LOCUS NC_000913 4639675 bp DNA circular BCT 04-MAR-2013
DEFINITION Escherichia coli str. K-12 substr. MG1655, complete genome.
ACCESSION NC_000913
VERSION NC_000913.2 GI:49175990
DBLINK Project: 57779
BioProject: PRJNA57779
KEYWORDS .
SOURCE Escherichia coli str. K-12 substr. MG1655
ORGANISM Escherichia coli str. K-12 substr. MG1655
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
Enterobacteriaceae; Escherichia.
REFERENCE 1 (bases 1 to 4639675)
AUTHORS Riley,M., Abe,T., Arnaud,M.B., Berlyn,M.K., Blattner,F.R.,
Chaudhuri,R.R., Glasner,J.D., Horiuchi,T., Keseler,I.M., Kosuge,T.,
Mori,H., Perna,N.T., Plunkett,G. III, Rudd,K.E., Serres,M.H.,
Thomas,G.H., Thomson,N.R., Wishart,D. and Wanner,B.L.
TITLE Escherichia coli K-12: a cooperatively developed annotation
snapshot--2005
JOURNAL Nucleic Acids Res. 34 (1), 1-9 (2006)
PUBMED 16397293
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 4639675)
AUTHORS Blattner,F.R., Plunkett,G. III, Bloch,C.A., Perna,N.T., Burland,V.,
Riley,M., Collado-Vides,J., Glasner,J.D., Rode,C.K., Mayhew,G.F.,
Gregor,J., Davis,N.W., Kirkpatrick,H.A., Goeden,M.A., Rose,D.J.,
Mau,B. and Shao,Y.
TITLE The complete genome sequence of Escherichia coli K-12
JOURNAL Science 277 (5331), 1453-1474 (1997)
PUBMED 9278503
REFERENCE 3 (bases 1 to 4639675)
AUTHORS Arnaud,M., Berlyn,M.K.B., Blattner,F.R., Galperin,M.Y.,
Glasner,J.D., Horiuchi,T., Kosuge,T., Mori,H., Perna,N.T.,
Plunkett,G. III, Riley,M., Rudd,K.E., Serres,M.H., Thomas,G.H. and
Wanner,B.L.
TITLE Workshop on Annotation of Escherichia coli K-12
JOURNAL Unpublished
REMARK Woods Hole, Mass., on 14-18 November 2003 (sequence corrections)
REFERENCE 4 (bases 1 to 4639675)
AUTHORS Glasner,J.D., Perna,N.T., Plunkett,G. III, Anderson,B.D.,
Bockhorst,J., Hu,J.C., Riley,M., Rudd,K.E. and Serres,M.H.
TITLE ASAP: Escherichia coli K-12 strain MG1655 version m56
JOURNAL Unpublished
REMARK ASAP download 10 June 2004 (annotation updates)
REFERENCE 5 (bases 1 to 4639675)
AUTHORS Hayashi,K., Morooka,N., Mori,H. and Horiuchi,T.
TITLE A more accurate sequence comparison between genomes of Escherichia
coli K12 W3110 and MG1655 strains
JOURNAL Unpublished
REMARK GenBank accessions AG613214 to AG613378 (sequence corrections)
REFERENCE 6 (bases 1 to 4639675)
AUTHORS Perna,N.T.
TITLE Escherichia coli K-12 MG1655 yqiK-rfaE intergenic region, genomic
sequence correction
JOURNAL Unpublished
REMARK GenBank accession AY605712 (sequence corrections)
REFERENCE 7 (bases 1 to 4639675)
AUTHORS Rudd,K.E.
TITLE A manual approach to accurate translation start site annotation: an
E. coli K-12 case study
JOURNAL Unpublished
REFERENCE 8 (bases 1 to 4639675)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (04-MAR-2013) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 9 (bases 1 to 4639675)
AUTHORS Rudd,K.E.
TITLE Direct Submission
JOURNAL Submitted (06-FEB-2013) Department of Biochemistry and Molecular
Biology, University of Miami Miller School of Medicine, 118 Gautier
Bldg., Miami, FL 33136, USA
REMARK Sequence update by submitter
REFERENCE 10 (bases 1 to 4639675)
AUTHORS Rudd,K.E.
TITLE Direct Submission
JOURNAL Submitted (24-APR-2007) Department of Biochemistry and Molecular
Biology, University of Miami Miller School of Medicine, 118 Gautier
Bldg., Miami, FL 33136, USA
REMARK Annotation update from ecogene.org as a multi-database
collaboration
REFERENCE 11 (bases 1 to 4639675)
AUTHORS Plunkett,G. III.
TITLE Direct Submission
JOURNAL Submitted (07-FEB-2006) Laboratory of Genetics, University of
Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
REMARK Protein updates by submitter
REFERENCE 12 (bases 1 to 4639675)
AUTHORS Plunkett,G. III.
TITLE Direct Submission
JOURNAL Submitted (10-JUN-2004) Laboratory of Genetics, University of
Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
REMARK Sequence update by submitter
REFERENCE 13 (bases 1 to 4639675)
AUTHORS Plunkett,G. III.
TITLE Direct Submission
JOURNAL Submitted (13-OCT-1998) Laboratory of Genetics, University of
Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
REFERENCE 14 (bases 1 to 4639675)
AUTHORS Blattner,F.R. and Plunkett,G. III.
TITLE Direct Submission
JOURNAL Submitted (02-SEP-1997) Laboratory of Genetics, University of
Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
REFERENCE 15 (bases 1 to 4639675)
AUTHORS Blattner,F.R. and Plunkett,G. III.
TITLE Direct Submission
JOURNAL Submitted (16-JAN-1997) Laboratory of Genetics, University of
Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence is identical to U00096.
On Jun 24, 2004 this sequence version replaced gi:16127994.
Current U00096 annotation updates are derived from EcoGene
http://ecogene.org. Suggestions for updates can be sent to Dr.
Kenneth Rudd (krudd@miami.edu). These updates are being generated
from a collaboration that also includes ASAP/ERIC, the Coli Genetic
Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..4639675
/organism="Escherichia coli str. K-12 substr. MG1655"
/mol_type="genomic DNA"
/strain="K-12"
/sub_strain="MG1655"
/db_xref="taxon:511145"
gene 190..255
/gene="thrL"
/locus_tag="b0001"
/gene_synonym="ECK0001; JW4367"
/db_xref="EcoGene:EG11277"
/db_xref="GeneID:944742"
CDS 190..255
/gene="thrL"
/locus_tag="b0001"
/gene_synonym="ECK0001; JW4367"
/function="leader; Amino acid biosynthesis: Threonine"
/function="1.5.1.8 metabolism; building block
biosynthesis; amino acids; threonine"
/GO_process="GO:0009088 - threonine biosynthetic process"
/codon_start=1
/transl_table=11
/product="thr operon leader peptide"
/protein_id="NP_414542.1"
/db_xref="GI:16127995"
/db_xref="ASAP:ABE-0000006"
/db_xref="UniProtKB/Swiss-Prot:P0AD86"
/db_xref="EcoGene:EG11277"
/db_xref="GeneID:944742"
/translation="MKRISTTITTTITITTGNGAG"
gene 337..2799
/gene="thrA"
/locus_tag="b0002"
/gene_synonym="ECK0002; Hs; JW0001; thrA1; thrA2; thrD"
/db_xref="EcoGene:EG10998"
/db_xref="GeneID:945803"
CDS 337..2799
/gene="thrA"
/locus_tag="b0002"
/gene_synonym="ECK0002; Hs; JW0001; thrA1; thrA2; thrD"
/EC_number="1.1.1.3"
/EC_number="2.7.2.4"
/function="enzyme; Amino acid biosynthesis: Threonine"
/function="1.5.1.8 metabolism; building block
biosynthesis; amino acids; threonine"
/function="1.5.1.21 metabolism; building block
biosynthesis; amino acids; homoserine"
/function="7.1 location of gene products; cytoplasm"
/experiment="N-terminus verified by Edman degradation:
PMID 354697,4562989"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0009088 - threonine biosynthetic process"
/GO_process="GO:0009086 - methionine biosynthetic process"
/GO_process="GO:0009090 - homoserine biosynthetic process"
/note="bifunctional: aspartokinase I (N-terminal);
homoserine dehydrogenase I (C-terminal)"
/codon_start=1
/transl_table=11
/product="fused aspartokinase I and homoserine
dehydrogenase I"
/protein_id="NP_414543.1"
/db_xref="GI:16127996"
/db_xref="ASAP:ABE-0000008"
/db_xref="UniProtKB/Swiss-Prot:P00561"
/db_xref="EcoGene:EG10998"
/db_xref="GeneID:945803"
/translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
NDVTAAGVFADLLRTLSWKLGV"
misc_feature 337..2796
/gene="thrA"
/locus_tag="b0002"
/gene_synonym="ECK0002; Hs; JW0001; thrA1; thrA2; thrD"
/note="bifunctional aspartokinase I/homoserine
dehydrogenase I; Provisional; Region: thrA; PRK09436"
/db_xref="CDD:181856"
misc_feature 337..1224
/gene="thrA"
/locus_tag="b0002"
/gene_synonym="ECK0002; Hs; JW0001; thrA1; thrA2; thrD"
/note="AAK_AK-HSDH: Amino Acid Kinase Superfamily (AAK),
AK-HSDH; this CD includes the N-terminal catalytic domain
of aspartokinase (AK) of the bifunctional enzyme AK -
homoserine dehydrogenase (HSDH). These aspartokinases are
found in bacteria (E. coli...; Region: AAK_AK-HSDH;
cd04257"
/db_xref="CDD:239790"
misc_feature order(349..351,466..468,715..717,958..960,1093..1095,
1132..1134)
/gene="thrA"
/locus_tag="b0002"
/gene_synonym="ECK0002; Hs; JW0001; thrA1; thrA2; thrD"
/note="putative catalytic residues [active]"
/db_xref="CDD:239790"
misc_feature order(349..351,355..363,1018..1023,1030..1035)
/gene="thrA"
/locus_tag="b0002"
/gene_synonym="ECK0002; Hs; JW0001; thrA1; thrA2; thrD"
/note="putative nucleotide binding site [chemical
binding]; other site"
/db_xref="CDD:239790"
misc_feature order(448..453,466..468,715..717)
/gene="thrA"
/locus_tag="b0002"
/gene_synonym="ECK0002; Hs; JW0001; thrA1; thrA2; thrD"
/note="putative aspartate binding site [chemical binding];
other site"
/db_xref="CDD:239790"
misc_feature 1279..1518
/gene="thrA"
/locus_tag="b0002"
/gene_synonym="ECK0002; Hs; JW0001; thrA1; thrA2; thrD"
/note="ACT domains of the bifunctional enzyme
aspartokinase (AK) - homoserine dehydrogenase (HSDH);
Region: ACT_AKi-HSDH-ThrA-like_1; cd04921"
/db_xref="CDD:153193"
misc_feature order(1303..1311,1318..1323,1333..1335,1342..1347,
1366..1368,1372..1392,1396..1401,1405..1407,1411..1413)
/gene="thrA"
/locus_tag="b0002"
/gene_synonym="ECK0002; Hs; JW0001; thrA1; thrA2; thrD"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:153193"
misc_feature 1387..1389
/gene="thrA"
/locus_tag="b0002"
/gene_synonym="ECK0002; Hs; JW0001; thrA1; thrA2; thrD"
/note="putative threonine allosteric regulatory site;
other site"
/db_xref="CDD:153193"
misc_feature 1522..1719
/gene="thrA"
/locus_tag="b0002"
/gene_synonym="ECK0002; Hs; JW0001; thrA1; thrA2; thrD"
/note="ACT domains of the bifunctional enzyme
aspartokinase (AK) - homoserine dehydrogenase (HSDH);
Region: ACT_AKi-HSDH-ThrA_2; cd04922"
/db_xref="CDD:153194"
misc_feature 1630..1632
/gene="thrA"
/locus_tag="b0002"
/gene_synonym="ECK0002; Hs; JW0001; thrA1; thrA2; thrD"
/note="putative threonine allosteric regulatory site;
other site"
/db_xref="CDD:153194"
misc_feature 1771..2151
/gene="thrA"
/locus_tag="b0002"
/gene_synonym="ECK0002; Hs; JW0001; thrA1; thrA2; thrD"
/note="Homoserine dehydrogenase, NAD binding domain;
Region: NAD_binding_3; pfam03447"
/db_xref="CDD:217564"
misc_feature 2176..2766
/gene="thrA"
/locus_tag="b0002"
/gene_synonym="ECK0002; Hs; JW0001; thrA1; thrA2; thrD"
/note="Homoserine dehydrogenase; Region: Homoserine_dh;
pfam00742"
/db_xref="CDD:216092"
gene 2801..3733
/gene="thrB"
/locus_tag="b0003"
/gene_synonym="ECK0003; JW0002"
/db_xref="EcoGene:EG10999"
/db_xref="GeneID:947498"
CDS 2801..3733
/gene="thrB"
/locus_tag="b0003"
/gene_synonym="ECK0003; JW0002"
/EC_number="2.7.1.39"
/function="enzyme; Amino acid biosynthesis: Threonine"
/function="1.5.1.8 metabolism; building block
biosynthesis; amino acids; threonine"
/function="7.1 location of gene products; cytoplasm"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0009088 - threonine biosynthetic process"
/codon_start=1
/transl_table=11
/product="homoserine kinase"
/protein_id="NP_414544.1"
/db_xref="GI:16127997"
/db_xref="ASAP:ABE-0000010"
/db_xref="UniProtKB/Swiss-Prot:P00547"
/db_xref="EcoGene:EG10999"
/db_xref="GeneID:947498"
/translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPETA
QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
misc_feature 2804..3724
/gene="thrB"
/locus_tag="b0003"
/gene_synonym="ECK0003; JW0002"
/note="homoserine kinase; Region: thrB; TIGR00191"
/db_xref="CDD:129295"
misc_feature <2810..3160
/gene="thrB"
/locus_tag="b0003"
/gene_synonym="ECK0003; JW0002"
/note="Protein of unknown function; Region: YhfT;
pfam10797"
/db_xref="CDD:220877"
misc_feature 3050..3250
/gene="thrB"
/locus_tag="b0003"
/gene_synonym="ECK0003; JW0002"
/note="GHMP kinases N terminal domain; Region:
GHMP_kinases_N; pfam00288"
/db_xref="CDD:215839"
gene 3734..5020
/gene="thrC"
/locus_tag="b0004"
/gene_synonym="ECK0004; JW0003"
/db_xref="EcoGene:EG11000"
/db_xref="GeneID:945198"
CDS 3734..5020
/gene="thrC"
/locus_tag="b0004"
/gene_synonym="ECK0004; JW0003"
/EC_number="4.2.3.1"
/function="enzyme; Amino acid biosynthesis: Threonine"
/function="1.5.1.8 metabolism; building block
biosynthesis; amino acids; threonine"
/function="7.1 location of gene products; cytoplasm"
/experiment="N-terminus verified by Edman degradation:
PMID 9298646,9600841,9740056"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0009088 - threonine biosynthetic process"
/codon_start=1
/transl_table=11
/product="threonine synthase"
/protein_id="NP_414545.1"
/db_xref="GI:16127998"
/db_xref="ASAP:ABE-0000012"
/db_xref="UniProtKB/Swiss-Prot:P00934"
/db_xref="EcoGene:EG11000"
/db_xref="GeneID:945198"
/translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
LLAQICYYFEAVAQLPQETRNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
misc_feature 3734..5014
/gene="thrC"
/locus_tag="b0004"
/gene_synonym="ECK0004; JW0003"
/note="Threonine synthase [Amino acid transport and
metabolism]; Region: ThrC; COG0498"
/db_xref="CDD:223572"
misc_feature 3737..5002
/gene="thrC"
/locus_tag="b0004"
/gene_synonym="ECK0004; JW0003"
/note="Threonine synthase catalyzes the final step of
threonine biosynthesis. The conversion of
O-phosphohomoserine into threonine and inorganic phosphate
is pyridoxal 5'-phosphate dependent. The Thr-synth_1 CD
includes members from higher plants; Region: Thr-synth_2;
cd01560"
/db_xref="CDD:107203"
misc_feature order(4052..4054,4475..4480,4859..4861)
/gene="thrC"
/locus_tag="b0004"
/gene_synonym="ECK0004; JW0003"
/note="pyridoxal 5'-phosphate binding site [chemical
binding]; other site"
/db_xref="CDD:107203"
misc_feature 4052..4054
/gene="thrC"
/locus_tag="b0004"
/gene_synonym="ECK0004; JW0003"
/note="catalytic residue [active]"
/db_xref="CDD:107203"
gene 5234..5530
/gene="yaaX"
/locus_tag="b0005"
/gene_synonym="ECK0005; JW0004"
/db_xref="EcoGene:EG14384"
/db_xref="GeneID:944747"
CDS 5234..5530
/gene="yaaX"
/locus_tag="b0005"
/gene_synonym="ECK0005; JW0004"
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="NP_414546.1"
/db_xref="GI:16127999"
/db_xref="ASAP:ABE-0000015"
/db_xref="UniProtKB/Swiss-Prot:P75616"
/db_xref="EcoGene:EG14384"
/db_xref="GeneID:944747"
/translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
YWDGGHWRDHGWWKQHYEWRGNRWHLHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
misc_feature 5306..>5437
/gene="yaaX"
/locus_tag="b0005"
/gene_synonym="ECK0005; JW0004"
/note="Protein of unknown function (DUF2502); Region:
DUF2502; pfam10697"
/db_xref="CDD:220851"
repeat_region 5565..5669
/note="RIP1 (repetitive extragenic palindromic) element;
contains 2 REP sequences and 1 IHF site"
gene complement(5683..6459)
/gene="yaaA"
/locus_tag="b0006"
/gene_synonym="ECK0006; JW0005"
/db_xref="EcoGene:EG10011"
/db_xref="GeneID:944749"
CDS complement(5683..6459)
/gene="yaaA"
/locus_tag="b0006"
/gene_synonym="ECK0006; JW0005"
/codon_start=1
/transl_table=11
/product="peroxide resistance protein, lowers
intracellular iron"
/protein_id="NP_414547.1"
/db_xref="GI:16128000"
/db_xref="ASAP:ABE-0000018"
/db_xref="UniProtKB/Swiss-Prot:P0A8I3"
/db_xref="EcoGene:EG10011"
/db_xref="GeneID:944749"
/translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
misc_feature complement(5689..6459)
/gene="yaaA"
/locus_tag="b0006"
/gene_synonym="ECK0006; JW0005"
/note="hypothetical protein; Validated; Region: PRK02101"
/db_xref="CDD:234999"
gene complement(6529..7959)
/gene="yaaJ"
/locus_tag="b0007"
/gene_synonym="ECK0007; JW0006"
/db_xref="EcoGene:EG11555"
/db_xref="GeneID:944745"
CDS complement(6529..7959)
/gene="yaaJ"
/locus_tag="b0007"
/gene_synonym="ECK0007; JW0006"
/function="putative transport; Transport of small
molecules: Amino acids, amines"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/note="inner membrane transport protein"
/codon_start=1
/transl_table=11
/product="putative transporter"
/protein_id="NP_414548.1"
/db_xref="GI:16128001"
/db_xref="ASAP:ABE-0000020"
/db_xref="UniProtKB/Swiss-Prot:P30143"
/db_xref="EcoGene:EG11555"
/db_xref="GeneID:944745"
/translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
VQANAVARALSFSFDFPPLVTGIILAVFTLLAITRGLHGVARLMQGFVPLMAIIWVLT
SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
PVFDPLRYPDIGRQLSPDAWDDVSQE"
misc_feature complement(6613..7959)
/gene="yaaJ"
/locus_tag="b0007"
/gene_synonym="ECK0007; JW0006"
/note="Na+/alanine symporter [Amino acid transport and
metabolism]; Region: AlsT; COG1115"
/db_xref="CDD:224040"
misc_feature complement(6586..7842)
/gene="yaaJ"
/locus_tag="b0007"
/gene_synonym="ECK0007; JW0006"
/note="Sodium:alanine symporter family; Region:
Na_Ala_symp; pfam01235"
/db_xref="CDD:216380"
gene 8238..9191
/gene="talB"
/locus_tag="b0008"
/gene_synonym="ECK0008; JW0007; yaaK"
/db_xref="EcoGene:EG11556"
/db_xref="GeneID:944748"
CDS 8238..9191
/gene="talB"
/locus_tag="b0008"
/gene_synonym="ECK0008; JW0007; yaaK"
/EC_number="2.2.1.2"
/function="enzyme; Central intermediary metabolism:
Non-oxidative branch, pentose pathway"
/function="1.7.3 metabolism; central intermediary
metabolism; pentose phosphate shunt, non-oxidative branch"
/function="7.1 location of gene products; cytoplasm"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0009052 - pentose-phosphate shunt,
non-oxidative branch"
/codon_start=1
/transl_table=11
/product="transaldolase B"
/protein_id="NP_414549.1"
/db_xref="GI:16128002"
/db_xref="ASAP:ABE-0000027"
/db_xref="UniProtKB/Swiss-Prot:P0A870"
/db_xref="EcoGene:EG11556"
/db_xref="GeneID:944748"
/translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
misc_feature 8238..9188
/gene="talB"
/locus_tag="b0008"
/gene_synonym="ECK0008; JW0007; yaaK"
/note="transaldolase-like protein; Provisional; Region:
PTZ00411"
/db_xref="CDD:240406"
mat_peptide 8241..9188
/gene="talB"
/locus_tag="b0008"
/gene_synonym="ECK0008; JW0007; yaaK"
/product="transaldolase B"
/experiment="N-terminus verified by Edman degradation:
PMID 8740179,9298646"
misc_feature 8247..9176
/gene="talB"
/locus_tag="b0008"
/gene_synonym="ECK0008; JW0007; yaaK"
/note="Transaldolases including both TalA and TalB;
Region: Transaldolase_TalAB; cd00957"
/db_xref="CDD:188644"
misc_feature order(8286..8288,8334..8336,8340..8345,8517..8519,
8523..8525,8631..8633,8697..8699,8703..8705,8763..8765,
8778..8780)
/gene="talB"
/locus_tag="b0008"
/gene_synonym="ECK0008; JW0007; yaaK"
/note="active site"
/db_xref="CDD:188644"
misc_feature order(8547..8549,9072..9074,9081..9083,9093..9098,
9114..9116,9123..9125,9135..9137)
/gene="talB"
/locus_tag="b0008"
/gene_synonym="ECK0008; JW0007; yaaK"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:188644"
misc_feature 8631..8633
/gene="talB"
/locus_tag="b0008"
/gene_synonym="ECK0008; JW0007; yaaK"
/note="catalytic residue [active]"
/db_xref="CDD:188644"
gene 9306..9893
/gene="mog"
/locus_tag="b0009"
/gene_synonym="bisD; chlG; ECK0009; JW0008; mogA; yaaG"
/db_xref="EcoGene:EG11511"
/db_xref="GeneID:944760"
CDS 9306..9893
/gene="mog"
/locus_tag="b0009"
/gene_synonym="bisD; chlG; ECK0009; JW0008; mogA; yaaG"
/function="transport; Biosynthesis of cofactors, carriers:
Molybdopterin"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006777 - Mo-molybdopterin cofactor
biosynthetic process"
/note="putative molybdochetalase in molybdopterine
biosynthesis"
/codon_start=1
/transl_table=11
/product="molybdochelatase incorporating molybdenum into
molybdopterin"
/protein_id="NP_414550.1"
/db_xref="GI:16128003"
/db_xref="ASAP:ABE-0000030"
/db_xref="UniProtKB/Swiss-Prot:P0AF03"
/db_xref="EcoGene:EG11511"
/db_xref="GeneID:944760"
/translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
misc_feature 9315..9773
/gene="mog"
/locus_tag="b0009"
/gene_synonym="bisD; chlG; ECK0009; JW0008; mogA; yaaG"
/note="MogA_MoaB family. Members of this family are
involved in biosynthesis of the molybdenum cofactor (MoCF)
an essential cofactor of a diverse group of redox enzymes.
MoCF biosynthesis is an evolutionarily conserved pathway
present in eubacteria, archaea; Region: MogA_MoaB;
cd00886"
/db_xref="CDD:238451"
misc_feature order(9528..9536,9624..9626,9702..9707,9717..9719,
9726..9728)
/gene="mog"
/locus_tag="b0009"
/gene_synonym="bisD; chlG; ECK0009; JW0008; mogA; yaaG"
/note="MPT binding site; other site"
/db_xref="CDD:238451"
misc_feature order(9534..9536,9540..9545,9549..9551,9561..9563,
9585..9593,9606..9608,9615..9620,9648..9650,9657..9659)
/gene="mog"
/locus_tag="b0009"
/gene_synonym="bisD; chlG; ECK0009; JW0008; mogA; yaaG"
/note="trimer interface [polypeptide binding]; other site"
/db_xref="CDD:238451"
gene complement(9928..10494)
/gene="yaaH"
/locus_tag="b0010"
/gene_synonym="ECK0010; JW0009"
/db_xref="EcoGene:EG11512"
/db_xref="GeneID:944792"
CDS complement(9928..10494)
/gene="yaaH"
/locus_tag="b0010"
/gene_synonym="ECK0010; JW0009"
/codon_start=1
/transl_table=11
/product="inner membrane protein, Grp1_Fun34_YaaH family"
/protein_id="NP_414551.1"
/db_xref="GI:16128004"
/db_xref="ASAP:ABE-0000032"
/db_xref="UniProtKB/Swiss-Prot:P0AC98"
/db_xref="EcoGene:EG11512"
/db_xref="GeneID:944792"
/translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
GASAIYLAMGEVLNEQFGRTVLPIGESH"
misc_feature complement(9931..10494)
/gene="yaaH"
/locus_tag="b0010"
/gene_synonym="ECK0010; JW0009"
/note="hypothetical protein; Provisional; Region:
PRK10659"
/db_xref="CDD:182625"
gene complement(10643..11356)
/gene="yaaW"
/locus_tag="b0011"
/gene_synonym="ECK0011; htgA; htpY; JW0010"
/db_xref="EcoGene:EG14340"
/db_xref="GeneID:944771"
CDS complement(10643..11356)
/gene="yaaW"
/locus_tag="b0011"
/gene_synonym="ECK0011; htgA; htpY; JW0010"
/function="putative enzyme; Not classified"
/codon_start=1
/transl_table=11
/product="conserved protein, UPF0174 family"
/protein_id="NP_414552.1"
/db_xref="GI:16128005"
/db_xref="ASAP:ABE-0000037"
/db_xref="UniProtKB/Swiss-Prot:P75617"
/db_xref="EcoGene:EG14340"
/db_xref="GeneID:944771"
/translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
IPAVLQIACLRRMVSATQV"
misc_feature complement(10646..11356)
/gene="yaaW"
/locus_tag="b0011"
/gene_synonym="ECK0011; htgA; htpY; JW0010"
/note="hypothetical protein; Provisional; Region:
PRK10236"
/db_xref="CDD:182323"
misc_feature complement(11240..11344)
/gene="yaaW"
/locus_tag="b0011"
/gene_synonym="ECK0011; htgA; htpY; JW0010"
/note="Domain of unknown function (DUF3944); Region:
DUF3944; pfam13099"
/db_xref="CDD:205280"
misc_feature complement(10646..11242)
/gene="yaaW"
/locus_tag="b0011"
/gene_synonym="ECK0011; htgA; htpY; JW0010"
/note="Uncharacterized protein conserved in bacteria
[Function unknown]; Region: COG4735"
/db_xref="CDD:227078"
gene complement(11382..11786)
/gene="yaaI"
/locus_tag="b0013"
/gene_synonym="ECK0013; JW0012"
/db_xref="EcoGene:EG11513"
/db_xref="GeneID:944751"
CDS complement(11382..11786)
/gene="yaaI"
/locus_tag="b0013"
/gene_synonym="ECK0013; JW0012"
/codon_start=1
/transl_table=11
/product="conserved protein, UPF0412 family"
/protein_id="NP_414554.1"
/db_xref="GI:16128007"
/db_xref="ASAP:ABE-0000043"
/db_xref="UniProtKB/Swiss-Prot:P28696"
/db_xref="EcoGene:EG11513"
/db_xref="GeneID:944751"
/translation="MKSVFTISASLAISLMLCCTAQANDHKLLGAIAMPRNETNDLAL
KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
misc_feature complement(11385..11786)
/gene="yaaI"
/locus_tag="b0013"
/gene_synonym="ECK0013; JW0012"
/note="hypothetical protein; Provisional; Region:
PRK10154"
/db_xref="CDD:182272"
gene 12163..14079
/gene="dnaK"
/locus_tag="b0014"
/gene_synonym="ECK0014; groPAB; groPC; groPF; grpC; grpF;
JW0013; seg"
/db_xref="EcoGene:EG10241"
/db_xref="GeneID:944750"
CDS 12163..14079
/gene="dnaK"
/locus_tag="b0014"
/gene_synonym="ECK0014; groPAB; groPC; groPF; grpC; grpF;
JW0013; seg"
/function="factor; Chaperones"
/function="2.3.4 information transfer; protein related;
chaperoning, folding"
/function="5.5.1 cell processes; adaptation to stress;
osmotic pressure"
/function="7.1 location of gene products; cytoplasm"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006457 - protein folding"
/GO_process="GO:0006970 - response to osmotic stress"
/note="chaperone Hsp70; DNA biosynthesis; autoregulated
heat shock proteins"
/codon_start=1
/transl_table=11
/product="chaperone Hsp70, co-chaperone with DnaJ"
/protein_id="NP_414555.1"
/db_xref="GI:16128008"
/db_xref="ASAP:ABE-0000052"
/db_xref="UniProtKB/Swiss-Prot:P0A6Y8"
/db_xref="EcoGene:EG10241"
/db_xref="GeneID:944750"
/translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
DVVDAEFEEVKDKK"
misc_feature 12163..13962
/gene="dnaK"
/locus_tag="b0014"
/gene_synonym="ECK0014; groPAB; groPC; groPF; grpC; grpF;
JW0013; seg"
/note="molecular chaperone DnaK; Provisional; Region:
dnaK; PRK00290"
/db_xref="CDD:234715"
misc_feature 12163..13269
/gene="dnaK"
/locus_tag="b0014"
/gene_synonym="ECK0014; groPAB; groPC; groPF; grpC; grpF;
JW0013; seg"
/note="Nucleotide-binding domain of human HSPA9,
Escherichia coli DnaK, and similar proteins; Region:
HSPA9-like_NBD; cd11733"
/db_xref="CDD:212683"
mat_peptide 12166..14076
/gene="dnaK"
/locus_tag="b0014"
/gene_synonym="ECK0014; groPAB; groPC; groPF; grpC; grpF;
JW0013; seg"
/product="chaperone Hsp70, co-chaperone with DnaJ"
/experiment="N-terminus verified by Edman degradation:
PMID 1396676,7783627,9298646"
misc_feature order(12190..12201,12370..12372,12673..12675,12748..12753,
12757..12759,12847..12849,12961..12963,12970..12972,
12982..12984,13183..13191,13195..13197)
/gene="dnaK"
/locus_tag="b0014"
/gene_synonym="ECK0014; groPAB; groPC; groPF; grpC; grpF;
JW0013; seg"
/note="nucleotide binding site [chemical binding]; other
site"
/db_xref="CDD:212683"
misc_feature order(12244..12246,12253..12255,12307..12309,12319..12321,
12328..12333,12337..12342,12547..12558,12931..12933,
12940..12945,12952..12954,13006..13008,13012..13017)
/gene="dnaK"
/locus_tag="b0014"
/gene_synonym="ECK0014; groPAB; groPC; groPF; grpC; grpF;
JW0013; seg"
/note="NEF interaction site [polypeptide binding]; other
site"
/db_xref="CDD:212683"
misc_feature order(12604..12609,12613..12618,12661..12666,12670..12672,
12808..12819)
/gene="dnaK"
/locus_tag="b0014"
/gene_synonym="ECK0014; groPAB; groPC; groPF; grpC; grpF;
JW0013; seg"
/note="SBD interface [polypeptide binding]; other site"
/db_xref="CDD:212683"
gene 14168..15298
/gene="dnaJ"
/locus_tag="b0015"
/gene_synonym="ECK0015; faa; groP; grpC; JW0014"
/db_xref="EcoGene:EG10240"
/db_xref="GeneID:944753"
CDS 14168..15298
/gene="dnaJ"
/locus_tag="b0015"
/gene_synonym="ECK0015; faa; groP; grpC; JW0014"
/function="factor; Chaperones"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006457 - protein folding"
/note="chaperone with DnaK; heat shock protein"
/codon_start=1
/transl_table=11
/product="chaperone Hsp40, co-chaperone with DnaK"
/protein_id="NP_414556.1"
/db_xref="GI:16128009"
/db_xref="ASAP:ABE-0000054"
/db_xref="UniProtKB/Swiss-Prot:P08622"
/db_xref="EcoGene:EG10240"
/db_xref="GeneID:944753"
/translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
ERQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
misc_feature 14168..15283
/gene="dnaJ"
/locus_tag="b0015"
/gene_synonym="ECK0015; faa; groP; grpC; JW0014"
/note="chaperone protein DnaJ; Provisional; Region:
PRK10767"
/db_xref="CDD:236757"
mat_peptide 14171..15295
/gene="dnaJ"
/locus_tag="b0015"
/gene_synonym="ECK0015; faa; groP; grpC; JW0014"
/product="chaperone Hsp40, co-chaperone with DnaK"
/experiment="N-terminus verified by Edman degradation:
PMID 3003084"
misc_feature 14180..14344
/gene="dnaJ"
/locus_tag="b0015"
/gene_synonym="ECK0015; faa; groP; grpC; JW0014"
/note="DnaJ domain or J-domain. DnaJ/Hsp40 (heat shock
protein 40) proteins are highly conserved and play crucial
roles in protein translation, folding, unfolding,
translocation, and degradation. They act primarily by
stimulating the ATPase activity of Hsp70s; Region: DnaJ;
cd06257"
/db_xref="CDD:99751"
misc_feature order(14264..14272,14297..14299,14306..14311,14318..14323)
/gene="dnaJ"
/locus_tag="b0015"
/gene_synonym="ECK0015; faa; groP; grpC; JW0014"
/note="HSP70 interaction site [polypeptide binding]; other
site"
/db_xref="CDD:99751"
misc_feature 14513..>14596
/gene="dnaJ"
/locus_tag="b0015"
/gene_synonym="ECK0015; faa; groP; grpC; JW0014"
/note="C-terminal substrate binding domain of DnaJ and
HSP40; Region: DnaJ_C; cl03262"
/db_xref="CDD:243362"
misc_feature order(14522..14524,14567..14584)
/gene="dnaJ"
/locus_tag="b0015"
/gene_synonym="ECK0015; faa; groP; grpC; JW0014"
/note="substrate binding site [polypeptide binding]; other
site"
/db_xref="CDD:199909"
misc_feature 14597..14779
/gene="dnaJ"
/locus_tag="b0015"
/gene_synonym="ECK0015; faa; groP; grpC; JW0014"
/note="Zinc finger domain of DnaJ and HSP40; Region:
DnaJ_zf; cd10719"
/db_xref="CDD:199908"
misc_feature order(14597..14599,14606..14608,14648..14650,14657..14659,
14714..14716,14723..14725,14756..14758,14765..14767)
/gene="dnaJ"
/locus_tag="b0015"
/gene_synonym="ECK0015; faa; groP; grpC; JW0014"
/note="Zn binding sites [ion binding]; other site"
/db_xref="CDD:199908"
misc_feature 14780..15157
/gene="dnaJ"
/locus_tag="b0015"
/gene_synonym="ECK0015; faa; groP; grpC; JW0014"
/note="C-terminal substrate binding domain of DnaJ and
HSP40; Region: DnaJ_C; cd10747"
/db_xref="CDD:199909"
misc_feature order(14978..14983,14990..14995,15053..15064,15152..15157)
/gene="dnaJ"
/locus_tag="b0015"
/gene_synonym="ECK0015; faa; groP; grpC; JW0014"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:199909"
mobile_element 15387..16731
/mobile_element_type="insertion sequence:IS186A"
gene 15445..16557
/gene="insL1"
/locus_tag="b0016"
/gene_synonym="ECK0016"
/db_xref="EcoGene:EG40012"
/db_xref="GeneID:944754"
CDS 15445..16557
/gene="insL1"
/locus_tag="b0016"
/gene_synonym="ECK0016"
/function="IS, phage, Tn; Transposon-related functions;
extrachromosomal; transposon related"
/codon_start=1
/transl_table=11
/product="IS186 transposase"
/protein_id="NP_414557.1"
/db_xref="GI:16128010"
/db_xref="ASAP:ABE-0000058"
/db_xref="UniProtKB/Swiss-Prot:P0CF91"
/db_xref="EcoGene:EG40012"
/db_xref="GeneID:944754"
/translation="MNYSHDNWSAILAHIGKPEELDTSARNAGALTRRREIRDAATLL
RLGLAYGPGGMSLREVTAWAQLHDVATLSDVALLKRLRNAADWFGILAAQTLAVRAAV
TGCTSGKRLRLVDGTAISAPGGGSAEWRLHMGYDPHTCQFTDFELTDSRDAERLDRFA
QTADEIRIADRGFGSRPECIRSLAFGEADYIVRVHWRGLRWLTAEGMRFDMMGFLRGL
DCGKNGETTVMIGNSGNKKAGAPFPARLIAVSLPPEKALISKTRLLSENRRKGRVVQA
ETLEAAGHVLLLTSLPEDEYSAEQVADCYRLRWQIELAFKRLKSLLHLDALRAKEPEL
AKAWIFANLLAAFLIDDIIQPSLDFPPRSAGSEKKN"
misc_feature 15763..16488
/gene="insL1"
/locus_tag="b0016"
/gene_synonym="ECK0016"
/note="Transposase DDE domain; Region: DDE_Tnp_1;
pfam01609"
/db_xref="CDD:216602"
misc_feature <16162..16491
/gene="insL1"
/locus_tag="b0016"
/gene_synonym="ECK0016"
/note="FOG: Transposase and inactivated derivatives [DNA
replication, recombination, and repair]; Region: COG3385"
/db_xref="CDD:225920"
gene complement(16751..16960)
/gene="mokC"
/locus_tag="b0018"
/gene_synonym="ECK4466; gefL"
/db_xref="EcoGene:EG12074"
/db_xref="GeneID:944756"
CDS complement(16751..16960)
/gene="mokC"
/locus_tag="b0018"
/gene_synonym="ECK4466; gefL"
/function="component of addiction module"
/function="modulation of cell killing"
/function="5.6.3 cell processes; protection; cell killing"
/function="10 cryptic genes"
/note="regulatory peptide whose translation enables hokC
(gef) expression"
/codon_start=1
/transl_table=11
/product="regulatory protein for HokC, overlaps CDS of
hokC"
/protein_id="NP_414559.1"
/db_xref="GI:16128012"
/db_xref="ASAP:ABE-0000064"
/db_xref="UniProtKB/Swiss-Prot:P33236"
/db_xref="EcoGene:EG12074"
/db_xref="GeneID:944756"
/translation="MLNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVT
RKDLCEVHIRTGQTEVAVFTAYESE"
misc_feature complement(16763..16891)
/gene="mokC"
/locus_tag="b0018"
/gene_synonym="ECK4466; gefL"
/note="Hok/gef family; Region: HOK_GEF; pfam01848"
/db_xref="CDD:202008"
gene complement(16751..16903)
/gene="hokC"
/locus_tag="b4412"
/gene_synonym="ECK0018; gef; JW5879"
/db_xref="EcoGene:EG10373"
/db_xref="GeneID:2847744"
CDS complement(16751..16903)
/gene="hokC"
/locus_tag="b4412"
/gene_synonym="ECK0018; gef; JW5879"
/function="component of addiction module"
/function="cell killing"
/note="small toxic membrane polypeptide"
/codon_start=1
/transl_table=11
/product="toxic membrane protein, small"
/protein_id="YP_025292.1"
/db_xref="GI:49175991"
/db_xref="ASAP:ABE-0047278"
/db_xref="UniProtKB/Swiss-Prot:P0ACG4"
/db_xref="EcoGene:EG10373"
/db_xref="GeneID:2847744"
/translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
TAYESE"
misc_feature complement(16763..16891)
/gene="hokC"
/locus_tag="b4412"
/gene_synonym="ECK0018; gef; JW5879"
/note="Hok/gef family; Region: HOK_GEF; pfam01848"
/db_xref="CDD:202008"
gene 16952..17006
/gene="sokC"
/locus_tag="b4413"
/gene_synonym="ECK0019; JWR0224; sof"
/db_xref="EcoGene:EG31128"
/db_xref="GeneID:2847745"
ncRNA 16952..17006
/gene="sokC"
/locus_tag="b4413"
/gene_synonym="ECK0019; JWR0224; sof"
/ncRNA_class="antisense_RNA"
/product="sRNA antisense regulator blocking mokC, and
hence hokC, translation"
/note="IS186A interrupts hokC transcript downstream of
hokC gene in K-12"
/function="component of addiction module"
/db_xref="ASAP:ABE-0047238"
/db_xref="EcoGene:EG31128"
/db_xref="GeneID:2847745"
gene 17489..18655
/gene="nhaA"
/locus_tag="b0019"
/gene_synonym="ant; antA; ECK0020; JW0018"
/db_xref="EcoGene:EG10652"
/db_xref="GeneID:944758"
CDS 17489..18655
/gene="nhaA"
/locus_tag="b0019"
/gene_synonym="ant; antA; ECK0020; JW0018"
/function="transport; Transport of small molecules:
Cations"
/experiment="N-terminus verified by Edman degradation:
PMID 8381959"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/GO_process="GO:0009268 - response to pH"
/note="Na+/H antiporter, pH dependent"
/codon_start=1
/transl_table=11
/product="sodium-proton antiporter"
/protein_id="NP_414560.1"
/db_xref="GI:16128013"
/db_xref="ASAP:ABE-0000068"
/db_xref="UniProtKB/Swiss-Prot:P13738"
/db_xref="EcoGene:EG10652"
/db_xref="GeneID:944758"
/translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGARRTGVYILVGVVLWTA
VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
misc_feature 17489..18652
/gene="nhaA"
/locus_tag="b0019"
/gene_synonym="ant; antA; ECK0020; JW0018"
/note="pH-dependent sodium/proton antiporter; Reviewed;
Region: nhaA; PRK09561"
/db_xref="CDD:181955"
gene 18715..19620
/gene="nhaR"
/locus_tag="b0020"
/gene_synonym="antO; ECK0021; JW0019; yaaB"
/db_xref="EcoGene:EG11078"
/db_xref="GeneID:944757"
CDS 18715..19620
/gene="nhaR"
/locus_tag="b0020"
/gene_synonym="antO; ECK0021; JW0019; yaaB"
/function="regulator; Transport of small molecules:
Cations"
/GO_component="GO:0005737 - cytoplasm"
/GO_function="GO:0016563 - transcription activator
activity"
/GO_process="GO:0006350 - transcription"
/GO_process="GO:0009268 - response to pH"
/note="transcriptional activator of nhaA"
/codon_start=1
/transl_table=11
/product="DNA-binding transcriptional activator"
/protein_id="NP_414561.1"
/db_xref="GI:16128014"
/db_xref="ASAP:ABE-0000072"
/db_xref="UniProtKB/Swiss-Prot:P0A9G2"
/db_xref="EcoGene:EG11078"
/db_xref="GeneID:944757"
/translation="MSMSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRAL
EERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVA
DALSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQE
GLFSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNV
EILGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAE
RMIQHPAVQRICNTDYSALFSPAVR"
misc_feature 18721..19608
/gene="nhaR"
/locus_tag="b0020"
/gene_synonym="antO; ECK0021; JW0019; yaaB"
/note="transcriptional activator NhaR; Provisional;
Region: nhaR; PRK11062"
/db_xref="CDD:182938"
misc_feature 18739..18915
/gene="nhaR"
/locus_tag="b0020"
/gene_synonym="antO; ECK0021; JW0019; yaaB"
/note="Bacterial regulatory helix-turn-helix protein, lysR
family; Region: HTH_1; pfam00126"
/db_xref="CDD:201021"
misc_feature 19000..19602
/gene="nhaR"
/locus_tag="b0020"
/gene_synonym="antO; ECK0021; JW0019; yaaB"
/note="The C-terminal substrate binding domain of
LysR-type transcriptional activator of the nhaA gene,
encoding Na+/H+ antiporter, contains the type 2
periplasmic binding fold; Region: PBP2_NhaR; cd08429"
/db_xref="CDD:176120"
misc_feature order(19045..19050,19054..19059,19066..19068,19078..19080,
19084..19104,19375..19392,19408..19413,19417..19422)
/gene="nhaR"
/locus_tag="b0020"
/gene_synonym="antO; ECK0021; JW0019; yaaB"
/note="putative dimerization interface [polypeptide
binding]; other site"
/db_xref="CDD:176120"
mobile_element complement(19796..20563)
/mobile_element_type="insertion sequence:IS1A"
gene complement(19811..20314)
/gene="insB1"
/locus_tag="b0021"
/gene_synonym="ECK0022"
/db_xref="EcoGene:EG40002"
/db_xref="GeneID:944743"
CDS complement(19811..20314)
/gene="insB1"
/locus_tag="b0021"
/gene_synonym="ECK0022"
/function="IS, phage, Tn; Transposon-related functions;
extrachromosomal; transposon related"
/codon_start=1
/transl_table=11
/product="IS1 transposase B"
/protein_id="NP_414562.1"
/db_xref="GI:16128015"
/db_xref="ASAP:ABE-0000075"
/db_xref="UniProtKB/Swiss-Prot:P0CF25"
/db_xref="EcoGene:EG40002"
/db_xref="GeneID:944743"
/translation="MPGNSPHYGRWPQHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMD
EQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDG
WPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY
LNIKHYQ"
misc_feature complement(19814..20206)
/gene="insB1"
/locus_tag="b0021"
/gene_synonym="ECK0022"
/note="IS1 transposase; Region: DDE_Tnp_IS1; pfam03400"
/db_xref="CDD:112225"
gene complement(20233..20508)
/gene="insA"
/locus_tag="b0022"
/gene_synonym="ECK0023"
/note="insA1"
/db_xref="EcoGene:EG40001"
/db_xref="GeneID:948449"
CDS complement(20233..20508)
/gene="insA"
/locus_tag="b0022"
/gene_synonym="ECK0023"
/function="IS, phage, Tn; Transposon-related functions;
extrachromosomal; transposon related"
/codon_start=1
/transl_table=11
/product="IS1 repressor TnpA"
/protein_id="NP_414563.1"
/db_xref="GI:16128016"
/db_xref="ASAP:ABE-0000078"
/db_xref="UniProtKB/Swiss-Prot:P0CF07"
/db_xref="EcoGene:EG40001"
/db_xref="GeneID:948449"
/translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHLKNSGRSR"
misc_feature complement(20236..20508)
/gene="insA"
/locus_tag="b0022"
/gene_synonym="ECK0023"
/note="Transposase and inactivated derivatives [DNA
replication, recombination, and repair]; Region: COG3677"
/db_xref="CDD:226202"
misc_feature complement(20401..20508)
/gene="insA"
/locus_tag="b0022"
/gene_synonym="ECK0023"
/note="InsA N-terminal domain; Region: Zn_Tnp_IS1;
pfam03811"
/db_xref="CDD:190760"
misc_feature complement(20245..20394)
/gene="insA"
/locus_tag="b0022"
/gene_synonym="ECK0023"
/note="InsA C-terminal domain; Region: HTH_Tnp_IS1;
pfam12759"
/db_xref="CDD:193235"
gene complement(20815..21078)
/gene="rpsT"
/locus_tag="b0023"
/gene_synonym="ECK0024; JW0022; sup"
/db_xref="EcoGene:EG10919"
/db_xref="GeneID:944759"
CDS complement(20815..21078)
/gene="rpsT"
/locus_tag="b0023"
/gene_synonym="ECK0024; JW0022; sup"
/function="structural component; Ribosomal proteins -
synthesis, modification"
/GO_component="GO:0005737 - cytoplasm"
/GO_function="GO:0003735 - structural constituent of
ribosome"
/GO_process="GO:0006412 - translation"
/codon_start=1
/transl_table=11
/product="30S ribosomal subunit protein S20"
/protein_id="NP_414564.1"
/db_xref="GI:16128017"
/db_xref="ASAP:ABE-0000082"
/db_xref="UniProtKB/Swiss-Prot:P0A7U7"
/db_xref="EcoGene:EG10919"
/db_xref="GeneID:944759"
/translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
misc_feature complement(20818..21078)
/gene="rpsT"
/locus_tag="b0023"
/gene_synonym="ECK0024; JW0022; sup"
/note="30S ribosomal protein S20; Reviewed; Region: rpsT;
PRK00239"
/db_xref="CDD:178943"
mat_peptide complement(20818..21075)
/gene="rpsT"
/locus_tag="b0023"
/gene_synonym="ECK0024; JW0022; sup"
/product="30S ribosomal subunit protein S20"
/experiment="N-terminus verified by Edman degradation:
PMID 786731"
gene 21181..21399
/gene="yaaY"
/locus_tag="b0024"
/gene_synonym="ECK0025; JW5003"
/db_xref="EcoGene:EG14386"
/db_xref="GeneID:949128"
CDS 21181..21399
/gene="yaaY"
/locus_tag="b0024"
/gene_synonym="ECK0025; JW5003"
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="NP_414565.1"
/db_xref="GI:16128018"
/db_xref="ASAP:ABE-0000089"
/db_xref="UniProtKB/Swiss-Prot:P75620"
/db_xref="EcoGene:EG14386"
/db_xref="GeneID:949128"
/translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
SHFFLISVLNRRLTLTAVQGILGRFSLF"
misc_feature 21184..21396
/gene="yaaY"
/locus_tag="b0024"
/gene_synonym="ECK0025; JW5003"
/note="Protein of unknown function (DUF2575); Region:
DUF2575; pfam10837"
/db_xref="CDD:151286"
gene 21407..22348
/gene="ribF"
/locus_tag="b0025"
/gene_synonym="ECK0026; JW0023; yaaC"
/db_xref="EcoGene:EG11079"
/db_xref="GeneID:949129"
CDS 21407..22348
/gene="ribF"
/locus_tag="b0025"
/gene_synonym="ECK0026; JW0023; yaaC"
/EC_number="2.7.1.26"
/EC_number="2.7.7.2"
/function="putative regulator; Not classified"
/GO_component="GO:0005737 - cytoplasm"
/codon_start=1
/transl_table=11
/product="bifunctional riboflavin kinase/FAD synthetase"
/protein_id="NP_414566.1"
/db_xref="GI:16128019"
/db_xref="ASAP:ABE-0000091"
/db_xref="UniProtKB/Swiss-Prot:P0AG40"
/db_xref="EcoGene:EG11079"
/db_xref="GeneID:949129"
/translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
misc_feature 21410..22333
/gene="ribF"
/locus_tag="b0025"
/gene_synonym="ECK0026; JW0023; yaaC"
/note="bifunctional riboflavin kinase/FMN
adenylyltransferase; Reviewed; Region: PRK05627"
/db_xref="CDD:235536"
misc_feature 21458..22000
/gene="ribF"
/locus_tag="b0025"
/gene_synonym="ECK0026; JW0023; yaaC"
/note="FAD synthetase, N-terminal domain of the
bifunctional enzyme; Region: FAD_synthetase_N; cd02064"
/db_xref="CDD:185679"
misc_feature order(21473..21484,21491..21493,21500..21502,21782..21784,
21872..21874,21896..21904)
/gene="ribF"
/locus_tag="b0025"
/gene_synonym="ECK0026; JW0023; yaaC"
/note="active site"
/db_xref="CDD:185679"
misc_feature 21956..22327
/gene="ribF"
/locus_tag="b0025"
/gene_synonym="ECK0026; JW0023; yaaC"
/note="Riboflavin kinase; Region: Flavokinase; smart00904"
/db_xref="CDD:214901"
gene 22391..25207
/gene="ileS"
/locus_tag="b0026"
/gene_synonym="ECK0027; ilvS; JW0024"
/db_xref="EcoGene:EG10492"
/db_xref="GeneID:944761"
CDS 22391..25207
/gene="ileS"
/locus_tag="b0026"
/gene_synonym="ECK0027; ilvS; JW0024"
/EC_number="6.1.1.5"
/function="enzyme; Aminoacyl tRNA synthetases, tRNA
modification"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006418 - tRNA aminoacylation for protein
translation"
/note="isoleucine tRNA synthetase"
/codon_start=1
/transl_table=11
/product="isoleucyl-tRNA synthetase"
/protein_id="NP_414567.1"
/db_xref="GI:16128020"
/db_xref="ASAP:ABE-0000094"
/db_xref="UniProtKB/Swiss-Prot:P00956"
/db_xref="EcoGene:EG10492"
/db_xref="GeneID:944761"
/translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
AEHAEICGRCVSNVAGDGEKRKFA"
misc_feature 22391..25180
/gene="ileS"
/locus_tag="b0026"
/gene_synonym="ECK0027; ilvS; JW0024"
/note="isoleucyl-tRNA synthetase; Reviewed; Region: ileS;
PRK05743"
/db_xref="CDD:235588"
mat_peptide 22394..25204
/gene="ileS"
/locus_tag="b0026"
/gene_synonym="ECK0027; ilvS; JW0024"
/product="isoleucyl-tRNA synthetase"
/experiment="N-terminus verified by Edman degradation:
PMID 6390679,7929087"
misc_feature 22535..>22951
/gene="ileS"
/locus_tag="b0026"
/gene_synonym="ECK0027; ilvS; JW0024"
/note="catalytic core domain of isoleucyl-tRNA
synthetases; Region: IleRS_core; cd00818"
/db_xref="CDD:173909"
misc_feature 22583..22594
/gene="ileS"
/locus_tag="b0026"
/gene_synonym="ECK0027; ilvS; JW0024"
/note="HIGH motif; other site"
/db_xref="CDD:173909"
misc_feature <23624..24310
/gene="ileS"
/locus_tag="b0026"
/gene_synonym="ECK0027; ilvS; JW0024"
/note="catalytic core domain of isoleucyl-tRNA
synthetases; Region: IleRS_core; cd00818"
/db_xref="CDD:173909"
misc_feature order(23741..23743,23990..23992,23999..24001,24071..24085,
24089..24091,24095..24097,24164..24178,24188..24205,
24299..24301)
/gene="ileS"
/locus_tag="b0026"
/gene_synonym="ECK0027; ilvS; JW0024"
/note="active site"
/db_xref="CDD:173909"
misc_feature 24194..24208
/gene="ileS"
/locus_tag="b0026"
/gene_synonym="ECK0027; ilvS; JW0024"
/note="KMSKS motif; other site"
/db_xref="CDD:173909"
misc_feature 24308..24850
/gene="ileS"
/locus_tag="b0026"
/gene_synonym="ECK0027; ilvS; JW0024"
/note="Anticodon-binding domain of bacterial and
eukaryotic mitochondrial isoleucyl tRNA synthetases;
Region: Anticodon_Ia_Ile_BEm; cd07960"
/db_xref="CDD:153414"
misc_feature order(24317..24319,24329..24331,24338..24340,24350..24352,
24359..24361,24368..24373,24515..24517,24524..24529,
24536..24541,24548..24550,24563..24565,24575..24577,
24584..24586,24839..24841,24848..24850)
/gene="ileS"
/locus_tag="b0026"
/gene_synonym="ECK0027; ilvS; JW0024"
/note="tRNA binding surface [nucleotide binding]; other
site"
/db_xref="CDD:153414"
misc_feature order(24359..24361,24368..24370)
/gene="ileS"
/locus_tag="b0026"
/gene_synonym="ECK0027; ilvS; JW0024"
/note="anticodon binding site; other site"
/db_xref="CDD:153414"
misc_feature 25082..25171
/gene="ileS"
/locus_tag="b0026"
/gene_synonym="ECK0027; ilvS; JW0024"
/note="Zinc finger found in FPG and IleRS; Region:
zf-FPG_IleRS; pfam06827"
/db_xref="CDD:203527"
gene 25207..25701
/gene="lspA"
/locus_tag="b0027"
/gene_synonym="ECK0028; JW0025"
/db_xref="EcoGene:EG10548"
/db_xref="GeneID:944800"
CDS 25207..25701
/gene="lspA"
/locus_tag="b0027"
/gene_synonym="ECK0028; JW0025"
/EC_number="3.4.23.36"
/function="enzyme; Protein, peptide secretion"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/note="prolipoprotein signal peptidase (SPase II)"
/codon_start=1
/transl_table=11
/product="prolipoprotein signal peptidase (signal
peptidase II)"
/protein_id="NP_414568.1"
/db_xref="GI:16128021"
/db_xref="ASAP:ABE-0000096"
/db_xref="UniProtKB/Swiss-Prot:P00804"
/db_xref="EcoGene:EG10548"
/db_xref="GeneID:944800"
/translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
AKKQ"
misc_feature 25267..25686
/gene="lspA"
/locus_tag="b0027"
/gene_synonym="ECK0028; JW0025"
/note="lipoprotein signal peptidase; Reviewed; Region:
lspA; PRK00376"
/db_xref="CDD:234739"
repeat_region 25710..25795
/note="REP2 (repetitive extragenic palindromic) element;
contains 2 REP sequences"
gene 25826..26275
/gene="fkpB"
/locus_tag="b0028"
/gene_synonym="ECK0029; JW0026; slpA; yaaD"
/db_xref="EcoGene:EG11080"
/db_xref="GeneID:944807"
CDS 25826..26275
/gene="fkpB"
/locus_tag="b0028"
/gene_synonym="ECK0029; JW0026; slpA; yaaD"
/EC_number="5.2.1.8"
/function="putative enzyme; Proteins - translation and
modification"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006457 - protein folding"
/codon_start=1
/transl_table=11
/product="FKBP-type peptidyl-prolyl cis-trans isomerase
(rotamase)"
/protein_id="NP_414569.1"
/db_xref="GI:16128022"
/db_xref="ASAP:ABE-0000101"
/db_xref="UniProtKB/Swiss-Prot:P0AEM0"
/db_xref="EcoGene:EG11080"
/db_xref="GeneID:944807"
/translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
misc_feature 25826..26272
/gene="fkpB"
/locus_tag="b0028"
/gene_synonym="ECK0029; JW0026; slpA; yaaD"
/note="FKBP-type peptidyl-prolyl cis-trans isomerase;
Provisional; Region: PRK15095"
/db_xref="CDD:237908"
misc_feature 25832..>26041
/gene="fkpB"
/locus_tag="b0028"
/gene_synonym="ECK0029; JW0026; slpA; yaaD"
/note="FKBP-type peptidyl-prolyl cis-trans isomerase;
Region: FKBP_C; pfam00254"
/db_xref="CDD:215821"
gene 26277..27227
/gene="ispH"
/locus_tag="b0029"
/gene_synonym="ECK0030; JW0027; lytB; yaaE"
/db_xref="EcoGene:EG11081"
/db_xref="GeneID:944777"
CDS 26277..27227
/gene="ispH"
/locus_tag="b0029"
/gene_synonym="ECK0030; JW0027; lytB; yaaE"
/EC_number="1.17.1.2"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0042493 - response to drug"
/codon_start=1
/transl_table=11
/product="4-hydroxy-3-methylbut-2-enyl diphosphate
reductase, 4Fe-4S protein"
/protein_id="NP_414570.1"
/db_xref="GI:16128023"
/db_xref="ASAP:ABE-0000103"
/db_xref="UniProtKB/Swiss-Prot:P62623"
/db_xref="EcoGene:EG11081"
/db_xref="GeneID:944777"
/translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
misc_feature 26277..27179
/gene="ispH"
/locus_tag="b0029"
/gene_synonym="ECK0030; JW0027; lytB; yaaE"
/note="4-hydroxy-3-methylbut-2-enyl diphosphate reductase;
Reviewed; Region: ispH; PRK01045"
/db_xref="CDD:234893"
misc_feature 26277..27161
/gene="ispH"
/locus_tag="b0029"
/gene_synonym="ECK0030; JW0027; lytB; yaaE"
/note="4-Hydroxy-3-methylbut-2-enyl diphosphate reductase
IspH [Lipid metabolism]; Region: lytB; COG0761"
/db_xref="CDD:223832"
gene 27293..28207
/gene="rihC"
/locus_tag="b0030"
/gene_synonym="ECK0031; JW0028; yaaF"
/db_xref="EcoGene:EG11082"
/db_xref="GeneID:944796"
CDS 27293..28207
/gene="rihC"
/locus_tag="b0030"
/gene_synonym="ECK0031; JW0028; yaaF"
/EC_number="3.2.2.-"
/GO_process="GO:0009226 - nucleotide-sugar biosynthetic
process"
/codon_start=1
/transl_table=11
/product="ribonucleoside hydrolase 3"
/protein_id="NP_414571.1"
/db_xref="GI:16128024"
/db_xref="ASAP:ABE-0000109"
/db_xref="UniProtKB/Swiss-Prot:P22564"
/db_xref="EcoGene:EG11082"
/db_xref="GeneID:944796"
/translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
KPANVQVALDLDVKGFQQWVAEVLALAS"
misc_feature 27302..28192
/gene="rihC"
/locus_tag="b0030"
/gene_synonym="ECK0031; JW0028; yaaF"
/note="nuc_hydro_IU_UC_XIUA: inosine-uridine preferring,
xanthosine-inosine-uridine-adenosine-preferring and,
uridine-cytidine preferring nucleoside hydrolases.
Nucleoside hydrolases cleave the N-glycosidic bond in
nucleosides generating ribose and the...; Region:
nuc_hydro_IU_UC_XIUA; cd02651"
/db_xref="CDD:239117"
misc_feature order(27320..27322,27332..27337,27407..27409,27530..27532,
27659..27661,27761..27763,27779..27781,27785..27787,
27992..27994)
/gene="rihC"
/locus_tag="b0030"
/gene_synonym="ECK0031; JW0028; yaaF"
/note="active site"
/db_xref="CDD:239117"
misc_feature order(27500..27502,27692..27694,27815..27817,28061..28063,
28067..28072,28079..28081)
/gene="rihC"
/locus_tag="b0030"
/gene_synonym="ECK0031; JW0028; yaaF"
/note="tetramer interface [polypeptide binding]; other
site"
/db_xref="CDD:239117"
gene 28374..29195
/gene="dapB"
/locus_tag="b0031"
/gene_synonym="ECK0032; JW0029"
/db_xref="EcoGene:EG10206"
/db_xref="GeneID:944762"
CDS 28374..29195
/gene="dapB"
/locus_tag="b0031"
/gene_synonym="ECK0032; JW0029"
/EC_number="1.3.1.26"
/function="enzyme; Amino acid biosynthesis: Lysine"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0009089 - lysine biosynthetic process via
diaminopimelate"
/codon_start=1
/transl_table=11
/product="dihydrodipicolinate reductase"
/protein_id="NP_414572.1"
/db_xref="GI:16128025"
/db_xref="ASAP:ABE-0000112"
/db_xref="UniProtKB/Swiss-Prot:P04036"
/db_xref="EcoGene:EG10206"
/db_xref="GeneID:944762"
/translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
misc_feature 28389..29183
/gene="dapB"
/locus_tag="b0031"
/gene_synonym="ECK0032; JW0029"
/note="Dihydrodipicolinate reductase [Amino acid transport
and metabolism]; Region: DapB; COG0289"
/db_xref="CDD:223366"
misc_feature 28389..28709
/gene="dapB"
/locus_tag="b0031"
/gene_synonym="ECK0032; JW0029"
/note="Dihydrodipicolinate reductase, N-terminus; Region:
DapB_N; pfam01113"
/db_xref="CDD:216304"
misc_feature 28767..29177
/gene="dapB"
/locus_tag="b0031"
/gene_synonym="ECK0032; JW0029"
/note="Dihydrodipicolinate reductase, C-terminus; Region:
DapB_C; pfam05173"
/db_xref="CDD:218479"
gene 29651..30799
/gene="carA"
/locus_tag="b0032"
/gene_synonym="arg; cap; ECK0033; JW0030; pyrA"
/db_xref="EcoGene:EG10134"
/db_xref="GeneID:949025"
CDS 29651..30799
/gene="carA"
/locus_tag="b0032"
/gene_synonym="arg; cap; ECK0033; JW0030; pyrA"
/EC_number="6.3.5.5"
/function="enzyme; Pyrimidine ribonucleotide biosynthesis"
/function="1.5.1.3 metabolism; building block
biosynthesis; amino acids; arginine"
/function="1.5.2.2 metabolism; building block
biosynthesis; nucleotide; pyrimidine biosynthesis"
/experiment="N-terminus verified by Edman degradation:
PMID 3894020,9298646,9740056"
/GO_process="GO:0006526 - arginine biosynthetic process"
/GO_process="GO:0006221 - pyrimidine nucleotide
biosynthetic process"
/codon_start=1
/transl_table=11
/product="carbamoyl phosphate synthetase small subunit,
glutamine amidotransferase"
/protein_id="NP_414573.1"
/db_xref="GI:16128026"
/db_xref="ASAP:ABE-0000118"
/db_xref="UniProtKB/Swiss-Prot:P0A6F1"
/db_xref="EcoGene:EG10134"
/db_xref="GeneID:949025"
/translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
misc_feature 29651..30772
/gene="carA"
/locus_tag="b0032"
/gene_synonym="arg; cap; ECK0033; JW0030; pyrA"
/note="carbamoyl phosphate synthase small subunit;
Reviewed; Region: PRK12564"
/db_xref="CDD:237139"
misc_feature 29657..30046
/gene="carA"
/locus_tag="b0032"
/gene_synonym="arg; cap; ECK0033; JW0030; pyrA"
/note="Carbamoyl-phosphate synthase small chain, CPSase
domain; Region: CPSase_sm_chain; smart01097"
/db_xref="CDD:198165"
misc_feature 30230..30763
/gene="carA"
/locus_tag="b0032"
/gene_synonym="arg; cap; ECK0033; JW0030; pyrA"
/note="Small chain of the glutamine-dependent form of
carbamoyl phosphate synthase, CPSase II; Region:
GATase1_CPSase; cd01744"
/db_xref="CDD:153215"
misc_feature order(30455..30457,30707..30709,30713..30715)
/gene="carA"
/locus_tag="b0032"
/gene_synonym="arg; cap; ECK0033; JW0030; pyrA"
/note="catalytic site [active]"
/db_xref="CDD:153215"
misc_feature order(30530..30532,30536..30538,30557..30559,30563..30565,
30644..30646,30722..30727,30731..30736)
/gene="carA"
/locus_tag="b0032"
/gene_synonym="arg; cap; ECK0033; JW0030; pyrA"
/note="subunit interface [polypeptide binding]; other
site"
/db_xref="CDD:153215"
gene 30817..34038
/gene="carB"
/locus_tag="b0033"
/gene_synonym="cap; ECK0034; JW0031; pyrA"
/db_xref="EcoGene:EG10135"
/db_xref="GeneID:944775"
CDS 30817..34038
/gene="carB"
/locus_tag="b0033"
/gene_synonym="cap; ECK0034; JW0031; pyrA"
/EC_number="6.3.5.5"
/function="enzyme; Pyrimidine ribonucleotide biosynthesis"
/function="1.5.1.3 metabolism; building block
biosynthesis; amino acids; arginine"
/function="1.5.2.2 metabolism; building block
biosynthesis; nucleotide; pyrimidine biosynthesis"
/GO_process="GO:0006526 - arginine biosynthetic process"
/GO_process="GO:0006221 - pyrimidine nucleotide
biosynthetic process"
/codon_start=1
/transl_table=11
/product="carbamoyl-phosphate synthase large subunit"
/protein_id="NP_414574.1"
/db_xref="GI:16128027"
/db_xref="ASAP:ABE-0000121"
/db_xref="UniProtKB/Swiss-Prot:P00968"
/db_xref="EcoGene:EG10135"
/db_xref="GeneID:944775"
/translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGADRIWYIADAFRAGLSVD
GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGV
REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
misc_feature 30817..34023
/gene="carB"
/locus_tag="b0033"
/gene_synonym="cap; ECK0034; JW0031; pyrA"
/note="carbamoyl phosphate synthase large subunit;
Reviewed; Region: carB; PRK05294"
/db_xref="CDD:235393"
mat_peptide 30820..34035
/gene="carB"
/locus_tag="b0033"
/gene_synonym="cap; ECK0034; JW0031; pyrA"
/product="carbamoyl-phosphate synthase large subunit"
/experiment="N-terminus verified by Edman degradation:
PMID 6308632"
misc_feature 30835..31182
/gene="carB"
/locus_tag="b0033"
/gene_synonym="cap; ECK0034; JW0031; pyrA"
/note="Carbamoyl-phosphate synthase L chain, N-terminal
domain; Region: CPSase_L_chain; pfam00289"
/db_xref="CDD:201133"
misc_feature 31198..31821
/gene="carB"
/locus_tag="b0033"
/gene_synonym="cap; ECK0034; JW0031; pyrA"
/note="Carbamoyl-phosphate synthase L chain, ATP binding
domain; Region: CPSase_L_D2; pfam02786"
/db_xref="CDD:190425"
misc_feature 32086..32457
/gene="carB"
/locus_tag="b0033"
/gene_synonym="cap; ECK0034; JW0031; pyrA"
/note="Carbamoyl-phosphate synthetase large chain,
oligomerisation domain; Region: CPSase_L_D3; pfam02787"
/db_xref="CDD:217231"
misc_feature 32494..32820
/gene="carB"
/locus_tag="b0033"
/gene_synonym="cap; ECK0034; JW0031; pyrA"
/note="Carbamoyl-phosphate synthase L chain, N-terminal
domain; Region: CPSase_L_chain; pfam00289"
/db_xref="CDD:201133"
misc_feature 32836..33444
/gene="carB"
/locus_tag="b0033"
/gene_synonym="cap; ECK0034; JW0031; pyrA"
/note="ATP-grasp domain; Region: ATP-grasp_4; cl17255"
/db_xref="CDD:247809"
misc_feature 33643..33972
/gene="carB"
/locus_tag="b0033"
/gene_synonym="cap; ECK0034; JW0031; pyrA"
/note="Methylglyoxal synthase-like domain from type II
glutamine-dependent carbamoyl phosphate synthetase (CSP).
