LOCUS NC_003745 4579 bp ds-RNA linear VRL 30-NOV-2009
DEFINITION Saccharomyces cerevisiae virus L-A (L1), complete genome.
ACCESSION NC_003745
VERSION NC_003745.1 GI:20428567
DBLINK Project:14792
KEYWORDS .
SOURCE Saccharomyces cerevisiae virus L-A (L1)
ORGANISM Saccharomyces cerevisiae virus L-A (L1)
Viruses; dsRNA viruses; Totiviridae; Totivirus.
REFERENCE 1 (bases 1 to 4579)
AUTHORS Icho,T. and Wickner,R.B.
TITLE The double-stranded RNA genome of yeast virus L-A encodes its own
putative RNA polymerase by fusing two open reading frames
JOURNAL J. Biol. Chem. 264 (12), 6716-6723 (1989)
PUBMED 2651431
REFERENCE 2 (bases 1 to 4579)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (03-MAY-2002) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence is identical to J04692.
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..4579
/organism="Saccharomyces cerevisiae virus L-A (L1)"
/mol_type="genomic RNA"
/db_xref="taxon:11008"
/clone="pTIL05"
gene 30..4546
/locus_tag="ScVLAgp1"
/db_xref="GeneID:940478"
CDS join(30..1961,1961..4546)
/locus_tag="ScVLAgp1"
/codon_start=1
/product="ssRNA-binding protein"
/protein_id="NP_620493.1"
/db_xref="GI:20428569"
/db_xref="GeneID:940478"
/translation="MLRFVTKNSQDKSSDLFSICSDRGTFVAHNRVRTDFKFDNLVFN
RVYGVSQKFTLVGNPTVCFNEGSSYLEGIAKKYLTLDGGLAIDNVLNELRSTCGIPGN
AVASHAYNITSWRWYDNHVALLMNMLRAYHLQVLTEQGQYSAGDIPMYHDGHVKIKLP
VTIDDTAGPTQFAWPSDRSTDSYPDWAQFSESFPSIDVPYLDVRPLTVTEVNFVLMMM
SKWHRRTNLAIDYEAPQLADKFAYRHALTVQDADEWIEGDRTDDQFRPPSSKVMLSAL
RKYVNHNRLYNQFYTAAQLLAQIMMKPVPNCAEGYAWLMHDALVNIPKFGSIRGRYPF
LLSGDAALIQATALEDWSAIMAKPELVFTYAMQVSVALNTGLYLRRVKKTGFGTTIDD
SYEDGAFLQPETFVQAALACCTGQDAPLNGMSDVYVTYPDLLEFDAVTQVPITVIEPA
GYNIVDDHLVVVGVPVACSPYMIFPVAAFDTANPYCGNFVIKAANKYLRKGAVYDKLE
AWKLAWALRVAGYDTHFKVYGDTHGLTKFYADNGDTWTHIPEFVTDGDVMEVFVTAIE
RRARHFVELPRLNSPAFFRSVEVSTTIYDTHVQAGAHAVYHASRINLDYVKPVSTGIQ
VINAGELKNYWGSVRRTQQGFRSGRSYDASCNAYRRTYSWRCPRRVDRTGGQCFSRVN
VIEPSHGPRPTRYILQEPGTYPAWIRFRNRVQAVSRQKATHFLFDIVPAAVISDFTTS
DTSSFAYKSHTYAVNVTALRFSDTYALYVQTDTNMTILSPAARRQASATYSQVAGFCY
NTPTVMDSLANILDVDRNIRPKHFKGLRLYTRSKVTAQHHTHLRPDELVEAAAKVSPR
RKYYLMCVVELLANLQVDLEAAVATILAYVLTLSEKFVPIFLDSRAIWVGEPGPDALT
ARLKASSGQIKSIHTADYEPLTELFELAVLMNRGVGHVSWQAEKDHRLNPDVAVVDQA
RLYSCVRDMFEGSKQTYKYPFMTWDDYTANRWEWVPGGSVHSQYEEDNDYIYPGQYTR
NKFITVNKMPKHKISRMIASPPEVRAWTSTKYEWGKQRAIYGTDLRSTLITNFAMFRC
EDVLTHKFPVGDQAEAAKVHKRVNMMLDGASSFCFDYDDFNSQHSIASMYTVLCAFRD
