LOCUS NC_013657 37510 bp DNA circular CON 22-FEB-2023
DEFINITION Lactococcus lactis subsp. lactis KF147 plasmid pKF147A, complete
sequence.
ACCESSION NC_013657
VERSION NC_013657.1
DBLINK BioProject: PRJNA224116
BioSample: SAMN02603087
Assembly: GCF_000025045.1
KEYWORDS RefSeq.
SOURCE Lactococcus lactis subsp. lactis KF147
ORGANISM Lactococcus lactis subsp. lactis KF147
Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
Lactococcus.
REFERENCE 1 (bases 1 to 37510)
AUTHORS Siezen,R.J., Bayjanov,J., Renckens,B., Wels,M., van Hijum,S.A.,
Molenaar,D. and van Hylckama Vlieg,J.E.
TITLE Complete genome sequence of Lactococcus lactis subsp. lactis KF147,
a plant-associated lactic acid bacterium
JOURNAL J Bacteriol 192 (10), 2649-2650 (2010)
PUBMED 20348266
REFERENCE 2 (bases 1 to 37510)
AUTHORS Siezen,R.J., Bayjanov,J., Renckens,B.A.M., Wels,M., van
Hijum,S.A.F.T., Molenaar,D. and van Hylckama Vlieg,J.E.T.
TITLE Complete genome sequence of Lactococcus lactis ssp lactis KF147
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 37510)
AUTHORS Siezen,R.J., Bayjanov,J., Renckens,B.A.M., Wels,M., van
Hijum,S.A.F.T., Molenaar,D. and van Hylckama Vlieg,J.E.T.
TITLE Direct Submission
JOURNAL Submitted (01-DEC-2009) NIZO Food Research, Kluyver Centre for
Genomics of Industrial Fermentation, PO Box 20, Ede 6710 BA, The
Netherlands
COMMENT REFSEQ INFORMATION: The reference sequence is identical to
CP001835.1.
Bacteria source: Dr. W.J. Kelly, Agresearch Limited, New Zealand;
william.kelly@agresearch.co.nz.
The annotation was added by the NCBI Prokaryotic Genome Annotation
Pipeline (PGAP). Information about PGAP can be found here:
https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI RefSeq
Annotation Date :: 02/22/2023 13:53:56
Annotation Pipeline :: NCBI Prokaryotic Genome
Annotation Pipeline (PGAP)
Annotation Method :: Best-placed reference protein
set; GeneMarkS-2+
Annotation Software revision :: 6.4
Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA
Genes (total) :: 2,639
CDSs (total) :: 2,548
Genes (coding) :: 2,506
CDSs (with protein) :: 2,506
Genes (RNA) :: 91
rRNAs :: 7, 6, 6 (5S, 16S, 23S)
complete rRNAs :: 7, 6, 6 (5S, 16S, 23S)
tRNAs :: 68
ncRNAs :: 4
Pseudo Genes (total) :: 42
CDSs (without protein) :: 42
Pseudo Genes (ambiguous residues) :: 0 of 42
Pseudo Genes (frameshifted) :: 20 of 42
Pseudo Genes (incomplete) :: 14 of 42
Pseudo Genes (internal stop) :: 13 of 42
Pseudo Genes (multiple problems) :: 3 of 42
##Genome-Annotation-Data-END##
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..37510