CSP, a CarA and CarB heterodimer, catalyzes the production
of carbamoyl phosphate which is subsequently employed in
the metabolic pathways...; Region: MGS_CPS_II; cd01424"
/db_xref="CDD:238712"
misc_feature order(33658..33660,33676..33678,33736..33738,33742..33747,
33793..33795,33859..33867)
/gene="carB"
/locus_tag="b0033"
/gene_synonym="cap; ECK0034; JW0031; pyrA"
/note="IMP binding site; other site"
/db_xref="CDD:238712"
misc_feature order(33739..33741,33751..33753,33775..33777,33781..33786,
33790..33792,33826..33828)
/gene="carB"
/locus_tag="b0033"
/gene_synonym="cap; ECK0034; JW0031; pyrA"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:238712"
misc_feature order(33934..33936,33949..33951,33961..33963)
/gene="carB"
/locus_tag="b0033"
/gene_synonym="cap; ECK0034; JW0031; pyrA"
/note="interdomain contacts; other site"
/db_xref="CDD:238712"
misc_feature 33934..33942
/gene="carB"
/locus_tag="b0033"
/gene_synonym="cap; ECK0034; JW0031; pyrA"
/note="partial ornithine binding site; other site"
/db_xref="CDD:238712"
gene 34300..34695
/gene="caiF"
/locus_tag="b0034"
/gene_synonym="ECK0035; JW0033"
/db_xref="EcoGene:EG13451"
/db_xref="GeneID:944795"
CDS 34300..34695
/gene="caiF"
/locus_tag="b0034"
/gene_synonym="ECK0035; JW0033"
/function="regulator; Central intermediary metabolism:
Pool, multipurpose conversions"
/GO_component="GO:0005737 - cytoplasm"
/GO_function="GO:0016563 - transcription activator
activity"
/GO_process="GO:0009310 - amine catabolic process"
/GO_process="GO:0006350 - transcription"
/note="transcriptional regulator of cai operon"
/codon_start=1
/transl_table=11
/product="DNA-binding transcriptional activator"
/protein_id="NP_414576.4"
/db_xref="GI:90111079"
/db_xref="ASAP:ABE-0000125"
/db_xref="UniProtKB/Swiss-Prot:P0AE58"
/db_xref="EcoGene:EG13451"
/db_xref="GeneID:944795"
/translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
KTPRIPAVPLTELNREQKWQMMLSKSMRR"
misc_feature 34354..34692
/gene="caiF"
/locus_tag="b0034"
/gene_synonym="ECK0035; JW0033"
/note="DNA-binding transcriptional activator CaiF;
Provisional; Region: PRK11476"
/db_xref="CDD:183154"
repeat_region 34727..34762
/note="REP3 (repetitive extragenic palindromic) element;
contains 1 REP sequences"
gene complement(34781..35371)
/gene="caiE"
/locus_tag="b0035"
/gene_synonym="ECK0036; JW5004"
/db_xref="EcoGene:EG12608"
/db_xref="GeneID:948999"
CDS complement(34781..35371)
/gene="caiE"
/locus_tag="b0035"
/gene_synonym="ECK0036; JW5004"
/function="putative enzyme; Central intermediary
metabolism: Pool, multipurpose conversions"
/GO_process="GO:0009310 - amine catabolic process"
/GO_process="GO:0042413 - carnitine catabolic process"
/note="possible synthesis of cofactor for carnitine
racemase and dehydratase"
/codon_start=1
/transl_table=11
/product="stimulator of CaiD and CaiB enzyme activities"
/protein_id="NP_414577.2"
/db_xref="GI:90111080"
/db_xref="ASAP:ABE-0000128"
/db_xref="UniProtKB/Swiss-Prot:P39206"
/db_xref="EcoGene:EG12608"
/db_xref="GeneID:948999"
/translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCLIGRDALVGMNSV
IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRNVSDDELHWKRLNTKEYQ
DLVGRCHVSLHETQPLRQMEENRPRLQGTTDVTPKR"
misc_feature complement(34784..35371)
/gene="caiE"
/locus_tag="b0035"
/gene_synonym="ECK0036; JW5004"
/note="carnitine operon protein CaiE; Provisional; Region:
PRK13627"
/db_xref="CDD:184189"
misc_feature complement(34877..35341)
/gene="caiE"
/locus_tag="b0035"
/gene_synonym="ECK0036; JW5004"
/note="paaY-like: This group is composed by
uncharacterized proteins with similarity to the protein
product of the E. coli paaY gene, which is part of the paa
gene cluster responsible for phenylacetic acid
degradation. Proteins in this group are expected to...;
Region: LbH_paaY_like; cd04745"
/db_xref="CDD:100058"
misc_feature complement(order(35075..35077,35111..35113,35117..35128,
35177..35179,35189..35194,35234..35236,35240..35242,
35246..35251,35258..35260,35294..35296,35303..35308,
35312..35314,35318..35320))
/gene="caiE"
/locus_tag="b0035"
/gene_synonym="ECK0036; JW5004"
/note="putative trimer interface [polypeptide binding];
other site"
/db_xref="CDD:100058"
misc_feature complement(order(35111..35113,35126..35128,35177..35179))
/gene="caiE"
/locus_tag="b0035"
/gene_synonym="ECK0036; JW5004"
/note="putative metal binding site [ion binding]; other
site"
/db_xref="CDD:100058"
gene complement(35377..36162)
/gene="caiD"
/locus_tag="b0036"
/gene_synonym="ECK0037; JW0035; yaaL"
/db_xref="EcoGene:EG11557"
/db_xref="GeneID:948995"
CDS complement(35377..36162)
/gene="caiD"
/locus_tag="b0036"
/gene_synonym="ECK0037; JW0035; yaaL"
/EC_number="4.2.1.-"
/function="enzyme; Degradation of small molecules: Amines"
/GO_process="GO:0009310 - amine catabolic process"
/GO_process="GO:0042413 - carnitine catabolic process"
/note="carnitine racemase"
/codon_start=1
/transl_table=11
/product="carnitinyl-CoA dehydratase"
/protein_id="NP_414578.2"
/db_xref="GI:221800779"
/db_xref="ASAP:ABE-0000130"
/db_xref="UniProtKB/Swiss-Prot:P31551"
/db_xref="EcoGene:EG11557"
/db_xref="GeneID:948995"
/translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
misc_feature complement(35380..36162)
/gene="caiD"
/locus_tag="b0036"
/gene_synonym="ECK0037; JW0035; yaaL"
/note="carnitinyl-CoA dehydratase; Provisional; Region:
PRK03580"
/db_xref="CDD:179599"
misc_feature complement(35575..36150)
/gene="caiD"
/locus_tag="b0036"
/gene_synonym="ECK0037; JW0035; yaaL"
/note="Crotonase/Enoyl-Coenzyme A (CoA) hydratase
superfamily. This superfamily contains a diverse set of
enzymes including enoyl-CoA hydratase, napthoate synthase,
methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA
dehydratase, and dienoyl-CoA isomerase; Region:
crotonase-like; cd06558"
/db_xref="CDD:119339"
misc_feature complement(order(35761..35763,35770..35775,35839..35847,
35851..35853,35965..35979,35989..35991,36088..36090,
36094..36096))
/gene="caiD"
/locus_tag="b0036"
/gene_synonym="ECK0037; JW0035; yaaL"
/note="substrate binding site [chemical binding]; other
site"
/db_xref="CDD:119339"
misc_feature complement(order(35839..35841,35971..35973))
/gene="caiD"
/locus_tag="b0036"
/gene_synonym="ECK0037; JW0035; yaaL"
/note="oxyanion hole (OAH) forming residues; other site"
/db_xref="CDD:119339"
misc_feature complement(order(35575..35580,35587..35589,35632..35634,
35641..35643,35674..35676,35683..35688,35692..35697,
35701..35706,35719..35724,35728..35736,35740..35742,
35758..35769,35803..35814,35875..35877,35899..35901))
/gene="caiD"
/locus_tag="b0036"
/gene_synonym="ECK0037; JW0035; yaaL"
/note="trimer interface [polypeptide binding]; other site"
/db_xref="CDD:119339"
gene complement(36271..37824)
/gene="caiC"
/locus_tag="b0037"
/gene_synonym="ECK0038; JW0036; yaaM"
/db_xref="EcoGene:EG11558"
/db_xref="GeneID:944886"
CDS complement(36271..37824)
/gene="caiC"
/locus_tag="b0037"
/gene_synonym="ECK0038; JW0036; yaaM"
/EC_number="6.3.2.-"
/function="putative enzyme; Central intermediary
metabolism: Pool, multipurpose conversions"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/GO_process="GO:0009310 - amine catabolic process"
/note="probable crotonobetaine/carnitine-CoA ligase"
/codon_start=1
/transl_table=11
/product="putative crotonobetaine/carnitine-CoA ligase"
/protein_id="NP_414579.4"
/db_xref="GI:221800780"
/db_xref="ASAP:ABE-0000133"
/db_xref="UniProtKB/Swiss-Prot:P31552"
/db_xref="EcoGene:EG11558"
/db_xref="GeneID:944886"
/translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLCEESA
WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
MVQPPSANDQQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
DKRRWPSIGRVGFCYEAEIRDDHNRPLPAGEIGEICIKGIPGKTIFKEYFLNPQATAK
VLEADGWLHTGDTGYRDEEDFFYFVDRRCNMIKRGGENVSCVELENIIAAHPKIQDIV
VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
GKIIRKNLK"
misc_feature complement(36274..37824)
/gene="caiC"
/locus_tag="b0037"
/gene_synonym="ECK0038; JW0036; yaaM"
/note="putative crotonobetaine/carnitine-CoA ligase;
Validated; Region: caiC; PRK08008"
/db_xref="CDD:181195"
misc_feature complement(36274..37719)
/gene="caiC"
/locus_tag="b0037"
/gene_synonym="ECK0038; JW0036; yaaM"
/note="Uncharacterized subfamily of fatty acid CoA ligase
(FACL); Region: FACL_DitJ_like; cd05934"
/db_xref="CDD:213300"
misc_feature complement(order(37258..37263,37267..37284,37291..37293))
/gene="caiC"
/locus_tag="b0037"
/gene_synonym="ECK0038; JW0036; yaaM"
/note="acyl-activating enzyme (AAE) consensus motif; other
site"
/db_xref="CDD:213300"
misc_feature complement(order(36295..36297,36535..36537,36568..36570,
36577..36579,36613..36615,36859..36876,36934..36939,
37282..37284))
/gene="caiC"
/locus_tag="b0037"
/gene_synonym="ECK0038; JW0036; yaaM"
/note="putative AMP binding site [chemical binding]; other
site"
/db_xref="CDD:213300"
misc_feature complement(order(36352..36354,36535..36546,36568..36570,
36577..36579,36613..36615,36859..36876,36934..36939,
36994..36996,37003..37008,37012..37014,37159..37164,
37282..37284))
/gene="caiC"
/locus_tag="b0037"
/gene_synonym="ECK0038; JW0036; yaaM"
/note="putative active site [active]"
/db_xref="CDD:213300"
misc_feature complement(order(36352..36354,36370..36372,36538..36546,
36937..36939,36994..36996,37003..37008,37162..37164))
/gene="caiC"
/locus_tag="b0037"
/gene_synonym="ECK0038; JW0036; yaaM"
/note="putative CoA binding site [chemical binding]; other
site"
/db_xref="CDD:213300"
gene complement(37898..39115)
/gene="caiB"
/locus_tag="b0038"
/gene_synonym="ECK0039; JW0037; yaaN"
/db_xref="EcoGene:EG11559"
/db_xref="GeneID:948997"
CDS complement(37898..39115)
/gene="caiB"
/locus_tag="b0038"
/gene_synonym="ECK0039; JW0037; yaaN"
/EC_number="2.8.3.-"
/function="enzyme; Central intermediary metabolism: Pool,
multipurpose conversions"
/experiment="N-terminus verified by Edman degradation:
PMID 8188598,10209289"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0042413 - carnitine catabolic process"
/GO_process="GO:0009310 - amine catabolic process"
/note="l-carnitine dehydratase"
/codon_start=1
/transl_table=11
/product="crotonobetainyl CoA:carnitine CoA transferase"
/protein_id="NP_414580.1"
/db_xref="GI:16128032"
/db_xref="ASAP:ABE-0000136"
/db_xref="UniProtKB/Swiss-Prot:P31572"
/db_xref="EcoGene:EG11559"
/db_xref="GeneID:948997"
/translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
GTQLIHRIECPYGPLVEEKLDAWLATHTIAEVKERFAELNIACAKVLTVPELESNPQY
VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
QELVSKGLAKVED"
misc_feature complement(37901..39115)
/gene="caiB"
/locus_tag="b0038"
/gene_synonym="ECK0039; JW0037; yaaN"
/note="crotonobetainyl-CoA:carnitine CoA-transferase;
Provisional; Region: PRK03525"
/db_xref="CDD:179589"
misc_feature complement(38372..38911)
/gene="caiB"
/locus_tag="b0038"
/gene_synonym="ECK0039; JW0037; yaaN"
/note="CoA-transferase family III; Region: CoA_transf_3;
pfam02515"
/db_xref="CDD:217078"
repeat_region 39151..39232
/note="REP4 (repetitive extragenic palindromic) element;
contains 2 REP sequences"
gene complement(39244..40386)
/gene="caiA"
/locus_tag="b0039"
/gene_synonym="ECK0040; JW0038; yaaO"
/db_xref="EcoGene:EG11560"
/db_xref="GeneID:949064"
CDS complement(39244..40386)
/gene="caiA"
/locus_tag="b0039"
/gene_synonym="ECK0040; JW0038; yaaO"
/EC_number="1.3.99.-"
/function="putative regulator; Central intermediary
metabolism: Pool, multipurpose conversions"
/experiment="N-terminus verified by Edman degradation:
PMID 10209289"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006097 - glyoxylate cycle"
/GO_process="GO:0009310 - amine catabolic process"
/GO_process="GO:0042413 - carnitine catabolic process"
/note="probable carnitine operon oxidoreductase"
/codon_start=1
/transl_table=11
/product="crotonobetaine reductase subunit II,
FAD-binding"
/protein_id="NP_414581.1"
/db_xref="GI:16128033"
/db_xref="ASAP:ABE-0000139"
/db_xref="UniProtKB/Swiss-Prot:P60584"
/db_xref="EcoGene:EG11560"
/db_xref="GeneID:949064"
/translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
misc_feature complement(39247..40386)
/gene="caiA"
/locus_tag="b0039"
/gene_synonym="ECK0040; JW0038; yaaO"
/note="crotonobetainyl-CoA dehydrogenase; Validated;
Region: PRK03354"
/db_xref="CDD:179566"
misc_feature complement(39268..40368)
/gene="caiA"
/locus_tag="b0039"
/gene_synonym="ECK0040; JW0038; yaaO"
/note="Acyl-CoA dehydrogenase; Region: ACAD; cd00567"
/db_xref="CDD:173838"
misc_feature complement(order(39292..39294,39298..39300,39304..39312,
39916..39918,39922..39924,40015..40017,40021..40023,
40237..40239))
/gene="caiA"
/locus_tag="b0039"
/gene_synonym="ECK0040; JW0038; yaaO"
/note="active site"
/db_xref="CDD:173838"
gene complement(40417..41931)
/gene="caiT"
/locus_tag="b0040"
/gene_synonym="ECK0041; JW0039; yaaP"
/db_xref="EcoGene:EG11561"
/db_xref="GeneID:944765"
CDS complement(40417..41931)
/gene="caiT"
/locus_tag="b0040"
/gene_synonym="ECK0041; JW0039; yaaP"
/function="putative transport; Central intermediary
metabolism: Pool, multipurpose conversions"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/GO_process="GO:0016052 - carbohydrate catabolic process"
/note="probable carnitine transporter"
/codon_start=1
/transl_table=11
/product="putative transporter"
/protein_id="NP_414582.1"
/db_xref="GI:16128034"
/db_xref="ASAP:ABE-0000141"
/db_xref="UniProtKB/Swiss-Prot:P31553"
/db_xref="EcoGene:EG11561"
/db_xref="GeneID:944765"
/translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
misc_feature complement(40420..41931)
/gene="caiT"
/locus_tag="b0040"
/gene_synonym="ECK0041; JW0039; yaaP"
/note="L-carnitine/gamma-butyrobetaine antiporter;
Provisional; Region: PRK03356"
/db_xref="CDD:179568"
gene 42403..43173
/gene="fixA"
/locus_tag="b0041"
/gene_synonym="ECK0042; JW0040; yaaQ"
/db_xref="EcoGene:EG11562"
/db_xref="GeneID:947316"
CDS 42403..43173
/gene="fixA"
/locus_tag="b0041"
/gene_synonym="ECK0042; JW0040; yaaQ"
/function="putative enzyme; Energy metabolism, carbon:
Electron transport"
/GO_process="GO:0016052 - carbohydrate catabolic process"
/note="probable flavoprotein subunit, carnitine
metabolism"
/codon_start=1
/transl_table=11
/product="putative electron transfer flavoprotein subunit,
required for anaerobic carnitine reduction"
/protein_id="NP_414583.2"
/db_xref="GI:90111081"
/db_xref="ASAP:ABE-0000145"
/db_xref="UniProtKB/Swiss-Prot:P60566"
/db_xref="EcoGene:EG11562"
/db_xref="GeneID:947316"
/translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
misc_feature 42406..43023
/gene="fixA"
/locus_tag="b0041"
/gene_synonym="ECK0042; JW0040; yaaQ"
/note="The electron transfer flavoprotein (ETF) serves as
a specific electron acceptor for various mitochondrial
dehydrogenases. ETF transfers electrons to the main
respiratory chain via ETF-ubiquinone oxidoreductase. ETF
is an heterodimer that consists of an...; Region:
ETF_beta; cd01714"
/db_xref="CDD:238847"
misc_feature order(42418..42423,42505..42507,42514..42516,42586..42588,
42754..42759,42763..42768,42781..42792)
/gene="fixA"
/locus_tag="b0041"
/gene_synonym="ECK0042; JW0040; yaaQ"
/note="Ligand binding site [chemical binding]; other site"
/db_xref="CDD:238847"
misc_feature 42490..42963
/gene="fixA"
/locus_tag="b0041"
/gene_synonym="ECK0042; JW0040; yaaQ"
/note="Electron transfer flavoprotein domain; Region: ETF;
pfam01012"
/db_xref="CDD:216243"
gene 43188..44129
/gene="fixB"
/locus_tag="b0042"
/gene_synonym="ECK0043; JW0041; yaaR"
/db_xref="EcoGene:EG11563"
/db_xref="GeneID:948939"
CDS 43188..44129
/gene="fixB"
/locus_tag="b0042"
/gene_synonym="ECK0043; JW0041; yaaR"
/function="putative enzyme; Energy metabolism, carbon:
Electron transport"
/GO_process="GO:0016052 - carbohydrate catabolic process"
/note="probable flavoprotein subunit, carnitine
metabolism"
/codon_start=1
/transl_table=11
/product="putative electron transfer flavoprotein,
NAD/FAD-binding domain and ETFP adenine nucleotide-binding
domain-like protein"
/protein_id="NP_414584.1"
/db_xref="GI:16128036"
/db_xref="ASAP:ABE-0000147"
/db_xref="UniProtKB/Swiss-Prot:P31574"
/db_xref="EcoGene:EG11563"
/db_xref="GeneID:948939"
/translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
misc_feature 43188..44126
/gene="fixB"
/locus_tag="b0042"
/gene_synonym="ECK0043; JW0041; yaaR"
/note="putative electron transfer flavoprotein FixB;
Provisional; Region: fixB; PRK03363"
/db_xref="CDD:235120"
misc_feature <43311..43682
/gene="fixB"
/locus_tag="b0042"
/gene_synonym="ECK0043; JW0041; yaaR"
/note="The electron transfer flavoprotein (ETF) serves as
a specific electron acceptor for various mitochondrial
dehydrogenases. ETF transfers electrons to the main
respiratory chain via ETF-ubiquinone oxidoreductase. ETF
is an heterodimer that consists of an...; Region: ETF;
cd01985"
/db_xref="CDD:238943"
misc_feature order(43443..43448,43452..43457,43461..43472)
/gene="fixB"
/locus_tag="b0042"
/gene_synonym="ECK0043; JW0041; yaaR"
/note="Ligand binding site [chemical binding]; other site"
/db_xref="CDD:238943"
misc_feature 43752..44012
/gene="fixB"
/locus_tag="b0042"
/gene_synonym="ECK0043; JW0041; yaaR"
/note="Electron transfer flavoprotein FAD-binding domain;
Region: ETF_alpha; pfam00766"
/db_xref="CDD:189709"
gene 44180..45466
/gene="fixC"
/locus_tag="b0043"
/gene_synonym="ECK0044; JW0042; yaaS"
/db_xref="EcoGene:EG11564"
/db_xref="GeneID:948958"
CDS 44180..45466
/gene="fixC"
/locus_tag="b0043"
/gene_synonym="ECK0044; JW0042; yaaS"
/function="putative carrier; Energy metabolism, carbon:
Electron transport"
/GO_process="GO:0042413 - carnitine catabolic process"
/note="flavoprotein; electron transport"
/codon_start=1
/transl_table=11
/product="putative oxidoreductase with FAD/NAD(P)-binding
domain"
/protein_id="NP_414585.1"
/db_xref="GI:16128037"
/db_xref="ASAP:ABE-0000149"
/db_xref="UniProtKB/Swiss-Prot:P68644"
/db_xref="EcoGene:EG11564"
/db_xref="GeneID:948958"
/translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
misc_feature 44180..45463
/gene="fixC"
/locus_tag="b0043"
/gene_synonym="ECK0044; JW0042; yaaS"
/note="putative oxidoreductase FixC; Provisional; Region:
PRK10157"
/db_xref="CDD:182273"
gene 45463..45750
/gene="fixX"
/locus_tag="b0044"
/gene_synonym="ECK0045; JW0043; yaaT"
/db_xref="EcoGene:EG11565"
/db_xref="GeneID:948590"
CDS 45463..45750
/gene="fixX"
/locus_tag="b0044"
/gene_synonym="ECK0045; JW0043; yaaT"
/function="putative carrier; Not classified"
/GO_process="GO:0042413 - carnitine catabolic process"
/note="putative ferredoxin"
/codon_start=1
/transl_table=11
/product="putative 4Fe-4S ferredoxin-type protein"
/protein_id="NP_414586.1"
/db_xref="GI:16128038"
/db_xref="ASAP:ABE-0000151"
/db_xref="UniProtKB/Swiss-Prot:P68646"
/db_xref="EcoGene:EG11565"
/db_xref="GeneID:948590"
/translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
misc_feature 45463..45747
/gene="fixX"
/locus_tag="b0044"
/gene_synonym="ECK0045; JW0043; yaaT"
/note="ferredoxin-like protein FixX; Provisional; Region:
PRK15449"
/db_xref="CDD:185346"
gene 45807..47138
/gene="yaaU"
/locus_tag="b0045"
/gene_synonym="ECK0046; JW0044; yabE"
/db_xref="EcoGene:EG11566"
/db_xref="GeneID:944766"
CDS 45807..47138
/gene="yaaU"
/locus_tag="b0045"
/gene_synonym="ECK0046; JW0044; yabE"
/function="putative transport; Not classified"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/note="putative transport protein"
/codon_start=1
/transl_table=11
/product="putative transporter"
/protein_id="NP_414587.1"
/db_xref="GI:16128039"
/db_xref="ASAP:ABE-0000156"
/db_xref="UniProtKB/Swiss-Prot:P31679"
/db_xref="EcoGene:EG11566"
/db_xref="GeneID:944766"
/translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
misc_feature 45864..47069
/gene="yaaU"
/locus_tag="b0045"
/gene_synonym="ECK0046; JW0044; yabE"
/note="The Major Facilitator Superfamily (MFS) is a large
and diverse group of secondary transporters that includes
uniporters, symporters, and antiporters. MFS proteins
facilitate the transport across cytoplasmic or internal
membranes of a variety of...; Region: MFS; cd06174"
/db_xref="CDD:119392"
misc_feature 45882..47111
/gene="yaaU"
/locus_tag="b0045"
/gene_synonym="ECK0046; JW0044; yabE"
/note="Sugar (and other) transporter; Region: Sugar_tr;
pfam00083"
/db_xref="CDD:215702"
misc_feature order(45906..45908,45915..45923,45927..45932,45981..45983,
45990..45995,46002..46004,46014..46019,46023..46028,
46164..46169,46176..46181,46188..46193,46200..46202,
46236..46241,46248..46253,46269..46271,46572..46574,
46581..46586,46593..46598,46605..46607,46647..46649,
46659..46661,46671..46673,46680..46682,46692..46694,
46839..46841,46848..46853,46860..46862,46872..46877,
46884..46886,46917..46922,46929..46934,46941..46946,
46953..46955)
/gene="yaaU"
/locus_tag="b0045"
/gene_synonym="ECK0046; JW0044; yabE"
/note="putative substrate translocation pore; other site"
/db_xref="CDD:119392"
gene 47246..47776
/gene="kefF"
/locus_tag="b0046"
/gene_synonym="ECK0047; JW0045; yabF"
/db_xref="EcoGene:EG11568"
/db_xref="GeneID:944767"
CDS 47246..47776
/gene="kefF"
/locus_tag="b0046"
/gene_synonym="ECK0047; JW0045; yabF"
/function="putative enzyme; Not classified"
/note="putative NAD(P)H oxidoreductase"
/codon_start=1
/transl_table=11
/product="potassium-efflux system ancillary protein for
KefC, glutathione-regulated; quinone oxidoreductase,
FMN-dependent"
/protein_id="NP_414588.1"
/db_xref="GI:16128040"
/db_xref="ASAP:ABE-0000159"
/db_xref="UniProtKB/Swiss-Prot:P0A754"
/db_xref="EcoGene:EG11568"
/db_xref="GeneID:944767"
/translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
RHYKQRLLEWQEAHHG"
misc_feature 47246..47773
/gene="kefF"
/locus_tag="b0046"
/gene_synonym="ECK0047; JW0045; yabF"
/note="glutathione-regulated potassium-efflux system
ancillary protein KefF; Provisional; Region: PRK00871"
/db_xref="CDD:234852"
gene 47769..49631
/gene="kefC"
/locus_tag="b0047"
/gene_synonym="ECK0048; JW0046; trkC"
/db_xref="EcoGene:EG10521"
/db_xref="GeneID:944773"
CDS 47769..49631
/gene="kefC"
/locus_tag="b0047"
/gene_synonym="ECK0048; JW0046; trkC"
/function="transport; Transport of small molecules:
Cations"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/GO_function="GO:0015386 - potassium:hydrogen antiporter
activity"
/note="K+ efflux antiporter, glutathione-regulated"
/codon_start=1
/transl_table=11
/product="potassium:proton antiporter"
/protein_id="NP_414589.1"
/db_xref="GI:16128041"
/db_xref="ASAP:ABE-0000161"
/db_xref="UniProtKB/Swiss-Prot:P03819"
/db_xref="EcoGene:EG10521"
/db_xref="GeneID:944773"
/translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGCGALQMVICGGLLG
LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
misc_feature 47769..49628
/gene="kefC"
/locus_tag="b0047"
/gene_synonym="ECK0048; JW0046; trkC"
/note="glutathione-regulated potassium-efflux system
protein KefC; Provisional; Region: PRK03562"
/db_xref="CDD:235131"
misc_feature 47805..48623
/gene="kefC"
/locus_tag="b0047"
/gene_synonym="ECK0048; JW0046; trkC"
/note="transporter, monovalent cation:proton antiporter-2
(CPA2) family; Region: 2a37; TIGR00932"
/db_xref="CDD:233195"
misc_feature 48972..49307
/gene="kefC"
/locus_tag="b0047"
/gene_synonym="ECK0048; JW0046; trkC"
/note="TrkA-N domain; Region: TrkA_N; pfam02254"
/db_xref="CDD:216949"
gene 49823..50302
/gene="folA"
/locus_tag="b0048"
/gene_synonym="ECK0049; JW0047; tmrA"
/db_xref="EcoGene:EG10326"
/db_xref="GeneID:944790"
CDS 49823..50302
/gene="folA"
/locus_tag="b0048"
/gene_synonym="ECK0049; JW0047; tmrA"
/EC_number="1.5.1.3"
/function="enzyme; Biosynthesis of cofactors, carriers:
Folic acid"
/experiment="N-terminus verified by Edman degradation:
PMID 350268,4610415"
/GO_process="GO:0046656 - folic acid biosynthetic process"
/note="dihydrofolate reductase type I; trimethoprim
resistance"
/codon_start=1
/transl_table=11
/product="dihydrofolate reductase"
/protein_id="NP_414590.1"
/db_xref="GI:16128042"
/db_xref="ASAP:ABE-0000164"
/db_xref="UniProtKB/Swiss-Prot:P0ABQ4"
/db_xref="EcoGene:EG10326"
/db_xref="GeneID:944790"
/translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
misc_feature 49823..50299
/gene="folA"
/locus_tag="b0048"
/gene_synonym="ECK0049; JW0047; tmrA"
/note="Dihydrofolate reductase [Coenzyme metabolism];
Region: FolA; COG0262"
/db_xref="CDD:223340"
misc_feature 49826..50293
/gene="folA"
/locus_tag="b0048"
/gene_synonym="ECK0049; JW0047; tmrA"
/note="Dihydrofolate reductase (DHFR). Reduces
7,8-dihydrofolate to 5,6,7,8-tetrahydrofolate with NADPH
as a cofactor. This is an essential step in the
biosynthesis of deoxythymidine phosphate since
5,6,7,8-tetrahydrofolate is required to regenerate 5;
Region: DHFR; cd00209"
/db_xref="CDD:238127"
misc_feature order(49835..49837,49886..49888,49901..49903,49991..49993,
50102..50104,50120..50122,50159..50161)
/gene="folA"
/locus_tag="b0048"
/gene_synonym="ECK0049; JW0047; tmrA"
/note="folate binding site [chemical binding]; other site"
/db_xref="CDD:238127"
misc_feature order(49841..49843,49862..49864,49952..49960,50012..50014,
50105..50116)
/gene="folA"
/locus_tag="b0048"
/gene_synonym="ECK0049; JW0047; tmrA"
/note="NADP+ binding site [chemical binding]; other site"
/db_xref="CDD:238127"
repeat_region 50327..50363
/note="REP5 (repetitive extragenic palindromic) element;
contains 1 REP sequences"
gene complement(50380..51222)
/gene="apaH"
/locus_tag="b0049"
/gene_synonym="cfcB; ECK0050; JW0048"
/db_xref="EcoGene:EG10048"
/db_xref="GeneID:944770"
CDS complement(50380..51222)
/gene="apaH"
/locus_tag="b0049"
/gene_synonym="cfcB; ECK0050; JW0048"
/EC_number="3.6.1.41"
/function="enzyme; Salvage of nucleosides and nucleotides"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0015949 - nucleobase, nucleoside and
nucleotide interconversion"
/codon_start=1
/transl_table=11
/product="diadenosine tetraphosphatase"
/protein_id="NP_414591.1"
/db_xref="GI:16128043"
/db_xref="ASAP:ABE-0000167"
/db_xref="UniProtKB/Swiss-Prot:P05637"
/db_xref="EcoGene:EG10048"
/db_xref="GeneID:944770"
/translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
AAAS"
misc_feature complement(50386..51222)
/gene="apaH"
/locus_tag="b0049"
/gene_synonym="cfcB; ECK0050; JW0048"
/note="bis(5'-nucleosyl)-tetraphosphatase (symmetrical);
Region: apaH; TIGR00668"
/db_xref="CDD:233082"
mat_peptide complement(50383..51219)
/gene="apaH"
/locus_tag="b0049"
/gene_synonym="cfcB; ECK0050; JW0048"
/product="diadenosine tetraphosphatase"
/experiment="N-terminus verified by Edman degradation:
PMID 3031429"
misc_feature complement(50446..51216)
/gene="apaH"
/locus_tag="b0049"
/gene_synonym="cfcB; ECK0050; JW0048"
/note="Escherichia coli ApaH and related proteins,
metallophosphatase domain; Region: MPP_ApaH; cd07422"
/db_xref="CDD:163665"
misc_feature complement(order(50476..50478,50491..50493,50542..50544,
50863..50865,51025..51030,51100..51105,51112..51114,
51193..51195,51199..51201))
/gene="apaH"
/locus_tag="b0049"
/gene_synonym="cfcB; ECK0050; JW0048"
/note="active site"
/db_xref="CDD:163665"
misc_feature complement(order(50542..50544,50863..50865,51028..51030,
51112..51114,51193..51195,51199..51201))
/gene="apaH"
/locus_tag="b0049"
/gene_synonym="cfcB; ECK0050; JW0048"
/note="metal binding site [ion binding]; metal-binding
site"
/db_xref="CDD:163665"
gene complement(51229..51606)
/gene="apaG"
/locus_tag="b0050"
/gene_synonym="corD; ECK0051; JW0049; temP"
/db_xref="EcoGene:EG10047"
/db_xref="GeneID:944772"
CDS complement(51229..51606)
/gene="apaG"
/locus_tag="b0050"
/gene_synonym="corD; ECK0051; JW0049; temP"
/codon_start=1
/transl_table=11
/product="protein associated with Co2+ and Mg2+ efflux"
/protein_id="NP_414592.1"
/db_xref="GI:16128044"
/db_xref="ASAP:ABE-0000169"
/db_xref="UniProtKB/Swiss-Prot:P62672"
/db_xref="EcoGene:EG10047"
/db_xref="GeneID:944772"
/translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
DENGVPFSIDIPVFRLAVPTLIH"
misc_feature complement(51232..51606)
/gene="apaG"
/locus_tag="b0050"
/gene_synonym="corD; ECK0051; JW0049; temP"
/note="CO2+/MG2+ efflux protein ApaG; Reviewed; Region:
apaG; PRK05461"
/db_xref="CDD:180098"
gene complement(51609..52430)
/gene="rsmA"
/locus_tag="b0051"
/gene_synonym="ECK0052; JW0050; ksgA"
/db_xref="EcoGene:EG10523"
/db_xref="GeneID:944939"
CDS complement(51609..52430)
/gene="rsmA"
/locus_tag="b0051"
/gene_synonym="ECK0052; JW0050; ksgA"
/EC_number="2.1.1.-"
/function="enzyme; rRNA modification; drug/analog
sensitivity"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0009451 - RNA modification"
/GO_process="GO:0042493 - response to drug"
/codon_start=1
/transl_table=11
/product="16S rRNA m(6)A1518, m(6)A1519
dimethyltransferase, SAM-dependent"
/protein_id="NP_414593.1"
/db_xref="GI:16128045"
/db_xref="ASAP:ABE-0000175"
/db_xref="UniProtKB/Swiss-Prot:P06992"
/db_xref="EcoGene:EG10523"
/db_xref="GeneID:944939"
/translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
misc_feature complement(51630..52430)
/gene="rsmA"
/locus_tag="b0051"
/gene_synonym="ECK0052; JW0050; ksgA"
/note="16S ribosomal RNA methyltransferase KsgA/Dim1
family protein; Reviewed; Region: ksgA; PRK00274"
/db_xref="CDD:234708"
misc_feature complement(51624..52400)
/gene="rsmA"
/locus_tag="b0051"
/gene_synonym="ECK0052; JW0050; ksgA"
/note="Dimethyladenosine transferase (rRNA methylation)
[Translation, ribosomal structure and biogenesis]; Region:
KsgA; COG0030"
/db_xref="CDD:223109"
gene complement(52427..53416)
/gene="pdxA"
/locus_tag="b0052"
/gene_synonym="ECK0053; JW0051"
/db_xref="EcoGene:EG10691"
/db_xref="GeneID:944919"
CDS complement(52427..53416)
/gene="pdxA"
/locus_tag="b0052"
/gene_synonym="ECK0053; JW0051"
/EC_number="1.1.1.262"
/function="enzyme; Biosynthesis of cofactors, carriers:
Pyridoxine"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0008615 - pyridoxine biosynthetic process"
/note="pyridoxine biosynthesis"
/codon_start=1
/transl_table=11
/product="4-hydroxy-L-threonine phosphate dehydrogenase,
NAD-dependent"
/protein_id="NP_414594.1"
/db_xref="GI:16128046"
/db_xref="ASAP:ABE-0000178"
/db_xref="UniProtKB/Swiss-Prot:P19624"
/db_xref="EcoGene:EG10691"
/db_xref="GeneID:944919"
/translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
NRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRAPVTAGQLAVENGHYVVETLA
RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
misc_feature complement(52430..53416)
/gene="pdxA"
/locus_tag="b0052"
/gene_synonym="ECK0053; JW0051"
/note="4-hydroxythreonine-4-phosphate dehydrogenase;
Reviewed; Region: pdxA; PRK00232"
/db_xref="CDD:234696"
misc_feature complement(52448..53398)
/gene="pdxA"
/locus_tag="b0052"
/gene_synonym="ECK0053; JW0051"
/note="4-hydroxythreonine-4-phosphate dehydrogenase;
Validated; Region: pdxA; PRK03743"
/db_xref="CDD:179641"
gene complement(53416..54702)
/gene="surA"
/locus_tag="b0053"
/gene_synonym="ECK0054; JW0052"
/db_xref="EcoGene:EG10985"
/db_xref="GeneID:944812"
CDS complement(53416..54702)
/gene="surA"
/locus_tag="b0053"
/gene_synonym="ECK0054; JW0052"
/EC_number="5.2.1.8"
/function="phenotype; Not classified"
/GO_component="GO:0009279 - cell outer membrane"
/GO_process="GO:0006457 - protein folding"
/note="survival protein"
/codon_start=1
/transl_table=11
/product="peptidyl-prolyl cis-trans isomerase (PPIase)"
/protein_id="NP_414595.1"
/db_xref="GI:16128047"
/db_xref="ASAP:ABE-0000180"
/db_xref="UniProtKB/Swiss-Prot:P0ABZ6"
/db_xref="EcoGene:EG10985"
/db_xref="GeneID:944812"
/translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
misc_feature complement(53419..54648)
/gene="surA"
/locus_tag="b0053"
/gene_synonym="ECK0054; JW0052"
/note="peptidyl-prolyl cis-trans isomerase SurA;
Provisional; Region: PRK10770"
/db_xref="CDD:236758"
mat_peptide complement(53419..54642)
/gene="surA"
/locus_tag="b0053"
/gene_synonym="ECK0054; JW0052"
/product="peptidyl-prolyl cis-trans isomerase (PPIase)"
/experiment="N-terminus verified by Edman degradation:
PMID 9298646,9600841"
misc_feature complement(54277..54630)
/gene="surA"
/locus_tag="b0053"
/gene_synonym="ECK0054; JW0052"
/note="SurA N-terminal domain; Region: SurA_N; pfam09312"
/db_xref="CDD:150092"
misc_feature complement(53887..54171)
/gene="surA"
/locus_tag="b0053"
/gene_synonym="ECK0054; JW0052"
/note="PPIC-type PPIASE domain; Region: Rotamase;
pfam00639"
/db_xref="CDD:216038"
misc_feature complement(53557..53838)
/gene="surA"
/locus_tag="b0053"
/gene_synonym="ECK0054; JW0052"
/note="PPIC-type PPIASE domain; Region: Rotamase;
pfam00639"
/db_xref="CDD:216038"
gene complement(54755..57109)
/gene="lptD"
/locus_tag="b0054"
/gene_synonym="ECK0055; imp; JW0053; ostA; yabG"
/db_xref="EcoGene:EG11569"
/db_xref="GeneID:945011"
CDS complement(54755..57109)
/gene="lptD"
/locus_tag="b0054"
/gene_synonym="ECK0055; imp; JW0053; ostA; yabG"
/function="phenotype; Adaptations, atypical conditions"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/note="organic solvent tolerance"
/codon_start=1
/transl_table=11
/product="LPS assembly OM complex LptDE, beta-barrel
component"
/protein_id="NP_414596.1"
/db_xref="GI:16128048"
/db_xref="ASAP:ABE-0000183"
/db_xref="UniProtKB/Swiss-Prot:P31554"
/db_xref="EcoGene:EG11569"
/db_xref="GeneID:945011"
/translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
misc_feature complement(54764..57109)
/gene="lptD"
/locus_tag="b0054"
/gene_synonym="ECK0055; imp; JW0053; ostA; yabG"
/note="LPS assembly outer membrane complex protein LptD;
Provisional; Region: PRK03761"
/db_xref="CDD:235158"
mat_peptide complement(54758..57037)
/gene="lptD"
/locus_tag="b0054"
/gene_synonym="ECK0055; imp; JW0053; ostA; yabG"
/product="LPS assembly OM complex LptDE, beta-barrel
component"
/experiment="N-terminus verified by Edman degradation:
PMID 9298646"
misc_feature complement(56525..56953)
/gene="lptD"
/locus_tag="b0054"
/gene_synonym="ECK0055; imp; JW0053; ostA; yabG"
/note="OstA-like protein; Region: OstA; pfam03968"
/db_xref="CDD:217820"
misc_feature complement(55019..56182)
/gene="lptD"
/locus_tag="b0054"
/gene_synonym="ECK0055; imp; JW0053; ostA; yabG"
/note="Organic solvent tolerance protein; Region: OstA_C;
pfam04453"
/db_xref="CDD:218093"
gene 57364..58179
/gene="djlA"
/locus_tag="b0055"
/gene_synonym="ECK0056; JW0054; yabH"
/db_xref="EcoGene:EG11570"
/db_xref="GeneID:948992"
CDS 57364..58179
/gene="djlA"
/locus_tag="b0055"
/gene_synonym="ECK0056; JW0054; yabH"
/function="phenotype; Not classified"
/GO_process="GO:0006457 - protein folding"
/codon_start=1
/transl_table=11
/product="DnaJ-like protein, membrane anchored"
/protein_id="NP_414597.1"
/db_xref="GI:16128049"
/db_xref="ASAP:ABE-0000187"
/db_xref="UniProtKB/Swiss-Prot:P31680"
/db_xref="EcoGene:EG11570"
/db_xref="GeneID:948992"
/translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
LGISRAQFDQFLRMMQGGAQFGGGYQQQTGGGNWQQAQRGPTLEDACNVLGVKPTDDA
TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
misc_feature 57364..58176
/gene="djlA"
/locus_tag="b0055"
/gene_synonym="ECK0056; JW0054; yabH"
/note="Dna-J like membrane chaperone protein; Provisional;
Region: djlA; PRK09430"
/db_xref="CDD:236512"
misc_feature 57532..57849
/gene="djlA"
/locus_tag="b0055"
/gene_synonym="ECK0056; JW0054; yabH"
/note="N-terminal tellurium resistance protein terB-like
domain of heat shock DnaJ-like proteins; Region:
terB_like_DjlA; cd07316"
/db_xref="CDD:143585"
misc_feature order(57568..57570,57589..57591,57790..57792,57811..57813)
/gene="djlA"
/locus_tag="b0055"
/gene_synonym="ECK0056; JW0054; yabH"
/note="putative metal binding site [ion binding]; other
site"
/db_xref="CDD:143585"
misc_feature 57988..58149
/gene="djlA"
/locus_tag="b0055"
/gene_synonym="ECK0056; JW0054; yabH"
/note="DnaJ domain or J-domain. DnaJ/Hsp40 (heat shock
protein 40) proteins are highly conserved and play crucial
roles in protein translation, folding, unfolding,
translocation, and degradation. They act primarily by
stimulating the ATPase activity of Hsp70s; Region: DnaJ;
cd06257"
/db_xref="CDD:99751"
misc_feature order(58060..58068,58114..58116,58123..58128,58135..58140)
/gene="djlA"
/locus_tag="b0055"
/gene_synonym="ECK0056; JW0054; yabH"
/note="HSP70 interaction site [polypeptide binding]; other
site"
/db_xref="CDD:99751"
gene 58474..59269
/gene="yabP"
/locus_tag="b4659"
/gene_synonym="ECK0057; JW0055; yabQ"
/pseudo
/db_xref="EcoGene:EG12610"
/db_xref="GeneID:944776"
CDS join(58474..59052,59052..59228,59228..59269)
/gene="yabP"
/locus_tag="b4659"
/gene_synonym="ECK0057; JW0055; yabQ"
/note="pseudogene"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="ASAP:ABE-0000192"
/db_xref="ASAP:ABE-0000194"
/db_xref="UniProtKB/Swiss-Prot:P39220"
/db_xref="EcoGene:EG12610"
/db_xref="GeneID:944776"
gene complement(59687..60346)
/gene="rluA"
/locus_tag="b0058"
/gene_synonym="ECK0059; JW0057; yabO"
/db_xref="EcoGene:EG12609"
/db_xref="GeneID:946262"
CDS complement(59687..60346)
/gene="rluA"
/locus_tag="b0058"
/gene_synonym="ECK0059; JW0057; yabO"
/EC_number="4.2.1.70"
/function="enzyme; rRNA modification; tRNA modification"
/GO_process="GO:0009451 - RNA modification"
/codon_start=1
/transl_table=11
/product="dual specificity 23S rRNA pseudouridine(746),
tRNA pseudouridine(32) synthase, SAM-dependent"
/protein_id="NP_414600.1"
/db_xref="GI:16128052"
/db_xref="ASAP:ABE-0000196"
/db_xref="UniProtKB/Swiss-Prot:P0AA37"
/db_xref="EcoGene:EG12609"
/db_xref="GeneID:946262"
/translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
F"
mat_peptide complement(59690..60343)
/gene="rluA"
/locus_tag="b0058"
/gene_synonym="ECK0059; JW0057; yabO"
/product="dual specificity 23S rRNA pseudouridine(746),
tRNA pseudouridine(32) synthase, SAM-dependent"
/experiment="N-terminus verified by Edman degradation:
PMID 7493321"
misc_feature complement(59690..>60322)
/gene="rluA"
/locus_tag="b0058"
/gene_synonym="ECK0059; JW0057; yabO"
/note="Pseudouridylate synthases, 23S RNA-specific
[Translation, ribosomal structure and biogenesis]; Region:
RluA; COG0564"
/db_xref="CDD:223638"
misc_feature complement(59741..60280)
/gene="rluA"
/locus_tag="b0058"
/gene_synonym="ECK0059; JW0057; yabO"
/note="Pseudouridine synthase, RsuA/RluD family; Region:
PseudoU_synth_RluCD_like; cd02869"
/db_xref="CDD:211346"
misc_feature complement(order(59852..59854,60155..60166))
/gene="rluA"
/locus_tag="b0058"
/gene_synonym="ECK0059; JW0057; yabO"
/note="active site"
/db_xref="CDD:211346"
gene complement(60358..63264)
/gene="rapA"
/locus_tag="b0059"
/gene_synonym="ECK0060; hepA; JW0058; yabA"
/db_xref="EcoGene:EG11083"
/db_xref="GeneID:948523"
CDS complement(60358..63264)
/gene="rapA"
/locus_tag="b0059"
/gene_synonym="ECK0060; hepA; JW0058; yabA"
/EC_number="3.6.1.-"
/function="putative enzyme; RNA synthesis, modification,
DNA transcription"
/GO_process="GO:0009451 - RNA modification"
/note="probable ATP-dependent RNA helicase"
/codon_start=1
/transl_table=11
/product="RNA polymerase-associated helicase protein
(ATPase and RNA polymerase recycling factor)"
/protein_id="NP_414601.1"
/db_xref="GI:16128053"
/db_xref="ASAP:ABE-0000198"
/db_xref="UniProtKB/Swiss-Prot:P60240"
/db_xref="EcoGene:EG11083"
/db_xref="GeneID:948523"
/translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
mat_peptide complement(60361..63261)
/gene="rapA"
/locus_tag="b0059"
/gene_synonym="ECK0060; hepA; JW0058; yabA"
/product="RNA polymerase-associated helicase protein
(ATPase and RNA polymerase recycling factor)"
/experiment="N-terminus verified by Edman degradation:
PMID 9614128,11751638"
misc_feature complement(60367..63261)
/gene="rapA"
/locus_tag="b0059"
/gene_synonym="ECK0060; hepA; JW0058; yabA"
/note="ATP-dependent helicase HepA; Validated; Region:
PRK04914"
/db_xref="CDD:235319"
misc_feature complement(62317..62748)
/gene="rapA"
/locus_tag="b0059"
/gene_synonym="ECK0060; hepA; JW0058; yabA"
/note="DEAD-like helicases superfamily. A diverse family
of proteins involved in ATP-dependent RNA or DNA
unwinding. This domain contains the ATP-binding region;
Region: DEXDc; cd00046"
/db_xref="CDD:238005"
misc_feature complement(62713..62727)
/gene="rapA"
/locus_tag="b0059"
/gene_synonym="ECK0060; hepA; JW0058; yabA"
/note="ATP binding site [chemical binding]; other site"
/db_xref="CDD:238005"
misc_feature complement(62416..62427)
/gene="rapA"
/locus_tag="b0059"
/gene_synonym="ECK0060; hepA; JW0058; yabA"
/note="putative Mg++ binding site [ion binding]; other
site"
/db_xref="CDD:238005"
misc_feature complement(61459..61827)
/gene="rapA"
/locus_tag="b0059"
/gene_synonym="ECK0060; hepA; JW0058; yabA"
/note="Helicase superfamily c-terminal domain; associated
with DEXDc-, DEAD-, and DEAH-box proteins, yeast
initiation factor 4A, Ski2p, and Hepatitis C virus NS3
helicases; this domain is found in a wide variety of
helicases and helicase related proteins; may...; Region:
HELICc; cd00079"
/db_xref="CDD:238034"
misc_feature complement(order(61570..61578,61657..61662,61723..61734))
/gene="rapA"
/locus_tag="b0059"
/gene_synonym="ECK0060; hepA; JW0058; yabA"
/note="nucleotide binding region [chemical binding]; other
site"
/db_xref="CDD:238034"
misc_feature complement(order(61468..61470,61477..61479,61489..61491,
61552..61554))
/gene="rapA"
/locus_tag="b0059"
/gene_synonym="ECK0060; hepA; JW0058; yabA"
/note="ATP-binding site [chemical binding]; other site"
/db_xref="CDD:238034"
gene complement(63429..65780)
/gene="polB"
/locus_tag="b0060"
/gene_synonym="dinA; ECK0061; JW0059"
/db_xref="EcoGene:EG10747"
/db_xref="GeneID:944779"
CDS complement(63429..65780)
/gene="polB"
/locus_tag="b0060"
/gene_synonym="dinA; ECK0061; JW0059"
/EC_number="2.7.7.7"
/function="enzyme; DNA - replication, repair,
restriction/modification"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006261 - DNA-dependent DNA replication"
/GO_process="GO:0006281 - DNA repair"
/GO_process="GO:0009314 - response to radiation"
/GO_process="GO:0009432 - SOS response"
/codon_start=1
/transl_table=11
/product="DNA polymerase II"
/protein_id="NP_414602.1"
/db_xref="GI:16128054"
/db_xref="ASAP:ABE-0000202"
/db_xref="UniProtKB/Swiss-Prot:P21189"
/db_xref="EcoGene:EG10747"
/db_xref="GeneID:944779"
/translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
misc_feature complement(63432..65780)
/gene="polB"
/locus_tag="b0060"
/gene_synonym="dinA; ECK0061; JW0059"
/note="DNA polymerase II; Reviewed; Region: PRK05762"
/db_xref="CDD:235595"
mat_peptide complement(63432..65777)
/gene="polB"
/locus_tag="b0060"
/gene_synonym="dinA; ECK0061; JW0059"
/product="DNA polymerase II"
/experiment="N-terminus verified by Edman degradation:
PMID 2034216,2217198"
misc_feature complement(64749..65339)
/gene="polB"
/locus_tag="b0060"
/gene_synonym="dinA; ECK0061; JW0059"
/note="DEDDy 3'-5' exonuclease domain of Escherichia coli
DNA polymerase II and similar bacterial family-B DNA
polymerases; Region: DNA_polB_II_exo; cd05784"
/db_xref="CDD:99827"
misc_feature complement(order(64776..64778,64788..64790,64902..64907,
65094..65102,65109..65114,65304..65315))
/gene="polB"
/locus_tag="b0060"
/gene_synonym="dinA; ECK0061; JW0059"
/note="active site"
/db_xref="CDD:99827"
misc_feature complement(order(64776..64778,64788..64790,65094..65096,
65307..65309,65313..65315))
/gene="polB"
/locus_tag="b0060"
/gene_synonym="dinA; ECK0061; JW0059"
/note="catalytic site [active]"
/db_xref="CDD:99827"
misc_feature complement(order(64776..64778,64788..64790,64902..64907,
65097..65102,65109..65114,65304..65312))
/gene="polB"
/locus_tag="b0060"
/gene_synonym="dinA; ECK0061; JW0059"
/note="substrate binding site [chemical binding]; other
site"
/db_xref="CDD:99827"
misc_feature complement(63474..64592)
/gene="polB"
/locus_tag="b0060"
/gene_synonym="dinA; ECK0061; JW0059"
/note="DNA polymerase type-II subfamily catalytic domain.
Bacteria contain five DNA polymerases (I, II, III, IV and
V). DNA polymerase II (Pol II) is a prototype for the
B-family of polymerases. The role of Pol II in a variety
of cellular activities, such as...; Region: POLBc_Pol_II;
cd05537"
/db_xref="CDD:99920"
misc_feature complement(order(64140..64142,64290..64292,64302..64304,
64350..64352,64509..64517,64524..64526))
/gene="polB"
/locus_tag="b0060"
/gene_synonym="dinA; ECK0061; JW0059"
/note="active site"
/db_xref="CDD:99920"
misc_feature complement(order(64140..64142,64146..64148))
/gene="polB"
/locus_tag="b0060"
/gene_synonym="dinA; ECK0061; JW0059"
/note="metal-binding site"
/db_xref="CDD:99920"
gene complement(65855..66550)
/gene="araD"
/locus_tag="b0061"
/gene_synonym="ECK0062; JW0060"
/db_xref="EcoGene:EG10055"
/db_xref="GeneID:945294"
CDS complement(65855..66550)
/gene="araD"
/locus_tag="b0061"
/gene_synonym="ECK0062; JW0060"
/EC_number="5.1.3.4"
/function="enzyme; Degradation of small molecules: Carbon
compounds"
/experiment="N-terminus verified by Edman degradation:
PMID 3549454,8520491"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0016052 - carbohydrate catabolic process"
/codon_start=1
/transl_table=11
/product="L-ribulose-5-phosphate 4-epimerase"
/protein_id="NP_414603.1"
/db_xref="GI:16128055"
/db_xref="ASAP:ABE-0000209"
/db_xref="UniProtKB/Swiss-Prot:P08203"
/db_xref="EcoGene:EG10055"
/db_xref="GeneID:945294"
/translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
YLRKHGAKAYYGQ"
misc_feature complement(65858..66550)
/gene="araD"
/locus_tag="b0061"
/gene_synonym="ECK0062; JW0060"
/note="L-ribulose-5-phosphate 4-epimerase; Reviewed;
Region: araD; PRK08193"
/db_xref="CDD:236181"
misc_feature complement(65882..66544)
/gene="araD"
/locus_tag="b0061"
/gene_synonym="ECK0062; JW0060"
/note="Class II Aldolase and Adducin head (N-terminal)
domain. Aldolases are ubiquitous enzymes catalyzing
central steps of carbohydrate metabolism. Based on
enzymatic mechanisms, this superfamily has been divided
into two distinct classes (Class I and II); Region:
Aldolase_II; cd00398"
/db_xref="CDD:238232"
misc_feature complement(order(65894..65896,65954..65956,65975..65977,
65987..65989,66008..66010,66041..66043,66158..66160,
66200..66205,66209..66211,66230..66238,66251..66256,
66260..66262,66401..66406,66410..66412,66482..66484,
66506..66508,66518..66520))
/gene="araD"
/locus_tag="b0061"
/gene_synonym="ECK0062; JW0060"
/note="intersubunit interface [polypeptide binding]; other
site"
/db_xref="CDD:238232"
misc_feature complement(order(66038..66040,66260..66262,66266..66268,
66323..66331,66416..66421,66467..66469))
/gene="araD"
/locus_tag="b0061"
/gene_synonym="ECK0062; JW0060"
/note="active site"
/db_xref="CDD:238232"
misc_feature complement(order(66038..66040,66260..66262,66266..66268))
/gene="araD"
/locus_tag="b0061"
/gene_synonym="ECK0062; JW0060"
/note="Zn2+ binding site [ion binding]; other site"
/db_xref="CDD:238232"
repeat_region 66565..66812
/note="REP6 (repetitive extragenic palindromic) element;
contains 6 REP sequences"
gene complement(66835..68337)
/gene="araA"
/locus_tag="b0062"
/gene_synonym="ECK0063; JW0061"
/db_xref="EcoGene:EG10052"
/db_xref="GeneID:947511"
CDS complement(66835..68337)
/gene="araA"
/locus_tag="b0062"
/gene_synonym="ECK0063; JW0061"
/EC_number="5.3.1.4"
/function="enzyme; Degradation of small molecules: Carbon
compounds"
/experiment="N-terminus verified by Edman degradation:
PMID 3549454"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0016052 - carbohydrate catabolic process"
/codon_start=1
/transl_table=11
/product="L-arabinose isomerase"
/protein_id="NP_414604.1"
/db_xref="GI:16128056"
/db_xref="ASAP:ABE-0000221"
/db_xref="UniProtKB/Swiss-Prot:P08202"
/db_xref="EcoGene:EG10052"
/db_xref="GeneID:947511"
/translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
NSISDGDVNALVDEYESCYTMTPATQIHGKKRQNVLEAARIELGMKRFLEQGGFHAFT
TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
misc_feature complement(66838..68337)
/gene="araA"
/locus_tag="b0062"
/gene_synonym="ECK0063; JW0061"
/note="L-arabinose isomerase; Provisional; Region:
PRK02929"
/db_xref="CDD:179503"
misc_feature complement(66865..68319)
/gene="araA"
/locus_tag="b0062"
/gene_synonym="ECK0063; JW0061"
/note="L-Arabinose isomerase (AI) catalyzes the
isomerization of L-arabinose to L-ribulose, the first
reaction in its conversion into D-xylulose-5-phosphate, an
intermediate in the pentose phosphate pathway, which
allows L-arabinose to be used as a carbon...; Region:
L-arabinose_isomerase; cd03557"
/db_xref="CDD:239619"
misc_feature complement(order(67495..67500,67525..67527,67690..67692,
67702..67722,67741..67743,67750..67752,67786..67788,
67807..67809,67915..67920,68074..68076,68134..68136,
68143..68145,68155..68157))
/gene="araA"
/locus_tag="b0062"
/gene_synonym="ECK0063; JW0061"
/note="putative hexamer (dimer of trimers) interface
[polypeptide binding]; other site"
/db_xref="CDD:239619"
misc_feature complement(order(66865..66870,66937..66939,66991..66993,
67000..67002,67009..67011,67018..67023,67066..67068,
67072..67077,67081..67083,67138..67146,67321..67338,
67768..67779,67861..67863,67894..67896,67900..67902,
67915..67917,67921..67926,67933..67935,67942..67947,
67954..67959,67975..67977,67984..67992,67999..68001,
68005..68007,68011..68013,68020..68022,68026..68028,
68074..68085,68089..68091))
/gene="araA"
/locus_tag="b0062"
/gene_synonym="ECK0063; JW0061"
/note="trimer interface [polypeptide binding]; other site"
/db_xref="CDD:239619"
misc_feature complement(order(66988..66993,67339..67341,67420..67422,
67501..67503,68089..68091))
/gene="araA"
/locus_tag="b0062"
/gene_synonym="ECK0063; JW0061"
/note="putative substrate binding site [chemical binding];
other site"
/db_xref="CDD:239619"
misc_feature complement(order(66988..66990,67339..67341,67420..67422))
/gene="araA"
/locus_tag="b0062"
/gene_synonym="ECK0063; JW0061"
/note="putative metal binding site [ion binding]; other
site"
/db_xref="CDD:239619"
gene complement(68348..70048)
/gene="araB"
/locus_tag="b0063"
/gene_synonym="ECK0064; JW0062"
/db_xref="EcoGene:EG10053"
/db_xref="GeneID:946017"
CDS complement(68348..70048)
/gene="araB"
/locus_tag="b0063"
/gene_synonym="ECK0064; JW0062"
/EC_number="2.7.1.16"
/function="enzyme; Degradation of small molecules: Carbon
compounds"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0016052 - carbohydrate catabolic process"
/codon_start=1
/transl_table=11
/product="L-ribulokinase"
/protein_id="NP_414605.1"
/db_xref="GI:16128057"
/db_xref="ASAP:ABE-0000223"
/db_xref="UniProtKB/Swiss-Prot:P08204"
/db_xref="EcoGene:EG10053"
/db_xref="GeneID:946017"
/translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQKGQFCD
APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
QSAFGDIYAWFGRVLGWPLEQLAAQHPELKTQINASQKQLLPALTEAWAKNPSLDHLP
VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
"
misc_feature complement(68390..70048)
/gene="araB"
/locus_tag="b0063"
/gene_synonym="ECK0064; JW0062"
/note="ribulokinase; Provisional; Region: PRK04123"
/db_xref="CDD:235221"
mat_peptide complement(68351..70045)
/gene="araB"
/locus_tag="b0063"
/gene_synonym="ECK0064; JW0062"
/product="L-ribulokinase"
/experiment="N-terminus verified by Edman degradation:
PMID 189315"
misc_feature complement(68507..70045)
/gene="araB"
/locus_tag="b0063"
/gene_synonym="ECK0064; JW0062"
/note="Ribulokinases; belongs to the FGGY family of
carbohydrate kinases; Region: FGGY_RBK; cd07781"
/db_xref="CDD:198358"
misc_feature complement(order(68507..68512,68546..68563,68567..68572,
68576..68584,68588..68590,68831..68833,68843..68845,
69053..69061,69071..69073,69077..69103,69110..69112,
69116..69118,69206..69214,69218..69220,69242..69244,
69248..69250,69305..69319,69413..69421,69431..69436,
69443..69448,69584..69586,69599..69601,69605..69616,
69791..69808,69962..69964,69974..69979,69992..69994,
69998..70000,70004..70006,70025..70027,70031..70033,
70037..70039))
/gene="araB"
/locus_tag="b0063"
/gene_synonym="ECK0064; JW0062"
/note="N- and C-terminal domain interface [polypeptide
binding]; other site"
/db_xref="CDD:198358"
misc_feature complement(order(68582..68584,68654..68656,68666..68674,
68915..68917,69020..69022,69032..69034,69155..69157,
69164..69172,69224..69229,69692..69694,69779..69781,
69785..69787,70004..70006,70010..70021,70025..70027))
/gene="araB"
/locus_tag="b0063"
/gene_synonym="ECK0064; JW0062"
/note="active site"
/db_xref="CDD:198358"
misc_feature complement(order(68654..68656,68666..68674,68906..68908,
68912..68917,69020..69025,69029..69034,69164..69172,
69227..69229,70004..70006,70010..70021,70025..70027))
/gene="araB"
/locus_tag="b0063"
/gene_synonym="ECK0064; JW0062"
/note="MgATP binding site [chemical binding]; other site"
/db_xref="CDD:198358"
misc_feature complement(order(69227..69229,70016..70018,70025..70027))
/gene="araB"
/locus_tag="b0063"
/gene_synonym="ECK0064; JW0062"
/note="catalytic site [active]"
/db_xref="CDD:198358"
misc_feature complement(order(69227..69229,70025..70027))
/gene="araB"
/locus_tag="b0063"
/gene_synonym="ECK0064; JW0062"
/note="metal binding site [ion binding]; metal-binding
site"
/db_xref="CDD:198358"
misc_feature complement(order(69053..69055,69155..69157,69164..69166,
69224..69229,69428..69430,69587..69589,69692..69694,
69779..69787,70016..70021))
/gene="araB"
/locus_tag="b0063"
/gene_synonym="ECK0064; JW0062"
/note="carbohydrate binding site [chemical binding]; other
site"
/db_xref="CDD:198358"
misc_feature complement(order(68780..68788,68792..68818,68852..68857,
68870..68872,68876..68878,68993..69010,69017..69019,
69029..69031))
/gene="araB"
/locus_tag="b0063"
/gene_synonym="ECK0064; JW0062"
/note="homodimer interface [polypeptide binding]; other
site"
/db_xref="CDD:198358"
gene 70387..71265
/gene="araC"
/locus_tag="b0064"
/gene_synonym="ECK0065; JW0063"
/db_xref="EcoGene:EG10054"
/db_xref="GeneID:944780"
CDS 70387..71265
/gene="araC"
/locus_tag="b0064"
/gene_synonym="ECK0065; JW0063"
/function="regulator; Degradation of small molecules:
Carbon compounds"
/GO_component="GO:0005737 - cytoplasm"
/GO_function="GO:0016563 - transcription activator
activity"
/GO_function="GO:0016564 - transcription repressor
activity"
/GO_process="GO:0016052 - carbohydrate catabolic process"
/GO_process="GO:0006350 - transcription"
/note="transcriptional regulator for ara operon"
/codon_start=1
/transl_table=11
/product="DNA-binding transcriptional dual regulator"
/protein_id="NP_414606.1"
/db_xref="GI:16128058"
/db_xref="ASAP:ABE-0000236"
/db_xref="UniProtKB/Swiss-Prot:P0A9E0"
/db_xref="EcoGene:EG10054"
/db_xref="GeneID:944780"
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
RAGCEEKVNDVAVKLS"
misc_feature 70387..71241
/gene="araC"
/locus_tag="b0064"
/gene_synonym="ECK0065; JW0063"
/note="DNA-binding transcriptional regulator AraC;
Provisional; Region: PRK10572"
/db_xref="CDD:236717"
mat_peptide 70390..71262
/gene="araC"
/locus_tag="b0064"
/gene_synonym="ECK0065; JW0063"
/product="DNA-binding transcriptional dual regulator"
/experiment="N-terminus verified by Edman degradation:
PMID 7019009"
misc_feature 70447..70833
/gene="araC"
/locus_tag="b0064"
/gene_synonym="ECK0065; JW0063"
/note="AraC-like ligand binding domain; Region:
AraC_binding; pfam02311"
/db_xref="CDD:145456"
misc_feature 70942..71067
/gene="araC"
/locus_tag="b0064"
/gene_synonym="ECK0065; JW0063"
/note="Bacterial regulatory helix-turn-helix proteins,
AraC family; Region: HTH_AraC; pfam00165"
/db_xref="CDD:215763"
misc_feature 71107..71217
/gene="araC"
/locus_tag="b0064"
/gene_synonym="ECK0065; JW0063"
/note="Bacterial regulatory helix-turn-helix proteins,
AraC family; Region: HTH_AraC; pfam00165"
/db_xref="CDD:215763"
gene 71351..72115
/gene="yabI"
/locus_tag="b0065"
/gene_synonym="ECK0066; JW5005"
/db_xref="EcoGene:EG11571"
/db_xref="GeneID:944783"
CDS 71351..72115
/gene="yabI"
/locus_tag="b0065"
/gene_synonym="ECK0066; JW5005"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/codon_start=1
/transl_table=11
/product="inner membrane protein, SNARE_assoc family"
/protein_id="NP_414607.1"
/db_xref="GI:16128059"
/db_xref="ASAP:ABE-0000241"
/db_xref="UniProtKB/Swiss-Prot:P30149"
/db_xref="EcoGene:EG11571"
/db_xref="GeneID:944783"
/translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
LGALIGSGELSFWHAWLAGIIGCLMGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
misc_feature 71372..71968
/gene="yabI"
/locus_tag="b0065"
/gene_synonym="ECK0066; JW5005"
/note="Uncharacterized membrane-associated protein
[Function unknown]; Region: DedA; COG0586"
/db_xref="CDD:223659"
misc_feature 71456..71833
/gene="yabI"
/locus_tag="b0065"
/gene_synonym="ECK0066; JW5005"
/note="SNARE associated Golgi protein; Region:
SNARE_assoc; pfam09335"
/db_xref="CDD:220186"
repeat_region 72136..72220
/note="REP7 (repetitive extragenic palindromic) element;
contains 2 REP sequences"
gene complement(72229..72927)
/gene="thiQ"
/locus_tag="b0066"
/gene_synonym="ECK0067; JW0065; sfuC; yabJ"
/db_xref="EcoGene:EG11572"
/db_xref="GeneID:944785"
CDS complement(72229..72927)
/gene="thiQ"
/locus_tag="b0066"
/gene_synonym="ECK0067; JW0065; sfuC; yabJ"
/function="putative transport; Not classified"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0009228 - thiamin biosynthetic process"
/note="putative ATP-binding component of a transport
system"
/codon_start=1
/transl_table=11
/product="thiamin transporter subunit"
/protein_id="NP_414608.1"
/db_xref="GI:16128060"
/db_xref="ASAP:ABE-0000244"
/db_xref="UniProtKB/Swiss-Prot:P31548"
/db_xref="EcoGene:EG11572"
/db_xref="GeneID:944785"
/translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
IAGFLTPASGSLTIDGVDHTTMPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
NAVQQGKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTNELL
SGKASASALLGITG"
misc_feature complement(72280..72927)
/gene="thiQ"
/locus_tag="b0066"
/gene_synonym="ECK0067; JW0065; sfuC; yabJ"
/note="thiamine transporter ATP-binding subunit;
Provisional; Region: thiQ; PRK10771"
/db_xref="CDD:182716"
misc_feature complement(72292..72924)
/gene="thiQ"
/locus_tag="b0066"
/gene_synonym="ECK0067; JW0065; sfuC; yabJ"
/note="ATP-binding cassette domain of the thiamine
transport system; Region: ABC_ThiQ_thiamine_transporter;
cd03298"
/db_xref="CDD:213265"
misc_feature complement(72811..72834)
/gene="thiQ"
/locus_tag="b0066"
/gene_synonym="ECK0067; JW0065; sfuC; yabJ"
/note="Walker A/P-loop; other site"
/db_xref="CDD:213265"
misc_feature complement(order(72364..72366,72463..72468,72694..72696,
72808..72816,72820..72825))
/gene="thiQ"
/locus_tag="b0066"
/gene_synonym="ECK0067; JW0065; sfuC; yabJ"
/note="ATP binding site [chemical binding]; other site"
/db_xref="CDD:213265"
misc_feature complement(72694..72705)
/gene="thiQ"
/locus_tag="b0066"
/gene_synonym="ECK0067; JW0065; sfuC; yabJ"
/note="Q-loop/lid; other site"
/db_xref="CDD:213265"
misc_feature complement(72511..72540)
/gene="thiQ"
/locus_tag="b0066"
/gene_synonym="ECK0067; JW0065; sfuC; yabJ"
/note="ABC transporter signature motif; other site"
/db_xref="CDD:213265"
misc_feature complement(72463..72480)
/gene="thiQ"
/locus_tag="b0066"
/gene_synonym="ECK0067; JW0065; sfuC; yabJ"
/note="Walker B; other site"
/db_xref="CDD:213265"
misc_feature complement(72445..72456)
/gene="thiQ"
/locus_tag="b0066"
/gene_synonym="ECK0067; JW0065; sfuC; yabJ"
/note="D-loop; other site"
/db_xref="CDD:213265"
misc_feature complement(72358..72378)
/gene="thiQ"
/locus_tag="b0066"
/gene_synonym="ECK0067; JW0065; sfuC; yabJ"
/note="H-loop/switch region; other site"
/db_xref="CDD:213265"
gene complement(72911..74521)
/gene="thiP"
/locus_tag="b0067"
/gene_synonym="ECK0068; JW0066; sfuB; yabK"
/db_xref="EcoGene:EG11573"
/db_xref="GeneID:944784"
CDS complement(72911..74521)
/gene="thiP"
/locus_tag="b0067"
/gene_synonym="ECK0068; JW0066; sfuB; yabK"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/GO_process="GO:0009228 - thiamin biosynthetic process"
/codon_start=1
/transl_table=11
/product="fused thiamin transporter subunits of ABC
superfamily: membrane components"
/protein_id="NP_414609.1"
/db_xref="GI:16128061"
/db_xref="ASAP:ABE-0000246"
/db_xref="UniProtKB/Swiss-Prot:P31549"
/db_xref="EcoGene:EG11573"
/db_xref="GeneID:944784"
/translation="MATRRQPLIPGWLIPGVSATTLVVAVALAAFLALWWNAPQDDWV
AVWQDSYLWHVVRFSFWQAFLSALLSVIPAIFLARALYRRRFPGRLALLRLCAMTLIL
PVLVAVFGILSVYGRQGWLATLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
ALENIPGEQRQLAAQLGMRSWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQVLEMSGMLILAMPGIVLATGFFL
LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
VTALILLLLCFLLFTVIEKLPGRNVKTD"
misc_feature complement(72917..74491)
/gene="thiP"
/locus_tag="b0067"
/gene_synonym="ECK0068; JW0066; sfuB; yabK"
/note="thiamine transporter membrane protein; Reviewed;
Region: thiP; PRK09433"
/db_xref="CDD:181853"
misc_feature complement(73757..74353)
/gene="thiP"
/locus_tag="b0067"
/gene_synonym="ECK0068; JW0066; sfuB; yabK"
/note="Transmembrane subunit (TM) found in Periplasmic
Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC)
transporters which generally bind type 2 PBPs. These types
of transporters consist of a PBP, two TMs, and two
cytoplasmic ABC ATPase subunits, and...; Region: TM_PBP2;
cd06261"
/db_xref="CDD:119394"
misc_feature complement(order(73757..73762,73769..73774,73781..73786,
73790..73795,73802..73807,73835..73840,73874..73879,
73886..73897,73916..73918,73925..73930,73970..73972,
74021..74023,74030..74035,74045..74047,74051..74056,
74063..74065,74069..74071,74078..74083,74186..74188,
74192..74197,74204..74233,74237..74248,74276..74278,
74291..74296,74303..74308))
/gene="thiP"
/locus_tag="b0067"
/gene_synonym="ECK0068; JW0066; sfuB; yabK"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:119394"
misc_feature complement(order(73880..73897,74186..74230))
/gene="thiP"
/locus_tag="b0067"
/gene_synonym="ECK0068; JW0066; sfuB; yabK"
/note="conserved gate region; other site"
/db_xref="CDD:119394"
misc_feature complement(order(73805..73807,73835..73837,73844..73846,
73877..73879,74096..74098,74186..74188))
/gene="thiP"
/locus_tag="b0067"
/gene_synonym="ECK0068; JW0066; sfuB; yabK"
/note="putative PBP binding loops; other site"
/db_xref="CDD:119394"
misc_feature complement(order(73949..73951,73961..73966,73982..74020))
/gene="thiP"
/locus_tag="b0067"
/gene_synonym="ECK0068; JW0066; sfuB; yabK"
/note="ABC-ATPase subunit interface; other site"
/db_xref="CDD:119394"
misc_feature complement(73004..73531)
/gene="thiP"
/locus_tag="b0067"
/gene_synonym="ECK0068; JW0066; sfuB; yabK"
/note="Transmembrane subunit (TM) found in Periplasmic
Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC)
transporters which generally bind type 2 PBPs. These types
of transporters consist of a PBP, two TMs, and two
cytoplasmic ABC ATPase subunits, and...; Region: TM_PBP2;
cd06261"
/db_xref="CDD:119394"
misc_feature complement(order(73004..73006,73034..73039,73076..73081,
73088..73099,73118..73120,73127..73132,73172..73174,
73223..73225,73232..73237,73247..73249,73253..73258,
73265..73267,73271..73273,73277..73282,73349..73351,
73355..73360,73367..73396,73400..73411,73451..73453,
73466..73471,73478..73483))
/gene="thiP"
/locus_tag="b0067"
/gene_synonym="ECK0068; JW0066; sfuB; yabK"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:119394"
misc_feature complement(order(73082..73099,73349..73393))
/gene="thiP"
/locus_tag="b0067"
/gene_synonym="ECK0068; JW0066; sfuB; yabK"
/note="conserved gate region; other site"
/db_xref="CDD:119394"
misc_feature complement(order(73004..73006,73034..73036,73043..73045,
73079..73081,73295..73297,73349..73351))
/gene="thiP"
/locus_tag="b0067"
/gene_synonym="ECK0068; JW0066; sfuB; yabK"
/note="putative PBP binding loops; other site"
/db_xref="CDD:119394"
misc_feature complement(order(73151..73153,73163..73168,73184..73222))
/gene="thiP"
/locus_tag="b0067"
/gene_synonym="ECK0068; JW0066; sfuB; yabK"
/note="ABC-ATPase subunit interface; other site"
/db_xref="CDD:119394"
gene complement(74497..75480)
/gene="thiB"
/locus_tag="b0068"
/gene_synonym="ECK0069; JW0067; sfuA; tbpA; yabL"
/db_xref="EcoGene:EG11574"
/db_xref="GeneID:946306"
CDS complement(74497..75480)
/gene="thiB"
/locus_tag="b0068"
/gene_synonym="ECK0069; JW0067; sfuA; tbpA; yabL"
/function="putative transport; Transport of small
molecules: Other"
/GO_component="GO:0042597 - periplasmic space"
/GO_process="GO:0009228 - thiamin biosynthetic process"
/note="thiamin-binding periplasmic protein;
periplasmic-binding component of ABC superfamily"
/codon_start=1
/transl_table=11
/product="thiamin transporter subunit"
/protein_id="NP_414610.1"
/db_xref="GI:16128062"
/db_xref="ASAP:ABE-0000249"
/db_xref="UniProtKB/Swiss-Prot:P31550"
/db_xref="EcoGene:EG11574"
/db_xref="GeneID:946306"
/translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
misc_feature complement(74500..75444)
/gene="thiB"
/locus_tag="b0068"
/gene_synonym="ECK0069; JW0067; sfuA; tbpA; yabL"
/note="thiamine transporter substrate binding subunit;
Provisional; Region: tbpA; PRK11205"
/db_xref="CDD:236883"
misc_feature complement(74500..75432)
/gene="thiB"
/locus_tag="b0068"
/gene_synonym="ECK0069; JW0067; sfuA; tbpA; yabL"
/note="ABC-type thiamine transport system, periplasmic
component [Coenzyme metabolism]; Region: TbpA; COG4143"
/db_xref="CDD:226624"
mat_peptide complement(74500..75426)
/gene="thiB"
/locus_tag="b0068"
/gene_synonym="ECK0069; JW0067; sfuA; tbpA; yabL"
/product="thiamin transporter subunit"
/experiment="N-terminus verified by Edman degradation:
PMID 12182833"
gene complement(75644..77299)
/gene="sgrR"
/locus_tag="b0069"
/gene_synonym="ECK0070; JW0068; yabN"
/db_xref="EcoGene:EG12094"
/db_xref="GeneID:944788"
CDS complement(75644..77299)
/gene="sgrR"
/locus_tag="b0069"
/gene_synonym="ECK0070; JW0068; yabN"
/function="transport; Not classified"
/GO_component="GO:0042597 - periplasmic space"
/note="putative transport protein"
/codon_start=1
/transl_table=11
/product="transcriptional DNA-binding transcriptional
activator of sgrS sRNA"
/protein_id="NP_414611.1"
/db_xref="GI:16128063"
/db_xref="ASAP:ABE-0000251"
/db_xref="UniProtKB/Swiss-Prot:P33595"
/db_xref="EcoGene:EG12094"
/db_xref="GeneID:944788"
/translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLKIKEIDYDQWHTGEIES
DIWLNSANFTLPLDFSVFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
SKAMVPLLHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
misc_feature complement(75647..77299)
/gene="sgrR"
/locus_tag="b0069"
/gene_synonym="ECK0070; JW0068; yabN"
/note="transcriptional regulator SgrR; Provisional;
Region: PRK13626"
/db_xref="CDD:184188"
misc_feature complement(76946..77287)
/gene="sgrR"
/locus_tag="b0069"
/gene_synonym="ECK0070; JW0068; yabN"
/note="Sugar transport-related sRNA regulator N-term;
Region: SgrR_N; pfam12793"
/db_xref="CDD:193269"
misc_feature complement(75659..76954)
/gene="sgrR"
/locus_tag="b0069"
/gene_synonym="ECK0070; JW0068; yabN"
/note="The C-terminal solute-binding domain of DNA-binding
transcriptional regulator SgrR is related to the ABC-type
oligopeptide-binding proteins and contains the type 2
periplasmic-binding fold; Region: PBP2_SgrR_like; cd08507"
/db_xref="CDD:173872"
gene 77367..77593
/gene="sgrS"
/locus_tag="b4577"
/gene_synonym="ECK0071; JWR0259; ryaA"
/db_xref="EcoGene:EG31149"
/db_xref="GeneID:4056038"
ncRNA 77367..77593
/gene="sgrS"
/locus_tag="b4577"
/gene_synonym="ECK0071; JWR0259; ryaA"
/ncRNA_class="antisense_RNA"
/product="sRNA antisense regulator destabilzes ptsG mRNA,
Hfq-dependent; regulated by sgrR"
/db_xref="ASAP:ABE-0285090"
/db_xref="EcoGene:EG31149"
/db_xref="GeneID:4056038"
gene 77388..77519
/gene="sgrT"
/locus_tag="b4662"
/gene_synonym="ECK4477"
/db_xref="EcoGene:EG14467"
/db_xref="GeneID:7751624"
CDS 77388..77519
/gene="sgrT"
/locus_tag="b4662"
/gene_synonym="ECK4477"
/codon_start=1
/transl_table=11
/product="Inhibitor of glucose uptake"
/protein_id="YP_002791237.1"
/db_xref="GI:226524697"
/db_xref="EcoGene:EG14467"
/db_xref="GeneID:7751624"
/translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
gene 77621..78799
/gene="setA"
/locus_tag="b0070"
/gene_synonym="ECK0072; JW0069; yabM"
/db_xref="EcoGene:EG11754"
/db_xref="GeneID:944793"
CDS 77621..78799
/gene="setA"
/locus_tag="b0070"
/gene_synonym="ECK0072; JW0069; yabM"
/function="putative transport; Not classified"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/GO_function="GO:0015543 - lactose/glucose efflux
transporter activity"
/GO_process="GO:0016052 - carbohydrate catabolic process"
/codon_start=1
/transl_table=11
/product="broad specificity sugar efflux system"
/protein_id="YP_025293.1"
/db_xref="GI:49175994"
/db_xref="ASAP:ABE-0000257"
/db_xref="UniProtKB/Swiss-Prot:P31675"
/db_xref="EcoGene:EG11754"
/db_xref="GeneID:944793"
/translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
RYGKRRMMVIAVAAGVLFYTGLIFFNSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
"
misc_feature 77666..78790
/gene="setA"
/locus_tag="b0070"
/gene_synonym="ECK0072; JW0069; yabM"
/note="The Major Facilitator Superfamily (MFS) is a large
and diverse group of secondary transporters that includes
uniporters, symporters, and antiporters. MFS proteins
facilitate the transport across cytoplasmic or internal
membranes of a variety of...; Region: MFS; cd06174"
/db_xref="CDD:119392"
misc_feature 77669..78793
/gene="setA"
/locus_tag="b0070"
/gene_synonym="ECK0072; JW0069; yabM"
/note="sugar efflux transporter; Region: 2A0120;
TIGR00899"
/db_xref="CDD:129977"
misc_feature order(77705..77707,77714..77722,77726..77731,77783..77785,
77792..77797,77804..77806,77816..77821,77825..77830,
77969..77974,77981..77986,77993..77998,78005..78007,
78041..78043,78050..78052,78059..78064,78080..78082,
78302..78304,78311..78316,78323..78328,78335..78337,
78377..78379,78389..78391,78401..78403,78410..78412,
78422..78424,78563..78565,78572..78577,78584..78586,
78596..78601,78608..78610,78638..78643,78650..78655,
78662..78667,78674..78676)
/gene="setA"
/locus_tag="b0070"
/gene_synonym="ECK0072; JW0069; yabM"
/note="putative substrate translocation pore; other site"
/db_xref="CDD:119392"
gene complement(78848..79453)
/gene="leuD"
/locus_tag="b0071"
/gene_synonym="ECK0073; JW0070"
/db_xref="EcoGene:EG11575"
/db_xref="GeneID:945642"
CDS complement(78848..79453)
/gene="leuD"
/locus_tag="b0071"
/gene_synonym="ECK0073; JW0070"
/EC_number="4.2.1.33"
/function="enzyme; Amino acid biosynthesis: Leucine"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0009098 - leucine biosynthetic process"
/note="isopropylmalate isomerase subunit"
/codon_start=1
/transl_table=11
/product="3-isopropylmalate dehydratase small subunit"
/protein_id="NP_414613.1"
/db_xref="GI:16128065"
/db_xref="ASAP:ABE-0000259"
/db_xref="UniProtKB/Swiss-Prot:P30126"
/db_xref="EcoGene:EG11575"
/db_xref="GeneID:945642"
/translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
mat_peptide complement(78851..79450)
/gene="leuD"
/locus_tag="b0071"
/gene_synonym="ECK0073; JW0070"
/product="3-isopropylmalate dehydratase small subunit"
/experiment="N-terminus verified by Edman degradation:
PMID 9600841"
misc_feature complement(78854..79447)
/gene="leuD"
/locus_tag="b0071"
/gene_synonym="ECK0073; JW0070"
/note="isopropylmalate isomerase small subunit;
Provisional; Region: leuD; PRK01641"
/db_xref="CDD:179314"
misc_feature complement(79010..79411)
/gene="leuD"
/locus_tag="b0071"
/gene_synonym="ECK0073; JW0070"
/note="Aconatase-like swivel domain of 3-isopropylmalate
dehydratase and related uncharacterized proteins.
