETGA/162-200                 ....TVDSQSATPVCTPTFLEKRKS.EVAEMN-DDKDDEIVFKQP...
MK67I_DANRE/204-241                  ....SVCSEDVTPVCTPSLLERRKS.--LRVEDDDVDDEIVIKV-k..
H2SJC6_TAKRU/208-244                 ....SSCSEDATPVCTPSVLEKRKS.---MVVDDNADDEVVVKMP...
F7ASK0_HORSE/190-229                 ....TADSQGPTPVCTPTFLEKRKS.EVATMKDDDKDNEIVFKQP...
G1LEM7_AILME/231-270                 ....TVDSQGPTPVCTPTFLEKRKS.EVAKMNVDDKDNEIVFKQP...
G3RY58_GORGO/199-238                 ...s-TKGHGLTPVCTPTFLERRKS.EVAEMNDDDEANEIVFKQP...
G5B456_HETGA/169-207                 ....TVDSQSATPVCTPTLLETRKS.EVAEMN-DDKDDEIVFKQP...
G3UGN5_LOXAF/250-289                 ....STNSQGSTPVCTPTFLERRKC.EVAEMNDDEEDKEIIFKQP...
A0A0D9RXC7_CHLSB/218-257             ....TADTQGPTPVCTSMFLERGKS.EVAVMNNDDEDNERVFKKP...
MK67I_MOUSE/250-288                  ....SVDSQGPTPVCTPTFLERRKS.QVMEVG-GDKDDEIILKLP...
F7FU06_MACMU/179-218                 ....TADTQGPTPVCTSTFLERGKS.EASEMNGDDEDNEIVFKQ-s..
U3I4Q5_ANAPL/158-195                 ....SLSSQDPTPVCTPTVLERRQA.--HQADDDAEDKEITLKLP...
G5BKC7_HETGA/237-275                 ....TVDSQSATAVCTPVFLEKRKS.EVAEMN-DDKDDETAFKQP...
I3LDD0_PIG/192-231                   ....TVDSQGPTPVCTPTFLEKRKS.EVAKMNDDDKDHEIVFKQP...
J9NXM6_CANLF/231-270                 ....TVDSQGSTPVCTPAFLEKRKS.EAAKINVDDEDNEIVFKQP...
W5LGQ5_ASTMX/229-264                 .vpt-----DATPVCTPSILERRKS.--IRVEDDDVDDEIVLK--tq.
G3TVD0_LOXAF/205-244                 ....STNSQGSTPVCTPTFLERRKC.EVAEMNDDEEDKEIIFKQP...
A0A096MEC0_POEFO/206-243             ....SSCSDQTTPVCTPSFLERRKS.--TVVLDDEEDSEIILKMP...
G3WEC9_SARHA/256-295                 ....SMDSQGPTPICSPTFLERRKA.QAAEMNDDDEDNEIVLKLP...
W5M2A4_LEPOC/207-244                 ....TTDSLDVTPVCTPTVLEKRKS.--MKVEDSDGDDEIIIK--at.
F1RXX7_PIG/229-268                   ....TVDSQGPTPVCTPTFLEKRKS.EVAKMNDDDKDHEIVFKQP...
F7GH18_MACMU/217-256                 ....TADTQGLTPVCTSTFLERGKS.EVAVMNNDDEDNEIVFKKP...
MK67I_RAT/206-242                    ....SEANQDPTPVCTPTFLERRKS.QLMEIN---DDDEIILKLP...
V8NE15_OPHHA/209-244                 ....SVDDEDPTPVCTPTFFERRKS.----QPEDNEDDEIILKLP...
A0A087X3X3_POEFO/206-243             ....SSCSDQTTPVCTPSFLERRKS.--TVVLDDEEDSEIILKMP...
#=GC SS_cons                         ....X--TTS------HHHHHHHHH.HHHH--GGGGS-SEEESS-...
#=GC seq_cons                        ....olDSQGPTPVCTPTFLERRKS.psAchs-DDcDsEIVFK.P...
//
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