GenomeNet

Database: Pfam
Entry: hNIFK_binding
LinkDB: hNIFK_binding
Original site: hNIFK_binding 
#=GF ID   hNIFK_binding
#=GF AC   PF12196.3
#=GF DE   FHA Ki67 binding domain of hNIFK
#=GF AU   Mistry J, Gavin OL
#=GF SE   pdb_2aff
#=GF GA   25.00 25.00;
#=GF TC   29.00 27.90;
#=GF NC   20.40 19.60;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
#=GF TP   Domain
#=GF WK   MKI67IP
#=GF RN   [1]
#=GF RM   16244663
#=GF RT   Sequential phosphorylation and multisite interactions
#=GF RT   characterize specific target recognition by the FHA domain of
#=GF RT   Ki67.
#=GF RA   Byeon IJ, Li H, Song H, Gronenborn AM, Tsai MD;
#=GF RL   Nat Struct Mol Biol. 2005;12:987-993.
#=GF DR   INTERPRO; IPR021043;
#=GF CC   This domain family is found in eukaryotes, and is approximately
#=GF CC   40 amino acids in length. The family is found in association
#=GF CC   with Pfam:PF00076. There are two conserved sequence motifs:
#=GF CC   TPVCTP and LERRKS. This domain is found on the human nucleolar
#=GF CC   protein hNIFK. It binds to the fork-head-associated domain of
#=GF CC   human Ki67. High-affinity binding requires sequential
#=GF CC   phosphorylation by two kinases, CDK1 and GSK3, yielding pThr238,
#=GF CC   pThr234 and pSer230. This interaction is involved in cell cycle
#=GF CC   regulation.
#=GF SQ   73
#=GS F7F9F4_CALJA/227-267  AC F7F9F4.1
#=GS H2PQ78_PONAB/225-265  AC H2PQ78.1
#=GS MK67I_MOUSE/250-289   AC Q91VE6.1
#=GS H0XGI4_OTOGA/174-213  AC H0XGI4.1
#=GS F6XSY9_MONDO/219-259  AC F6XSY9.1
#=GS C1BG54_ONCMY/207-245  AC C1BG54.1
#=GS F6RAF3_MONDO/233-273  AC F6RAF3.1
#=GS F6RAG3_MONDO/236-276  AC F6RAG3.1
#=GS G3TVD0_LOXAF/205-245  AC G3TVD0.1
#=GS C1BK47_OSMMO/206-244  AC C1BK47.1
#=GS MK67I_XENTR/200-241   AC Q6GL69.1
#=GS F7CHW0_ORNAN/204-242  AC F7CHW0.1
#=GS G3N9P2_GASAC/100-137  AC G3N9P2.1
#=GS G3RRD3_GORGO/205-222  AC G3RRD3.1
#=GS G3H134_CRIGR/254-293  AC G3H134.1
#=GS G5B456_HETGA/169-208  AC G5B456.1
#=GS H2SJC8_TAKRU/228-264  AC H2SJC8.1
#=GS F7FU06_MACMU/179-219  AC F7FU06.1
#=GS G5AS30_HETGA/163-202  AC G5AS30.1
#=GS H2QIN2_PANTR/227-267  AC H2QIN2.1
#=GS H0VA71_CAVPO/232-271  AC H0VA71.1
#=GS F1NT45_CHICK/209-247  AC F1NT45.1
#=GS F7GFY5_MACMU/205-244  AC F7GFY5.1
#=GS G1QKG8_NOMLE/227-267  AC G1QKG8.1
#=GS F6VF09_MACMU/171-207  AC F6VF09.1
#=GS G5AWW6_HETGA/162-201  AC G5AWW6.1
#=GS F7FU02_MACMU/176-216  AC F7FU02.1
#=GS G5E1H6_9PIPI/156-180  AC G5E1H6.1
#=GS B5X6J4_SALSA/207-245  AC B5X6J4.1
#=GS A8WHZ8_DANRE/204-242  AC A8WHZ8.1
#=GS MK67I_DANRE/204-242   AC Q8JIY8.2
#=GS G3WED0_SARHA/245-285  AC G3WED0.1
#=GS H3DKE3_TETNG/208-241  AC H3DKE3.1
#=GS G5BKC7_HETGA/237-276  AC G5BKC7.1
#=GS F7H4M9_MACMU/197-237  AC F7H4M9.1
#=GS G1ST34_RABIT/229-269  AC G1ST34.1