3-isopropylmalate dehydratase catalyzes the isomerization
between 2-isopropylmalate and 3-isopropylmalate, via the
formation of 2-isopropylmaleate...; Region: IPMI_Swivel;
cd01577"
/db_xref="CDD:238809"
misc_feature complement(79196..79204)
/gene="leuD"
/locus_tag="b0071"
/gene_synonym="ECK0073; JW0070"
/note="substrate binding site [chemical binding]; other
site"
/db_xref="CDD:238809"
gene complement(79464..80864)
/gene="leuC"
/locus_tag="b0072"
/gene_synonym="ECK0074; JW0071"
/db_xref="EcoGene:EG11576"
/db_xref="GeneID:945076"
CDS complement(79464..80864)
/gene="leuC"
/locus_tag="b0072"
/gene_synonym="ECK0074; JW0071"
/EC_number="4.2.1.33"
/function="enzyme; Amino acid biosynthesis: Leucine"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0009098 - leucine biosynthetic process"
/note="3-isopropylmalate isomerase (dehydratase) subunit"
/codon_start=1
/transl_table=11
/product="3-isopropylmalate dehydratase large subunit"
/protein_id="NP_414614.1"
/db_xref="GI:16128066"
/db_xref="ASAP:ABE-0000261"
/db_xref="UniProtKB/Swiss-Prot:P0A6A6"
/db_xref="EcoGene:EG11576"
/db_xref="GeneID:945076"
/translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
AAAAVTGHFADIRNIK"
misc_feature complement(79470..80864)
/gene="leuC"
/locus_tag="b0072"
/gene_synonym="ECK0074; JW0071"
/note="3-isopropylmalate dehydratase large subunit;
Reviewed; Region: PRK00402"
/db_xref="CDD:234748"
mat_peptide complement(79467..80861)
/gene="leuC"
/locus_tag="b0072"
/gene_synonym="ECK0074; JW0071"
/product="3-isopropylmalate dehydratase large subunit"
/experiment="N-terminus verified by Edman degradation:
PMID 9298646,9600841,9740056"
misc_feature complement(79488..80780)
/gene="leuC"
/locus_tag="b0072"
/gene_synonym="ECK0074; JW0071"
/note="3-isopropylmalate dehydratase catalyzes the
isomerization between 2-isopropylmalate and
3-isopropylmalate; Region: IPMI; cd01583"
/db_xref="CDD:153133"
misc_feature complement(order(79560..79562,79575..79577,79632..79634,
80478..80483,80757..80759,80766..80768))
/gene="leuC"
/locus_tag="b0072"
/gene_synonym="ECK0074; JW0071"
/note="substrate binding site [chemical binding]; other
site"
/db_xref="CDD:153133"
misc_feature complement(order(79578..79580,79632..79637,79644..79646,
79824..79826,80475..80477))
/gene="leuC"
/locus_tag="b0072"
/gene_synonym="ECK0074; JW0071"
/note="ligand binding site [chemical binding]; other site"
/db_xref="CDD:153133"
gene complement(80867..81958)
/gene="leuB"
/locus_tag="b0073"
/gene_synonym="ECK0075; JW5807"
/db_xref="EcoGene:EG11577"
/db_xref="GeneID:944798"
CDS complement(80867..81958)
/gene="leuB"
/locus_tag="b0073"
/gene_synonym="ECK0075; JW5807"
/EC_number="1.1.1.85"
/function="enzyme; Amino acid biosynthesis: Leucine"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0009098 - leucine biosynthetic process"
/codon_start=1
/transl_table=11
/product="3-isopropylmalate dehydrogenase,
NAD(+)-dependent"
/protein_id="NP_414615.4"
/db_xref="GI:90111082"
/db_xref="ASAP:ABE-0000265"
/db_xref="UniProtKB/Swiss-Prot:P30125"
/db_xref="EcoGene:EG11577"
/db_xref="GeneID:944798"
/translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
RTGDLARGAAAVSTDEMGDIIARYVAEGV"
mat_peptide complement(80870..81955)
/gene="leuB"
/locus_tag="b0073"
/gene_synonym="ECK0075; JW5807"
/product="3-isopropylmalate dehydrogenase,
NAD(+)-dependent"
/experiment="N-terminus verified by Edman degradation:
PMID 9298646"
misc_feature complement(80873..81952)
/gene="leuB"
/locus_tag="b0073"
/gene_synonym="ECK0075; JW5807"
/note="3-isopropylmalate dehydrogenase; Provisional;
Region: PRK00772"
/db_xref="CDD:234832"
misc_feature complement(80891..81952)
/gene="leuB"
/locus_tag="b0073"
/gene_synonym="ECK0075; JW5807"
/note="tartrate dehydrogenase; Region: TTC; TIGR02089"
/db_xref="CDD:233718"
gene complement(81958..83529)
/gene="leuA"
/locus_tag="b0074"
/gene_synonym="ECK0076; JW0073"
/db_xref="EcoGene:EG11226"
/db_xref="GeneID:947465"
CDS complement(81958..83529)
/gene="leuA"
/locus_tag="b0074"
/gene_synonym="ECK0076; JW0073"
/EC_number="2.3.3.13"
/function="enzyme; Amino acid biosynthesis: Leucine"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0009098 - leucine biosynthetic process"
/codon_start=1
/transl_table=11
/product="2-isopropylmalate synthase"
/protein_id="NP_414616.1"
/db_xref="GI:16128068"
/db_xref="ASAP:ABE-0000267"
/db_xref="UniProtKB/Swiss-Prot:P09151"
/db_xref="EcoGene:EG11226"
/db_xref="GeneID:947465"
/translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITEYNVELVKYS
LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
LQRKAQHNENNKETV"
misc_feature complement(81994..83529)
/gene="leuA"
/locus_tag="b0074"
/gene_synonym="ECK0076; JW0073"
/note="2-isopropylmalate synthase; Validated; Region:
PRK00915"
/db_xref="CDD:234864"
mat_peptide complement(81961..83526)
/gene="leuA"
/locus_tag="b0074"
/gene_synonym="ECK0076; JW0073"
/product="2-isopropylmalate synthase"
/experiment="N-terminus verified by Edman degradation:
PMID 9298646"
misc_feature complement(82705..83511)
/gene="leuA"
/locus_tag="b0074"
/gene_synonym="ECK0076; JW0073"
/note="2-isopropylmalate synthase (IPMS), N-terminal
catalytic TIM barrel domain; Region: DRE_TIM_IPMS;
cd07940"
/db_xref="CDD:163678"
misc_feature complement(order(82918..82920,82924..82926,83017..83019,
83023..83025,83107..83109,83113..83115,83239..83241,
83305..83307,83398..83400,83479..83481,83488..83493))
/gene="leuA"
/locus_tag="b0074"
/gene_synonym="ECK0076; JW0073"
/note="active site"
/db_xref="CDD:163678"
misc_feature complement(order(83398..83400,83488..83493))
/gene="leuA"
/locus_tag="b0074"
/gene_synonym="ECK0076; JW0073"
/note="catalytic residues [active]"
/db_xref="CDD:163678"
misc_feature complement(order(82918..82920,82924..82926))
/gene="leuA"
/locus_tag="b0074"
/gene_synonym="ECK0076; JW0073"
/note="metal binding site [ion binding]; metal-binding
site"
/db_xref="CDD:163678"
misc_feature complement(82030..82425)
/gene="leuA"
/locus_tag="b0074"
/gene_synonym="ECK0076; JW0073"
/note="LeuA allosteric (dimerisation) domain; Region:
LeuA_dimer; pfam08502"
/db_xref="CDD:219870"
gene complement(83622..83708)
/gene="leuL"
/locus_tag="b0075"
/gene_synonym="ECK0077; JW0074; leuLP"
/db_xref="EcoGene:EG11280"
/db_xref="GeneID:946072"
CDS complement(83622..83708)
/gene="leuL"
/locus_tag="b0075"
/gene_synonym="ECK0077; JW0074; leuLP"
/function="leader; Amino acid biosynthesis: Leucine"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0009098 - leucine biosynthetic process"
/codon_start=1
/transl_table=11
/product="leu operon leader peptide"
/protein_id="NP_414617.1"
/db_xref="GI:16128069"
/db_xref="ASAP:ABE-0000269"
/db_xref="UniProtKB/Swiss-Prot:P0AD79"
/db_xref="EcoGene:EG11280"
/db_xref="GeneID:946072"
/translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
misc_feature complement(83625..83708)
/gene="leuL"
/locus_tag="b0075"
/gene_synonym="ECK0077; JW0074; leuLP"
/note="leu operon leader peptide; Provisional; Region:
PRK09925"
/db_xref="CDD:137592"
gene 84368..85312
/gene="leuO"
/locus_tag="b0076"
/gene_synonym="ECK0078; JW0075"
/db_xref="EcoGene:EG10531"
/db_xref="GeneID:949034"
CDS 84368..85312
/gene="leuO"
/locus_tag="b0076"
/gene_synonym="ECK0078; JW0075"
/function="putative regulator; Amino acid biosynthesis:
Leucine"
/GO_component="GO:0005737 - cytoplasm"
/GO_function="GO:0016563 - transcription activator
activity"
/GO_process="GO:0009098 - leucine biosynthetic process"
/GO_process="GO:0006350 - transcription"
/note="probable transcriptional activator for leuABCD
operon"
/codon_start=1
/transl_table=11
/product="DNA-binding transcriptional activator"
/protein_id="NP_414618.4"
/db_xref="GI:90111083"
/db_xref="ASAP:ABE-0000280"
/db_xref="UniProtKB/Swiss-Prot:P10151"
/db_xref="EcoGene:EG10531"
/db_xref="GeneID:949034"
/translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
misc_feature 84368..85309
/gene="leuO"
/locus_tag="b0076"
/gene_synonym="ECK0078; JW0075"
/note="leucine transcriptional activator; Reviewed;
Region: leuO; PRK09508"
/db_xref="CDD:181918"
misc_feature 84437..84613
/gene="leuO"
/locus_tag="b0076"
/gene_synonym="ECK0078; JW0075"
/note="Bacterial regulatory helix-turn-helix protein, lysR
family; Region: HTH_1; pfam00126"
/db_xref="CDD:215735"
misc_feature 84704..85300
/gene="leuO"
/locus_tag="b0076"
/gene_synonym="ECK0078; JW0075"
/note="The C-terminal substrate binding domain of
LysR-type transcriptional regulator LeuO, an activator of
leucine synthesis operon, contains the type 2 periplasmic
binding fold; Region: PBP2_LeuO; cd08466"
/db_xref="CDD:176155"
misc_feature order(84722..84724,84728..84733,84743..84745,84917..84919,
85025..85027,85226..85228)
/gene="leuO"
/locus_tag="b0076"
/gene_synonym="ECK0078; JW0075"
/note="putative substrate binding pocket [chemical
binding]; other site"
/db_xref="CDD:176155"
misc_feature order(84725..84727,84734..84739,84746..84751,84758..84763,
84767..84772,84779..84784,84788..84802,84806..84808,
84995..84997,85076..85090,85097..85102,85109..85114,
85121..85123,85163..85165)
/gene="leuO"
/locus_tag="b0076"
/gene_synonym="ECK0078; JW0075"
/note="putative dimerization interface [polypeptide
binding]; other site"
/db_xref="CDD:176155"
gene 85630..87354
/gene="ilvI"
/locus_tag="b0077"
/gene_synonym="ECK0079; JW0076"
/db_xref="EcoGene:EG10500"
/db_xref="GeneID:948793"
CDS 85630..87354
/gene="ilvI"
/locus_tag="b0077"
/gene_synonym="ECK0079; JW0076"
/EC_number="2.2.1.6"
/function="enzyme; Amino acid biosynthesis: Isoleucine,
Valine"
/experiment="N-terminus verified by Edman degradation:
PMID 3881131,9298646"
/note="acetolactate synthase III, valine sensitive, large
subunit"
/codon_start=1
/transl_table=11
/product="acetolactate synthase III, large subunit"
/protein_id="YP_025294.2"
/db_xref="GI:90111084"
/db_xref="ASAP:ABE-0000290"
/db_xref="UniProtKB/Swiss-Prot:P00893"
/db_xref="EcoGene:EG10500"
/db_xref="GeneID:948793"
/translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
TAGCHQQLKETVEALNLPVVCSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
WLSKTERT"
misc_feature 85630..87351
/gene="ilvI"
/locus_tag="b0077"
/gene_synonym="ECK0079; JW0076"
/note="acetolactate synthase 3 catalytic subunit;
Validated; Region: PRK07979"
/db_xref="CDD:181185"
misc_feature 85651..86118
/gene="ilvI"
/locus_tag="b0077"
/gene_synonym="ECK0079; JW0076"
/note="Pyrimidine (PYR) binding domain of POX and related
proteins; Region: TPP_PYR_POX_like; cd07035"
/db_xref="CDD:132918"
misc_feature order(85690..85692,85705..85710,85720..85722,85732..85734,
85759..85779,85786..85788,85795..85800,85807..85812,
85816..85824,85858..85860,85879..85881,85891..85893,
85900..85905)
/gene="ilvI"
/locus_tag="b0077"
/gene_synonym="ECK0079; JW0076"
/note="PYR/PP interface [polypeptide binding]; other site"
/db_xref="CDD:132918"
misc_feature order(85705..85713,85720..85722,85732..85734,85762..85764,
85768..85779,85783..85785,85858..85860,85867..85872,
85876..85881,85888..85890,85990..85992,85999..86001)
/gene="ilvI"
/locus_tag="b0077"
/gene_synonym="ECK0079; JW0076"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:132918"
misc_feature order(85705..85707,85780..85782,85858..85860,85870..85872)
/gene="ilvI"
/locus_tag="b0077"
/gene_synonym="ECK0079; JW0076"
/note="TPP binding site [chemical binding]; other site"
/db_xref="CDD:132918"
misc_feature 86215..86610
/gene="ilvI"
/locus_tag="b0077"
/gene_synonym="ECK0079; JW0076"
/note="Thiamine pyrophosphate enzyme, central domain;
Region: TPP_enzyme_M; pfam00205"
/db_xref="CDD:215786"
misc_feature 86743..87297
/gene="ilvI"
/locus_tag="b0077"
/gene_synonym="ECK0079; JW0076"
/note="Thiamine pyrophosphate (TPP) family,
Acetohydroxyacid synthase (AHAS) subfamily, TPP-binding
module; composed of proteins similar to the large
catalytic subunit of AHAS. AHAS catalyzes the condensation
of two molecules of pyruvate to give the...; Region:
TPP_AHAS; cd02015"
/db_xref="CDD:238973"
misc_feature order(86812..86823,86890..86892,86896..86898,86971..86979,
86986..86988,87052..87054)
/gene="ilvI"
/locus_tag="b0077"
/gene_synonym="ECK0079; JW0076"
/note="TPP-binding site [chemical binding]; other site"
/db_xref="CDD:238973"
misc_feature order(86881..86883,86890..86892,86896..86898,86983..86988,
86992..86997,87016..87018,87133..87138,87148..87150,
87157..87159)
/gene="ilvI"
/locus_tag="b0077"
/gene_synonym="ECK0079; JW0076"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:238973"
gene 87357..87848
/gene="ilvH"
/locus_tag="b0078"
/gene_synonym="brnP; ECK0080; JW0077"
/db_xref="EcoGene:EG10499"
/db_xref="GeneID:947267"
CDS 87357..87848
/gene="ilvH"
/locus_tag="b0078"
/gene_synonym="brnP; ECK0080; JW0077"
/EC_number="2.2.1.6"
/function="enzyme; Amino acid biosynthesis: Isoleucine,
Valine"
/note="acetolactate synthase III, valine sensitive, small
subunit"
/codon_start=1
/transl_table=11
/product="acetolactate synthase III, thiamin-dependent,
small subunit"
/protein_id="NP_414620.1"
/db_xref="GI:16128071"
/db_xref="ASAP:ABE-0000294"
/db_xref="UniProtKB/Swiss-Prot:P00894"
/db_xref="EcoGene:EG10499"
/db_xref="GeneID:947267"
/translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
RNTEIFRGQIIDVTPSLYTVQLAGTSGKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
IMR"
misc_feature 87357..87842
/gene="ilvH"
/locus_tag="b0078"
/gene_synonym="brnP; ECK0080; JW0077"
/note="acetolactate synthase 3 regulatory subunit;
Reviewed; Region: ilvH; PRK11895"
/db_xref="CDD:183365"
misc_feature 87363..87578
/gene="ilvH"
/locus_tag="b0078"
/gene_synonym="brnP; ECK0080; JW0077"
/note="N-terminal ACT domain of the Escherichia coli
IlvH-like regulatory subunit of acetohydroxyacid synthase
(AHAS); Region: ACT_AHAS; cd04878"
/db_xref="CDD:153150"
misc_feature order(87381..87383,87387..87389,87402..87404,87441..87446,
87459..87461,87486..87488)
/gene="ilvH"
/locus_tag="b0078"
/gene_synonym="brnP; ECK0080; JW0077"
/note="putative valine binding site [chemical binding];
other site"
/db_xref="CDD:153150"
misc_feature order(87393..87398,87405..87407,87426..87428,87435..87437,
87447..87461,87489..87491)
/gene="ilvH"
/locus_tag="b0078"
/gene_synonym="brnP; ECK0080; JW0077"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:153150"
misc_feature 87603..87830
/gene="ilvH"
/locus_tag="b0078"
/gene_synonym="brnP; ECK0080; JW0077"
/note="Small subunit of acetolactate synthase; Region:
ALS_ss_C; pfam10369"
/db_xref="CDD:204463"
gene 88028..89032
/gene="cra"
/locus_tag="b0080"
/gene_synonym="ECK0081; fruC; fruR; fruS?; JW0078; serR?;
shl"
/db_xref="EcoGene:EG10338"
/db_xref="GeneID:944804"
CDS 88028..89032
/gene="cra"
/locus_tag="b0080"
/gene_synonym="ECK0081; fruC; fruR; fruS?; JW0078; serR?;
shl"
/function="regulator; Energy metabolism, carbon:
Glycolysis"
/GO_component="GO:0005737 - cytoplasm"
/GO_function="GO:0016563 - transcription activator
activity"
/GO_function="GO:0016564 - transcription repressor
activity"
/GO_process="GO:0006096 - glycolysis"
/GO_process="GO:0006350 - transcription"
/note="transcriptional repressor of fru operon and others"
/codon_start=1
/transl_table=11
/product="DNA-binding transcriptional repressor-activator
for carbon metabolism"
/protein_id="NP_414622.1"
/db_xref="GI:16128073"
/db_xref="ASAP:ABE-0000299"
/db_xref="UniProtKB/Swiss-Prot:P0ACP1"
/db_xref="EcoGene:EG10338"
/db_xref="GeneID:944804"
/translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
"
misc_feature 88028..89011
/gene="cra"
/locus_tag="b0080"
/gene_synonym="ECK0081; fruC; fruR; fruS?; JW0078; serR?;
shl"
/note="DNA-binding transcriptional regulator FruR;
Provisional; Region: PRK11303"
/db_xref="CDD:236897"
misc_feature 88040..88201
/gene="cra"
/locus_tag="b0080"
/gene_synonym="ECK0081; fruC; fruR; fruS?; JW0078; serR?;
shl"
/note="Helix-turn-helix (HTH) DNA binding domain of the
LacI family of transcriptional regulators; Region:
HTH_LacI; cd01392"
/db_xref="CDD:143331"
misc_feature order(88061..88075,88079..88084,88091..88093,88115..88120,
88127..88129,88166..88168,88175..88177,88184..88189,
88193..88198)
/gene="cra"
/locus_tag="b0080"
/gene_synonym="ECK0081; fruC; fruR; fruS?; JW0078; serR?;
shl"
/note="DNA binding site [nucleotide binding]"
/db_xref="CDD:143331"
misc_feature 88166..88195
/gene="cra"
/locus_tag="b0080"
/gene_synonym="ECK0081; fruC; fruR; fruS?; JW0078; serR?;
shl"
/note="domain linker motif; other site"
/db_xref="CDD:143331"
misc_feature 88214..89005
/gene="cra"
/locus_tag="b0080"
/gene_synonym="ECK0081; fruC; fruR; fruS?; JW0078; serR?;
shl"
/note="Ligand binding domain of DNA transcription
repressor specific for fructose (FruR) and its close
homologs; Region: PBP1_FruR; cd06274"
/db_xref="CDD:107269"
misc_feature order(88238..88240,88268..88273,88277..88282,88289..88291,
88304..88306,88313..88315,88319..88321,88373..88375,
88778..88780,88868..88870,88874..88876)
/gene="cra"
/locus_tag="b0080"
/gene_synonym="ECK0081; fruC; fruR; fruS?; JW0078; serR?;
shl"
/note="dimerization interface [polypeptide binding]; other
site"
/db_xref="CDD:107269"
misc_feature order(88250..88252,88262..88264,88463..88468,88499..88510,
88616..88618,88763..88765,88847..88849)
/gene="cra"
/locus_tag="b0080"
/gene_synonym="ECK0081; fruC; fruR; fruS?; JW0078; serR?;
shl"
/note="ligand binding site [chemical binding]; other site"
/db_xref="CDD:107269"
gene 89634..90092
/gene="mraZ"
/locus_tag="b0081"
/gene_synonym="ECK0082; JW0079; yabB"
/db_xref="EcoGene:EG11084"
/db_xref="GeneID:944810"
CDS 89634..90092
/gene="mraZ"
/locus_tag="b0081"
/gene_synonym="ECK0082; JW0079; yabB"
/codon_start=1
/transl_table=11
/product="RsmH methytransferase inhibitor"
/protein_id="NP_414623.1"
/db_xref="GI:16128074"
/db_xref="ASAP:ABE-0000302"
/db_xref="UniProtKB/Swiss-Prot:P22186"
/db_xref="EcoGene:EG11084"
/db_xref="GeneID:944810"
/translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIYHPC
LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
misc_feature 89634..90086
/gene="mraZ"
/locus_tag="b0081"
/gene_synonym="ECK0082; JW0079; yabB"
/note="mraZ protein; Region: TIGR00242"
/db_xref="CDD:129345"
misc_feature 89634..89861
/gene="mraZ"
/locus_tag="b0081"
/gene_synonym="ECK0082; JW0079; yabB"
/note="MraZ protein; Region: MraZ; pfam02381"
/db_xref="CDD:111290"
misc_feature 89862..90038
/gene="mraZ"
/locus_tag="b0081"
/gene_synonym="ECK0082; JW0079; yabB"
/note="MraZ protein; Region: MraZ; pfam02381"
/db_xref="CDD:111290"
gene 90094..91035
/gene="rsmH"
/locus_tag="b0082"
/gene_synonym="ECK0083; JW0080; mraW; yabC"
/db_xref="EcoGene:EG11085"
/db_xref="GeneID:944806"
CDS 90094..91035
/gene="rsmH"
/locus_tag="b0082"
/gene_synonym="ECK0083; JW0080; mraW; yabC"
/EC_number="2.1.1.-"
/function="enzyme; rRNA modification"
/experiment="N-terminus verified by Edman degradation:
PMID 10572301"
/GO_process="GO:0042158 - lipoprotein biosynthetic
process"
/note="putative apolipoprotein"
/codon_start=1
/transl_table=11
/product="16S rRNA m(4)C1402 methyltransferase,
SAM-dependent"
/protein_id="NP_414624.1"
/db_xref="GI:16128075"
/db_xref="ASAP:ABE-0000304"
/db_xref="UniProtKB/Swiss-Prot:P60390"
/db_xref="EcoGene:EG11085"
/db_xref="GeneID:944806"
/translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
misc_feature 90103..91026
/gene="rsmH"
/locus_tag="b0082"
/gene_synonym="ECK0083; JW0080; mraW; yabC"
/note="16S rRNA (cytosine(1402)-N(4))-methyltransferase;
Region: TIGR00006"
/db_xref="CDD:232778"
misc_feature 90106..91029
/gene="rsmH"
/locus_tag="b0082"
/gene_synonym="ECK0083; JW0080; mraW; yabC"
/note="16S rRNA m(4)C1402 methyltranserfase; Provisional;
Region: PRK00050"
/db_xref="CDD:234597"
gene 91032..91397
/gene="ftsL"
/locus_tag="b0083"
/gene_synonym="ECK0084; JW0081; mraR; yabD"
/db_xref="EcoGene:EG11086"
/db_xref="GeneID:944803"
CDS 91032..91397
/gene="ftsL"
/locus_tag="b0083"
/gene_synonym="ECK0084; JW0081; mraR; yabD"
/function="phenotype; Cell division"
/note="cell division protein; ingrowth of wall at septum"
/codon_start=1
/transl_table=11
/product="membrane bound cell division protein at septum
containing leucine zipper motif"
/protein_id="NP_414625.1"
/db_xref="GI:16128076"
/db_xref="ASAP:ABE-0000306"
/db_xref="UniProtKB/Swiss-Prot:P0AEN4"
/db_xref="EcoGene:EG11086"
/db_xref="GeneID:944803"
/translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
EKLQMQHVDPSQENIVVQK"
misc_feature 91080..91394
/gene="ftsL"
/locus_tag="b0083"
/gene_synonym="ECK0084; JW0081; mraR; yabD"
/note="cell division protein FtsL; Provisional; Region:
PRK10772"
/db_xref="CDD:182717"
gene 91413..93179
/gene="ftsI"
/locus_tag="b0084"
/gene_synonym="ECK0085; JW0082; pbpB; sep"
/db_xref="EcoGene:EG10341"
/db_xref="GeneID:944799"
CDS 91413..93179
/gene="ftsI"
/locus_tag="b0084"
/gene_synonym="ECK0085; JW0082; pbpB; sep"
/function="enzyme; Cell division"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/GO_process="GO:0009252 - peptidoglycan biosynthetic
process"
/GO_process="GO:0042493 - response to drug"
/note="septum formation; penicillin-binding protein 3;
peptidoglycan synthetase"
/codon_start=1
/transl_table=11
/product="transpeptidase involved in septal peptidoglycan
synthesis (penicillin-binding protein 3)"
/protein_id="NP_414626.1"
/db_xref="GI:16128077"
/db_xref="ASAP:ABE-0000309"
/db_xref="UniProtKB/Swiss-Prot:P0AD68"
/db_xref="EcoGene:EG10341"
/db_xref="GeneID:944799"
/translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
ALTTGDKNEFVINQGEGTGGRS"
misc_feature 91416..93149
/gene="ftsI"
/locus_tag="b0084"
/gene_synonym="ECK0085; JW0082; pbpB; sep"
/note="peptidoglycan synthase FtsI; Provisional; Region:
PRK15105"
/db_xref="CDD:185060"
misc_feature 91623..92078
/gene="ftsI"
/locus_tag="b0084"
/gene_synonym="ECK0085; JW0082; pbpB; sep"
/note="Penicillin-binding Protein dimerisation domain;
Region: PBP_dimer; pfam03717"
/db_xref="CDD:217689"
misc_feature 92190..93074
/gene="ftsI"
/locus_tag="b0084"
/gene_synonym="ECK0085; JW0082; pbpB; sep"
/note="Penicillin binding protein transpeptidase domain;
Region: Transpeptidase; pfam00905"
/db_xref="CDD:216183"
gene 93166..94653
/gene="murE"
/locus_tag="b0085"
/gene_synonym="ECK0086; JW0083"
/db_xref="EcoGene:EG10621"
/db_xref="GeneID:944791"
CDS 93166..94653
/gene="murE"
/locus_tag="b0085"
/gene_synonym="ECK0086; JW0083"
/EC_number="6.3.2.13"
/function="enzyme; Murein sacculus, peptidoglycan"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_process="GO:0009252 - peptidoglycan biosynthetic
process"
/codon_start=1
/transl_table=11
/product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:meso-
diaminopimelate ligase"
/protein_id="NP_414627.1"
/db_xref="GI:16128078"
/db_xref="ASAP:ABE-0000311"
/db_xref="UniProtKB/Swiss-Prot:P22188"
/db_xref="EcoGene:EG10621"
/db_xref="GeneID:944791"
/translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGEISAVMGTVGNGLLGKVIPTENTTG
SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
misc_feature 93166..94647
/gene="murE"
/locus_tag="b0085"
/gene_synonym="ECK0086; JW0083"
/note="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
6-diaminopimelate ligase; Provisional; Region: murE;
PRK00139"
/db_xref="CDD:234660"
mat_peptide 93169..94650
/gene="murE"
/locus_tag="b0085"
/gene_synonym="ECK0086; JW0083"
/product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:meso-
diaminopimelate ligase"
/experiment="N-terminus verified by Edman degradation:
PMID 2198024,2269304"
misc_feature 93238..93471
/gene="murE"
/locus_tag="b0085"
/gene_synonym="ECK0086; JW0083"
/note="Mur ligase family, catalytic domain; Region:
Mur_ligase; pfam01225"
/db_xref="CDD:216373"
misc_feature 93538..94092
/gene="murE"
/locus_tag="b0085"
/gene_synonym="ECK0086; JW0083"
/note="Mur ligase middle domain; Region: Mur_ligase_M;
pfam08245"
/db_xref="CDD:219763"
misc_feature 94180..94440
/gene="murE"
/locus_tag="b0085"
/gene_synonym="ECK0086; JW0083"
/note="Mur ligase family, glutamate ligase domain; Region:
Mur_ligase_C; pfam02875"
/db_xref="CDD:217262"
gene 94650..96008
/gene="murF"
/locus_tag="b0086"
/gene_synonym="ECK0087; JW0084; mra"
/db_xref="EcoGene:EG10622"
/db_xref="GeneID:944813"
CDS 94650..96008
/gene="murF"
/locus_tag="b0086"
/gene_synonym="ECK0087; JW0084; mra"
/EC_number="6.3.2.10"
/function="enzyme; Murein sacculus, peptidoglycan"
/function="1.6.7 metabolism; macromolecules (cellular
constituent) biosynthesis; peptidoglycan (murein)"
/function="1.7.34 metabolism; central intermediary
metabolism; peptidoglycan (murein)"
/function="1.7.34 metabolism; central intermediary
metabolism; peptidoglycan (murein) turnover, recycling"
/function="7.1 location of gene products; cytoplasm"
/experiment="N-terminus verified by Edman degradation:
PMID 2186811"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0009252 - peptidoglycan biosynthetic
process"
/GO_process="GO:0000270 - peptidoglycan metabolic process"
/codon_start=1
/transl_table=11
/product="UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-
alanine ligase"
/protein_id="NP_414628.1"
/db_xref="GI:16128079"
/db_xref="ASAP:ABE-0000313"
/db_xref="UniProtKB/Swiss-Prot:P11880"
/db_xref="EcoGene:EG10622"
/db_xref="GeneID:944813"
/translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
QSHAISTASGVGEHFADKTALITRLKLLIAEQQVITILVKGSRSAAMEEVVRALQENG
TC"
misc_feature 94650..96005
/gene="murF"
/locus_tag="b0086"
/gene_synonym="ECK0087; JW0084; mra"
/note="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine
ligase; Reviewed; Region: murF; PRK10773"
/db_xref="CDD:182718"
misc_feature 94728..94934
/gene="murF"
/locus_tag="b0086"
/gene_synonym="ECK0087; JW0084; mra"
/note="Mur ligase family, catalytic domain; Region:
Mur_ligase; pfam01225"
/db_xref="CDD:216373"
misc_feature 94965..95498
/gene="murF"
/locus_tag="b0086"
/gene_synonym="ECK0087; JW0084; mra"
/note="Mur ligase middle domain; Region: Mur_ligase_M;
pfam08245"
/db_xref="CDD:219763"
misc_feature 95586..95858
/gene="murF"
/locus_tag="b0086"
/gene_synonym="ECK0087; JW0084; mra"
/note="Mur ligase family, glutamate ligase domain; Region:
Mur_ligase_C; pfam02875"
/db_xref="CDD:217262"
gene 96002..97084
/gene="mraY"
/locus_tag="b0087"
/gene_synonym="ECK0088; JW0085; murX"
/db_xref="EcoGene:EG10604"
/db_xref="GeneID:944814"
CDS 96002..97084
/gene="mraY"
/locus_tag="b0087"
/gene_synonym="ECK0088; JW0085; murX"
/EC_number="2.7.8.13"
/function="enzyme; Murein sacculus, peptidoglycan"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/GO_process="GO:0009252 - peptidoglycan biosynthetic
process"
/note="phospho-N-acetylmuramoyl-pentapeptide transferase?"
/codon_start=1
/transl_table=11
/product="phospho-N-acetylmuramoyl-pentapeptide
transferase"
/protein_id="NP_414629.1"
/db_xref="GI:16128080"
/db_xref="ASAP:ABE-0000316"
/db_xref="UniProtKB/Swiss-Prot:P0A6W3"
/db_xref="EcoGene:EG10604"
/db_xref="GeneID:944814"
/translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
EPRVIVRFWIISLMLVLIGLATLKVR"
misc_feature 96047..97081
/gene="mraY"
/locus_tag="b0087"
/gene_synonym="ECK0088; JW0085; murX"
/note="phospho-N-acetylmuramoyl-pentapeptide-transferase;
Provisional; Region: mraY; PRK00108"
/db_xref="CDD:234638"
misc_feature 96182..97069
/gene="mraY"
/locus_tag="b0087"
/gene_synonym="ECK0088; JW0085; murX"
/note="Phospho-N-acetylmuramoyl-pentapeptide-transferase
(mraY) is an enzyme responsible for the formation of the
first lipid intermediate in the synthesis of bacterial
cell wall peptidoglycan. It catalyzes the formation of...;
Region: GT_MraY; cd06852"
/db_xref="CDD:133462"
misc_feature 96344..96349
/gene="mraY"
/locus_tag="b0087"
/gene_synonym="ECK0088; JW0085; murX"
/note="Mg++ binding site [ion binding]; other site"
/db_xref="CDD:133462"
misc_feature 96791..96802
/gene="mraY"
/locus_tag="b0087"
/gene_synonym="ECK0088; JW0085; murX"
/note="putative catalytic motif [active]"
/db_xref="CDD:133462"
misc_feature order(96950..96952,96968..96982)
/gene="mraY"
/locus_tag="b0087"
/gene_synonym="ECK0088; JW0085; murX"
/note="putative substrate binding site [chemical binding];
other site"
/db_xref="CDD:133462"
gene 97087..98403
/gene="murD"
/locus_tag="b0088"
/gene_synonym="ECK0089; JW0086"
/db_xref="EcoGene:EG10620"
/db_xref="GeneID:944818"
CDS 97087..98403
/gene="murD"
/locus_tag="b0088"
/gene_synonym="ECK0089; JW0086"
/EC_number="6.3.2.9"
/function="enzyme; Murein sacculus, peptidoglycan"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0009252 - peptidoglycan biosynthetic
process"
/codon_start=1
/transl_table=11
/product="UDP-N-acetylmuramoyl-L-alanine:D-glutamate
ligase"
/protein_id="NP_414630.1"
/db_xref="GI:16128081"
/db_xref="ASAP:ABE-0000318"
/db_xref="UniProtKB/Swiss-Prot:P14900"
/db_xref="EcoGene:EG10620"
/db_xref="GeneID:944818"
/translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
misc_feature 97087..98400
/gene="murD"
/locus_tag="b0088"
/gene_synonym="ECK0089; JW0086"
/note="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
synthetase; Provisional; Region: murD; PRK03806"
/db_xref="CDD:179651"
mat_peptide 97090..98400
/gene="murD"
/locus_tag="b0088"
/gene_synonym="ECK0089; JW0086"
/product="UDP-N-acetylmuramoyl-L-alanine:D-glutamate
ligase"
/experiment="N-terminus verified by Edman degradation:
PMID 1765076"
misc_feature 97417..97896
/gene="murD"
/locus_tag="b0088"
/gene_synonym="ECK0089; JW0086"
/note="Mur ligase middle domain; Region: Mur_ligase_M;
pfam08245"
/db_xref="CDD:219763"
misc_feature 97987..>98082
/gene="murD"
/locus_tag="b0088"
/gene_synonym="ECK0089; JW0086"
/note="Mur ligase family, glutamate ligase domain; Region:
Mur_ligase_C; pfam02875"
/db_xref="CDD:217262"
gene 98403..99647
/gene="ftsW"
/locus_tag="b0089"
/gene_synonym="ECK0090; JW0087"
/db_xref="EcoGene:EG10344"
/db_xref="GeneID:946322"
CDS 98403..99647
/gene="ftsW"
/locus_tag="b0089"
/gene_synonym="ECK0090; JW0087"
/function="membrane; Cell division"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/note="cell division; membrane protein involved in shape
determination"
/codon_start=1
/transl_table=11
/product="lipid II flippase; integral membrane protein
involved in stabilizing FstZ ring during cell division"
/protein_id="NP_414631.1"
/db_xref="GI:16128082"
/db_xref="ASAP:ABE-0000320"
/db_xref="UniProtKB/Swiss-Prot:P0ABG4"
/db_xref="EcoGene:EG10344"
/db_xref="GeneID:946322"
/translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
MLLRIDYETRLEKAQAFVRGSR"
misc_feature 98433..99644
/gene="ftsW"
/locus_tag="b0089"
/gene_synonym="ECK0090; JW0087"
/note="cell division protein FtsW; Provisional; Region:
PRK10774"
/db_xref="CDD:182719"
gene 99644..100711
/gene="murG"
/locus_tag="b0090"
/gene_synonym="ECK0091; JW0088"
/db_xref="EcoGene:EG10623"
/db_xref="GeneID:946321"
CDS 99644..100711
/gene="murG"
/locus_tag="b0090"
/gene_synonym="ECK0091; JW0088"
/EC_number="2.4.1.227"
/function="enzyme; Murein sacculus, peptidoglycan"
/function="1.6.7 metabolism; macromolecules (cellular
constituent) biosynthesis; peptidoglycan (murein)"
/function="7.3 location of gene products; inner membrane"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/GO_process="GO:0009252 - peptidoglycan biosynthetic
process"
/codon_start=1
/transl_table=11
/product="N-acetylglucosaminyl transferase"
/protein_id="NP_414632.1"
/db_xref="GI:16128083"
/db_xref="ASAP:ABE-0000322"
/db_xref="UniProtKB/Swiss-Prot:P17443"
/db_xref="EcoGene:EG10623"
/db_xref="GeneID:946321"
/translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
AASIPDATERVANEVSRVARA"
mat_peptide 99647..100708
/gene="murG"
/locus_tag="b0090"
/gene_synonym="ECK0091; JW0088"
/product="N-acetylglucosaminyl transferase"
/experiment="N-terminus verified by Edman degradation:
PMID 1649817"
misc_feature 99659..100708
/gene="murG"
/locus_tag="b0090"
/gene_synonym="ECK0091; JW0088"
/note="undecaprenyldiphospho-muramoylpentapeptide beta-N-
acetylglucosaminyltransferase; Provisional; Region: murG;
PRK00726"
/db_xref="CDD:234825"
misc_feature 99662..100687
/gene="murG"
/locus_tag="b0090"
/gene_synonym="ECK0091; JW0088"
/note="MurG is an N-acetylglucosaminyltransferase, the
last enzyme involved in the intracellular phase of
peptidoglycan biosynthesis. It transfers
N-acetyl-D-glucosamine (GlcNAc) from UDP-GlcNAc to the C4
hydroxyl of a lipid-linked N-acetylmuramoyl
pentapeptide...; Region: GT1_MurG; cd03785"
/db_xref="CDD:99961"
misc_feature order(99692..99694,100022..100024,100214..100216,
100292..100294,100370..100372,100382..100384,
100427..100438,100445..100447,100484..100486,
100502..100507,100514..100516)
/gene="murG"
/locus_tag="b0090"
/gene_synonym="ECK0091; JW0088"
/note="active site"
/db_xref="CDD:99961"
misc_feature order(99863..99865,99872..99874,99905..99907,99989..99991,
100064..100066)
/gene="murG"
/locus_tag="b0090"
/gene_synonym="ECK0091; JW0088"
/note="homodimer interface [polypeptide binding]; other
site"
/db_xref="CDD:99961"
gene 100765..102240
/gene="murC"
/locus_tag="b0091"
/gene_synonym="ECK0092; JW0089"
/db_xref="EcoGene:EG10619"
/db_xref="GeneID:946153"
CDS 100765..102240
/gene="murC"
/locus_tag="b0091"
/gene_synonym="ECK0092; JW0089"
/EC_number="6.3.2.8"
/function="enzyme; Murein sacculus, peptidoglycan"
/experiment="N-terminus verified by Edman degradation:
PMID 7601127"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_process="GO:0009252 - peptidoglycan biosynthetic
process"
/codon_start=1
/transl_table=11
/product="UDP-N-acetylmuramate:L-alanine ligase"
/protein_id="NP_414633.1"
/db_xref="GI:16128084"
/db_xref="ASAP:ABE-0000324"
/db_xref="UniProtKB/Swiss-Prot:P17952"
/db_xref="EcoGene:EG10619"
/db_xref="GeneID:946153"
/translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPARVAEML
APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
misc_feature 100801..102216
/gene="murC"
/locus_tag="b0091"
/gene_synonym="ECK0092; JW0089"
/note="UDP-N-acetylmuramate--L-alanine ligase;
Provisional; Region: murC; PRK00421"
/db_xref="CDD:234757"
misc_feature 100825..101124
/gene="murC"
/locus_tag="b0091"
/gene_synonym="ECK0092; JW0089"
/note="Mur ligase family, catalytic domain; Region:
Mur_ligase; pfam01225"
/db_xref="CDD:216373"
misc_feature 101140..101640
/gene="murC"
/locus_tag="b0091"
/gene_synonym="ECK0092; JW0089"
/note="Mur ligase middle domain; Region: Mur_ligase_M;
pfam08245"
/db_xref="CDD:219763"
misc_feature 101770..102024
/gene="murC"
/locus_tag="b0091"
/gene_synonym="ECK0092; JW0089"
/note="Mur ligase family, glutamate ligase domain; Region:
Mur_ligase_C; pfam02875"
/db_xref="CDD:217262"
gene 102233..103153
/gene="ddlB"
/locus_tag="b0092"
/gene_synonym="ddl; ECK0093; JW0090"
/db_xref="EcoGene:EG10214"
/db_xref="GeneID:946324"
CDS 102233..103153
/gene="ddlB"
/locus_tag="b0092"
/gene_synonym="ddl; ECK0093; JW0090"
/EC_number="6.3.2.4"
/function="enzyme; Murein sacculus, peptidoglycan"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0009252 - peptidoglycan biosynthetic
process"
/note="D-alanine-D-alanine ligase B, affects cell
division"
/codon_start=1
/transl_table=11
/product="D-alanine:D-alanine ligase"
/protein_id="NP_414634.1"
/db_xref="GI:16128085"
/db_xref="ASAP:ABE-0000326"
/db_xref="UniProtKB/Swiss-Prot:P07862"
/db_xref="EcoGene:EG10214"
/db_xref="GeneID:946324"
/translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
MTSHSLVPMAARQAGMSFSQLVVRILELAD"
misc_feature 102233..103150
/gene="ddlB"
/locus_tag="b0092"
/gene_synonym="ddl; ECK0093; JW0090"
/note="D-alanine--D-alanine ligase; Reviewed; Region: ddl;
PRK01372"
/db_xref="CDD:234948"
mat_peptide 102236..103150
/gene="ddlB"
/locus_tag="b0092"
/gene_synonym="ddl; ECK0093; JW0090"
/product="D-alanine:D-alanine ligase"
/experiment="N-terminus verified by Edman degradation:
PMID 1554356"
misc_feature 102242..102490
/gene="ddlB"
/locus_tag="b0092"
/gene_synonym="ddl; ECK0093; JW0090"
/note="D-ala D-ala ligase N-terminus; Region:
Dala_Dala_lig_N; pfam01820"
/db_xref="CDD:216721"
misc_feature 102539..103141
/gene="ddlB"
/locus_tag="b0092"
/gene_synonym="ddl; ECK0093; JW0090"
/note="D-ala D-ala ligase C-terminus; Region:
Dala_Dala_lig_C; pfam07478"
/db_xref="CDD:203643"
gene 103155..103985
/gene="ftsQ"
/locus_tag="b0093"
/gene_synonym="ECK0094; JW0091"
/db_xref="EcoGene:EG10342"
/db_xref="GeneID:944823"
CDS 103155..103985
/gene="ftsQ"
/locus_tag="b0093"
/gene_synonym="ECK0094; JW0091"
/function="phenotype; Cell division"
/GO_component="GO:0019866 - organelle inner membrane"
/note="cell division protein; ingrowth of wall at septum"
/codon_start=1
/transl_table=11
/product="divisome assembly protein, membrane anchored
protein involved in growth of wall at septum"
/protein_id="NP_414635.1"
/db_xref="GI:16128086"
/db_xref="ASAP:ABE-0000329"
/db_xref="UniProtKB/Swiss-Prot:P06136"
/db_xref="EcoGene:EG10342"
/db_xref="GeneID:944823"
/translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPERTSKQVLPML
YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
"
misc_feature 103155..103946
/gene="ftsQ"
/locus_tag="b0093"
/gene_synonym="ECK0094; JW0091"
/note="cell division protein FtsQ; Provisional; Region:
PRK10775"
/db_xref="CDD:182720"
misc_feature 103323..103532
/gene="ftsQ"
/locus_tag="b0093"
/gene_synonym="ECK0094; JW0091"
/note="POTRA domain, FtsQ-type; Region: POTRA_1;
pfam08478"
/db_xref="CDD:219857"
misc_feature 103539..103895
/gene="ftsQ"
/locus_tag="b0093"
/gene_synonym="ECK0094; JW0091"
/note="Cell division protein FtsQ; Region: FtsQ;
pfam03799"
/db_xref="CDD:217736"
gene 103982..105244
/gene="ftsA"
/locus_tag="b0094"
/gene_synonym="divA; ECK0095; JW0092"
/db_xref="EcoGene:EG10339"
/db_xref="GeneID:944778"
CDS 103982..105244
/gene="ftsA"
/locus_tag="b0094"
/gene_synonym="divA; ECK0095; JW0092"
/function="phenotype; Cell division"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/note="ATP-binding cell division protein, septation
process, complexes with FtsZ, associated with junctions of
inner and outer membranes"
/codon_start=1
/transl_table=11
/product="ATP-binding cell division protein involved in
recruitment of FtsK to Z ring"
/protein_id="NP_414636.1"
/db_xref="GI:16128087"
/db_xref="ASAP:ABE-0000331"
/db_xref="UniProtKB/Swiss-Prot:P0ABH0"
/db_xref="EcoGene:EG10339"
/db_xref="GeneID:944778"
/translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
GEAEVEKRVTASVGSWIKRLNSWLRKEF"
misc_feature 103982..105241
/gene="ftsA"
/locus_tag="b0094"
/gene_synonym="divA; ECK0095; JW0092"
/note="cell division protein FtsA; Reviewed; Region: ftsA;
PRK09472"
/db_xref="CDD:181887"
misc_feature 104282..>104665
/gene="ftsA"
/locus_tag="b0094"
/gene_synonym="divA; ECK0095; JW0092"
/note="Nucleotide-Binding Domain of the sugar
kinase/HSP70/actin superfamily; Region:
NBD_sugar-kinase_HSP70_actin; cd00012"
/db_xref="CDD:212657"
misc_feature 104597..105058
/gene="ftsA"
/locus_tag="b0094"
/gene_synonym="divA; ECK0095; JW0092"
/note="Cell division protein FtsA; Region: FtsA;
pfam14450"
/db_xref="CDD:222760"
STS 104533..104769
/gene="ftsA"
/locus_tag="b0094"
/gene_synonym="divA; ECK0095; JW0092"
/standard_name="G34047"
/db_xref="UniSTS:10389"
gene 105305..106456
/gene="ftsZ"
/locus_tag="b0095"
/gene_synonym="ECK0096; JW0093; sfiB; sulB"
/db_xref="EcoGene:EG10347"
/db_xref="GeneID:944786"
CDS 105305..106456
/gene="ftsZ"
/locus_tag="b0095"
/gene_synonym="ECK0096; JW0093; sfiB; sulB"
/function="enzyme; Cell division"
/experiment="N-terminus verified by Edman degradation:
PMID 9298646"
/GO_component="GO:0005737 - cytoplasm"
/note="cell division; forms circumferential ring;
tubulin-like GTP-binding protein and GTPase"
/codon_start=1
/transl_table=11
/product="GTP-binding tubulin-like cell division protein"
/protein_id="NP_414637.1"
/db_xref="GI:16128088"
/db_xref="ASAP:ABE-0000333"
/db_xref="UniProtKB/Swiss-Prot:P0A9A6"
/db_xref="EcoGene:EG10347"
/db_xref="GeneID:944786"
/translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
misc_feature 105305..106453
/gene="ftsZ"
/locus_tag="b0095"
/gene_synonym="ECK0096; JW0093; sfiB; sulB"
/note="cell division protein FtsZ; Validated; Region:
PRK09330"
/db_xref="CDD:236468"
misc_feature 105374..106246
/gene="ftsZ"
/locus_tag="b0095"
/gene_synonym="ECK0096; JW0093; sfiB; sulB"
/note="FtsZ is a GTPase that is similar to the eukaryotic
tubulins and is essential for cell division in
prokaryotes. FtsZ is capable of polymerizing in a
GTP-driven process into structures similar to those formed
by tubulin. FtsZ forms a ring-shaped septum at...; Region:
FtsZ_type1; cd02201"
/db_xref="CDD:100021"
misc_feature order(105374..105376,105446..105448,105611..105631,
105704..105709,105728..105730,105797..105799,
105848..105850,105857..105862,105869..105871)
/gene="ftsZ"
/locus_tag="b0095"
/gene_synonym="ECK0096; JW0093; sfiB; sulB"
/note="nucleotide binding site [chemical binding]; other
site"
/db_xref="CDD:100021"
misc_feature order(105920..105925,105929..105931,106109..106120,
106127..106129)
/gene="ftsZ"
/locus_tag="b0095"
/gene_synonym="ECK0096; JW0093; sfiB; sulB"
/note="SulA interaction site; other site"
/db_xref="CDD:100021"
gene 106557..107474
/gene="lpxC"
/locus_tag="b0096"
/gene_synonym="asmB; ECK0097; envA; JW0094"
/db_xref="EcoGene:EG10265"
/db_xref="GeneID:944816"
CDS 106557..107474
/gene="lpxC"
/locus_tag="b0096"
/gene_synonym="asmB; ECK0097; envA; JW0094"
/EC_number="3.5.1.-"
/function="enzyme; Surface polysaccharides & antigens"
/experiment="N-terminus verified by Edman degradation:
PMID 8530464"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0005737 - cytoplasm"
/GO_function="GO:0042280 - cell surface antigen activity,
host-interacting"
/GO_process="GO:0009245 - lipid A biosynthetic process"
/note="UDP-3-O-acyl N-acetylglucosamine deacetylase; lipid
A biosynthesis"
/codon_start=1
/transl_table=11
/product="UDP-3-O-acyl N-acetylglucosamine deacetylase"
/protein_id="NP_414638.1"
/db_xref="GI:16128089"
/db_xref="ASAP:ABE-0000336"
/db_xref="UniProtKB/Swiss-Prot:P0A725"
/db_xref="EcoGene:EG10265"
/db_xref="GeneID:944816"
/translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
misc_feature 106557..107453
/gene="lpxC"
/locus_tag="b0096"
/gene_synonym="asmB; ECK0097; envA; JW0094"
/note="UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine
deacetylase; Reviewed; Region: lpxC; PRK13186"
/db_xref="CDD:237294"
misc_feature 106560..107396
/gene="lpxC"
/locus_tag="b0096"
/gene_synonym="asmB; ECK0097; envA; JW0094"
/note="UDP-3-O-acyl N-acetylglycosamine deacetylase;
Region: LpxC; pfam03331"
/db_xref="CDD:202596"
gene 107705..108217
/gene="secM"
/locus_tag="b0097"
/gene_synonym="ECK0098; JW5007; srrA; yacA"
/db_xref="EcoGene:EG11087"
/db_xref="GeneID:944831"
CDS 107705..108217
/gene="secM"
/locus_tag="b0097"
/gene_synonym="ECK0098; JW5007; srrA; yacA"
/codon_start=1
/transl_table=11
/product="regulator of secA translation"
/protein_id="NP_414639.2"
/db_xref="GI:90111085"
/db_xref="ASAP:ABE-0000340"
/db_xref="UniProtKB/Swiss-Prot:P62395"
/db_xref="EcoGene:EG11087"
/db_xref="GeneID:944831"
/translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
GIRAGPQRLT"
misc_feature 107708..108211
/gene="secM"
/locus_tag="b0097"
/gene_synonym="ECK0098; JW5007; srrA; yacA"
/note="SecA regulator SecM; Provisional; Region: PRK02943"
/db_xref="CDD:235088"
gene 108279..110984
/gene="secA"
/locus_tag="b0098"
/gene_synonym="azi; ECK0099; JW0096; pea; prlD"
/db_xref="EcoGene:EG10936"
/db_xref="GeneID:944821"
CDS 108279..110984
/gene="secA"
/locus_tag="b0098"
/gene_synonym="azi; ECK0099; JW0096; pea; prlD"
/function="transport; Protein, peptide secretion"
/experiment="N-terminus verified by Edman degradation:
PMID 2841285"
/GO_component="GO:0005737 - cytoplasm"
/note="preprotein translocase; secretion protein"
/codon_start=1
/transl_table=11
/product="preprotein translocase subunit, ATPase"
/protein_id="NP_414640.1"
/db_xref="GI:16128091"
/db_xref="ASAP:ABE-0000343"
/db_xref="UniProtKB/Swiss-Prot:P10408"
/db_xref="EcoGene:EG10936"
/db_xref="GeneID:944821"
/translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
misc_feature 108282..110981
/gene="secA"
/locus_tag="b0098"
/gene_synonym="azi; ECK0099; JW0096; pea; prlD"
/note="preprotein translocase subunit SecA; Reviewed;
Region: PRK12904"
/db_xref="CDD:237259"
misc_feature 108960..109352
/gene="secA"
/locus_tag="b0098"
/gene_synonym="azi; ECK0099; JW0096; pea; prlD"
/note="SecA preprotein cross-linking domain; Region:
SecA_PP_bind; smart00958"
/db_xref="CDD:214938"
misc_feature 110919..110975
/gene="secA"
/locus_tag="b0098"
/gene_synonym="azi; ECK0099; JW0096; pea; prlD"
/note="SEC-C motif; Region: SEC-C; pfam02810"
/db_xref="CDD:202406"
gene 111044..111433
/gene="mutT"
/locus_tag="b0099"
/gene_synonym="ECK0100; JW0097; nudA"
/db_xref="EcoGene:EG10626"
/db_xref="GeneID:944824"
CDS 111044..111433
/gene="mutT"
/locus_tag="b0099"
/gene_synonym="ECK0100; JW0097; nudA"
/EC_number="3.6.1.-"
/function="enzyme; 2'-Deoxyribonucleotide metabolism"
/experiment="N-terminus verified by Edman degradation:
PMID 3288626"
/GO_component="GO:0005737 - cytoplasm"
/note="7,8-dihydro-8-oxoguanine-triphosphatase, prefers
dGTP, causes AT-GC transversions"
/codon_start=1
/transl_table=11
/product="nucleoside triphosphate pyrophosphohydrolase,
marked preference for dGTP"
/protein_id="NP_414641.1"
/db_xref="GI:16128092"
/db_xref="ASAP:ABE-0000346"
/db_xref="UniProtKB/Swiss-Prot:P08337"
/db_xref="EcoGene:EG10626"
/db_xref="GeneID:944824"
/translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVERWEGEPWGKEGQPG
EWMSLVGLNADDFPPANEPVIAKLKRL"
misc_feature 111053..111424
/gene="mutT"
/locus_tag="b0099"
/gene_synonym="ECK0100; JW0097; nudA"
/note="The MutT pyrophosphohydrolase is a prototypical
Nudix hydrolase that catalyzes the hydrolysis of
nucleoside and deoxynucleoside triphosphates (NTPs and
dNTPs) by substitution at a beta-phosphorus to yield a
nucleotide monophosphate (NMP) and inorganic...; Region:
MutT_pyrophosphohydrolase; cd03425"
/db_xref="CDD:239517"
misc_feature order(111053..111055,111059..111061,111155..111160,
111164..111166,111200..111202,111209..111214,
111281..111283,111335..111337,111398..111400)
/gene="mutT"
/locus_tag="b0099"
/gene_synonym="ECK0100; JW0097; nudA"
/note="active site"
/db_xref="CDD:239517"
misc_feature order(111053..111055,111059..111061,111155..111160,
111275..111277,111281..111283,111398..111400)
/gene="mutT"
/locus_tag="b0099"
/gene_synonym="ECK0100; JW0097; nudA"
/note="8-oxo-dGMP binding site [chemical binding]; other
site"
/db_xref="CDD:239517"
misc_feature 111155..111223
/gene="mutT"
/locus_tag="b0099"
/gene_synonym="ECK0100; JW0097; nudA"
/note="nudix motif; other site"
/db_xref="CDD:239517"
misc_feature order(111200..111202,111209..111214)
/gene="mutT"
/locus_tag="b0099"
/gene_synonym="ECK0100; JW0097; nudA"
/note="metal binding site [ion binding]; metal-binding
site"
/db_xref="CDD:239517"
repeat_region 111430..111629
/note="RIP8 (repetitive extragenic palindromic) element;
contains 4 REP sequences and 1 IHF site"
gene complement(111649..111846)
/gene="yacG"
/locus_tag="b0101"
/gene_synonym="ECK0101; JW5008"
/db_xref="EcoGene:EG12314"
/db_xref="GeneID:945630"
CDS complement(111649..111846)
/gene="yacG"
/locus_tag="b0101"
/gene_synonym="ECK0101; JW5008"
/codon_start=1
/transl_table=11
/product="DNA gyrase inhibitor"
/protein_id="NP_414643.1"
/db_xref="GI:16128094"
/db_xref="ASAP:ABE-0000351"
/db_xref="UniProtKB/Swiss-Prot:P0A8H8"
/db_xref="EcoGene:EG12314"
/db_xref="GeneID:945630"
/translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
KRIPSSGDLSESDDWSEEPKQ"
misc_feature complement(111661..111846)
/gene="yacG"
/locus_tag="b0101"
/gene_synonym="ECK0101; JW5008"
/note="DNA gyrase inhibitor; Reviewed; Region: PRK00418"
/db_xref="CDD:234754"
gene complement(111856..112599)
/gene="zapD"
/locus_tag="b0102"
/gene_synonym="ECK0102; JW0099; yacF"
/db_xref="EcoGene:EG12313"
/db_xref="GeneID:944873"
CDS complement(111856..112599)
/gene="zapD"
/locus_tag="b0102"
/gene_synonym="ECK0102; JW0099; yacF"
/codon_start=1
/transl_table=11
/product="FtsZ stabilizer"
/protein_id="NP_414644.1"
/db_xref="GI:16128095"
/db_xref="ASAP:ABE-0000355"
/db_xref="UniProtKB/Swiss-Prot:P36680"
/db_xref="EcoGene:EG12313"
/db_xref="GeneID:944873"
/translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
KSRFAIRFMPLDTENGQVPERLDFELACC"
misc_feature complement(111859..112590)
/gene="zapD"
/locus_tag="b0102"
/gene_synonym="ECK0102; JW0099; yacF"
/note="Uncharacterized protein conserved in bacteria
[Function unknown]; Region: COG4582"
/db_xref="CDD:226948"
gene complement(112599..113219)
/gene="coaE"
/locus_tag="b0103"
/gene_synonym="ECK0103; JW0100; yacE"
/db_xref="EcoGene:EG12312"
/db_xref="GeneID:949060"
CDS complement(112599..113219)
/gene="coaE"
/locus_tag="b0103"
/gene_synonym="ECK0103; JW0100; yacE"
/EC_number="2.7.1.24"
/function="orf; Not classified"
/note="putative DNA repair protein"
/codon_start=1
/transl_table=11
/product="dephospho-CoA kinase"
/protein_id="NP_414645.1"
/db_xref="GI:16128096"
/db_xref="ASAP:ABE-0000357"
/db_xref="UniProtKB/Swiss-Prot:P0A6I9"
/db_xref="EcoGene:EG12312"
/db_xref="GeneID:949060"
/translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
misc_feature complement(112617..113219)
/gene="coaE"
/locus_tag="b0103"
/gene_synonym="ECK0103; JW0100; yacE"
/note="Dephospho-CoA kinase [Coenzyme metabolism]; Region:
CoaE; COG0237"
/db_xref="CDD:223315"
misc_feature complement(112677..113210)
/gene="coaE"
/locus_tag="b0103"
/gene_synonym="ECK0103; JW0100; yacE"
/note="Dephospho-coenzyme A kinase (DPCK, EC 2.7.1.24)
catalyzes the phosphorylation of dephosphocoenzyme A
(dCoA) to yield CoA, which is the final step in CoA
biosynthesis; Region: DPCK; cd02022"
/db_xref="CDD:238980"
misc_feature complement(order(112743..112745,112878..112883,
112953..112955,113121..113123,113196..113198))
/gene="coaE"
/locus_tag="b0103"
/gene_synonym="ECK0103; JW0100; yacE"
/note="CoA-binding site [chemical binding]; other site"
/db_xref="CDD:238980"
misc_feature complement(order(112695..112697,112800..112802,
113175..113180,113187..113195))
/gene="coaE"
/locus_tag="b0103"
/gene_synonym="ECK0103; JW0100; yacE"
/note="ATP-binding [chemical binding]; other site"
/db_xref="CDD:238980"
gene 113444..114487
/gene="guaC"
/locus_tag="b0104"
/gene_synonym="ECK0104; JW0101"
/db_xref="EcoGene:EG10422"
/db_xref="GeneID:948986"
CDS 113444..114487
/gene="guaC"
/locus_tag="b0104"
/gene_synonym="ECK0104; JW0101"
/EC_number="1.7.1.7"
/function="enzyme; Purine ribonucleotide biosynthesis"
/experiment="N-terminus verified by Edman degradation:
PMID 9298646"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0009152 - purine ribonucleotide
biosynthetic process"
/GO_process="GO:0015949 - nucleobase, nucleoside and
nucleotide interconversion"
/codon_start=1
/transl_table=11
/product="GMP reductase"
/protein_id="NP_414646.1"
/db_xref="GI:16128097"
/db_xref="ASAP:ABE-0000362"
/db_xref="UniProtKB/Swiss-Prot:P60560"
/db_xref="EcoGene:EG10422"
/db_xref="GeneID:948986"
/translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
RVQEQENRIFNNL"
misc_feature 113444..114481
/gene="guaC"
/locus_tag="b0104"
/gene_synonym="ECK0104; JW0101"
/note="guanosine 5'-monophosphate oxidoreductase;
Provisional; Region: PRK05096"
/db_xref="CDD:235343"
misc_feature 113468..114451
/gene="guaC"
/locus_tag="b0104"
/gene_synonym="ECK0104; JW0101"
/note="IMPDH: The catalytic domain of the inosine
monophosphate dehydrogenase. IMPDH catalyzes the
NAD-dependent oxidation of inosine 5'-monophosphate (IMP)
to xanthosine 5' monophosphate (XMP). It is a
rate-limiting step in the de novo synthesis of...; Region:
IMPDH; cd00381"
/db_xref="CDD:238223"
misc_feature order(113597..113599,113993..114001,114098..114100,
114104..114106,114167..114172,114239..114241,
114245..114253,114308..114313)
/gene="guaC"
/locus_tag="b0104"
/gene_synonym="ECK0104; JW0101"
/note="active site"
/db_xref="CDD:238223"
gene complement(114522..115724)
/gene="hofC"
/locus_tag="b0106"
/gene_synonym="ECK0105; hopC; JW0102; yacD"
/db_xref="EcoGene:EG11798"
/db_xref="GeneID:945806"
CDS complement(114522..115724)
/gene="hofC"
/locus_tag="b0106"
/gene_synonym="ECK0105; hopC; JW0102; yacD"
/function="putative transport; Not classified"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/note="putative integral membrane protein involved in
biogenesis of fimbriae, protein transport, DNA uptake"
/codon_start=1
/transl_table=11
/product="assembly protein in type IV pilin biogenesis,
transmembrane protein"
/protein_id="NP_414648.1"
/db_xref="GI:16128099"
/db_xref="ASAP:ABE-0000366"
/db_xref="UniProtKB/Swiss-Prot:P36646"
/db_xref="EcoGene:EG11798"
/db_xref="GeneID:945806"
/translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGQPIWLALKNTGEFSPLCLQLVRTG
EASGSLDLMLDNLAHHHRENTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
GDAMSGMG"
misc_feature complement(114534..115724)
/gene="hofC"
/locus_tag="b0106"
/gene_synonym="ECK0105; hopC; JW0102; yacD"
/note="type IV pilin biogenesis protein; Provisional;
Region: PRK10573"
/db_xref="CDD:182559"
misc_feature complement(115227..115535)
/gene="hofC"
/locus_tag="b0106"
/gene_synonym="ECK0105; hopC; JW0102; yacD"
/note="Type II secretion system (T2SS), protein F; Region:
T2SF; pfam00482"
/db_xref="CDD:215939"
misc_feature complement(<114657..114929)
/gene="hofC"
/locus_tag="b0106"
/gene_synonym="ECK0105; hopC; JW0102; yacD"
/note="Type II secretion system (T2SS), protein F; Region:
T2SF; pfam00482"
/db_xref="CDD:215939"
gene complement(115714..117099)
/gene="hofB"
/locus_tag="b0107"
/gene_synonym="ECK0106; hopB; JW0103"
/db_xref="EcoGene:EG12106"
/db_xref="GeneID:947481"
CDS complement(115714..117099)
/gene="hofB"
/locus_tag="b0107"
/gene_synonym="ECK0106; hopB; JW0103"
/function="putative transport; Not classified"
/GO_component="GO:0009289 - pilus"
/note="putative integral membrane protein involved in
biogenesis of fimbriae, protein transport, DNA uptake"
/codon_start=1
/transl_table=11
/product="conserved protein with nucleoside triphosphate
hydrolase domain"
/protein_id="NP_414649.1"
/db_xref="GI:16128100"
/db_xref="ASAP:ABE-0000370"
/db_xref="UniProtKB/Swiss-Prot:P36645"
/db_xref="EcoGene:EG12106"
/db_xref="GeneID:947481"
/translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLV
LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
LIRVLGMPHGE"
misc_feature complement(115723..117099)
/gene="hofB"
/locus_tag="b0107"
/gene_synonym="ECK0106; hopB; JW0103"
/note="hypothetical protein; Provisional; Region:
PRK10436"
/db_xref="CDD:236694"
misc_feature complement(115915..116694)
/gene="hofB"
/locus_tag="b0107"
/gene_synonym="ECK0106; hopB; JW0103"
/note="PulE/GspE The type II secretory pathway is the main
terminal branch of the general secretory pathway (GSP).
It is responsible for the export the majority of
Gram-negative bacterial exoenzymes and toxins. PulE is a
cytoplasmic protein of the GSP, which...; Region:
PulE-GspE; cd01129"
/db_xref="CDD:238549"
misc_feature complement(116413..116436)
/gene="hofB"
/locus_tag="b0107"
/gene_synonym="ECK0106; hopB; JW0103"
/note="Walker A motif; other site"
/db_xref="CDD:238549"
misc_feature complement(order(116224..116229,116347..116352,
116356..116358,116413..116421,116431..116433))
/gene="hofB"
/locus_tag="b0107"
/gene_synonym="ECK0106; hopB; JW0103"
/note="ATP binding site [chemical binding]; other site"
/db_xref="CDD:238549"
misc_feature complement(116224..116241)
/gene="hofB"
/locus_tag="b0107"
/gene_synonym="ECK0106; hopB; JW0103"
/note="Walker B motif; other site"
/db_xref="CDD:238549"
gene complement(117109..117549)
/gene="ppdD"
/locus_tag="b0108"
/gene_synonym="ECK0107; JW0104"
/db_xref="EcoGene:EG12107"
/db_xref="GeneID:945817"
CDS complement(117109..117549)
/gene="ppdD"
/locus_tag="b0108"
/gene_synonym="ECK0107; JW0104"
/function="putative enzyme; Not classified"
/GO_component="GO:0009289 - pilus"
/note="prelipin peptidase dependent protein"
/codon_start=1
/transl_table=11
/product="putative major pilin subunit"
/protein_id="NP_414650.1"
/db_xref="GI:16128101"
/db_xref="ASAP:ABE-0000372"
/db_xref="UniProtKB/Swiss-Prot:P36647"
/db_xref="EcoGene:EG12107"
/db_xref="GeneID:945817"
/translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
misc_feature complement(117112..117549)
/gene="ppdD"
/locus_tag="b0108"
/gene_synonym="ECK0107; JW0104"
/note="putative major pilin subunit; Provisional; Region:
PRK10574"
/db_xref="CDD:236718"
misc_feature complement(<117505..>117543)
/gene="ppdD"
/locus_tag="b0108"
/gene_synonym="ECK0107; JW0104"
/note="Type IV pilin N-term methylation site GFxxxE;
Region: N_methyl_2; pfam13544"
/db_xref="CDD:205722"
misc_feature complement(117187..117402)
/gene="ppdD"
/locus_tag="b0108"
/gene_synonym="ECK0107; JW0104"
/note="Pilin (bacterial filament); Region: Pilin;
pfam00114"
/db_xref="CDD:215727"
gene complement(117752..118645)
/gene="nadC"
/locus_tag="b0109"
/gene_synonym="ECK0108; JW0105"
/db_xref="EcoGene:EG11546"
/db_xref="GeneID:948869"
CDS complement(117752..118645)
/gene="nadC"
/locus_tag="b0109"
/gene_synonym="ECK0108; JW0105"
/EC_number="2.4.2.19"
/function="enzyme; Biosynthesis of cofactors, carriers:
Pyridine nucleotide"
/GO_process="GO:0009435 - NAD biosynthetic process"
/codon_start=1
/transl_table=11
/product="quinolinate phosphoribosyltransferase"
/protein_id="NP_414651.1"
/db_xref="GI:16128102"
/db_xref="ASAP:ABE-0000374"
/db_xref="UniProtKB/Swiss-Prot:P30011"
/db_xref="EcoGene:EG11546"
/db_xref="GeneID:948869"
/translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
FISVGALTKHVQALDLSMRFR"
misc_feature complement(117755..118645)
/gene="nadC"
/locus_tag="b0109"
/gene_synonym="ECK0108; JW0105"
/note="quinolinate phosphoribosyltransferase; Validated;
Region: PRK09016"
/db_xref="CDD:181612"
misc_feature complement(117761..118570)
/gene="nadC"
/locus_tag="b0109"
/gene_synonym="ECK0108; JW0105"
/note="Quinolinate phosphoribosyl transferase (QAPRTase or
QPRTase), also called nicotinate-nucleotide
pyrophosphorylase, is involved in the de novo synthesis of
NAD in both prokaryotes and eukaryotes. It catalyses the
reaction of quinolinic acid (QA) with...; Region: QPRTase;
cd01572"
/db_xref="CDD:238806"
misc_feature complement(order(117791..117793,118037..118039,
118070..118072,118079..118084,118088..118090,
118094..118096,118106..118111,118166..118171,
118181..118183,118187..118189,118277..118279,
118289..118294,118505..118510))
/gene="nadC"
/locus_tag="b0109"
/gene_synonym="ECK0108; JW0105"
/note="dimerization interface [polypeptide binding]; other
site"
/db_xref="CDD:238806"
misc_feature complement(order(117791..117793,117800..117805,
117809..117811,117866..117871,117938..117940,
118118..118123,118184..118192))
/gene="nadC"
/locus_tag="b0109"
/gene_synonym="ECK0108; JW0105"
/note="active site"
/db_xref="CDD:238806"
gene 118733..119284
/gene="ampD"
/locus_tag="b0110"
/gene_synonym="ECK0109; JW0106"
/db_xref="EcoGene:EG10041"
/db_xref="GeneID:948877"
CDS 118733..119284
/gene="ampD"
/locus_tag="b0110"
/gene_synonym="ECK0109; JW0106"
/EC_number="3.5.1.28"
/function="regulator; Not classified"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0000270 - peptidoglycan metabolic process"
/note="regulates ampC"
/codon_start=1
/transl_table=11
/product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase,
Zn-dependent; murein amidase"
/protein_id="NP_414652.1"
/db_xref="GI:16128103"
/db_xref="ASAP:ABE-0000380"
/db_xref="UniProtKB/Swiss-Prot:P13016"
/db_xref="EcoGene:EG10041"
/db_xref="GeneID:948877"
/translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
RKTDPGPAFDWARFRVLVSKETT"
misc_feature 118739..>119257
/gene="ampD"
/locus_tag="b0110"
/gene_synonym="ECK0109; JW0106"
/note="N-acetyl-anhydromuramyl-L-alanine amidase [Cell
envelope biogenesis, outer membrane]; Region: ampD;
COG3023"
/db_xref="CDD:225567"
misc_feature 118814..119236
/gene="ampD"
/locus_tag="b0110"
/gene_synonym="ECK0109; JW0106"
/note="Peptidoglycan recognition proteins (PGRPs) are
pattern recognition receptors that bind, and in certain
cases, hydrolyze peptidoglycans (PGNs) of bacterial cell
walls. PGRPs have been divided into three classes: short
PGRPs (PGRP-S), that are small (20...; Region: PGRP;
cd06583"
/db_xref="CDD:133475"
misc_feature order(118832..118834,118952..118954,119192..119194,
119216..119218,119222..119224)
/gene="ampD"
/locus_tag="b0110"
/gene_synonym="ECK0109; JW0106"
/note="amidase catalytic site [active]"
/db_xref="CDD:133475"
misc_feature order(118832..118834,119192..119194,119222..119224)
/gene="ampD"
/locus_tag="b0110"
/gene_synonym="ECK0109; JW0106"
/note="Zn binding residues [ion binding]; other site"
/db_xref="CDD:133475"
misc_feature order(118835..118840,118910..118912,118952..118954,
118994..118996,119015..119020,119057..119059,
119192..119194,119204..119206,119216..119224)
/gene="ampD"
/locus_tag="b0110"
/gene_synonym="ECK0109; JW0106"
/note="substrate binding site [chemical binding]; other
site"
/db_xref="CDD:133475"
gene 119281..120135
/gene="ampE"
/locus_tag="b0111"
/gene_synonym="ECK0110; JW0107"
/db_xref="EcoGene:EG10042"
/db_xref="GeneID:946678"
CDS 119281..120135
/gene="ampE"
/locus_tag="b0111"
/gene_synonym="ECK0110; JW0107"
/function="regulator; Drug/analog sensitivity"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/GO_process="GO:0042493 - response to drug"
/note="regulates ampC"
/codon_start=1
/transl_table=11
/product="putative inner membrane protein"
/protein_id="NP_414653.1"
/db_xref="GI:16128104"
/db_xref="ASAP:ABE-0000382"
/db_xref="UniProtKB/Swiss-Prot:P0AE14"
/db_xref="EcoGene:EG10042"
/db_xref="GeneID:946678"
/translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
LTIYGALV"
misc_feature 119281..120132
/gene="ampE"
/locus_tag="b0111"
/gene_synonym="ECK0110; JW0107"
/note="regulatory protein AmpE; Provisional; Region:
PRK10987"
/db_xref="CDD:236811"
gene complement(120178..121551)
/gene="aroP"
/locus_tag="b0112"
/gene_synonym="ECK0111; JW0108"
/db_xref="EcoGene:EG10084"
/db_xref="GeneID:946018"
CDS complement(120178..121551)
/gene="aroP"
/locus_tag="b0112"
/gene_synonym="ECK0111; JW0108"
/function="transport; Transport of small molecules: Amino
acids, amines"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/GO_process="GO:0009094 - L-phenylalanine biosynthetic
process"
/GO_process="GO:0006571 - tyrosine biosynthetic process"
/note="aromatic amino acid transport protein"
/codon_start=1
/transl_table=11
/product="aromatic amino acid transporter"
/protein_id="NP_414654.1"
/db_xref="GI:16128105"
/db_xref="ASAP:ABE-0000384"
/db_xref="UniProtKB/Swiss-Prot:P15993"
/db_xref="EcoGene:EG10084"
/db_xref="GeneID:946018"
/translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
AKAVKAH"
misc_feature complement(120181..121548)
/gene="aroP"
/locus_tag="b0112"
/gene_synonym="ECK0111; JW0108"
/note="aromatic amino acid transporter; Provisional;
Region: PRK10238"
/db_xref="CDD:182324"
gene 122092..122856
/gene="pdhR"
/locus_tag="b0113"
/gene_synonym="aceC; ECK0112; genA; JW0109; yacB"
/db_xref="EcoGene:EG11088"
/db_xref="GeneID:944827"
CDS 122092..122856
/gene="pdhR"
/locus_tag="b0113"
/gene_synonym="aceC; ECK0112; genA; JW0109; yacB"
/function="regulator; Energy metabolism, carbon: Pyruvate
dehydrogenase"
/GO_component="GO:0005737 - cytoplasm"
/GO_function="GO:0016564 - transcription repressor
activity"
/GO_process="GO:0006096 - glycolysis"
/GO_process="GO:0006350 - transcription"
/note="transcriptional regulator for pyruvate
dehydrogenase complex"
/codon_start=1
/transl_table=11
/product="DNA-binding transcriptional dual regulator"
/protein_id="NP_414655.1"
/db_xref="GI:16128106"
/db_xref="ASAP:ABE-0000394"
/db_xref="UniProtKB/Swiss-Prot:P0ACL9"
/db_xref="EcoGene:EG11088"
/db_xref="GeneID:944827"
/translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
misc_feature 122092..122775
/gene="pdhR"
/locus_tag="b0113"
/gene_synonym="aceC; ECK0112; genA; JW0109; yacB"
/note="Transcriptional regulators [Transcription]; Region:
FadR; COG2186"
/db_xref="CDD:225097"
misc_feature 122119..122313
/gene="pdhR"
/locus_tag="b0113"
/gene_synonym="aceC; ECK0112; genA; JW0109; yacB"
/note="Winged helix-turn-helix (WHTH) DNA-binding domain
of the GntR family of transcriptional regulators; Region:
WHTH_GntR; cd07377"
/db_xref="CDD:153418"
misc_feature order(122119..122121,122125..122127,122194..122196,
122200..122205,122227..122241,122245..122250,
122257..122259,122287..122292,122296..122307)
/gene="pdhR"
/locus_tag="b0113"
/gene_synonym="aceC; ECK0112; genA; JW0109; yacB"
/note="DNA-binding site [nucleotide binding]; DNA binding
site"
/db_xref="CDD:153418"
misc_feature 122386..122769
/gene="pdhR"
/locus_tag="b0113"
/gene_synonym="aceC; ECK0112; genA; JW0109; yacB"
/note="This entry represents the C-terminal ligand binding
domain of many members of the GntR family; Region: FCD;
smart00895"
/db_xref="CDD:214892"
gene 123017..125680
/gene="aceE"
/locus_tag="b0114"
/gene_synonym="ECK0113; JW0110"
/db_xref="EcoGene:EG10024"
/db_xref="GeneID:944834"
CDS 123017..125680
/gene="aceE"
/locus_tag="b0114"
/gene_synonym="ECK0113; JW0110"
/EC_number="1.2.4.1"
/function="enzyme; Energy metabolism, carbon: Pyruvate
dehydrogenase"
/GO_process="GO:0016052 - carbohydrate catabolic process"
/GO_process="GO:0006086 - acetyl-CoA biosynthetic process
from pyruvate"
/GO_process="GO:0006096 - glycolysis"
/GO_process="GO:0009436 - glyoxylate catabolic process"
/GO_process="GO:0009061 - anaerobic respiration"
/note="pyruvate dehydrogenase (decarboxylase component)"
/codon_start=1
/transl_table=11
/product="pyruvate dehydrogenase, decarboxylase component
E1, thiamin-binding"
/protein_id="NP_414656.1"
/db_xref="GI:16128107"
/db_xref="ASAP:ABE-0000397"
/db_xref="UniProtKB/Swiss-Prot:P0AFG8"
/db_xref="EcoGene:EG10024"
/db_xref="GeneID:944834"
/translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
misc_feature 123017..125677
/gene="aceE"
/locus_tag="b0114"
/gene_synonym="ECK0113; JW0110"
/note="pyruvate dehydrogenase subunit E1; Reviewed;
Region: aceE; PRK09405"
/db_xref="CDD:236500"
mat_peptide 123020..125677
/gene="aceE"
/locus_tag="b0114"
/gene_synonym="ECK0113; JW0110"
/product="pyruvate dehydrogenase, decarboxylase component
E1, thiamin-binding"
/experiment="N-terminus verified by Edman degradation:
PMID 9298646"
misc_feature 123242..124399
/gene="aceE"
/locus_tag="b0114"
/gene_synonym="ECK0113; JW0110"
/note="Thiamine pyrophosphate (TPP) family, E1 of E. coli
PDC-like subfamily, TPP-binding module; composed of
proteins similar to the E1 component of the Escherichia
coli pyruvate dehydrogenase multienzyme complex (PDC). PDC
catalyzes the oxidative...; Region: TPP_E1_EcPDC_like;
cd02017"
/db_xref="CDD:238975"
misc_feature order(123320..123328,123515..123526,123545..123550,
123554..123556,123560..123562,123587..123589,
123593..123601,123719..123730,123737..123739,
123746..123748,123758..123760,123806..123814,
123824..123826,123848..123850,123857..123862,
123869..123871)
/gene="aceE"
/locus_tag="b0114"
/gene_synonym="ECK0113; JW0110"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:238975"
misc_feature order(123344..123346,123437..123439,123443..123445,
123593..123595,123599..123601,123707..123715,
123722..123724,123797..123799,123803..123805,
124193..124195)
/gene="aceE"
/locus_tag="b0114"
/gene_synonym="ECK0113; JW0110"
/note="TPP-binding site [chemical binding]; other site"
/db_xref="CDD:238975"
gene 125695..127587
/gene="aceF"
/locus_tag="b0115"
/gene_synonym="ECK0114; JW0111"
/db_xref="EcoGene:EG10025"
/db_xref="GeneID:944794"
CDS 125695..127587
/gene="aceF"
/locus_tag="b0115"
/gene_synonym="ECK0114; JW0111"
/EC_number="2.3.1.12"
/function="enzyme; Energy metabolism, carbon: Pyruvate
dehydrogenase"
/GO_process="GO:0016052 - carbohydrate catabolic process"
/GO_process="GO:0006086 - acetyl-CoA biosynthetic process
from pyruvate"
/GO_process="GO:0006096 - glycolysis"
/GO_process="GO:0009436 - glyoxylate catabolic process"
/GO_process="GO:0009061 - anaerobic respiration"
/note="pyruvate dehydrogenase (dihydrolipoyltransacetylase
component)"
/codon_start=1
/transl_table=11
/product="pyruvate dehydrogenase,
dihydrolipoyltransacetylase component E2"
/protein_id="NP_414657.1"
/db_xref="GI:16128108"
/db_xref="ASAP:ABE-0000400"
/db_xref="UniProtKB/Swiss-Prot:P06959"
/db_xref="EcoGene:EG10025"
/db_xref="GeneID:944794"
/translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
IRRLVM"
misc_feature 125695..127584
/gene="aceF"
/locus_tag="b0115"
/gene_synonym="ECK0114; JW0111"
/note="pyruvate dehydrogenase dihydrolipoyltransacetylase;
Validated; Region: aceF; PRK11854"
/db_xref="CDD:236999"
mat_peptide 125698..127584
/gene="aceF"
/locus_tag="b0115"
/gene_synonym="ECK0114; JW0111"
/product="pyruvate dehydrogenase,
dihydrolipoyltransacetylase component E2"
/experiment="N-terminus verified by Edman degradation:
PMID 9298646"
misc_feature 125701..125916
/gene="aceF"
/locus_tag="b0115"
/gene_synonym="ECK0114; JW0111"
/note="Lipoyl domain of the dihydrolipoyl acyltransferase
component (E2) of 2-oxo acid dehydrogenases. 2-oxo acid
dehydrogenase multienzyme complexes, like pyruvate
dehydrogenase (PDH), 2-oxoglutarate dehydrogenase (OGDH)
and branched-chain 2-oxo acid...; Region: lipoyl_domain;
cd06849"
/db_xref="CDD:133458"
misc_feature order(125785..125787,125806..125823,125833..125835)
/gene="aceF"
/locus_tag="b0115"
/gene_synonym="ECK0114; JW0111"
/note="E3 interaction surface; other site"
/db_xref="CDD:133458"
misc_feature 125815..125817
/gene="aceF"
/locus_tag="b0115"
/gene_synonym="ECK0114; JW0111"
/note="lipoyl attachment site [posttranslational
modification]; other site"
/db_xref="CDD:133458"
misc_feature 126016..126225
/gene="aceF"
/locus_tag="b0115"
/gene_synonym="ECK0114; JW0111"
/note="Lipoyl domain of the dihydrolipoyl acyltransferase
component (E2) of 2-oxo acid dehydrogenases. 2-oxo acid
dehydrogenase multienzyme complexes, like pyruvate
dehydrogenase (PDH), 2-oxoglutarate dehydrogenase (OGDH)
and branched-chain 2-oxo acid...; Region: lipoyl_domain;
cd06849"
/db_xref="CDD:133458"
misc_feature order(126094..126096,126115..126132,126142..126144)
/gene="aceF"
/locus_tag="b0115"
/gene_synonym="ECK0114; JW0111"
/note="E3 interaction surface; other site"
/db_xref="CDD:133458"
misc_feature 126124..126126
/gene="aceF"
/locus_tag="b0115"
/gene_synonym="ECK0114; JW0111"
/note="lipoyl attachment site [posttranslational
modification]; other site"
/db_xref="CDD:133458"
misc_feature 126322..126528
/gene="aceF"
/locus_tag="b0115"
/gene_synonym="ECK0114; JW0111"
/note="Lipoyl domain of the dihydrolipoyl acyltransferase
component (E2) of 2-oxo acid dehydrogenases. 2-oxo acid
dehydrogenase multienzyme complexes, like pyruvate
dehydrogenase (PDH), 2-oxoglutarate dehydrogenase (OGDH)
and branched-chain 2-oxo acid...; Region: lipoyl_domain;
cd06849"
/db_xref="CDD:133458"
misc_feature order(126397..126399,126418..126435,126445..126447)
/gene="aceF"
/locus_tag="b0115"
/gene_synonym="ECK0114; JW0111"
/note="E3 interaction surface; other site"
/db_xref="CDD:133458"
misc_feature 126427..126429
/gene="aceF"
/locus_tag="b0115"
/gene_synonym="ECK0114; JW0111"
/note="lipoyl attachment site [posttranslational
modification]; other site"
/db_xref="CDD:133458"
misc_feature 126673..126783
/gene="aceF"
/locus_tag="b0115"
/gene_synonym="ECK0114; JW0111"
/note="e3 binding domain; Region: E3_binding; pfam02817"
/db_xref="CDD:202412"
misc_feature 126940..127584
/gene="aceF"
/locus_tag="b0115"
/gene_synonym="ECK0114; JW0111"
/note="2-oxoacid dehydrogenases acyltransferase (catalytic
domain); Region: 2-oxoacid_dh; pfam00198"
/db_xref="CDD:215782"
gene 127912..129336
/gene="lpd"
/locus_tag="b0116"
/gene_synonym="dhl; ECK0115; JW0112; lpdA"
/db_xref="EcoGene:EG10543"
/db_xref="GeneID:944854"
CDS 127912..129336
/gene="lpd"
/locus_tag="b0116"
/gene_synonym="dhl; ECK0115; JW0112; lpdA"
/EC_number="1.8.1.4"
/function="enzyme; Energy metabolism, carbon: Pyruvate
dehydrogenase"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0016052 - carbohydrate catabolic process"
/GO_process="GO:0009063 - cellular amino acid catabolic
process"
/GO_process="GO:0006096 - glycolysis"
/GO_process="GO:0006086 - acetyl-CoA biosynthetic process
from pyruvate"
/GO_process="GO:0006099 - tricarboxylic acid cycle"
/GO_process="GO:0009060 - aerobic respiration"
/GO_process="GO:0009061 - anaerobic respiration"
/GO_process="GO:0009436 - glyoxylate catabolic process"
/note="lipoamide dehydrogenase (NADH); component of
2-oxodehydrogenase and pyruvate complexes; L-protein of
glycine cleavage complex"
/codon_start=1
/transl_table=11
/product="lipoamide dehydrogenase, E3 component is part of
three enzyme complexes"
/protein_id="NP_414658.1"
/db_xref="GI:16128109"
/db_xref="ASAP:ABE-0000404"
/db_xref="UniProtKB/Swiss-Prot:P0A9P0"
/db_xref="EcoGene:EG10543"
/db_xref="GeneID:944854"
/translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
SVGLAAEVFEGSITDLPNPKAKKK"
mat_peptide 127915..129333
/gene="lpd"
/locus_tag="b0116"
/gene_synonym="dhl; ECK0115; JW0112; lpdA"
/product="lipoamide dehydrogenase, E3 component is part of
three enzyme complexes"
/experiment="N-terminus verified by Edman degradation:
PMID 6352260,9298646"
misc_feature 127918..129327
/gene="lpd"
/locus_tag="b0116"
/gene_synonym="dhl; ECK0115; JW0112; lpdA"
/note="dihydrolipoamide dehydrogenase; Reviewed; Region:
PRK06467"
/db_xref="CDD:180579"
misc_feature 127942..>128046
/gene="lpd"
/locus_tag="b0116"
/gene_synonym="dhl; ECK0115; JW0112; lpdA"
/note="NAD(P)-binding Rossmann-like domain; Region:
NAD_binding_8; pfam13450"
/db_xref="CDD:205628"
misc_feature 128440..128679
/gene="lpd"
/locus_tag="b0116"
/gene_synonym="dhl; ECK0115; JW0112; lpdA"
/note="Pyridine nucleotide-disulphide oxidoreductase;
Region: Pyr_redox; pfam00070"
/db_xref="CDD:215691"
misc_feature 128950..129279
/gene="lpd"
/locus_tag="b0116"
/gene_synonym="dhl; ECK0115; JW0112; lpdA"
/note="Pyridine nucleotide-disulphide oxidoreductase,
dimerisation domain; Region: Pyr_redox_dim; pfam02852"
/db_xref="CDD:217252"
repeat_region 129347..129361
/note="REP9 (repetitive extragenic palindromic) element;
contains 1 REP sequences"
gene complement(129407..131260)
/gene="yacH"
/locus_tag="b0117"
/gene_synonym="ECK0116; JW0113"
/db_xref="EcoGene:EG12315"
/db_xref="GeneID:944868"
CDS complement(129407..131260)
/gene="yacH"
/locus_tag="b0117"
/gene_synonym="ECK0116; JW0113"
/function="putative membrane; Not classified"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/note="putative membrane protein"
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="NP_414659.1"
/db_xref="GI:16128110"
/db_xref="ASAP:ABE-0000406"
/db_xref="UniProtKB/Swiss-Prot:P36682"
/db_xref="EcoGene:EG12315"
/db_xref="GeneID:944868"
/translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
LAVSDAAAVTFPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDNDDYHHHDGGHRDGNGWQHN
GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
TQLPAPTRDSQRQAAANQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFHDFS
RRQPLTQQQKDAARQRYQSASPEQRQAVRERMQTNPKIQQRREAARERIQSASPEQRQ
AVREKMQTNPQNQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSAVCERLSERGARRLER"
misc_feature complement(130172..131044)
/gene="yacH"
/locus_tag="b0117"
/gene_synonym="ECK0116; JW0113"
/note="Protein of unknown function (DUF3300); Region:
DUF3300; pfam11737"
/db_xref="CDD:221195"
misc_feature complement(129626..>129868)
/gene="yacH"
/locus_tag="b0117"
/gene_synonym="ECK0116; JW0113"
/note="Protein of unknown function (DUF3106); Region:
DUF3106; pfam11304"
/db_xref="CDD:221069"
misc_feature complement(129488..>129751)
/gene="yacH"
/locus_tag="b0117"
/gene_synonym="ECK0116; JW0113"
/note="CpxP component of the bacterial Cpx-two-component
system and related proteins; Region: CpxP_like; cl01482"
/db_xref="CDD:242529"
gene 131615..134212
/gene="acnB"
/locus_tag="b0118"
/gene_synonym="ECK0117; JW0114; yacI; yacJ"
/db_xref="EcoGene:EG12316"
/db_xref="GeneID:944864"
CDS 131615..134212
/gene="acnB"
/locus_tag="b0118"
/gene_synonym="ECK0117; JW0114; yacI; yacJ"
/EC_number="4.2.1.3"
/EC_number="4.2.1.99"
/function="enzyme; Energy metabolism, carbon: TCA cycle"
/experiment="N-terminus verified by Edman degradation:
PMID 8932712,9298646"
/GO_process="GO:0006099 - tricarboxylic acid cycle"
/GO_process="GO:0009060 - aerobic respiration"
/GO_process="GO:0009061 - anaerobic respiration"
/note="aconitate hydrase B"
/codon_start=1
/transl_table=11
/product="bifunctional aconitate hydratase
2/2-methylisocitrate dehydratase"
/protein_id="NP_414660.1"
/db_xref="GI:16128111"
/db_xref="ASAP:ABE-0000411"
/db_xref="UniProtKB/Swiss-Prot:P36683"
/db_xref="EcoGene:EG12316"
/db_xref="GeneID:944864"
/translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
DGVIFQTAV"
misc_feature 131615..134152
/gene="acnB"
/locus_tag="b0118"
/gene_synonym="ECK0117; JW0114; yacI; yacJ"
/note="bifunctional aconitate hydratase
2/2-methylisocitrate dehydratase; Validated; Region:
PRK09238"
/db_xref="CDD:236424"
misc_feature 132125..132547
/gene="acnB"
/locus_tag="b0118"
/gene_synonym="ECK0117; JW0114; yacI; yacJ"
/note="Aconitase B swivel domain. Aconitate hydratase B is
involved in energy metabolism as part of the TCA cycle. It
catalyses the formation of cis-aconitate from citrate.