TFSRNMSDEQAEAMNWVCESVRHMWVLDPDTKEWYRLQGTLLSGWRLTTFMNTVLNWA
YMKLAGVFDLDDVQDSVHNGDDVMISLNRVSTAVRIMDAMHRINARAQPAKCNLFSIS
EFLRVEHGMSGGDGLGAQYLSRSCATLVHSRIESNEPLSVVRVMEADQARLRDLANRT
RVQSAVTAIKEQLDKRVTKIFGVGDDVVRDIHTAHRVCGGISTDTWAPVETKIITDNE
AYEIPYEIDDPSFWPGVNDYAYKVWKNFGERLEFNKIKDAVARGSRSTIALKRKARIT
SKKNEFANKSEWERTMYKAYKGLAVSYYANLSKFMSIPPMANIEFGQARYAMQAALDS
SDPLRALQVIL"
misc_feature 30..1337
/locus_tag="ScVLAgp1"
/note="L-A virus, major coat protein; Region:
LA-virus_coat; pfam09220"
/db_xref="CDD:150036"
misc_feature 3206..3832
/locus_tag="ScVLAgp1"
/note="RT_like: Reverse transcriptase (RT, RNA-dependent
DNA polymerase)_like family. An RT gene is usually
indicative of a mobile element such as a retrotransposon
or retrovirus. RTs occur in a variety of mobile elements,
including retrotransposons...; Region: RT_like; cl02808"
/db_xref="CDD:174733"
misc_feature order(3398..3415,3593..3598,3695..3697,3701..3706)
/locus_tag="ScVLAgp1"
/note="active site"
/db_xref="CDD:73150"
misc_feature order(3398..3415,3593..3595,3701..3703)
/locus_tag="ScVLAgp1"
/note="NTP binding site; other site"
/db_xref="CDD:73150"
misc_feature 3596..3598
/locus_tag="ScVLAgp1"
/note="nucleic acid binding site; other site"
/db_xref="CDD:73150"
gene 30..2072
/locus_tag="ScVLAgp2"
/db_xref="GeneID:940477"
CDS 30..2072
/locus_tag="ScVLAgp2"
/codon_start=1
/product="major coat protein"
/protein_id="NP_620494.1"
/db_xref="GI:20428568"
/db_xref="GeneID:940477"
/translation="MLRFVTKNSQDKSSDLFSICSDRGTFVAHNRVRTDFKFDNLVFN
RVYGVSQKFTLVGNPTVCFNEGSSYLEGIAKKYLTLDGGLAIDNVLNELRSTCGIPGN
AVASHAYNITSWRWYDNHVALLMNMLRAYHLQVLTEQGQYSAGDIPMYHDGHVKIKLP
VTIDDTAGPTQFAWPSDRSTDSYPDWAQFSESFPSIDVPYLDVRPLTVTEVNFVLMMM
SKWHRRTNLAIDYEAPQLADKFAYRHALTVQDADEWIEGDRTDDQFRPPSSKVMLSAL
RKYVNHNRLYNQFYTAAQLLAQIMMKPVPNCAEGYAWLMHDALVNIPKFGSIRGRYPF
LLSGDAALIQATALEDWSAIMAKPELVFTYAMQVSVALNTGLYLRRVKKTGFGTTIDD
SYEDGAFLQPETFVQAALACCTGQDAPLNGMSDVYVTYPDLLEFDAVTQVPITVIEPA
GYNIVDDHLVVVGVPVACSPYMIFPVAAFDTANPYCGNFVIKAANKYLRKGAVYDKLE
AWKLAWALRVAGYDTHFKVYGDTHGLTKFYADNGDTWTHIPEFVTDGDVMEVFVTAIE
RRARHFVELPRLNSPAFFRSVEVSTTIYDTHVQAGAHAVYHASRINLDYVKPVSTGIQ
VINAGELKNYWGSVRRTQQGLGVVGLTMPAVMPTGEPTAGAAHEELIEQADNVLVE"
misc_feature 30..1337
/locus_tag="ScVLAgp2"
/note="L-A virus, major coat protein; Region:
LA-virus_coat; pfam09220"
/db_xref="CDD:150036"
gene 2351..4546
/locus_tag="ScVLAgp3"
/db_xref="GeneID:940476"
CDS 2351..4546