/organism="Lactococcus lactis subsp. lactis KF147"
/mol_type="genomic DNA"
/strain="KF147"
/isolation_source="sprouts"
/host="mung bean"
/sub_species="lactis"
/db_xref="taxon:684738"
/plasmid="pKF147A"
gene 582..1934
/locus_tag="LLKF_RS12815"
/old_locus_tag="LLKF_p0001"
CDS 582..1934
/locus_tag="LLKF_RS12815"
/old_locus_tag="LLKF_p0001"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_011915264.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="replication initiation protein"
/protein_id="WP_011915264.1"
/translation="MQSYDLLDELDSEDKFRKDIKYSRQLPEMFSTEDINAASENITY
AILGELRDRYNGSEPVTFSYQELAELGGLWVTRKNGVKSLYNGKRLQKIMYDLNEALK
NFSYYQVRETNDDGTPKSWKTINIFSVIDFDGTKKEVKLTISNAQISSEQVDAKGHVI
DKPLYVYDLINSKDWRTVKHLQYNRGINNSLPSKYSKRVYRFISEFRSFPKGTKMRID
DFDKKILKILKTQEDSFNTKEVFDLRKNRKKYLETAVKEISELTTPEGTQIVKNLDYI
YHTSGRRIQSIEFTYTPFNADLSGSNHISMNSRTSSPGTASPFINEARMVLEYFNYLS
KVNFNLDENGIIKHLPNYYDIQFELDDIQLLQPIHKLLESGVAIDELLQVAEMKAIDW
KLDSNQMINNFRPSVVFGNKFSEYRAFLTTYKAQNIHKLVFDSSSDFYVPMNGPWDSK
"
gene 2448..2681
/locus_tag="LLKF_RS12820"
CDS 2448..2681
/locus_tag="LLKF_RS12820"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_014573541.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="WP_014573541.1"
/translation="MEKVTDEIKNVVQRLLDDDENFSGWYIEKELEKIGIKVSRMTIS
NLRNKKTTLGNTKFETLEGLYHFAKTHENINKE"
gene 2691..3449
/locus_tag="LLKF_RS12825"
/old_locus_tag="LLKF_p0002"
CDS 2691..3449
/locus_tag="LLKF_RS12825"
/old_locus_tag="LLKF_p0002"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_012898729.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="AAA family ATPase"
/protein_id="WP_012898729.1"
/translation="MKTISLLNLKGGVAKTTTGGNIAKGLANRGFKTLLIDTDMQANA
TSIFLEDERSKEDYKDFAELIVDEKLEDVDQYVYNVSENLDMIGSSLAVAESELKVRN
SFNRNSSNIVKKVLKKLDSKYDYCIIDCAPTINLITLNIIIASDEIIIPIKIDKFALE
GYRTTLKNINQIIDDYELDTEVTVLYTMVNRNNIDKQFIQEISGNRFETTIRHQAKPV
TESALKNEVLIDSSKSSKVKDDYLNLIDEIVKRG"
gene 3453..4181
/locus_tag="LLKF_RS12830"
/old_locus_tag="LLKF_p0003"
CDS 3453..4181
/locus_tag="LLKF_RS12830"
/old_locus_tag="LLKF_p0003"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_017865306.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="ParB N-terminal domain-containing protein"
/protein_id="WP_011915262.1"
/translation="MSNSFGFTDLMNKDEHKRKKTNTKNIPIEEIKENENNNYDLVDI
DKLADSIDELGLLQPVLVKQRDKYSYELIAGHRRFNAIKKLISENRLPEDYEVLAKKV
DEDEDELVTRLKLHETNLQTRSLLKMPEEEKIAIIDDYMDILDKAKKQGVQINGKPVK
GKTRDLIAERFGISHYTAQKLIRKAKEQGGEEEGAKISPQKKTAKKPITQLKKIETQL
EKLEFEGTEEEQEIKKKLIELLMK"
gene complement(4211..4561)
/locus_tag="LLKF_RS12835"
/old_locus_tag="LLKF_p0004"