This is the aconitase swivel domain, which undergoes
swivelling conformational change in...; Region:
AcnB_Swivel; cd01576"
/db_xref="CDD:238808"
misc_feature 132344..132352
/gene="acnB"
/locus_tag="b0118"
/gene_synonym="ECK0117; JW0114; yacI; yacJ"
/note="substrate binding site [chemical binding]; other
site"
/db_xref="CDD:238808"
misc_feature 132764..134074
/gene="acnB"
/locus_tag="b0118"
/gene_synonym="ECK0117; JW0114; yacI; yacJ"
/note="Aconitate hydratase B catalyses the formation of
cis-aconitate from citrate as part of the TCA cycle;
Region: AcnB; cd01581"
/db_xref="CDD:153131"
misc_feature order(132854..132856,132863..132865,133103..133108,
133931..133933,133985..133987,134000..134002)
/gene="acnB"
/locus_tag="b0118"
/gene_synonym="ECK0117; JW0114; yacI; yacJ"
/note="substrate binding site [chemical binding]; other
site"
/db_xref="CDD:153131"
misc_feature order(133109..133111,133742..133744,133919..133921,
133928..133933,133982..133984)
/gene="acnB"
/locus_tag="b0118"
/gene_synonym="ECK0117; JW0114; yacI; yacJ"
/note="ligand binding site [chemical binding]; other site"
/db_xref="CDD:153131"
gene 134388..134750
/gene="yacL"
/locus_tag="b0119"
/gene_synonym="ECK0118; JW0115"
/db_xref="EcoGene:EG12605"
/db_xref="GeneID:944809"
CDS 134388..134750
/gene="yacL"
/locus_tag="b0119"
/gene_synonym="ECK0118; JW0115"
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="NP_414661.2"
/db_xref="GI:90111086"
/db_xref="ASAP:ABE-0000414"
/db_xref="UniProtKB/Swiss-Prot:P0A8E5"
/db_xref="EcoGene:EG12605"
/db_xref="GeneID:944809"
/translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
VEDFLQVVAAYRNFVQQK"
misc_feature 134388..134747
/gene="yacL"
/locus_tag="b0119"
/gene_synonym="ECK0118; JW0115"
/note="hypothetical protein; Provisional; Region:
PRK05248"
/db_xref="CDD:235372"
gene complement(134788..135582)
/gene="speD"
/locus_tag="b0120"
/gene_synonym="ECK0119; JW0116"
/db_xref="EcoGene:EG10962"
/db_xref="GeneID:947719"
CDS complement(134788..135582)
/gene="speD"
/locus_tag="b0120"
/gene_synonym="ECK0119; JW0116"
/EC_number="4.1.1.50"
/function="enzyme; Central intermediary metabolism:
Polyamine biosynthesis"
/experiment="N-terminus verified by Edman degradation:
PMID 3546296"
/GO_process="GO:0006596 - polyamine biosynthetic process"
/codon_start=1
/transl_table=11
/product="S-adenosylmethionine decarboxylase"
/protein_id="NP_414662.1"
/db_xref="GI:16128113"
/db_xref="ASAP:ABE-0000416"
/db_xref="UniProtKB/Swiss-Prot:P0A7F6"
/db_xref="EcoGene:EG10962"
/db_xref="GeneID:947719"
/translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
misc_feature complement(134791..135582)
/gene="speD"
/locus_tag="b0120"
/gene_synonym="ECK0119; JW0116"
/note="S-adenosylmethionine decarboxylase; Provisional;
Region: PRK05462"
/db_xref="CDD:235480"
gene complement(135598..136464)
/gene="speE"
/locus_tag="b0121"
/gene_synonym="ECK0120; JW0117"
/db_xref="EcoGene:EG10963"
/db_xref="GeneID:947726"
CDS complement(135598..136464)
/gene="speE"
/locus_tag="b0121"
/gene_synonym="ECK0120; JW0117"
/EC_number="2.5.1.16"
/function="enzyme; Central intermediary metabolism:
Polyamine biosynthesis"
/GO_process="GO:0006596 - polyamine biosynthetic process"
/note="spermidine synthase = putrescine
aminopropyltransferase"
/codon_start=1
/transl_table=11
/product="spermidine synthase (putrescine
aminopropyltransferase)"
/protein_id="NP_414663.1"
/db_xref="GI:16128114"
/db_xref="ASAP:ABE-0000418"
/db_xref="UniProtKB/Swiss-Prot:P09158"
/db_xref="EcoGene:EG10963"
/db_xref="GeneID:947726"
/translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
QYLQDALASQPS"
mat_peptide complement(135601..136461)
/gene="speE"
/locus_tag="b0121"
/gene_synonym="ECK0120; JW0117"
/product="spermidine synthase (putrescine
aminopropyltransferase)"
/experiment="N-terminus verified by Edman degradation:
PMID 3526348"
misc_feature complement(135613..136446)
/gene="speE"
/locus_tag="b0121"
/gene_synonym="ECK0120; JW0117"
/note="spermidine synthase; Provisional; Region: PRK00811"
/db_xref="CDD:234843"
misc_feature complement(135892..136224)
/gene="speE"
/locus_tag="b0121"
/gene_synonym="ECK0120; JW0117"
/note="S-adenosylmethionine-dependent methyltransferases
(SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes
that use S-adenosyl-L-methionine (SAM or AdoMet) as a
substrate for methyltransfer, creating the product
S-adenosyl-L-homocysteine (AdoHcy); Region: AdoMet_MTases;
cd02440"
/db_xref="CDD:100107"
misc_feature complement(order(135991..135993,136042..136050,
136138..136143,136195..136215))
/gene="speE"
/locus_tag="b0121"
/gene_synonym="ECK0120; JW0117"
/note="S-adenosylmethionine binding site [chemical
binding]; other site"
/db_xref="CDD:100107"
gene complement(136570..136917)
/gene="yacC"
/locus_tag="b0122"
/gene_synonym="ECK0121; gspS; JW0118"
/db_xref="EcoGene:EG11089"
/db_xref="GeneID:948472"
CDS complement(136570..136917)
/gene="yacC"
/locus_tag="b0122"
/gene_synonym="ECK0121; gspS; JW0118"
/codon_start=1
/transl_table=11
/product="conserved protein, PulS_OutS family"
/protein_id="NP_414664.4"
/db_xref="GI:90111087"
/db_xref="ASAP:ABE-0000423"
/db_xref="UniProtKB/Swiss-Prot:P0AA95"
/db_xref="EcoGene:EG11089"
/db_xref="GeneID:948472"
/translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
LARDSLSLLAYVK"
misc_feature complement(136603..>136833)
/gene="yacC"
/locus_tag="b0122"
/gene_synonym="ECK0121; gspS; JW0118"
/note="Bacterial chaperone lipoprotein (PulS_OutS);
Region: PulS_OutS; cl09898"
/db_xref="CDD:245186"
gene 137083..138633
/gene="cueO"
/locus_tag="b0123"
/gene_synonym="cuiD; ECK0122; JW0119; yacK"
/db_xref="EcoGene:EG12318"
/db_xref="GeneID:947736"
CDS 137083..138633
/gene="cueO"
/locus_tag="b0123"
/gene_synonym="cuiD; ECK0122; JW0119; yacK"
/codon_start=1
/transl_table=11
/product="multicopper oxidase (laccase)"
/protein_id="NP_414665.1"
/db_xref="GI:16128116"
/db_xref="ASAP:ABE-0000430"
/db_xref="UniProtKB/Swiss-Prot:P36649"
/db_xref="EcoGene:EG12318"
/db_xref="GeneID:947736"
/translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
GMMLGFTV"
misc_feature 137083..138630
/gene="cueO"
/locus_tag="b0123"
/gene_synonym="cuiD; ECK0122; JW0119; yacK"
/note="multicopper oxidase; Provisional; Region: PRK10965"
/db_xref="CDD:236810"
mat_peptide 137167..138630
/gene="cueO"
/locus_tag="b0123"
/gene_synonym="cuiD; ECK0122; JW0119; yacK"
/product="multicopper oxidase (laccase)"
/experiment="N-terminus verified by Edman degradation:
PMID 9298646"
misc_feature 137233..137580
/gene="cueO"
/locus_tag="b0123"
/gene_synonym="cuiD; ECK0122; JW0119; yacK"
/note="Multicopper oxidase; Region: Cu-oxidase_3;
pfam07732"
/db_xref="CDD:219542"
misc_feature <137776..>137952
/gene="cueO"
/locus_tag="b0123"
/gene_synonym="cuiD; ECK0122; JW0119; yacK"
/note="Multicopper oxidase; Region: Cu-oxidase; pfam00394"
/db_xref="CDD:215896"
misc_feature 138286..138630
/gene="cueO"
/locus_tag="b0123"
/gene_synonym="cuiD; ECK0122; JW0119; yacK"
/note="Multicopper oxidase; Region: Cu-oxidase_2;
pfam07731"
/db_xref="CDD:219541"
repeat_region 138699..138796
/note="RIP10 (repetitive extragenic palindromic) element;
contains 2 REP sequences and 1 IHF site"
gene complement(138835..141225)
/gene="gcd"
/locus_tag="b0124"
/gene_synonym="ECK0123; JW0120"
/db_xref="EcoGene:EG10369"
/db_xref="GeneID:944830"
CDS complement(138835..141225)
/gene="gcd"
/locus_tag="b0124"
/gene_synonym="ECK0123; JW0120"
/EC_number="1.1.5.2"
/function="enzyme; Degradation of small molecules: Carbon
compounds"
/experiment="N-terminus verified by Edman degradation:
PMID 8509415"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/GO_process="GO:0006006 - glucose metabolic process"
/GO_process="GO:0016052 - carbohydrate catabolic process"
/codon_start=1
/transl_table=11
/product="glucose dehydrogenase"
/protein_id="NP_414666.1"
/db_xref="GI:16128117"
/db_xref="ASAP:ABE-0000433"
/db_xref="UniProtKB/Swiss-Prot:P15877"
/db_xref="EcoGene:EG10369"
/db_xref="GeneID:944830"
/translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
misc_feature complement(138850..141129)
/gene="gcd"
/locus_tag="b0124"
/gene_synonym="ECK0123; JW0120"
/note="membrane-bound PQQ-dependent dehydrogenase,
glucose/quinate/shikimate family; Region: PQQ_membr_DH;
TIGR03074"
/db_xref="CDD:234101"
misc_feature complement(138853..140724)
/gene="gcd"
/locus_tag="b0124"
/gene_synonym="ECK0123; JW0120"
/note="Membrane-bound PQQ-dependent glucose dehydrogenase;
Region: PQQ_mGDH; cd10280"
/db_xref="CDD:199838"
misc_feature complement(order(138856..138858,138862..138864,
138991..138993,139000..139008,139015..139017,
139021..139023,139174..139176,139183..139191,
139198..139200,139204..139206,139699..139701,
139708..139716,139723..139725,139729..139731,
139861..139863,139870..139878,139885..139887,
139891..139893,140095..140097,140104..140112,
140119..140121,140125..140127,140293..140295,
140302..140310,140317..140319,140323..140325,
140482..140484,140491..140499,140506..140508,
140512..140514,140632..140634,140641..140649))
/gene="gcd"
/locus_tag="b0124"
/gene_synonym="ECK0123; JW0120"
/note="Trp docking motif [polypeptide binding]; other
site"
/db_xref="CDD:199838"
misc_feature complement(order(139090..139092,139747..139749,
140014..140016,140170..140175,140218..140220,
140428..140430,140443..140448,140575..140577))
/gene="gcd"
/locus_tag="b0124"
/gene_synonym="ECK0123; JW0120"
/note="putative active site [active]"
/db_xref="CDD:199838"
gene 141431..141967
/gene="hpt"
/locus_tag="b0125"
/gene_synonym="ECK0124; JW5009"
/db_xref="EcoGene:EG20098"
/db_xref="GeneID:946624"
CDS 141431..141967
/gene="hpt"
/locus_tag="b0125"
/gene_synonym="ECK0124; JW5009"
/EC_number="2.4.2.8"
/function="enzyme; Salvage of nucleosides and nucleotides"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0015949 - nucleobase, nucleoside and
nucleotide interconversion"
/codon_start=1
/transl_table=11
/product="hypoxanthine phosphoribosyltransferase"
/protein_id="NP_414667.4"
/db_xref="GI:90111088"
/db_xref="ASAP:ABE-0000440"
/db_xref="UniProtKB/Swiss-Prot:P0A9M2"
/db_xref="EcoGene:EG20098"
/db_xref="GeneID:946624"
/translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
AQRYRHLPYIGKVILLDE"
misc_feature 141482..141838
/gene="hpt"
/locus_tag="b0125"
/gene_synonym="ECK0124; JW5009"
/note="Phosphoribosyl transferase (PRT)-type I domain;
Region: PRTases_typeI; cd06223"
/db_xref="CDD:206754"
misc_feature order(141554..141556,141560..141562,141725..141733,
141737..141751,141821..141823)
/gene="hpt"
/locus_tag="b0125"
/gene_synonym="ECK0124; JW5009"
/note="active site"
/db_xref="CDD:206754"
repeat_region 141978..141998
/note="REP11 (repetitive extragenic palindromic) element;
contains 1 REP sequences"
gene complement(142008..142670)
/gene="can"
/locus_tag="b0126"
/gene_synonym="ECK0125; JW0122; yadF"
/db_xref="EcoGene:EG12319"
/db_xref="GeneID:944832"
CDS complement(142008..142670)
/gene="can"
/locus_tag="b0126"
/gene_synonym="ECK0125; JW0122; yadF"
/EC_number="4.2.1.1"
/function="enzyme; Central intermediary metabolism: Pool,
multipurpose conversions"
/note="putative carbonic anhdrase"
/codon_start=1
/transl_table=11
/product="carbonic anhydrase"
/protein_id="NP_414668.1"
/db_xref="GI:16128119"
/db_xref="ASAP:ABE-0000442"
/db_xref="UniProtKB/Swiss-Prot:P61517"
/db_xref="EcoGene:EG12319"
/db_xref="GeneID:944832"
/translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
HK"
misc_feature complement(142098..142640)
/gene="can"
/locus_tag="b0126"
/gene_synonym="ECK0125; JW0122; yadF"
/note="Carbonic anhydrases (CA) are zinc-containing
enzymes that catalyze the reversible hydration of carbon
dioxide in a two-step mechanism in which the nucleophilic
attack of a zinc-bound hydroxide ion on carbon dioxide is
followed by the regeneration of an...; Region:
beta_CA_cladeA; cd00883"
/db_xref="CDD:238448"
misc_feature complement(order(142128..142130,142368..142370,
142377..142379,142407..142409,142422..142424,
142488..142490,142497..142499,142533..142541,
142545..142547,142566..142568,142572..142574))
/gene="can"
/locus_tag="b0126"
/gene_synonym="ECK0125; JW0122; yadF"
/note="active site clefts [active]"
/db_xref="CDD:238448"
misc_feature complement(order(142368..142370,142377..142379,
142539..142541,142545..142547))
/gene="can"
/locus_tag="b0126"
/gene_synonym="ECK0125; JW0122; yadF"
/note="zinc binding site [ion binding]; other site"
/db_xref="CDD:238448"
misc_feature complement(order(142107..142109,142113..142115,
142119..142124,142128..142130,142314..142316,
142422..142427,142434..142439,142479..142481,
142485..142487,142491..142499,142512..142514,
142518..142523,142527..142529,142533..142544))
/gene="can"
/locus_tag="b0126"
/gene_synonym="ECK0125; JW0122; yadF"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:238448"
gene 142779..143705
/gene="yadG"
/locus_tag="b0127"
/gene_synonym="ECK0126; JW0123"
/db_xref="EcoGene:EG12320"
/db_xref="GeneID:944833"
CDS 142779..143705
/gene="yadG"
/locus_tag="b0127"
/gene_synonym="ECK0126; JW0123"
/function="putative transport; Not classified"
/GO_component="GO:0005737 - cytoplasm"
/note="putative ATP-binding component of a transport
system"
/codon_start=1
/transl_table=11
/product="putative transporter subunit: ATP-binding
component of ABC superfamily"
/protein_id="NP_414669.1"
/db_xref="GI:16128120"
/db_xref="ASAP:ABE-0000447"
/db_xref="UniProtKB/Swiss-Prot:P36879"
/db_xref="EcoGene:EG12320"
/db_xref="GeneID:944833"
/translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
misc_feature 142779..143684
/gene="yadG"
/locus_tag="b0127"
/gene_synonym="ECK0126; JW0123"
/note="ABC-type multidrug transport system, ATPase
component [Defense mechanisms]; Region: CcmA; COG1131"
/db_xref="CDD:224054"
misc_feature 142791..143420
/gene="yadG"
/locus_tag="b0127"
/gene_synonym="ECK0126; JW0123"
/note="ATP-binding cassette domain of the drug resistance
transporter and related proteins, subfamily A; Region:
ABC_DR_subfamily_A; cd03230"
/db_xref="CDD:213197"
misc_feature 142890..142913
/gene="yadG"
/locus_tag="b0127"
/gene_synonym="ECK0126; JW0123"
/note="Walker A/P-loop; other site"
/db_xref="CDD:213197"
misc_feature order(142899..142904,142908..142916,143031..143033,
143259..143264,143358..143360)
/gene="yadG"
/locus_tag="b0127"
/gene_synonym="ECK0126; JW0123"
/note="ATP binding site [chemical binding]; other site"
/db_xref="CDD:213197"
misc_feature 143022..143033
/gene="yadG"
/locus_tag="b0127"
/gene_synonym="ECK0126; JW0123"
/note="Q-loop/lid; other site"
/db_xref="CDD:213197"
misc_feature 143187..143216
/gene="yadG"
/locus_tag="b0127"
/gene_synonym="ECK0126; JW0123"
/note="ABC transporter signature motif; other site"
/db_xref="CDD:213197"
misc_feature 143247..143264
/gene="yadG"
/locus_tag="b0127"
/gene_synonym="ECK0126; JW0123"
/note="Walker B; other site"
/db_xref="CDD:213197"
misc_feature 143271..143282
/gene="yadG"
/locus_tag="b0127"
/gene_synonym="ECK0126; JW0123"
/note="D-loop; other site"
/db_xref="CDD:213197"
misc_feature 143346..143366
/gene="yadG"
/locus_tag="b0127"
/gene_synonym="ECK0126; JW0123"
/note="H-loop/switch region; other site"
/db_xref="CDD:213197"
gene 143702..144472
/gene="yadH"
/locus_tag="b0128"
/gene_synonym="ECK0127; JW0124"
/db_xref="EcoGene:EG12321"
/db_xref="GeneID:944836"
CDS 143702..144472
/gene="yadH"
/locus_tag="b0128"
/gene_synonym="ECK0127; JW0124"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/codon_start=1
/transl_table=11
/product="putative transporter subunit: membrane component
of ABC superfamily"
/protein_id="NP_414670.1"
/db_xref="GI:16128121"
/db_xref="ASAP:ABE-0000450"
/db_xref="UniProtKB/Swiss-Prot:P0AFN6"
/db_xref="EcoGene:EG12321"
/db_xref="GeneID:944836"
/translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
misc_feature 143702..144469
/gene="yadH"
/locus_tag="b0128"
/gene_synonym="ECK0127; JW0124"
/note="inner membrane transport permease; Provisional;
Region: PRK15066"
/db_xref="CDD:237896"
misc_feature 143702..144466
/gene="yadH"
/locus_tag="b0128"
/gene_synonym="ECK0127; JW0124"
/note="ABC-type multidrug transport system, permease
component [Defense mechanisms]; Region: COG0842"
/db_xref="CDD:223912"
gene 144577..145017
/gene="yadI"
/locus_tag="b0129"
/gene_synonym="agaX; ECK0128; JW0125"
/db_xref="EcoGene:EG12322"
/db_xref="GeneID:947397"
CDS 144577..145017
/gene="yadI"
/locus_tag="b0129"
/gene_synonym="agaX; ECK0128; JW0125"
/function="putative transport; Not classified"
/GO_process="GO:0009401 - phosphoenolpyruvate-dependent
sugar phosphotransferase system"
/note="putative PTS enzyme II B component"
/codon_start=1
/transl_table=11
/product="putative PTS Enzyme IIA"
/protein_id="NP_414671.1"
/db_xref="GI:16128122"
/db_xref="ASAP:ABE-0000453"
/db_xref="UniProtKB/Swiss-Prot:P36881"
/db_xref="EcoGene:EG12322"
/db_xref="GeneID:947397"
/translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
misc_feature 144583..144936
/gene="yadI"
/locus_tag="b0129"
/gene_synonym="agaX; ECK0128; JW0125"
/note="PTS_IIA, PTS system, mannose/sorbose specific IIA
subunit. The bacterial phosphoenolpyruvate: sugar
phosphotransferase system (PTS) is a multi-protein system
involved in the regulation of a variety of metabolic and
transcriptional processes. This family...; Region:
PTS_IIA_man; cd00006"
/db_xref="CDD:237978"
misc_feature order(144601..144603,144643..144648,144679..144681,
144787..144789,144880..144882)
/gene="yadI"
/locus_tag="b0129"
/gene_synonym="agaX; ECK0128; JW0125"
/note="active pocket/dimerization site"
/db_xref="CDD:237978"
misc_feature order(144601..144603,144772..144774,144787..144789)
/gene="yadI"
/locus_tag="b0129"
/gene_synonym="agaX; ECK0128; JW0125"
/note="active site"
/db_xref="CDD:237978"
misc_feature 144601..144603
/gene="yadI"
/locus_tag="b0129"
/gene_synonym="agaX; ECK0128; JW0125"
/note="phosphorylation site [posttranslational
modification]"
/db_xref="CDD:237978"
gene 145081..146310
/gene="yadE"
/locus_tag="b0130"
/gene_synonym="ECK0129; JW0126"
/db_xref="EcoGene:EG11749"
/db_xref="GeneID:946536"
CDS 145081..146310
/gene="yadE"
/locus_tag="b0130"
/gene_synonym="ECK0129; JW0126"
/note="conserved hypothetical protein"
/codon_start=1
/transl_table=11
/product="putative polysaccharide deacetylase lipoprotein"
/protein_id="NP_414672.1"
/db_xref="GI:16128123"
/db_xref="ASAP:ABE-0000457"
/db_xref="UniProtKB/Swiss-Prot:P31666"
/db_xref="EcoGene:EG11749"
/db_xref="GeneID:946536"
/translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
ALDAQPDNGLSVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
LAQFNPHVWYLSYPFGGFNDNAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
TDSLETMSRLVSNQPQG"
misc_feature 145768..146277
/gene="yadE"
/locus_tag="b0130"
/gene_synonym="ECK0129; JW0126"
/note="Putative catalytic polysaccharide deacetylase
domain of uncharacterized protein yadE and similar
proteins; Region: CE4_yadE_5s; cd10966"
/db_xref="CDD:213024"
misc_feature order(145795..145800,145981..145983,145996..145998,
146122..146130)
/gene="yadE"
/locus_tag="b0130"
/gene_synonym="ECK0129; JW0126"
/note="putative active site [active]"
/db_xref="CDD:213024"
misc_feature order(145798..145800,145981..145983,145996..145998)
/gene="yadE"
/locus_tag="b0130"
/gene_synonym="ECK0129; JW0126"
/note="putative metal binding site [ion binding]; other
site"
/db_xref="CDD:213024"
gene complement(146314..146694)
/gene="panD"
/locus_tag="b0131"
/gene_synonym="ECK0130; JW0127"
/db_xref="EcoGene:EG11747"
/db_xref="GeneID:945686"
CDS complement(146314..146694)
/gene="panD"
/locus_tag="b0131"
/gene_synonym="ECK0130; JW0127"
/EC_number="4.1.1.11"
/function="enzyme; Biosynthesis of cofactors, carriers:
Pantothenate"
/experiment="N-terminus verified by Edman degradation:
PMID 9169598"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0015937 - coenzyme A biosynthetic process"
/codon_start=1
/transl_table=11
/product="aspartate 1-decarboxylase"
/protein_id="NP_414673.1"
/db_xref="GI:16128124"
/db_xref="ASAP:ABE-0000459"
/db_xref="UniProtKB/Swiss-Prot:P0A790"
/db_xref="EcoGene:EG11747"
/db_xref="GeneID:945686"
/translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
PNVAYFEGDNEMKRTAKAIPVQVA"
misc_feature complement(146359..146688)
/gene="panD"
/locus_tag="b0131"
/gene_synonym="ECK0130; JW0127"
/note="Aspartate alpha-decarboxylase or L-aspartate
1-decarboxylase, a pyruvoyl group-dependent decarboxylase
in beta-alanine production; Region: Asp_decarbox; cd06919"
/db_xref="CDD:132994"
misc_feature complement(order(146392..146394,146419..146427,
146437..146439,146461..146466,146470..146475,
146521..146535,146539..146541,146548..146550,
146554..146556,146566..146571,146578..146580,
146584..146586,146626..146637,146659..146664,
146668..146670,146674..146688))
/gene="panD"
/locus_tag="b0131"
/gene_synonym="ECK0130; JW0127"
/note="tetramerization interface [polypeptide binding];
other site"
/db_xref="CDD:132994"
misc_feature complement(order(146521..146523,146620..146625,
146662..146664,146668..146670))
/gene="panD"
/locus_tag="b0131"
/gene_synonym="ECK0130; JW0127"
/note="active site"
/db_xref="CDD:132994"
gene 146968..147870
/gene="yadD"
/locus_tag="b0132"
/gene_synonym="ECK0131; JW5010"
/db_xref="EcoGene:EG11748"
/db_xref="GeneID:944781"
CDS 146968..147870
/gene="yadD"
/locus_tag="b0132"
/gene_synonym="ECK0131; JW5010"
/codon_start=1
/transl_table=11
/product="putative transposase"
/protein_id="NP_414674.1"
/db_xref="GI:16128125"
/db_xref="ASAP:ABE-0000463"
/db_xref="UniProtKB/Swiss-Prot:P31665"
/db_xref="EcoGene:EG11748"
/db_xref="GeneID:944781"
/translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEVSQEFAQRLLSKG
MSREDVAEMANLPLAEIDKVINLI"
misc_feature 146986..147846
/gene="yadD"
/locus_tag="b0132"
/gene_synonym="ECK0131; JW5010"
/note="Uncharacterized conserved protein [Function
unknown]; Region: COG5464"
/db_xref="CDD:227751"
misc_feature 146986..147591
/gene="yadD"
/locus_tag="b0132"
/gene_synonym="ECK0131; JW5010"
/note="Putative transposase, YhgA-like; Region:
Transposase_31; pfam04754"
/db_xref="CDD:218244"
gene complement(147944..148795)
/gene="panC"
/locus_tag="b0133"
/gene_synonym="ECK0132; JW0129"
/db_xref="EcoGene:EG11746"
/db_xref="GeneID:944958"
CDS complement(147944..148795)
/gene="panC"
/locus_tag="b0133"
/gene_synonym="ECK0132; JW0129"
/EC_number="6.3.2.1"
/function="enzyme; Biosynthesis of cofactors, carriers:
Pantothenate"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0015937 - coenzyme A biosynthetic process"
/codon_start=1
/transl_table=11
/product="pantothenate synthetase"
/protein_id="NP_414675.1"
/db_xref="GI:16128126"
/db_xref="ASAP:ABE-0000465"
/db_xref="UniProtKB/Swiss-Prot:P31663"
/db_xref="EcoGene:EG11746"
/db_xref="GeneID:944958"
/translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
NKMVELA"
misc_feature complement(147956..148795)
/gene="panC"
/locus_tag="b0133"
/gene_synonym="ECK0132; JW0129"
/note="pantoate--beta-alanine ligase; Region: panC;
TIGR00018"
/db_xref="CDD:232784"
misc_feature complement(147965..148795)
/gene="panC"
/locus_tag="b0133"
/gene_synonym="ECK0132; JW0129"
/note="Pantoate-beta-alanine ligase; Region: PanC;
cd00560"
/db_xref="CDD:185673"
misc_feature complement(order(148229..148240,148262..148270,
148331..148333,148340..148345,148349..148354,
148394..148399,148406..148408,148583..148585,
148613..148615,148622..148624,148676..148678,
148685..148690,148694..148696,148703..148714))
/gene="panC"
/locus_tag="b0133"
/gene_synonym="ECK0132; JW0129"
/note="active site"
/db_xref="CDD:185673"
misc_feature complement(order(148238..148243,148262..148267,
148331..148333,148340..148345,148349..148354,
148394..148399,148613..148615,148676..148678,
148685..148687,148694..148696,148706..148714))
/gene="panC"
/locus_tag="b0133"
/gene_synonym="ECK0132; JW0129"
/note="ATP-binding site [chemical binding]; other site"
/db_xref="CDD:185673"
misc_feature complement(order(148331..148333,148385..148387,
148394..148399,148613..148615,148622..148624,
148706..148708,148712..148714))
/gene="panC"
/locus_tag="b0133"
/gene_synonym="ECK0132; JW0129"
/note="pantoate-binding site; other site"
/db_xref="CDD:185673"
misc_feature complement(148685..148696)
/gene="panC"
/locus_tag="b0133"
/gene_synonym="ECK0132; JW0129"
/note="HXXH motif; other site"
/db_xref="CDD:185673"
gene complement(148807..149601)
/gene="panB"
/locus_tag="b0134"
/gene_synonym="ECK0133; JW0130; panA"
/db_xref="EcoGene:EG11675"
/db_xref="GeneID:944839"
CDS complement(148807..149601)
/gene="panB"
/locus_tag="b0134"
/gene_synonym="ECK0133; JW0130; panA"
/EC_number="2.1.2.11"
/function="enzyme; Biosynthesis of cofactors, carriers:
Pantothenate"
/experiment="N-terminus verified by Edman degradation:
PMID 8096212,9298646"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0015937 - coenzyme A biosynthetic process"
/codon_start=1
/transl_table=11
/product="3-methyl-2-oxobutanoate
hydroxymethyltransferase"
/protein_id="NP_414676.1"
/db_xref="GI:16128127"
/db_xref="ASAP:ABE-0000467"
/db_xref="UniProtKB/Swiss-Prot:P31057"
/db_xref="EcoGene:EG11675"
/db_xref="GeneID:944839"
/translation="MKPTTISLLQKYKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
misc_feature complement(148831..149586)
/gene="panB"
/locus_tag="b0134"
/gene_synonym="ECK0133; JW0130; panA"
/note="Ketopantoate hydroxymethyltransferase (KPHMT) is
the first enzyme in the pantothenate biosynthesis pathway.
Ketopantoate hydroxymethyltransferase (KPHMT) catalyzes
the first committed step in the biosynthesis of
pantothenate (vitamin B5), which is a...; Region:
KPHMT-like; cd06557"
/db_xref="CDD:119342"
misc_feature complement(order(148879..148893,148900..148902,
148942..148947,148954..148956,149074..149076,
149158..149169,149173..149178,149206..149223,
149275..149277,149287..149292,149299..149301,
149311..149313,149338..149343,149380..149382,
149389..149391,149398..149403,149410..149412,
149425..149430,149440..149457,149461..149463,
149506..149508,149515..149526,149581..149586))
/gene="panB"
/locus_tag="b0134"
/gene_synonym="ECK0133; JW0130; panA"
/note="oligomerization interface [polypeptide binding];
other site"
/db_xref="CDD:119342"
misc_feature complement(order(148960..148962,148966..148968,
149059..149061,149179..149181,149194..149196,
149266..149268,149350..149352,149464..149472,
149476..149478,149533..149535))
/gene="panB"
/locus_tag="b0134"
/gene_synonym="ECK0133; JW0130; panA"
/note="active site"
/db_xref="CDD:119342"
misc_feature complement(order(149266..149268,149350..149352,
149467..149469))
/gene="panB"
/locus_tag="b0134"
/gene_synonym="ECK0133; JW0130; panA"
/note="metal binding site [ion binding]; metal-binding
site"
/db_xref="CDD:119342"
gene complement(149715..150953)
/gene="yadC"
/locus_tag="b0135"
/gene_synonym="ECK0134; JW0131"
/db_xref="EcoGene:EG11678"
/db_xref="GeneID:944837"
CDS complement(149715..150953)
/gene="yadC"
/locus_tag="b0135"
/gene_synonym="ECK0134; JW0131"
/function="putative structure; Not classified"
/GO_component="GO:0009289 - pilus"
/note="putative fimbrial-like protein"
/codon_start=1
/transl_table=11
/product="putative fimbrial-like adhesin protein"
/protein_id="NP_414677.1"
/db_xref="GI:16128128"
/db_xref="ASAP:ABE-0000470"
/db_xref="UniProtKB/Swiss-Prot:P31058"
/db_xref="EcoGene:EG11678"
/db_xref="GeneID:944837"
/translation="MKTIFRYILFLALYSCCNTVSAYTSFIVGNNAGVDNYRGPSTAA
QMTFNYTSTASNLVFYKPTQLGPTGVKMYWSYLDTGTGGGILYCNTSGRANPGPITIE
NAMVYSGKDYGGHKLFNTSVPGLYYTMLISRVWSAYDTITDIQSPGIYIGDPSNQEFF
FSVTDSDLQTKGCNKADDYDKFWAIGGIVHNITVEFYTDTNFDPTLNQQVQLSSSSNY
LYSFKAYSPGTKVVDHSNHIYVNFTLNNVKLTLPTCFTSILTGPSVNGSTVRMGEYSS
GTIKNGASPVPFDISLQNCIRVRNIETKLVTGKVGTQNTQLLGNTLTGSTAAKGVGVL
IEGLATSKNPLMTLKPNDTNSVYIDYETEDDTSDGVYPNQGNGTSQPLHFQATLKQDG
NIAIEPGEFKATSTFQVTYP"
misc_feature complement(149718..150953)
/gene="yadC"
/locus_tag="b0135"
/gene_synonym="ECK0134; JW0131"
/note="putative fimbrial-like adhesin protein;
Provisional; Region: PRK09723"
/db_xref="CDD:236617"
gene complement(151003..151599)
/gene="yadK"
/locus_tag="b0136"
/gene_synonym="ECK0135; JW0132"
/db_xref="EcoGene:EG12325"
/db_xref="GeneID:944835"
CDS complement(151003..151599)
/gene="yadK"
/locus_tag="b0136"
/gene_synonym="ECK0135; JW0132"
/function="orf; Not classified"
/note="putative fimbrial protein"
/codon_start=1
/transl_table=11
/product="putative fimbrial-like adhesin protein"
/protein_id="NP_414678.1"
/db_xref="GI:16128129"
/db_xref="ASAP:ABE-0000472"
/db_xref="UniProtKB/Swiss-Prot:P37016"
/db_xref="EcoGene:EG12325"
/db_xref="GeneID:944835"
/translation="MHPTQRKLMKRIILFLSLLFCIACPAIAGQDIDLVANVKNSTCK
SGISNQGNIDLGVVGVGYFSGNVTPESYQPGGKEFTITVSDCALQGTGDVLNQLHIDF
RALSGVMAAGSRQIFANEISSGASNVGVVIFSTQDSANTFNVLNASGGSRSVYPVMSD
DMNGSSWKFSTRMQKIDPALSVTSGQLMSHVLVDIYYE"
misc_feature complement(151006..151590)
/gene="yadK"
/locus_tag="b0136"
/gene_synonym="ECK0135; JW0132"
/note="putative fimbrial protein StaF; Provisional;
Region: PRK15262"
/db_xref="CDD:237933"
gene complement(151626..152231)
/gene="yadL"
/locus_tag="b0137"
/gene_synonym="ECK0136; JW0133"
/db_xref="EcoGene:EG12326"
/db_xref="GeneID:944829"
CDS complement(151626..152231)
/gene="yadL"
/locus_tag="b0137"
/gene_synonym="ECK0136; JW0133"
/function="orf; Not classified"
/note="putative fimbrial protein"
/codon_start=1
/transl_table=11
/product="putative fimbrial-like adhesin protein"
/protein_id="NP_414679.1"
/db_xref="GI:16128130"
/db_xref="ASAP:ABE-0000475"
/db_xref="UniProtKB/Swiss-Prot:P37017"
/db_xref="EcoGene:EG12326"
/db_xref="GeneID:944829"
/translation="MMTFKNLRYGLSSSVVLAASLFSVLSYAATDSIGLTVITTVEMG
TCTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKR
ATCEGTANDGAGFANGSTAADKASAVAVEVWSTVTPATGSATQFSCVTPASQEVTIST
AANAVVYYPMSARLVVEKNKTVNNVTAGKFSAPATFTVTYN"
misc_feature complement(151629..152144)
/gene="yadL"
/locus_tag="b0137"
/gene_synonym="ECK0136; JW0133"
/note="putative fimbrial protein StaE; Provisional;
Region: PRK15263"
/db_xref="CDD:185171"
gene complement(152243..152812)
/gene="yadM"
/locus_tag="b0138"
/gene_synonym="ECK0137; JW0134"
/db_xref="EcoGene:EG12327"
/db_xref="GeneID:944828"
CDS complement(152243..152812)
/gene="yadM"
/locus_tag="b0138"
/gene_synonym="ECK0137; JW0134"
/function="putative structure; Not classified"
/GO_component="GO:0009289 - pilus"
/note="putative fimbrial-like protein"
/codon_start=1
/transl_table=11
/product="putative fimbrial-like adhesin protein"
/protein_id="NP_414680.4"
/db_xref="GI:90111089"
/db_xref="ASAP:ABE-0000477"
/db_xref="UniProtKB/Swiss-Prot:P37018"
/db_xref="EcoGene:EG12327"
/db_xref="GeneID:944828"
/translation="MIKTTPHKIVILMGILLSPSVFATDINVEFTATVKATTCNITLT
GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
MTVALRETDAGQGVPGNFRALATFNFIYQ"
misc_feature complement(152246..152746)
/gene="yadM"
/locus_tag="b0138"
/gene_synonym="ECK0137; JW0134"
/note="Fimbrial protein; Region: Fimbrial; cl01416"
/db_xref="CDD:242492"
gene complement(152829..155426)
/gene="htrE"
/locus_tag="b0139"
/gene_synonym="ECK0138; JW0135"
/db_xref="EcoGene:EG11972"
/db_xref="GeneID:944819"
CDS complement(152829..155426)
/gene="htrE"
/locus_tag="b0139"
/gene_synonym="ECK0138; JW0135"
/function="putative membrane; Surface structures"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0009279 - cell outer membrane"
/note="probable outer membrane porin protein involved in
fimbrial assembly"
/codon_start=1
/transl_table=11
/product="putative outer membrane usher protein"
/protein_id="NP_414681.1"
/db_xref="GI:16128132"
/db_xref="ASAP:ABE-0000479"
/db_xref="UniProtKB/Swiss-Prot:P33129"
/db_xref="EcoGene:EG11972"
/db_xref="GeneID:944819"
/translation="MTIEYTKNYHHLTRIATFCALLYCNTAFSAELVEYDHTFLMGQN
ASNIDLSRYSEGNPAIPGVYDVSVYVNDQPIINQSITFVAIEGKKNAQACITLKNLLQ
FHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNDQRLDIDVPQAWVMKN
YQNYVDPSLWENGINAAMLSYNLNGYHSETPGRKNESIYAAFNGGMNLGAWRLRASGN
YNWMTDSGSNYDFKNRYVQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRML
PPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVTI
EESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNNY
LTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWNK
LFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVTV
SINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSTSWGSYSVSAQRSWNE
DGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQISSDFKGNNQLNVSSSGYSDNAR
VSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSISANSDNSRQVSLSTDGGFVL
HSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIA
LDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIY
DEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEQSKPVSCLAHYQQSPEAEKIAQSII
LNGIRCQIQ"
misc_feature complement(152832..155426)
/gene="htrE"
/locus_tag="b0139"
/gene_synonym="ECK0138; JW0135"
/note="putative fimbrial outer membrane usher protein;
Provisional; Region: PRK09828"
/db_xref="CDD:182098"
misc_feature complement(154869..155327)
/gene="htrE"
/locus_tag="b0139"
/gene_synonym="ECK0138; JW0135"
/note="PapC N-terminal domain; Region: PapC_N; pfam13954"
/db_xref="CDD:222472"
misc_feature complement(153114..154832)
/gene="htrE"
/locus_tag="b0139"
/gene_synonym="ECK0138; JW0135"
/note="Type VII secretion system (T7SS), usher protein;
Region: Usher; pfam00577"
/db_xref="CDD:216001"
misc_feature complement(152880..153086)
/gene="htrE"
/locus_tag="b0139"
/gene_synonym="ECK0138; JW0135"
/note="PapC C-terminal domain; Region: PapC_C; pfam13953"
/db_xref="CDD:222471"
gene complement(155461..156201)
/gene="yadV"
/locus_tag="b0140"
/gene_synonym="ECK0139; ecpD; JW0136"
/db_xref="EcoGene:EG11973"
/db_xref="GeneID:944859"
CDS complement(155461..156201)
/gene="yadV"
/locus_tag="b0140"
/gene_synonym="ECK0139; ecpD; JW0136"
/function="putative factor; Surface structures"
/GO_component="GO:0019866 - organelle inner membrane"
/GO_process="GO:0006457 - protein folding"
/note="probable pilin chaperone similar to PapD"
/codon_start=1
/transl_table=11
/product="putative periplasmic pilin chaperone"
/protein_id="NP_414682.1"
/db_xref="GI:16128133"
/db_xref="ASAP:ABE-0000482"
/db_xref="UniProtKB/Swiss-Prot:P33128"
/db_xref="EcoGene:EG11973"
/db_xref="GeneID:944859"
/translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVNG
LNGKANSAKVHFYAINDFGGAIEGNARL"
misc_feature complement(155464..156201)
/gene="yadV"
/locus_tag="b0140"
/gene_synonym="ECK0139; ecpD; JW0136"
/note="putative chaperone protein EcpD; Provisional;
Region: PRK09926"
/db_xref="CDD:236634"
misc_feature complement(155746..156123)
/gene="yadV"
/locus_tag="b0140"
/gene_synonym="ECK0139; ecpD; JW0136"
/note="Gram-negative pili assembly chaperone, N-terminal
domain; Region: Pili_assembly_N; pfam00345"
/db_xref="CDD:215870"
misc_feature complement(155476..155679)
/gene="yadV"
/locus_tag="b0140"
/gene_synonym="ECK0139; ecpD; JW0136"
/note="Gram-negative pili assembly chaperone, C-terminal
domain; Region: Pili_assembly_C; pfam02753"
/db_xref="CDD:217216"
gene complement(156299..156883)
/gene="yadN"
/locus_tag="b0141"
/gene_synonym="ECK0140; JW0137"
/db_xref="EcoGene:EG12328"
/db_xref="GeneID:944841"
CDS complement(156299..156883)
/gene="yadN"
/locus_tag="b0141"
/gene_synonym="ECK0140; JW0137"
/function="putative structure; Not classified"
/GO_component="GO:0009289 - pilus"
/note="putative fimbrial-like protein"
/codon_start=1
/transl_table=11
/product="putative fimbrial-like adhesin protein"
/protein_id="NP_414683.1"
/db_xref="GI:16128134"
/db_xref="ASAP:ABE-0000487"
/db_xref="UniProtKB/Swiss-Prot:P37050"
/db_xref="EcoGene:EG12328"
/db_xref="GeneID:944841"
/translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
misc_feature complement(156302..156859)
/gene="yadN"
/locus_tag="b0141"
/gene_synonym="ECK0140; JW0137"
/note="Fimbrial protein; Region: Fimbrial; cl01416"
/db_xref="CDD:242492"
gene complement(157253..157732)
/gene="folK"
/locus_tag="b0142"
/gene_synonym="ECK0141; JW0138"
/db_xref="EcoGene:EG11374"
/db_xref="GeneID:948792"
CDS complement(157253..157732)
/gene="folK"
/locus_tag="b0142"
/gene_synonym="ECK0141; JW0138"
/EC_number="2.7.6.3"
/function="enzyme; Biosynthesis of cofactors, carriers:
Folic acid"
/GO_process="GO:0046656 - folic acid biosynthetic process"
/note="7,8-dihydro-6-hydroxymethylpterin-
pyrophosphokinase"
/codon_start=1
/transl_table=11
/product="2-amino-4-hydroxy-6-hydroxymethyldihyropteridine
pyrophosphokinase"
/protein_id="NP_414684.1"
/db_xref="GI:16128135"
/db_xref="ASAP:ABE-0000491"
/db_xref="UniProtKB/Swiss-Prot:P26281"
/db_xref="EcoGene:EG11374"
/db_xref="GeneID:948792"
/translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGEMLRQILHTRAFDKLNKW"
mat_peptide complement(157256..157729)
/gene="folK"
/locus_tag="b0142"
/gene_synonym="ECK0141; JW0138"
/product="2-amino-4-hydroxy-6-hydroxymethyldihyropteridine
pyrophosphokinase"
/experiment="N-terminus verified by Edman degradation:
PMID 1325970,1657875"
misc_feature complement(157337..157723)
/gene="folK"
/locus_tag="b0142"
/gene_synonym="ECK0141; JW0138"
/note="7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase
(HPPK). Folate derivatives are essential cofactors in the
biosynthesis of purines, pyrimidines, and amino acids as
well as formyl-tRNA. Mammalian cells are able to utilize
pre-formed folates after...; Region: HPPK; cd00483"
/db_xref="CDD:238269"
misc_feature complement(order(157361..157363,157367..157369,
157382..157387,157394..157396,157436..157441,
157445..157447,157454..157456,157463..157465,
157478..157480,157484..157486,157499..157501,
157508..157510,157520..157522,157565..157567,
157571..157573,157595..157597,157601..157606,
157706..157708))
/gene="folK"
/locus_tag="b0142"
/gene_synonym="ECK0141; JW0138"
/note="catalytic center binding site [active]"
/db_xref="CDD:238269"
misc_feature complement(order(157367..157369,157382..157387,
157394..157396,157436..157441,157454..157456,
157478..157480,157484..157486,157499..157501,
157508..157510,157520..157522))
/gene="folK"
/locus_tag="b0142"
/gene_synonym="ECK0141; JW0138"
/note="ATP binding site [chemical binding]; other site"
/db_xref="CDD:238269"
gene complement(157729..159126)
/gene="pcnB"
/locus_tag="b0143"
/gene_synonym="ECK0142; JW5808"
/db_xref="EcoGene:EG10690"
/db_xref="GeneID:947318"
CDS complement(157729..159126)
/gene="pcnB"
/locus_tag="b0143"
/gene_synonym="ECK0142; JW5808"
/EC_number="2.7.7.19"
/function="enzyme; RNA synthesis, modification, DNA
transcription"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0009451 - RNA modification"
/codon_start=1
/transl_table=11
/product="poly(A) polymerase"
/protein_id="NP_414685.4"
/db_xref="GI:162135892"
/db_xref="ASAP:ABE-0000493"
/db_xref="UniProtKB/Swiss-Prot:P0ABF1"
/db_xref="EcoGene:EG10690"
/db_xref="GeneID:947318"
/translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
TRRPRKRAPRREGTA"
misc_feature complement(157795..159126)
/gene="pcnB"
/locus_tag="b0143"
/gene_synonym="ECK0142; JW5808"
/note="poly(A) polymerase I; Provisional; Region: pcnB;
PRK11623"
/db_xref="CDD:236939"
mat_peptide complement(157732..159096)
/gene="pcnB"
/locus_tag="b0143"
/gene_synonym="ECK0142; JW5808"
/product="poly(A) polymerase"
/experiment="N-terminus verified by Edman degradation:
PMID 1438224"
misc_feature complement(158560..158994)
/gene="pcnB"
/locus_tag="b0143"
/gene_synonym="ECK0142; JW5808"
/note="Nucleotidyltransferase (NT) domain of ClassII
CCA-adding enzymes; Region: NT_ClassII-CCAase; cd05398"
/db_xref="CDD:143388"
misc_feature complement(order(158629..158631,158638..158649,
158755..158757,158770..158772,158809..158811,
158881..158883,158887..158889,158914..158919,
158926..158931))
/gene="pcnB"
/locus_tag="b0143"
/gene_synonym="ECK0142; JW5808"
/note="active site"
/db_xref="CDD:143388"
misc_feature complement(order(158629..158631,158641..158649,
158881..158883,158887..158889,158917..158919,
158926..158931))
/gene="pcnB"
/locus_tag="b0143"
/gene_synonym="ECK0142; JW5808"
/note="NTP binding site [chemical binding]; other site"
/db_xref="CDD:143388"
misc_feature complement(order(158770..158772,158881..158883,
158887..158889))
/gene="pcnB"
/locus_tag="b0143"
/gene_synonym="ECK0142; JW5808"
/note="metal binding triad [ion binding]; metal-binding
site"
/db_xref="CDD:143388"
misc_feature complement(158278..158469)
/gene="pcnB"
/locus_tag="b0143"
/gene_synonym="ECK0142; JW5808"
/note="Probable RNA and SrmB- binding site of polymerase
A; Region: PolyA_pol_RNAbd; pfam12627"
/db_xref="CDD:221674"
misc_feature complement(157795..158124)
/gene="pcnB"
/locus_tag="b0143"
/gene_synonym="ECK0142; JW5808"
/note="Polymerase A arginine-rich C-terminus; Region:
PolyA_pol_arg_C; pfam12626"
/db_xref="CDD:221673"
gene complement(159186..160112)
/gene="gluQ"
/locus_tag="b0144"
/gene_synonym="ECK0143; JW5892; yadB"
/db_xref="EcoGene:EG11362"
/db_xref="GeneID:944846"
CDS complement(159186..160112)
/gene="gluQ"
/locus_tag="b0144"
/gene_synonym="ECK0143; JW5892; yadB"
/EC_number="6.1.1.-"
/function="putative enzyme; Aminoacyl tRNA synthetases,
tRNA modification"
/GO_process="GO:0006418 - tRNA aminoacylation for protein
translation"
/note="putative tRNA synthetase"
/codon_start=1
/transl_table=11
/product="glutamyl-Q tRNA(Asp) synthetase"
/protein_id="NP_414686.3"
/db_xref="GI:226524698"
/db_xref="ASAP:ABE-0000497"
/db_xref="UniProtKB/Swiss-Prot:P27305"
/db_xref="EcoGene:EG11362"
/db_xref="GeneID:944846"
/translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
misc_feature complement(159204..160082)
/gene="gluQ"
/locus_tag="b0144"
/gene_synonym="ECK0143; JW5892; yadB"
/note="glutamyl-Q tRNA(Asp) synthetase; Reviewed; Region:
PRK05710"
/db_xref="CDD:235573"
misc_feature complement(159264..160058)
/gene="gluQ"
/locus_tag="b0144"
/gene_synonym="ECK0143; JW5892; yadB"
/note="tRNA synthetases class I (E and Q), catalytic
domain; Region: tRNA-synt_1c; pfam00749"
/db_xref="CDD:216096"
misc_feature complement(order(159390..159395,160017..160028))
/gene="gluQ"
/locus_tag="b0144"
/gene_synonym="ECK0143; JW5892; yadB"
/note="active site"
/db_xref="CDD:173912"
misc_feature complement(order(159390..159392,160017..160019,
160026..160028))
/gene="gluQ"
/locus_tag="b0144"
/gene_synonym="ECK0143; JW5892; yadB"
/note="nucleotide binding site [chemical binding]; other
site"
/db_xref="CDD:173912"
misc_feature complement(160017..160028)
/gene="gluQ"
/locus_tag="b0144"
/gene_synonym="ECK0143; JW5892; yadB"
/note="HIGH motif; other site"
/db_xref="CDD:173912"
misc_feature complement(159390..159395)
/gene="gluQ"
/locus_tag="b0144"
/gene_synonym="ECK0143; JW5892; yadB"
/note="KMSKS motif; other site"
/db_xref="CDD:173912"
gene complement(160149..160604)
/gene="dksA"
/locus_tag="b0145"
/gene_synonym="ECK0144; JW0141; msmA"
/db_xref="EcoGene:EG10230"
/db_xref="GeneID:944850"
CDS complement(160149..160604)
/gene="dksA"
/locus_tag="b0145"
/gene_synonym="ECK0144; JW0141; msmA"
/function="phenotype; DNA - replication, repair,
restriction/modification"
/experiment="N-terminus verified by Edman degradation:
PMID 9298646,9600841,9740056"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006457 - protein folding"
/note="dnaK suppressor protein"
/codon_start=1
/transl_table=11
/product="DNA-binding transcriptional regulator of rRNA
transcription, DnaK suppressor protein"
/protein_id="NP_414687.1"
/db_xref="GI:16128138"
/db_xref="ASAP:ABE-0000499"
/db_xref="UniProtKB/Swiss-Prot:P0ABS1"
/db_xref="EcoGene:EG10230"
/db_xref="GeneID:944850"
/translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
misc_feature complement(160152..160604)
/gene="dksA"
/locus_tag="b0145"
/gene_synonym="ECK0144; JW0141; msmA"
/note="RNA polymerase-binding transcription factor;
Provisional; Region: dksA; PRK10778"
/db_xref="CDD:182722"
gene complement(160782..161486)
/gene="sfsA"
/locus_tag="b0146"
/gene_synonym="ECK0145; JW0142; sfs; sfs1"
/db_xref="EcoGene:EG10949"
/db_xref="GeneID:944855"
CDS complement(160782..161486)
/gene="sfsA"
/locus_tag="b0146"
/gene_synonym="ECK0145; JW0142; sfs; sfs1"
/function="putative regulator; Degradation of small
molecules: Carbon compounds"
/GO_process="GO:0016052 - carbohydrate catabolic process"
/note="probable regulator for maltose metabolism"
/codon_start=1
/transl_table=11
/product="putative DNA-binding transcriptional regulator"
/protein_id="NP_414688.1"
/db_xref="GI:16128139"
/db_xref="ASAP:ABE-0000503"
/db_xref="UniProtKB/Swiss-Prot:P0A823"
/db_xref="EcoGene:EG10949"
/db_xref="GeneID:944855"
/translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
ISAEGMALKKSLPVTL"
misc_feature complement(160785..161486)
/gene="sfsA"
/locus_tag="b0146"
/gene_synonym="ECK0145; JW0142; sfs; sfs1"
/note="putative DNA-binding transcriptional regulator;
Reviewed; Region: PRK00347"
/db_xref="CDD:234733"
gene complement(161501..162031)
/gene="ligT"
/locus_tag="b0147"
/gene_synonym="ECK0146; JW5011; yadP"
/db_xref="EcoGene:EG12330"
/db_xref="GeneID:944848"
CDS complement(161501..162031)
/gene="ligT"
/locus_tag="b0147"
/gene_synonym="ECK0146; JW5011; yadP"
/EC_number="6.5.1.-"
/function="enzyme; Unclassified"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0009451 - RNA modification"
/codon_start=1
/transl_table=11
/product="2'-5' RNA ligase"
/protein_id="NP_414689.4"
/db_xref="GI:90111091"
/db_xref="ASAP:ABE-0000505"
/db_xref="UniProtKB/Swiss-Prot:P37025"
/db_xref="EcoGene:EG12330"
/db_xref="GeneID:944848"
/translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
RGRTRYTPLKRWALTQ"
misc_feature complement(161504..162031)
/gene="ligT"
/locus_tag="b0147"
/gene_synonym="ECK0146; JW5011; yadP"
/note="2'-5' RNA ligase; Provisional; Region: PRK15124"
/db_xref="CDD:185079"
mat_peptide complement(161504..162028)
/gene="ligT"
/locus_tag="b0147"
/gene_synonym="ECK0146; JW5011; yadP"
/product="2'-5' RNA ligase"
/experiment="N-terminus verified by Edman degradation:
PMID 8940112"
misc_feature complement(161765..162001)
/gene="ligT"
/locus_tag="b0147"
/gene_synonym="ECK0146; JW5011; yadP"
/note="LigT like Phosphoesterase; Region: LigT_PEase;
pfam02834"
/db_xref="CDD:217246"
misc_feature complement(161534..161758)
/gene="ligT"
/locus_tag="b0147"
/gene_synonym="ECK0146; JW5011; yadP"
/note="LigT like Phosphoesterase; Region: LigT_PEase;
pfam02834"
/db_xref="CDD:217246"
gene 162105..164534
/gene="hrpB"
/locus_tag="b0148"
/gene_synonym="ECK0147; JW0144; yadO"
/db_xref="EcoGene:EG12329"
/db_xref="GeneID:944845"
CDS 162105..164534
/gene="hrpB"
/locus_tag="b0148"
/gene_synonym="ECK0147; JW0144; yadO"
/function="enzyme; DNA - replication, repair,
restriction/modification"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006261 - DNA-dependent DNA replication"
/note="helicase, ATP-dependent"
/codon_start=1
/transl_table=11
/product="putative ATP-dependent helicase"
/protein_id="NP_414690.4"
/db_xref="GI:162135893"
/db_xref="ASAP:ABE-0000510"
/db_xref="UniProtKB/Swiss-Prot:P37024"
/db_xref="EcoGene:EG12329"
/db_xref="GeneID:944845"
/translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALLVDIDELVQRCPQL
VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
PTIAQGRVPLVLELLSPAQRPLQITRDLSDFWKGAYREVQKEMKGRYPKHVWPDDPAN
TAPTRRTKKYS"
misc_feature 162105..164531
/gene="hrpB"
/locus_tag="b0148"
/gene_synonym="ECK0147; JW0144; yadO"
/note="ATP-dependent RNA helicase HrpB; Provisional;
Region: PRK11664"
/db_xref="CDD:236950"
misc_feature 162171..162581
/gene="hrpB"
/locus_tag="b0148"
/gene_synonym="ECK0147; JW0144; yadO"
/note="DEAD-like helicases superfamily. A diverse family
of proteins involved in ATP-dependent RNA or DNA
unwinding. This domain contains the ATP-binding region;
Region: DEXDc; cd00046"
/db_xref="CDD:238005"
misc_feature 162192..162206
/gene="hrpB"
/locus_tag="b0148"
/gene_synonym="ECK0147; JW0144; yadO"
/note="ATP binding site [chemical binding]; other site"
/db_xref="CDD:238005"
misc_feature 162471..162482
/gene="hrpB"
/locus_tag="b0148"
/gene_synonym="ECK0147; JW0144; yadO"
/note="putative Mg++ binding site [ion binding]; other
site"
/db_xref="CDD:238005"
misc_feature 162720..163109
/gene="hrpB"
/locus_tag="b0148"
/gene_synonym="ECK0147; JW0144; yadO"
/note="Helicase superfamily c-terminal domain; associated
with DEXDc-, DEAD-, and DEAH-box proteins, yeast
initiation factor 4A, Ski2p, and Hepatitis C virus NS3
helicases; this domain is found in a wide variety of
helicases and helicase related proteins; may...; Region:
HELICc; cd00079"
/db_xref="CDD:238034"
misc_feature order(162762..162773,162840..162845,162918..162926)
/gene="hrpB"
/locus_tag="b0148"
/gene_synonym="ECK0147; JW0144; yadO"
/note="nucleotide binding region [chemical binding]; other
site"
/db_xref="CDD:238034"
misc_feature order(162942..162944,163059..163061,163071..163073,
163080..163082)
/gene="hrpB"
/locus_tag="b0148"
/gene_synonym="ECK0147; JW0144; yadO"
/note="ATP-binding site [chemical binding]; other site"
/db_xref="CDD:238034"
misc_feature 163287..>163388
/gene="hrpB"
/locus_tag="b0148"
/gene_synonym="ECK0147; JW0144; yadO"
/note="Helicase associated domain (HA2) Add an annotation;
Region: HA2; smart00847"
/db_xref="CDD:214852"
misc_feature 164094..164492
/gene="hrpB"
/locus_tag="b0148"
/gene_synonym="ECK0147; JW0144; yadO"
/note="ATP-dependent helicase C-terminal; Region: HrpB_C;
pfam08482"
/db_xref="CDD:149509"
gene 164730..167264
/gene="mrcB"
/locus_tag="b0149"
/gene_synonym="ECK0148; JW0145; pbpF; ponB"
/db_xref="EcoGene:EG10605"
/db_xref="GeneID:944843"
CDS 164730..167264
/gene="mrcB"
/locus_tag="b0149"
/gene_synonym="ECK0148; JW0145; pbpF; ponB"
/EC_number="2.4.1.129"
/EC_number="3.4.-.-"
/function="enzyme; Murein sacculus, peptidoglycan"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/GO_process="GO:0046349 - amino sugar biosynthetic
process"
/GO_process="GO:0009252 - peptidoglycan biosynthetic
process"
/GO_process="GO:0042493 - response to drug"
/note="peptidoglycan synthetase; penicillin-binding
protein 1B"
/codon_start=1
/transl_table=11
/product="fused glycosyl transferase and transpeptidase"
/protein_id="NP_414691.1"
/db_xref="GI:16128142"
/db_xref="ASAP:ABE-0000516"
/db_xref="UniProtKB/Swiss-Prot:P02919"
/db_xref="EcoGene:EG10605"
/db_xref="GeneID:944843"
/translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRILPVWTSDPQSLCQQSE
MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
misc_feature 164730..167258
/gene="mrcB"
/locus_tag="b0149"
/gene_synonym="ECK0148; JW0145; pbpF; ponB"
/note="bifunctional glycosyl transferase/transpeptidase;
Reviewed; Region: mrcB; PRK09506"
/db_xref="CDD:236544"
misc_feature 165336..165830
/gene="mrcB"
/locus_tag="b0149"
/gene_synonym="ECK0148; JW0145; pbpF; ponB"
/note="Transglycosylase; Region: Transgly; pfam00912"
/db_xref="CDD:201501"
misc_feature 166146..166871
/gene="mrcB"
/locus_tag="b0149"
/gene_synonym="ECK0148; JW0145; pbpF; ponB"
/note="Penicillin binding protein transpeptidase domain;
Region: Transpeptidase; cl17760"
/db_xref="CDD:248314"
repeat_region 167281..167305
/note="REP12 (repetitive extragenic palindromic) element;
contains 1 REP sequences"
gene 167484..169727
/gene="fhuA"
/locus_tag="b0150"
/gene_synonym="ECK0149; JW0146; T1; tonA"
/db_xref="EcoGene:EG10302"
/db_xref="GeneID:944856"
CDS 167484..169727
/gene="fhuA"
/locus_tag="b0150"
/gene_synonym="ECK0149; JW0146; T1; tonA"
/function="membrane; Outer membrane constituents"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0009279 - cell outer membrane"
/note="outer membrane protein receptor for ferrichrome,
colicin M, and phages T1, T5, and phi80"
/codon_start=1
/transl_table=11
/product="ferrichrome outer membrane transporter"
/protein_id="NP_414692.1"
/db_xref="GI:16128143"
/db_xref="ASAP:ABE-0000520"
/db_xref="UniProtKB/Swiss-Prot:P06971"
/db_xref="EcoGene:EG10302"
/db_xref="GeneID:944856"
/translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
ATATFRF"
misc_feature 167484..169724
/gene="fhuA"
/locus_tag="b0150"
/gene_synonym="ECK0149; JW0146; T1; tonA"
/note="ferrichrome outer membrane transporter;
Provisional; Region: PRK10044"
/db_xref="CDD:236643"
mat_peptide 167583..169724
/gene="fhuA"
/locus_tag="b0150"
/gene_synonym="ECK0149; JW0146; T1; tonA"
/product="ferrichrome outer membrane transporter"
/experiment="N-terminus verified by Edman degradation:
PMID 3079747"
misc_feature 167727..169724
/gene="fhuA"
/locus_tag="b0150"
/gene_synonym="ECK0149; JW0146; T1; tonA"
/note="TonB dependent/Ligand-Gated channels are created by
a monomeric 22 strand (22,24) anti-parallel beta-barrel.
Ligands apparently bind to the large extracellular loops.
The N-terminal 150-200 residues form a plug from the
periplasmic end of barrel; Region: ligand_gated_channel;
cd01347"
/db_xref="CDD:238657"
misc_feature order(167727..167756,167784..167813,167850..167867,
167886..167888,167895..167915,167949..167981,
168015..168041)
/gene="fhuA"
/locus_tag="b0150"
/gene_synonym="ECK0149; JW0146; T1; tonA"
/note="N-terminal plug; other site"
/db_xref="CDD:238657"
misc_feature order(168501..168503,168630..168632)
/gene="fhuA"
/locus_tag="b0150"
/gene_synonym="ECK0149; JW0146; T1; tonA"
/note="ligand-binding site [chemical binding]; other site"
/db_xref="CDD:238657"
gene 169778..170575
/gene="fhuC"
/locus_tag="b0151"
/gene_synonym="ECK0150; JW0147"
/db_xref="EcoGene:EG10304"
/db_xref="GeneID:945250"
CDS 169778..170575
/gene="fhuC"
/locus_tag="b0151"
/gene_synonym="ECK0150; JW0147"
/function="transport; Transport of small molecules:
Cations"
/GO_component="GO:0005737 - cytoplasm"
/note="ATP-binding component of hydroxymate-dependent iron
transport"
/codon_start=1
/transl_table=11
/product="iron-hydroxamate transporter subunit"
/protein_id="NP_414693.1"
/db_xref="GI:16128144"
/db_xref="ASAP:ABE-0000522"
/db_xref="UniProtKB/Swiss-Prot:P07821"
/db_xref="EcoGene:EG10304"
/db_xref="GeneID:945250"
/translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
LVAQDSRCLLLDEPTSALDIAHQVDVLSLVHRLSQERGLTVIAVLHDINMAARYCDYL
VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
misc_feature 169778..170572
/gene="fhuC"
/locus_tag="b0151"
/gene_synonym="ECK0150; JW0147"
/note="iron-hydroxamate transporter ATP-binding subunit;
Provisional; Region: PRK10575"
/db_xref="CDD:182561"
misc_feature 169814..170467
/gene="fhuC"
/locus_tag="b0151"
/gene_synonym="ECK0150; JW0147"
/note="ATP-binding component of iron-siderophores, vitamin
B12 and hemin transporters and related proteins; Region:
ABC_Iron-Siderophores_B12_Hemin; cd03214"
/db_xref="CDD:213181"
misc_feature 169907..169930
/gene="fhuC"
/locus_tag="b0151"
/gene_synonym="ECK0150; JW0147"
/note="Walker A/P-loop; other site"
/db_xref="CDD:213181"
misc_feature order(169916..169921,169925..169933,170051..170053,
170291..170296,170393..170395)
/gene="fhuC"
/locus_tag="b0151"
/gene_synonym="ECK0150; JW0147"
/note="ATP binding site [chemical binding]; other site"
/db_xref="CDD:213181"
misc_feature 170042..170053
/gene="fhuC"
/locus_tag="b0151"
/gene_synonym="ECK0150; JW0147"
/note="Q-loop/lid; other site"
/db_xref="CDD:213181"
misc_feature 170219..170248
/gene="fhuC"
/locus_tag="b0151"
/gene_synonym="ECK0150; JW0147"
/note="ABC transporter signature motif; other site"
/db_xref="CDD:213181"
misc_feature 170279..170296
/gene="fhuC"
/locus_tag="b0151"
/gene_synonym="ECK0150; JW0147"
/note="Walker B; other site"
/db_xref="CDD:213181"
misc_feature 170303..170314
/gene="fhuC"
/locus_tag="b0151"
/gene_synonym="ECK0150; JW0147"
/note="D-loop; other site"
/db_xref="CDD:213181"
misc_feature 170381..170401
/gene="fhuC"
/locus_tag="b0151"
/gene_synonym="ECK0150; JW0147"
/note="H-loop/switch region; other site"
/db_xref="CDD:213181"
gene 170575..171465
/gene="fhuD"
/locus_tag="b0152"
/gene_synonym="ECK0151; JW0148"
/db_xref="EcoGene:EG10305"
/db_xref="GeneID:947510"
CDS 170575..171465
/gene="fhuD"
/locus_tag="b0152"
/gene_synonym="ECK0151; JW0148"
/function="transport; Transport of small molecules:
Cations"
/GO_component="GO:0042597 - periplasmic space"
/GO_process="GO:0006826 - iron ion transport"
/note="hydroxamate-dependent iron uptake, cytoplasmic
membrane component; periplasmic-binding component of ABC
superfamily"
/codon_start=1
/transl_table=11
/product="iron-hydroxamate transporter subunit"
/protein_id="NP_414694.1"
/db_xref="GI:16128145"
/db_xref="ASAP:ABE-0000524"
/db_xref="UniProtKB/Swiss-Prot:P07822"
/db_xref="EcoGene:EG10305"
/db_xref="GeneID:947510"
/translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
ATLSAMHFVRVLDNAIGGKA"
misc_feature 170674..171429
/gene="fhuD"
/locus_tag="b0152"
/gene_synonym="ECK0151; JW0148"
/note="Fe3+-siderophore binding domain FhuD. These
proteins have been shown to function as initial receptors
in ABC transport of Fe3+-siderophores in many eubacterial
species. They belong to the TroA-like superfamily of
helical backbone metal receptor proteins...; Region: FhuD;
cd01146"
/db_xref="CDD:238566"
misc_feature 170731..171393
/gene="fhuD"
/locus_tag="b0152"
/gene_synonym="ECK0151; JW0148"
/note="Periplasmic binding protein; Region: Peripla_BP_2;
pfam01497"
/db_xref="CDD:216532"
misc_feature order(170776..170778,170824..170829,170890..170892,
171139..171141)
/gene="fhuD"
/locus_tag="b0152"
/gene_synonym="ECK0151; JW0148"
/note="siderophore binding site; other site"
/db_xref="CDD:238566"
gene 171462..173444
/gene="fhuB"
/locus_tag="b0153"
/gene_synonym="ECK0152; JW0149"
/db_xref="EcoGene:EG10303"
/db_xref="GeneID:946890"
CDS 171462..173444
/gene="fhuB"
/locus_tag="b0153"
/gene_synonym="ECK0152; JW0149"
/function="transport; Transport of small molecules:
Cations"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/codon_start=1
/transl_table=11
/product="fused iron-hydroxamate transporter subunits of
ABC superfamily: membrane components"
/protein_id="NP_414695.1"
/db_xref="GI:16128146"
/db_xref="ASAP:ABE-0000526"
/db_xref="UniProtKB/Swiss-Prot:P06972"
/db_xref="EcoGene:EG10303"
/db_xref="GeneID:946890"
/translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
ITVTTLWAIPGAMASQFAAQAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
misc_feature 171534..173441
/gene="fhuB"
/locus_tag="b0153"
/gene_synonym="ECK0152; JW0149"
/note="iron-hydroxamate transporter permease subunit;
Provisional; Region: PRK10577"
/db_xref="CDD:182563"
misc_feature 171711..172394
/gene="fhuB"
/locus_tag="b0153"
/gene_synonym="ECK0152; JW0149"
/note="Transmembrane subunit (TM), of Periplasmic Binding
Protein (PBP)-dependent ATP-Binding Cassette (ABC)
transporters involved in the uptake of siderophores, heme,
vitamin B12, or the divalent cations Mg2+ and Zn2+.
PBP-dependent ABC transporters consist of...; Region:
TM_ABC_iron-siderophores_like; cd06550"
/db_xref="CDD:119348"
misc_feature order(171711..171719,172077..172082,172086..172094,
172098..172103,172107..172124,172128..172136,
172257..172259,172278..172280)
/gene="fhuB"
/locus_tag="b0153"
/gene_synonym="ECK0152; JW0149"
/note="ABC-ATPase subunit interface; other site"
/db_xref="CDD:119348"
misc_feature order(171714..171716,171720..171722,171735..171737,
171894..171896,171900..171905,171912..171917,
171924..171929,171936..171938,171945..171950,
171954..171956,171987..171992,171999..172001,
172227..172229,172386..172388)
/gene="fhuB"
/locus_tag="b0153"
/gene_synonym="ECK0152; JW0149"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:119348"
misc_feature order(171954..171956,172029..172031,172203..172205,
172215..172217,172347..172349,172377..172379)
/gene="fhuB"
/locus_tag="b0153"
/gene_synonym="ECK0152; JW0149"
/note="putative PBP binding regions; other site"
/db_xref="CDD:119348"
misc_feature 172641..173426
/gene="fhuB"
/locus_tag="b0153"
/gene_synonym="ECK0152; JW0149"
/note="Transmembrane subunit (TM), of Periplasmic Binding
Protein (PBP)-dependent ATP-Binding Cassette (ABC)
transporters involved in the uptake of siderophores, heme,
vitamin B12, or the divalent cations Mg2+ and Zn2+.