/locus_tag="ScVLAgp3"
/note="RNA-dependent RNA polymerase of icosahedral RNA
viruses; putative"
/codon_start=1
/product="RNA polymerase"
/protein_id="NP_620495.1"
/db_xref="GI:20428570"
/db_xref="GeneID:940476"
/translation="MTILSPAARRQASATYSQVAGFCYNTPTVMDSLANILDVDRNIR
PKHFKGLRLYTRSKVTAQHHTHLRPDELVEAAAKVSPRRKYYLMCVVELLANLQVDLE
AAVATILAYVLTLSEKFVPIFLDSRAIWVGEPGPDALTARLKASSGQIKSIHTADYEP
LTELFELAVLMNRGVGHVSWQAEKDHRLNPDVAVVDQARLYSCVRDMFEGSKQTYKYP
FMTWDDYTANRWEWVPGGSVHSQYEEDNDYIYPGQYTRNKFITVNKMPKHKISRMIAS
PPEVRAWTSTKYEWGKQRAIYGTDLRSTLITNFAMFRCEDVLTHKFPVGDQAEAAKVH
KRVNMMLDGASSFCFDYDDFNSQHSIASMYTVLCAFRDTFSRNMSDEQAEAMNWVCES
VRHMWVLDPDTKEWYRLQGTLLSGWRLTTFMNTVLNWAYMKLAGVFDLDDVQDSVHNG
DDVMISLNRVSTAVRIMDAMHRINARAQPAKCNLFSISEFLRVEHGMSGGDGLGAQYL
SRSCATLVHSRIESNEPLSVVRVMEADQARLRDLANRTRVQSAVTAIKEQLDKRVTKI
FGVGDDVVRDIHTAHRVCGGISTDTWAPVETKIITDNEAYEIPYEIDDPSFWPGVNDY
AYKVWKNFGERLEFNKIKDAVARGSRSTIALKRKARITSKKNEFANKSEWERTMYKAY
KGLAVSYYANLSKFMSIPPMANIEFGQARYAMQAALDSSDPLRALQVIL"
misc_feature 3206..3832
/locus_tag="ScVLAgp3"
/note="RT_like: Reverse transcriptase (RT, RNA-dependent
DNA polymerase)_like family. An RT gene is usually
indicative of a mobile element such as a retrotransposon
or retrovirus. RTs occur in a variety of mobile elements,
including retrotransposons...; Region: RT_like; cl02808"
/db_xref="CDD:174733"
misc_feature order(3398..3415,3593..3598,3695..3697,3701..3706)
/locus_tag="ScVLAgp3"
/note="active site"
/db_xref="CDD:73150"
misc_feature order(3398..3415,3593..3595,3701..3703)
/locus_tag="ScVLAgp3"
/note="NTP binding site; other site"
/db_xref="CDD:73150"
misc_feature 3596..3598
/locus_tag="ScVLAgp3"
/note="nucleic acid binding site; other site"
/db_xref="CDD:73150"
ORIGIN
1 gaaaaatttt taaattcata taactcccca tgctaagatt tgttactaaa aactctcaag
61 ataaatcgtc tgatctattc tctatttgtt ctgatcgcgg aacttttgtt gcccataata
121 gagttcggac tgatttcaag tttgacaact tagtattcaa ccgagtttat ggtgtttctc
181 aaaaatttac gttagtcggt aacccgacag tctgctttaa tgaaggcagt tcttacctag
241 aaggtattgc taaaaagtac ctaactttgg atggaggact tgccattgac aatgtcctca
301 atgagttgag atccacttgc ggtatcccag gtaatgctgt tgcatctcat gcgtataata
361 ttacatcctg gcgttggtat gacaatcacg tggcactgtt gatgaacatg ttgcgtgctt
421 accacttaca agtattgacc gaacagggcc aatatagcgc tggagatatc cctatgtacc
481 atgatggaca tgtcaaaatc aagctaccag tgactatcga tgacacggca ggcccaacac
541 aattcgcttg gcctagtgac aggtctactg attcgtatcc tgattgggca cagttttctg