CDS complement(4211..4561)
/locus_tag="LLKF_RS12835"
/old_locus_tag="LLKF_p0004"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_014011586.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="WP_012898730.1"
/translation="MSVDRSYSPYEVIRAYHDRGMMKWGAFATGELTEAQNTFEKEKK
EDKKIQALPHHLVLYLLNQSFSNQLQIKVKYQSKNKLTEVYGFISEFINNQVRVKSTD
KIYLISIEQIINIS"
gene complement(4551..6017)
/locus_tag="LLKF_RS12840"
/old_locus_tag="LLKF_p0005"
CDS complement(4551..6017)
/locus_tag="LLKF_RS12840"
/old_locus_tag="LLKF_p0005"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_015425922.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="Y-family DNA polymerase"
/protein_id="WP_012898731.1"
/translation="MEQLKLNQYFDYSLEPRRAILFQDVKSNYASIECVQRNLNPLTT
SLCVMSRADHSKGLTLASSPTFKKVFGMKNVSRASDLPFLIETRKFNYPQWYRTHTDI
HGQRTEPTLQYVAFIESWAKRTWIVPPQMQLYVDYKIEVTDILTNYTSIDEIHSYSID
ESFLDITESLNFFYSEIKNRYEQMNRIALDLQREIRDKLGLYVTVGMGDNPLLAKLAM
DNYAKHNDNMRALIRYEDVPNKLWTIPKMTDFWGIGKRTEKRLNKLGITSIKELANAD
PLLLKQNLGTIGLQHFFHANGIDESNVREKYVPKTTSFSNSQILPRDYHKQREIELVI
KEMAENLAIRLRKGGKLASNLSLYAGAASTSEYSSVKVSRNIEATQNTKELQDLAISL
FREKYQGGAIRQIGISGNQLSDSSVKQLSLFESVEENQTNKKQGLLQKVIDEIRETFD
FLSIQKASSLSEGSRVIYRNKLIGGHAASQEREEKDVS"
gene 6432..7052
/locus_tag="LLKF_RS12845"
/old_locus_tag="LLKF_p0006"
CDS 6432..7052
/locus_tag="LLKF_RS12845"
/old_locus_tag="LLKF_p0006"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_076611694.1"
/GO_function="GO:0004803 - transposase activity [Evidence
IEA]"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="IS6 family transposase"
/protein_id="WP_148210857.1"
/translation="MIVAVGYYLRYNLSYREVQELLYDRGINVCHTKIYRWVQEYSKV
LYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIWLRKKRDTQAAYA
FLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKYLNNLIEQDHRPI
KRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTFIV"
gene 7340..7951
/locus_tag="LLKF_RS13080"
/old_locus_tag="LLKF_p0007"
CDS 7340..7951
/locus_tag="LLKF_RS13080"
/old_locus_tag="LLKF_p0007"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_012898732.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="WP_050749812.1"
/translation="MKINDGEKLVMKLSKVHCKECGGILNLDIVSHIKNQKLVCPYCQ
SLYIYEAKYSEIGAELEADIELIRLKEEKENIKEFWKFKKLKEDHKVGFISLLILFSI
PLIGFLVMTTNYLIVHRPGQIELPISEKKLHGKNYKNVELKFEDMGFENIKYEKVRDL
KLGLFAHSGDVSEVTINGDNDFKKGDNYNKKSKIKIYYHVFPK"
gene 8374..8535
/locus_tag="LLKF_RS13665"
CDS 8374..8535
/locus_tag="LLKF_RS13665"
/inference="COORDINATES: ab initio
prediction:GeneMarkS-2+"
/note="Derived by automated computational analysis using
gene prediction method: GeneMarkS-2+."