PBP-dependent ABC transporters consist of...; Region:
TM_ABC_iron-siderophores_like; cd06550"
/db_xref="CDD:119348"
misc_feature order(172695..172697,172707..172715,173076..173081,
173085..173093,173097..173102,173106..173123,
173127..173135,173256..173258,173277..173279)
/gene="fhuB"
/locus_tag="b0153"
/gene_synonym="ECK0152; JW0149"
/note="ABC-ATPase subunit interface; other site"
/db_xref="CDD:119348"
misc_feature order(172710..172712,172716..172718,172731..172733,
172893..172895,172899..172904,172911..172916,
172923..172928,172935..172937,172944..172949,
172953..172955,172986..172991,172998..173000,
173226..173228,173382..173384,173391..173396,
173403..173405,173412..173417,173424..173426)
/gene="fhuB"
/locus_tag="b0153"
/gene_synonym="ECK0152; JW0149"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:119348"
misc_feature order(172953..172955,173028..173030,173202..173204,
173214..173216,173346..173348,173373..173375)
/gene="fhuB"
/locus_tag="b0153"
/gene_synonym="ECK0152; JW0149"
/note="putative PBP binding regions; other site"
/db_xref="CDD:119348"
repeat_region 173504..173588
/note="REP13 (repetitive extragenic palindromic) element;
contains 2 REP sequences"
gene complement(173602..174882)
/gene="hemL"
/locus_tag="b0154"
/gene_synonym="ECK0153; gsa?; JW0150; popC"
/db_xref="EcoGene:EG10432"
/db_xref="GeneID:946892"
CDS complement(173602..174882)
/gene="hemL"
/locus_tag="b0154"
/gene_synonym="ECK0153; gsa?; JW0150; popC"
/EC_number="5.4.3.8"
/function="enzyme; Biosynthesis of cofactors, carriers:
Heme, porphyrin"
/codon_start=1
/transl_table=11
/product="glutamate-1-semialdehyde aminotransferase
(aminomutase)"
/protein_id="NP_414696.1"
/db_xref="GI:16128147"
/db_xref="ASAP:ABE-0000529"
/db_xref="UniProtKB/Swiss-Prot:P23893"
/db_xref="EcoGene:EG10432"
/db_xref="GeneID:946892"
/translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
GQPNSPGVPADFAKYTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
misc_feature complement(173605..174882)
/gene="hemL"
/locus_tag="b0154"
/gene_synonym="ECK0153; gsa?; JW0150; popC"
/note="glutamate-1-semialdehyde aminotransferase;
Provisional; Region: PRK00062"
/db_xref="CDD:234607"
misc_feature complement(173614..174864)
/gene="hemL"
/locus_tag="b0154"
/gene_synonym="ECK0153; gsa?; JW0150; popC"
/note="Acetyl ornithine aminotransferase family. This
family belongs to pyridoxal phosphate (PLP)-dependent
aspartate aminotransferase superfamily (fold I). The major
groups in this CD correspond to ornithine
aminotransferase, acetylornithine aminotransferase;
Region: OAT_like; cd00610"
/db_xref="CDD:99735"
misc_feature complement(order(174088..174090,174163..174168,
174172..174174,174271..174273,174448..174450,
174454..174459,174535..174543))
/gene="hemL"
/locus_tag="b0154"
/gene_synonym="ECK0153; gsa?; JW0150; popC"
/note="inhibitor-cofactor binding pocket; inhibition site"
/db_xref="CDD:99735"
misc_feature complement(order(174088..174090,174163..174165,
174172..174174,174271..174273,174454..174459,
174535..174540))
/gene="hemL"
/locus_tag="b0154"
/gene_synonym="ECK0153; gsa?; JW0150; popC"
/note="pyridoxal 5'-phosphate binding site [chemical
binding]; other site"
/db_xref="CDD:99735"
misc_feature complement(174088..174090)
/gene="hemL"
/locus_tag="b0154"
/gene_synonym="ECK0153; gsa?; JW0150; popC"
/note="catalytic residue [active]"
/db_xref="CDD:99735"
gene 175107..176528
/gene="clcA"
/locus_tag="b0155"
/gene_synonym="ECK0154; eriC; JW5012; yadQ"
/db_xref="EcoGene:EG12331"
/db_xref="GeneID:946715"
CDS 175107..176528
/gene="clcA"
/locus_tag="b0155"
/gene_synonym="ECK0154; eriC; JW5012; yadQ"
/function="transport; Not classified"
/note="putative channel transporter"
/codon_start=1
/transl_table=11
/product="H(+)/Cl(-) exchange transporter"
/protein_id="NP_414697.1"
/db_xref="GI:16128148"
/db_xref="ASAP:ABE-0000533"
/db_xref="UniProtKB/Swiss-Prot:P37019"
/db_xref="EcoGene:EG12331"
/db_xref="GeneID:946715"
/translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
RTLAKQEAEQLARSKAASASENT"
misc_feature 175263..176495
/gene="clcA"
/locus_tag="b0155"
/gene_synonym="ECK0154; eriC; JW5012; yadQ"
/note="Chloride channel protein EriC [Inorganic ion
transport and metabolism]; Region: EriC; COG0038"
/db_xref="CDD:223116"
misc_feature 175263..176456
/gene="clcA"
/locus_tag="b0155"
/gene_synonym="ECK0154; eriC; JW5012; yadQ"
/note="ClC chloride channel EriC. This domain is found in
the EriC chloride transporters that mediate the extreme
acid resistance response in eubacteria and archaea. This
response allows bacteria to survive in the acidic
environments by decarboxylation-linked...; Region: EriC;
cd01031"
/db_xref="CDD:238504"
misc_feature order(175422..175436,175542..175556,176169..176183,
176439..176441)
/gene="clcA"
/locus_tag="b0155"
/gene_synonym="ECK0154; eriC; JW5012; yadQ"
/note="Cl- selectivity filter; other site"
/db_xref="CDD:238504"
misc_feature order(175425..175427,175431..175433,175548..175553,
176169..176177,176439..176441)
/gene="clcA"
/locus_tag="b0155"
/gene_synonym="ECK0154; eriC; JW5012; yadQ"
/note="Cl- binding residues [ion binding]; other site"
/db_xref="CDD:238504"
misc_feature 175548..175550
/gene="clcA"
/locus_tag="b0155"
/gene_synonym="ECK0154; eriC; JW5012; yadQ"
/note="pore gating glutamate residue; other site"
/db_xref="CDD:238504"
misc_feature order(175686..175688,175713..175715,175752..175754,
175773..175775,176313..176315,176322..176324,
176346..176348,176382..176384,176403..176408,
176415..176417)
/gene="clcA"
/locus_tag="b0155"
/gene_synonym="ECK0154; eriC; JW5012; yadQ"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:238504"
misc_feature 175713..175715
/gene="clcA"
/locus_tag="b0155"
/gene_synonym="ECK0154; eriC; JW5012; yadQ"
/note="H+/Cl- coupling transport residue; other site"
/db_xref="CDD:238504"
gene 176610..176954
/gene="erpA"
/locus_tag="b0156"
/gene_synonym="ECK0155; JW0152; yadR"
/db_xref="EcoGene:EG12332"
/db_xref="GeneID:944857"
CDS 176610..176954
/gene="erpA"
/locus_tag="b0156"
/gene_synonym="ECK0155; JW0152; yadR"
/codon_start=1
/transl_table=11
/product="iron-sulfur cluster insertion protein"
/protein_id="NP_414698.1"
/db_xref="GI:16128149"
/db_xref="ASAP:ABE-0000538"
/db_xref="UniProtKB/Swiss-Prot:P0ACC3"
/db_xref="EcoGene:EG12332"
/db_xref="GeneID:944857"
/translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
KSTCGCGSSFSI"
misc_feature 176610..176951
/gene="erpA"
/locus_tag="b0156"
/gene_synonym="ECK0155; JW0152; yadR"
/note="iron-sulfur cluster insertion protein ErpA;
Provisional; Region: PRK13623"
/db_xref="CDD:184186"
repeat_region 176969..176987
/note="REP14 (repetitive extragenic palindromic) element;
contains 1 REP sequences"
gene complement(177001..177624)
/gene="yadS"
/locus_tag="b0157"
/gene_synonym="ECK0156; JW0153"
/db_xref="EcoGene:EG12333"
/db_xref="GeneID:949062"
CDS complement(177001..177624)
/gene="yadS"
/locus_tag="b0157"
/gene_synonym="ECK0156; JW0153"
/codon_start=1
/transl_table=11
/product="inner membrane protein, UPF0126 family"
/protein_id="NP_414699.1"
/db_xref="GI:16128150"
/db_xref="ASAP:ABE-0000540"
/db_xref="UniProtKB/Swiss-Prot:P0AFP0"
/db_xref="EcoGene:EG12333"
/db_xref="GeneID:949062"
/translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
misc_feature complement(177004..177624)
/gene="yadS"
/locus_tag="b0157"
/gene_synonym="ECK0156; JW0153"
/note="hypothetical protein; Provisional; Region:
PRK10578"
/db_xref="CDD:182564"
misc_feature complement(177385..177618)
/gene="yadS"
/locus_tag="b0157"
/gene_synonym="ECK0156; JW0153"
/note="UPF0126 domain; Region: UPF0126; pfam03458"
/db_xref="CDD:217571"
misc_feature complement(177145..177357)
/gene="yadS"
/locus_tag="b0157"
/gene_synonym="ECK0156; JW0153"
/note="UPF0126 domain; Region: UPF0126; pfam03458"
/db_xref="CDD:217571"
gene complement(177662..178462)
/gene="btuF"
/locus_tag="b0158"
/gene_synonym="ECK0157; JW0154; yadT"
/db_xref="EcoGene:EG12334"
/db_xref="GeneID:947574"
CDS complement(177662..178462)
/gene="btuF"
/locus_tag="b0158"
/gene_synonym="ECK0157; JW0154; yadT"
/GO_component="GO:0042597 - periplasmic space"
/codon_start=1
/transl_table=11
/product="vitamin B12 transporter subunit:
periplasmic-binding component of ABC superfamily"
/protein_id="NP_414700.1"
/db_xref="GI:16128151"
/db_xref="ASAP:ABE-0000542"
/db_xref="UniProtKB/Swiss-Prot:P37028"
/db_xref="EcoGene:EG12334"
/db_xref="GeneID:947574"
/translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
misc_feature complement(177665..178444)
/gene="btuF"
/locus_tag="b0158"
/gene_synonym="ECK0157; JW0154; yadT"
/note="vitamin B12-transporter protein BtuF; Provisional;
Region: PRK03379"
/db_xref="CDD:179575"
mat_peptide complement(177665..178396)
/gene="btuF"
/locus_tag="b0158"
/gene_synonym="ECK0157; JW0154; yadT"
/product="vitamin B12 transporter subunit:
periplasmic-binding component of ABC superfamily"
/experiment="N-terminus verified by Edman degradation:
PMID 11790740"
misc_feature complement(177683..178390)
/gene="btuF"
/locus_tag="b0158"
/gene_synonym="ECK0157; JW0154; yadT"
/note="Cobalamin binding protein BtuF. These proteins
have been shown to function as initial receptors in ABC
transport of vitamin B12 (cobalamin) in eubacterial and
some archaeal species. They belong to the TroA
superfamily of helical backbone metal receptor...; Region:
BtuF; cd01144"
/db_xref="CDD:238564"
misc_feature complement(order(177875..177877,177959..177961,
178202..178204,178208..178210,178313..178315,
178367..178372))
/gene="btuF"
/locus_tag="b0158"
/gene_synonym="ECK0157; JW0154; yadT"
/note="cobalamin binding residues [chemical binding];
other site"
/db_xref="CDD:238564"
misc_feature complement(order(177857..177859,178247..178249))
/gene="btuF"
/locus_tag="b0158"
/gene_synonym="ECK0157; JW0154; yadT"
/note="putative BtuC binding residues; other site"
/db_xref="CDD:238564"
misc_feature complement(order(178100..178105,178112..178114,
178121..178126,178130..178132,178139..178141,
178151..178153,178169..178171,178178..178180,
178205..178207))
/gene="btuF"
/locus_tag="b0158"
/gene_synonym="ECK0157; JW0154; yadT"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:238564"
gene complement(178455..179153)
/gene="mtn"
/locus_tag="b0159"
/gene_synonym="ECK0158; JW0155; mtnN; pfs; yadA"
/db_xref="EcoGene:EG11090"
/db_xref="GeneID:948542"
CDS complement(178455..179153)
/gene="mtn"
/locus_tag="b0159"
/gene_synonym="ECK0158; JW0155; mtnN; pfs; yadA"
/EC_number="3.2.2.16"
/EC_number="3.2.2.9"
/GO_process="GO:0015949 - nucleobase, nucleoside and
nucleotide interconversion"
/codon_start=1
/transl_table=11
/product="5'-methylthioadenosine/S-adenosylhomocysteine
nucleosidase"
/protein_id="NP_414701.1"
/db_xref="GI:16128152"
/db_xref="ASAP:ABE-0000545"
/db_xref="UniProtKB/Swiss-Prot:P0AF12"
/db_xref="EcoGene:EG11090"
/db_xref="GeneID:948542"
/translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
SLMVESLVQKLAHG"
misc_feature complement(178464..179153)
/gene="mtn"
/locus_tag="b0159"
/gene_synonym="ECK0158; JW0155; mtnN; pfs; yadA"
/note="5'-methylthioadenosine/S-adenosylhomocysteine
nucleosidase; Validated; Region: PRK05584"
/db_xref="CDD:180148"
misc_feature complement(178467..179150)
/gene="mtn"
/locus_tag="b0159"
/gene_synonym="ECK0158; JW0155; mtnN; pfs; yadA"
/note="5'-methylthioadenosine/S-adenosylhomocysteine
nucleosidase; Region: MTA/SAH-Nsdase; TIGR01704"
/db_xref="CDD:130765"
gene 179237..180754
/gene="dgt"
/locus_tag="b0160"
/gene_synonym="ECK0159; JW0156; optA"
/db_xref="EcoGene:EG10225"
/db_xref="GeneID:947177"
CDS 179237..180754
/gene="dgt"
/locus_tag="b0160"
/gene_synonym="ECK0159; JW0156; optA"
/EC_number="3.1.5.1"
/function="enzyme; Central intermediary metabolism:
Nucleotide hydrolysis"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0015949 - nucleobase, nucleoside and
nucleotide interconversion"
/codon_start=1
/transl_table=11
/product="deoxyguanosine triphosphate triphosphohydrolase"
/protein_id="NP_414702.1"
/db_xref="GI:16128153"
/db_xref="ASAP:ABE-0000548"
/db_xref="UniProtKB/Swiss-Prot:P15723"
/db_xref="EcoGene:EG10225"
/db_xref="GeneID:947177"
/translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
HPDVERLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
misc_feature 179237..180748
/gene="dgt"
/locus_tag="b0160"
/gene_synonym="ECK0159; JW0156; optA"
/note="deoxyguanosinetriphosphate triphosphohydrolase;
Provisional; Region: dgt; PRK04926"
/db_xref="CDD:235320"
mat_peptide 179240..180751
/gene="dgt"
/locus_tag="b0160"
/gene_synonym="ECK0159; JW0156; optA"
/product="deoxyguanosine triphosphate triphosphohydrolase"
/experiment="N-terminus verified by Edman degradation:
PMID 2826481,2833745"
misc_feature 179426..>179674
/gene="dgt"
/locus_tag="b0160"
/gene_synonym="ECK0159; JW0156; optA"
/note="Metal dependent phosphohydrolases with conserved
'HD' motif; Region: HDc; cd00077"
/db_xref="CDD:238032"
misc_feature order(179441..179443,179585..179590)
/gene="dgt"
/locus_tag="b0160"
/gene_synonym="ECK0159; JW0156; optA"
/note="Zn2+ binding site [ion binding]; other site"
/db_xref="CDD:238032"
misc_feature 179588..179590
/gene="dgt"
/locus_tag="b0160"
/gene_synonym="ECK0159; JW0156; optA"
/note="Mg2+ binding site [ion binding]; other site"
/db_xref="CDD:238032"
misc_feature 180371..180733
/gene="dgt"
/locus_tag="b0160"
/gene_synonym="ECK0159; JW0156; optA"
/note="Phosphohydrolase-associated domain; Region:
HD_assoc; pfam13286"
/db_xref="CDD:222025"
gene 180884..182308
/gene="degP"
/locus_tag="b0161"
/gene_synonym="ECK0160; htrA; JW0157; ptd"
/db_xref="EcoGene:EG10463"
/db_xref="GeneID:947139"
CDS 180884..182308
/gene="degP"
/locus_tag="b0161"
/gene_synonym="ECK0160; htrA; JW0157; ptd"
/EC_number="3.4.21.-"
/function="enzyme; Degradation of proteins, peptides,
glycopeptides"
/GO_component="GO:0042597 - periplasmic space"
/GO_process="GO:0009266 - response to temperature
stimulus"
/note="periplasmic serine protease Do; heat shock protein
HtrA"
/codon_start=1
/transl_table=11
/product="serine endoprotease (protease Do),
membrane-associated"
/protein_id="NP_414703.1"
/db_xref="GI:16128154"
/db_xref="ASAP:ABE-0000551"
/db_xref="UniProtKB/Swiss-Prot:P0C0V0"
/db_xref="EcoGene:EG10463"
/db_xref="GeneID:947139"
/translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
LDSKPSVLALNIQRGDSTIYLLMQ"
mat_peptide 180962..182305
/gene="degP"
/locus_tag="b0161"
/gene_synonym="ECK0160; htrA; JW0157; ptd"
/product="serine endoprotease (protease Do),
membrane-associated"
/experiment="N-terminus verified by Edman degradation:
PMID 2180903,9600841"
misc_feature 180989..182305
/gene="degP"
/locus_tag="b0161"
/gene_synonym="ECK0160; htrA; JW0157; ptd"
/note="serine endoprotease; Provisional; Region: PRK10942"
/db_xref="CDD:236802"
misc_feature 181223..181633
/gene="degP"
/locus_tag="b0161"
/gene_synonym="ECK0160; htrA; JW0157; ptd"
/note="Trypsin-like peptidase domain; Region: Trypsin_2;
pfam13365"
/db_xref="CDD:222077"
misc_feature 181754..182017
/gene="degP"
/locus_tag="b0161"
/gene_synonym="ECK0160; htrA; JW0157; ptd"
/note="PDZ domain of tryspin-like serine proteases, such
as DegP/HtrA, which are oligomeric proteins involved in
heat-shock response, chaperone function, and apoptosis.
May be responsible for substrate recognition and/or
binding, as most PDZ domains bind...; Region:
PDZ_serine_protease; cd00987"
/db_xref="CDD:238487"
misc_feature order(181754..181762,181766..181768,181919..181924,
181931..181936)
/gene="degP"
/locus_tag="b0161"
/gene_synonym="ECK0160; htrA; JW0157; ptd"
/note="protein binding site [polypeptide binding]; other
site"
/db_xref="CDD:238487"
misc_feature 182081..182296
/gene="degP"
/locus_tag="b0161"
/gene_synonym="ECK0160; htrA; JW0157; ptd"
/note="PDZ domain of tryspin-like serine proteases, such
as DegP/HtrA, which are oligomeric proteins involved in
heat-shock response, chaperone function, and apoptosis.
May be responsible for substrate recognition and/or
binding, as most PDZ domains bind...; Region:
PDZ_serine_protease; cd00987"
/db_xref="CDD:238487"
gene 182463..183620
/gene="cdaR"
/locus_tag="b0162"
/gene_synonym="ECK0161; JW5013; sdaR; yaeG"
/db_xref="EcoGene:EG12335"
/db_xref="GeneID:944860"
CDS 182463..183620
/gene="cdaR"
/locus_tag="b0162"
/gene_synonym="ECK0161; JW5013; sdaR; yaeG"
/codon_start=1
/transl_table=11
/product="DNA-binding transcriptional regulator for gar
and gud operons; carbohydrate diacid regulator"
/protein_id="NP_414704.4"
/db_xref="GI:90111093"
/db_xref="ASAP:ABE-0000554"
/db_xref="UniProtKB/Swiss-Prot:P37047"
/db_xref="EcoGene:EG12335"
/db_xref="GeneID:944860"
/translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKTMDNNGLLRRTLAAWFRHN
VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
misc_feature 182463..183617
/gene="cdaR"
/locus_tag="b0162"
/gene_synonym="ECK0161; JW5013; sdaR; yaeG"
/note="carbohydrate diacid transcriptional activator CdaR;
Provisional; Region: PRK11477"
/db_xref="CDD:183155"
misc_feature 182478..182882
/gene="cdaR"
/locus_tag="b0162"
/gene_synonym="ECK0161; JW5013; sdaR; yaeG"
/note="Putative sugar diacid recognition; Region:
Diacid_rec; pfam05651"
/db_xref="CDD:147680"
misc_feature 183438..183605
/gene="cdaR"
/locus_tag="b0162"
/gene_synonym="ECK0161; JW5013; sdaR; yaeG"
/note="PucR C-terminal helix-turn-helix domain; Region:
HTH_30; pfam13556"
/db_xref="CDD:205734"
gene complement(183709..184095)
/gene="yaeH"
/locus_tag="b0163"
/gene_synonym="ECK0162; JW0159"
/db_xref="EcoGene:EG12336"
/db_xref="GeneID:947044"
CDS complement(183709..184095)
/gene="yaeH"
/locus_tag="b0163"
/gene_synonym="ECK0162; JW0159"
/function="putative structure; Not classified"
/note="putative structural protein"
/codon_start=1
/transl_table=11
/product="conserved protein, UPF0325 family"
/protein_id="NP_414705.1"
/db_xref="GI:16128156"
/db_xref="ASAP:ABE-0000556"
/db_xref="UniProtKB/Swiss-Prot:P62768"
/db_xref="EcoGene:EG12336"
/db_xref="GeneID:947044"
/translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
DLRHLESVVTNKISEIEADLEKLTRK"
misc_feature complement(183721..184095)
/gene="yaeH"
/locus_tag="b0163"
/gene_synonym="ECK0162; JW0159"
/note="hypothetical protein; Provisional; Region:
PRK13677"
/db_xref="CDD:184234"
gene complement(184257..185069)
/gene="yaeI"
/locus_tag="b0164"
/gene_synonym="ECK0163; JW5014"
/db_xref="EcoGene:EG12337"
/db_xref="GeneID:945002"
CDS complement(184257..185069)
/gene="yaeI"
/locus_tag="b0164"
/gene_synonym="ECK0163; JW5014"
/codon_start=1
/transl_table=11
/product="phosphodiesterase with model substrate bis-pNPP"
/protein_id="NP_414706.2"
/db_xref="GI:90111094"
/db_xref="ASAP:ABE-0000559"
/db_xref="UniProtKB/Swiss-Prot:P37049"
/db_xref="EcoGene:EG12337"
/db_xref="GeneID:945002"
/translation="MISRRRFLQATAATIATSSGFGYMHYCEPGWFELIRHRLAFFKD
NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMSLNFSAFS
DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSAGITVLFNQATVIATPNRQFE
LVGTGDLWAGQCKPPPASEANLPRLVLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
misc_feature complement(184260..185069)
/gene="yaeI"
/locus_tag="b0164"
/gene_synonym="ECK0163; JW5014"
/note="phosphodiesterase YaeI; Provisional; Region:
PRK11340"
/db_xref="CDD:236899"
misc_feature complement(184263..184928)
/gene="yaeI"
/locus_tag="b0164"
/gene_synonym="ECK0163; JW5014"
/note="Bacillus subtilis YkuE and related proteins,
C-terminal metallophosphatase domain; Region: MPP_YkuE_C;
cd07385"
/db_xref="CDD:163628"
misc_feature complement(order(184437..184439,184443..184445,
184503..184505,184707..184712,184806..184808,
184896..184898,184902..184904))
/gene="yaeI"
/locus_tag="b0164"
/gene_synonym="ECK0163; JW5014"
/note="putative active site [active]"
/db_xref="CDD:163628"
misc_feature complement(order(184437..184439,184443..184445,
184503..184505,184710..184712,184806..184808,
184896..184898,184902..184904))
/gene="yaeI"
/locus_tag="b0164"
/gene_synonym="ECK0163; JW5014"
/note="putative metal binding site [ion binding]; other
site"
/db_xref="CDD:163628"
gene complement(185123..185947)
/gene="dapD"
/locus_tag="b0166"
/gene_synonym="ECK0164; JW0161; ssa"
/db_xref="EcoGene:EG10207"
/db_xref="GeneID:944862"
CDS complement(185123..185947)
/gene="dapD"
/locus_tag="b0166"
/gene_synonym="ECK0164; JW0161; ssa"
/EC_number="2.3.1.117"
/function="enzyme; Amino acid biosynthesis: Lysine"
/experiment="N-terminus verified by Edman degradation:
PMID 8740179,9298646"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0009089 - lysine biosynthetic process via
diaminopimelate"
/codon_start=1
/transl_table=11
/product="2,3,4,5-tetrahydropyridine-2-carboxylate
N-succinyltransferase"
/protein_id="NP_414708.1"
/db_xref="GI:16128159"
/db_xref="ASAP:ABE-0000565"
/db_xref="UniProtKB/Swiss-Prot:P0A9D8"
/db_xref="EcoGene:EG10207"
/db_xref="GeneID:944862"
/translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
misc_feature complement(185135..185947)
/gene="dapD"
/locus_tag="b0166"
/gene_synonym="ECK0164; JW0161; ssa"
/note="2,3,4,5-tetrahydropyridine-2,6-carboxylate
N-succinyltransferase; Provisional; Region: dapD;
PRK11830"
/db_xref="CDD:236996"
misc_feature complement(185231..185644)
/gene="dapD"
/locus_tag="b0166"
/gene_synonym="ECK0164; JW0161; ssa"
/note="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate (THDP)
N-succinyltransferase (also called THP
succinyltransferase): THDP N-succinyltransferase catalyzes
the conversion of tetrahydrodipicolinate and succinyl-CoA
to N-succinyltetrahydrodipicolinate and CoA; Region:
LbH_THP_succinylT; cd03350"
/db_xref="CDD:100041"
misc_feature complement(order(185231..185233,185237..185248,
185327..185329,185381..185383,185387..185389,
185465..185467,185513..185521,185567..185569,
185573..185575,185621..185626,185633..185638))
/gene="dapD"
/locus_tag="b0166"
/gene_synonym="ECK0164; JW0161; ssa"
/note="trimer interface [polypeptide binding]; other site"
/db_xref="CDD:100041"
misc_feature complement(order(185336..185341,185372..185374,
185387..185395,185432..185434,185441..185446,
185450..185452,185471..185473,185477..185479,
185504..185506,185525..185527,185561..185563,
185576..185578,185612..185614,185636..185638))
/gene="dapD"
/locus_tag="b0166"
/gene_synonym="ECK0164; JW0161; ssa"
/note="active site"
/db_xref="CDD:100041"
misc_feature complement(order(185444..185446,185504..185506,
185525..185527,185561..185563,185576..185578,
185612..185614,185636..185638))
/gene="dapD"
/locus_tag="b0166"
/gene_synonym="ECK0164; JW0161; ssa"
/note="substrate binding site [chemical binding]; other
site"
/db_xref="CDD:100041"
misc_feature complement(order(185336..185341,185372..185374,
185387..185395,185432..185434,185450..185452,
185468..185473))
/gene="dapD"
/locus_tag="b0166"
/gene_synonym="ECK0164; JW0161; ssa"
/note="CoA binding site [chemical binding]; other site"
/db_xref="CDD:100041"
gene complement(185978..188650)
/gene="glnD"
/locus_tag="b0167"
/gene_synonym="ECK0165; JW0162"
/db_xref="EcoGene:EG11411"
/db_xref="GeneID:944863"
CDS complement(185978..188650)
/gene="glnD"
/locus_tag="b0167"
/gene_synonym="ECK0165; JW0162"
/EC_number="2.7.7.59"
/function="enzyme; Amino acid biosynthesis: Glutamine"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006807 - nitrogen compound metabolic
process"
/GO_process="GO:0006542 - glutamine biosynthetic process"
/GO_process="GO:0006464 - protein modification process"
/note="protein PII; uridylyltransferase acts on regulator
of glnA"
/codon_start=1
/transl_table=11
/product="uridylyltransferase"
/protein_id="NP_414709.1"
/db_xref="GI:16128160"
/db_xref="ASAP:ABE-0000568"
/db_xref="UniProtKB/Swiss-Prot:P27249"
/db_xref="EcoGene:EG11411"
/db_xref="GeneID:944863"
/translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
LSDVDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVHNSAITGI
YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
FTTRDGMAMDTFIVLEPDGNPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
misc_feature complement(185981..188632)
/gene="glnD"
/locus_tag="b0167"
/gene_synonym="ECK0165; JW0162"
/note="PII uridylyl-transferase; Provisional; Region:
PRK05007"
/db_xref="CDD:235329"
misc_feature complement(188102..188500)
/gene="glnD"
/locus_tag="b0167"
/gene_synonym="ECK0165; JW0162"
/note="Nucleotidyltransferase (NT) domain of Escherichia
coli adenylyltransferase (GlnE), Escherichia coli uridylyl
transferase (GlnD), and similar proteins; Region:
NT_GlnE_GlnD_like; cd05401"
/db_xref="CDD:143391"
misc_feature complement(order(188252..188254,188330..188332,
188336..188338))
/gene="glnD"
/locus_tag="b0167"
/gene_synonym="ECK0165; JW0162"
/note="metal binding triad; metal-binding site"
/db_xref="CDD:143391"
misc_feature complement(187658..188077)
/gene="glnD"
/locus_tag="b0167"
/gene_synonym="ECK0165; JW0162"
/note="GlnD PII-uridylyltransferase; Region:
GlnD_UR_UTase; pfam08335"
/db_xref="CDD:219800"
misc_feature complement(186797..187255)
/gene="glnD"
/locus_tag="b0167"
/gene_synonym="ECK0165; JW0162"
/note="Metal dependent phosphohydrolases with conserved
'HD' motif; Region: HDc; cd00077"
/db_xref="CDD:238032"
misc_feature complement(order(186860..186862,187106..187111,
187238..187240))
/gene="glnD"
/locus_tag="b0167"
/gene_synonym="ECK0165; JW0162"
/note="Zn2+ binding site [ion binding]; other site"
/db_xref="CDD:238032"
misc_feature complement(187106..187108)
/gene="glnD"
/locus_tag="b0167"
/gene_synonym="ECK0165; JW0162"
/note="Mg2+ binding site [ion binding]; other site"
/db_xref="CDD:238032"
misc_feature complement(186317..186532)
/gene="glnD"
/locus_tag="b0167"
/gene_synonym="ECK0165; JW0162"
/note="ACT domain family, ACT_UUR-like_1, includes the
first of two C-terminal ACT domains of the bacterial
signal-transducing uridylyltransferase /uridylyl-removing
(UUR) enzyme, GlnD and related domains; Region:
ACT_UUR-like_1; cd04900"
/db_xref="CDD:153172"
misc_feature complement(185999..186202)
/gene="glnD"
/locus_tag="b0167"
/gene_synonym="ECK0165; JW0162"
/note="C-terminal ACT domains of the bacterial
signal-transducing uridylyltransferase /uridylyl-removing
(UUR) enzyme, GlnD and related domains; Region:
ACT_ACR-UUR-like_2; cd04899"
/db_xref="CDD:153171"
gene complement(188712..189506)
/gene="map"
/locus_tag="b0168"
/gene_synonym="ECK0166; JW0163; pepM"
/db_xref="EcoGene:EG10570"
/db_xref="GeneID:947882"
CDS complement(188712..189506)
/gene="map"
/locus_tag="b0168"
/gene_synonym="ECK0166; JW0163; pepM"
/EC_number="3.4.11.18"
/function="enzyme; Proteins - translation and
modification"
/GO_process="GO:0006457 - protein folding"
/codon_start=1
/transl_table=11
/product="methionine aminopeptidase"
/protein_id="NP_414710.1"
/db_xref="GI:16128161"
/db_xref="ASAP:ABE-0000571"
/db_xref="UniProtKB/Swiss-Prot:P0AE18"
/db_xref="EcoGene:EG10570"
/db_xref="GeneID:947882"
/translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
mat_peptide complement(188715..189503)
/gene="map"
/locus_tag="b0168"
/gene_synonym="ECK0166; JW0163; pepM"
/product="methionine aminopeptidase"
/experiment="N-terminus verified by Edman degradation:
PMID 3027045"
misc_feature complement(188757..189476)
/gene="map"
/locus_tag="b0168"
/gene_synonym="ECK0166; JW0163; pepM"
/note="Methionine Aminopeptidase 1. E.C. 3.4.11.18. Also
known as methionyl aminopeptidase and Peptidase M.
Catalyzes release of N-terminal amino acids,
preferentially methionine, from peptides and arylamides;
Region: MetAP1; cd01086"
/db_xref="CDD:238519"
misc_feature complement(order(188802..188804,188895..188897,
188994..188996,189183..189185,189216..189218,
189270..189272))
/gene="map"
/locus_tag="b0168"
/gene_synonym="ECK0166; JW0163; pepM"
/note="active site"
/db_xref="CDD:238519"
gene 189712..189847
/gene="tff"
/locus_tag="b4414"
/gene_synonym="ECK0167; JWR0225; t44"
/db_xref="EcoGene:EG31135"
/db_xref="GeneID:2847773"
ncRNA 189712..189847
/gene="tff"
/locus_tag="b4414"
/gene_synonym="ECK0167; JWR0225; t44"
/ncRNA_class="other"
/product="novel sRNA, function unknown"
/note="identified in a large scale screen"
/db_xref="ASAP:ABE-0047239"
/db_xref="EcoGene:EG31135"
/db_xref="GeneID:2847773"
gene 189874..190599
/gene="rpsB"
/locus_tag="b0169"
/gene_synonym="ECK0168; JW0164"
/db_xref="EcoGene:EG10901"
/db_xref="GeneID:947874"
CDS 189874..190599
/gene="rpsB"
/locus_tag="b0169"
/gene_synonym="ECK0168; JW0164"
/function="structural component; Ribosomal proteins -
synthesis, modification"
/GO_component="GO:0005737 - cytoplasm"
/GO_function="GO:0003735 - structural constituent of
ribosome"
/GO_process="GO:0006412 - translation"
/codon_start=1
/transl_table=11
/product="30S ribosomal subunit protein S2"
/protein_id="NP_414711.1"
/db_xref="GI:16128162"
/db_xref="ASAP:ABE-0000575"
/db_xref="UniProtKB/Swiss-Prot:P0A7V0"
/db_xref="EcoGene:EG10901"
/db_xref="GeneID:947874"
/translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
ATVREGRSQDLASQAEESFVEAE"
mat_peptide 189877..190596
/gene="rpsB"
/locus_tag="b0169"
/gene_synonym="ECK0168; JW0164"
/product="30S ribosomal subunit protein S2"
/experiment="N-terminus verified by Edman degradation:
PMID 1575737,7023985,9298646,10739205"
misc_feature 189898..190542
/gene="rpsB"
/locus_tag="b0169"
/gene_synonym="ECK0168; JW0164"
/note="Ribosomal protein S2 (RPS2), involved in formation
of the translation initiation complex, where it might
contact the messenger RNA and several components of the
ribosome. It has been shown that in Escherichia coli RPS2
is essential for the binding of...; Region: RPS2; cd01425"
/db_xref="CDD:100106"
misc_feature order(189946..189951,189976..189984,190156..190158,
190162..190170,190177..190182,190396..190398,
190405..190407)
/gene="rpsB"
/locus_tag="b0169"
/gene_synonym="ECK0168; JW0164"
/note="rRNA interaction site [nucleotide binding]; other
site"
/db_xref="CDD:100106"
misc_feature order(190402..190407,190411..190413,190453..190464)
/gene="rpsB"
/locus_tag="b0169"
/gene_synonym="ECK0168; JW0164"
/note="S8 interaction site; other site"
/db_xref="CDD:100106"
misc_feature 190507..190524
/gene="rpsB"
/locus_tag="b0169"
/gene_synonym="ECK0168; JW0164"
/note="putative laminin-1 binding site; other site"
/db_xref="CDD:100106"
gene 190857..191708
/gene="tsf"
/locus_tag="b0170"
/gene_synonym="ECK0169; JW0165"
/db_xref="EcoGene:EG11033"
/db_xref="GeneID:944866"
CDS 190857..191708
/gene="tsf"
/locus_tag="b0170"
/gene_synonym="ECK0169; JW0165"
/function="factor; Proteins - translation and
modification"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006412 - translation"
/codon_start=1
/transl_table=11
/product="protein chain elongation factor EF-Ts"
/protein_id="NP_414712.1"
/db_xref="GI:16128163"
/db_xref="ASAP:ABE-0000579"
/db_xref="UniProtKB/Swiss-Prot:P0A6P1"
/db_xref="EcoGene:EG11033"
/db_xref="GeneID:944866"
/translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
AAMSKQS"
misc_feature 190857..191699
/gene="tsf"
/locus_tag="b0170"
/gene_synonym="ECK0169; JW0165"
/note="elongation factor Ts; Provisional; Region: tsf;
PRK09377"
/db_xref="CDD:236491"
mat_peptide 190860..191705
/gene="tsf"
/locus_tag="b0170"
/gene_synonym="ECK0169; JW0165"
/product="protein chain elongation factor EF-Ts"
/experiment="N-terminus verified by Edman degradation:
PMID 8740179,9298646,9600841,9740056"
misc_feature 190866..190973
/gene="tsf"
/locus_tag="b0170"
/gene_synonym="ECK0169; JW0165"
/note="UBA/TS-N domain; Region: UBA; pfam00627"
/db_xref="CDD:201355"
misc_feature 191037..>191144
/gene="tsf"
/locus_tag="b0170"
/gene_synonym="ECK0169; JW0165"
/note="Elongation factor TS; Region: EF_TS; pfam00889"
/db_xref="CDD:189759"
misc_feature 191265..191645
/gene="tsf"
/locus_tag="b0170"
/gene_synonym="ECK0169; JW0165"
/note="Elongation factor TS; Region: EF_TS; pfam00889"
/db_xref="CDD:189759"
gene 191855..192580
/gene="pyrH"
/locus_tag="b0171"
/gene_synonym="ECK0170; JW0166; smbA; umk"
/db_xref="EcoGene:EG11539"
/db_xref="GeneID:944989"
CDS 191855..192580
/gene="pyrH"
/locus_tag="b0171"
/gene_synonym="ECK0170; JW0166; smbA; umk"
/EC_number="2.7.4.-"
/function="enzyme; Central intermediary metabolism:
Nucleotide interconversions"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0015949 - nucleobase, nucleoside and
nucleotide interconversion"
/codon_start=1
/transl_table=11
/product="uridylate kinase"
/protein_id="NP_414713.1"
/db_xref="GI:16128164"
/db_xref="ASAP:ABE-0000582"
/db_xref="UniProtKB/Swiss-Prot:P0A7E9"
/db_xref="EcoGene:EG11539"
/db_xref="GeneID:944989"
/translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
NMNKPGALRRVVMGEKEGTLITE"
mat_peptide 191858..192577
/gene="pyrH"
/locus_tag="b0171"
/gene_synonym="ECK0170; JW0166; smbA; umk"
/product="uridylate kinase"
/experiment="N-terminus verified by Edman degradation:
PMID 7711027"
misc_feature 191882..192574
/gene="pyrH"
/locus_tag="b0171"
/gene_synonym="ECK0170; JW0166; smbA; umk"
/note="UMP kinase (UMPK)-Ec, the microbial/chloroplast
uridine monophosphate kinase (uridylate kinase) enzyme
that catalyzes UMP phosphorylation and plays a key role in
pyrimidine nucleotide biosynthesis; regulation of this
process is via feed-back control and...; Region:
AAK_UMPK-PyrH-Ec; cd04254"
/db_xref="CDD:239787"
misc_feature order(191897..191899,191906..191911,192020..192028,
192287..192292,192347..192352,192359..192361,
192365..192367,192371..192376)
/gene="pyrH"
/locus_tag="b0171"
/gene_synonym="ECK0170; JW0166; smbA; umk"
/note="putative nucleotide binding site [chemical
binding]; other site"
/db_xref="CDD:239787"
misc_feature order(192020..192028,192038..192043,192083..192085,
192092..192097,192266..192268,192272..192277,
192281..192289)
/gene="pyrH"
/locus_tag="b0171"
/gene_synonym="ECK0170; JW0166; smbA; umk"
/note="uridine monophosphate binding site [chemical
binding]; other site"
/db_xref="CDD:239787"
misc_feature order(192059..192064,192086..192091,192110..192112,
192131..192133,192161..192163,192203..192205,
192215..192217,192224..192226,192242..192244,
192266..192268,192272..192280,192305..192307,
192314..192319,192485..192487)
/gene="pyrH"
/locus_tag="b0171"
/gene_synonym="ECK0170; JW0166; smbA; umk"
/note="homohexameric interface [polypeptide binding];
other site"
/db_xref="CDD:239787"
gene 192872..193429
/gene="frr"
/locus_tag="b0172"
/gene_synonym="ECK0171; JW0167; rrf"
/db_xref="EcoGene:EG10335"
/db_xref="GeneID:946122"
CDS 192872..193429
/gene="frr"
/locus_tag="b0172"
/gene_synonym="ECK0171; JW0167; rrf"
/function="factor; Proteins - translation and
modification"
/experiment="N-terminus verified by Edman degradation:
PMID 2684966,8740179,9298646"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006412 - translation"
/GO_process="GO:0009386 - translational attenuation"
/note="ribosome releasing factor"
/codon_start=1
/transl_table=11
/product="ribosome recycling factor"
/protein_id="NP_414714.1"
/db_xref="GI:16128165"
/db_xref="ASAP:ABE-0000588"
/db_xref="UniProtKB/Swiss-Prot:P0A805"
/db_xref="EcoGene:EG10335"
/db_xref="GeneID:946122"
/translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
QKLTDAAIKKIEAALADKEAELMQF"
misc_feature 192872..193426
/gene="frr"
/locus_tag="b0172"
/gene_synonym="ECK0171; JW0167; rrf"
/note="ribosome recycling factor; Reviewed; Region: frr;
PRK00083"
/db_xref="CDD:178850"
misc_feature 192884..193420
/gene="frr"
/locus_tag="b0172"
/gene_synonym="ECK0171; JW0167; rrf"
/note="Ribosome recycling factor (RRF). Ribosome recycling
factor dissociates the posttermination complex, composed
of the ribosome, deacylated tRNA, and mRNA, after
termination of translation. Thus ribosomes are 'recycled'
and ready for another...; Region: RRF; cd00520"
/db_xref="CDD:238288"
misc_feature order(192956..192967,193175..193186)
/gene="frr"
/locus_tag="b0172"
/gene_synonym="ECK0171; JW0167; rrf"
/note="hinge region; other site"
/db_xref="CDD:238288"
gene 193521..194717
/gene="dxr"
/locus_tag="b0173"
/gene_synonym="ECK0172; ispC; JW0168; yaeM"
/db_xref="EcoGene:EG12715"
/db_xref="GeneID:945019"
CDS 193521..194717
/gene="dxr"
/locus_tag="b0173"
/gene_synonym="ECK0172; ispC; JW0168; yaeM"
/EC_number="1.1.1.267"
/function="putative transport; Not classified"
/codon_start=1
/transl_table=11
/product="1-deoxy-D-xylulose 5-phosphate reductoisomerase"
/protein_id="NP_414715.1"
/db_xref="GI:16128166"
/db_xref="ASAP:ABE-0000592"
/db_xref="UniProtKB/Swiss-Prot:P45568"
/db_xref="EcoGene:EG12715"
/db_xref="GeneID:945019"
/translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
VMRLAS"
misc_feature 193521..194714
/gene="dxr"
/locus_tag="b0173"
/gene_synonym="ECK0172; ispC; JW0168; yaeM"
/note="1-deoxy-D-xylulose 5-phosphate reductoisomerase;
Provisional; Region: PRK05447"
/db_xref="CDD:235472"
misc_feature 193530..193916
/gene="dxr"
/locus_tag="b0173"
/gene_synonym="ECK0172; ispC; JW0168; yaeM"
/note="1-deoxy-D-xylulose 5-phosphate reductoisomerase;
Region: DXP_reductoisom; pfam02670"
/db_xref="CDD:217176"
misc_feature 193956..194237
/gene="dxr"
/locus_tag="b0173"
/gene_synonym="ECK0172; ispC; JW0168; yaeM"
/note="1-deoxy-D-xylulose 5-phosphate reductoisomerase
C-terminal; Region: DXP_redisom_C; pfam08436"
/db_xref="CDD:203943"
misc_feature 194334..194687
/gene="dxr"
/locus_tag="b0173"
/gene_synonym="ECK0172; ispC; JW0168; yaeM"
/note="DXP reductoisomerase C-terminal domain; Region:
DXPR_C; pfam13288"
/db_xref="CDD:222027"
gene 194903..195664
/gene="ispU"
/locus_tag="b0174"
/gene_synonym="ECK0173; JW0169; rth; uppS; yaeS"
/db_xref="EcoGene:EG13329"
/db_xref="GeneID:944874"
CDS 194903..195664
/gene="ispU"
/locus_tag="b0174"
/gene_synonym="ECK0173; JW0169; rth; uppS; yaeS"
/EC_number="2.5.1.31"
/experiment="N-terminus verified by Edman degradation:
PMID 9882662"
/GO_component="GO:0005737 - cytoplasm"
/GO_function="GO:0042280 - cell surface antigen activity,
host-interacting"
/note="undecaprenyl pyrophosphate synthetase
(di-trans,poly-cis-decaprenylcistransferase)"
/codon_start=1
/transl_table=11
/product="undecaprenyl pyrophosphate synthase"
/protein_id="NP_414716.1"
/db_xref="GI:16128167"
/db_xref="ASAP:ABE-0000595"
/db_xref="UniProtKB/Swiss-Prot:P60472"
/db_xref="EcoGene:EG13329"
/db_xref="GeneID:944874"
/translation="MMLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHK
AGAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHR
HNVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAE
KVQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTD
VLWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
misc_feature 194906..195640
/gene="ispU"
/locus_tag="b0174"
/gene_synonym="ECK0173; JW0169; rth; uppS; yaeS"
/note="Undecaprenyl pyrophosphate synthase [Lipid
metabolism]; Region: UppS; COG0020"
/db_xref="CDD:223099"
misc_feature 194954..195616
/gene="ispU"
/locus_tag="b0174"
/gene_synonym="ECK0173; JW0169; rth; uppS; yaeS"
/note="Cis (Z)-Isoprenyl Diphosphate Synthases (cis-IPPS);
homodimers which catalyze the successive 1'-4 condensation
of the isopentenyl diphosphate (IPP) molecule to
trans,trans-farnesyl diphosphate (FPP) or to cis,trans-FPP
to form long-chain polyprenyl...; Region: CIS_IPPS;
cd00475"
/db_xref="CDD:238265"
misc_feature 194978..194980
/gene="ispU"
/locus_tag="b0174"
/gene_synonym="ECK0173; JW0169; rth; uppS; yaeS"
/note="catalytic residue [active]"
/db_xref="CDD:238265"
misc_feature 194981..194992
/gene="ispU"
/locus_tag="b0174"
/gene_synonym="ECK0173; JW0169; rth; uppS; yaeS"
/note="putative FPP diphosphate binding site; other site"
/db_xref="CDD:238265"
misc_feature order(195101..195115,195155..195160,195164..195172,
195251..195253,195272..195274,195317..195319,
195323..195325)
/gene="ispU"
/locus_tag="b0174"
/gene_synonym="ECK0173; JW0169; rth; uppS; yaeS"
/note="putative FPP binding hydrophobic cleft; other site"
/db_xref="CDD:238265"
misc_feature order(195344..195346,195368..195370,195380..195382,
195389..195391,195416..195418,195497..195502,
195509..195511,195521..195523,195530..195532,
195542..195544)
/gene="ispU"
/locus_tag="b0174"
/gene_synonym="ECK0173; JW0169; rth; uppS; yaeS"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:238265"
misc_feature order(195482..195484,195500..195502)
/gene="ispU"
/locus_tag="b0174"
/gene_synonym="ECK0173; JW0169; rth; uppS; yaeS"
/note="putative IPP diphosphate binding site; other site"
/db_xref="CDD:238265"
gene 195677..196534
/gene="cdsA"
/locus_tag="b0175"
/gene_synonym="ECK0174; JW5810"
/db_xref="EcoGene:EG10139"
/db_xref="GeneID:944876"
CDS 195677..196534
/gene="cdsA"
/locus_tag="b0175"
/gene_synonym="ECK0174; JW5810"
/EC_number="2.7.7.41"
/function="enzyme; Fatty acid and phosphatidic acid
biosynthesis"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/GO_process="GO:0008654 - phospholipid biosynthetic
process"
/note="CDP-diglyceride synthetase"
/codon_start=1
/transl_table=11
/product="CDP-diglyceride synthase"
/protein_id="NP_414717.2"
/db_xref="GI:90111095"
/db_xref="ASAP:ABE-0000600"
/db_xref="UniProtKB/Swiss-Prot:P0ABG1"
/db_xref="EcoGene:EG10139"
/db_xref="GeneID:944876"
/translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
LLLLVFRTL"
misc_feature 195677..196531
/gene="cdsA"
/locus_tag="b0175"
/gene_synonym="ECK0174; JW5810"
/note="CDP-diglyceride synthase; Provisional; Region:
cdsA; PRK11624"
/db_xref="CDD:183240"
misc_feature 195677..196519
/gene="cdsA"
/locus_tag="b0175"
/gene_synonym="ECK0174; JW5810"
/note="CDP-diglyceride synthetase [Lipid metabolism];
Region: CdsA; COG0575"
/db_xref="CDD:223648"
gene 196546..197898
/gene="rseP"
/locus_tag="b0176"
/gene_synonym="ecfE; ECK0175; JW0171; yaeL"
/db_xref="EcoGene:EG12436"
/db_xref="GeneID:944871"
CDS 196546..197898
/gene="rseP"
/locus_tag="b0176"
/gene_synonym="ecfE; ECK0175; JW0171; yaeL"
/EC_number="3.4.24.-"
/codon_start=1
/transl_table=11
/product="inner membrane zinc RIP metalloprotease; RpoE
activator, by degrading RseA; cleaved signal peptide
endoprotease"
/protein_id="NP_414718.1"
/db_xref="GI:16128169"
/db_xref="ASAP:ABE-0000603"
/db_xref="UniProtKB/Swiss-Prot:P0AEH1"
/db_xref="EcoGene:EG12436"
/db_xref="GeneID:944871"
/translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
"
misc_feature 196549..197895
/gene="rseP"
/locus_tag="b0176"
/gene_synonym="ecfE; ECK0175; JW0171; yaeL"
/note="zinc metallopeptidase RseP; Provisional; Region:
PRK10779"
/db_xref="CDD:182723"
misc_feature 196567..>196899
/gene="rseP"
/locus_tag="b0176"
/gene_synonym="ecfE; ECK0175; JW0171; yaeL"
/note="RseP-like Site-2 proteases (S2P), zinc
metalloproteases (MEROPS family M50A), cleave
transmembrane domains of substrate proteins, regulating
intramembrane proteolysis (RIP) of diverse signal
transduction mechanisms. In Escherichia coli, the S2P
homolog...; Region: S2P-M50_PDZ_RseP-like; cd06163"
/db_xref="CDD:100084"
misc_feature order(196609..196614,196621..196623)
/gene="rseP"
/locus_tag="b0176"
/gene_synonym="ecfE; ECK0175; JW0171; yaeL"
/note="active site"
/db_xref="CDD:100084"
misc_feature 197176..197412
/gene="rseP"
/locus_tag="b0176"
/gene_synonym="ecfE; ECK0175; JW0171; yaeL"
/note="PDZ domain of bacterial and plant zinc
metalloprotases, presumably membrane-associated or
integral membrane proteases, which may be involved in
signalling and regulatory mechanisms. May be responsible
for substrate recognition and/or binding, as most PDZ...;
Region: PDZ_metalloprotease; cd00989"
/db_xref="CDD:238489"
misc_feature order(197179..197190,197194..197196,197323..197328,
197335..197340)
/gene="rseP"
/locus_tag="b0176"
/gene_synonym="ecfE; ECK0175; JW0171; yaeL"
/note="protein binding site [polypeptide binding]; other
site"
/db_xref="CDD:238489"
misc_feature <197683..197892
/gene="rseP"
/locus_tag="b0176"
/gene_synonym="ecfE; ECK0175; JW0171; yaeL"
/note="RseP-like Site-2 proteases (S2P), zinc
metalloproteases (MEROPS family M50A), cleave
transmembrane domains of substrate proteins, regulating
intramembrane proteolysis (RIP) of diverse signal
transduction mechanisms. In Escherichia coli, the S2P
homolog...; Region: S2P-M50_PDZ_RseP-like; cd06163"
/db_xref="CDD:100084"
misc_feature 197725..197736
/gene="rseP"
/locus_tag="b0176"
/gene_synonym="ecfE; ECK0175; JW0171; yaeL"
/note="putative substrate binding region [chemical
binding]; other site"
/db_xref="CDD:100084"
gene 197928..200360
/gene="bamA"
/locus_tag="b0177"
/gene_synonym="ecfK; ECK0176; JW0172; omp85; yaeT; yzzN;
yzzY"
/db_xref="EcoGene:EG12676"
/db_xref="GeneID:944870"
CDS 197928..200360
/gene="bamA"
/locus_tag="b0177"
/gene_synonym="ecfK; ECK0176; JW0172; omp85; yaeT; yzzN;
yzzY"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0009279 - cell outer membrane"
/note="putative outer membrane antigen"
/codon_start=1
/transl_table=11
/product="outer membrane protein assembly factor, forms
pores; required for OM biogenesis; in BamABCDE OM protein
complex"
/protein_id="NP_414719.1"
/db_xref="GI:16128170"
/db_xref="ASAP:ABE-0000605"
/db_xref="UniProtKB/Swiss-Prot:P0A940"
/db_xref="EcoGene:EG12676"
/db_xref="GeneID:944870"
/translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
AEQFQFNIGKTW"
misc_feature 197928..200357
/gene="bamA"
/locus_tag="b0177"
/gene_synonym="ecfK; ECK0176; JW0172; omp85; yaeT; yzzN;
yzzY"
/note="outer membrane protein assembly factor YaeT;
Provisional; Region: PRK11067"
/db_xref="CDD:236834"
mat_peptide 197988..200357
/gene="bamA"
/locus_tag="b0177"
/gene_synonym="ecfK; ECK0176; JW0172; omp85; yaeT; yzzN;
yzzY"
/product="outer membrane protein assembly factor, forms
pores; required for OM biogenesis; in BamABCDE OM protein
complex"
/experiment="N-terminus verified by Edman degradation:
PMID 9298646"
misc_feature 197997..198200
/gene="bamA"
/locus_tag="b0177"
/gene_synonym="ecfK; ECK0176; JW0172; omp85; yaeT; yzzN;
yzzY"
/note="Surface antigen variable number repeat; Region:
Surf_Ag_VNR; pfam07244"
/db_xref="CDD:219346"
misc_feature 198201..198443
/gene="bamA"
/locus_tag="b0177"
/gene_synonym="ecfK; ECK0176; JW0172; omp85; yaeT; yzzN;
yzzY"
/note="Surface antigen variable number repeat; Region:
Surf_Ag_VNR; pfam07244"
/db_xref="CDD:219346"
misc_feature 198450..198716
/gene="bamA"
/locus_tag="b0177"
/gene_synonym="ecfK; ECK0176; JW0172; omp85; yaeT; yzzN;
yzzY"
/note="Surface antigen variable number repeat; Region:
Surf_Ag_VNR; pfam07244"
/db_xref="CDD:219346"
misc_feature 198723..198959
/gene="bamA"
/locus_tag="b0177"
/gene_synonym="ecfK; ECK0176; JW0172; omp85; yaeT; yzzN;
yzzY"
/note="Surface antigen variable number repeat; Region:
Surf_Ag_VNR; pfam07244"
/db_xref="CDD:219346"
misc_feature 198966..199190
/gene="bamA"
/locus_tag="b0177"
/gene_synonym="ecfK; ECK0176; JW0172; omp85; yaeT; yzzN;
yzzY"
/note="Surface antigen variable number repeat; Region:
Surf_Ag_VNR; pfam07244"
/db_xref="CDD:219346"
misc_feature 199269..200357
/gene="bamA"
/locus_tag="b0177"
/gene_synonym="ecfK; ECK0176; JW0172; omp85; yaeT; yzzN;
yzzY"
/note="Surface antigen; Region: Bac_surface_Ag; pfam01103"
/db_xref="CDD:216300"
gene 200482..200967
/gene="skp"
/locus_tag="b0178"
/gene_synonym="ECK0177; hlpA; JW0173; ompH"
/db_xref="EcoGene:EG10455"
/db_xref="GeneID:944861"
CDS 200482..200967
/gene="skp"
/locus_tag="b0178"
/gene_synonym="ECK0177; hlpA; JW0173; ompH"
/function="factor; Basic proteins - synthesis,
modification"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0042597 - periplasmic space"
/GO_process="GO:0006457 - protein folding"
/note="periplasmic molecular chaperone for outer membrane
proteins"
/codon_start=1
/transl_table=11
/product="periplasmic chaperone"
/protein_id="NP_414720.1"
/db_xref="GI:16128171"
/db_xref="ASAP:ABE-0000608"
/db_xref="UniProtKB/Swiss-Prot:P0AEU7"
/db_xref="EcoGene:EG10455"
/db_xref="GeneID:944861"
/translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
K"
mat_peptide 200542..200964
/gene="skp"
/locus_tag="b0178"
/gene_synonym="ECK0177; hlpA; JW0173; ompH"
/product="periplasmic chaperone"
/experiment="N-terminus verified by Edman degradation:
PMID 2167239,2843433,9298646"
misc_feature 200545..200964
/gene="skp"
/locus_tag="b0178"
/gene_synonym="ECK0177; hlpA; JW0173; ompH"
/note="periplasmic chaperone; Provisional; Region:
PRK10780"
/db_xref="CDD:182724"
misc_feature 200551..200964
/gene="skp"
/locus_tag="b0178"
/gene_synonym="ECK0177; hlpA; JW0173; ompH"
/note="Outer membrane protein (OmpH-like); Region: OmpH;
smart00935"
/db_xref="CDD:214922"
gene 200971..201996
/gene="lpxD"
/locus_tag="b0179"
/gene_synonym="ECK0178; firA; JW0174; omsA; ssc"
/db_xref="EcoGene:EG10316"
/db_xref="GeneID:944882"
CDS 200971..201996
/gene="lpxD"
/locus_tag="b0179"
/gene_synonym="ECK0178; firA; JW0174; omsA; ssc"
/EC_number="2.3.1.-"
/function="enzyme; Surface polysaccharides & antigens"
/GO_function="GO:0042280 - cell surface antigen activity,
host-interacting"
/GO_process="GO:0009245 - lipid A biosynthetic process"
/codon_start=1
/transl_table=11
/product="UDP-3-O-(3-hydroxymyristoyl)-glucosamine
N-acyltransferase"
/protein_id="NP_414721.1"
/db_xref="GI:16128172"
/db_xref="ASAP:ABE-0000610"
/db_xref="UniProtKB/Swiss-Prot:P21645"
/db_xref="EcoGene:EG10316"
/db_xref="GeneID:944882"
/translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
RKVNQQD"
mat_peptide 200974..201993
/gene="lpxD"
/locus_tag="b0179"
/gene_synonym="ECK0178; firA; JW0174; omsA; ssc"
/product="UDP-3-O-(3-hydroxymyristoyl)-glucosamine
N-acyltransferase"
/experiment="N-terminus verified by Edman degradation:
PMID 2115869"
misc_feature 201016..201990
/gene="lpxD"
/locus_tag="b0179"
/gene_synonym="ECK0178; firA; JW0174; omsA; ssc"
/note="UDP-3-O-[3-hydroxymyristoyl] glucosamine
N-acyltransferase; Provisional; Region: lpxD; PRK00892"
/db_xref="CDD:234858"
misc_feature 201031..201240
/gene="lpxD"
/locus_tag="b0179"
/gene_synonym="ECK0178; firA; JW0174; omsA; ssc"
/note="UDP-3-O-[3-hydroxymyristoyl] glucosamine
N-acyltransferase, LpxD; Region: LpxD; pfam04613"
/db_xref="CDD:218175"
misc_feature 201295..201912
/gene="lpxD"
/locus_tag="b0179"
/gene_synonym="ECK0178; firA; JW0174; omsA; ssc"
/note="UDP-3-O-acyl-glucosamine N-acyltransferase (LpxD):
The enzyme catalyzes the transfer of 3-hydroxymyristic
acid or 3-hydroxy-arachidic acid, depending on the
organism, from the acyl carrier protein (ACP) to
UDP-3-O-acyl-glucosamine to produce UDP-2; Region:
LbH_LpxD; cd03352"
/db_xref="CDD:100043"
misc_feature order(201331..201333,201343..201348,201400..201402,
201439..201441,201445..201447,201451..201453,
201487..201489,201493..201495,201511..201513,
201517..201519,201550..201558,201592..201594,
201604..201606,201610..201612,201616..201618,
201625..201633,201658..201660,201664..201672,
201676..201678,201688..201690,201706..201708,
201712..201714,201724..201732,201760..201762,
201778..201786,201796..201798,201832..201840,
201892..201894,201904..201906)
/gene="lpxD"
/locus_tag="b0179"
/gene_synonym="ECK0178; firA; JW0174; omsA; ssc"
/note="trimer interface [polypeptide binding]; other site"
/db_xref="CDD:100043"
misc_feature order(201517..201525,201664..201666,201676..201678,
201685..201690,201718..201723,201736..201744,
201793..201798,201829..201831)
/gene="lpxD"
/locus_tag="b0179"
/gene_synonym="ECK0178; firA; JW0174; omsA; ssc"
/note="active site"
/db_xref="CDD:100043"
misc_feature order(201517..201525,201685..201690,201739..201744,
201793..201798)
/gene="lpxD"
/locus_tag="b0179"
/gene_synonym="ECK0178; firA; JW0174; omsA; ssc"
/note="UDP-GlcNAc binding site [chemical binding]; other
site"
/db_xref="CDD:100043"
misc_feature order(201664..201666,201676..201678,201718..201723,
201736..201738,201829..201831)
/gene="lpxD"
/locus_tag="b0179"
/gene_synonym="ECK0178; firA; JW0174; omsA; ssc"
/note="lipid binding site [chemical binding];
lipid-binding site"
/db_xref="CDD:100043"
gene 202101..202556
/gene="fabZ"
/locus_tag="b0180"
/gene_synonym="ECK0179; JW0175; sabA; sefA; sfhC; yaeA"
/db_xref="EcoGene:EG11284"
/db_xref="GeneID:944888"
CDS 202101..202556
/gene="fabZ"
/locus_tag="b0180"
/gene_synonym="ECK0179; JW0175; sabA; sefA; sfhC; yaeA"
/EC_number="4.2.1.-"
/function="enzyme; Fatty acid and phosphatidic acid
biosynthesis"
/GO_component="GO:0005737 - cytoplasm"
/codon_start=1
/transl_table=11
/product="(3R)-hydroxymyristol acyl carrier protein
dehydratase"
/protein_id="NP_414722.1"
/db_xref="GI:16128173"
/db_xref="ASAP:ABE-0000613"
/db_xref="UniProtKB/Swiss-Prot:P0A6Q6"
/db_xref="EcoGene:EG11284"
/db_xref="GeneID:944888"
/translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
mat_peptide 202104..202553
/gene="fabZ"
/locus_tag="b0180"
/gene_synonym="ECK0179; JW0175; sabA; sefA; sfhC; yaeA"
/product="(3R)-hydroxymyristol acyl carrier protein
dehydratase"
/experiment="N-terminus verified by Edman degradation:
PMID 8354462"
misc_feature 202149..202538
/gene="fabZ"
/locus_tag="b0180"
/gene_synonym="ECK0179; JW0175; sabA; sefA; sfhC; yaeA"
/note="FabZ is a 17kD beta-hydroxyacyl-acyl carrier
protein (ACP) dehydratase that primarily catalyzes the
dehydration of beta-hydroxyacyl-ACP to trans-2-acyl-ACP,
the third step in the elongation phase of the bacterial/
plastid, type II, fatty-acid...; Region: FabZ; cd01288"
/db_xref="CDD:238615"
gene 202560..203348
/gene="lpxA"
/locus_tag="b0181"
/gene_synonym="ECK0180; JW0176"
/db_xref="EcoGene:EG10545"
/db_xref="GeneID:944849"
CDS 202560..203348
/gene="lpxA"
/locus_tag="b0181"
/gene_synonym="ECK0180; JW0176"
/EC_number="2.3.1.129"
/function="enzyme; Surface polysaccharides & antigens"
/experiment="N-terminus verified by Edman degradation:
PMID 8366124"
/GO_component="GO:0005737 - cytoplasm"
/GO_function="GO:0042280 - cell surface antigen activity,
host-interacting"
/GO_process="GO:0009245 - lipid A biosynthetic process"
/note="UDP-N-acetylglucosamine acetyltransferase; lipid A
biosynthesis"
/codon_start=1
/transl_table=11
/product="UDP-N-acetylglucosamine acetyltransferase"
/protein_id="NP_414723.1"
/db_xref="GI:16128174"
/db_xref="ASAP:ABE-0000618"
/db_xref="UniProtKB/Swiss-Prot:P0A722"
/db_xref="EcoGene:EG10545"
/db_xref="GeneID:944849"
/translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
misc_feature 202569..203345
/gene="lpxA"
/locus_tag="b0181"
/gene_synonym="ECK0180; JW0176"
/note="UDP-N-acetylglucosamine acyltransferase;
Provisional; Region: PRK05289"
/db_xref="CDD:235390"
misc_feature 202581..203339
/gene="lpxA"
/locus_tag="b0181"
/gene_synonym="ECK0180; JW0176"
/note="UDP-N-acetylglucosamine O-acyltransferase
(UDP-GlcNAc acyltransferase): Proteins in this family
catalyze the transfer of (R)-3-hydroxymyristic acid from
its acyl carrier protein thioester to UDP-GlcNAc. It is
the first enzyme in the lipid A biosynthetic...; Region:
LbH_UDP-GlcNAc_AT; cd03351"
/db_xref="CDD:100042"
misc_feature order(202776..202778,202785..202787,202854..202856,
202923..202925,202932..202934,202989..202991,
203037..203042,203076..203078,203151..203153,
203169..203174)
/gene="lpxA"
/locus_tag="b0181"
/gene_synonym="ECK0180; JW0176"
/note="active site"
/db_xref="CDD:100042"
gene 203348..204496
/gene="lpxB"
/locus_tag="b0182"
/gene_synonym="ECK0181; JW0177; pgsB"
/db_xref="EcoGene:EG10546"
/db_xref="GeneID:944838"
CDS 203348..204496
/gene="lpxB"
/locus_tag="b0182"
/gene_synonym="ECK0181; JW0177; pgsB"
/EC_number="2.4.1.182"
/function="enzyme; Macromolecule metabolism:
Lipopolysaccharide"
/experiment="N-terminus verified by Edman degradation:
PMID 3049593"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0005737 - cytoplasm"
/GO_function="GO:0042280 - cell surface antigen activity,
host-interacting"
/GO_process="GO:0009245 - lipid A biosynthetic process"
/note="tetraacyldisaccharide-1-P; lipid A biosynthesis,
penultimate step"
/codon_start=1
/transl_table=11
/product="tetraacyldisaccharide-1-P synthase"
/protein_id="NP_414724.1"
/db_xref="GI:16128175"
/db_xref="ASAP:ABE-0000620"
/db_xref="UniProtKB/Swiss-Prot:P10441"
/db_xref="EcoGene:EG10546"
/db_xref="GeneID:944838"
/translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
misc_feature 203363..204451
/gene="lpxB"
/locus_tag="b0182"
/gene_synonym="ECK0181; JW0177; pgsB"
/note="lipid-A-disaccharide synthase; Reviewed; Region:
lpxB; PRK00025"
/db_xref="CDD:234580"
misc_feature 203369..204340
/gene="lpxB"
/locus_tag="b0182"
/gene_synonym="ECK0181; JW0177; pgsB"
/note="ipid-A-disaccharide synthase; Provisional; Region:
PRK14089"
/db_xref="CDD:237606"
gene 204493..205089
/gene="rnhB"
/locus_tag="b0183"
/gene_synonym="ECK0182; JW0178"
/db_xref="EcoGene:EG10861"
/db_xref="GeneID:944852"
CDS 204493..205089
/gene="rnhB"
/locus_tag="b0183"
/gene_synonym="ECK0182; JW0178"
/EC_number="3.1.26.4"
/function="enzyme; Degradation of RNA"
/experiment="N-terminus verified by Edman degradation:
PMID 2172991"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006401 - RNA catabolic process"
/note="RNAse HII, degrades RNA of DNA-RNA hybrids"
/codon_start=1
/transl_table=11
/product="ribonuclease HII, degrades RNA of DNA-RNA
hybrids"
/protein_id="NP_414725.1"
/db_xref="GI:16128176"
/db_xref="ASAP:ABE-0000622"
/db_xref="UniProtKB/Swiss-Prot:P10442"
/db_xref="EcoGene:EG10861"
/db_xref="GeneID:944852"
/translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
misc_feature 204529..205065
/gene="rnhB"
/locus_tag="b0183"
/gene_synonym="ECK0182; JW0178"
/note="bacterial Ribonuclease HII-like; Region:
RNase_HII_bacteria_HII_like; cd07182"
/db_xref="CDD:187695"
misc_feature order(204538..204549,204739..204747,204754..204756,
204814..204816,204853..204855,204910..204912)
/gene="rnhB"
/locus_tag="b0183"
/gene_synonym="ECK0182; JW0178"
/note="RNA/DNA hybrid binding site [nucleotide binding];
other site"
/db_xref="CDD:187695"
misc_feature order(204538..204543,204814..204816,204868..204870)
/gene="rnhB"
/locus_tag="b0183"
/gene_synonym="ECK0182; JW0178"
/note="active site"
/db_xref="CDD:187695"
gene 205126..208608
/gene="dnaE"
/locus_tag="b0184"
/gene_synonym="ECK0183; JW0179; polC; sdgC"
/db_xref="EcoGene:EG10238"
/db_xref="GeneID:944877"
CDS 205126..208608
/gene="dnaE"
/locus_tag="b0184"
/gene_synonym="ECK0183; JW0179; polC; sdgC"
/EC_number="2.7.7.7"
/function="enzyme; DNA - replication, repair,
restriction/modification"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006261 - DNA-dependent DNA replication"
/note="DNA polymerase III, alpha subunit"
/codon_start=1
/transl_table=11
/product="DNA polymerase III alpha subunit"
/protein_id="NP_414726.1"
/db_xref="GI:16128177"
/db_xref="ASAP:ABE-0000624"
/db_xref="UniProtKB/Swiss-Prot:P10443"
/db_xref="EcoGene:EG10238"
/db_xref="GeneID:944877"
/translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
RGLIGSEQVELEFD"
misc_feature 205138..208578
/gene="dnaE"
/locus_tag="b0184"
/gene_synonym="ECK0183; JW0179; polC; sdgC"
/note="DNA polymerase III subunit alpha; Validated;
Region: dnaE; PRK05673"
/db_xref="CDD:235554"
misc_feature 205138..205968
/gene="dnaE"
/locus_tag="b0184"
/gene_synonym="ECK0183; JW0179; polC; sdgC"
/note="Polymerase and Histidinol Phosphatase domain of
alpha-subunit of bacterial polymerase III DnaE1; Region:
PHP_PolIIIA_DnaE1; cd07433"
/db_xref="CDD:213988"
misc_feature order(205153..205155,205159..205161,205180..205182,
205255..205257,205330..205332,205726..205728,
205732..205734)
/gene="dnaE"
/locus_tag="b0184"
/gene_synonym="ECK0183; JW0179; polC; sdgC"
/note="putative active site [active]"
/db_xref="CDD:213988"
misc_feature order(205297..205299,205309..205317,205927..205929)
/gene="dnaE"
/locus_tag="b0184"
/gene_synonym="ECK0183; JW0179; polC; sdgC"
/note="putative PHP Thumb interface [polypeptide binding];
other site"
/db_xref="CDD:213988"
misc_feature 208108..208365
/gene="dnaE"
/locus_tag="b0184"
/gene_synonym="ECK0183; JW0179; polC; sdgC"
/note="DnaE_OBF: A subfamily of OB folds corresponding to
the C-terminal OB-fold nucleic acid binding domain of
Thermus aquaticus and Escherichia coli type C replicative
DNA polymerase III alpha subunit (DnaE). The DNA
polymerase holoenzyme of E. coli contains...; Region:
DnaE_OBF; cd04485"
/db_xref="CDD:239931"
misc_feature order(208108..208110,208264..208266,208270..208272,
208276..208278,208336..208338)
/gene="dnaE"
/locus_tag="b0184"
/gene_synonym="ECK0183; JW0179; polC; sdgC"
/note="generic binding surface II; other site"
/db_xref="CDD:239931"
misc_feature order(208123..208131,208171..208182,208186..208188,
208204..208212,208216..208218,208261..208263,
208282..208290,208315..208323)
/gene="dnaE"
/locus_tag="b0184"
/gene_synonym="ECK0183; JW0179; polC; sdgC"
/note="generic binding surface I; other site"
/db_xref="CDD:239931"
gene 208621..209580
/gene="accA"
/locus_tag="b0185"
/gene_synonym="ECK0184; JW0180"
/db_xref="EcoGene:EG11647"
/db_xref="GeneID:944895"
CDS 208621..209580
/gene="accA"
/locus_tag="b0185"
/gene_synonym="ECK0184; JW0180"
/EC_number="6.4.1.2"
/function="enzyme; Fatty acid and phosphatidic acid
biosynthesis"
/GO_component="GO:0005737 - cytoplasm"
/note="acetylCoA carboxylase, carboxytransferase
component, alpha subunit"
/codon_start=1
/transl_table=11
/product="acetyl-CoA carboxylase, carboxytransferase,
alpha subunit"
/protein_id="NP_414727.1"
/db_xref="GI:16128178"
/db_xref="ASAP:ABE-0000630"
/db_xref="UniProtKB/Swiss-Prot:P0ABD5"
/db_xref="EcoGene:EG11647"
/db_xref="GeneID:944895"
/translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
misc_feature 208621..209574
/gene="accA"
/locus_tag="b0185"
/gene_synonym="ECK0184; JW0180"
/note="acetyl-CoA carboxylase carboxyltransferase subunit
alpha; Validated; Region: PRK05724"
/db_xref="CDD:235580"
misc_feature 208621..209574
/gene="accA"
/locus_tag="b0185"
/gene_synonym="ECK0184; JW0180"
/note="acetyl-CoA carboxylase carboxyltransferase alpha
subunit; Provisional; Region: accA; CHL00198"
/db_xref="CDD:214393"
mat_peptide 208624..209577
/gene="accA"
/locus_tag="b0185"
/gene_synonym="ECK0184; JW0180"
/product="acetyl-CoA carboxylase, carboxytransferase,
alpha subunit"
/experiment="N-terminus verified by Edman degradation:
PMID 1355089,9298646"
gene 209679..211820
/gene="ldcC"
/locus_tag="b0186"
/gene_synonym="ECK0185; JW0181; ldc; LDC2; ldcH"
/db_xref="EcoGene:EG13219"
/db_xref="GeneID:944887"
CDS 209679..211820
/gene="ldcC"
/locus_tag="b0186"
/gene_synonym="ECK0185; JW0181; ldc; LDC2; ldcH"
/EC_number="4.1.1.18"
/function="enzyme; Energy metabolism, carbon: Pyruvate
dehydrogenase"
/experiment="N-terminus verified by Edman degradation:
PMID 9226257,9339543"
/GO_process="GO:0006096 - glycolysis"
/GO_process="GO:0016052 - carbohydrate catabolic process"
/codon_start=1
/transl_table=11
/product="lysine decarboxylase 2, constitutive"
/protein_id="NP_414728.1"
/db_xref="GI:16128179"
/db_xref="ASAP:ABE-0000633"
/db_xref="UniProtKB/Swiss-Prot:P52095"
/db_xref="EcoGene:EG13219"
/db_xref="GeneID:944887"
/translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
misc_feature 209679..211817
/gene="ldcC"
/locus_tag="b0186"
/gene_synonym="ECK0185; JW0181; ldc; LDC2; ldcH"
/note="lysine decarboxylase LdcC; Provisional; Region:
PRK15399"
/db_xref="CDD:185297"
misc_feature 209718..210050
/gene="ldcC"
/locus_tag="b0186"
/gene_synonym="ECK0185; JW0181; ldc; LDC2; ldcH"
/note="Orn/Lys/Arg decarboxylase, N-terminal domain;
Region: OKR_DC_1_N; pfam03709"
/db_xref="CDD:217683"
misc_feature 210069..211001
/gene="ldcC"
/locus_tag="b0186"
/gene_synonym="ECK0185; JW0181; ldc; LDC2; ldcH"
/note="Ornithine decarboxylase family. This family belongs
to pyridoxal phosphate (PLP)-dependent aspartate
aminotransferase superfamily (fold I). The major groups in
this CD corresponds to ornithine decarboxylase (ODC),
arginine decarboxylase (ADC) and lysine...; Region:
Orn_deC_like; cd00615"
/db_xref="CDD:99739"
misc_feature order(210213..210215,210228..210230,210252..210254,
210327..210335,210339..210344,210351..210353,
210363..210371,210375..210380,210411..210416,
210774..210779,210786..210800,210849..210854,
210858..210866,210870..210872,210891..210893,
210900..210902,210909..210911,210948..210953)
/gene="ldcC"
/locus_tag="b0186"
/gene_synonym="ECK0185; JW0181; ldc; LDC2; ldcH"
/note="homodimer interface [polypeptide binding]; other
site"
/db_xref="CDD:99739"
misc_feature order(210333..210341,210411..210413,210417..210419,
210588..210590,210666..210668,210672..210677,
210768..210770,210774..210779)
/gene="ldcC"
/locus_tag="b0186"
/gene_synonym="ECK0185; JW0181; ldc; LDC2; ldcH"
/note="pyridoxal 5'-phosphate binding site [chemical
binding]; other site"
/db_xref="CDD:99739"
misc_feature 210777..210779
/gene="ldcC"
/locus_tag="b0186"
/gene_synonym="ECK0185; JW0181; ldc; LDC2; ldcH"
/note="catalytic residue [active]"
/db_xref="CDD:99739"
misc_feature 211386..211790
/gene="ldcC"
/locus_tag="b0186"
/gene_synonym="ECK0185; JW0181; ldc; LDC2; ldcH"
/note="Orn/Lys/Arg decarboxylase, C-terminal domain;
Region: OKR_DC_1_C; pfam03711"
/db_xref="CDD:112521"
gene 211877..212266
/gene="yaeR"
/locus_tag="b0187"
/gene_synonym="ECK0186; JW0182"
/db_xref="EcoGene:EG13224"
/db_xref="GeneID:944875"
CDS 211877..212266
/gene="yaeR"
/locus_tag="b0187"
/gene_synonym="ECK0186; JW0182"
/codon_start=1
/transl_table=11
/product="putative lyase"
/protein_id="NP_414729.4"
/db_xref="GI:90111096"
/db_xref="ASAP:ABE-0000635"
/db_xref="UniProtKB/Swiss-Prot:P52096"
/db_xref="EcoGene:EG13224"
/db_xref="GeneID:944875"
/translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCET
IRVDPYTQKRFTFFNDPDGLPLELYEQ"
misc_feature 211886..212260
/gene="yaeR"
/locus_tag="b0187"
/gene_synonym="ECK0186; JW0182"
/note="This conserved domain belongs to a superfamily
including the bleomycin resistance protein, glyoxalase I,
and type I ring-cleaving dioxygenases; Region:
Glo_EDI_BRP_like_1; cd08352"
/db_xref="CDD:211358"
misc_feature 211892..212254
/gene="yaeR"
/locus_tag="b0187"
/gene_synonym="ECK0186; JW0182"
/note="Glyoxalase/Bleomycin resistance protein/Dioxygenase
superfamily; Region: Glyoxalase; pfam00903"
/db_xref="CDD:216182"
misc_feature order(211901..211903,212108..212110,212249..212251)
/gene="yaeR"
/locus_tag="b0187"
/gene_synonym="ECK0186; JW0182"
/note="putative metal binding site [ion binding]; other
site"
/db_xref="CDD:211358"
gene 212331..213629
/gene="tilS"
/locus_tag="b0188"
/gene_synonym="ECK0187; JW0183; mesJ; yaeN"
/db_xref="EcoGene:EG13220"
/db_xref="GeneID:944889"
CDS 212331..213629
/gene="tilS"
/locus_tag="b0188"
/gene_synonym="ECK0187; JW0183; mesJ; yaeN"
/EC_number="6.1.1.5"
/function="enzyme; tRNA modification; responsible for
lysidine formation in tRNA-Ile anticodon (C34 to L34)"
/codon_start=1
/transl_table=11
/product="tRNA(Ile)-lysidine synthetase"
/protein_id="NP_414730.1"
/db_xref="GI:16128181"
/db_xref="ASAP:ABE-0000638"
/db_xref="UniProtKB/Swiss-Prot:P52097"
/db_xref="EcoGene:EG13220"
/db_xref="GeneID:944889"
/translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVAL
RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
GELVQWARQYDLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
QESLLDELLADDLAHCQSPQGTLQIVPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSENIVPWQTWLQPLELPA
GLGSVQLNAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
misc_feature 212331..213626
/gene="tilS"
/locus_tag="b0188"
/gene_synonym="ECK0187; JW0183; mesJ; yaeN"
/note="tRNA(Ile)-lysidine synthetase; Provisional; Region:
tilS; PRK10660"
/db_xref="CDD:182626"
misc_feature 212370..212897
/gene="tilS"
/locus_tag="b0188"
/gene_synonym="ECK0187; JW0183; mesJ; yaeN"
/note="N-terminal domain of predicted ATPase of the
PP-loop faimly implicated in cell cycle control [Cell
division and chromosome partitioning]. This is a subfamily
of Adenine nucleotide alpha hydrolases
superfamily.Adeninosine nucleotide alpha hydrolases...;
Region: PP-ATPase; cd01992"
/db_xref="CDD:238950"
misc_feature order(212382..212390,212394..212405,212472..212474,
212478..212480)
/gene="tilS"
/locus_tag="b0188"
/gene_synonym="ECK0187; JW0183; mesJ; yaeN"
/note="Ligand Binding Site [chemical binding]; other site"
/db_xref="CDD:238950"
misc_feature 213051..213257
/gene="tilS"
/locus_tag="b0188"
/gene_synonym="ECK0187; JW0183; mesJ; yaeN"
/note="TilS substrate binding domain; Region: TilS;
pfam09179"
/db_xref="CDD:204159"
misc_feature 213396..213611
/gene="tilS"
/locus_tag="b0188"
/gene_synonym="ECK0187; JW0183; mesJ; yaeN"
/note="TilS substrate C-terminal domain; Region: TilS_C;
pfam11734"
/db_xref="CDD:221192"
repeat_region 213632..213668
/note="REP15 (repetitive extragenic palindromic) element;
contains 1 REP sequences"
gene complement(213678..213932)
/gene="rof"
/locus_tag="b0189"
/gene_synonym="ECK0188; JW0184; mesJ; yaeO"
/db_xref="EcoGene:EG13221"
/db_xref="GeneID:944891"
CDS complement(213678..213932)
/gene="rof"
/locus_tag="b0189"
/gene_synonym="ECK0188; JW0184; mesJ; yaeO"
/experiment="N-terminus verified by Edman degradation:
PMID 10739205"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006350 - transcription"
/codon_start=1
/transl_table=11
/product="modulator of Rho-dependent transcription
termination"
/protein_id="NP_414731.2"
/db_xref="GI:90111097"
/db_xref="ASAP:ABE-0000641"
/db_xref="UniProtKB/Swiss-Prot:P0AFW8"
/db_xref="EcoGene:EG13221"
/db_xref="GeneID:944891"
/translation="MNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASDLV
SRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
misc_feature complement(213681..213932)
/gene="rof"
/locus_tag="b0189"
/gene_synonym="ECK0188; JW0184; mesJ; yaeO"
/note="Rho-binding antiterminator; Provisional; Region:
PRK11625"
/db_xref="CDD:183241"
gene complement(213925..214125)
/gene="yaeP"
/locus_tag="b4406"
/gene_synonym="ECK0189; JW0185"
/db_xref="EcoGene:EG13222"
/db_xref="GeneID:1450233"
CDS complement(213925..214125)
/gene="yaeP"
/locus_tag="b4406"
/gene_synonym="ECK0189; JW0185"
/note="conserved hypothetical protein"
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="YP_026160.1"
/db_xref="GI:49176004"
/db_xref="ASAP:ABE-0047214"
/db_xref="UniProtKB/Swiss-Prot:P0A8K5"
/db_xref="EcoGene:EG13222"
/db_xref="GeneID:1450233"
/translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
EAVREKAAYAAANLLVSDYVNE"