601 aatcatttcc atcaatcgac gtcccgtacc tagatgttag gccattgacc gtaacggaag
661 tcaatttcgt gcttatgatg atgagtaagt ggcatagacg tactaactta gcgatagact
721 acgaggcacc ccaactagct gataagttcg cttaccgcca tgcgcttact gttcaagacg
781 ctgacgagtg gatagaaggc gatagaactg atgaccagtt ccgccccccc tcgtctaaag
841 taatgttatc ggcacttcgt aagtacgtga accataacag gctgtacaat cagttttaca
901 ctgcagcaca actgttagct caaattatga tgaaacctgt ccctaactgc gctgagggct
961 acgcttggct gatgcatgac gcattggtca atataccaaa atttgggtct attcgaggaa
1021 ggtacccctt tttgttatca ggtgatgcag cgttgattca ggctacagcc ctagaagact
1081 ggtctgctat catggcgaaa cccgagctgg tgttcactta cgcgatgcag gtgtcagtag
1141 cgttaaacac cggactatac ttacgtcgcg ttaagaaaac aggcttcggc acaactatag
1201 atgacagcta tgaagatgga gcgtttttgc aaccggagac gttcgttcag gccgcactag
1261 catgttgtac cggacaagat gcgcccctaa atgggatgtc agatgtgtat gtcacttatc
1321 cagatcttct agaatttgat gctgttacac aagtacccat cacggtcatt gagcccgctg
1381 gctataacat tgttgatgat catttagtgg ttgtgggtgt acctgtggca tgttcaccat
1441 acatgatatt tccagtagct gcgtttgata ctgcaaatcc ttactgtggg aattttgtca
1501 ttaaggctgc taacaagtat ctccgtaagg gtgccgtgta tgataaactc gaagcatgga
1561 agttggcctg ggcactgagg gtagccgggt atgacactca cttcaaagtg tatggcgata
1621 cacacggctt aactaagttc tatgctgaca acggtgacac atggacacac atacctgaat
1681 ttgtcactga cggtgacgtg atggaagtat tcgttactgc catcgaacgc agagctagac
1741 atttcgttga actacctaga ctgaattcac cagcattctt cagatctgta gaagtcagca
1801 ccactatata tgatactcat gtgcaggctg gtgcgcatgc ggtgtatcat gctagtcgaa
1861 tcaatcttga ttatgttaag cctgtttcga ccggcattca ggtgatcaat gcgggcgaac
1921 ttaagaacta ctggggtagt gtgcgtcgta ctcagcaggg tttaggagtg gtaggtctta
1981 cgatgccagc tgtaatgcct accggagaac ctacagctgg cgctgcccac gaagagttga
2041 tagaacaggc ggacaatgtt ttagtagagt aaacgtaatc gaaccctcac acggaccccg
2101 ccctacaagg tacatactgc aggaaccagg tacgtaccct gcgtggatta gattcaggaa
2161 cagagtacaa gctgtatcgc gtcagaaagc cactcacttc ttgtttgaca tcgtacctgc
2221 cgcagtaatt agtgatttta ctacgtctga cacgtcttcg tttgcataca aatcgcacac
2281 ctacgctgta aatgtaacag cattgaggtt cagtgacact tatgccttgt acgtacagac
2341 tgataccaac atgacaattt taagcccagc ggcgcgtcgc caggcttctg cgacgtactc
2401 acaggtggca gggttttgtt ataacacacc taccgttatg gattcgctag cgaatatctt
2461 ggacgtagac cgcaatatac gacccaaaca cttcaagggt ttacggctat acacaaggtc
2521 taaggtcact gctcaacatc atactcactt gcggccagac gagctagtgg aagcggccgc