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="WP_153927130.1"
/translation="MNKINTIAILKAPKIIVLVESDLVSLENLAIITALRMKHRNIKA
KNIVGEMPT"
gene 9385..9534
/locus_tag="LLKF_RS13325"
CDS 9385..9534
/locus_tag="LLKF_RS13325"
/inference="COORDINATES: protein motif:HMM:NF016272.2"
/GO_component="GO:0005576 - extracellular region [Evidence
IEA]"
/GO_process="GO:0042742 - defense response to bacterium
[Evidence IEA]"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="lactococcin family bacteriocin"
/protein_id="WP_017865277.1"
/translation="MENQTNFEVVSDEELSKINGGVALELTREGYYNQYGGPLLYENR
PLLEY"
gene 9645..10349
/locus_tag="LLKF_RS12865"
/old_locus_tag="LLKF_p0010"
CDS 9645..10349
/locus_tag="LLKF_RS12865"
/old_locus_tag="LLKF_p0010"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_017865276.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="type II CAAX endopeptidase family protein"
/protein_id="WP_012898735.1"
/translation="MKTIKKYKLNFFYLLLFVLLHTLYKIPGYLVFAYYLDNPLILPF
TDFLYTNSALAFPILLTLVAVIVIIIPYYLGFFKNTFEGWSTIRVFRLLVILFVLLLL
IYFTNVLMIRLGFSSSNHVGRSQNYLITMPKVMQYYIIGIVAPIIEEILWRVFLFKAV
GNKNLALFVSWPLFAFAHTGLSLEIIPYLFLSFIVTWAYYRNENVLESIFIHASYNLL
SPLSAQFLNSFFHFIV"
gene complement(10580..11176)
/locus_tag="LLKF_RS12870"
/old_locus_tag="LLKF_p0011"
CDS complement(10580..11176)
/locus_tag="LLKF_RS12870"
/old_locus_tag="LLKF_p0011"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_003133553.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="helix-turn-helix transcriptional regulator"
/protein_id="WP_012898736.1"
/translation="MELSTILVEERKKKGITQQEVADTLYITRQSLSNWENGKNFPDI
PMLVELSKYYDFSLDIIKGDAQLMNQVQKDYELINTKKANKKYSVLLVILTALIVLTS
VVLIPLVSNNKILLKSFTLFDLLLCIVLIHTAVKFSKIVYQYYEGIPNSPLWVPKVFG
YGISINPYNKFGKIITIALLVILDLFFIGVGITMIFFG"
gene 11486..11905
/locus_tag="LLKF_RS12875"
/old_locus_tag="LLKF_p0012"
CDS 11486..11905
/locus_tag="LLKF_RS12875"
/old_locus_tag="LLKF_p0012"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_002338443.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="DDE-type integrase/transposase/recombinase"
/protein_id="WP_012898737.1"
/translation="MNHFKGKQFQKDVIIISVGYYLRYNLSYRDVQEMLYDRGINVSH
TTIYRWVQEYGKLLYQNGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASPT
IKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
gene complement(12871..13494)
/locus_tag="LLKF_RS12880"
/old_locus_tag="LLKF_p0014"
CDS complement(12871..13494)
/locus_tag="LLKF_RS12880"
/old_locus_tag="LLKF_p0014"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_010749140.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="DUF2812 domain-containing protein"
/protein_id="WP_012898739.1"
/translation="MKKFRIFLSLKKEEEWINSIQEEGYKLVSVNSAVPMYTFEKLST
KEMFIPYVRLDFREKSMKKTNYEDYVTLFSDSGWKLIKGSRSGGAQYFQQKRPDVSRG
IFSDKDSQESAKRRYVKYGYSYGFLFLLYFYIFSRNTSWTQIINFKSWYLTPNIWNLK
GGSFWYAFLFETPFALFRVLPLFFFLFLGIYYLIRSLINDDSKFKRK"
gene complement(13506..13817)
/locus_tag="LLKF_RS12885"
/old_locus_tag="LLKF_p0015"
CDS complement(13506..13817)
/locus_tag="LLKF_RS12885"
/old_locus_tag="LLKF_p0015"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_002291321.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="PadR family transcriptional regulator"
/protein_id="WP_012898740.1"
/translation="MSRNPHLPLTETTFYILLVLTTPAHGYAIIQEIEKLSNGFVKVA
AGTMYGAIENLLKLNWIEEIPNREKRRRVYKITDIGEEVLTLEIERLRSLIKLSSEFG
Y"
gene 14210..14764
/locus_tag="LLKF_RS12890"
/old_locus_tag="LLKF_p0016"
CDS 14210..14764
/locus_tag="LLKF_RS12890"
/old_locus_tag="LLKF_p0016"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_003106713.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="recombinase family protein"
/protein_id="WP_012898741.1"
/translation="MNIGYARVSTGLQNLDLQKDSLEKYNCEKIFTDHMSGSKKERPG
LKSAVEFSRPGDTIVVWRLDRLGRNMEDLINIVNSLNNKGVSFHSLQENITMDKSSST
GQLMFHLFAAFAEFERNLILERSAAGREAARARGRLGGRPEKFSEQDIKLLKTLVESG
TPIKSIADSWGVSRTTIYRYINKF"
gene complement(14956..16066)
/locus_tag="LLKF_RS13440"
/pseudo
CDS complement(14956..16066)
/locus_tag="LLKF_RS13440"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_086896442.1"
/GO_function="GO:0004803 - transposase activity [Evidence
IEA]"
/note="frameshifted; Derived by automated computational
analysis using gene prediction method: Protein Homology."