#=GS H0Z1Z6_TAEGU/143-181  AC H0Z1Z6.1
#=GS H7BZL0_HUMAN/36-76    AC H7BZL0.1
#=GS A7E1T9_PIG/229-269    AC A7E1T9.1
#=GS G7NB31_MACMU/227-267  AC G7NB31.1
#=GS G3N9N6_GASAC/244-281  AC G3N9N6.1
#=GS G3RQP7_GORGO/227-267  AC G3RQP7.1
#=GS G1KD10_ANOCA/206-244  AC G1KD10.1
#=GS MK67I_XENLA/201-243   AC Q7SYS2.2
#=GS G9KAQ9_MUSPF/234-274  AC G9KAQ9.1
#=GS H2SJC7_TAKRU/228-264  AC H2SJC7.1
#=GS MK67I_BOVIN/230-270   AC Q3SZM1.1
#=GS H2SJC6_TAKRU/208-245  AC H2SJC6.1
#=GS F7GF12_MONDO/195-220  AC F7GF12.1
#=GS C9J808_HUMAN/122-162  AC C9J808.1
#=GS F7ASK0_HORSE/190-230  AC F7ASK0.1
#=GS E2RHG8_CANFA/256-295  AC E2RHG8.1
#=GS F7H4N1_MACMU/227-267  AC F7H4N1.1
#=GS F7CHW8_ORNAN/209-247  AC F7CHW8.1
#=GS F7GH18_MACMU/217-257  AC F7GH18.1
#=GS D2HHW5_AILME/175-215  AC D2HHW5.1
#=GS MK67I_HUMAN/227-267   AC Q9BYG3.1
#=GS MK67I_HUMAN/227-267   DR PDB; 2AFF B; 228-267;
#=GS G3RY58_GORGO/199-238  AC G3RY58.1
#=GS G5APY2_HETGA/132-171  AC G5APY2.1
#=GS G1LEM7_AILME/231-271  AC G1LEM7.1
#=GS B1MTB3_CALMO/226-266  AC B1MTB3.1
#=GS F1RXX7_PIG/149-189    AC F1RXX7.1
#=GS G3N9P4_GASAC/227-264  AC G3N9P4.1
#=GS F6XSZ6_MONDO/206-246  AC F6XSZ6.1
#=GS G1NH99_MELGA/230-268  AC G1NH99.2
#=GS F7GRP8_MACMU/224-263  AC F7GRP8.1
#=GS G1P747_MYOLU/231-270  AC G1P747.1
#=GS MK67I_RAT/206-243     AC Q5RJM0.1
#=GS G3UGN5_LOXAF/250-290  AC G3UGN5.1
#=GS H2PXL1_PONAB/192-232  AC H2PXL1.1
#=GS G3WEC9_SARHA/256-296  AC G3WEC9.1
#=GS H9G0R4_MACMU/227-267  AC H9G0R4.1
#=GS G3N9N9_GASAC/208-245  AC G3N9N9.1
F7F9F4_CALJA/227-267             ......TVDSQGPTPVCTPAFLERRKSEVTGM..DEDDKDN.EIVFKQPV...
H2PQ78_PONAB/225-265             ......TVDSQGPTPVCTPTFLERRKSEVAEL..NDDDKDD.EIVFKQPI...
MK67I_MOUSE/250-289              ......SVDSQGPTPVCTPTFLERRKSQVMEV..GGD-KDD.EIILKLPV...
H0XGI4_OTOGA/174-213             ......TVDSQGPTPVCTPAFLEKRKSEVAAM..NDD-EDN.EIVFKQP-k..
F6XSY9_MONDO/219-259             ......TVDSQGATPICSPTFLERRKAQAAEM..NDDDDDN.EIIFKLPP...
C1BG54_ONCMY/207-245             ......STCSEDVTPVCTPSVLERRRSMKTN-..-EDDSDG.EIIIKN--tp.
F6RAF3_MONDO/233-273             ......TVDNEGATPICSPTFLERRKAQAAEM..NDDDDDN.EIIFKLPP...
F6RAG3_MONDO/236-276             ......TVDNEGATPICSPTFLERRKAQAAEM..NDDDDDN.EIIFKLPP...
G3TVD0_LOXAF/205-245             ......STNSQGSTPVCTPTFLERRKCEVAEM..NDDEEDK.EIIFKQPV...
C1BK47_OSMMO/206-244             ......STCSEDTTPVCTPSFMERRKSARVT-..-DDDVDD.EIVIN---atp
MK67I_XENTR/200-241              ......SVSSQDPTPVCTPTVLERRKSVRLEQ..NDEDDDDgEVVLKLPQ...
F7CHW0_ORNAN/204-242             .....s-NFSQAPTPVCTPTFLERRKSVALGM..SEE--DN.EIILKLPP...
G3N9P2_GASAC/100-137             ......SMCSDDVTPICTPSVLERRKSTVVD-..--DDGDD.EIILRMP-s..
G3RRD3_GORGO/205-222             ......TVGSQGPTPVCTSTFLER--------..-------.--------...