2581 aaaggtctcg cctagacgta aatactactt aatgtgtgta gttgagctgc tcgcgaactt
2641 acaagtagat cttgaagcag cagtagctac tattctcgca tatgtcctga cactaagtga
2701 aaaatttgta ccaattttct tggattctag agcaatatgg gtcggtgagc ctgggcctga
2761 tgctctgact gcacgtctca aggccagtag tgggcagatc aagagcatac acacggctga
2821 ttacgaacca ctcactgaac tattcgagtt agcagtattg atgaaccgag gtgttggcca
2881 tgtctcttgg caagctgaaa aggatcatcg cttgaatccc gacgtggctg tagttgatca
2941 agcacggcta tattcgtgtg tgcgcgacat gttcgaagga tcaaagcaga cgtataaata
3001 tccctttatg acgtgggatg actacactgc aaacagatgg gagtgggttc caggtggcag
3061 tgtccactct caatacgaag aagacaacga ttatatctat cctggtcagt atactaggaa
3121 caagttcata actgttaaca aaatgcccaa acacaaaata tctagaatga tagcatcacc
3181 gcctgaggta cgagcttgga cgtcgacgaa gtacgaatgg ggcaagcaac gtgctatcta
3241 cgggacggat ctacgaagta cactgataac taactttgca atgttcaggt gcgaggatgt
3301 tctcactcac aagttcccag taggcgacca ggcagaggca gcaaaggtgc acaaacgggt
3361 gaacatgatg ctggacggtg cctctagttt ctgcttcgat tatgatgact tcaattctca
3421 gcattcaata gctagtatgt atacggtttt gtgcgctttc agggacacat ttagtcgcaa
3481 catgtctgat gaacaagcag aggcgatgaa ctgggtgtgt gagtccgtca gacacatgtg
3541 ggtactagat cctgatacca aggagtggta cagactacaa ggtacattac tgtcaggatg
3601 gcggttaacc acatttatga acactgtgct aaactgggcg tatatgaaat tagctggcgt
3661 atttgatctg gatgacgttc aagactcggt acacaacggt gatgatgtta tgattagtct
3721 caaccgcgtg agcacagcag taagaataat ggacgctatg caccggataa atgcgcgagc
3781 acagccggcg aagtgtaact tgttttcgat aagtgaattt ctgagggtag aacacggtat
3841 gagcggaggc gatggtcttg gggctcagta cttaagtagg tcttgtgcta ctcttgtaca
3901 cagtaggatt gagtctaacg aaccactgtc agtagtacga gttatggaag cagaccaggc
3961 tagattgcgc gacctggcaa acagaacgcg ggtacaatct gcggtaacag cgataaaaga
4021 acaactcgac aaacgtgtca ctaagatatt cggagttggt gatgacgttg tgcgcgacat
4081 acacacagct cacagggtgt gtggcggtat ctcgactgat acctgggcac cggttgaaac
4141 taagataata acagacaatg aagcatatga aataccatac gaaatagatg atccatcatt
4201 ttggccaggg gtaaatgatt atgcttataa agtctggaaa aatttcggag aacgactcga
4261 atttaataag attaaagatg ccgtagctag agggagtagg agcactatag ctctgaaacg
4321 taaggctagg ataacatcta agaagaatga attcgctaac aagtcggaat gggaaaggac
4381 aatgtacaaa gcctataagg gtttggcagt ctcatactat gctaacctga gcaaattcat
4441 gagtatacca ccaatggcga acattgaatt tgggcaggct agatatgcta tgcaagcagc
4501 ccttgatagt tctgatccac tccgggcatt acaggtcata ctgtaattgc caaaaagata
4561 atgggaatta cccatatgc
//
