Feng L, Wang W, Cheng J, Ren Y, Zhao G, Gao C,
Tang Y, Liu X, Han W, Peng X, Liu R, Wang L.
Genome and proteome of long-chain alkane degrading Geobacillus
thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir.
Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5602-7. Epub 2007 Mar 19.
The complete genome sequence of Geobacillus thermodenitrificans NG80-2, a
thermophilic bacillus isolated from a deep oil reservoir in Northern China,
consists of a 3,550,319-bp chromosome and a 57,693-bp plasmid. The genome reveals
that NG80-2 is well equipped for adaptation into a wide variety of environmental
niches, including oil reservoirs, by possessing genes for utilization of a broad
range of energy sources, genes encoding various transporters for efficient
nutrient uptake and detoxification, and genes for a flexible respiration system
including an aerobic branch comprising five terminal oxidases and an anaerobic
branch comprising a complete denitrification pathway for quick response to
dissolved oxygen fluctuation. The identification of a nitrous oxide reductase
gene has not been previously described in Gram-positive bacteria. The proteome
further reveals the presence of a long-chain alkane degradation pathway; and the
function of the key enzyme in the pathway, the long-chain alkane monooxygenase
LadA, is confirmed by in vivo and in vitro experiments. The thermophilic soluble
monomeric LadA is an ideal candidate for treatment of environmental oil
pollutions and biosynthesis of complex molecules.
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