misc_feature complement(213928..214125)
/gene="yaeP"
/locus_tag="b4406"
/gene_synonym="ECK0189; JW0185"
/note="hypothetical protein; Provisional; Region:
PRK04964"
/db_xref="CDD:179901"
gene 214291..214836
/gene="yaeQ"
/locus_tag="b0190"
/gene_synonym="ECK0190; JW0186"
/db_xref="EcoGene:EG13223"
/db_xref="GeneID:946809"
CDS 214291..214836
/gene="yaeQ"
/locus_tag="b0190"
/gene_synonym="ECK0190; JW0186"
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="NP_414732.1"
/db_xref="GI:16128183"
/db_xref="ASAP:ABE-0000648"
/db_xref="UniProtKB/Swiss-Prot:P0AA97"
/db_xref="EcoGene:EG13223"
/db_xref="GeneID:946809"
/translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
IWLSDDKNNLEVNLTAWQQPS"
misc_feature 214291..214833
/gene="yaeQ"
/locus_tag="b0190"
/gene_synonym="ECK0190; JW0186"
/note="Uncharacterized protein conserved in bacteria
[Function unknown]; Region: COG4681"
/db_xref="CDD:227026"
gene 214833..215255
/gene="arfB"
/locus_tag="b0191"
/gene_synonym="ECK0191; JW0187; yaeJ"
/db_xref="EcoGene:EG12354"
/db_xref="GeneID:946046"
CDS 214833..215255
/gene="arfB"
/locus_tag="b0191"
/gene_synonym="ECK0191; JW0187; yaeJ"
/codon_start=1
/transl_table=11
/product="alternative stalled-ribosome rescue factor B;
peptidyl-tRNA hydrolase, ribosome-attached"
/protein_id="NP_414733.1"
/db_xref="GI:16128184"
/db_xref="ASAP:ABE-0000650"
/db_xref="UniProtKB/Swiss-Prot:P40711"
/db_xref="EcoGene:EG12354"
/db_xref="GeneID:946046"
/translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAMIKELTTEK
KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
misc_feature 214833..215240
/gene="arfB"
/locus_tag="b0191"
/gene_synonym="ECK0191; JW0187; yaeJ"
/note="hypothetical protein; Provisional; Region:
PRK09256"
/db_xref="CDD:181730"
misc_feature <214836..215252
/gene="arfB"
/locus_tag="b0191"
/gene_synonym="ECK0191; JW0187; yaeJ"
/note="Protein chain release factor B [Translation,
ribosomal structure and biogenesis]; Region: PrfB;
COG1186"
/db_xref="CDD:224107"
gene 215269..215979
/gene="nlpE"
/locus_tag="b0192"
/gene_synonym="cutF; ECK0192; JW0188"
/db_xref="EcoGene:EG12137"
/db_xref="GeneID:946782"
CDS 215269..215979
/gene="nlpE"
/locus_tag="b0192"
/gene_synonym="cutF; ECK0192; JW0188"
/function="putative transport; Detoxification"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0009279 - cell outer membrane"
/GO_process="GO:0006805 - xenobiotic metabolic process"
/note="copper homeostasis protein (lipoprotein)"
/codon_start=1
/transl_table=11
/product="lipoprotein involved with copper homeostasis and
adhesion"
/protein_id="NP_414734.1"
/db_xref="GI:16128185"
/db_xref="ASAP:ABE-0000652"
/db_xref="UniProtKB/Swiss-Prot:P40710"
/db_xref="EcoGene:EG12137"
/db_xref="GeneID:946782"
/translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEAAQSSLPMTPMTLRGMYFYMADAA
TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEGNPDTGAPTKVLA
PDTAGKFYPNQDCSSLGQ"
misc_feature 215269..215967
/gene="nlpE"
/locus_tag="b0192"
/gene_synonym="cutF; ECK0192; JW0188"
/note="lipoprotein involved with copper homeostasis and
adhesion; Provisional; Region: PRK10523"
/db_xref="CDD:236708"
misc_feature 215401..215658
/gene="nlpE"
/locus_tag="b0192"
/gene_synonym="cutF; ECK0192; JW0188"
/note="NlpE N-terminal domain; Region: NlpE; pfam04170"
/db_xref="CDD:190894"
repeat_region 216027..216137
/note="RIP16 (repetitive extragenic palindromic) element;
contains 2 REP sequences and 1 IHF site"
gene complement(216179..217003)
/gene="yaeF"
/locus_tag="b0193"
/gene_synonym="ECK0193; JW5016; yaeK"
/db_xref="EcoGene:EG12138"
/db_xref="GeneID:944892"
CDS complement(216179..217003)
/gene="yaeF"
/locus_tag="b0193"
/gene_synonym="ECK0193; JW5016; yaeK"
/codon_start=1
/transl_table=11
/product="putative lipoprotein"
/protein_id="NP_414735.4"
/db_xref="GI:90111098"
/db_xref="ASAP:ABE-0000655"
/db_xref="UniProtKB/Swiss-Prot:P37056"
/db_xref="EcoGene:EG12138"
/db_xref="GeneID:944892"
/translation="MDKPKAYCRLFLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
misc_feature complement(216182..217003)
/gene="yaeF"
/locus_tag="b0193"
/gene_synonym="ECK0193; JW5016; yaeK"
/note="hypothetical protein; Provisional; Region:
PRK11479"
/db_xref="CDD:183157"
misc_feature complement(<216350..216814)
/gene="yaeF"
/locus_tag="b0193"
/gene_synonym="ECK0193; JW5016; yaeK"
/note="Orthopoxvirus protein of unknown function (DUF830);
Region: DUF830; cl13998"
/db_xref="CDD:246599"
gene complement(217057..218775)
/gene="proS"
/locus_tag="b0194"
/gene_synonym="drpA; ECK0194; JW0190"
/db_xref="EcoGene:EG10770"
/db_xref="GeneID:949116"
CDS complement(217057..218775)
/gene="proS"
/locus_tag="b0194"
/gene_synonym="drpA; ECK0194; JW0190"
/EC_number="6.1.1.15"
/function="enzyme; Aminoacyl tRNA synthetases, tRNA
modification"
/experiment="N-terminus verified by Edman degradation:
PMID 2203971,9298646"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006418 - tRNA aminoacylation for protein
translation"
/note="proline tRNA synthetase"
/codon_start=1
/transl_table=11
/product="prolyl-tRNA synthetase"
/protein_id="NP_414736.1"
/db_xref="GI:16128187"
/db_xref="ASAP:ABE-0000657"
/db_xref="UniProtKB/Swiss-Prot:P16659"
/db_xref="EcoGene:EG10770"
/db_xref="GeneID:949116"
/translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
TVKTLLVKAVEGSSFPQVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
GDPSPDGQGRLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
VKQIKG"
misc_feature complement(217063..218775)
/gene="proS"
/locus_tag="b0194"
/gene_synonym="drpA; ECK0194; JW0190"
/note="prolyl-tRNA synthetase; Provisional; Region:
PRK09194"
/db_xref="CDD:236405"
misc_feature complement(<218131..218727)
/gene="proS"
/locus_tag="b0194"
/gene_synonym="drpA; ECK0194; JW0190"
/note="Prolyl-tRNA synthetase (ProRS) class II core
catalytic domain. ProRS is a homodimer. It is responsible
for the attachment of proline to the 3' OH group of ribose
of the appropriate tRNA. This domain is primarily
responsible for ATP-dependent...; Region: ProRS_core_prok;
cd00779"
/db_xref="CDD:238402"
misc_feature complement(order(218314..218316,218365..218367,
218425..218427,218479..218493,218497..218499,
218557..218562,218566..218574,218623..218625,
218644..218655,218674..218679))
/gene="proS"
/locus_tag="b0194"
/gene_synonym="drpA; ECK0194; JW0190"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:238402"
misc_feature complement(218557..218580)
/gene="proS"
/locus_tag="b0194"
/gene_synonym="drpA; ECK0194; JW0190"
/note="motif 1; other site"
/db_xref="CDD:238402"
misc_feature complement(order(218299..218301,218305..218307,
218311..218313,218320..218328,218350..218352,
218356..218358,218443..218445,218449..218451))
/gene="proS"
/locus_tag="b0194"
/gene_synonym="drpA; ECK0194; JW0190"
/note="active site"
/db_xref="CDD:238402"
misc_feature complement(218350..218361)
/gene="proS"
/locus_tag="b0194"
/gene_synonym="drpA; ECK0194; JW0190"
/note="motif 2; other site"
/db_xref="CDD:238402"
misc_feature complement(217615..218100)
/gene="proS"
/locus_tag="b0194"
/gene_synonym="drpA; ECK0194; JW0190"
/note="INS is an amino acid-editing domain inserted (INS)
into the bacterial class II prolyl-tRNA synthetase (ProRS)
however, this CD is not exclusively bacterial. It is also
found at the N-terminus of the eukaryotic/archaea-like
ProRS's of yeasts and...; Region: ProRS-INS; cd04334"
/db_xref="CDD:239826"
misc_feature complement(order(217690..217692,217765..217770,
217939..217941))
/gene="proS"
/locus_tag="b0194"
/gene_synonym="drpA; ECK0194; JW0190"
/note="putative deacylase active site [active]"
/db_xref="CDD:239826"
misc_feature complement(217402..>217569)
/gene="proS"
/locus_tag="b0194"
/gene_synonym="drpA; ECK0194; JW0190"
/note="Class II tRNA amino-acyl synthetase-like catalytic
core domain. Class II amino acyl-tRNA synthetases (aaRS)
share a common fold and generally attach an amino acid to
the 3' OH of ribose of the appropriate tRNA. PheRS is an
exception in that it...; Region: class_II_aaRS-like_core;
cl00268"
/db_xref="CDD:241739"
misc_feature complement(order(217426..217428,217435..217440,
217444..217449,217537..217539,217549..217554))
/gene="proS"
/locus_tag="b0194"
/gene_synonym="drpA; ECK0194; JW0190"
/note="active site"
/db_xref="CDD:238391"
misc_feature complement(order(217426..217428,217435..217437))
/gene="proS"
/locus_tag="b0194"
/gene_synonym="drpA; ECK0194; JW0190"
/note="motif 3; other site"
/db_xref="CDD:238391"
misc_feature complement(217075..217359)
/gene="proS"
/locus_tag="b0194"
/gene_synonym="drpA; ECK0194; JW0190"
/note="ProRS Prolyl-anticodon binding domain, short
version found predominantly in bacteria. ProRS belongs to
class II aminoacyl-tRNA synthetases (aaRS). This alignment
contains the anticodon binding domain, which is
responsible for specificity in tRNA-binding; Region:
ProRS_anticodon_short; cd00861"
/db_xref="CDD:238438"
misc_feature complement(order(217135..217137,217141..217143,
217171..217173,217195..217197,217213..217215,
217330..217335))
/gene="proS"
/locus_tag="b0194"
/gene_synonym="drpA; ECK0194; JW0190"
/note="anticodon binding site; other site"
/db_xref="CDD:238438"
gene complement(218887..219594)
/gene="tsaA"
/locus_tag="b0195"
/gene_synonym="ECK0195; JW0191; yaeB"
/db_xref="EcoGene:EG11503"
/db_xref="GeneID:949112"
CDS complement(218887..219594)
/gene="tsaA"
/locus_tag="b0195"
/gene_synonym="ECK0195; JW0191; yaeB"
/function="enzyme; tRNA modification"
/codon_start=1
/transl_table=11
/product="tRNA-Thr(GGU) m(6)t(6)A37 methyltransferase,
SAM-dependent"
/protein_id="NP_414737.1"
/db_xref="GI:16128188"
/db_xref="ASAP:ABE-0000661"
/db_xref="UniProtKB/Swiss-Prot:P28634"
/db_xref="EcoGene:EG11503"
/db_xref="GeneID:949112"
/translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
RWRVTDAGFEVFALEPR"
misc_feature complement(219181..219570)
/gene="tsaA"
/locus_tag="b0195"
/gene_synonym="ECK0195; JW0191; yaeB"
/note="Escherichia coli YaeB and related proteins; Region:
UPF0066; cd09281"
/db_xref="CDD:187753"
misc_feature complement(order(219181..219198,219295..219297,
219301..219303,219307..219309,219319..219336,
219409..219411,219421..219423,219442..219444,
219448..219456,219568..219570))
/gene="tsaA"
/locus_tag="b0195"
/gene_synonym="ECK0195; JW0191; yaeB"
/note="homodimer interaction site [polypeptide binding];
other site"
/db_xref="CDD:187753"
misc_feature complement(order(219205..219207,219220..219222,
219289..219291,219295..219297,219319..219321,
219403..219408,219412..219414))
/gene="tsaA"
/locus_tag="b0195"
/gene_synonym="ECK0195; JW0191; yaeB"
/note="cofactor binding site; other site"
/db_xref="CDD:187753"
gene complement(219591..219995)
/gene="rcsF"
/locus_tag="b0196"
/gene_synonym="ECK0196; JW0192"
/db_xref="EcoGene:EG11502"
/db_xref="GeneID:949113"
CDS complement(219591..219995)
/gene="rcsF"
/locus_tag="b0196"
/gene_synonym="ECK0196; JW0192"
/function="regulator; Surface polysaccharides & antigens"
/GO_function="GO:0042280 - cell surface antigen activity,
host-interacting"
/GO_process="GO:0009242 - colanic acid biosynthetic
process"
/note="regulator in colanic acid synthesis; interacts with
RcsB"
/codon_start=1
/transl_table=11
/product="putative outer membrane protein, signal"
/protein_id="NP_414738.1"
/db_xref="GI:16128189"
/db_xref="ASAP:ABE-0000663"
/db_xref="UniProtKB/Swiss-Prot:P69411"
/db_xref="EcoGene:EG11502"
/db_xref="GeneID:949113"
/translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
misc_feature complement(219597..219995)
/gene="rcsF"
/locus_tag="b0196"
/gene_synonym="ECK0196; JW0192"
/note="outer membrane lipoprotein; Reviewed; Region: rcsF;
PRK10781"
/db_xref="CDD:182725"
gene complement(220113..220928)
/gene="metQ"
/locus_tag="b0197"
/gene_synonym="ECK0197; JW0193; metD; yaeC"
/db_xref="EcoGene:EG11504"
/db_xref="GeneID:944893"
CDS complement(220113..220928)
/gene="metQ"
/locus_tag="b0197"
/gene_synonym="ECK0197; JW0193; metD; yaeC"
/function="transport; Transport of small molecules: Amino
acids, amines"
/function="4.3.A.1.p transport; Primary Active
Transporters; Pyrophosphate Bond (ATP, GTP, P2)
Hydrolysis-driven Active Transporters; The ATP-binding
Cassette (ABC) Superfamily + ABC-type Uptake Permeases;
ABC superfamily, periplasmic binding component"
/function="6.1 cell structure; membrane"
/function="7.2 location of gene products; periplasmic
space"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0042597 - periplasmic space"
/note="D-methionine transport protein (ABC superfamily,
peri_bind)"
/codon_start=1
/transl_table=11
/product="DL-methionine transporter subunit"
/protein_id="NP_414739.1"
/db_xref="GI:16128190"
/db_xref="ASAP:ABE-0000666"
/db_xref="UniProtKB/Swiss-Prot:P28635"
/db_xref="EcoGene:EG11504"
/db_xref="GeneID:944893"
/translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
misc_feature complement(220116..220928)
/gene="metQ"
/locus_tag="b0197"
/gene_synonym="ECK0197; JW0193; metD; yaeC"
/note="DL-methionine transporter substrate-binding
subunit; Provisional; Region: metQ; PRK11063"
/db_xref="CDD:182939"
misc_feature complement(220116..220889)
/gene="metQ"
/locus_tag="b0197"
/gene_synonym="ECK0197; JW0193; metD; yaeC"
/note="lipoprotein, YaeC family; Region: TIGR00363"
/db_xref="CDD:129460"
gene complement(220968..221621)
/gene="metI"
/locus_tag="b0198"
/gene_synonym="ECK0198; JW0194; metD; yaeE"
/db_xref="EcoGene:EG11737"
/db_xref="GeneID:944894"
CDS complement(220968..221621)
/gene="metI"
/locus_tag="b0198"
/gene_synonym="ECK0198; JW0194; metD; yaeE"
/function="transport; Transport of small molecules: Amino
acids, amines"
/function="4.3.A.1.m transport; Primary Active
Transporters; Pyrophosphate Bond (ATP, GTP, P2)
Hydrolysis-driven Active Transporters; The ATP-binding
Cassette (ABC) Superfamily + ABC-type Uptake Permeases;
ABC superfamily, membrane component"
/function="6.1 cell structure; membrane"
/function="7.3 location of gene products; inner membrane"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/note="D- and L-methionine transport protein (ABC
superfamily, membrane)"
/codon_start=1
/transl_table=11
/product="DL-methionine transporter subunit"
/protein_id="NP_414740.1"
/db_xref="GI:16128191"
/db_xref="ASAP:ABE-0000668"
/db_xref="UniProtKB/Swiss-Prot:P31547"
/db_xref="EcoGene:EG11737"
/db_xref="GeneID:944894"
/translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
misc_feature complement(<221151..221582)
/gene="metI"
/locus_tag="b0198"
/gene_synonym="ECK0198; JW0194; metD; yaeE"
/note="Transmembrane subunit (TM) found in Periplasmic
Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC)
transporters which generally bind type 2 PBPs. These types
of transporters consist of a PBP, two TMs, and two
cytoplasmic ABC ATPase subunits, and...; Region: TM_PBP2;
cd06261"
/db_xref="CDD:119394"
misc_feature complement(order(221166..221168,221175..221180,
221220..221222,221271..221273,221280..221285,
221295..221297,221301..221306,221313..221315,
221319..221321,221325..221330,221394..221396,
221400..221405,221412..221441,221445..221456,
221505..221507,221520..221525,221532..221537))
/gene="metI"
/locus_tag="b0198"
/gene_synonym="ECK0198; JW0194; metD; yaeE"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:119394"
misc_feature complement(221394..221438)
/gene="metI"
/locus_tag="b0198"
/gene_synonym="ECK0198; JW0194; metD; yaeE"
/note="conserved gate region; other site"
/db_xref="CDD:119394"
misc_feature complement(order(221199..221201,221211..221216,
221232..221270))
/gene="metI"
/locus_tag="b0198"
/gene_synonym="ECK0198; JW0194; metD; yaeE"
/note="ABC-ATPase subunit interface; other site"
/db_xref="CDD:119394"
gene complement(221614..222645)
/gene="metN"
/locus_tag="b0199"
/gene_synonym="abc; ECK0199; JW0195; metD"
/db_xref="EcoGene:EG11621"
/db_xref="GeneID:944896"
CDS complement(221614..222645)
/gene="metN"
/locus_tag="b0199"
/gene_synonym="abc; ECK0199; JW0195; metD"
/function="transport; Transport of small molecules: Amino
acids, amines"
/function="4.3.A.1.a transport; Primary Active
Transporters; Pyrophosphate Bond (ATP, GTP, P2)
Hydrolysis-driven Active Transporters; The ATP-binding
Cassette (ABC) Superfamily + ABC-type Uptake Permeases;
ABC superfamily ATP binding cytoplasmic component"
/function="7.1 location of gene products; cytoplasm"
/GO_component="GO:0005737 - cytoplasm"
/note="D- and L-methionine transport protein (ABC
superfamily, atp_bind)"
/codon_start=1
/transl_table=11
/product="DL-methionine transporter subunit"
/protein_id="NP_414741.1"
/db_xref="GI:16128192"
/db_xref="ASAP:ABE-0000671"
/db_xref="UniProtKB/Swiss-Prot:P30750"
/db_xref="EcoGene:EG11621"
/db_xref="GeneID:944896"
/translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
VALPLELDNTPKDEVKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
VKVEVLGYV"
misc_feature complement(221617..222645)
/gene="metN"
/locus_tag="b0199"
/gene_synonym="abc; ECK0199; JW0195; metD"
/note="DL-methionine transporter ATP-binding subunit;
Provisional; Region: metN; PRK11153"
/db_xref="CDD:236863"
misc_feature complement(221947..222645)
/gene="metN"
/locus_tag="b0199"
/gene_synonym="abc; ECK0199; JW0195; metD"
/note="ATP-binding cassette domain of methionine
transporter; Region: ABC_MetN_methionine_transporter;
cd03258"
/db_xref="CDD:213225"
misc_feature complement(222511..222534)
/gene="metN"
/locus_tag="b0199"
/gene_synonym="abc; ECK0199; JW0195; metD"
/note="Walker A/P-loop; other site"
/db_xref="CDD:213225"
misc_feature complement(order(222049..222051,222148..222153,
222379..222381,222508..222516,222520..222525))
/gene="metN"
/locus_tag="b0199"
/gene_synonym="abc; ECK0199; JW0195; metD"
/note="ATP binding site [chemical binding]; other site"
/db_xref="CDD:213225"
misc_feature complement(222379..222390)
/gene="metN"
/locus_tag="b0199"
/gene_synonym="abc; ECK0199; JW0195; metD"
/note="Q-loop/lid; other site"
/db_xref="CDD:213225"
misc_feature complement(222196..222225)
/gene="metN"
/locus_tag="b0199"
/gene_synonym="abc; ECK0199; JW0195; metD"
/note="ABC transporter signature motif; other site"
/db_xref="CDD:213225"
misc_feature complement(222148..222165)
/gene="metN"
/locus_tag="b0199"
/gene_synonym="abc; ECK0199; JW0195; metD"
/note="Walker B; other site"
/db_xref="CDD:213225"
misc_feature complement(222130..222141)
/gene="metN"
/locus_tag="b0199"
/gene_synonym="abc; ECK0199; JW0195; metD"
/note="D-loop; other site"
/db_xref="CDD:213225"
misc_feature complement(222043..222063)
/gene="metN"
/locus_tag="b0199"
/gene_synonym="abc; ECK0199; JW0195; metD"
/note="H-loop/switch region; other site"
/db_xref="CDD:213225"
misc_feature complement(221626..221847)
/gene="metN"
/locus_tag="b0199"
/gene_synonym="abc; ECK0199; JW0195; metD"
/note="This domain is found at the C-terminus of ABC
transporter proteins involved in D-methionine transport as
well as a number of ferredoxin-like proteins; Region: NIL;
smart00930"
/db_xref="CDD:197998"
gene 222833..223408
/gene="gmhB"
/locus_tag="b0200"
/gene_synonym="ECK0200; gmbC; gmbX; gmhX; JW0196; wcbN;
yaeD"
/db_xref="EcoGene:EG11736"
/db_xref="GeneID:944879"
CDS 222833..223408
/gene="gmhB"
/locus_tag="b0200"
/gene_synonym="ECK0200; gmbC; gmbX; gmhX; JW0196; wcbN;
yaeD"
/function="enzyme; Surface polysaccharides and antigens"
/codon_start=1
/transl_table=11
/product="D,D-heptose 1,7-bisphosphate phosphatase"
/protein_id="NP_414742.1"
/db_xref="GI:16128193"
/db_xref="ASAP:ABE-0000675"
/db_xref="UniProtKB/Swiss-Prot:P63228"
/db_xref="EcoGene:EG11736"
/db_xref="GeneID:944879"
/translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
misc_feature 222845..223369
/gene="gmhB"
/locus_tag="b0200"
/gene_synonym="ECK0200; gmbC; gmbX; gmhX; JW0196; wcbN;
yaeD"
/note="D,D-heptose 1,7-bisphosphate phosphatase; Region:
GmhB_yaeD; TIGR00213"
/db_xref="CDD:129317"
misc_feature 222851..223297
/gene="gmhB"
/locus_tag="b0200"
/gene_synonym="ECK0200; gmbC; gmbX; gmhX; JW0196; wcbN;
yaeD"
/note="Haloacid dehalogenase-like hydrolases. The haloacid
dehalogenase-like (HAD) superfamily includes L-2-haloacid
dehalogenase, epoxide hydrolase, phosphoserine
phosphatase, phosphomannomutase, phosphoglycolate
phosphatase, P-type ATPase, and many others; Region:
HAD_like; cd01427"
/db_xref="CDD:119389"
misc_feature order(222863..222871,222989..222994)
/gene="gmhB"
/locus_tag="b0200"
/gene_synonym="ECK0200; gmbC; gmbX; gmhX; JW0196; wcbN;
yaeD"
/note="active site"
/db_xref="CDD:119389"
misc_feature 222863..222880
/gene="gmhB"
/locus_tag="b0200"
/gene_synonym="ECK0200; gmbC; gmbX; gmhX; JW0196; wcbN;
yaeD"
/note="motif I; other site"
/db_xref="CDD:119389"
misc_feature 222989..222991
/gene="gmhB"
/locus_tag="b0200"
/gene_synonym="ECK0200; gmbC; gmbX; gmhX; JW0196; wcbN;
yaeD"
/note="motif II; other site"
/db_xref="CDD:119389"
gene 223771..225312
/gene="rrsH"
/locus_tag="b0201"
/gene_synonym="ECK0201; JWR0001"
/db_xref="EcoGene:EG30090"
/db_xref="GeneID:944897"
rRNA 223771..225312
/gene="rrsH"
/locus_tag="b0201"
/gene_synonym="ECK0201; JWR0001"
/product="16S ribosomal RNA of rrnH operon"
/db_xref="ASAP:ABE-0000677"
/db_xref="EcoGene:EG30090"
/db_xref="GeneID:944897"
gene 225381..225457
/gene="ileV"
/locus_tag="b0202"
/gene_synonym="ECK0202; JWR0002"
/db_xref="EcoGene:EG30045"
/db_xref="GeneID:944884"
tRNA 225381..225457
/gene="ileV"
/locus_tag="b0202"
/gene_synonym="ECK0202; JWR0002"
/product="tRNA-Ile"
/note="anticodon: GAU"
/db_xref="ASAP:ABE-0000679"
/db_xref="EcoGene:EG30045"
/db_xref="GeneID:944884"
gene 225500..225575
/gene="alaV"
/locus_tag="b0203"
/gene_synonym="ECK0203; JWR0003"
/db_xref="EcoGene:EG30010"
/db_xref="GeneID:944890"
tRNA 225500..225575
/gene="alaV"
/locus_tag="b0203"
/gene_synonym="ECK0203; JWR0003"
/product="tRNA-Ala"
/note="anticodon: UGC"
/db_xref="ASAP:ABE-0000682"
/db_xref="EcoGene:EG30010"
/db_xref="GeneID:944890"
gene 225759..228662
/gene="rrlH"
/locus_tag="b0204"
/gene_synonym="ECK0204; JWR0004"
/db_xref="EcoGene:EG30083"
/db_xref="GeneID:944900"
rRNA 225759..228662
/gene="rrlH"
/locus_tag="b0204"
/gene_synonym="ECK0204; JWR0004"
/product="23S ribosomal RNA of rrnH operon"
/db_xref="ASAP:ABE-0000684"
/db_xref="EcoGene:EG30083"
/db_xref="GeneID:944900"
gene 228756..228875
/gene="rrfH"
/locus_tag="b0205"
/gene_synonym="ECK0205; JWR0005"
/db_xref="EcoGene:EG30076"
/db_xref="GeneID:944898"
rRNA 228756..228875
/gene="rrfH"
/locus_tag="b0205"
/gene_synonym="ECK0205; JWR0005"
/product="5S ribosomal RNA of rrnH operon"
/db_xref="ASAP:ABE-0000686"
/db_xref="EcoGene:EG30076"
/db_xref="GeneID:944898"
gene 228928..229004
/gene="aspU"
/locus_tag="b0206"
/gene_synonym="ECK0206; JWR0006"
/db_xref="EcoGene:EG30024"
/db_xref="GeneID:944880"
tRNA 228928..229004
/gene="aspU"
/locus_tag="b0206"
/gene_synonym="ECK0206; JWR0006"
/product="tRNA-Asp"
/note="anticodon: GUC"
/db_xref="ASAP:ABE-0000688"
/db_xref="EcoGene:EG30024"
/db_xref="GeneID:944880"
gene 229167..229970
/gene="dkgB"
/locus_tag="b0207"
/gene_synonym="ECK0207; JW0197; yafB"
/db_xref="EcoGene:EG11648"
/db_xref="GeneID:944901"
CDS 229167..229970
/gene="dkgB"
/locus_tag="b0207"
/gene_synonym="ECK0207; JW0197; yafB"
/EC_number="1.1.1.274"
/function="putative enzyme; Not classified"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0016052 - carbohydrate catabolic process"
/codon_start=1
/transl_table=11
/product="2,5-diketo-D-gluconate reductase B"
/protein_id="NP_414743.1"
/db_xref="GI:16128194"
/db_xref="ASAP:ABE-0000692"
/db_xref="UniProtKB/Swiss-Prot:P30863"
/db_xref="EcoGene:EG11648"
/db_xref="GeneID:944901"
/translation="MAIPAFGLGTFRLKDDVVISSVITALELGYRAIDTAQIYDNEAA
VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
misc_feature 229173..229916
/gene="dkgB"
/locus_tag="b0207"
/gene_synonym="ECK0207; JW0197; yafB"
/note="Aldo-keto reductases (AKRs) are a superfamily of
soluble NAD(P)(H) oxidoreductases whose chief purpose is
to reduce aldehydes and ketones to primary and secondary
alcohols. AKRs are present in all phyla and are of
importance to both health and industrial...; Region:
Aldo_ket_red; cd06660"
/db_xref="CDD:119408"
misc_feature 229179..229919
/gene="dkgB"
/locus_tag="b0207"
/gene_synonym="ECK0207; JW0197; yafB"
/note="Aldo/keto reductase family; Region: Aldo_ket_red;
pfam00248"
/db_xref="CDD:215817"
misc_feature order(229191..229199,229266..229268,229281..229283,
229356..229358,229455..229460,229551..229556,
229620..229622,229698..229715,229776..229778,
229821..229832,229845..229847,229854..229859)
/gene="dkgB"
/locus_tag="b0207"
/gene_synonym="ECK0207; JW0197; yafB"
/note="active site"
/db_xref="CDD:119408"
misc_feature order(229266..229268,229281..229283,229356..229358,
229455..229457)
/gene="dkgB"
/locus_tag="b0207"
/gene_synonym="ECK0207; JW0197; yafB"
/note="catalytic tetrad [active]"
/db_xref="CDD:119408"
gene complement(229967..230881)
/gene="yafC"
/locus_tag="b0208"
/gene_synonym="ECK0208; JW0198"
/db_xref="EcoGene:EG11649"
/db_xref="GeneID:947507"
CDS complement(229967..230881)
/gene="yafC"
/locus_tag="b0208"
/gene_synonym="ECK0208; JW0198"
/function="putative transport; Not classified"
/GO_function="GO:0016563 - transcription activator
activity"
/GO_function="GO:0016564 - transcription repressor
activity"
/GO_process="GO:0006350 - transcription"
/note="putative transcriptional regulator LYSR-type"
/codon_start=1
/transl_table=11
/product="putative DNA-binding transcriptional regulator"
/protein_id="NP_414744.1"
/db_xref="GI:16128195"
/db_xref="ASAP:ABE-0000694"
/db_xref="UniProtKB/Swiss-Prot:P30864"
/db_xref="EcoGene:EG11649"
/db_xref="GeneID:947507"
/translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
AVSTRIRAFIDFLSEHVKTAPGGAVREA"
misc_feature complement(230000..230866)
/gene="yafC"
/locus_tag="b0208"
/gene_synonym="ECK0208; JW0198"
/note="Transcriptional regulator [Transcription]; Region:
LysR; COG0583"
/db_xref="CDD:223656"
misc_feature complement(230690..230860)
/gene="yafC"
/locus_tag="b0208"
/gene_synonym="ECK0208; JW0198"
/note="Bacterial regulatory helix-turn-helix protein, lysR
family; Region: HTH_1; pfam00126"
/db_xref="CDD:215735"
misc_feature complement(230015..230611)
/gene="yafC"
/locus_tag="b0208"
/gene_synonym="ECK0208; JW0198"
/note="The C-terminal substrate binding domain of
LysR-type transcriptional regulator CrgA, contains the
type 2 periplasmic binding domain; Region: PBP2_CrgA;
cd08478"
/db_xref="CDD:176167"
misc_feature complement(order(230078..230080,230162..230164,
230213..230215,230396..230398,230402..230404,
230444..230446,230561..230563,230573..230575))
/gene="yafC"
/locus_tag="b0208"
/gene_synonym="ECK0208; JW0198"
/note="putative effector binding pocket; other site"
/db_xref="CDD:176167"
misc_feature complement(order(230186..230188,230195..230200,
230219..230233,230315..230317,230498..230518,
230522..230524,230534..230536,230543..230548,
230552..230557,230567..230572))
/gene="yafC"
/locus_tag="b0208"
/gene_synonym="ECK0208; JW0198"
/note="dimerization interface [polypeptide binding]; other
site"
/db_xref="CDD:176167"
gene 231122..231922
/gene="yafD"
/locus_tag="b0209"
/gene_synonym="ECK0209; JW5017"
/db_xref="EcoGene:EG11650"
/db_xref="GeneID:946286"
CDS 231122..231922
/gene="yafD"
/locus_tag="b0209"
/gene_synonym="ECK0209; JW5017"
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="NP_414745.1"
/db_xref="GI:16128196"
/db_xref="ASAP:ABE-0000700"
/db_xref="UniProtKB/Swiss-Prot:P0A8U2"
/db_xref="EcoGene:EG11650"
/db_xref="GeneID:946286"
/translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
misc_feature 231122..231910
/gene="yafD"
/locus_tag="b0209"
/gene_synonym="ECK0209; JW5017"
/note="hypothetical protein; Provisional; Region:
PRK05421"
/db_xref="CDD:235454"
misc_feature order(231269..231271,231350..231352,231587..231589,
231689..231691,231695..231697)
/gene="yafD"
/locus_tag="b0209"
/gene_synonym="ECK0209; JW5017"
/note="putative catalytic site [active]"
/db_xref="CDD:197306"
misc_feature 231350..231352
/gene="yafD"
/locus_tag="b0209"
/gene_synonym="ECK0209; JW5017"
/note="putative metal binding site [ion binding]; other
site"
/db_xref="CDD:197306"
misc_feature order(231587..231589,231695..231697)
/gene="yafD"
/locus_tag="b0209"
/gene_synonym="ECK0209; JW5017"
/note="putative phosphate binding site [ion binding];
other site"
/db_xref="CDD:197306"
misc_feature order(231689..231691,231695..231697,231809..231811,
231872..231877)
/gene="yafD"
/locus_tag="b0209"
/gene_synonym="ECK0209; JW5017"
/note="putative catalytic site [active]"
/db_xref="CDD:197306"
misc_feature order(231695..231697,231875..231877)
/gene="yafD"
/locus_tag="b0209"
/gene_synonym="ECK0209; JW5017"
/note="putative phosphate binding site [ion binding];
other site"
/db_xref="CDD:197306"
misc_feature 231872..231874
/gene="yafD"
/locus_tag="b0209"
/gene_synonym="ECK0209; JW5017"
/note="putative metal binding site [ion binding]; other
site"
/db_xref="CDD:197306"
gene 231926..232549
/gene="yafE"
/locus_tag="b0210"
/gene_synonym="ECK0210; JW0200"
/db_xref="EcoGene:EG11651"
/db_xref="GeneID:946197"
CDS 231926..232549
/gene="yafE"
/locus_tag="b0210"
/gene_synonym="ECK0210; JW0200"
/function="putative enzyme; Not classified"
/GO_process="GO:0009102 - biotin biosynthetic process"
/note="putative biotin synthesis protein"
/codon_start=1
/transl_table=11
/product="putative S-adenosyl-L-methionine-dependent
methyltransferase"
/protein_id="NP_414746.1"
/db_xref="GI:16128197"
/db_xref="ASAP:ABE-0000702"
/db_xref="UniProtKB/Swiss-Prot:P30866"
/db_xref="EcoGene:EG11651"
/db_xref="GeneID:946197"
/translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
ITTRQGYAESLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
GHPVRDIWLQTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVAR
MRTPEALVDAIRIYQQSASTEVRTYFALQNDGFFTSDIIMVDAHKAA"
misc_feature 231944..232342
/gene="yafE"
/locus_tag="b0210"
/gene_synonym="ECK0210; JW0200"
/note="Methyltransferase domain; Region: Methyltransf_31;
pfam13847"
/db_xref="CDD:222415"
misc_feature 231944..232222
/gene="yafE"
/locus_tag="b0210"
/gene_synonym="ECK0210; JW0200"
/note="S-adenosylmethionine-dependent methyltransferases
(SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes
that use S-adenosyl-L-methionine (SAM or AdoMet) as a
substrate for methyltransfer, creating the product
S-adenosyl-L-homocysteine (AdoHcy); Region: AdoMet_MTases;
cd02440"
/db_xref="CDD:100107"
misc_feature order(231944..231949,231995..232000,232073..232081,
232127..232129)
/gene="yafE"
/locus_tag="b0210"
/gene_synonym="ECK0210; JW0200"
/note="S-adenosylmethionine binding site [chemical
binding]; other site"
/db_xref="CDD:100107"
gene complement(232597..233955)
/gene="mltD"
/locus_tag="b0211"
/gene_synonym="dniR; ECK0211; JW5018; yafG"
/db_xref="EcoGene:EG10246"
/db_xref="GeneID:945694"
CDS complement(232597..233955)
/gene="mltD"
/locus_tag="b0211"
/gene_synonym="dniR; ECK0211; JW5018; yafG"
/EC_number="3.2.1.-"
/function="regulator; Energy metabolism, carbon: Anaerobic
respiration"
/GO_component="GO:0005737 - cytoplasm"
/GO_function="GO:0016563 - transcription activator
activity"
/GO_process="GO:0009061 - anaerobic respiration"
/GO_process="GO:0017004 - cytochrome complex assembly"
/GO_process="GO:0006350 - transcription"
/codon_start=1
/transl_table=11
/product="putative membrane-bound lytic murein
transglycosylase D"
/protein_id="NP_414747.1"
/db_xref="GI:16128198"
/db_xref="ASAP:ABE-0000704"
/db_xref="UniProtKB/Swiss-Prot:P0AEZ7"
/db_xref="EcoGene:EG10246"
/db_xref="GeneID:945694"
/translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
DS"
misc_feature complement(232600..233955)
/gene="mltD"
/locus_tag="b0211"
/gene_synonym="dniR; ECK0211; JW5018; yafG"
/note="membrane-bound lytic murein transglycosylase D;
Provisional; Region: mltD; PRK10783"
/db_xref="CDD:182727"
misc_feature complement(233239..233613)
/gene="mltD"
/locus_tag="b0211"
/gene_synonym="dniR; ECK0211; JW5018; yafG"
/note="Lytic Transglycosylase (LT) and Goose Egg White
Lysozyme (GEWL) domain. Members include the soluble and
insoluble membrane-bound LTs in bacteria, the LTs in
bacteriophage lambda, as well as, the eukaryotic
'goose-type' lysozymes (GEWL). LTs...; Region: LT_GEWL;
cd00254"
/db_xref="CDD:238157"
misc_feature complement(order(233371..233373,233428..233430,
233521..233523,233581..233583))
/gene="mltD"
/locus_tag="b0211"
/gene_synonym="dniR; ECK0211; JW5018; yafG"
/note="N-acetyl-D-glucosamine binding site [chemical
binding]; other site"
/db_xref="CDD:238157"
misc_feature complement(233581..233583)
/gene="mltD"
/locus_tag="b0211"
/gene_synonym="dniR; ECK0211; JW5018; yafG"
/note="catalytic residue [active]"
/db_xref="CDD:238157"
misc_feature complement(232804..232935)
/gene="mltD"
/locus_tag="b0211"
/gene_synonym="dniR; ECK0211; JW5018; yafG"
/note="Lysine Motif is a small domain involved in binding
peptidoglycan; Region: LysM; cd00118"
/db_xref="CDD:212030"
misc_feature complement(232630..232758)
/gene="mltD"
/locus_tag="b0211"
/gene_synonym="dniR; ECK0211; JW5018; yafG"
/note="Lysine Motif is a small domain involved in binding
peptidoglycan; Region: LysM; cd00118"
/db_xref="CDD:212030"
gene complement(234027..234782)
/gene="gloB"
/locus_tag="b0212"
/gene_synonym="ECK0212; JW0202; yafR"
/db_xref="EcoGene:EG13330"
/db_xref="GeneID:944902"
CDS complement(234027..234782)
/gene="gloB"
/locus_tag="b0212"
/gene_synonym="ECK0212; JW0202; yafR"
/function="putative enzyme; Central intermediary
metabolism: Pool, multipurpose conversions"
/GO_process="GO:0009438 - methylglyoxal metabolic process"
/note="probable hydroxyacylglutathione hydrolase"
/codon_start=1
/transl_table=11
/product="hydroxyacylglutathione hydrolase"
/protein_id="NP_414748.1"
/db_xref="GI:16128199"
/db_xref="ASAP:ABE-0000707"
/db_xref="UniProtKB/Swiss-Prot:P0AC84"
/db_xref="EcoGene:EG13330"
/db_xref="GeneID:944902"
/translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
misc_feature complement(234030..234782)
/gene="gloB"
/locus_tag="b0212"
/gene_synonym="ECK0212; JW0202; yafR"
/note="hydroxyacylglutathione hydrolase; Provisional;
Region: PRK10241"
/db_xref="CDD:182327"
gene 234816..235538
/gene="yafS"
/locus_tag="b0213"
/gene_synonym="ECK0213; JW0203"
/db_xref="EcoGene:EG13331"
/db_xref="GeneID:944903"
CDS 234816..235538
/gene="yafS"
/locus_tag="b0213"
/gene_synonym="ECK0213; JW0203"
/codon_start=1
/transl_table=11
/product="putative S-adenosyl-L-methionine-dependent
methyltransferase"
/protein_id="NP_414749.2"
/db_xref="GI:90111099"
/db_xref="ASAP:ABE-0000713"
/db_xref="UniProtKB/Swiss-Prot:P75672"
/db_xref="EcoGene:EG13331"
/db_xref="GeneID:944903"
/translation="MKPARVPQTVVAPDCWGDLPWGKLYRKALERQLNPWFTKMYGFH
LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
CTDPHRLLREADRVLIDDGWLVISGFNPISFMGLRKLVPVLRKTSPYNSRMFTLMRQL
DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
SKNKPRIRQAVGATRQCRKPQA"
misc_feature <234975..235193
/gene="yafS"
/locus_tag="b0213"
/gene_synonym="ECK0213; JW0203"
/note="S-adenosylmethionine-dependent methyltransferases
(SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes
that use S-adenosyl-L-methionine (SAM or AdoMet) as a
substrate for methyltransfer, creating the product
S-adenosyl-L-homocysteine (AdoHcy); Region: AdoMet_MTases;
cd02440"
/db_xref="CDD:100107"
gene complement(235535..236002)
/gene="rnhA"
/locus_tag="b0214"
/gene_synonym="cer; dasF; ECK0214; herA; JW0204; rnh;
sdrA; sin"
/db_xref="EcoGene:EG10860"
/db_xref="GeneID:946955"
CDS complement(235535..236002)
/gene="rnhA"
/locus_tag="b0214"
/gene_synonym="cer; dasF; ECK0214; herA; JW0204; rnh;
sdrA; sin"
/EC_number="3.1.26.4"
/function="enzyme; Degradation of RNA"
/experiment="N-terminus verified by Edman degradation:
PMID 2171503"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006401 - RNA catabolic process"
/GO_process="GO:0006261 - DNA-dependent DNA replication"
/GO_process="GO:0006508 - proteolysis"
/note="RNase HI, degrades RNA of DNA-RNA hybrids,
participates in DNA replication"
/codon_start=1
/transl_table=11
/product="ribonuclease HI, degrades RNA of DNA-RNA
hybrids"
/protein_id="NP_414750.1"
/db_xref="GI:16128201"
/db_xref="ASAP:ABE-0000717"
/db_xref="UniProtKB/Swiss-Prot:P0A7Y4"
/db_xref="EcoGene:EG10860"
/db_xref="GeneID:946955"
/translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
misc_feature complement(235580..235993)
/gene="rnhA"
/locus_tag="b0214"
/gene_synonym="cer; dasF; ECK0214; herA; JW0204; rnh;
sdrA; sin"
/note="RNase HI family found mainly in prokaryotes;
Region: RNase_HI_prokaryote_like; cd09278"
/db_xref="CDD:187702"
misc_feature complement(order(235589..235591,235643..235645,
235793..235795,235859..235861,235868..235876,
235964..235975))
/gene="rnhA"
/locus_tag="b0214"
/gene_synonym="cer; dasF; ECK0214; herA; JW0204; rnh;
sdrA; sin"
/note="RNA/DNA hybrid binding site [nucleotide binding];
other site"
/db_xref="CDD:187702"
misc_feature complement(order(235601..235603,235793..235795,
235859..235861,235973..235975))
/gene="rnhA"
/locus_tag="b0214"
/gene_synonym="cer; dasF; ECK0214; herA; JW0204; rnh;
sdrA; sin"
/note="active site"
/db_xref="CDD:187702"
gene 236067..236798
/gene="dnaQ"
/locus_tag="b0215"
/gene_synonym="ECK0215; JW0205; mutD"
/db_xref="EcoGene:EG10243"
/db_xref="GeneID:946441"
CDS 236067..236798
/gene="dnaQ"
/locus_tag="b0215"
/gene_synonym="ECK0215; JW0205; mutD"
/EC_number="2.7.7.7"
/function="enzyme; DNA - replication, repair,
restriction/modification"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006261 - DNA-dependent DNA replication"
/GO_process="GO:0006457 - protein folding"
/note="DNA polymerase III, epsilon subunit"
/codon_start=1
/transl_table=11
/product="DNA polymerase III epsilon subunit"
/protein_id="NP_414751.1"
/db_xref="GI:16128202"
/db_xref="ASAP:ABE-0000722"
/db_xref="UniProtKB/Swiss-Prot:P03007"
/db_xref="EcoGene:EG10243"
/db_xref="GeneID:946441"
/translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
DEEIAAHEARLDLVQKKGGSCLWRA"
misc_feature 236085..236774
/gene="dnaQ"
/locus_tag="b0215"
/gene_synonym="ECK0215; JW0205; mutD"
/note="DNA polymerase III, epsilon subunit,
Proteobacterial; Region: dnaQ_proteo; TIGR01406"
/db_xref="CDD:130473"
misc_feature 236088..236603
/gene="dnaQ"
/locus_tag="b0215"
/gene_synonym="ECK0215; JW0205; mutD"
/note="DEDDh 3'-5' exonuclease domain of the epsilon
subunit of Escherichia coli DNA polymerase III and similar
proteins; Region: DNA_pol_III_epsilon_Ecoli_like; cd06131"
/db_xref="CDD:99835"
misc_feature order(236100..236111,236115..236120,236247..236252,
236259..236264,236358..236363,236367..236375,
236496..236501,236541..236543,236550..236552,
236565..236567)
/gene="dnaQ"
/locus_tag="b0215"
/gene_synonym="ECK0215; JW0205; mutD"
/note="active site"
/db_xref="CDD:99835"
misc_feature order(236100..236111,236115..236120,236247..236252,
236259..236264,236358..236363,236367..236372,
236496..236501,236541..236543,236550..236552,
236565..236567)
/gene="dnaQ"
/locus_tag="b0215"
/gene_synonym="ECK0215; JW0205; mutD"
/note="substrate binding site [chemical binding]; other
site"
/db_xref="CDD:99835"
misc_feature order(236100..236102,236106..236108,236373..236375,
236550..236552,236565..236567)
/gene="dnaQ"
/locus_tag="b0215"
/gene_synonym="ECK0215; JW0205; mutD"
/note="catalytic site [active]"
/db_xref="CDD:99835"
gene 236931..237007
/gene="aspV"
/locus_tag="b0216"
/gene_synonym="ECK0216; JWR0007"
/db_xref="EcoGene:EG30025"
/db_xref="GeneID:947541"
tRNA 236931..237007
/gene="aspV"
/locus_tag="b0216"
/gene_synonym="ECK0216; JWR0007"
/product="tRNA-Asp"
/note="anticodon: GUC"
/db_xref="ASAP:ABE-0000724"
/db_xref="EcoGene:EG30025"
/db_xref="GeneID:947541"
gene 237335..238120
/gene="yafT"
/locus_tag="b0217"
/gene_synonym="ECK0217; JW0206"
/db_xref="EcoGene:EG13332"
/db_xref="GeneID:949009"
CDS 237335..238120
/gene="yafT"
/locus_tag="b0217"
/gene_synonym="ECK0217; JW0206"
/function="putative enzyme; Not classified"
/note="putative aminopeptidase"
/codon_start=1
/transl_table=11
/product="lipoprotein"
/protein_id="NP_414752.1"
/db_xref="GI:16128203"
/db_xref="ASAP:ABE-0000727"
/db_xref="UniProtKB/Swiss-Prot:P77339"
/db_xref="EcoGene:EG13332"
/db_xref="GeneID:949009"
/translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
YQDMLQTGKYDSALKSTVWSDYKNDKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
gene complement(238257..238736)
/gene="ykfM"
/locus_tag="b4586"
/gene_synonym="ECK4397"
/db_xref="EcoGene:EG14451"
/db_xref="GeneID:5061498"
CDS complement(238257..238736)
/gene="ykfM"
/locus_tag="b4586"
/gene_synonym="ECK4397"
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="YP_001165307.1"
/db_xref="GI:145698220"
/db_xref="UniProtKB/Swiss-Prot:P71282"
/db_xref="EcoGene:EG14451"
/db_xref="GeneID:5061498"
/translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKSFLIKKTKVVNFATDITIQLTLIFLL
IAIVIAPLIAPFVTGYVNTNYHPCGNNTGIFPGAIYIKNGMKCNNGYISRKEDSAVK"
gene complement(238746..239102)
/gene="yafU"
/locus_tag="b0218"
/gene_synonym="ECK0218; JW0207"
/pseudo
/db_xref="EcoGene:EG13333"
/db_xref="GeneID:946644"
CDS complement(238746..239102)
/gene="yafU"
/locus_tag="b0218"
/gene_synonym="ECK0218; JW0207"
/note="pseudogene"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="ASAP:ABE-0000729"
/db_xref="UniProtKB/Swiss-Prot:P77354"
/db_xref="EcoGene:EG13333"
/db_xref="GeneID:946644"
gene 239106..239378
/gene="yafF"
/locus_tag="b4503"
/gene_synonym="ECK0219; JW0208"
/pseudo
/db_xref="EcoGene:EG11772"
/db_xref="GeneID:1450235"
CDS 239106..239378
/gene="yafF"
/locus_tag="b4503"
/gene_synonym="ECK0219; JW0208"
/GO_component="GO:0005737 - cytoplasm"
/note="pseudogene, H repeat-associated protein"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="ASAP:ABE-0285024"
/db_xref="UniProtKB/Swiss-Prot:Q2EEP9"
/db_xref="EcoGene:EG11772"
/db_xref="GeneID:1450235"
gene complement(239419..240189)
/gene="yafV"
/locus_tag="b0219"
/gene_synonym="ECK0220; JW5019"
/db_xref="EcoGene:EG13334"
/db_xref="GeneID:946585"
CDS complement(239419..240189)
/gene="yafV"
/locus_tag="b0219"
/gene_synonym="ECK0220; JW5019"
/function="putative enzyme; Not classified"
/note="putative EC 3.5. amidase-type enzyme"
/codon_start=1
/transl_table=11
/product="putative C-N hydrolase family amidase,
NAD(P)-binding"
/protein_id="NP_414754.1"
/db_xref="GI:16128205"
/db_xref="ASAP:ABE-0000734"
/db_xref="UniProtKB/Swiss-Prot:Q47679"
/db_xref="EcoGene:EG13334"
/db_xref="GeneID:946585"
/translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
TADAHQATRIDAELSMAALREYREKFPAWQDADEFRLW"
misc_feature complement(239422..240189)
/gene="yafV"
/locus_tag="b0219"
/gene_synonym="ECK0220; JW5019"
/note="C-N hydrolase family amidase; Provisional; Region:
PRK10438"
/db_xref="CDD:182461"
misc_feature complement(239431..240180)
/gene="yafV"
/locus_tag="b0219"
/gene_synonym="ECK0220; JW5019"
/note="Xanthomonas campestris XC1258 and related proteins,
members of the nitrilase superfamily (putative class 13
nitrilases); Region: Xc-1258_like; cd07575"
/db_xref="CDD:143599"
misc_feature complement(order(239695..239700,239755..239760,
239764..239769,239842..239844,239857..239859,
239869..239871,240046..240048,240064..240066))
/gene="yafV"
/locus_tag="b0219"
/gene_synonym="ECK0220; JW5019"
/note="putative active site [active]"
/db_xref="CDD:143599"
misc_feature complement(order(239767..239769,239869..239871,
240064..240066))
/gene="yafV"
/locus_tag="b0219"
/gene_synonym="ECK0220; JW5019"
/note="catalytic triad [active]"
/db_xref="CDD:143599"
misc_feature complement(order(239431..239448,239452..239463,
239500..239502,239506..239511,239515..239517,
239527..239553,239557..239559,239641..239646,
239650..239658,239662..239667,239674..239679,
239737..239739,239743..239757,239764..239766,
239851..239853,239863..239868))
/gene="yafV"
/locus_tag="b0219"
/gene_synonym="ECK0220; JW5019"
/note="multimer interface [polypeptide binding]; other
site"
/db_xref="CDD:143599"
misc_feature complement(order(239431..239448,239452..239463,
239545..239553,239641..239646,239650..239658,
239662..239667,239674..239679,239737..239739,
239743..239757,239764..239766,239851..239853,
239863..239868))
/gene="yafV"
/locus_tag="b0219"
/gene_synonym="ECK0220; JW5019"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:143599"
gene 240343..240816
/gene="ivy"
/locus_tag="b0220"
/gene_synonym="ECK0221; JW0210; ykfE"
/db_xref="EcoGene:EG13547"
/db_xref="GeneID:946530"
CDS 240343..240816
/gene="ivy"
/locus_tag="b0220"
/gene_synonym="ECK0221; JW0210; ykfE"
/note="inhibitor of vertebrate C-type lysozyme,
periplasmic"
/codon_start=1
/transl_table=11
/product="inhibitor of c-type lysozyme, periplasmic"
/protein_id="NP_414755.1"
/db_xref="GI:16128206"
/db_xref="ASAP:ABE-0000740"
/db_xref="UniProtKB/Swiss-Prot:P0AD59"
/db_xref="EcoGene:EG13547"
/db_xref="GeneID:946530"
/translation="MGRISSGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
SNQMTGLFSTIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
misc_feature 240355..240813
/gene="ivy"
/locus_tag="b0220"
/gene_synonym="ECK0221; JW0210; ykfE"
/note="C-lysozyme inhibitor; Provisional; Region:
PRK09993"
/db_xref="CDD:182187"
mat_peptide 240427..240813
/gene="ivy"
/locus_tag="b0220"
/gene_synonym="ECK0221; JW0210; ykfE"
/product="inhibitor of c-type lysozyme, periplasmic"
/experiment="N-terminus verified by Edman degradation:
PMID 8740179,9868784,11278658"
gene complement(240859..243303)
/gene="fadE"
/locus_tag="b0221"
/gene_synonym="ECK0222; fadF; JW5020; yafH"
/db_xref="EcoGene:EG13145"
/db_xref="GeneID:949007"
CDS complement(240859..243303)
/gene="fadE"
/locus_tag="b0221"
/gene_synonym="ECK0222; fadF; JW5020; yafH"
/EC_number="1.3.99.-"
/function="putative enzyme; Not classified"
/GO_process="GO:0019395 - fatty acid oxidation"
/codon_start=1
/transl_table=11
/product="acyl coenzyme A dehydrogenase"
/protein_id="NP_414756.2"
/db_xref="GI:90111100"
/db_xref="ASAP:ABE-0000743"
/db_xref="UniProtKB/Swiss-Prot:Q47146"
/db_xref="EcoGene:EG13145"
/db_xref="GeneID:949007"
/translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
RICKELGKNLPFTRLDELAHNALVKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
TKPVKLPEKVRKVEAA"
misc_feature complement(240862..243180)
/gene="fadE"
/locus_tag="b0221"
/gene_synonym="ECK0222; fadF; JW5020; yafH"
/note="acyl-CoA dehydrogenase; Reviewed; Region: fadE;
PRK09463"
/db_xref="CDD:236528"
misc_feature complement(241804..242850)
/gene="fadE"
/locus_tag="b0221"
/gene_synonym="ECK0222; fadF; JW5020; yafH"
/note="Acyl-CoA dehydrogenase; Region: ACAD; cl09933"
/db_xref="CDD:245208"
misc_feature complement(order(241807..241809,241813..241821,
242461..242463,242467..242469,242584..242586,
242590..242592,242683..242685))
/gene="fadE"
/locus_tag="b0221"
/gene_synonym="ECK0222; fadF; JW5020; yafH"
/note="active site"
/db_xref="CDD:173838"
misc_feature complement(240928..241764)
/gene="fadE"
/locus_tag="b0221"
/gene_synonym="ECK0222; fadF; JW5020; yafH"
/note="Domain of unknown function (DUF1974); Region:
DUF1974; pfam09317"
/db_xref="CDD:220176"
gene 243543..244121
/gene="gmhA"
/locus_tag="b0222"
/gene_synonym="ECK0223; isn; JW0212; lpcA; tfrA; yafI"
/db_xref="EcoGene:EG13146"
/db_xref="GeneID:949134"
CDS 243543..244121
/gene="gmhA"
/locus_tag="b0222"
/gene_synonym="ECK0223; isn; JW0212; lpcA; tfrA; yafI"
/EC_number="5.-.-.-"
/function="enzyme; Surface polysaccharides & antigens"
/GO_component="GO:0005737 - cytoplasm"
/GO_function="GO:0042280 - cell surface antigen activity,
host-interacting"
/GO_process="GO:0009244 - lipopolysaccharide core region
biosynthetic process"
/note="phosphoheptose isomerase"
/codon_start=1
/transl_table=11
/product="D-sedoheptulose 7-phosphate isomerase"
/protein_id="NP_414757.1"
/db_xref="GI:16128208"
/db_xref="ASAP:ABE-0000751"
/db_xref="UniProtKB/Swiss-Prot:P63224"
/db_xref="EcoGene:EG13146"
/db_xref="GeneID:949134"
/translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
misc_feature 243570..244106
/gene="gmhA"
/locus_tag="b0222"
/gene_synonym="ECK0223; isn; JW0212; lpcA; tfrA; yafI"
/note="Phosphoheptose isomerase is a member of the SIS
(Sugar ISomerase) superfamily. Phosphoheptose isomerase
catalyzes the isomerization of sedoheptulose 7-phosphate
into D-glycero-D-mannoheptose 7-phosphate. This is the
first step of the biosynthesis of...; Region: SIS_GmhA;
cd05006"
/db_xref="CDD:240139"
misc_feature order(243579..243581,243591..243593,243633..243635,
243642..243644,243702..243704,243720..243725,
244056..244061,244071..244073,244080..244082,
244092..244094,244104..244106)
/gene="gmhA"
/locus_tag="b0222"
/gene_synonym="ECK0223; isn; JW0212; lpcA; tfrA; yafI"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:240139"
misc_feature order(243696..243704,243897..243905,243912..243914,
244041..244043,244056..244058)
/gene="gmhA"
/locus_tag="b0222"
/gene_synonym="ECK0223; isn; JW0212; lpcA; tfrA; yafI"
/note="active site"
/db_xref="CDD:240139"
repeat_region 244129..244263
/note="REP17 (repetitive extragenic palindromic) element;
contains 3 REP sequences"
gene 244327..245094
/gene="yafJ"
/locus_tag="b0223"
/gene_synonym="ECK0224; JW0213"
/db_xref="EcoGene:EG13147"
/db_xref="GeneID:944906"
CDS 244327..245094
/gene="yafJ"
/locus_tag="b0223"
/gene_synonym="ECK0224; JW0213"
/function="putative enzyme; Not classified"
/note="putative amidotransferase"
/codon_start=1
/transl_table=11
/product="putative amidotransfease"
/protein_id="NP_414758.1"
/db_xref="GI:16128209"
/db_xref="ASAP:ABE-0000755"
/db_xref="UniProtKB/Swiss-Prot:Q47147"
/db_xref="EcoGene:EG13147"
/db_xref="GeneID:944906"
/translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
misc_feature 244327..245091
/gene="yafJ"
/locus_tag="b0223"
/gene_synonym="ECK0224; JW0213"
/note="Predicted glutamine amidotransferase [General
function prediction only]; Region: COG0121"
/db_xref="CDD:223199"
misc_feature 244327..245079
/gene="yafJ"
/locus_tag="b0223"
/gene_synonym="ECK0224; JW0213"
/note="Glutamine amidotransferases class-II
(Gn-AT)_YafJ-type. YafJ is a glutamine
amidotransferase-like protein of unknown function found in
prokaryotes, eukaryotes and archaea. YafJ has a conserved
structural fold similar to those of other class II...;
Region: YafJ; cd01908"
/db_xref="CDD:238889"
misc_feature order(244330..244332,244561..244563,244648..244656,
244711..244713)
/gene="yafJ"
/locus_tag="b0223"
/gene_synonym="ECK0224; JW0213"
/note="putative active site [active]"
/db_xref="CDD:238889"
misc_feature order(244465..244467,244474..244479,244612..244614,
244684..244689,244750..244752)
/gene="yafJ"
/locus_tag="b0223"
/gene_synonym="ECK0224; JW0213"
/note="putative dimer interface [polypeptide binding];
other site"
/db_xref="CDD:238889"
gene complement(245065..245805)
/gene="yafK"
/locus_tag="b0224"
/gene_synonym="ECK0225; JW0214"
/db_xref="EcoGene:EG13148"
/db_xref="GeneID:944910"
CDS complement(245065..245805)
/gene="yafK"
/locus_tag="b0224"
/gene_synonym="ECK0225; JW0214"
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="NP_414759.1"
/db_xref="GI:16128210"
/db_xref="ASAP:ABE-0000757"
/db_xref="UniProtKB/Swiss-Prot:P0AA99"
/db_xref="EcoGene:EG13148"
/db_xref="GeneID:944910"
/translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
GRYVVSKPLSHEVVQPQLASNYTLPEAK"
misc_feature complement(245068..245805)
/gene="yafK"
/locus_tag="b0224"
/gene_synonym="ECK0225; JW0214"
/note="Uncharacterized protein conserved in bacteria
[Function unknown]; Region: COG3034"
/db_xref="CDD:225577"
misc_feature complement(245329..245679)
/gene="yafK"
/locus_tag="b0224"
/gene_synonym="ECK0225; JW0214"
/note="L,D-transpeptidase catalytic domain; Region: YkuD;
pfam03734"
/db_xref="CDD:217702"
gene complement(245961..246239)
/gene="yafQ"
/locus_tag="b0225"
/gene_synonym="ECK0226; JW0215"
/db_xref="EcoGene:EG13154"
/db_xref="GeneID:944911"
CDS complement(245961..246239)
/gene="yafQ"
/locus_tag="b0225"
/gene_synonym="ECK0226; JW0215"
/codon_start=1
/transl_table=11
/product="translation inhibitor toxin of toxin-antitoxin
pair YafQ/DinJ"
/protein_id="NP_414760.1"
/db_xref="GI:16128211"
/db_xref="ASAP:ABE-0000761"
/db_xref="UniProtKB/Swiss-Prot:Q47149"
/db_xref="EcoGene:EG13154"
/db_xref="GeneID:944911"
/translation="MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLP
AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
misc_feature complement(245967..246233)
/gene="yafQ"
/locus_tag="b0225"
/gene_synonym="ECK0226; JW0215"
/note="Uncharacterized protein conserved in bacteria
[Function unknown]; Region: COG3041"
/db_xref="CDD:225583"
gene complement(246242..246502)
/gene="dinJ"
/locus_tag="b0226"
/gene_synonym="ECK0227; JW0216; sosA"
/db_xref="EcoGene:EG13142"
/db_xref="GeneID:944914"
CDS complement(246242..246502)
/gene="dinJ"
/locus_tag="b0226"
/gene_synonym="ECK0227; JW0216; sosA"
/function="phenotype; Not classified"
/GO_process="GO:0006281 - DNA repair"
/note="damage-inducible protein J"
/codon_start=1
/transl_table=11
/product="antitoxin of YafQ-DinJ toxin-antitoxin system"
/protein_id="NP_414761.1"
/db_xref="GI:16128212"
/db_xref="ASAP:ABE-0000764"
/db_xref="UniProtKB/Swiss-Prot:Q47150"
/db_xref="EcoGene:EG13142"
/db_xref="GeneID:944914"
/translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
KALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDLFDKLGI"
misc_feature complement(246287..246493)
/gene="dinJ"
/locus_tag="b0226"
/gene_synonym="ECK0227; JW0216; sosA"
/note="RelB antitoxin; Region: RelB; pfam04221"
/db_xref="CDD:113011"
gene 246712..247461
/gene="yafL"
/locus_tag="b0227"
/gene_synonym="ECK0228; JW0217"
/db_xref="EcoGene:EG13149"
/db_xref="GeneID:944899"
CDS 246712..247461
/gene="yafL"
/locus_tag="b0227"
/gene_synonym="ECK0228; JW0217"
/function="putative membrane; Not classified"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/note="putative lipoprotein"
/codon_start=1
/transl_table=11
/product="putative lipoprotein and C40 family peptidase"
/protein_id="NP_414762.1"
/db_xref="GI:16128213"
/db_xref="ASAP:ABE-0000775"
/db_xref="UniProtKB/Swiss-Prot:Q47151"
/db_xref="EcoGene:EG13149"
/db_xref="GeneID:944899"
/translation="MSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQN
RARLLKQYQTHLKKQASYIVEGNAESKRALRQHNREQIKQHPEWFPAPLKASDRRWQA
LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
GETIRISRLAEPFWQDHFLGARRILTEETIL"
misc_feature <247012..247431
/gene="yafL"
/locus_tag="b0227"
/gene_synonym="ECK0228; JW0217"
/note="Cell wall-associated hydrolases
(invasion-associated proteins) [Cell envelope biogenesis,
outer membrane]; Region: Spr; COG0791"
/db_xref="CDD:223862"
misc_feature 247102..247434
/gene="yafL"
/locus_tag="b0227"
/gene_synonym="ECK0228; JW0217"
/note="NlpC/P60 family; Region: NLPC_P60; pfam00877"
/db_xref="CDD:189752"
repeat_region 247458..247541
/note="REP18 (repetitive extragenic palindromic) element;
contains 2 REP sequences"
gene 247637..248134
/gene="rayT"
/locus_tag="b0228"
/gene_synonym="ECK0229; JW0218; tnpAREP; yafM"
/db_xref="EcoGene:EG13150"
/db_xref="GeneID:944913"
CDS 247637..248134
/gene="rayT"
/locus_tag="b0228"
/gene_synonym="ECK0229; JW0218; tnpAREP; yafM"
/codon_start=1
/transl_table=11
/product="RAYT REP element-mobilizing transposase;
TnpA(REP)"
/protein_id="NP_414763.1"
/db_xref="GI:16128214"
/db_xref="ASAP:ABE-0000779"
/db_xref="UniProtKB/Swiss-Prot:Q47152"
/db_xref="EcoGene:EG13150"
/db_xref="GeneID:944913"
/translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRHAIIKVKR
DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDFSAG
ERIIS"
misc_feature 247637..248041
/gene="rayT"
/locus_tag="b0228"
/gene_synonym="ECK0229; JW0218; tnpAREP; yafM"
/note="Transposase and inactivated derivatives [DNA
replication, recombination, and repair]; Region: COG1943"
/db_xref="CDD:224854"
repeat_region 248147..248334
/note="REP19 (repetitive extragenic palindromic) element;
contains 4 REP sequences"
gene complement(248358..250070)
/gene="lfhA"
/locus_tag="b0229"
/gene_synonym="ECK0230; fhiA; JW5811"
/pseudo
/db_xref="EcoGene:EG13144"
/db_xref="GeneID:944908"
CDS complement(248358..250070)
/gene="lfhA"
/locus_tag="b0229"
/gene_synonym="ECK0230; fhiA; JW5811"
/GO_component="GO:0019861 - flagellum"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/note="pseudogene, flagellar system protein, promoterless
fragment;
flagellar biosynthesis"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="ASAP:ABE-0000782"
/db_xref="UniProtKB/Swiss-Prot:Q47153"
/db_xref="EcoGene:EG13144"
/db_xref="GeneID:944908"
gene 250072..250827
/gene="lafU"
/locus_tag="b0230"
/gene_synonym="ECK0231; JW5812; mbhA"
/pseudo
/db_xref="EcoGene:EG13143"
/db_xref="GeneID:944921"
CDS 250072..250827
/gene="lafU"
/locus_tag="b0230"
/gene_synonym="ECK0231; JW5812; mbhA"
/GO_process="GO:0042330 - taxis"
/note="pseudogene, lateral flagellar motor protein
fragment"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="ASAP:ABE-0000785"
/db_xref="UniProtKB/Swiss-Prot:Q47154"
/db_xref="EcoGene:EG13143"
/db_xref="GeneID:944921"
gene 250898..251953
/gene="dinB"
/locus_tag="b0231"
/gene_synonym="dinP; ECK0232; JW0221"
/db_xref="EcoGene:EG13141"
/db_xref="GeneID:944922"
CDS 250898..251953
/gene="dinB"
/locus_tag="b0231"
/gene_synonym="dinP; ECK0232; JW0221"
/EC_number="2.7.7.7"
/function="2.1.1 information transfer; DNA related; DNA
replication"
/function="5.8 cell processes; SOS response"
/function="7.1 location of gene products; cytoplasm"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006261 - DNA-dependent DNA replication"
/GO_process="GO:0009432 - SOS response"
/note="DNA polymerase IV;
DNA polymerase IV, devoid of proofreading,
damage-inducible protein P"
/codon_start=1
/transl_table=11
/product="DNA polymerase IV"
/protein_id="NP_414766.1"
/db_xref="GI:16128217"
/db_xref="ASAP:ABE-0000789"
/db_xref="UniProtKB/Swiss-Prot:Q47155"
/db_xref="EcoGene:EG13141"
/db_xref="GeneID:944922"
/translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
HVTLLDPQMERQLVLGL"
misc_feature 250910..251863
/gene="dinB"
/locus_tag="b0231"
/gene_synonym="dinP; ECK0232; JW0221"
/note="DNA Polymerase IV/Kappa; Region: PolY_Pol_IV_kappa;
cd03586"
/db_xref="CDD:176459"
misc_feature order(250919..250924,250928..250936,251021..251026,
251033..251035,251042..251044,251204..251206,
251366..251368)
/gene="dinB"
/locus_tag="b0231"
/gene_synonym="dinP; ECK0232; JW0221"
/note="active site"
/db_xref="CDD:176459"
misc_feature 250922..251950
/gene="dinB"
/locus_tag="b0231"
/gene_synonym="dinP; ECK0232; JW0221"
/note="DNA polymerase IV; Validated; Region: PRK02406"
/db_xref="CDD:235035"
misc_feature order(250994..250996,251069..251071,251198..251200,
251204..251209,251345..251347,251441..251461,
251546..251551,251618..251641,251714..251716,
251789..251800)
/gene="dinB"
/locus_tag="b0231"
/gene_synonym="dinP; ECK0232; JW0221"
/note="DNA binding site [nucleotide binding]"
/db_xref="CDD:176459"
gene 252005..252298
/gene="yafN"
/locus_tag="b0232"
/gene_synonym="ECK0233; JW0222"
/db_xref="EcoGene:EG13151"
/db_xref="GeneID:944920"
CDS 252005..252298
/gene="yafN"
/locus_tag="b0232"
/gene_synonym="ECK0233; JW0222"
/codon_start=1
/transl_table=11
/product="antitoxin of the YafO-YafN toxin-antitoxin
system"
/protein_id="NP_414767.1"
/db_xref="GI:16128218"
/db_xref="ASAP:ABE-0000793"
/db_xref="UniProtKB/Swiss-Prot:Q47156"
/db_xref="EcoGene:EG13151"
/db_xref="GeneID:944920"
/translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE"
misc_feature 252005..252295
/gene="yafN"
/locus_tag="b0232"
/gene_synonym="ECK0233; JW0222"
/note="putative antitoxin of the YafO-YafN toxin-antitoxin
system; Provisional; Region: PRK09778"
/db_xref="CDD:170091"
gene 252301..252699
/gene="yafO"
/locus_tag="b0233"
/gene_synonym="ECK0234; JW0223"
/db_xref="EcoGene:EG13152"
/db_xref="GeneID:944916"
CDS 252301..252699
/gene="yafO"
/locus_tag="b0233"
/gene_synonym="ECK0234; JW0223"
/codon_start=1
/transl_table=11
/product="mRNA interferase toxin of the YafO-YafN
toxin-antitoxin system"
/protein_id="NP_414768.1"
/db_xref="GI:16128219"
/db_xref="ASAP:ABE-0000795"
/db_xref="UniProtKB/Swiss-Prot:Q47157"
/db_xref="EcoGene:EG13152"
/db_xref="GeneID:944916"
/translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
DDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
misc_feature 252301..252696
/gene="yafO"
/locus_tag="b0233"
/gene_synonym="ECK0234; JW0223"
/note="putative toxin YafO; Provisional; Region: PRK09885"
/db_xref="CDD:236631"
gene 252709..253161
/gene="yafP"
/locus_tag="b0234"
/gene_synonym="ECK0235; JW0224"
/db_xref="EcoGene:EG13153"
/db_xref="GeneID:944912"
CDS 252709..253161
/gene="yafP"
/locus_tag="b0234"
/gene_synonym="ECK0235; JW0224"
/codon_start=1
/transl_table=11
/product="putative acyltransferase with acyl-CoA
N-acyltransferase domain"
/protein_id="NP_414769.1"
/db_xref="GI:16128220"
/db_xref="ASAP:ABE-0000801"
/db_xref="UniProtKB/Swiss-Prot:Q47158"
/db_xref="EcoGene:EG13153"
/db_xref="GeneID:944912"
/translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQISAWAQI
DESRWKEKLAKSQVWVAIINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPLIK
SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFYMRYKPQH"
misc_feature 252745..253080
/gene="yafP"
/locus_tag="b0234"
/gene_synonym="ECK0235; JW0224"
/note="Acetyltransferase (GNAT) domain; Region:
Acetyltransf_10; pfam13673"
/db_xref="CDD:222309"
misc_feature 252880..>253014
/gene="yafP"
/locus_tag="b0234"
/gene_synonym="ECK0235; JW0224"
/note="N-Acyltransferase superfamily: Various enzymes that
characteristically catalyze the transfer of an acyl group
to a substrate; Region: NAT_SF; cd04301"
/db_xref="CDD:173926"
misc_feature order(252949..252957,252985..252990)
/gene="yafP"
/locus_tag="b0234"
/gene_synonym="ECK0235; JW0224"
/note="Coenzyme A binding pocket [chemical binding]; other
site"
/db_xref="CDD:173926"
gene 253479..253685
/gene="ykfJ"
/locus_tag="b0235"
/gene_synonym="ECK0236; JW0225"
/pseudo
/db_xref="EcoGene:EG14368"
/db_xref="GeneID:944924"
CDS 253479..253685
/gene="ykfJ"
/locus_tag="b0235"
/gene_synonym="ECK0236; JW0225"
/note="pseudogene"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="ASAP:ABE-0000805"
/db_xref="UniProtKB/Swiss-Prot:P75675"
/db_xref="EcoGene:EG14368"
/db_xref="GeneID:944924"
gene 253702..254202
/gene="prfH"
/locus_tag="b0236"
/gene_synonym="ECK0237; JW0226"
/pseudo
/db_xref="EcoGene:EG11496"
/db_xref="GeneID:947374"
CDS 253702..254202
/gene="prfH"
/locus_tag="b0236"
/gene_synonym="ECK0237; JW0226"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006412 - translation"
/note="pseudogene, predicted peptide chain release factor
homolog;
probable peptide chain release factor"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="ASAP:ABE-0000807"
/db_xref="UniProtKB/Swiss-Prot:P28369"
/db_xref="EcoGene:EG11496"
/db_xref="GeneID:947374"
repeat_region 254217..254244
/note="REP20 (repetitive extragenic palindromic) element;
contains 1 REP sequences"
gene complement(254259..255716)
/gene="pepD"
/locus_tag="b0237"
/gene_synonym="ECK0238; JW0227; pepH"
/db_xref="EcoGene:EG10695"
/db_xref="GeneID:945013"
CDS complement(254259..255716)
/gene="pepD"
/locus_tag="b0237"
/gene_synonym="ECK0238; JW0227; pepH"
/EC_number="1.5.1.38"
/function="enzyme; Degradation of proteins, peptides,
glyco"
/GO_component="GO:0005737 - cytoplasm"
/codon_start=1
/transl_table=11
/product="aminoacyl-histidine dipeptidase (peptidase D)"
/protein_id="NP_414772.1"
/db_xref="GI:16128223"
/db_xref="ASAP:ABE-0000809"
/db_xref="UniProtKB/Swiss-Prot:P15288"
/db_xref="EcoGene:EG10695"
/db_xref="GeneID:945013"
/translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQGNWL
QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
mat_peptide complement(254262..255713)
/gene="pepD"
/locus_tag="b0237"
/gene_synonym="ECK0238; JW0227; pepH"
/product="aminoacyl-histidine dipeptidase (peptidase D)"
/experiment="N-terminus verified by Edman degradation:
PMID 2651887,7988883"
misc_feature complement(254271..255698)
/gene="pepD"
/locus_tag="b0237"
/gene_synonym="ECK0238; JW0227; pepH"
/note="Xaa-His dipeptidase; Region: aa-his-dipept;
TIGR01893"
/db_xref="CDD:233622"
misc_feature complement(254271..255692)
/gene="pepD"
/locus_tag="b0237"
/gene_synonym="ECK0238; JW0227; pepH"
/note="M20 Peptidase D has specificity for
beta-alanyl-L-histidine dipeptide; Region: M20_pepD;
cd03890"
/db_xref="CDD:193510"
misc_feature complement(order(254346..254348,255210..255212,
255279..255284,255372..255374,255489..255491))
/gene="pepD"
/locus_tag="b0237"
/gene_synonym="ECK0238; JW0227; pepH"
/note="metal binding site [ion binding]; metal-binding
site"
/db_xref="CDD:193510"
misc_feature complement(order(254571..254576,254583..254588,
254595..254597,254706..254711,254718..254720,
254739..254744,254748..254753,254847..254849,
254859..254861,255018..255020,255048..255050))
/gene="pepD"
/locus_tag="b0237"
/gene_synonym="ECK0238; JW0227; pepH"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:193510"
gene 255977..256435
/gene="gpt"
/locus_tag="b0238"
/gene_synonym="ECK0239; gpp; gxu; JW0228"
/db_xref="EcoGene:EG10414"
/db_xref="GeneID:944817"
CDS 255977..256435
/gene="gpt"
/locus_tag="b0238"
/gene_synonym="ECK0239; gpp; gxu; JW0228"
/EC_number="2.4.2.22"
/function="enzyme; Salvage of nucleosides and nucleotides"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0015949 - nucleobase, nucleoside and
nucleotide interconversion"
/codon_start=1
/transl_table=11
/product="guanine-hypoxanthine phosphoribosyltransferase"
/protein_id="NP_414773.1"
/db_xref="GI:16128224"
/db_xref="ASAP:ABE-0000814"
/db_xref="UniProtKB/Swiss-Prot:P0A9M5"
/db_xref="EcoGene:EG10414"
/db_xref="GeneID:944817"
/translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
misc_feature 256070..256363
/gene="gpt"
/locus_tag="b0238"
/gene_synonym="ECK0239; gpp; gxu; JW0228"
/note="Phosphoribosyl transferase (PRT)-type I domain;
Region: PRTases_typeI; cd06223"
/db_xref="CDD:206754"
misc_feature order(256082..256084,256088..256090,256238..256246,
256250..256264,256319..256321)
/gene="gpt"
/locus_tag="b0238"
/gene_synonym="ECK0239; gpp; gxu; JW0228"
/note="active site"
/db_xref="CDD:206754"
gene 256527..257771
/gene="frsA"
/locus_tag="b0239"
/gene_synonym="ECK0240; JW0229; yafA"
/db_xref="EcoGene:EG11091"
/db_xref="GeneID:946039"
CDS 256527..257771
/gene="frsA"
/locus_tag="b0239"
/gene_synonym="ECK0240; JW0229; yafA"
/codon_start=1
/transl_table=11
/product="fermentation-respiration switch protein; PTS
Enzyme IIA(Glc)-binding protein; pNP-butyrate esterase
activity"
/protein_id="NP_414774.1"
/db_xref="GI:16128225"
/db_xref="ASAP:ABE-0000817"
/db_xref="UniProtKB/Swiss-Prot:P04335"
/db_xref="EcoGene:EG11091"
/db_xref="GeneID:946039"
/translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
TQAMVWQQKACAEDDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
PVYRNFDKGLQEITDWIEKRLC"
misc_feature 256527..257768
/gene="frsA"
/locus_tag="b0239"
/gene_synonym="ECK0240; JW0229; yafA"
/note="fermentation/respiration switch protein; Reviewed;
Region: frsA; PRK05077"
/db_xref="CDD:235337"
gene 257829..258230
/gene="crl"
/locus_tag="b0240"
/gene_synonym="ECK0241; JW0230"
/db_xref="EcoGene:EG11092"
/db_xref="GeneID:945077"
CDS 257829..258230
/gene="crl"
/locus_tag="b0240"
/gene_synonym="ECK0241; JW0230"
/function="regulator; Surface structures"
/GO_component="GO:0009289 - pilus"
/GO_component="GO:0005737 - cytoplasm"
/GO_function="GO:0016563 - transcription activator
activity"
/GO_process="GO:0006350 - transcription"
/note="transcriptional regulator of cryptic csgA gene for
curli surface fibers"
/codon_start=1
/transl_table=11
/product="sigma factor-binding protein, stimulates RNA
polymerase holoenzyme formation"
/protein_id="NP_414775.1"
/db_xref="GI:16128226"
/db_xref="ASAP:ABE-0000821"
/db_xref="UniProtKB/Swiss-Prot:P24251"
/db_xref="EcoGene:EG11092"
/db_xref="GeneID:945077"
/translation="MTLPSGHPKSRLIKKFTALGPYIREGKCKDNRFFFDCLAVCVNV
KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
misc_feature 257829..258209
/gene="crl"
/locus_tag="b0240"
/gene_synonym="ECK0241; JW0230"
/note="DNA-binding transcriptional regulator Crl;
Provisional; Region: PRK10984"
/db_xref="CDD:182882"
mat_peptide 257832..258227
/gene="crl"
/locus_tag="b0240"
/gene_synonym="ECK0241; JW0230"
/product="sigma factor-binding protein, stimulates RNA
polymerase holoenzyme formation"
/experiment="N-terminus verified by Edman degradation:
PMID 2649795"
repeat_region 258240..258260
/note="REP21 (repetitive extragenic palindromic) element;
contains 1 REP sequences"
gene complement(258269..259324)
/gene="phoE"
/locus_tag="b0241"
/gene_synonym="ECK0242; JW0231; ompE"
/db_xref="EcoGene:EG10729"
/db_xref="GeneID:944926"
CDS complement(258269..259324)
/gene="phoE"
/locus_tag="b0241"
/gene_synonym="ECK0242; JW0231; ompE"
/function="membrane; Outer membrane constituents"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0009279 - cell outer membrane"
/note="outer membrane pore protein E (E,Ic,NmpAB)"
/codon_start=1
/transl_table=11
/product="outer membrane phosphoporin protein E"
/protein_id="NP_414776.1"
/db_xref="GI:16128227"
/db_xref="ASAP:ABE-0000823"
/db_xref="UniProtKB/Swiss-Prot:P02932"
/db_xref="EcoGene:EG10729"
/db_xref="GeneID:944926"
/translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
LNINNDDIVAVGMTYQF"
misc_feature complement(258272..259324)
/gene="phoE"
/locus_tag="b0241"
/gene_synonym="ECK0242; JW0231; ompE"
/note="Outer membrane protein (porin) [Cell envelope
biogenesis, outer membrane]; Region: OmpC; COG3203"
/db_xref="CDD:225744"
mat_peptide complement(258272..259261)
/gene="phoE"
/locus_tag="b0241"
/gene_synonym="ECK0242; JW0231; ompE"
/product="outer membrane phosphoporin protein E"
/experiment="N-terminus verified by Edman degradation:
PMID 337109"
misc_feature complement(258272..259237)
/gene="phoE"
/locus_tag="b0241"
/gene_synonym="ECK0242; JW0231; ompE"
/note="Porins form aqueous channels for the diffusion of
small hydrophillic molecules across the outer membrane.