G3H134_CRIGR/254-293             ......SVDSQGPTPVCTPTFLERRKSEVTEI..NDS-KDD.EVIFKQPV...
G5B456_HETGA/169-208             ......TVDSQSATPVCTPTLLETRKSEVAEM..NDD-KDD.EIVFKQP-a..
H2SJC8_TAKRU/228-264             ..tvpt-----DATPVCTPSVLEKRKSMVVD-..--DNADD.EVVVKMP-a..
F7FU06_MACMU/179-219             .....t-ADTQGPTPVCTSTFLERGKSEASEM..NGDDEDN.EIVFKQS-i..
G5AS30_HETGA/163-202             ......TVDSQSATPACAPTLLEKRKSEVAEM..KGD-KDD.EIVFKQPV...
H2QIN2_PANTR/227-267             ......TVDSQGPTPVCTPTFLERRKSQVAEL..NDDDKDD.EIVFKQPI...
H0VA71_CAVPO/232-271             ......TVDSQSATPVCTPTFLEKRKSEVAGV..NDD-KDD.EIVFRQPV...
F1NT45_CHICK/209-247             ......SLTSQDPTPVCTPTVLERRKAQQAD-..-DDPDDN.EIIIKLPP...
F7GFY5_MACMU/205-244             ......TVDTQGPTPVCTLTFLER-KSEVAEM..NDDDENN.EIVFKQPI...
G1QKG8_NOMLE/227-267             ......TVDSQGPTPVCTPTFLERRKSEVAEL..NDDDKDD.EIIFKQPI...
F6VF09_MACMU/171-207             ......TVDTQGPTPVCTSTFLGR----LAEM..NDDNEEN.EILFKQPI...
G5AWW6_HETGA/162-201             ......TVDSQSATPVCTPTFLEKRKSEVAEM..NDD-KDD.EIVFKQPV...
F7FU02_MACMU/176-216             .....t-ADTQGPTPVCTSTFLERGKSEASEM..NGDDEDN.EIVFKQS-i..
G5E1H6_9PIPI/156-180             ......SISSQDPTPVCTPTVLERRKSVRLE-..-------.--------...
B5X6J4_SALSA/207-245             ......STCSEDVTPVCTPSVLERRRSMKIN-..-EDDSDC.EIIIKN--tp.
A8WHZ8_DANRE/204-242             ......SVCSEDVTPVCTPSLLERRKSLRVE-..-DDDVDD.EIVIKVKP...
MK67I_DANRE/204-242              ......SVCSEDVTPVCTPSLLERRKSLRVE-..-DDDVDD.EIVIKVKP...
G3WED0_SARHA/245-285             ......SMDSQGPTPICSPTFLERRKAQAAEM..NDDDEDN.EIVLKLPP...
H3DKE3_TETNG/208-241             ......STCSEDATPVCTPSVLERRKSMSEEP..SEEEE--.--------enk
G5BKC7_HETGA/237-276             ......TVDSQSATAVCTPVFLEKRKSEVAEM..NDD-KDD.ETAFKQP-a..
F7H4M9_MACMU/197-237             .....t-ADTQGLTPVCTSTFLERGKSEVAVM..NNDDEDN.EIVFKKPI...
G1ST34_RABIT/229-269             ......TVDSQGPTPVCTPSYLERRKSEAAEM..KDDEKDD.EIVFRQPV...
H0Z1Z6_TAEGU/143-181             ......SLSSQDPTPVCTPAVLERRKAAQMD-..-GDTEDD.EITLKLP-s..
H7BZL0_HUMAN/36-76               ......TVDSQGPTPVCTPTFLERRKSQVAEL..NDDDKDD.EIVFKQPI...
A7E1T9_PIG/229-269               ......TVDSQGPTPVCTPTFLEKRKSEVAKM..NDDDKDH.EIVFKQPV...
G7NB31_MACMU/227-267             ......TVDSQGPTPVCTPTFLERRKSEVAEL..NDDDKDN.EIVFKQPI...
G3N9N6_GASAC/244-281             ....nl--SSIDVTPICTPSVLERRKSTVVD-..--DDGDD.EIILRMP-s..
G3RQP7_GORGO/227-267             ......TVDSQGPTPVCTPTFLERRKSQVAEL..NDDDKDD.EIVFKQPI...
G1KD10_ANOCA/206-244             sevspv----QDPTPVCTPTFFERRKSQP---..-EDSEDT.EIILKSP-s..
MK67I_XENLA/201-243              ......SVSSQDPTPVCTPTVLERRKSVRLEQieDEEEDDD.EVVLKLPQ...
G9KAQ9_MUSPF/234-274             ......TVDSQGPTPVCTPTFLEKRKSEVAKM..NVDDKDN.EIIFKQPI...
