PMID:
     17372208
Authors:
     Feng L, Wang W, Cheng J, Ren Y, Zhao G, Gao C,
     Tang Y, Liu X, Han W, Peng X, Liu R, Wang L.
Title:
     Genome and proteome of long-chain alkane degrading Geobacillus
     thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir.
Journal:
     Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5602-7. Epub 2007 Mar 19.
Abstract:
     The complete genome sequence of Geobacillus thermodenitrificans NG80-2, a
     thermophilic bacillus isolated from a deep oil reservoir in Northern China,
     consists of a 3,550,319-bp chromosome and a 57,693-bp plasmid. The genome reveals
     that NG80-2 is well equipped for adaptation into a wide variety of environmental 
     niches, including oil reservoirs, by possessing genes for utilization of a broad 
     range of energy sources, genes encoding various transporters for efficient
     nutrient uptake and detoxification, and genes for a flexible respiration system
     including an aerobic branch comprising five terminal oxidases and an anaerobic
     branch comprising a complete denitrification pathway for quick response to
     dissolved oxygen fluctuation. The identification of a nitrous oxide reductase
     gene has not been previously described in Gram-positive bacteria. The proteome
     further reveals the presence of a long-chain alkane degradation pathway; and the 
     function of the key enzyme in the pathway, the long-chain alkane monooxygenase
     LadA, is confirmed by in vivo and in vitro experiments. The thermophilic soluble 
     monomeric LadA is an ideal candidate for treatment of environmental oil
     pollutions and biosynthesis of complex molecules.

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