Individual 16-strand anti-parallel beta-barrels form a
central pore, and trimerizes thru mainly hydrophobic
interactions at the interface. Trimers...; Region:
gram_neg_porins; cd00342"
/db_xref="CDD:238208"
misc_feature complement(order(258272..258274,258278..258280,
258284..258286,258371..258373,258380..258385,
258875..258880,258884..258886,258983..258991,
259019..259033,259037..259042,259073..259084,
259088..259090,259094..259102,259106..259108,
259124..259138,259199..259207,259211..259213,
259229..259231,259235..259237))
/gene="phoE"
/locus_tag="b0241"
/gene_synonym="ECK0242; JW0231; ompE"
/note="trimer interface [polypeptide binding]; other site"
/db_xref="CDD:238208"
misc_feature complement(order(258884..258886,258944..258946,
259037..259039,259151..259153,259214..259216))
/gene="phoE"
/locus_tag="b0241"
/gene_synonym="ECK0242; JW0231; ompE"
/note="eyelet of channel; other site"
/db_xref="CDD:238208"
gene 259612..260715
/gene="proB"
/locus_tag="b0242"
/gene_synonym="ECK0243; JW0232; pro"
/db_xref="EcoGene:EG10768"
/db_xref="GeneID:946425"
CDS 259612..260715
/gene="proB"
/locus_tag="b0242"
/gene_synonym="ECK0243; JW0232; pro"
/EC_number="2.7.2.11"
/function="enzyme; Amino acid biosynthesis: Proline"
/experiment="N-terminus verified by Edman degradation:
PMID 6089111"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006561 - proline biosynthetic process"
/codon_start=1
/transl_table=11
/product="gamma-glutamate kinase"
/protein_id="NP_414777.1"
/db_xref="GI:16128228"
/db_xref="ASAP:ABE-0000830"
/db_xref="UniProtKB/Swiss-Prot:P0A7B5"
/db_xref="EcoGene:EG10768"
/db_xref="GeneID:946425"
/translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
misc_feature 259612..260706
/gene="proB"
/locus_tag="b0242"
/gene_synonym="ECK0243; JW0232; pro"
/note="gamma-glutamyl kinase; Provisional; Region:
PRK05429"
/db_xref="CDD:235460"
misc_feature 259627..260379
/gene="proB"
/locus_tag="b0242"
/gene_synonym="ECK0243; JW0232; pro"
/note="AAK_G5K_ProB: Glutamate-5-kinase (G5K) catalyzes
glutamate-dependent ATP cleavage; G5K transfers the
terminal phosphoryl group of ATP to the gamma-carboxyl
group of glutamate, in the first and controlling step of
proline (and, in mammals, ornithine)...; Region:
AAK_G5K_ProB; cd04242"
/db_xref="CDD:239775"
misc_feature order(259651..259653,260116..260124,260131..260136,
260242..260244,260248..260250,260260..260262)
/gene="proB"
/locus_tag="b0242"
/gene_synonym="ECK0243; JW0232; pro"
/note="nucleotide binding site [chemical binding]; other
site"
/db_xref="CDD:239775"
misc_feature order(259726..259731,259738..259740,259828..259830,
259840..259842,259909..259911,259915..259917,
259957..259962,259978..259980,259987..259989,
260017..260019,260023..260028)
/gene="proB"
/locus_tag="b0242"
/gene_synonym="ECK0243; JW0232; pro"
/note="homotetrameric interface [polypeptide binding];
other site"
/db_xref="CDD:239775"
misc_feature 259762..259785
/gene="proB"
/locus_tag="b0242"
/gene_synonym="ECK0243; JW0232; pro"
/note="putative phosphate binding site [ion binding];
other site"
/db_xref="CDD:239775"
misc_feature order(259765..259767,260020..260022,260053..260055)
/gene="proB"
/locus_tag="b0242"
/gene_synonym="ECK0243; JW0232; pro"
/note="putative allosteric binding site; other site"
/db_xref="CDD:239775"
misc_feature 260437..260661
/gene="proB"
/locus_tag="b0242"
/gene_synonym="ECK0243; JW0232; pro"
/note="PUA domain; Region: PUA; pfam01472"
/db_xref="CDD:201816"
gene 260727..261980
/gene="proA"
/locus_tag="b0243"
/gene_synonym="ECK0244; JW0233; pro"
/db_xref="EcoGene:EG10767"
/db_xref="GeneID:946680"
CDS 260727..261980
/gene="proA"
/locus_tag="b0243"
/gene_synonym="ECK0244; JW0233; pro"
/EC_number="1.2.1.41"
/function="enzyme; Amino acid biosynthesis: Proline"
/experiment="N-terminus verified by Edman degradation:
PMID 6089111"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006561 - proline biosynthetic process"
/codon_start=1
/transl_table=11
/product="gamma-glutamylphosphate reductase"
/protein_id="NP_414778.1"
/db_xref="GI:16128229"
/db_xref="ASAP:ABE-0000832"
/db_xref="UniProtKB/Swiss-Prot:P07004"
/db_xref="EcoGene:EG10767"
/db_xref="GeneID:946680"
/translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
ARGPMGLEALTTYKWIGIGDYTIRA"
misc_feature 260733..261962
/gene="proA"
/locus_tag="b0243"
/gene_synonym="ECK0244; JW0233; pro"
/note="Gamma-glutamyl phosphate reductase (GPR), aldehyde
dehydrogenase families 18 and 19; Region:
ALDH_F18-19_ProA-GPR; cd07079"
/db_xref="CDD:143398"
misc_feature 260751..261944
/gene="proA"
/locus_tag="b0243"
/gene_synonym="ECK0244; JW0233; pro"
/note="gamma-glutamyl phosphate reductase; Region: proA;
TIGR00407"
/db_xref="CDD:161862"
misc_feature 261483..261485
/gene="proA"
/locus_tag="b0243"
/gene_synonym="ECK0244; JW0233; pro"
/note="putative catalytic cysteine [active]"
/db_xref="CDD:143398"
gene 262095..262170
/gene="thrW"
/locus_tag="b0244"
/gene_synonym="ECK0245; JWR0008"
/db_xref="EcoGene:EG30104"
/db_xref="GeneID:947202"
tRNA 262095..262170
/gene="thrW"
/locus_tag="b0244"
/gene_synonym="ECK0245; JWR0008"
/product="tRNA-Thr"
/note="anticodon: CGU"
/db_xref="ASAP:ABE-0000834"
/db_xref="EcoGene:EG30104"
/db_xref="GeneID:947202"
misc_feature 262182..296489
/note="cryptic prophage CP4-6"
gene complement(262374..262436)
/gene="ykfN"
/locus_tag="b4626"
/gene_synonym="ECK4458"
/pseudo
/db_xref="EcoGene:EG14454"
/db_xref="GeneID:5625552"
CDS complement(262374..262436)
/gene="ykfN"
/locus_tag="b4626"
/gene_synonym="ECK4458"
/note="pseudogene, YdiA family"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="EcoGene:EG14454"
/db_xref="GeneID:5625552"
gene complement(262552..262893)
/gene="ykfI"
/locus_tag="b0245"
/gene_synonym="ECK0246; JW0234"
/db_xref="EcoGene:EG14333"
/db_xref="GeneID:946726"
CDS complement(262552..262893)
/gene="ykfI"
/locus_tag="b0245"
/gene_synonym="ECK0246; JW0234"
/function="Phage or Prophage Related"
/codon_start=1
/transl_table=11
/product="CP4-6 prophage; toxin of the YkfI-YafW
toxin-antitoxin system"
/protein_id="NP_414779.1"
/db_xref="GI:16128230"
/db_xref="ASAP:ABE-0000836"
/db_xref="UniProtKB/Swiss-Prot:P77692"
/db_xref="EcoGene:EG14333"
/db_xref="GeneID:946726"
/translation="MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFC
NEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGFSWQEQSPYLRAADILRARQATG
LLRQSRNNVVR"
misc_feature complement(262579..262893)
/gene="ykfI"
/locus_tag="b0245"
/gene_synonym="ECK0246; JW0234"
/note="Protein of unknown function (DUF1219); Region:
DUF1219; pfam06755"
/db_xref="CDD:148388"
gene complement(262914..263231)
/gene="yafW"
/locus_tag="b0246"
/gene_synonym="ECK0247; JW0235"
/db_xref="EcoGene:EG13335"
/db_xref="GeneID:944929"
CDS complement(262914..263231)
/gene="yafW"
/locus_tag="b0246"
/gene_synonym="ECK0247; JW0235"
/function="Phage or Prophage Related"
/codon_start=1
/transl_table=11
/product="CP4-6 prophage; antitoxin of the YkfI-YafW
toxin-antitoxin system"
/protein_id="NP_414780.1"
/db_xref="GI:16128231"
/db_xref="ASAP:ABE-0000838"
/db_xref="UniProtKB/Swiss-Prot:Q47684"
/db_xref="EcoGene:EG13335"
/db_xref="GeneID:944929"
/translation="MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIE
CRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYHNDLTCEADTLGSCGYVYIAIYP
TQR"
misc_feature complement(262917..263228)
/gene="yafW"
/locus_tag="b0246"
/gene_synonym="ECK0247; JW0235"
/note="YagB/YeeU/YfjZ family; Region: YagB_YeeU_YfjZ;
pfam06154"
/db_xref="CDD:114849"
gene complement(263250..263471)
/gene="ykfH"
/locus_tag="b4504"
/gene_synonym="ECK0248; JW5956"
/db_xref="EcoGene:EG14332"
/db_xref="GeneID:1450236"
CDS complement(263250..263471)
/gene="ykfH"
/locus_tag="b4504"
/gene_synonym="ECK0248; JW5956"
/GO_component="GO:0005737 - cytoplasm"
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="YP_588435.1"
/db_xref="GI:94541094"
/db_xref="ASAP:ABE-0285025"
/db_xref="UniProtKB/Swiss-Prot:Q9XB42"
/db_xref="EcoGene:EG14332"
/db_xref="GeneID:1450236"
/translation="MKIISKRRAMTIYRQHPESRIFRYCTGKYQWHGSVCHYTGRDVP
DIAGVLAVYAERRQDRNGPYTCLMSITLN"
misc_feature complement(263274..263471)
/gene="ykfH"
/locus_tag="b4504"
/gene_synonym="ECK0248; JW5956"
/note="Protein of unknown function (DUF987); Region:
DUF987; pfam06174"
/db_xref="CDD:114867"
gene complement(263480..263956)
/gene="ykfG"
/locus_tag="b0247"
/gene_synonym="ECK0249; JW0236"
/db_xref="EcoGene:EG14331"
/db_xref="GeneID:944930"
CDS complement(263480..263956)
/gene="ykfG"
/locus_tag="b0247"
/gene_synonym="ECK0249; JW0236"
/function="Phage or Prophage Related"
/note="putative DNA repair protein"
/codon_start=1
/transl_table=11
/product="CP4-6 prophage; predicted DNA repair protein"
/protein_id="NP_414781.1"
/db_xref="GI:16128232"
/db_xref="ASAP:ABE-0000841"
/db_xref="UniProtKB/Swiss-Prot:Q47685"
/db_xref="EcoGene:EG14331"
/db_xref="GeneID:944930"
/translation="MKQLSFLPGEMTPQDRRLIQRALRALDRHLHEPGVAFTSTHAVR
EWLRLHMAALEREEFRVLYLDNQNQLIAHETLFTGTINRTEVHPREVVKRALHFNAAA
VILAHNHPSGETTPSQADKTLTQRLVQVLQLVDIRVPDHLIVGGRQIYSFAEHGLL"
misc_feature complement(263483..>263956)
/gene="ykfG"
/locus_tag="b0247"
/gene_synonym="ECK0249; JW0236"
/note="DNA repair proteins [DNA replication,
recombination, and repair]; Region: RadC; COG2003"
/db_xref="CDD:224914"
misc_feature complement(263498..263836)
/gene="ykfG"
/locus_tag="b0247"
/gene_synonym="ECK0249; JW0236"
/note="Mov34/MPN/PAD-1 family; Region: MPN_DUF2466;
cd08071"
/db_xref="CDD:163702"
misc_feature complement(order(263597..263599,263606..263608,
263630..263632,263636..263638,263786..263788))
/gene="ykfG"
/locus_tag="b0247"
/gene_synonym="ECK0249; JW0236"
/note="MPN+ (JAMM) motif; other site"
/db_xref="CDD:163702"
misc_feature complement(order(263597..263599,263630..263632,
263636..263638))
/gene="ykfG"
/locus_tag="b0247"
/gene_synonym="ECK0249; JW0236"
/note="Zinc-binding site [ion binding]; other site"
/db_xref="CDD:163702"
gene complement(263972..264430)
/gene="yafX"
/locus_tag="b0248"
/gene_synonym="ECK0250; JW5022"
/db_xref="EcoGene:EG13336"
/db_xref="GeneID:944931"
CDS complement(263972..264430)
/gene="yafX"
/locus_tag="b0248"
/gene_synonym="ECK0250; JW5022"
/function="Phage or Prophage Related"
/codon_start=1
/transl_table=11
/product="CP4-6 prophage; putative protein"
/protein_id="NP_414782.1"
/db_xref="GI:16128233"
/db_xref="ASAP:ABE-0000843"
/db_xref="UniProtKB/Swiss-Prot:P75676"
/db_xref="EcoGene:EG13336"
/db_xref="GeneID:944931"
/translation="MTTQTQHDLAPANQPEFELTVTPVPDEQRIDFWPQYFGAIPQWL
LLEPHIFAWMDRFCEGYSGGIWSFYTLSNGGAFMSPEPDNDETWRLFNCLNGNDAQMS
AEAAGIAVCLIAYSHHACRTECDAMTAHYYRLREYAMQHPEAHAILRIID"
misc_feature complement(263975..264262)
/gene="yafX"
/locus_tag="b0248"
/gene_synonym="ECK0250; JW5022"
/note="Antirestriction protein; Region: Antirestrict;
pfam03230"
/db_xref="CDD:190572"
gene complement(264528..264767)
/gene="ykfF"
/locus_tag="b0249"
/gene_synonym="ECK0251; JW5023"
/db_xref="EcoGene:EG14283"
/db_xref="GeneID:948839"
CDS complement(264528..264767)
/gene="ykfF"
/locus_tag="b0249"
/gene_synonym="ECK0251; JW5023"
/function="Phage or Prophage Related"
/codon_start=1
/transl_table=11
/product="CP4-6 prophage; putative protein"
/protein_id="NP_414783.1"
/db_xref="GI:16128234"
/db_xref="ASAP:ABE-0000846"
/db_xref="UniProtKB/Swiss-Prot:P75677"
/db_xref="EcoGene:EG14283"
/db_xref="GeneID:948839"
/translation="MTQSVLLPPGPFTRRQAQAVTTTYSNITLEDDQGSHFRLVVRDT
EGRMVWRAWNFEPDAGEGLNRYIRTSGIRTDTATR"
misc_feature complement(264546..264758)
/gene="ykfF"
/locus_tag="b0249"
/gene_synonym="ECK0251; JW5023"
/note="Bacterial protein of unknown function (DUF905);
Region: DUF905; pfam06006"
/db_xref="CDD:114712"
gene complement(264844..265311)
/gene="ykfB"
/locus_tag="b0250"
/gene_synonym="ECK0252; JW0239"
/db_xref="EcoGene:EG13548"
/db_xref="GeneID:946846"
CDS complement(264844..265311)
/gene="ykfB"
/locus_tag="b0250"
/gene_synonym="ECK0252; JW0239"
/function="Phage or Prophage Related"
/codon_start=1
/transl_table=11
/product="CP4-6 prophage; putative protein"
/protein_id="NP_414784.1"
/db_xref="GI:16128235"
/db_xref="ASAP:ABE-0000851"
/db_xref="UniProtKB/Swiss-Prot:P77162"
/db_xref="EcoGene:EG13548"
/db_xref="GeneID:946846"
/translation="MTILSLSRFMLAGVLLASFNASAIPGFWQQGYGQGNTEYSVTEA
SGKTFTINCTGNPDQNGFYQHSVFLTLADDKMVSSHDDDTTITVVMDHQQYIIPSSLG
WRNGDNAWFDFISNISEAGQFDVYVNDHKAGTFTADRKNAEKVLSTLGDCSND"
gene complement(265334..265777)
/gene="yafY"
/locus_tag="b0251"
/gene_synonym="ECK0253; JW0240"
/db_xref="EcoGene:EG13337"
/db_xref="GeneID:944934"
CDS complement(265334..265777)
/gene="yafY"
/locus_tag="b0251"
/gene_synonym="ECK0253; JW0240"
/function="Phage or Prophage Related"
/note="hypothetical transcriptional regulator"
/codon_start=1
/transl_table=11
/product="lipoprotein, inner membrane; overproduction
stimulates degP expression; CP4-6 prophage"
/protein_id="NP_414785.4"
/db_xref="GI:145698221"
/db_xref="ASAP:ABE-0000853"
/db_xref="UniProtKB/Swiss-Prot:P77365"
/db_xref="EcoGene:EG13337"
/db_xref="GeneID:944934"
/translation="MKRKTLPLLALVATTLFLIACDDRSDDLKAISKFKDLTPPRFSD
VVSHQDDVSEEWSQVDYLSGPTLQVLRTRQSPDGCEDGSYYYLVDMQEKTVQPLMNAL
CIADNIKLEYQEVTDPYTKEKYFEYAHDGKLMGQLLIPSNPDNQE"
misc_feature complement(265340..>265702)
/gene="yafY"
/locus_tag="b0251"
/gene_synonym="ECK0253; JW0240"
/note="Predicted transcriptional regulator
[Transcription]; Region: COG2378"
/db_xref="CDD:225253"
gene complement(265777..265998)
/gene="ykfL"
/locus_tag="b4627"
/gene_synonym="ECK4445"
/pseudo
/db_xref="EcoGene:EG14378"
/db_xref="GeneID:5625553"
CDS complement(265777..265998)
/gene="ykfL"
/locus_tag="b4627"
/gene_synonym="ECK4445"
/note="pseudogene, CP4-6 putative prophage remnant;Phage
or Prophage Related"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="EcoGene:EG14378"
/db_xref="GeneID:5625553"
gene complement(266000..266191)
/gene="ykfK"
/locus_tag="b4628"
/gene_synonym="ECK4444; yafY"
/pseudo
/db_xref="EcoGene:EG14377"
/db_xref="GeneID:5625554"
CDS complement(266000..266191)
/gene="ykfK"
/locus_tag="b4628"
/gene_synonym="ECK4444; yafY"
/note="CP4-6 prophage; predicted DNA-binding
transcriptional regulator;Phage or Prophage Related"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="EcoGene:EG14377"
/db_xref="GeneID:5625554"
gene complement(266408..267229)
/gene="yafZ"
/locus_tag="b0252"
/gene_synonym="ECK0254; JW0242"
/db_xref="EcoGene:EG13338"
/db_xref="GeneID:945373"
CDS complement(266408..267229)
/gene="yafZ"
/locus_tag="b0252"
/gene_synonym="ECK0254; JW0242"
/function="Phage or Prophage Related"
/codon_start=1
/transl_table=11
/product="CP4-6 prophage; conserved protein"
/protein_id="NP_414786.4"
/db_xref="GI:90111102"
/db_xref="ASAP:ABE-0000856"
/db_xref="UniProtKB/Swiss-Prot:P77206"
/db_xref="EcoGene:EG13338"
/db_xref="GeneID:945373"
/translation="MTRLASRFGAANLIRRDRPLTREELFRVVPSVFSEDKHESRSER
YTYIPTISLLDSLQREGFQPFFACQTRVRDPGRREHTKHMLRLRREGQITGKQVPEII
LLNSHDGTSSYQMLPGLFRAVCQNGLVCGESFGEVRVPHKGDVVSQVIEGAYEVLGIF
DRVEEKRDAMQSLLLPPPAQQALAKAALTYRFGEDHQPVTESQILSPRRWQDESNDLW
TTYQRIQENLIKGGLSGRNAKGGRSHTRAVRGIDGDVKLNRALWVMAEALLTQLQ"
misc_feature complement(266444..267121)
/gene="yafZ"
/locus_tag="b0252"
/gene_synonym="ECK0254; JW0242"
/note="Domain of unknown function (DUF932); Region:
DUF932; pfam06067"
/db_xref="CDD:147948"
gene complement(267321..268184)
/gene="ykfA"
/locus_tag="b0253"
/gene_synonym="ECK0255; JW0243"
/db_xref="EcoGene:EG13339"
/db_xref="GeneID:949004"
CDS complement(267321..268184)
/gene="ykfA"
/locus_tag="b0253"
/gene_synonym="ECK0255; JW0243"
/function="Phage or Prophage Related"
/note="putative GTP-binding protein"
/codon_start=1
/transl_table=11
/product="CP4-6 prophage; predicted GTP-binding protein"
/protein_id="NP_414787.4"
/db_xref="GI:90111103"
/db_xref="ASAP:ABE-0000858"
/db_xref="UniProtKB/Swiss-Prot:P75678"
/db_xref="EcoGene:EG13339"
/db_xref="GeneID:949004"
/translation="MNNSEGLKSFQQSLADLPQWVSERLLQQINQLTNYEPVIGIMGK
TGVGKSSLCNALFAGDISPVSDVAACTREPLRFRLQVGDRYITLMDLPGVGESGARDT
EYAALYREQLPRLDLVLWLIKADDRALTVDEHFYHQVIGEVYRHKVLFVISQSDKAEP
TSGGGQLSTAQKQNISRKICLLHELFQPVHPVCAVSVRLQWGLKVMAERMIKCLPREA
TSPVVSQLHPSFRTTVVREQARSDFGETVGAVLDSISAFPLIPAPVRAVIQAVRTTVV
SVARAVWDFFF"
misc_feature complement(267324..268184)
/gene="ykfA"
/locus_tag="b0253"
/gene_synonym="ECK0255; JW0243"
/note="Predicted GTPase [General function prediction
only]; Region: COG3596"
/db_xref="CDD:33396"
misc_feature complement(267543..268067)
/gene="ykfA"
/locus_tag="b0253"
/gene_synonym="ECK0255; JW0243"
/note="YfjP GTPase; Region: YfjP; cd11383"
/db_xref="CDD:206743"
misc_feature complement(268035..268058)
/gene="ykfA"
/locus_tag="b0253"
/gene_synonym="ECK0255; JW0243"
/note="G1 box; other site"
/db_xref="CDD:206743"
misc_feature complement(order(267594..267599,267717..267719,
267723..267728,268032..268049))
/gene="ykfA"
/locus_tag="b0253"
/gene_synonym="ECK0255; JW0243"
/note="GTP/Mg2+ binding site [chemical binding]; other
site"
/db_xref="CDD:206743"
misc_feature complement(267963..267977)
/gene="ykfA"
/locus_tag="b0253"
/gene_synonym="ECK0255; JW0243"
/note="Switch I region; other site"
/db_xref="CDD:206743"
misc_feature complement(267972..267974)
/gene="ykfA"
/locus_tag="b0253"
/gene_synonym="ECK0255; JW0243"
/note="G2 box; other site"
/db_xref="CDD:206743"
misc_feature complement(order(267843..267845,267903..267920))
/gene="ykfA"
/locus_tag="b0253"
/gene_synonym="ECK0255; JW0243"
/note="Switch II region; other site"
/db_xref="CDD:206743"
misc_feature complement(267906..267917)
/gene="ykfA"
/locus_tag="b0253"
/gene_synonym="ECK0255; JW0243"
/note="G3 box; other site"
/db_xref="CDD:206743"
misc_feature complement(267717..267728)
/gene="ykfA"
/locus_tag="b0253"
/gene_synonym="ECK0255; JW0243"
/note="G4 box; other site"
/db_xref="CDD:206743"
misc_feature complement(267591..267599)
/gene="ykfA"
/locus_tag="b0253"
/gene_synonym="ECK0255; JW0243"
/note="G5 box; other site"
/db_xref="CDD:206743"
gene complement(268513..269406)
/gene="perR"
/locus_tag="b0254"
/gene_synonym="ECK0256; JW0244"
/db_xref="EcoGene:EG13340"
/db_xref="GeneID:945417"
CDS complement(268513..269406)
/gene="perR"
/locus_tag="b0254"
/gene_synonym="ECK0256; JW0244"
/function="Phage or Prophage Related"
/note="peroxide resistance protein"
/codon_start=1
/transl_table=11
/product="CP4-6 prophage; predicted DNA-binding
transcriptional regulator"
/protein_id="NP_414788.1"
/db_xref="GI:16128239"
/db_xref="ASAP:ABE-0000862"
/db_xref="UniProtKB/Swiss-Prot:Q57083"
/db_xref="EcoGene:EG13340"
/db_xref="GeneID:945417"
/translation="MKLLAKAPLNLLRAFEAAGRTGAFALAASELELSPSAISHAIRK
LENLLDVRLFQRSTREITLTKEGEILLEHIQRGFNELQQGLALVTADESRPLRLHTAP
SFAHQWLLPRLGKFIRENPSIDLRLSASTEYARFEQDDFDLDIVYGEPRPSPYEKIPL
AVEELTPLCSPQLAERLKKPEDLYALTLIQCDVQLYQWKGWFEANKMTPPNNYGLRFD
RSFMAIAAAVDGLGVVLESKLLAEREIASGKLVCPLVNSTSEIHYIGHYLVFPQHQHM
HSALDVFKTWLLNELNLGKIR"
misc_feature complement(268531..269385)
/gene="perR"
/locus_tag="b0254"
/gene_synonym="ECK0256; JW0244"
/note="DNA-binding transcriptional activator GcvA;
Provisional; Region: PRK11139"
/db_xref="CDD:182990"
misc_feature complement(269203..269382)
/gene="perR"
/locus_tag="b0254"
/gene_synonym="ECK0256; JW0244"
/note="Bacterial regulatory helix-turn-helix protein, lysR
family; Region: HTH_1; pfam00126"
/db_xref="CDD:201021"
misc_feature complement(268546..269124)
/gene="perR"
/locus_tag="b0254"
/gene_synonym="ECK0256; JW0244"
/note="The C-terminal substrate domain of LysR-type GcdR,
TrPI, HvR and beta-lactamase regulators, and that of other
closely related homologs; contains the type 2 periplasmic
binding fold; Region: PBP2_GcdR_TrpI_HvrB_AmpR_like;
cd08432"
/db_xref="CDD:176123"
misc_feature complement(order(269020..269040,269044..269097,
269101..269124))
/gene="perR"
/locus_tag="b0254"
/gene_synonym="ECK0256; JW0244"
/note="dimerization interface [polypeptide binding]; other
site"
/db_xref="CDD:176123"
misc_feature complement(order(268687..268689,268747..268755,
269095..269097,269101..269106))
/gene="perR"
/locus_tag="b0254"
/gene_synonym="ECK0256; JW0244"
/note="substrate binding pocket [chemical binding]; other
site"
/db_xref="CDD:176123"
mobile_element 269430..269764
/mobile_element_type="insertion sequence:IS911A"
gene join(269502..269759,270988..271413)
/gene="insN"
/locus_tag="b4587"
/gene_synonym="ECK0257; insN1; insO; ORFAB"
/pseudo
/db_xref="EcoGene:EG40014"
/db_xref="GeneID:5061501"
CDS join(269502..269759,270988..271413)
/gene="insN"
/locus_tag="b4587"
/gene_synonym="ECK0257; insN1; insO; ORFAB"
/experiment="N-terminus verified by Edman degradation:
PMID 1660923"
/note="pseudogene, IS911 transposase A;IS, phage, Tn;
Transposon-related functions; extrachromosomal; transposon
related"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="ASAP:ABE-0000867"
/db_xref="UniProtKB/Swiss-Prot:P75679"
/db_xref="EcoGene:EG40014"
/db_xref="GeneID:5061501"
mobile_element 269765..270985
/mobile_element_type="insertion sequence:IS30A"
gene 269827..270978
/gene="insI1"
/locus_tag="b0256"
/gene_synonym="ECK0258"
/db_xref="EcoGene:EG40009"
/db_xref="GeneID:949005"
CDS 269827..270978
/gene="insI1"
/locus_tag="b0256"
/gene_synonym="ECK0258"
/function="IS, phage, Tn; Transposon-related functions;
extrachromosomal; transposon related"
/codon_start=1
/transl_table=11
/product="IS30 transposase"
/protein_id="NP_414790.1"
/db_xref="GI:16128241"
/db_xref="ASAP:ABE-0000871"
/db_xref="UniProtKB/Swiss-Prot:P0CF88"
/db_xref="EcoGene:EG40009"
/db_xref="GeneID:949005"
/translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIIVRLRGKDSVSVNQALT
DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIR
QYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
misc_feature 269980..270975
/gene="insI1"
/locus_tag="b0256"
/gene_synonym="ECK0258"
/note="Transposase and inactivated derivatives, IS30
family [DNA replication, recombination, and repair];
Region: Tra8; COG2826"
/db_xref="CDD:32654"
misc_feature order(269980..269988,270091..270096,270100..270105)
/gene="insI1"
/locus_tag="b0256"
/gene_synonym="ECK0258"
/note="DNA-binding interface [nucleotide binding]; DNA
binding site"
/db_xref="CDD:119388"
misc_feature 269998..270120
/gene="insI1"
/locus_tag="b0256"
/gene_synonym="ECK0258"
/note="Helix-turn-helix domain; Region: HTH_38; pfam13936"
/db_xref="CDD:206107"
misc_feature 270094..270345
/gene="insI1"
/locus_tag="b0256"
/gene_synonym="ECK0258"
/note="Homeodomain-like domain; Region: HTH_32; pfam13565"
/db_xref="CDD:205743"
misc_feature 270517..270837
/gene="insI1"
/locus_tag="b0256"
/gene_synonym="ECK0258"
/note="Integrase core domain; Region: rve; pfam00665"
/db_xref="CDD:201381"
mobile_element 270986..271414
/mobile_element_type="insertion sequence:IS911A"
gene 271804..271878
/gene="eyeA"
/locus_tag="b4690"
/gene_synonym="ECK4506; I001"
/db_xref="EcoGene:EG31183"
/db_xref="GeneID:7751631"
ncRNA 271804..271878
/gene="eyeA"
/locus_tag="b4690"
/gene_synonym="ECK4506; I001"
/ncRNA_class="other"
/product="novel sRNA, function unknown, CP4-6 propahge"
/db_xref="EcoGene:EG31183"
/db_xref="GeneID:7751631"
gene 272071..273178
/gene="ykfC"
/locus_tag="b0258"
/gene_synonym="ECK0260; JW5813"
/pseudo
/db_xref="EcoGene:EG13341"
/db_xref="GeneID:944940"
CDS 272071..273178
/gene="ykfC"
/locus_tag="b0258"
/gene_synonym="ECK0260; JW5813"
/note="CP4-6 prophage; conserved protein;Phage or Prophage
Related"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="ASAP:ABE-0000885"
/db_xref="UniProtKB/Swiss-Prot:Q47688"
/db_xref="EcoGene:EG13341"
/db_xref="GeneID:944940"
mobile_element complement(273179..274373)
/mobile_element_type="insertion sequence:IS5A"
gene complement(273325..274341)
/gene="insH1"
/locus_tag="b0259"
/gene_synonym="ECK0261"
/db_xref="EcoGene:EG40008"
/db_xref="GeneID:944941"
CDS complement(273325..274341)
/gene="insH1"
/locus_tag="b0259"
/gene_synonym="ECK0261"
/function="IS, phage, Tn; Transposon-related functions;
extrachromosomal; transposon related"
/codon_start=1
/transl_table=11
/product="IS5 transposase and trans-activator"
/protein_id="NP_414793.1"
/db_xref="GI:16128244"
/db_xref="ASAP:ABE-0000888"
/db_xref="UniProtKB/Swiss-Prot:P0CE49"
/db_xref="EcoGene:EG40008"
/db_xref="GeneID:944941"
/translation="MFVIWSHRTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQIL
PWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLF
ARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIE
APSSTKNKEQQRDPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQL
GNLLHGEEQFVSADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINI
EYMKASIRARVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQW
ERSH"
misc_feature complement(273928..274155)
/gene="insH1"
/locus_tag="b0259"
/gene_synonym="ECK0261"
/note="Transposase domain (DUF772); Region: DUF772;
pfam05598"
/db_xref="CDD:203284"
misc_feature complement(273340..274119)
/gene="insH1"
/locus_tag="b0259"
/gene_synonym="ECK0261"
/note="Transposase and inactivated derivatives, IS5 family
[DNA replication, recombination, and repair]; Region:
COG3039"
/db_xref="CDD:32853"
misc_feature complement(273370..>273531)
/gene="insH1"
/locus_tag="b0259"
/gene_synonym="ECK0261"
/note="DDE superfamily endonuclease; Region: DDE_4;
cl15789"
/db_xref="CDD:211471"
gene 274549..275952
/gene="mmuP"
/locus_tag="b0260"
/gene_synonym="ECK0262; JW5027; ykfD"
/db_xref="EcoGene:EG13342"
/db_xref="GeneID:946284"
CDS 274549..275952
/gene="mmuP"
/locus_tag="b0260"
/gene_synonym="ECK0262; JW5027; ykfD"
/function="Phage or Prophage Related"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/GO_process="GO:0009086 - methionine biosynthetic process"
/codon_start=1
/transl_table=11
/product="CP4-6 prophage; predicted S-methylmethionine
transporter"
/protein_id="NP_414794.4"
/db_xref="GI:90111105"
/db_xref="ASAP:ABE-0000893"
/db_xref="UniProtKB/Swiss-Prot:Q47689"
/db_xref="EcoGene:EG13342"
/db_xref="GeneID:946284"
/translation="MQTTQQNAPLKRTMKTRHLIMLSLGGVIGTGLFFNTGYIISTTG
AAGTLLAYLIGALVVWLVMQCLGELSVAMPETGAFHVYAARYLGPATGYTVAWLYWLT
WTVALGSSFTAAGFCMQYWFPQVPVWVWCVVFCAIIFGLNVISTRFFAEGEFWFSLVK
VVTIIAFIILGGAAIFGFIPMQDGSPAPGLSNITAEGWFPHGGLPILMTMVAVNFAFS
GTELIGIAAGETENPRKVIPVAIRTTIARLIIFFIGTVFVLAALIPMQQVGVEKSPFV
LVFEKVGIPYAADIFNFVILTAILSAANSGLYASGRMLWSLSNERTLPACFARVTKNG
VPLTALSVSMLGGVLALFSSVVAPDTVFVALSAISGFAVVAVWLSICASHFVFRRRHL
QQGKALSELHYRAPWYPLVPVLGFVLCLVACVGLAFDPAQRIALWCGLPFVALCYGAY
FLTQPRNAKQEPEHVAE"
misc_feature 274549..275949
/gene="mmuP"
/locus_tag="b0260"
/gene_synonym="ECK0262; JW5027; ykfD"
/note="S-methylmethionine transporter; Provisional;
Region: PRK11387"
/db_xref="CDD:183113"
gene 275939..276871
/gene="mmuM"
/locus_tag="b0261"
/gene_synonym="ECK0263; JW0253; yagD"
/db_xref="EcoGene:EG13343"
/db_xref="GeneID:946143"
CDS 275939..276871
/gene="mmuM"
/locus_tag="b0261"
/gene_synonym="ECK0263; JW0253; yagD"
/EC_number="2.1.1.10"
/function="Phage or Prophage Related"
/GO_process="GO:0006071 - glycerol metabolic process"
/GO_process="GO:0009086 - methionine biosynthetic process"
/codon_start=1
/transl_table=11
/product="CP4-6 prophage; S-methylmethionine:homocysteine
methyltransferase"
/protein_id="NP_414795.1"
/db_xref="GI:16128246"
/db_xref="ASAP:ABE-0000895"
/db_xref="UniProtKB/Swiss-Prot:Q47690"
/db_xref="EcoGene:EG13343"
/db_xref="GeneID:946143"
/translation="MSQNNPLRALLDKQDILLLDGAMATELEARGCNLADSLWSAKVL
VENPELIREVHLDYYRAGAQCAITASYQATPAGFAARGLDEAQSKALIGKSVELARKA
REAYLAENPQAGTLLVAGSVGPYGAYLADGSEYRGDYHCSVEAFQAFHRPRVEALLDA
GADLLACETLPNFSEIEALAELLTAYPRARAWFSFTLRDSEHLSDGTPLRDVVALLAG
YPQVVALGINCIALENTTAALQHLHGLTVLPLVVYPNSGEHYDAVSKTWHHHGEHCAQ
LADYLPQWQAAGARLIGGCCRTTPADIAALKARS"
misc_feature 275948..276865
/gene="mmuM"
/locus_tag="b0261"
/gene_synonym="ECK0263; JW0253; yagD"
/note="homocysteine methyltransferase; Provisional;
Region: mmuM; PRK09485"
/db_xref="CDD:181899"
misc_feature 275984..276862
/gene="mmuM"
/locus_tag="b0261"
/gene_synonym="ECK0263; JW0253; yagD"
/note="Homocysteine S-methyltransferase; Region:
S-methyl_trans; pfam02574"
/db_xref="CDD:217114"
repeat_region 276899..276965
/note="REP22 (repetitive extragenic palindromic) element;
contains 2 REP sequences"
gene complement(276980..278026)
/gene="afuC"
/locus_tag="b0262"
/gene_synonym="ECK0264; fbpC; JW0254; yagC"
/db_xref="EcoGene:EG12340"
/db_xref="GeneID:947676"
CDS complement(276980..278026)
/gene="afuC"
/locus_tag="b0262"
/gene_synonym="ECK0264; fbpC; JW0254; yagC"
/function="putative transport; Not classified; Phage or
Prophage Related"
/note="putative ATP-binding component of a transport
system"
/codon_start=1
/transl_table=11
/product="CP4-6 prophage; predicted ferric transporter
subunit"
/protein_id="NP_414796.2"
/db_xref="GI:90111106"
/db_xref="ASAP:ABE-0000898"
/db_xref="UniProtKB/Swiss-Prot:P37009"
/db_xref="EcoGene:EG12340"
/db_xref="GeneID:947676"
/translation="MTQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
LKMLGVPRAELKARVKEALAMVDLEGFEDRFVDQISGGQQQRVALARALILKPKVLLF
DEPLSNLDANLRRSMRDKIRELQKQFDITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
GSPQDLYRQPASRFMASFMGDANLFPATFSDGYVDIYGYHLPRPLHFGTQGEGMVGVR
PEAITLSDRGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDVGEQYYL
EIHPYGMFVLADAA"
misc_feature complement(276983..278026)
/gene="afuC"
/locus_tag="b0262"
/gene_synonym="ECK0264; fbpC; JW0254; yagC"
/note="ferric transporter ATP-binding subunit;
Provisional; Region: fbpC; PRK11432"
/db_xref="CDD:183133"
misc_feature complement(277370..278008)
/gene="afuC"
/locus_tag="b0262"
/gene_synonym="ECK0264; fbpC; JW0254; yagC"
/note="ATP-binding cassette domain of the carbohydrate and
solute transporters-like; Region: ABC_Carb_Solutes_like;
cd03259"
/db_xref="CDD:213226"
misc_feature complement(277889..277912)
/gene="afuC"
/locus_tag="b0262"
/gene_synonym="ECK0264; fbpC; JW0254; yagC"
/note="Walker A/P-loop; other site"
/db_xref="CDD:213226"
misc_feature complement(order(277442..277444,277541..277546,
277772..277774,277886..277894,277898..277903))
/gene="afuC"
/locus_tag="b0262"
/gene_synonym="ECK0264; fbpC; JW0254; yagC"
/note="ATP binding site [chemical binding]; other site"
/db_xref="CDD:213226"
misc_feature complement(277772..277783)
/gene="afuC"
/locus_tag="b0262"
/gene_synonym="ECK0264; fbpC; JW0254; yagC"
/note="Q-loop/lid; other site"
/db_xref="CDD:213226"
misc_feature complement(277589..277618)
/gene="afuC"
/locus_tag="b0262"
/gene_synonym="ECK0264; fbpC; JW0254; yagC"
/note="ABC transporter signature motif; other site"
/db_xref="CDD:213226"
misc_feature complement(277541..277558)
/gene="afuC"
/locus_tag="b0262"
/gene_synonym="ECK0264; fbpC; JW0254; yagC"
/note="Walker B; other site"
/db_xref="CDD:213226"
misc_feature complement(277523..277534)
/gene="afuC"
/locus_tag="b0262"
/gene_synonym="ECK0264; fbpC; JW0254; yagC"
/note="D-loop; other site"
/db_xref="CDD:213226"
misc_feature complement(277436..277456)
/gene="afuC"
/locus_tag="b0262"
/gene_synonym="ECK0264; fbpC; JW0254; yagC"
/note="H-loop/switch region; other site"
/db_xref="CDD:213226"
misc_feature complement(276995..277210)
/gene="afuC"
/locus_tag="b0262"
/gene_synonym="ECK0264; fbpC; JW0254; yagC"
/note="TOBE domain; Region: TOBE_2; pfam08402"
/db_xref="CDD:219823"
gene complement(278038..278385)
/gene="afuB"
/locus_tag="b0263"
/gene_synonym="ECK0265; fbpB; JW0255"
/pseudo
/db_xref="EcoGene:EG14277"
/db_xref="GeneID:947073"
CDS complement(278038..278385)
/gene="afuB"
/locus_tag="b0263"
/gene_synonym="ECK0265; fbpB; JW0255"
/note="pseudogene, CP4-6 prophage; predicted ferric
transporter subunit;Phage or Prophage Related"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="ASAP:ABE-0000900"
/db_xref="UniProtKB/Swiss-Prot:P75681"
/db_xref="EcoGene:EG14277"
/db_xref="GeneID:947073"
mobile_element complement(278387..279154)
/mobile_element_type="insertion sequence:IS1B"
gene complement(278402..278905)
/gene="insB1"
/locus_tag="b0264"
/gene_synonym="ECK0022"
/db_xref="EcoGene:EG40002"
/db_xref="GeneID:947698"
CDS complement(278402..278905)
/gene="insB1"
/locus_tag="b0264"
/gene_synonym="ECK0022"
/function="IS, phage, Tn; Transposon-related functions;
extrachromosomal; transposon related"
/codon_start=1
/transl_table=11
/product="IS1 transposase B"
/protein_id="NP_414798.1"
/db_xref="GI:16128249"
/db_xref="ASAP:ABE-0000906"
/db_xref="UniProtKB/Swiss-Prot:P0CF26"
/db_xref="EcoGene:EG40002"
/db_xref="GeneID:947698"
/translation="MPGNRPHYGRWPQHDFPPFKKLRPQSVTSRIQPGSDVIVCAEMD
EQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDG
WPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVEQHDKVIGHY
LNIKHYQ"
misc_feature complement(278405..278797)
/gene="insB1"
/locus_tag="b0264"
/gene_synonym="ECK0022"
/note="IS1 transposase; Region: DDE_Tnp_IS1; pfam03400"
/db_xref="CDD:112225"
gene complement(278824..279099)
/gene="insA"
/locus_tag="b0265"
/gene_synonym="ECK4521"
/note="insA2"
/db_xref="EcoGene:EG40001"
/db_xref="GeneID:944946"
CDS complement(278824..279099)
/gene="insA"
/locus_tag="b0265"
/gene_synonym="ECK4521"
/function="IS, phage, Tn; Transposon-related functions;
extrachromosomal; transposon related"
/codon_start=1
/transl_table=11
/product="IS1 repressor TnpA"
/protein_id="NP_414799.1"
/db_xref="GI:16128250"
/db_xref="ASAP:ABE-0000909"
/db_xref="UniProtKB/Swiss-Prot:P0CF08"
/db_xref="EcoGene:EG40001"
/db_xref="GeneID:944946"
/translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHLKNSGRSR"
misc_feature complement(278827..279099)
/gene="insA"
/locus_tag="b0265"
/gene_synonym="ECK4521"
/note="Transposase and inactivated derivatives [DNA
replication, recombination, and repair]; Region: COG3677"
/db_xref="CDD:226202"
misc_feature complement(278992..279099)
/gene="insA"
/locus_tag="b0265"
/gene_synonym="ECK4521"
/note="InsA N-terminal domain; Region: Zn_Tnp_IS1;
pfam03811"
/db_xref="CDD:190760"
misc_feature complement(278836..278985)
/gene="insA"
/locus_tag="b0265"
/gene_synonym="ECK4521"
/note="InsA C-terminal domain; Region: HTH_Tnp_IS1;
pfam12759"
/db_xref="CDD:193235"
mobile_element 279155..279335
/mobile_element_type="insertion sequence:IS30B"
gene 279155..279328
/gene="insI1"
/locus_tag="b4708"
/gene_synonym="ECK0258"
/pseudo
/db_xref="EcoGene:EG40009"
/db_xref="GeneID:14678508"
CDS 279155..279328
/gene="insI1"
/locus_tag="b4708"
/gene_synonym="ECK0258"
/note="IS30 transposase;IS, phage, Tn; Transposon-related
functions; extrachromosomal; transposon related"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="EcoGene:EG40009"
/db_xref="GeneID:14678508"
mobile_element complement(279338..279649)
/mobile_element_type="insertion sequence:ISX"
gene complement(279338..279586)
/gene="insX"
/locus_tag="b4505"
/gene_synonym="ECK4473; ykgN"
/pseudo
/db_xref="EcoGene:EG14418"
/db_xref="GeneID:1450237"
CDS complement(279338..279586)
/gene="insX"
/locus_tag="b4505"
/gene_synonym="ECK4473; ykgN"
/note="pseudogene, predicted IS3 family transposase"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="ASAP:ABE-0285026"
/db_xref="UniProtKB/Swiss-Prot:Q79E92"
/db_xref="EcoGene:EG14418"
/db_xref="GeneID:1450237"
gene complement(279651..279959)
/gene="yagB"
/locus_tag="b0266"
/gene_synonym="ECK0267; JW0259"
/pseudo
/db_xref="EcoGene:EG12339"
/db_xref="GeneID:944944"
CDS complement(279651..279959)
/gene="yagB"
/locus_tag="b0266"
/gene_synonym="ECK0267; JW0259"
/note="pseudogene, CP4-6 prophage;Phage or Prophage
Related"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="ASAP:ABE-0000912"
/db_xref="UniProtKB/Swiss-Prot:P37008"
/db_xref="EcoGene:EG12339"
/db_xref="GeneID:944944"
gene complement(280053..281207)
/gene="yagA"
/locus_tag="b0267"
/gene_synonym="ECK0268; JW0260"
/db_xref="EcoGene:EG12338"
/db_xref="GeneID:944937"
CDS complement(280053..281207)
/gene="yagA"
/locus_tag="b0267"
/gene_synonym="ECK0268; JW0260"
/function="Phage or Prophage Related"
/codon_start=1
/transl_table=11
/product="CP4-6 prophage; predicted DNA-binding
transcriptional regulator"
/protein_id="NP_414801.1"
/db_xref="GI:16128252"
/db_xref="ASAP:ABE-0000915"
/db_xref="UniProtKB/Swiss-Prot:P37007"
/db_xref="EcoGene:EG12338"
/db_xref="GeneID:944937"
/translation="MESLMPWDARDTMSLRTEFVLFASQDGANIRSLCRRFGISPATG
YKWLQRWAQEGAAGLQDRPRIPHHSPNRSSDDITALLRMAHDRHERWGARKIKRWLED
QGHTMPAFSTVHNLMARHGLLPGASPGIPATGRFEHDAPNRLWQMDFKGHFPFGGGRC
HPLTLLDDHSRFSLCLAHCTDERRETVQQQLVSVFERYGLPDRMTMDNGSPWGDTTGT
WTALELWLMRLGIRVGHSRPYHPQTQGKLERFHRSLKAEVLQGKWFADSGELQRAFDH
WRTVYNLERPHEALDMAVPGSRYQPSARQYSGNTTPPEYDEGVMVRKVDISGKLSVKG
VSLSAGKAFRGERVGLKEMQEDGSYEVWWYSTKVGVIDLKKKSITMGKGC"
misc_feature complement(281016..281150)
/gene="yagA"
/locus_tag="b0267"
/gene_synonym="ECK0268; JW0260"
/note="Helix-turn-helix domain; Region: HTH_28; pfam13518"
/db_xref="CDD:205696"
misc_feature complement(280845..281132)
/gene="yagA"
/locus_tag="b0267"
/gene_synonym="ECK0268; JW0260"
/note="Winged helix-turn helix; Region: HTH_29; pfam13551"
/db_xref="CDD:205729"
misc_feature complement(280857..281081)
/gene="yagA"
/locus_tag="b0267"
/gene_synonym="ECK0268; JW0260"
/note="Homeodomain-like domain; Region: HTH_32; pfam13565"
/db_xref="CDD:205743"
misc_feature complement(280293..281006)
/gene="yagA"
/locus_tag="b0267"
/gene_synonym="ECK0268; JW0260"
/note="Transposase and inactivated derivatives [DNA
replication, recombination, and repair]; Region: Tra5;
COG2801"
/db_xref="CDD:32633"
misc_feature complement(280434..280787)
/gene="yagA"
/locus_tag="b0267"
/gene_synonym="ECK0268; JW0260"
/note="Integrase core domain; Region: rve; pfam00665"
/db_xref="CDD:201381"
misc_feature complement(280323..280523)
/gene="yagA"
/locus_tag="b0267"
/gene_synonym="ECK0268; JW0260"
/note="Integrase core domain; Region: rve_3; pfam13683"
/db_xref="CDD:205859"
gene 281502..282410
/gene="yagE"
/locus_tag="b0268"
/gene_synonym="ECK0269; JW0261"
/db_xref="EcoGene:EG13344"
/db_xref="GeneID:944925"
CDS 281502..282410
/gene="yagE"
/locus_tag="b0268"
/gene_synonym="ECK0269; JW0261"
/function="Phage or Prophage Related"
/note="putative lyase/synthase"
/codon_start=1
/transl_table=11
/product="2-keto-3-deoxy gluconate (KDG) aldolase; CP4-6
prophage"
/protein_id="NP_414802.2"
/db_xref="GI:345452715"
/db_xref="ASAP:ABE-0000921"
/db_xref="UniProtKB/Swiss-Prot:P75682"
/db_xref="EcoGene:EG13344"
/db_xref="GeneID:944925"
/translation="MPQSALFTGIIPPVSTIFTADGQLDKPGTAALIDDLIKAGVDGL
FFLGSGGEFSQLGAEERKAIARFAIDHVDRRVPVLIGTGGTNARETIELSQHAQQAGA
DGIVVINPYYWKVSEANLIRYFEQVADSVTLPVMLYNFPALTGQDLTPALVKTLADSR
SNIIGIKDTIDSVAHLRSMIHTVKGAHPHFTVLCGYDDHLFNTLLLGGDGAISASGNF
APQVSVNLLKAWRDGDVAKAAGYHQTLLQIPQMYQLDTPFVNVIKEAIVLCGRPVSTH
VLPPASPLDEPRKAQLKTLLQQLKLC"
misc_feature 281508..282362
/gene="yagE"
/locus_tag="b0268"
/gene_synonym="ECK0269; JW0261"
/note="Dihydrodipicolinate synthase/N-acetylneuraminate
lyase [Amino acid transport and metabolism / Cell envelope
biogenesis, outer membrane]; Region: DapA; COG0329"
/db_xref="CDD:30677"
misc_feature 281529..282359
/gene="yagE"
/locus_tag="b0268"
/gene_synonym="ECK0269; JW0261"
/note="Dihydrodipicolinate synthase family; Region:
DHDPS-like; cd00408"
/db_xref="CDD:188630"
misc_feature order(281538..281540,281634..281636,281643..281651,
281913..281915,282000..282002)
/gene="yagE"
/locus_tag="b0268"
/gene_synonym="ECK0269; JW0261"
/note="inhibitor site; inhibition site"
/db_xref="CDD:188630"
misc_feature order(281634..281636,281643..281651,281913..281915,
282000..282002,282135..282137)
/gene="yagE"
/locus_tag="b0268"
/gene_synonym="ECK0269; JW0261"
/note="active site"
/db_xref="CDD:188630"
misc_feature order(281646..281648,281661..281666,281757..281762,
281832..281837,281856..281858,281868..281870,
282336..282341)
/gene="yagE"
/locus_tag="b0268"
/gene_synonym="ECK0269; JW0261"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:188630"
misc_feature 282000..282002
/gene="yagE"
/locus_tag="b0268"
/gene_synonym="ECK0269; JW0261"
/note="catalytic residue [active]"
/db_xref="CDD:188630"
gene 282425..284392
/gene="yagF"
/locus_tag="b0269"
/gene_synonym="ECK0270; JW0262"
/db_xref="EcoGene:EG13345"
/db_xref="GeneID:944928"
CDS 282425..284392
/gene="yagF"
/locus_tag="b0269"
/gene_synonym="ECK0270; JW0262"
/function="Phage or Prophage Related"
/note="putative dehydratase"
/codon_start=1
/transl_table=11
/product="CP4-6 prophage; predicted dehydratase"
/protein_id="NP_414803.1"
/db_xref="GI:16128254"
/db_xref="ASAP:ABE-0000923"
/db_xref="UniProtKB/Swiss-Prot:P77596"
/db_xref="EcoGene:EG13345"
/db_xref="GeneID:944928"
/translation="MTIEKIFTPQDDAFYAVITHAAGPQGALPLTPQMLMESPSGNLF
GMTQNAGMGWDANKLTGKEVLIIGTQGGIRAGDGRPIALGYHTGHWEIGMQMQAAAKE
ITRNGGIPFAAFVSDPCDGRSQGTHGMFDSLPYRNDAAIVFRRLIRSLPTRRAVIGVA
TCDKGLPATMIALAAMHDLPTILVPGGATLPPTVGEDAGKVQTIGARFANHELSLQEA
AELGCRACASPGGGCQFLGTAGTSQVVAEALGLALPHSALAPSGQAVWLEIARQSARA
VSELDSRGITTRDILSDKAIENAMVIHAAFGGSTNLLLHIPAIAHAAGCTIPDVEHWT
RINRKVPRLVSVLPNGPDYHPTVRAFLAGGVPEVMLHLRDLGLLHLDAMTVTGQTVGE
NLEWWQASERRARFRQCLREQDGVEPDDVILPPEKAKAKGLTSTVCFPTGNIAPEGSV
IKATAIDPSVVGEDGVYHHTGRVRVFVSEAQAIKAIKREEIVQGDIMVVIGGGPSGTG
MEETYQLTSALKHISWGKTVSLITDARFSGVSTGACFGHVSPEALAGGPIGKLRDNDI
IEIAVDRLTLTGSVNFIGTADNPLTPEEGARELARRQTHPDLHAHDFLPDDTRLWAAL
QSVSGGTWKGCIYDTDKIIEVINAGKKALGI"
misc_feature 282425..284386
/gene="yagF"
/locus_tag="b0269"
/gene_synonym="ECK0270; JW0262"
/note="putative dehydratase; Provisional; Region:
PRK08211"
/db_xref="CDD:236189"
misc_feature 282482..284350
/gene="yagF"
/locus_tag="b0269"
/gene_synonym="ECK0270; JW0262"
/note="Dihydroxyacid dehydratase/phosphogluconate
dehydratase [Amino acid transport and metabolism /
Carbohydrate transport and metabolism]; Region: IlvD;
COG0129"
/db_xref="CDD:223207"
gene 284619..286001
/gene="yagG"
/locus_tag="b0270"
/gene_synonym="ECK0271; JW0263"
/db_xref="EcoGene:EG13346"
/db_xref="GeneID:944947"
CDS 284619..286001
/gene="yagG"
/locus_tag="b0270"
/gene_synonym="ECK0271; JW0263"
/function="Phage or Prophage Related"
/note="putative permease"
/codon_start=1
/transl_table=11
/product="CP4-6 prophage; predicted sugar transporter"
/protein_id="NP_414804.1"
/db_xref="GI:16128255"
/db_xref="ASAP:ABE-0000929"
/db_xref="UniProtKB/Swiss-Prot:P75683"
/db_xref="EcoGene:EG13346"
/db_xref="GeneID:944947"
/translation="MTQLTMKDKIGYGLGDTACGFVWQATMFLLAYFYTDVFGLSAGI
MGTLFLVSRVLDAVTDPLMGLLVDRTRTRHGQFRPFLLWGAIPFGIVCVLTFYTPDFS
AQGKIIYACVTYILLTLVYTFVNVPYCAMPGVITADPKERHALQSWRFFLAAAGSLAI
SGIALPLVSIIGKGDEQVGYFGAMCVLGLSGVVLLYVCFFTTKERYTFEVQPGSSVAK
DLKLLLGNSQWRIMCAFKMMATCSNVVRGGATLYFVKYVMDHPELATQFLLYGSLATM
FGSLCSSRLLGRFDRVTAFKWIIVAYSLISLLIFVTPAEHIALIFALNILFLFVFNTT
TPLQWLMASDVVDYEESRSGRRLDGLVFSTYLFSLKIGLAIGGAVVGWILAYVNYSAS
SSVQPVEVLTTIKILFCVVPVVLYAGMFIMLSLYKLTDARVEAISRQLIKHRAAQGEA
VPDAATAASH"
misc_feature 284619..285944
/gene="yagG"
/locus_tag="b0270"
/gene_synonym="ECK0271; JW0263"
/note="putative symporter YagG; Provisional; Region:
PRK09669"
/db_xref="CDD:236610"
misc_feature 284628..285866
/gene="yagG"
/locus_tag="b0270"
/gene_synonym="ECK0271; JW0263"
/note="melibiose:sodium symporter; Provisional; Region:
PRK10429; cl15392"
/db_xref="CDD:199528"
gene 286013..287623
/gene="yagH"
/locus_tag="b0271"
/gene_synonym="ECK0272; JW0264"
/db_xref="EcoGene:EG13347"
/db_xref="GeneID:944949"
CDS 286013..287623
/gene="yagH"
/locus_tag="b0271"
/gene_synonym="ECK0272; JW0264"
/function="Phage or Prophage Related"
/note="putative beta-xylosidase"
/codon_start=1
/transl_table=11
/product="CP4-6 prophage; predicted
xylosidase/arabinosidase"
/protein_id="NP_414805.1"
/db_xref="GI:16128256"
/db_xref="ASAP:ABE-0000931"
/db_xref="UniProtKB/Swiss-Prot:P77713"
/db_xref="EcoGene:EG13347"
/db_xref="GeneID:944949"
/translation="MEITNPILTGFNPDPSLCRQGEDYYIATSTFEWFPGVRIYHSRD
LKNWSLVSTPLDRVSMLDMKGNPDSGGIWAPCLSYADGKFWLLYTDVKIVDSPWKNGR
NFLVTAPSIEGPWSEPIPMGNGGFDPSLFHDDDGRKYYIYRPWGPRHHSNPHNTIVLQ
AFDPQTGTLSPERKTLFTGTPLCYTEGAHLYRHAGWYYLMAAEGGTSYEHAVVVLRSK
NIDGPYELHPDVTMMTSWHLPENPLQKSGHGSLLQTHTGEWYMAYLTSRPLRLPGVPL
LASGGRGYCPLGRETGIARIEWRDGWPYVEGGKHAQLTVKGPQVAEQPAAVPGNWRDD
FDASSLDPELQTLRIPFDDTLGSLTARPGFLRLYGNDSLNSTFTQSTVARRWQHFAFR
AETRMEFSPVHFQQSAGLTCYYNSKNWSYCFVDYEEGQGRTIKVIQLDHNVPSWPLHE
QPIPVPEHAESVWLRVDVDTLVYRYSYSFDGETWHTVPVTYEAWKLSDDYIGGRGFFT
GAFVGLHCEDISGDGCYADFDYFTYEPV"
misc_feature 286013..287620
/gene="yagH"
/locus_tag="b0271"
/gene_synonym="ECK0272; JW0264"
/note="Beta-xylosidase [Carbohydrate transport and
metabolism]; Region: XynB; COG3507"
/db_xref="CDD:33310"
misc_feature 286025..286927
/gene="yagH"
/locus_tag="b0271"
/gene_synonym="ECK0272; JW0264"
/note="Glycosyl hydrolase family 43, beta-D-xylosidase;
Region: GH43_XYL_1; cd09000"
/db_xref="CDD:185741"
misc_feature order(286052..286054,286103..286105,286229..286234,
286388..286393,286568..286570,286625..286630,
286754..286756,286877..286879)
/gene="yagH"
/locus_tag="b0271"
/gene_synonym="ECK0272; JW0264"
/note="inhibitor binding site; inhibition site"
/db_xref="CDD:185741"
misc_feature order(286052..286054,286391..286393,286568..286570)
/gene="yagH"
/locus_tag="b0271"
/gene_synonym="ECK0272; JW0264"
/note="active site"
/db_xref="CDD:185741"
misc_feature <287180..287617
/gene="yagH"
/locus_tag="b0271"
/gene_synonym="ECK0272; JW0264"
/note="Protein of unknown function (DUF1349); Region:
DUF1349; cl01397"
/db_xref="CDD:207397"
gene complement(287628..288386)
/gene="yagI"
/locus_tag="b0272"
/gene_synonym="ECK0273; JW0265"
/db_xref="EcoGene:EG13349"
/db_xref="GeneID:945016"
CDS complement(287628..288386)
/gene="yagI"
/locus_tag="b0272"
/gene_synonym="ECK0273; JW0265"
/function="Phage or Prophage Related"
/GO_function="GO:0016564 - transcription repressor
activity"
/GO_process="GO:0006350 - transcription"
/note="putative regulator"
/codon_start=1
/transl_table=11
/product="CP4-6 prophage; predicted DNA-binding
transcriptional regulator"
/protein_id="NP_414806.1"
/db_xref="GI:16128257"
/db_xref="ASAP:ABE-0000933"
/db_xref="UniProtKB/Swiss-Prot:P77300"
/db_xref="EcoGene:EG13349"
/db_xref="GeneID:945016"
/translation="MPIIQSVERALQILDLFNEQATELKITDISKLMGLSKSTLHSLL
KTLQLHGYIDQNPENGKYRLGMKLVERGHFVVGSIDIRQKAKGWLTELSRRTGQTTHL
GILDGREGVYIEKIEGKLAAIAYSRIGRRLPVHATAIGKVLIAWLGEAELNALLEGYQ
YTTFTPATLASREALMSALAQTREQGYALDSEENEQGVRCVAVPVWNHESRVIAALSL
STLTSRVDDAELANFREQLQQAGLALSRALGYPA"
misc_feature complement(288105..288377)
/gene="yagI"
/locus_tag="b0272"
/gene_synonym="ECK0273; JW0265"
/note="helix_turn_helix isocitrate lyase regulation;
Region: HTH_ICLR; smart00346"
/db_xref="CDD:214629"
misc_feature complement(287637..288374)
/gene="yagI"
/locus_tag="b0272"
/gene_synonym="ECK0273; JW0265"
/note="Transcriptional regulator [Transcription]; Region:
IclR; COG1414"
/db_xref="CDD:224332"
misc_feature complement(287646..288017)
/gene="yagI"
/locus_tag="b0272"
/gene_synonym="ECK0273; JW0265"
/note="Bacterial transcriptional regulator; Region: IclR;
pfam01614"
/db_xref="CDD:201890"
gene complement(288525..289529)
/gene="argF"
/locus_tag="b0273"
/gene_synonym="Arg5; argD; ECK0274; JW0266"
/db_xref="EcoGene:EG10067"
/db_xref="GeneID:944844"
CDS complement(288525..289529)
/gene="argF"
/locus_tag="b0273"
/gene_synonym="Arg5; argD; ECK0274; JW0266"
/EC_number="2.1.3.3"
/function="enzyme; Amino acid biosynthesis: Arginine;
Phage or Prophage Related"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006526 - arginine biosynthetic process"
/note="ornithine carbamoyltransferase 2, chain F"
/codon_start=1
/transl_table=11
/product="ornithine carbamoyltransferase 2, chain F; CP4-6
prophage"
/protein_id="NP_414807.1"
/db_xref="GI:16128258"
/db_xref="ASAP:ABE-0000939"
/db_xref="UniProtKB/Swiss-Prot:P06960"
/db_xref="EcoGene:EG10067"
/db_xref="GeneID:944844"
/translation="MSDLYKKHFLKLLDFTPAQFTSLLTLAAQLKADKKNGKEVQKLT
GKNIALIFEKDSTRTRCSFEVAAFDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
GIQYRGHGQEVVETLAQYAGVPVWNGLTNEFHPTQLLADLMTMQEHLPGKAFNEMTLV
YAGDARNNMGNSMLEAAALTGLDLRLLAPKACWPEESLVAECSALAEKHGGKITLTED
VAAGVKGADFIYTDVWVSMGEAKEKWAERIALLRGYQVNAQMMALTDNPNVKFLHCLP
AFHDDQTTLGKQMAKEFDLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMMATLGE
"
misc_feature complement(288534..289529)
/gene="argF"
/locus_tag="b0273"
/gene_synonym="Arg5; argD; ECK0274; JW0266"
/note="ornithine carbamoyltransferase subunit I;
Provisional; Region: PRK03515"
/db_xref="CDD:179587"
mat_peptide complement(288528..289526)
/gene="argF"
/locus_tag="b0273"
/gene_synonym="Arg5; argD; ECK0274; JW0266"
/product="ornithine carbamoyltransferase 2, chain F; CP4-6
prophage"
/experiment="N-terminus verified by Edman degradation:
PMID 3968036"
misc_feature complement(289086..289511)
/gene="argF"
/locus_tag="b0273"
/gene_synonym="Arg5; argD; ECK0274; JW0266"
/note="Aspartate/ornithine carbamoyltransferase,
carbamoyl-P binding domain; Region: OTCace_N; pfam02729"
/db_xref="CDD:217204"
misc_feature complement(288546..289067)
/gene="argF"
/locus_tag="b0273"
/gene_synonym="Arg5; argD; ECK0274; JW0266"
/note="Aspartate/ornithine carbamoyltransferase, Asp/Orn
binding domain; Region: OTCace; pfam00185"
/db_xref="CDD:215776"
gene 289653..289857
/gene="ykgS"
/locus_tag="b4688"
/gene_synonym="ECK4504"
/pseudo
/db_xref="EcoGene:EG14492"
/db_xref="GeneID:7751632"
CDS 289653..289857
/gene="ykgS"
/locus_tag="b4688"
/gene_synonym="ECK4504"
/note="pseudogene"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="EcoGene:EG14492"
/db_xref="GeneID:7751632"
mobile_element complement(289858..290625)
/mobile_element_type="insertion sequence:IS1C"
gene complement(289873..290376)
/gene="insB1"
/locus_tag="b0274"
/gene_synonym="ECK0022"
/db_xref="EcoGene:EG40002"
/db_xref="GeneID:944950"
CDS complement(289873..290376)
/gene="insB1"
/locus_tag="b0274"
/gene_synonym="ECK0022"
/function="IS, phage, Tn; Transposon-related functions;
extrachromosomal; transposon related"
/codon_start=1
/transl_table=11
/product="IS1 transposase B"
/protein_id="NP_414808.1"
/db_xref="GI:16128259"
/db_xref="ASAP:ABE-0000948"
/db_xref="UniProtKB/Swiss-Prot:P0CF27"
/db_xref="EcoGene:EG40002"
/db_xref="GeneID:944950"
/translation="MPGNRPHYGRWPQHDFPPFKKLRPQSVTSRIQPGSDVIVCAEMD
EQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDG
WPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVEQHDKVIGHY
LNIKHYQ"
misc_feature complement(289876..290268)
/gene="insB1"
/locus_tag="b0274"
/gene_synonym="ECK0022"
/note="IS1 transposase; Region: DDE_Tnp_IS1; pfam03400"
/db_xref="CDD:112225"
gene complement(290295..290570)
/gene="insA"
/locus_tag="b0275"
/gene_synonym="ECK4523"
/note="insA3"
/db_xref="EcoGene:EG40001"
/db_xref="GeneID:944951"
CDS complement(290295..290570)
/gene="insA"
/locus_tag="b0275"
/gene_synonym="ECK4523"
/function="IS, phage, Tn; Transposon-related functions;
extrachromosomal; transposon related"
/codon_start=1
/transl_table=11
/product="IS1 repressor TnpA"
/protein_id="NP_414809.1"
/db_xref="GI:16128260"
/db_xref="ASAP:ABE-0000951"
/db_xref="UniProtKB/Swiss-Prot:P0CF09"
/db_xref="EcoGene:EG40001"
/db_xref="GeneID:944951"
/translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHLKNSGRSR"
misc_feature complement(290298..290570)
/gene="insA"
/locus_tag="b0275"
/gene_synonym="ECK4523"
/note="Transposase and inactivated derivatives [DNA
replication, recombination, and repair]; Region: COG3677"
/db_xref="CDD:226202"
misc_feature complement(290463..290570)
/gene="insA"
/locus_tag="b0275"
/gene_synonym="ECK4523"
/note="InsA N-terminal domain; Region: Zn_Tnp_IS1;
pfam03811"
/db_xref="CDD:190760"
misc_feature complement(290307..290456)
/gene="insA"
/locus_tag="b0275"
/gene_synonym="ECK4523"
/note="InsA C-terminal domain; Region: HTH_Tnp_IS1;
pfam12759"
/db_xref="CDD:193235"
gene 290628..291455
/gene="yagJ"
/locus_tag="b0276"
/gene_synonym="ECK0275; JW0270"
/pseudo
/db_xref="EcoGene:EG13549"
/db_xref="GeneID:949094"
CDS 290628..291455
/gene="yagJ"
/locus_tag="b0276"
/gene_synonym="ECK0275; JW0270"
/note="CP4-6 prophage; putative protein;Phage or Prophage
Related"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="ASAP:ABE-0000963"
/db_xref="UniProtKB/Swiss-Prot:P77169"
/db_xref="EcoGene:EG13549"
/db_xref="GeneID:949094"
gene complement(291546..292172)
/gene="yagK"
/locus_tag="b0277"
/gene_synonym="ECK0276; JW0271"
/db_xref="EcoGene:EG13550"
/db_xref="GeneID:945847"
CDS complement(291546..292172)
/gene="yagK"
/locus_tag="b0277"
/gene_synonym="ECK0276; JW0271"
/function="Phage or Prophage Related"
/codon_start=1
/transl_table=11
/product="CP4-6 prophage; conserved protein"
/protein_id="NP_414811.1"
/db_xref="GI:16128262"
/db_xref="ASAP:ABE-0000965"
/db_xref="UniProtKB/Swiss-Prot:P77657"
/db_xref="EcoGene:EG13550"
/db_xref="GeneID:945847"
/translation="MKTYQGTHGVHILEYQSKINKLLCYLTNRYPRLIAVRVDLHYPK
IVDNGDNICCFPNLEPGVISRMRESLRAKLEADRTRKVREDKRIYRCPLFIIWAKEYS
ESGKCHYHICLLFNKDAYYHLGDYDQDDNLRGMITGAWYSALRLERDDHPGLVHFPEN
CKYVLDTNSTDFQQNYQALLTRLDYLTKVESKVFGEGDRNFGCSQIEL"
misc_feature complement(291558..292121)
/gene="yagK"
/locus_tag="b0277"
/gene_synonym="ECK0276; JW0271"
/note="Protein of unknown function (DUF3296); Region:
DUF3296; pfam11726"
/db_xref="CDD:152162"
gene complement(292444..293142)
/gene="yagL"
/locus_tag="b0278"
/gene_synonym="ECK0277; JW0272"
/db_xref="EcoGene:EG13551"
/db_xref="GeneID:945845"
CDS complement(292444..293142)
/gene="yagL"
/locus_tag="b0278"
/gene_synonym="ECK0277; JW0272"
/function="Phage or Prophage Related"
/note="DNA-binding protein"
/codon_start=1
/transl_table=11
/product="CP4-6 prophage; DNA-binding protein"
/protein_id="NP_414812.1"
/db_xref="GI:16128263"
/db_xref="ASAP:ABE-0000970"
/db_xref="UniProtKB/Swiss-Prot:P77607"
/db_xref="EcoGene:EG13551"
/db_xref="GeneID:945845"
/translation="MRGKILLYQLKYRWQSLSIFGCFLCKMTLFRYQKIIYDTGVHQM
RSFFYTICSSEQQESITDHHSLAEICQKFNILPEHVVIEQVDIKEVVSEQRLLRQLIH
HEMNRQDTLVIPDLSCLGRTVEDLQNILFFCLQKEMFIYSYHPASRIEPSAESCLSFL
IARQDTIDIHNLKSTKSRYRHVKKKLGRKEGSKYRRDITILKKGGFTQAEIAKKLSIS
LSTVKRHWNNGIIG"
misc_feature complement(292450..293139)
/gene="yagL"
/locus_tag="b0278"
/gene_synonym="ECK0277; JW0272"
/note="Site-specific recombinases, DNA invertase Pin
homologs [DNA replication, recombination, and repair];
Region: PinR; COG1961"
/db_xref="CDD:32144"
misc_feature complement(<292468..292560)
/gene="yagL"
/locus_tag="b0278"
/gene_synonym="ECK0277; JW0272"
/note="Helix-turn-helix domains; Region: HTH; cl00088"
/db_xref="CDD:214025"
gene complement(293169..294023)
/gene="yagM"
/locus_tag="b0279"
/gene_synonym="ECK0278; JW0273"
/db_xref="EcoGene:EG13552"
/db_xref="GeneID:944959"
CDS complement(293169..294023)
/gene="yagM"
/locus_tag="b0279"
/gene_synonym="ECK0278; JW0273"
/function="Phage or Prophage Related"
/codon_start=1
/transl_table=11
/product="CP4-6 prophage; putative protein"
/protein_id="NP_414813.1"
/db_xref="GI:16128264"
/db_xref="ASAP:ABE-0000972"
/db_xref="UniProtKB/Swiss-Prot:P71296"
/db_xref="EcoGene:EG13552"
/db_xref="GeneID:944959"
/translation="MSNSVTNFEMSSVLPGKKPCQGKNNESQVVQTTPIKKHSVTFKN
QSSLGVIDHYARLTNKSHSSVIAEVVDLAIPILEKCNRHNWSINEIKNDLLKFSIKES
INRSRGKTEVTLEEYCSLIWKTNIMSPLKIPIADYFQLNANDEFMGKDEKTVIRERLS
SLRENYDMEKAIYIYNQRHFDVKHQSVSGYSNIILIHRTTFEGYYFDAGQALLLSTSQ
LIIFGINEVLRRKGIVMPYPVVCWIDIYHVNEMVVMLPVLRKTDVSNRVNVPDDIIIN
PYSQESRT"
gene complement(294363..294803)
/gene="yagN"
/locus_tag="b0280"
/gene_synonym="ECK0279; JW0274"
/db_xref="EcoGene:EG13553"
/db_xref="GeneID:945349"
CDS complement(294363..294803)
/gene="yagN"
/locus_tag="b0280"
/gene_synonym="ECK0279; JW0274"
/function="Phage or Prophage Related"
/codon_start=1
/transl_table=11
/product="CP4-6 prophage; putative protein"
/protein_id="NP_414814.1"
/db_xref="GI:16128265"
/db_xref="ASAP:ABE-0000976"
/db_xref="UniProtKB/Swiss-Prot:P71297"
/db_xref="EcoGene:EG13553"
/db_xref="GeneID:945349"
/translation="MATPATVSIEPTLAAIRARWCINSSKTTQSFNDPASMEEVVEYL
KGTYSALRKSVACAKLKILHLKQRMQNATNFLARLMSCKNQASRSHHSTAKSAKSALS
SDSGDGSDPDPEPETFPSAFITTPTNSIMLKAFFANISITEVAK"
gene complement(294920..296320)
/gene="intF"
/locus_tag="b0281"
/gene_synonym="ECK0280; JW0275; yagO"
/db_xref="EcoGene:EG13554"
/db_xref="GeneID:947351"
CDS complement(294920..296320)
/gene="intF"
/locus_tag="b0281"
/gene_synonym="ECK0280; JW0275; yagO"
/function="Phage or Prophage Related"
/note="putative phage integrase"
/codon_start=1
/transl_table=11
/product="CP4-6 prophage; predicted phage integrase"
/protein_id="NP_414815.1"
/db_xref="GI:16128266"
/db_xref="ASAP:ABE-0000979"
/db_xref="UniProtKB/Swiss-Prot:P71298"
/db_xref="EcoGene:EG13554"
/db_xref="GeneID:947351"
/translation="MFIPSIYLHQQLHYCKTAILNWSRKMALSRQKFTFERLRRFTLP
EGKKQTFLWDADVTTLACRATSGAKAFVFQSVYAGKTLRMTIGNINDWKIDDARAEAR
RLQTLIDTGIDPRIAKAVKIAEAESLQAESRKTKVTFSVAWEDYLQELRTGISAKTKR
PYSTRYIADHINLSSRGGESKKRGQGPTSAGPLASLLNLPLSELTPDYIAAWLSTERQ
NRPTVTAHAYRLLRAFIKWSNYQKKYQGIIPGDLAQDYNVRKMVPVSASKADDCLQKE
QLKSWFSAVRSLNNPIASAYLQVLLLTGARREEIASLRWSDVDFKWSSMRIKDKIEGE
RIIPLTPYVSELLNVLAQSPNSDVNKEGWVFRSNSKSGKIIEPRSAHNRALVLAELPH
ISLHGLRRSFGTLAEWVEVPTGIVAQIMGHKPSALAEKHYRRRPLDLLRKWHEKIETW
ILNEAGITIKNNVDMR"
misc_feature complement(294971..296128)
/gene="intF"
/locus_tag="b0281"
/gene_synonym="ECK0280; JW0275; yagO"
/note="DNA breaking-rejoining enzymes, C-terminal
catalytic domain. The DNA breaking-rejoining enzyme
superfamily includes type IB topoisomerases and tyrosine
recombinases that share the same fold in their catalytic
domain containing six conserved active site...; Region:
DNA_BRE_C; cl00213"
/db_xref="CDD:211431"
misc_feature complement(order(295025..295027,295133..295141,
295331..295333,295400..295405))
/gene="intF"
/locus_tag="b0281"
/gene_synonym="ECK0280; JW0275; yagO"
/note="DNA binding site [nucleotide binding]"
/db_xref="CDD:29495"
misc_feature complement(order(295025..295027,295055..295057,
295124..295126,295133..295135,295331..295333,
295403..295405))
/gene="intF"
/locus_tag="b0281"
/gene_synonym="ECK0280; JW0275; yagO"
/note="Int/Topo IB signature motif; other site"
/db_xref="CDD:29495"
misc_feature complement(order(295025..295027,295055..295057,
295124..295126,295133..295135,295403..295405))
/gene="intF"
/locus_tag="b0281"
/gene_synonym="ECK0280; JW0275; yagO"
/note="active site"
/db_xref="CDD:29495"
gene 296430..296478
/gene="ptwF"
/locus_tag="b4629"
/gene_synonym="ECK4462"
/pseudo
/db_xref="EcoGene:EG31171"
/db_xref="GeneID:5625555"
tRNA 296430..296478
/gene="ptwF"
/locus_tag="b4629"
/gene_synonym="ECK4462"
/product="tRNA-OTHER"
/pseudo
/db_xref="EcoGene:EG31171"
/db_xref="GeneID:5625555"
gene complement(296605..296943)
/gene="yagP"
/locus_tag="b4694"
/gene_synonym="ECK0281; JW0276"
/pseudo
/db_xref="EcoGene:EG13555"
/db_xref="GeneID:7751633"
CDS complement(296605..296943)
/gene="yagP"
/locus_tag="b4694"
/gene_synonym="ECK0281; JW0276"
/GO_process="GO:0006355 - regulation of transcription,
DNA-dependent"
/note="pseudogene, LysR family, fragment;putative
regulator; Not classified;
putative transcriptional regulator LYSR-type"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="ASAP:ABE-0000982"
/db_xref="UniProtKB/Swiss-Prot:P75684"
/db_xref="EcoGene:EG13555"
/db_xref="GeneID:7751633"
gene complement(296938..297015)
/gene="ykgT"
/locus_tag="b4695"
/gene_synonym="ECK4508; yagP"
/pseudo
/db_xref="EcoGene:EG14496"
/db_xref="GeneID:7751634"
CDS complement(296938..297015)
/gene="ykgT"
/locus_tag="b4695"
/gene_synonym="ECK4508; yagP"
/note="pseudogene, MobA-related"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="EcoGene:EG14496"
/db_xref="GeneID:7751634"
gene complement(296994..297950)
/gene="paoD"
/locus_tag="b0283"
/gene_synonym="ECK0282; JW0277; yagQ"
/db_xref="EcoGene:EG13556"
/db_xref="GeneID:945010"
CDS complement(296994..297950)
/gene="paoD"
/locus_tag="b0283"
/gene_synonym="ECK0282; JW0277; yagQ"
/codon_start=1
/transl_table=11
/product="moco insertion factor for PaoABC aldehyde
oxidoreductase"
/protein_id="NP_414817.1"
/db_xref="GI:16128268"
/db_xref="ASAP:ABE-0000984"
/db_xref="UniProtKB/Swiss-Prot:P77183"
/db_xref="EcoGene:EG13556"
/db_xref="GeneID:945010"
/translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPVGLRYDPQAQSLVCLPTQ
TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETTQ
IRAPVGIFPKARDAHTLALSVLAEVASVRLHQEEDSCLPPSS"
misc_feature complement(297018..297878)
/gene="paoD"
/locus_tag="b0283"
/gene_synonym="ECK0282; JW0277; yagQ"
/note="Xanthine and CO dehydrogenases maturation factor,
XdhC/CoxF family [Posttranslational modification, protein
turnover, chaperones]; Region: XdhC; COG1975"
/db_xref="CDD:224886"
misc_feature complement(297648..297857)
/gene="paoD"
/locus_tag="b0283"
/gene_synonym="ECK0282; JW0277; yagQ"
/note="XdhC and CoxI family; Region: XdhC_CoxI; pfam02625"
/db_xref="CDD:202317"
misc_feature complement(297048..297413)
/gene="paoD"
/locus_tag="b0283"
/gene_synonym="ECK0282; JW0277; yagQ"
/note="XdhC Rossmann domain; Region: XdhC_C; pfam13478"
/db_xref="CDD:222162"
gene complement(297960..300158)
/gene="paoC"
/locus_tag="b0284"
/gene_synonym="ECK0283; hsm; JW0278; yagR"
/db_xref="EcoGene:EG13557"
/db_xref="GeneID:944961"
CDS complement(297960..300158)
/gene="paoC"
/locus_tag="b0284"
/gene_synonym="ECK0283; hsm; JW0278; yagR"
/codon_start=1
/transl_table=11
/product="PaoABC aldehyde oxidoreductase, Moco-containing
subunit"
/protein_id="NP_414818.1"
/db_xref="GI:16128269"
/db_xref="ASAP:ABE-0000988"
/db_xref="UniProtKB/Swiss-Prot:P77489"
/db_xref="EcoGene:EG13557"
/db_xref="GeneID:944961"
/translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
TRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
VHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
QWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEATAGGRL
TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
misc_feature complement(297999..300122)
/gene="paoC"
/locus_tag="b0284"
/gene_synonym="ECK0283; hsm; JW0278; yagR"
/note="Aerobic-type carbon monoxide dehydrogenase, large
subunit CoxL/CutL homologs [Energy production and
conversion]; Region: CoxL; COG1529"
/db_xref="CDD:224446"
misc_feature complement(299745..300059)
/gene="paoC"
/locus_tag="b0284"
/gene_synonym="ECK0283; hsm; JW0278; yagR"
/note="Aldehyde oxidase and xanthine dehydrogenase, a/b
hammerhead domain; Region: Ald_Xan_dh_C; pfam01315"
/db_xref="CDD:216430"
misc_feature complement(298155..299714)
/gene="paoC"
/locus_tag="b0284"
/gene_synonym="ECK0283; hsm; JW0278; yagR"
/note="Molybdopterin-binding domain of aldehyde
dehydrogenase; Region: Ald_Xan_dh_C2; pfam02738"
/db_xref="CDD:217210"
gene complement(300155..301111)
/gene="paoB"
/locus_tag="b0285"
/gene_synonym="ECK0284; JW0279; yagS"
/db_xref="EcoGene:EG13558"
/db_xref="GeneID:945710"
CDS complement(300155..301111)
/gene="paoB"
/locus_tag="b0285"
/gene_synonym="ECK0284; JW0279; yagS"
/codon_start=1
/transl_table=11
/product="PaoABC aldehyde oxidoreductase, FAD-containing
subunit"
/protein_id="NP_414819.1"
/db_xref="GI:16128270"
/db_xref="ASAP:ABE-0000990"
/db_xref="UniProtKB/Swiss-Prot:P77324"
/db_xref="EcoGene:EG13558"
/db_xref="GeneID:945710"
/translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
QGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
misc_feature complement(300158..301111)
/gene="paoB"
/locus_tag="b0285"
/gene_synonym="ECK0284; JW0279; yagS"
/note="Aerobic-type carbon monoxide dehydrogenase, middle
subunit CoxM/CutM homologs [Energy production and
conversion]; Region: CoxM; COG1319"
/db_xref="CDD:224238"
misc_feature complement(300170..300430)
/gene="paoB"
/locus_tag="b0285"
/gene_synonym="ECK0284; JW0279; yagS"
/note="CO dehydrogenase flavoprotein C-terminal domain;
Region: CO_deh_flav_C; smart01092"
/db_xref="CDD:215021"
gene complement(301108..301797)
/gene="paoA"
/locus_tag="b0286"
/gene_synonym="ECK0285; JW0280; yagT"