H2SJC7_TAKRU/228-264             ...qdw----KDATPVCTPSVLEKRKSMVVD-..--DNADD.EVVVKMP-a..
MK67I_BOVIN/230-270              .....a-VDSQGPTPVCTPAFLEKRKSEVAKM..NDDDKDN.EIVFKQPV...
H2SJC6_TAKRU/208-245             ......SSCSEDATPVCTPSVLEKRKSMVVD-..--DNADD.EVVVKMP-a..
F7GF12_MONDO/195-220             ......TVASQGATPICSPTFLERRKAQAVE-..-------.--------m..
C9J808_HUMAN/122-162             ......TVDSQGPTPVCTPTFLERRKSQVAEL..NDDDKDD.EIVFKQPI...
F7ASK0_HORSE/190-230             .....t-ADSQGPTPVCTPTFLEKRKSEVATM..KDDDKDN.EIVFKQPI...
E2RHG8_CANFA/256-295             ...lky----QGSTPVCTPAFLEKRKSEAAKI..NVDDEDN.EIVFKQPV...
F7H4N1_MACMU/227-267             ......TVDSQGPTPVCTPTFLERRKSEVAEL..NDDDKDN.EIVFKEPI...
F7CHW8_ORNAN/209-247             ......TVDSQAPTPVCTPTFLERRKSVALGM..SEE--DN.EIILKLPP...
F7GH18_MACMU/217-257             .....t-ADTQGLTPVCTSTFLERGKSEVAVM..NNDDEDN.EIVFKKPI...
D2HHW5_AILME/175-215             ......TVDSQGPTPVCTPTFLEKRKSEVAKM..NVDDKDN.EIVFKQPV...
MK67I_HUMAN/227-267              ......TVDSQGPTPVCTPTFLERRKSQVAEL..NDDDKDD.EIVFKQPI...
#=GR MK67I_HUMAN/227-267   SS    ......X--TTS------HHHHHHHHHHHHH-..-GGGGS-.SEEESS--...
G3RY58_GORGO/199-238             ...stk---GHGLTPVCTPTFLERRKSEVAEM..NDDDEAN.EIVFKQP-...
G5APY2_HETGA/132-171             ......TVDSQSATPVYTPTFLEKRKSEVAEM..NDD-KDD.EIVFKQPV...
G1LEM7_AILME/231-271             ......TVDSQGPTPVCTPTFLEKRKSEVAKM..NVDDKDN.EIVFKQPV...
B1MTB3_CALMO/226-266             ......TVDSQGPTPVCTPTFLERRKSEVAEM..NDDDKDN.EIVFKQPI...
F1RXX7_PIG/149-189               ......TVDSQGPTPVCTPTFLEKRKSEVAKM..NDDDKDH.EIVFKQPV...
G3N9P4_GASAC/227-264             ......SMCSDDVTPICTPSVLERRKSTVVD-..--DDGDD.EIILRMP-s..
F6XSZ6_MONDO/206-246             ......TVDSQGATPICSPTFLERRKAQAAEM..NDDDDDN.EIIFKLPP...
G1NH99_MELGA/230-268             ....cl--NSQDPTPVCTPTVLERRKAQQAD-..-DDTDDN.EIMIKLPP...
F7GRP8_MACMU/224-263             ......TVDTQGPTPVCTLTFLER-KSEVAEM..NDDDENN.EIVFKQPI...
G1P747_MYOLU/231-270             ......TVDSQGPTPVCTPTFLDKRKSEVAEM..NDD-EDN.EIVFKQP-l..
MK67I_RAT/206-243                ......SEANQDPTPVCTPTFLERRKSQLMEI..NDD---D.EIILKLPV...
G3UGN5_LOXAF/250-290             ......STNSQGSTPVCTPTFLERRKCEVAEM..NDDEEDK.EIIFKQPV...
H2PXL1_PONAB/192-232             ......TVDSQGPTPVCTPTFLERRKSEVAEL..NDDDKDD.EIVFKQPI...
G3WEC9_SARHA/256-296             ......SMDSQGPTPICSPTFLERRKAQAAEM..NDDDEDN.EIVLKLPP...
H9G0R4_MACMU/227-267             ......TVDSQGPTPVCTPTFLERRKSEVAEL..NDDDKDN.EIVFKQPI...
G3N9N9_GASAC/208-245             ......SMCSDDVTPICTPSVLERRKSTVVD-..--DDGDD.EIILRMP-s..
#=GC SS_cons                     ......X--TTS------HHHHHHHHHHHHH-..-GGGGS-.SEEESS--...
#=GC seq_cons                    ......oVDSQGsTPVCTPTFLERRKSpVA-h..NDDDcDs.EIVFKpP....
//
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