/db_xref="EcoGene:EG13559"
/db_xref="GeneID:945330"
CDS complement(301108..301797)
/gene="paoA"
/locus_tag="b0286"
/gene_synonym="ECK0285; JW0280; yagT"
/function="putative enzyme; Not classified"
/note="putative xanthine dehydrogenase"
/codon_start=1
/transl_table=11
/product="PaoABC aldehyde oxidoreductase, 2Fe-2S subunit"
/protein_id="NP_414820.1"
/db_xref="GI:16128271"
/db_xref="ASAP:ABE-0000992"
/db_xref="UniProtKB/Swiss-Prot:P77165"
/db_xref="EcoGene:EG13559"
/db_xref="GeneID:945330"
/translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDTLRENLHLIGTKKGCDHG
QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
AIEDAAGEIKS"
misc_feature complement(301114..301764)
/gene="paoA"
/locus_tag="b0286"
/gene_synonym="ECK0285; JW0280; yagT"
/note="aldehyde oxidoreductase 2Fe-2S subunit;
Provisional; Region: PRK11433"
/db_xref="CDD:236910"
misc_feature complement(<301462..301605)
/gene="paoA"
/locus_tag="b0286"
/gene_synonym="ECK0285; JW0280; yagT"
/note="2Fe-2S iron-sulfur cluster binding domain; Region:
Fer2; pfam00111"
/db_xref="CDD:215725"
misc_feature complement(<301432..>301506)
/gene="paoA"
/locus_tag="b0286"
/gene_synonym="ECK0285; JW0280; yagT"
/note="2Fe-2S iron-sulfur cluster binding domain; Region:
Fer2_3; pfam13085"
/db_xref="CDD:221911"
misc_feature complement(301132..301401)
/gene="paoA"
/locus_tag="b0286"
/gene_synonym="ECK0285; JW0280; yagT"
/note="[2Fe-2S] binding domain; Region: Fer2_2; pfam01799"
/db_xref="CDD:201981"
gene 302215..302829
/gene="yagU"
/locus_tag="b0287"
/gene_synonym="ECK0286; JW0281"
/db_xref="EcoGene:EG13560"
/db_xref="GeneID:945677"
CDS 302215..302829
/gene="yagU"
/locus_tag="b0287"
/gene_synonym="ECK0286; JW0281"
/codon_start=1
/transl_table=11
/product="inner membrane protein, DUF1440 family"
/protein_id="NP_414821.1"
/db_xref="GI:16128272"
/db_xref="ASAP:ABE-0000995"
/db_xref="UniProtKB/Swiss-Prot:P0AAA1"
/db_xref="EcoGene:EG13560"
/db_xref="GeneID:945677"
/translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
misc_feature 302221..302814
/gene="yagU"
/locus_tag="b0287"
/gene_synonym="ECK0286; JW0281"
/note="Predicted periplasmic/secreted protein [Function
unknown]; Region: COG3477"
/db_xref="CDD:226008"
gene complement(303077..303406)
/gene="ykgJ"
/locus_tag="b0288"
/gene_synonym="ECK0287; JW0282"
/db_xref="EcoGene:EG14323"
/db_xref="GeneID:945905"
CDS complement(303077..303406)
/gene="ykgJ"
/locus_tag="b0288"
/gene_synonym="ECK0287; JW0282"
/function="putative carrier; Not classified"
/GO_function="GO:0009055 - electron carrier activity"
/note="putative ferredoxin"
/codon_start=1
/transl_table=11
/product="UPF0153 cysteine cluster protein, function
unknown"
/protein_id="NP_414822.1"
/db_xref="GI:16128273"
/db_xref="ASAP:ABE-0000997"
/db_xref="UniProtKB/Swiss-Prot:P0AAL9"
/db_xref="EcoGene:EG14323"
/db_xref="GeneID:945905"
/translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
KYGLTPL"
misc_feature complement(303080..303406)
/gene="ykgJ"
/locus_tag="b0288"
/gene_synonym="ECK0287; JW0282"
/note="Predicted Fe-S-cluster oxidoreductase [General
function prediction only]; Region: COG0727"
/db_xref="CDD:31071"
gene complement(303719..304429)
/gene="ecpE"
/locus_tag="b0289"
/gene_synonym="ECK0288; JW5030; matF; yagV"
/db_xref="EcoGene:EG13561"
/db_xref="GeneID:946631"
CDS complement(303719..304429)
/gene="ecpE"
/locus_tag="b0289"
/gene_synonym="ECK0288; JW5030; matF; yagV"
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="NP_414823.2"
/db_xref="GI:90111108"
/db_xref="ASAP:ABE-0001000"
/db_xref="UniProtKB/Swiss-Prot:P77263"
/db_xref="EcoGene:EG13561"
/db_xref="GeneID:946631"
/translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
YHGPRDNRERYYRVSFREVPTRNHTRRSPTGGVVSTEPVVVMDTILVVRPRQVQFKWS
FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
DKFIKISDSCPAKPPSAD"
gene complement(304398..306041)
/gene="ecpD"
/locus_tag="b0290"
/gene_synonym="ECK0289; JW0284; matE; yagW"
/db_xref="EcoGene:EG13562"
/db_xref="GeneID:947349"
CDS complement(304398..306041)
/gene="ecpD"
/locus_tag="b0290"
/gene_synonym="ECK0289; JW0284; matE; yagW"
/function="putative factor; Not classified"
/codon_start=1
/transl_table=11
/product="putative receptor"
/protein_id="NP_414824.1"
/db_xref="GI:16128275"
/db_xref="ASAP:ABE-0001002"
/db_xref="UniProtKB/Swiss-Prot:P77694"
/db_xref="EcoGene:EG13562"
/db_xref="GeneID:947349"
/translation="MRVNLLITMIIFALIWPVTALRAAVSKTTWADAPAREFVFVENN
SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
DAFYQYMQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
gene complement(306031..308556)
/gene="ecpC"
/locus_tag="b0291"
/gene_synonym="ECK0290; JW0285; matD; yagX"
/db_xref="EcoGene:EG13563"
/db_xref="GeneID:947606"
CDS complement(306031..308556)
/gene="ecpC"
/locus_tag="b0291"
/gene_synonym="ECK0290; JW0285; matD; yagX"
/function="putative enzyme; Not classified"
/note="putative enzyme"
/codon_start=1
/transl_table=11
/product="putative aromatic compound dioxygenase"
/protein_id="NP_414825.1"
/db_xref="GI:16128276"
/db_xref="ASAP:ABE-0001005"
/db_xref="UniProtKB/Swiss-Prot:P77802"
/db_xref="EcoGene:EG13563"
/db_xref="GeneID:947606"
/translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSRLTLYPGNVAVI
EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
misc_feature complement(306550..>307134)
/gene="ecpC"
/locus_tag="b0291"
/gene_synonym="ECK0290; JW0285; matD; yagX"
/note="Type VII secretion system (T7SS), usher protein;
Region: Usher; pfam00577"
/db_xref="CDD:216001"
misc_feature complement(<306127..306312)
/gene="ecpC"
/locus_tag="b0291"
/gene_synonym="ECK0290; JW0285; matD; yagX"
/note="Peptidase associated domain: C-terminal domain of
M14 N/E carboxypeptidase; putative folding, regulation, or
interaction domain; Region: Peptidase_M14NE-CP-C_like;
cl17499"
/db_xref="CDD:248053"
gene complement(308582..309250)
/gene="ecpB"
/locus_tag="b0292"
/gene_synonym="ECK0291; JW0286; matC; yagY"
/db_xref="EcoGene:EG13564"
/db_xref="GeneID:948806"
CDS complement(308582..309250)
/gene="ecpB"
/locus_tag="b0292"
/gene_synonym="ECK0291; JW0286; matC; yagY"
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="NP_414826.1"
/db_xref="GI:16128277"
/db_xref="ASAP:ABE-0001007"
/db_xref="UniProtKB/Swiss-Prot:P77188"
/db_xref="EcoGene:EG13564"
/db_xref="GeneID:948806"
/translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
IPVK"
misc_feature complement(<308726..309238)
/gene="ecpB"
/locus_tag="b0292"
/gene_synonym="ECK0291; JW0286; matC; yagY"
/note="putative fimbrial protein TcfA; Provisional;
Region: PRK15308"
/db_xref="CDD:237939"
gene complement(309308..309895)
/gene="ecpA"
/locus_tag="b0293"
/gene_synonym="ECK0292; JW0287; matB; yagZ"
/db_xref="EcoGene:EG13565"
/db_xref="GeneID:948759"
CDS complement(309308..309895)
/gene="ecpA"
/locus_tag="b0293"
/gene_synonym="ECK0292; JW0287; matB; yagZ"
/codon_start=1
/transl_table=11
/product="cryptic Mat fimbrillin gene"
/protein_id="NP_414827.1"
/db_xref="GI:16128278"
/db_xref="ASAP:ABE-0001009"
/db_xref="UniProtKB/Swiss-Prot:P0AAA3"
/db_xref="EcoGene:EG13565"
/db_xref="GeneID:948759"
/translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
mat_peptide complement(309311..309829)
/gene="ecpA"
/locus_tag="b0293"
/gene_synonym="ECK0292; JW0287; matB; yagZ"
/product="cryptic Mat fimbrillin gene"
/experiment="N-terminus verified by Edman degradation:
PMID 16522795"
gene complement(309970..310560)
/gene="ecpR"
/locus_tag="b0294"
/gene_synonym="ECK0293; JW5031; matA; ykgK"
/db_xref="EcoGene:EG14324"
/db_xref="GeneID:944966"
CDS complement(309970..310560)
/gene="ecpR"
/locus_tag="b0294"
/gene_synonym="ECK0293; JW5031; matA; ykgK"
/function="putative regulator; Not classified"
/codon_start=1
/transl_table=11
/product="putative regulator"
/protein_id="NP_414828.1"
/db_xref="GI:16128279"
/db_xref="ASAP:ABE-0001011"
/db_xref="UniProtKB/Swiss-Prot:P71301"
/db_xref="EcoGene:EG14324"
/db_xref="GeneID:944966"
/translation="MTWQSDYSRDYEVKNHMECQNRSDKYIWSPHDAYFYKGLSELIV
DIDRLIYLSLEKIRKDFVFINLSTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALA
NYWYYNSNIRGVVYAGLSRDIRKELVYVINGRFLRKDIKKDKITDREMEIIRMTAQGM
QPKSIARIENCSVKTVYTHRRNAEAKLYSKIYKLVQ"
misc_feature complement(310000..>310125)
/gene="ecpR"
/locus_tag="b0294"
/gene_synonym="ECK0293; JW5031; matA; ykgK"
/note="Response regulator containing a CheY-like receiver
domain and an HTH DNA-binding domain [Signal transduction
mechanisms / Transcription]; Region: CitB; COG2197"
/db_xref="CDD:32379"
misc_feature complement(<310000..310125)
/gene="ecpR"
/locus_tag="b0294"
/gene_synonym="ECK0293; JW5031; matA; ykgK"
/note="C-terminal DNA-binding domain of LuxR-like
proteins. This domain contains a helix-turn-helix motif
and binds DNA. Proteins belonging to this group are
response regulators; some act as transcriptional
activators, others as transcriptional repressors. Many...;
Region: LuxR_C_like; cd06170"
/db_xref="CDD:99777"
misc_feature complement(order(310018..310032,310036..310041,
310045..310050,310072..310080,310117..310125))
/gene="ecpR"
/locus_tag="b0294"
/gene_synonym="ECK0293; JW5031; matA; ykgK"
/note="DNA binding residues [nucleotide binding]"
/db_xref="CDD:99777"
gene 311336..311563
/gene="ykgL"
/locus_tag="b0295"
/gene_synonym="ECK0294; JW5033"
/db_xref="EcoGene:EG14325"
/db_xref="GeneID:944962"
CDS 311336..311563
/gene="ykgL"
/locus_tag="b0295"
/gene_synonym="ECK0294; JW5033"
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="NP_414829.1"
/db_xref="GI:16128280"
/db_xref="ASAP:ABE-0001019"
/db_xref="UniProtKB/Swiss-Prot:P56257"
/db_xref="EcoGene:EG14325"
/db_xref="GeneID:944962"
/translation="MQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSERNV
RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
gene complement(311598..311738)
/gene="ykgO"
/locus_tag="b4506"
/gene_synonym="ECK0295; JW5034; rpmJ2"
/db_xref="EcoGene:EG14432"
/db_xref="GeneID:1450238"
CDS complement(311598..311738)
/gene="ykgO"
/locus_tag="b4506"
/gene_synonym="ECK0295; JW5034; rpmJ2"
/codon_start=1
/transl_table=11
/product="RpmJ-like protein"
/protein_id="YP_588437.1"
/db_xref="GI:94541137"
/db_xref="ASAP:ABE-0285027"
/db_xref="EcoGene:EG14432"
/db_xref="GeneID:1450238"
/translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
KR"
misc_feature complement(311616..311738)
/gene="ykgO"
/locus_tag="b4506"
/gene_synonym="ECK0295; JW5034; rpmJ2"
/note="50S ribosomal protein L36; Validated; Region: rpmJ;
PRK00831"
/db_xref="CDD:179138"
gene complement(311738..312001)
/gene="ykgM"
/locus_tag="b0296"
/gene_synonym="ECK0296; JW5035; rpmE2"
/db_xref="EcoGene:EG14326"
/db_xref="GeneID:944960"
CDS complement(311738..312001)
/gene="ykgM"
/locus_tag="b0296"
/gene_synonym="ECK0296; JW5035; rpmE2"
/codon_start=1
/transl_table=11
/product="50S ribosomal protein L31 type B; alternative
L31 utilized during zinc limitation"
/protein_id="NP_414830.1"
/db_xref="GI:16128281"
/db_xref="ASAP:ABE-0001021"
/db_xref="UniProtKB/Swiss-Prot:P0A7N1"
/db_xref="EcoGene:EG14326"
/db_xref="GeneID:944960"
/translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
misc_feature complement(311744..312001)
/gene="ykgM"
/locus_tag="b0296"
/gene_synonym="ECK0296; JW5035; rpmE2"
/note="50S ribosomal protein L31 type B; Reviewed; Region:
rpmE2; PRK01678"
/db_xref="CDD:234969"
gene complement(312365..312466)
/gene="ykgR"
/locus_tag="b4671"
/gene_synonym="ECK4486"
/db_xref="EcoGene:EG14476"
/db_xref="GeneID:7751635"
CDS complement(312365..312466)
/gene="ykgR"
/locus_tag="b4671"
/gene_synonym="ECK4486"
/note="expressed protein"
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="YP_002791238.1"
/db_xref="GI:226524699"
/db_xref="EcoGene:EG14476"
/db_xref="GeneID:7751635"
/translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
gene complement(312940..313029)
/gene="ykgP"
/locus_tag="b4630"
/gene_synonym="ECK4446"
/pseudo
/db_xref="EcoGene:EG14433"
/db_xref="GeneID:5625556"
CDS complement(312940..313029)
/gene="ykgP"
/locus_tag="b4630"
/gene_synonym="ECK4446"
/note="pseudogene, oxidoreductase family"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="EcoGene:EG14433"
/db_xref="GeneID:5625556"
gene 313581..314452
/gene="eaeH"
/locus_tag="b0297"
/gene_synonym="ECK0297; JW0291"
/pseudo
/db_xref="EcoGene:EG12161"
/db_xref="GeneID:944957"
CDS 313581..314452
/gene="eaeH"
/locus_tag="b0297"
/gene_synonym="ECK0297; JW0291"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0009279 - cell outer membrane"
/note="pseudogene, attaching and effacing protein
homology;factor; Not classified"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="ASAP:ABE-0001025"
/db_xref="UniProtKB/Swiss-Prot:P36943"
/db_xref="EcoGene:EG12161"
/db_xref="GeneID:944957"
mobile_element 314450..315707
/mobile_element_type="insertion sequence:IS3A"
gene 314515..314814
/gene="insE1"
/locus_tag="b0298"
/gene_synonym="ECK0298"
/db_xref="EcoGene:EG40005"
/db_xref="GeneID:944952"
CDS 314515..314814
/gene="insE1"
/locus_tag="b0298"
/gene_synonym="ECK0298"
/function="IS, phage, Tn; Transposon-related functions;
extrachromosomal; transposon related"
/codon_start=1
/transl_table=11
/product="IS3 transposase A"
/protein_id="NP_414832.2"
/db_xref="GI:226524700"
/db_xref="ASAP:ABE-0001028"
/db_xref="UniProtKB/Swiss-Prot:P0CF66"
/db_xref="EcoGene:EG40005"
/db_xref="GeneID:944952"
/translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILQKAATYFAKRLK"
misc_feature 314539..314751
/gene="insE1"
/locus_tag="b0298"
/gene_synonym="ECK0298"
/note="Transposase; Region: HTH_Tnp_1; cl17663"
/db_xref="CDD:248217"
misc_feature 314542..314811
/gene="insE1"
/locus_tag="b0298"
/gene_synonym="ECK0298"
/note="Transposase and inactivated derivatives [DNA
replication, recombination, and repair]; Region: COG2963"
/db_xref="CDD:225511"
gene 314811..315677
/gene="insF1"
/locus_tag="b0299"
/gene_synonym="ECK0299"
/db_xref="EcoGene:EG40006"
/db_xref="GeneID:944948"
CDS 314811..315677
/gene="insF1"
/locus_tag="b0299"
/gene_synonym="ECK0299"
/function="IS, phage, Tn; Transposon-related functions;
extrachromosomal; transposon related"
/codon_start=1
/transl_table=11
/product="IS3 transposase B"
/protein_id="NP_414833.1"
/db_xref="GI:16128284"
/db_xref="ASAP:ABE-0001030"
/db_xref="UniProtKB/Swiss-Prot:P0CF79"
/db_xref="EcoGene:EG40006"
/db_xref="GeneID:944948"
/translation="MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQ
FRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKF
SPVSYRAHGLPVSENLLEQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVI
GWSMSPRMTAQLACDALQMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSM
SAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGG
LSPEQFENKNLA"
misc_feature 314874..315674
/gene="insF1"
/locus_tag="b0299"
/gene_synonym="ECK0299"
/note="putative transposase OrfB; Reviewed; Region:
PHA02517"
/db_xref="CDD:222853"
misc_feature 314940..315116
/gene="insF1"
/locus_tag="b0299"
/gene_synonym="ECK0299"
/note="HTH-like domain; Region: HTH_21; pfam13276"
/db_xref="CDD:222019"
misc_feature 315192..315533
/gene="insF1"
/locus_tag="b0299"
/gene_synonym="ECK0299"
/note="Integrase core domain; Region: rve; pfam00665"
/db_xref="CDD:216050"
misc_feature 315501..315671
/gene="insF1"
/locus_tag="b0299"
/gene_synonym="ECK0299"
/note="Integrase core domain; Region: rve_2; pfam13333"
/db_xref="CDD:205513"
gene complement(315710..316393)
/gene="ykgA"
/locus_tag="b0300"
/gene_synonym="ECK0300; JW5037"
/pseudo
/db_xref="EcoGene:EG13381"
/db_xref="GeneID:944956"
CDS complement(315710..316393)
/gene="ykgA"
/locus_tag="b0300"
/gene_synonym="ECK0300; JW5037"
/GO_function="GO:0016564 - transcription repressor
activity"
/GO_process="GO:0006350 - transcription"
/note="pseudogene, AraC family;putative regulator; Not
classified;
putative ARAC-type regulatory protein"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="ASAP:ABE-0001032"
/db_xref="UniProtKB/Swiss-Prot:P77601"
/db_xref="EcoGene:EG13381"
/db_xref="GeneID:944956"
gene 316663..316791
/gene="ykgQ"
/locus_tag="b4631"
/gene_synonym="ECK4454"
/pseudo
/db_xref="EcoGene:EG14443"
/db_xref="GeneID:5625557"
CDS 316663..316791
/gene="ykgQ"
/locus_tag="b4631"
/gene_synonym="ECK4454"
/note="pseudogene, putative dehydrogenase"
/pseudo
/codon_start=1
/transl_table=11
/db_xref="EcoGene:EG14443"
/db_xref="GeneID:5625557"
gene complement(316950..317543)
/gene="ykgB"
/locus_tag="b0301"
/gene_synonym="ECK0301; JW5038"
/db_xref="EcoGene:EG13579"
/db_xref="GeneID:944974"
CDS complement(316950..317543)
/gene="ykgB"
/locus_tag="b0301"
/gene_synonym="ECK0301; JW5038"
/codon_start=1
/transl_table=11
/product="inner membrane protein, DUF417 family"
/protein_id="NP_414835.2"
/db_xref="GI:90111110"
/db_xref="ASAP:ABE-0001038"
/db_xref="UniProtKB/Swiss-Prot:P75685"
/db_xref="EcoGene:EG13579"
/db_xref="GeneID:944974"
/translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
misc_feature complement(316986..317543)
/gene="ykgB"
/locus_tag="b0301"
/gene_synonym="ECK0301; JW5038"
/note="Predicted membrane protein [Function unknown];
Region: COG3059"
/db_xref="CDD:225601"
gene complement(317555..317791)
/gene="ykgI"
/locus_tag="b0303"
/gene_synonym="ECK0302; JW5039"
/db_xref="EcoGene:EG14278"
/db_xref="GeneID:945658"
CDS complement(317555..317791)
/gene="ykgI"
/locus_tag="b0303"
/gene_synonym="ECK0302; JW5039"
/note="the overlapping ORF b0302 on the opposite strand is
no longer thought to be an actual gene, and has been
deaccessioned"
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="NP_414837.2"
/db_xref="GI:90111111"
/db_xref="ASAP:ABE-0001043"
/db_xref="UniProtKB/Swiss-Prot:P75687"
/db_xref="EcoGene:EG14278"
/db_xref="GeneID:945658"
/translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
misc_feature complement(317558..317701)
/gene="ykgI"
/locus_tag="b0303"
/gene_synonym="ECK0302; JW5039"
/note="Protein of unknown function (DUF1471); Region:
DUF1471; pfam07338"
/db_xref="CDD:219380"
gene complement(317900..319225)
/gene="ykgC"
/locus_tag="b0304"
/gene_synonym="ECK0303; JW5040"
/db_xref="EcoGene:EG13580"
/db_xref="GeneID:946092"
CDS complement(317900..319225)
/gene="ykgC"
/locus_tag="b0304"
/gene_synonym="ECK0303; JW5040"
/function="putative enzyme; Not classified"
/note="putative oxidoreductase"
/codon_start=1
/transl_table=11
/product="putative pyridine nucleotide-disulfide
oxidoreductase"
/protein_id="NP_414838.2"
/db_xref="GI:90111112"
/db_xref="ASAP:ABE-0001048"
/db_xref="UniProtKB/Swiss-Prot:P77212"
/db_xref="EcoGene:EG13580"
/db_xref="GeneID:946092"
/translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRMLGA
SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
misc_feature complement(317903..319225)
/gene="ykgC"
/locus_tag="b0304"
/gene_synonym="ECK0303; JW5040"
/note="pyridine nucleotide-disulfide oxidoreductase;
Provisional; Region: PRK08010"
/db_xref="CDD:181196"
misc_feature complement(<319124..319219)
/gene="ykgC"
/locus_tag="b0304"
/gene_synonym="ECK0303; JW5040"
/note="ubiquinone biosynthesis hydroxylase family protein;
Provisional; Region: PRK07608; cl17314"
/db_xref="CDD:247868"
misc_feature complement(318521..318715)
/gene="ykgC"
/locus_tag="b0304"
/gene_synonym="ECK0303; JW5040"
/note="Pyridine nucleotide-disulphide oxidoreductase;
Region: Pyr_redox; pfam00070"
/db_xref="CDD:215691"
misc_feature complement(317927..318244)
/gene="ykgC"
/locus_tag="b0304"
/gene_synonym="ECK0303; JW5040"
/note="Pyridine nucleotide-disulphide oxidoreductase,
dimerisation domain; Region: Pyr_redox_dim; pfam02852"
/db_xref="CDD:217252"
gene 319451..320305
/gene="ykgD"
/locus_tag="b0305"
/gene_synonym="ECK0304; JW0298"
/db_xref="EcoGene:EG13581"
/db_xref="GeneID:945616"
CDS 319451..320305
/gene="ykgD"
/locus_tag="b0305"
/gene_synonym="ECK0304; JW0298"
/function="putative regulator; Not classified"
/GO_function="GO:0016563 - transcription activator
activity"
/GO_process="GO:0006350 - transcription"
/note="putative ARAC-type regulatory protein"
/codon_start=1
/transl_table=11
/product="putative DNA-binding transcriptional regulator"
/protein_id="NP_414839.1"
/db_xref="GI:16128290"
/db_xref="ASAP:ABE-0001054"
/db_xref="UniProtKB/Swiss-Prot:P77379"
/db_xref="EcoGene:EG13581"
/db_xref="GeneID:945616"
/translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
GTTPLAVLTKLRLQIAAQMFSRETLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
ERVRQLAP"
misc_feature 319454..319936
/gene="ykgD"
/locus_tag="b0305"
/gene_synonym="ECK0304; JW0298"
/note="Cupin; Region: Cupin_6; pfam12852"
/db_xref="CDD:221809"
misc_feature 319934..320299
/gene="ykgD"
/locus_tag="b0305"
/gene_synonym="ECK0304; JW0298"
/note="AraC-type DNA-binding domain-containing proteins
[Transcription]; Region: AraC; COG2207"
/db_xref="CDD:225117"
misc_feature <320192..320278
/gene="ykgD"
/locus_tag="b0305"
/gene_synonym="ECK0304; JW0298"
/note="Bacterial regulatory helix-turn-helix proteins,
AraC family; Region: HTH_AraC; pfam00165"
/db_xref="CDD:215763"
gene 320832..321551
/gene="ykgE"
/locus_tag="b0306"
/gene_synonym="ECK0305; JW5041"
/db_xref="EcoGene:EG13582"
/db_xref="GeneID:948438"
CDS 320832..321551
/gene="ykgE"
/locus_tag="b0306"
/gene_synonym="ECK0305; JW5041"
/function="putative enzyme; Not classified"
/note="putative dehydrogenase subunit"
/codon_start=1
/transl_table=11
/product="putative oxidoreductase"
/protein_id="NP_414840.1"
/db_xref="GI:16128291"
/db_xref="ASAP:ABE-0001058"
/db_xref="UniProtKB/Swiss-Prot:P77252"
/db_xref="EcoGene:EG13582"
/db_xref="GeneID:948438"
/translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAA
KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
RLQREGQKVKVMHIAEVLMSR"
misc_feature <320832..321548
/gene="ykgE"
/locus_tag="b0306"
/gene_synonym="ECK0305; JW5041"
/note="Fe-S oxidoreductase [Energy production and
conversion]; Region: GlpC; COG0247"
/db_xref="CDD:223325"
misc_feature 320838..321086
/gene="ykgE"
/locus_tag="b0306"
/gene_synonym="ECK0305; JW5041"
/note="Cysteine-rich domain; Region: CCG; pfam02754"
/db_xref="CDD:202376"
misc_feature 321225..321482
/gene="ykgE"
/locus_tag="b0306"
/gene_synonym="ECK0305; JW5041"
/note="Cysteine-rich domain; Region: CCG; pfam02754"
/db_xref="CDD:202376"
gene 321562..322989
/gene="ykgF"
/locus_tag="b0307"
/gene_synonym="ECK0306; JW0300"
/db_xref="EcoGene:EG13583"
/db_xref="GeneID:944980"
CDS 321562..322989
/gene="ykgF"
/locus_tag="b0307"
/gene_synonym="ECK0306; JW0300"
/codon_start=1
/transl_table=11
/product="putative electron transport protein with
ferridoxin-like domai"
/protein_id="NP_414841.1"
/db_xref="GI:16128292"
/db_xref="ASAP:ABE-0001060"
/db_xref="UniProtKB/Swiss-Prot:P77536"
/db_xref="EcoGene:EG13583"
/db_xref="GeneID:944980"
/translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFARTKEDATRYILQVAQ
RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
DLPEADGESFRSWFKKHQAQEKKNG"
misc_feature 321613..322920
/gene="ykgF"
/locus_tag="b0307"
/gene_synonym="ECK0306; JW0300"
/note="iron-sulfur cluster-binding protein; Region:
TIGR00273"
/db_xref="CDD:129374"
misc_feature 321775..322443
/gene="ykgF"
/locus_tag="b0307"
/gene_synonym="ECK0306; JW0300"
/note="Uncharacterized ACR, YkgG family COG1556; Region:
DUF162; pfam02589"
/db_xref="CDD:217126"
misc_feature <322474..>322740
/gene="ykgF"
/locus_tag="b0307"
/gene_synonym="ECK0306; JW0300"
/note="The HCP family of iron-sulfur proteins includes
hybrid cluster protein (HCP), acetyl-CoA synthase (ACS),
and carbon monoxide dehydrogenase (CODH), all of which
contain [Fe4-S4] metal clusters at their active sites.
These proteins have a conserved...; Region: HCP_like;
cl14655"
/db_xref="CDD:246686"
misc_feature 322714..322974
/gene="ykgF"
/locus_tag="b0307"
/gene_synonym="ECK0306; JW0300"
/note="Domain of unknown function (DUF3390); Region:
DUF3390; pfam11870"
/db_xref="CDD:221280"
gene 322982..323677
/gene="ykgG"
/locus_tag="b0308"
/gene_synonym="ECK0307; JW5042"
/db_xref="EcoGene:EG13584"
/db_xref="GeneID:945906"
CDS 322982..323677
/gene="ykgG"
/locus_tag="b0308"
/gene_synonym="ECK0307; JW5042"
/function="putative transport; Not classified"
/codon_start=1
/transl_table=11
/product="putative transporter"
/protein_id="NP_414842.4"
/db_xref="GI:90111113"
/db_xref="ASAP:ABE-0001062"
/db_xref="UniProtKB/Swiss-Prot:P77433"
/db_xref="EcoGene:EG13584"
/db_xref="GeneID:945906"
/translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
EYSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
GPVKAVYLIIEDC"
misc_feature 322982..323674
/gene="ykgG"
/locus_tag="b0308"
/gene_synonym="ECK0307; JW5042"
/note="Uncharacterized conserved protein [Function
unknown]; Region: COG1556"
/db_xref="CDD:224473"
gene complement(323920..324588)
/gene="ykgH"
/locus_tag="b0310"
/gene_synonym="ECK0308; JW0302"
/db_xref="EcoGene:EG13585"
/db_xref="GeneID:947513"
CDS complement(323920..324588)
/gene="ykgH"
/locus_tag="b0310"
/gene_synonym="ECK0308; JW0302"
/codon_start=1
/transl_table=11
/product="putative inner membrane protein"
/protein_id="NP_414844.1"
/db_xref="GI:16128295"
/db_xref="ASAP:ABE-0001066"
/db_xref="UniProtKB/Swiss-Prot:P77180"
/db_xref="EcoGene:EG13585"
/db_xref="GeneID:947513"
/translation="MREQIKQDIDLIEILFYLKKKIRVILFIMAICMAMVLLFLYINK
DNIKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIR
LEWSGDKRALPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
NSGQ"
misc_feature complement(323953..324186)
/gene="ykgH"
/locus_tag="b0310"
/gene_synonym="ECK0308; JW0302"
/note="G-rich domain on putative tyrosine kinase; Region:
GNVR; pfam13807"
/db_xref="CDD:222392"
gene complement(324801..326471)
/gene="betA"
/locus_tag="b0311"
/gene_synonym="ECK0309; JW0303"
/db_xref="EcoGene:EG10109"
/db_xref="GeneID:945716"
CDS complement(324801..326471)
/gene="betA"
/locus_tag="b0311"
/gene_synonym="ECK0309; JW0303"
/EC_number="1.1.99.1"
/function="enzyme; Osmotic adaptation"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/GO_process="GO:0006578 - betaine biosynthetic process"
/GO_process="GO:0006970 - response to osmotic stress"
/codon_start=1
/transl_table=11
/product="choline dehydrogenase, a flavoprotein"
/protein_id="NP_414845.1"
/db_xref="GI:16128296"
/db_xref="ASAP:ABE-0001070"
/db_xref="UniProtKB/Swiss-Prot:P17444"
/db_xref="EcoGene:EG10109"
/db_xref="GeneID:945716"
/translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
DLDNWAQEPGLENWSYLDCLPYYRKAETRDMGENDYHGGDGPVSVTTSKPGVNPLFEA
MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
misc_feature complement(324807..326471)
/gene="betA"
/locus_tag="b0311"
/gene_synonym="ECK0309; JW0303"
/note="choline dehydrogenase; Validated; Region: PRK02106"
/db_xref="CDD:235000"
misc_feature complement(324891..325295)
/gene="betA"
/locus_tag="b0311"
/gene_synonym="ECK0309; JW0303"
/note="GMC oxidoreductase; Region: GMC_oxred_C; pfam05199"
/db_xref="CDD:218493"
gene complement(326485..327957)
/gene="betB"
/locus_tag="b0312"
/gene_synonym="ECK0310; JW0304"
/db_xref="EcoGene:EG10110"
/db_xref="GeneID:947376"
CDS complement(326485..327957)
/gene="betB"
/locus_tag="b0312"
/gene_synonym="ECK0310; JW0304"
/EC_number="1.2.1.8"
/function="enzyme; Osmotic adaptation"
/GO_process="GO:0006578 - betaine biosynthetic process"
/GO_process="GO:0006970 - response to osmotic stress"
/note="NAD+-dependent betaine aldehyde dehydrogenase"
/codon_start=1
/transl_table=11
/product="betaine aldehyde dehydrogenase, NAD-dependent"
/protein_id="NP_414846.1"
/db_xref="GI:16128297"
/db_xref="ASAP:ABE-0001072"
/db_xref="UniProtKB/Swiss-Prot:P17445"
/db_xref="EcoGene:EG10110"
/db_xref="GeneID:947376"
/translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
DVDRAVKSAQQGQKIWASMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
misc_feature complement(326488..327957)
/gene="betB"
/locus_tag="b0312"
/gene_synonym="ECK0310; JW0304"
/note="betaine aldehyde dehydrogenase; Provisional;
Region: PRK13252"
/db_xref="CDD:183918"
mat_peptide complement(326488..327954)
/gene="betB"
/locus_tag="b0312"
/gene_synonym="ECK0310; JW0304"
/product="betaine aldehyde dehydrogenase, NAD-dependent"
/experiment="N-terminus verified by Edman degradation:
PMID 1956285"
misc_feature complement(326509..327882)
/gene="betB"
/locus_tag="b0312"
/gene_synonym="ECK0310; JW0304"
/note="NAD+-dependent 4-trimethylaminobutyraldehyde
dehydrogenase, ALDH family 9A1; Region: ALDH_F9_TMBADH;
cd07090"
/db_xref="CDD:143409"
misc_feature complement(order(326509..326514,326518..326535,
326569..326571,326575..326577,326584..326586,
326590..326592,326602..326604,326608..326610,
326632..326637,326641..326652,326656..326658,
326665..326667,326671..326676,326683..326694,
326986..326988,327031..327033,327130..327135,
327142..327144,327151..327153,327217..327219,
327253..327255,327535..327543,327547..327549,
327571..327585,327604..327606,327616..327618,
327718..327720,327727..327729,327739..327741,
327748..327750,327760..327762,327766..327768))
/gene="betB"
/locus_tag="b0312"
/gene_synonym="ECK0310; JW0304"
/note="tetrameric interface [polypeptide binding]; other
site"
/db_xref="CDD:143409"
misc_feature complement(order(326599..326601,326791..326793,
326797..326799,327100..327102,327196..327204,
327259..327261,327268..327273,327277..327279,
327331..327333,327430..327432,327499..327504,
327508..327513))
/gene="betB"
/locus_tag="b0312"
/gene_synonym="ECK0310; JW0304"
/note="NAD binding site [chemical binding]; other site"
/db_xref="CDD:143409"
misc_feature complement(order(327100..327102,327109..327111,
327202..327204,327499..327501))
/gene="betB"
/locus_tag="b0312"
/gene_synonym="ECK0310; JW0304"
/note="catalytic residues [active]"
/db_xref="CDD:143409"
gene complement(327971..328558)
/gene="betI"
/locus_tag="b0313"
/gene_synonym="ECK0311; JW0305"
/db_xref="EcoGene:EG10111"
/db_xref="GeneID:944981"
CDS complement(327971..328558)
/gene="betI"
/locus_tag="b0313"
/gene_synonym="ECK0311; JW0305"
/function="putative regulator; Osmotic adaptation"
/GO_function="GO:0016564 - transcription repressor
activity"
/GO_process="GO:0006350 - transcription"
/GO_process="GO:0006970 - response to osmotic stress"
/note="probably transcriptional repressor of bet genes"
/codon_start=1
/transl_table=11
/product="DNA-binding transcriptional repressor"
/protein_id="NP_414847.3"
/db_xref="GI:49176011"
/db_xref="ASAP:ABE-0001076"
/db_xref="UniProtKB/Swiss-Prot:P17446"
/db_xref="EcoGene:EG10111"
/db_xref="GeneID:944981"
/translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
IDGLWLRAALSGKPLDKTRANSLTRHFITQHLPTD"
misc_feature complement(327977..328558)
/gene="betI"
/locus_tag="b0313"
/gene_synonym="ECK0311; JW0305"
/note="transcriptional regulator BetI; Validated; Region:
PRK00767"
/db_xref="CDD:179115"
misc_feature complement(328382..328519)
/gene="betI"
/locus_tag="b0313"
/gene_synonym="ECK0311; JW0305"
/note="Bacterial regulatory proteins, tetR family; Region:
TetR_N; pfam00440"
/db_xref="CDD:201228"
misc_feature complement(327983..328315)
/gene="betI"
/locus_tag="b0313"
/gene_synonym="ECK0311; JW0305"
/note="Bacterial transcriptional repressor; Region:
TetR_C_6; pfam13977"
/db_xref="CDD:222485"
gene 328687..330720
/gene="betT"
/locus_tag="b0314"
/gene_synonym="ECK0312; JW0306"
/db_xref="EcoGene:EG10112"
/db_xref="GeneID:945079"
CDS 328687..330720
/gene="betT"
/locus_tag="b0314"
/gene_synonym="ECK0312; JW0306"
/function="transport; Transport of small molecules: Other"
/GO_component="GO:0009274 - peptidoglycan-based cell wall"
/GO_component="GO:0019866 - organelle inner membrane"
/GO_process="GO:0006578 - betaine biosynthetic process"
/note="high-affinity choline transport"
/codon_start=1
/transl_table=11
/product="choline transporter of high affinity"
/protein_id="NP_414848.1"
/db_xref="GI:16128299"
/db_xref="ASAP:ABE-0001080"
/db_xref="UniProtKB/Swiss-Prot:P0ABC9"
/db_xref="EcoGene:EG10112"
/db_xref="GeneID:945079"
/translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
misc_feature 328687..330717
/gene="betT"
/locus_tag="b0314"
/gene_synonym="ECK0312; JW0306"
/note="choline transport protein BetT; Provisional;
Region: PRK09928"
/db_xref="CDD:236635"
gene 331595..332683
/gene="yahA"
/locus_tag="b0315"
/gene_synonym="ECK0313; JW0307"
/db_xref="EcoGene:EG11236"
/db_xref="GeneID:947459"
CDS 331595..332683
/gene="yahA"
/locus_tag="b0315"
/gene_synonym="ECK0313; JW0307"
/GO_process="GO:0006355 - regulation of transcription,
DNA-dependent"
/codon_start=1
/transl_table=11
/product="c-di-GMP-specific phosphodiesterase"
/protein_id="NP_414849.1"
/db_xref="GI:16128300"
/db_xref="ASAP:ABE-0001086"
/db_xref="UniProtKB/Swiss-Prot:P21514"
/db_xref="EcoGene:EG11236"
/db_xref="GeneID:947459"
/translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGSNSHRYINDN
HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
VNKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
VHFLQGYLYSPPVPGNKFISEWVMKAGG"
misc_feature 331670..331834
/gene="yahA"
/locus_tag="b0315"
/gene_synonym="ECK0313; JW0307"
/note="C-terminal DNA-binding domain of LuxR-like
proteins. This domain contains a helix-turn-helix motif
and binds DNA. Proteins belonging to this group are
response regulators; some act as transcriptional
activators, others as transcriptional repressors. Many...;
Region: LuxR_C_like; cd06170"
/db_xref="CDD:99777"
misc_feature order(331670..331678,331715..331723,331745..331750,
331754..331759,331763..331777,331808..331810)
/gene="yahA"
/locus_tag="b0315"
/gene_synonym="ECK0313; JW0307"
/note="DNA binding residues [nucleotide binding]"
/db_xref="CDD:99777"
misc_feature order(331703..331705,331709..331711,331715..331717,
331808..331816,331823..331825,331832..331834)
/gene="yahA"
/locus_tag="b0315"
/gene_synonym="ECK0313; JW0307"
/note="dimerization interface [polypeptide binding]; other
site"
/db_xref="CDD:99777"
misc_feature 331925..332638
/gene="yahA"
/locus_tag="b0315"
/gene_synonym="ECK0313; JW0307"
/note="EAL domain. This domain is found in diverse
bacterial signaling proteins. It is called EAL after its
conserved residues and is also known as domain of unknown
function 2 (DUF2). The EAL domain has been shown to
stimulate degradation of a second...; Region: EAL;
cd01948"
/db_xref="CDD:238923"
gene complement(332725..333657)
/gene="yahB"
/locus_tag="b0316"
/gene_synonym="ECK0314; JW0308"
/db_xref="EcoGene:EG13586"
/db_xref="GeneID:945278"
CDS complement(332725..333657)
/gene="yahB"
/locus_tag="b0316"
/gene_synonym="ECK0314; JW0308"
/function="putative regulator; Not classified"
/GO_function="GO:0016563 - transcription activator
activity"
/GO_function="GO:0016564 - transcription repressor
activity"
/GO_process="GO:0006350 - transcription"
/note="putative transcriptional regulator LYSR-type"
/codon_start=1
/transl_table=11
/product="putative DNA-bindng transcriptional regulator"
/protein_id="NP_414850.1"
/db_xref="GI:16128301"
/db_xref="ASAP:ABE-0001088"
/db_xref="UniProtKB/Swiss-Prot:P77700"
/db_xref="EcoGene:EG13586"
/db_xref="GeneID:945278"
/translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRRIDTISQGIEARVRICIN
QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
misc_feature complement(332779..333645)
/gene="yahB"
/locus_tag="b0316"
/gene_synonym="ECK0314; JW0308"
/note="DNA-binding transcriptional activator AllS;
Provisional; Region: PRK10094"
/db_xref="CDD:182237"
misc_feature complement(333460..333630)
/gene="yahB"
/locus_tag="b0316"
/gene_synonym="ECK0314; JW0308"
/note="Bacterial regulatory helix-turn-helix protein, lysR
family; Region: HTH_1; pfam00126"
/db_xref="CDD:215735"
misc_feature complement(332797..333366)
/gene="yahB"
/locus_tag="b0316"
/gene_synonym="ECK0314; JW0308"
/note="The substrate binding domain of LysR-type
transcriptional regulators (LTTRs), a member of the type 2
periplasmic binding fold protein superfamily; Region:
PBP2_LTTR_substrate; cl11398"
/db_xref="CDD:245600"
misc_feature complement(order(332947..332952,332956..332961,
332977..332994,333265..333285,333289..333291,
333301..333306,333310..333315))
/gene="yahB"
/locus_tag="b0316"
/gene_synonym="ECK0314; JW0308"
/note="dimerization interface [polypeptide binding]; other
site"
/db_xref="CDD:176102"
gene complement(333749..334246)
/gene="yahC"
/locus_tag="b0317"
/gene_synonym="ECK0315; JW0309"
/db_xref="EcoGene:EG13587"
/db_xref="GeneID:947337"
CDS complement(333749..334246)
/gene="yahC"
/locus_tag="b0317"
/gene_synonym="ECK0315; JW0309"
/codon_start=1
/transl_table=11
/product="putative inner membrane protein"
/protein_id="NP_414851.1"
/db_xref="GI:16128302"
/db_xref="ASAP:ABE-0001091"
/db_xref="UniProtKB/Swiss-Prot:P77219"
/db_xref="EcoGene:EG13587"
/db_xref="GeneID:947337"
/translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGVISEYGGQKLSEATT
KRDGY"
misc_feature complement(333776..334222)
/gene="yahC"
/locus_tag="b0317"
/gene_synonym="ECK0315; JW0309"
/note="Protein of unknown function (DUF1097); Region:
DUF1097; pfam06496"
/db_xref="CDD:219062"
gene 334504..335109
/gene="yahD"
/locus_tag="b0318"
/gene_synonym="ECK0316; JW0310"
/db_xref="EcoGene:EG13588"
/db_xref="GeneID:947681"
CDS 334504..335109
/gene="yahD"
/locus_tag="b0318"
/gene_synonym="ECK0316; JW0310"
/function="putative regulator; Not classified"
/note="putative transcription factor"
/codon_start=1
/transl_table=11
/product="ankyrin repeat protein"
/protein_id="NP_414852.1"
/db_xref="GI:16128303"
/db_xref="ASAP:ABE-0001095"
/db_xref="UniProtKB/Swiss-Prot:P77736"
/db_xref="EcoGene:EG13588"
/db_xref="GeneID:947681"
/translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
misc_feature 334537..334800
/gene="yahD"
/locus_tag="b0318"
/gene_synonym="ECK0316; JW0310"
/note="Ankyrin repeats (3 copies); Region: Ank_2;
pfam12796"
/db_xref="CDD:205076"
misc_feature 334600..335094
/gene="yahD"
/locus_tag="b0318"
/gene_synonym="ECK0316; JW0310"
/note="ankyrin repeats; ankyrin repeats mediate
protein-protein interactions in very diverse families of
proteins. The number of ANK repeats in a protein can range
from 2 to over 20 (ankyrins, for example). ANK repeats may
occur in combinations with other...; Region: ANK; cd00204"
/db_xref="CDD:238125"
misc_feature 334729..335025
/gene="yahD"
/locus_tag="b0318"
/gene_synonym="ECK0316; JW0310"
/note="Ankyrin repeats (3 copies); Region: Ank_2;
pfam12796"
/db_xref="CDD:205076"
gene 335149..336012
/gene="yahE"
/locus_tag="b0319"
/gene_synonym="ECK0317; JW0311"
/db_xref="EcoGene:EG13589"
/db_xref="GeneID:947134"
CDS 335149..336012
/gene="yahE"
/locus_tag="b0319"
/gene_synonym="ECK0317; JW0311"
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="NP_414853.1"
/db_xref="GI:16128304"
/db_xref="ASAP:ABE-0001097"
/db_xref="UniProtKB/Swiss-Prot:P77297"
/db_xref="EcoGene:EG13589"
/db_xref="GeneID:947134"
/translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNDKQNI
ASAVASMMGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYLGLQRGKNNTTLLS
AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
LSQTYGGNYVS"
misc_feature 335647..335979
/gene="yahE"
/locus_tag="b0319"
/gene_synonym="ECK0317; JW0311"
/note="Protein of unknown function (DUF2877); Region:
DUF2877; pfam11392"
/db_xref="CDD:221103"
gene 336002..337549
/gene="yahF"
/locus_tag="b0320"
/gene_synonym="ECK0318; JW0312"
/db_xref="EcoGene:EG13590"
/db_xref="GeneID:947298"
CDS 336002..337549
/gene="yahF"
/locus_tag="b0320"
/gene_synonym="ECK0318; JW0312"
/function="putative enzyme; Not classified"
/note="putative oxidoreductase subunit"
/codon_start=1
/transl_table=11
/product="putative acyl-CoA synthetase with NAD(P)-binding
domain and succinyl-CoA synthetase domain"
/protein_id="NP_414854.1"
/db_xref="GI:16128305"
/db_xref="ASAP:ABE-0001099"
/db_xref="UniProtKB/Swiss-Prot:P77187"
/db_xref="EcoGene:EG13590"
/db_xref="GeneID:947298"
/translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
TIGSAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
PVVVCFLDRGETPVDEQGLQFARGTKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLKDINRSIKHTFLD
FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIETI
KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
ICKGEEA"
misc_feature 336056..337117
/gene="yahF"
/locus_tag="b0320"
/gene_synonym="ECK0318; JW0312"
/note="Succinyl-CoA synthetase, alpha subunit [Energy
production and conversion]; Region: SucD; COG0074"
/db_xref="CDD:223152"
misc_feature 336560..336850
/gene="yahF"
/locus_tag="b0320"
/gene_synonym="ECK0318; JW0312"
/note="CoA binding domain; Region: CoA_binding; pfam02629"
/db_xref="CDD:202320"
misc_feature 337037..337516
/gene="yahF"
/locus_tag="b0320"
/gene_synonym="ECK0318; JW0312"
/note="CoA-ligase; Region: Ligase_CoA; pfam00549"
/db_xref="CDD:215988"
gene 337549..338967
/gene="yahG"
/locus_tag="b0321"
/gene_synonym="ECK0319; JW0313"
/db_xref="EcoGene:EG13591"
/db_xref="GeneID:945379"
CDS 337549..338967
/gene="yahG"
/locus_tag="b0321"
/gene_synonym="ECK0319; JW0313"
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="NP_414855.1"
/db_xref="GI:16128306"
/db_xref="ASAP:ABE-0001101"
/db_xref="UniProtKB/Swiss-Prot:P77221"
/db_xref="EcoGene:EG13591"
/db_xref="GeneID:945379"
/translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTAKTILHAG
PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
IVHPPFACFEKAILGWCERYGV"
misc_feature 337975..338622
/gene="yahG"
/locus_tag="b0321"
/gene_synonym="ECK0319; JW0313"
/note="Protein of unknown function (DUF1116); Region:
DUF1116; pfam06545"
/db_xref="CDD:148259"
repeat_region 338981..339338
/note="REP23 (repetitive extragenic palindromic) element;
contains 8 REP sequences"
gene 339389..340339
/gene="yahI"
/locus_tag="b0323"
/gene_synonym="ECK0321; JW0315"
/db_xref="EcoGene:EG13593"
/db_xref="GeneID:944984"
CDS 339389..340339
/gene="yahI"
/locus_tag="b0323"
/gene_synonym="ECK0321; JW0315"
/function="putative enzyme; Not classified"
/GO_process="GO:0006526 - arginine biosynthetic process"
/GO_process="GO:0006221 - pyrimidine nucleotide
biosynthetic process"
/note="putative kinase"
/codon_start=1
/transl_table=11
/product="carbamate kinase-like protein"
/protein_id="NP_414857.1"
/db_xref="GI:16128308"
/db_xref="ASAP:ABE-0001107"
/db_xref="UniProtKB/Swiss-Prot:P77624"
/db_xref="EcoGene:EG13593"
/db_xref="GeneID:944984"
/translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
YDIVLTHGNGPQVGLDLRRAEIAHKREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSDSQRDELQKANPDWCFVEDAGRG
YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
misc_feature 339389..340333
/gene="yahI"
/locus_tag="b0323"
/gene_synonym="ECK0321; JW0315"
/note="carbamate kinase-like carbamoyl phosphate
synthetase; Reviewed; Region: PRK12454"
/db_xref="CDD:183535"
misc_feature 339398..340330
/gene="yahI"
/locus_tag="b0323"
/gene_synonym="ECK0321; JW0315"
/note="AAK_CK: Carbamate kinase (CK) catalyzes both the
ATP-phosphorylation of carbamate and carbamoyl phosphate
(CP) utilization with the production of ATP from ADP and
CP. Both CK (this CD) and nonhomologous CP synthetase
synthesize carbamoyl phosphate, an...; Region: AAK_CK;
cd04235"
/db_xref="CDD:239768"
misc_feature order(339410..339412,339416..339421,339542..339550,
339776..339778,340031..340039)
/gene="yahI"
/locus_tag="b0323"
/gene_synonym="ECK0321; JW0315"
/note="putative substrate binding site [chemical binding];
other site"
/db_xref="CDD:239768"
misc_feature order(339419..339421,340094..340099,340112..340114,
340121..340123,340196..340198,340208..340213,
340220..340222)
/gene="yahI"
/locus_tag="b0323"
/gene_synonym="ECK0321; JW0315"
/note="nucleotide binding site [chemical binding]; other
site"
/db_xref="CDD:239768"
misc_feature order(339419..339421,340094..340099,340112..340114,
340121..340123,340196..340198,340208..340213,
340220..340222)
/gene="yahI"
/locus_tag="b0323"
/gene_synonym="ECK0321; JW0315"
/note="nucleotide binding site [chemical binding]; other
site"
/db_xref="CDD:239768"
misc_feature order(339572..339574,339614..339616,339623..339628,
339635..339637,339647..339652,339659..339661,
339668..339673,339683..339685,339719..339727,
339731..339733,339911..339913,339920..339922,
340007..340009,340013..340015)
/gene="yahI"
/locus_tag="b0323"
/gene_synonym="ECK0321; JW0315"
/note="homodimer interface [polypeptide binding]; other
site"
/db_xref="CDD:239768"
gene 340349..341731
/gene="yahJ"
/locus_tag="b0324"
/gene_synonym="ECK0322; JW0316"
/db_xref="EcoGene:EG13594"
/db_xref="GeneID:944976"
CDS 340349..341731
/gene="yahJ"
/locus_tag="b0324"
/gene_synonym="ECK0322; JW0316"
/function="putative enzyme; Not classified"
/GO_process="GO:0009310 - amine catabolic process"
/note="putative deaminase"
/codon_start=1
/transl_table=11
/product="putative deaminase with metallo-dependent
hydrolase domain"
/protein_id="NP_414858.1"
/db_xref="GI:16128309"
/db_xref="ASAP:ABE-0001109"
/db_xref="UniProtKB/Swiss-Prot:P77554"
/db_xref="EcoGene:EG13594"
/db_xref="GeneID:944976"
/translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKLHPDAT
LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELANRMVVQQISI
ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
GQLVWGSVAG"
misc_feature 340493..341728
/gene="yahJ"
/locus_tag="b0324"
/gene_synonym="ECK0322; JW0316"
/note="putative deaminase; Validated; Region: PRK06846"
/db_xref="CDD:235873"
misc_feature 340511..341710
/gene="yahJ"
/locus_tag="b0324"
/gene_synonym="ECK0322; JW0316"
/note="Bacterial cytosine deaminase and related
metal-dependent hydrolases. Cytosine deaminases (CDs)
catalyze the deamination of cytosine, producing uracil and
ammonia. They play an important role in pyrimidine
salvage. CDs are present in prokaryotes and fungi; Region:
Bact_CD; cd01293"
/db_xref="CDD:238618"
misc_feature order(340709..340711,340715..340717,341171..341173,
341180..341182,341270..341272,341432..341434)
/gene="yahJ"
/locus_tag="b0324"
/gene_synonym="ECK0322; JW0316"
/note="active site"
/db_xref="CDD:238618"
gene 342108..343157
/gene="yahK"
/locus_tag="b0325"
/gene_synonym="ECK0323; JW0317"
/db_xref="EcoGene:EG13595"
/db_xref="GeneID:944975"
CDS 342108..343157
/gene="yahK"
/locus_tag="b0325"
/gene_synonym="ECK0323; JW0317"
/function="putative enzyme; Not classified"
/note="putative oxidoreductase"
/codon_start=1
/transl_table=11
/product="putative oxidoreductase, Zn-dependent and
NAD(P)-binding"
/protein_id="NP_414859.1"
/db_xref="GI:16128310"
/db_xref="ASAP:ABE-0001113"
/db_xref="UniProtKB/Swiss-Prot:P75691"
/db_xref="EcoGene:EG13595"
/db_xref="GeneID:944975"
/translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
DVKYRFVIDNRTLTD"
misc_feature 342108..343145
/gene="yahK"
/locus_tag="b0325"
/gene_synonym="ECK0323; JW0317"
/note="Zn-dependent alcohol dehydrogenases [General
function prediction only]; Region: AdhP; COG1064"
/db_xref="CDD:223992"
misc_feature 342117..343136
/gene="yahK"
/locus_tag="b0325"
/gene_synonym="ECK0323; JW0317"
/note="Cinnamyl alcohol dehydrogenases (CAD); Region:
CAD1; cd05283"
/db_xref="CDD:176186"
misc_feature order(342225..342233,342240..342242,342579..342581,
342591..342593,342651..342668,342720..342725,
342735..342737,342780..342782,342837..342842,
342846..342848,342906..342911,342981..342989)
/gene="yahK"
/locus_tag="b0325"
/gene_synonym="ECK0323; JW0317"
/note="putative NAD(P) binding site [chemical binding];
other site"
/db_xref="CDD:176186"
misc_feature order(342225..342227,342231..342233,342291..342293,
342369..342371,342579..342581,342987..342989)
/gene="yahK"
/locus_tag="b0325"
/gene_synonym="ECK0323; JW0317"
/note="putative substrate binding site [chemical binding];
other site"
/db_xref="CDD:176186"
misc_feature order(342225..342227,342291..342293,342579..342581)
/gene="yahK"
/locus_tag="b0325"
/gene_synonym="ECK0323; JW0317"
/note="catalytic Zn binding site [ion binding]; other
site"
/db_xref="CDD:176186"
misc_feature order(342384..342386,342393..342395,342402..342404,
342426..342428)
/gene="yahK"
/locus_tag="b0325"
/gene_synonym="ECK0323; JW0317"
/note="structural Zn binding site [ion binding]; other
site"
/db_xref="CDD:176186"
misc_feature order(342423..342425,342600..342602,342612..342614,
342888..342890,342903..342911,342915..342917,
342945..342947,342951..342956,342963..342986)
/gene="yahK"
/locus_tag="b0325"
/gene_synonym="ECK0323; JW0317"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:176186"
gene 343400..344215
/gene="yahL"
/locus_tag="b0326"
/gene_synonym="ECK0324; JW0318"
/db_xref="EcoGene:EG13596"
/db_xref="GeneID:944970"
CDS 343400..344215
/gene="yahL"
/locus_tag="b0326"
/gene_synonym="ECK0324; JW0318"
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="NP_414860.1"
/db_xref="GI:16128311"
/db_xref="ASAP:ABE-0001117"
/db_xref="UniProtKB/Swiss-Prot:P77393"
/db_xref="EcoGene:EG13596"
/db_xref="GeneID:944970"
/translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
YPDHPVKFVFHILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
KCILLEYHMPLLRIFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
gene 344628..344873
/gene="yahM"
/locus_tag="b0327"
/gene_synonym="ECK0325; JW5044"
/db_xref="EcoGene:EG13597"
/db_xref="GeneID:944969"
CDS 344628..344873
/gene="yahM"
/locus_tag="b0327"
/gene_synonym="ECK0325; JW5044"
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="NP_414861.2"
/db_xref="GI:90111114"
/db_xref="ASAP:ABE-0001120"
/db_xref="UniProtKB/Swiss-Prot:P75692"
/db_xref="EcoGene:EG13597"
/db_xref="GeneID:944969"
/translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGWGRPMKKAHTGES
GLKWEAKDSSKLIGNKDHALRGLSSPMAVIRQIRLIT"
gene complement(344890..345561)
/gene="yahN"
/locus_tag="b0328"
/gene_synonym="ECK0326; JW0320"
/db_xref="EcoGene:EG13598"
/db_xref="GeneID:944968"
CDS complement(344890..345561)
/gene="yahN"
/locus_tag="b0328"
/gene_synonym="ECK0326; JW0320"
/function="putative enzyme; Not classified"
/note="putative cytochrome subunit of dehydrogenase"
/codon_start=1
/transl_table=11
/product="amino acid exporter for proline, lysine,
glutamate, homoserine"
/protein_id="NP_414862.1"
/db_xref="GI:16128313"
/db_xref="ASAP:ABE-0001122"
/db_xref="UniProtKB/Swiss-Prot:P75693"
/db_xref="EcoGene:EG13598"
/db_xref="GeneID:944968"
/translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
GVTQR"
misc_feature complement(344917..345465)
/gene="yahN"
/locus_tag="b0328"
/gene_synonym="ECK0326; JW0320"
/note="The Resistance to Homoserine/Threonine (RhtB)
Family protein; Region: 2A76; TIGR00949"
/db_xref="CDD:233204"
STS 345679..346310
/standard_name="D14S163"
/db_xref="UniSTS:148387"
gene 345708..345983
/gene="yahO"
/locus_tag="b0329"
/gene_synonym="ECK0327; JW0321"
/db_xref="EcoGene:EG13599"
/db_xref="GeneID:944985"
CDS 345708..345983
/gene="yahO"
/locus_tag="b0329"
/gene_synonym="ECK0327; JW0321"
/codon_start=1
/transl_table=11
/product="periplasmic protein, function unknown, YhcN
family"
/protein_id="NP_414863.1"
/db_xref="GI:16128314"
/db_xref="ASAP:ABE-0001130"
/db_xref="UniProtKB/Swiss-Prot:P75694"
/db_xref="EcoGene:EG13599"
/db_xref="GeneID:944985"
/translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
misc_feature 345708..345980
/gene="yahO"
/locus_tag="b0329"
/gene_synonym="ECK0327; JW0321"
/note="hypothetical protein; Provisional; Region:
PRK09929"
/db_xref="CDD:182151"
repeat_region 346048..346077
/note="REP24 (repetitive extragenic palindromic) element;
contains 1 REP sequences"
gene complement(346081..347667)
/gene="prpR"
/locus_tag="b0330"
/gene_synonym="ECK0328; JW0322; yahP"
/db_xref="EcoGene:EG13600"
/db_xref="GeneID:944987"
CDS complement(346081..347667)
/gene="prpR"
/locus_tag="b0330"
/gene_synonym="ECK0328; JW0322; yahP"
/function="regulator; Not classified"
/GO_component="GO:0005737 - cytoplasm"
/GO_function="GO:0016564 - transcription repressor
activity"
/GO_process="GO:0019395 - fatty acid oxidation"
/GO_process="GO:0006350 - transcription"
/note="regulator for prp operon"
/codon_start=1
/transl_table=11
/product="propionate catabolism operon regulatory protein"
/protein_id="NP_414864.1"
/db_xref="GI:16128315"
/db_xref="ASAP:ABE-0001133"
/db_xref="UniProtKB/Swiss-Prot:P77743"
/db_xref="EcoGene:EG13600"
/db_xref="GeneID:944987"
/translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGRFRRDLFYRLSILR
LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLLHYDWPGNIRE
LRNMMERLALFLSVEPTPDLTPQFMQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
TAAANYLGISRTTFWRRLKS"
misc_feature complement(346084..347667)
/gene="prpR"
/locus_tag="b0330"
/gene_synonym="ECK0328; JW0322; yahP"
/note="propionate catabolism operon regulatory protein
PrpR; Provisional; Region: PRK15424"
/db_xref="CDD:237963"
misc_feature complement(347083..347586)
/gene="prpR"
/locus_tag="b0330"
/gene_synonym="ECK0328; JW0322; yahP"
/note="Propionate catabolism activator; Region: PrpR_N;
pfam06506"
/db_xref="CDD:219065"
misc_feature complement(346477..347010)
/gene="prpR"
/locus_tag="b0330"
/gene_synonym="ECK0328; JW0322; yahP"
/note="The AAA+ (ATPases Associated with a wide variety of
cellular Activities) superfamily represents an ancient
group of ATPases belonging to the ASCE (for additional
strand, catalytic E) division of the P-loop NTPase fold.
The ASCE division also includes ABC; Region: AAA; cd00009"
/db_xref="CDD:99707"
misc_feature complement(346909..346932)
/gene="prpR"
/locus_tag="b0330"
/gene_synonym="ECK0328; JW0322; yahP"
/note="Walker A motif; other site"
/db_xref="CDD:99707"
misc_feature complement(order(346564..346566,346690..346692,
346906..346929))
/gene="prpR"
/locus_tag="b0330"
/gene_synonym="ECK0328; JW0322; yahP"
/note="ATP binding site [chemical binding]; other site"
/db_xref="CDD:99707"
misc_feature complement(346687..346704)
/gene="prpR"
/locus_tag="b0330"
/gene_synonym="ECK0328; JW0322; yahP"
/note="Walker B motif; other site"
/db_xref="CDD:99707"
misc_feature complement(346507..346509)
/gene="prpR"
/locus_tag="b0330"
/gene_synonym="ECK0328; JW0322; yahP"
/note="arginine finger; other site"
/db_xref="CDD:99707"
misc_feature complement(346087..>346176)
/gene="prpR"
/locus_tag="b0330"
/gene_synonym="ECK0328; JW0322; yahP"
/note="Bacterial regulatory protein, Fis family; Region:
HTH_8; pfam02954"
/db_xref="CDD:202485"
gene 347906..348796
/gene="prpB"
/locus_tag="b0331"
/gene_synonym="ECK0329; JW0323; yahQ"
/db_xref="EcoGene:EG13601"
/db_xref="GeneID:944990"
CDS 347906..348796
/gene="prpB"
/locus_tag="b0331"
/gene_synonym="ECK0329; JW0323; yahQ"
/EC_number="4.1.3.30"
/function="putative enzyme; Not classified"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0019395 - fatty acid oxidation"
/note="putative phosphonomutase 2"
/codon_start=1
/transl_table=11
/product="2-methylisocitrate lyase"
/protein_id="NP_414865.1"
/db_xref="GI:16128316"
/db_xref="ASAP:ABE-0001138"
/db_xref="UniProtKB/Swiss-Prot:P77541"
/db_xref="EcoGene:EG13601"
/db_xref="GeneID:944990"
/translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
INYYQYEEKLDNLFARSQVK"
misc_feature 347933..348658
/gene="prpB"
/locus_tag="b0331"
/gene_synonym="ECK0329; JW0323; yahQ"
/note="Members of the ICL/PEPM enzyme family catalyze
either P-C or C-C bond formation/cleavage. Known members
are phosphoenolpyruvate mutase (PEPM), phosphonopyruvate
hydrolase (PPH), carboxyPEP mutase (CPEP mutase),
oxaloacetate hydrolase (OAH), isocitrate...; Region:
ICL_PEPM; cd00377"
/db_xref="CDD:119340"
misc_feature 347933..348655
/gene="prpB"
/locus_tag="b0331"
/gene_synonym="ECK0329; JW0323; yahQ"
/note="Phosphoenolpyruvate phosphomutase; Region:
PEP_mutase; pfam13714"
/db_xref="CDD:222337"
misc_feature order(347939..347941,347960..347962,347966..347977,
347981..347995,347999..348004,348011..348013,
348017..348025,348044..348046,348056..348061,
348068..348070,348074..348076,348083..348094,
348098..348103,348110..348115,348119..348124,
348131..348136,348176..348178,348197..348202,
348212..348214,348221..348223,348308..348313,
348320..348322,348329..348334,348341..348343,
348629..348637,348641..348649,348653..348658)
/gene="prpB"
/locus_tag="b0331"
/gene_synonym="ECK0329; JW0323; yahQ"
/note="tetramer interface [polypeptide binding]; other
site"
/db_xref="CDD:119340"
misc_feature order(348032..348034,348038..348046,348077..348079,
348158..348160,348164..348166,348248..348250,
348377..348379,348467..348469,348533..348535,
348539..348541,348611..348613)
/gene="prpB"
/locus_tag="b0331"
/gene_synonym="ECK0329; JW0323; yahQ"
/note="active site"
/db_xref="CDD:119340"
misc_feature order(348077..348079,348158..348160,348164..348166,
348248..348250)
/gene="prpB"
/locus_tag="b0331"
/gene_synonym="ECK0329; JW0323; yahQ"
/note="Mg2+/Mn2+ binding site [ion binding]; other site"
/db_xref="CDD:119340"
repeat_region 348829..349193
/note="REP25 (repetitive extragenic palindromic) element;
contains 8 REP sequences"
gene 349236..350405
/gene="prpC"
/locus_tag="b0333"
/gene_synonym="ECK0330; JW0324; yahS; yzzD"
/db_xref="EcoGene:EG11756"
/db_xref="GeneID:947528"
CDS 349236..350405
/gene="prpC"
/locus_tag="b0333"
/gene_synonym="ECK0330; JW0324; yahS; yzzD"
/EC_number="2.3.3.1"
/function="putative enzyme; Not classified"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0019395 - fatty acid oxidation"
/note="putative citrate synthase; propionate metabolism?"
/codon_start=1
/transl_table=11
/product="2-methylcitrate synthase"
/protein_id="NP_414867.1"
/db_xref="GI:16128318"
/db_xref="ASAP:ABE-0001148"
/db_xref="UniProtKB/Swiss-Prot:P31660"
/db_xref="EcoGene:EG11756"
/db_xref="GeneID:947528"
/translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
RGYDILDLAKHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRPFVALDKRQ"
misc_feature 349263..350399
/gene="prpC"
/locus_tag="b0333"
/gene_synonym="ECK0330; JW0324; yahS; yzzD"
/note="Citrate synthase [Energy production and
conversion]; Region: GltA; COG0372"
/db_xref="CDD:223449"
misc_feature 349293..350390
/gene="prpC"
/locus_tag="b0333"
/gene_synonym="ECK0330; JW0324; yahS; yzzD"
/note="Subgroup of Escherichia coli (Ec) 2-methylcitrate
synthase (2MCS)_like. 2MCS catalyzes the condensation of
propionyl-coenzyme A (PrCoA) and oxalacetate (OAA) to form
2-methylcitrate and coenzyme A (CoA) during propionate
metabolism. Citrate synthase (CS)...; Region:
Ec2MCS_like_1; cd06117"
/db_xref="CDD:99870"
misc_feature order(349293..349301,349308..349322,349506..349511,
349518..349523,349545..349547,349557..349559,
349569..349571,349578..349580,349722..349724,
349833..349847,349854..349859,349869..349871,
349878..349889,349896..349898,349917..349922,
349926..349937,350058..350060,350331..350363)
/gene="prpC"
/locus_tag="b0333"
/gene_synonym="ECK0330; JW0324; yahS; yzzD"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:99870"
misc_feature order(349302..349304,349833..349835,349842..349844,
349935..349943,349947..349949,349956..349958,
350037..350060,350067..350069,350082..350084,
350187..350189,350193..350195,350202..350204,
350208..350210,350271..350273,350283..350285,
350331..350333,350340..350342)
/gene="prpC"
/locus_tag="b0333"
/gene_synonym="ECK0330; JW0324; yahS; yzzD"
/note="active site"
/db_xref="CDD:99870"
misc_feature order(349302..349304,349833..349835,349842..349844,
349935..349940,350037..350042,350046..350060,
350082..350084,350202..350204,350283..350285)
/gene="prpC"
/locus_tag="b0333"
/gene_synonym="ECK0330; JW0324; yahS; yzzD"
/note="citrylCoA binding site [chemical binding]; other
site"
/db_xref="CDD:99870"
misc_feature order(349833..349835,349842..349844,349938..349943,
350055..350057,350082..350084,350208..350210,
350271..350273,350283..350285,350340..350342)
/gene="prpC"
/locus_tag="b0333"
/gene_synonym="ECK0330; JW0324; yahS; yzzD"
/note="oxalacetate/citrate binding site [chemical
binding]; other site"
/db_xref="CDD:99870"
misc_feature order(349935..349940,349947..349949,349956..349958,
350037..350054,350058..350060,350067..350069,
350187..350189,350193..350195,350202..350204,
350208..350210,350331..350333)
/gene="prpC"
/locus_tag="b0333"
/gene_synonym="ECK0330; JW0324; yahS; yzzD"
/note="coenzyme A binding site [chemical binding]; other
site"
/db_xref="CDD:99870"
misc_feature order(349938..349940,350055..350057,350208..350210)
/gene="prpC"
/locus_tag="b0333"
/gene_synonym="ECK0330; JW0324; yahS; yzzD"
/note="catalytic triad [active]"
/db_xref="CDD:99870"
gene 350439..351890
/gene="prpD"
/locus_tag="b0334"
/gene_synonym="acnC; ECK0331; JW0325; yahT"
/db_xref="EcoGene:EG13603"
/db_xref="GeneID:945931"
CDS 350439..351890
/gene="prpD"
/locus_tag="b0334"
/gene_synonym="acnC; ECK0331; JW0325; yahT"
/EC_number="4.2.1.79"
/GO_process="GO:0016052 - carbohydrate catabolic process"
/codon_start=1
/transl_table=11
/product="2-methylcitrate dehydratase"
/protein_id="NP_414868.1"
/db_xref="GI:16128319"
/db_xref="ASAP:ABE-0001152"
/db_xref="UniProtKB/Swiss-Prot:P77243"
/db_xref="EcoGene:EG13603"
/db_xref="GeneID:945931"
/translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
misc_feature 350439..351887
/gene="prpD"
/locus_tag="b0334"
/gene_synonym="acnC; ECK0331; JW0325; yahT"
/note="2-methylcitrate dehydratase; Provisional; Region:
prpD; PRK09425"
/db_xref="CDD:181845"
misc_feature 350475..351887
/gene="prpD"
/locus_tag="b0334"
/gene_synonym="acnC; ECK0331; JW0325; yahT"
/note="2-methylcitrate dehydratase; Region: prpD;
TIGR02330"
/db_xref="CDD:131383"
gene 351930..353816
/gene="prpE"
/locus_tag="b0335"
/gene_synonym="ECK0332; JW0326; yahU"
/db_xref="EcoGene:EG13604"
/db_xref="GeneID:946891"
CDS 351930..353816
/gene="prpE"
/locus_tag="b0335"
/gene_synonym="ECK0332; JW0326; yahU"
/function="putative enzyme; Not classified"
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0019395 - fatty acid oxidation"
/note="putative propionyl-CoA synthetase"
/codon_start=1
/transl_table=11
/product="propionate--CoA ligase"
/protein_id="NP_414869.1"
/db_xref="GI:16128320"
/db_xref="ASAP:ABE-0001154"
/db_xref="UniProtKB/Swiss-Prot:P77495"
/db_xref="EcoGene:EG13604"
/db_xref="GeneID:946891"
/translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWTIVEKYQVSRMFSAP
TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
IQTIWGDDGRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
AMEE"
misc_feature 351930..353813
/gene="prpE"
/locus_tag="b0335"
/gene_synonym="ECK0332; JW0326; yahU"
/note="propionyl-CoA synthetase; Provisional; Region:
prpE; PRK10524"
/db_xref="CDD:182517"
misc_feature 351963..353795
/gene="prpE"
/locus_tag="b0335"
/gene_synonym="ECK0332; JW0326; yahU"
/note="Propionyl-CoA synthetase (PrpE); Region: PrpE;
cd05967"
/db_xref="CDD:213314"
misc_feature order(352638..352640,352647..352664,352668..352673)
/gene="prpE"
/locus_tag="b0335"
/gene_synonym="ECK0332; JW0326; yahU"
/note="acyl-activating enzyme (AAE) consensus motif; other
site"
/db_xref="CDD:213314"
misc_feature order(352647..352649,353013..353018,353079..353096,
353361..353363,353397..353399,353406..353408,
353439..353441,353703..353705)
/gene="prpE"
/locus_tag="b0335"
/gene_synonym="ECK0332; JW0326; yahU"
/note="putative AMP binding site [chemical binding]; other
site"
/db_xref="CDD:213314"
misc_feature order(352647..352649,352770..352775,352926..352928,
352932..352937,352944..352946,353013..353018,
353079..353096,353361..353363,353397..353399,
353406..353408,353430..353441,353646..353648)
/gene="prpE"
/locus_tag="b0335"
/gene_synonym="ECK0332; JW0326; yahU"
/note="putative active site [active]"
/db_xref="CDD:213314"
misc_feature order(352770..352772,352932..352937,352944..352946,
353013..353015,353430..353438,353628..353630,
353646..353648)
/gene="prpE"
/ 