/pseudo
/codon_start=1
/transl_table=11
/product="IS3 family transposase"
gene 16463..18490
/locus_tag="LLKF_RS12905"
/old_locus_tag="LLKF_p0020"
CDS 16463..18490
/locus_tag="LLKF_RS12905"
/old_locus_tag="LLKF_p0020"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_003136019.1"
/GO_component="GO:0016020 - membrane [Evidence IEA]"
/GO_function="GO:0015079 - potassium ion transmembrane
transporter activity [Evidence IEA]"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="KUP/HAK/KT family potassium transporter"
/protein_id="WP_012898743.1"
/translation="MNQIKSRSFNKATSAGFIVALGIIYGDIGTSPLYTMQSILESQG
GIEHIEKASIIGSISLIIWTLTLITTVKYVLIALRADNNHEGGIFSLFTLVRKYAKWL
IIPAMIGGAALLSDGALTPAVTVTSAIEGLRTIPAFETTFGSRQIPIILITLIILALL
FSIQRFGTAVVGKIFGPIMLIWFSFLGFFGFINLCGTPSILQAINPYYAIHFLFSPDN
KSGIFILGSIFLATTGAEALYSDLGHVGRGNIYISWPFVKICILLSYCGQASWILKNQ
GRNLGIINPFFSILPGYIIIFAVILATLAAIIASQALISGSFSLVSEAIRLKLFPLLK
IYYPGKTVGQLYIPAINLGLWIASSFIVIYFQTSAHMEAAYGLAITITMLMTTTLLTF
YLSHHQEIKKMLVILFFSTFILVEGLFFVASIVKFIHGGYIVVVFASIILFIMAIWHK
ATQLTFKYIKSLNINCYKHQLNILRKDESYDLYQTNVVYLTSRVDGEWIDRSILYSLL
DKKPKRSEIYWFVNIRVTDEPYTSEYKIDMFETDYIVSVTLFLGFRMRQDVPRYLRTI
VTDLMESGRIHRQYQRYSITPNREVGDFRFVIIEEKLVNAQQLPSFERFVLQTKEHIK
RITATPIRWFGLQFSEVTIERVPLILSEVSGLAISERNNSRALEEKYTDNL"
gene 18843..19990
/locus_tag="LLKF_RS12915"
/old_locus_tag="LLKF_p0022"
CDS join(18843..19095,19095..19990)
/locus_tag="LLKF_RS12915"
/old_locus_tag="LLKF_p0022"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_086558257.1"
/ribosomal_slippage
/GO_function="GO:0004803 - transposase activity [Evidence
IEA]"
/note="programmed frameshift; Derived by automated
computational analysis using gene prediction method:
Protein Homology."
/codon_start=1
/transl_table=11
/product="IS3 family transposase"
/protein_id="WP_144242131.1"
/translation="MARRKFDKQFKNSAVKLILEEGYSVKEVSQELEVHANSLYRWVQ
EVEEYGESAFPGNGTALADAQHKIKLLEKENRYLQEELELPKKVPCLLEAKQVKRFKF
LLKHHGKIKIKHAVKVLKVYRSGFYEYMHRRPSKQQVEREILSEKIKAVFHEHKGRYG
AVRITKVLHNTGIMTNTKRVGKLMHLMGLYAKGSRYKYKHYNRKGASLSRPNLINQIF
KATAPNKVGLGDMTYIPTKEGTLYLAVNIDVFSRKIVGWSMSSRMQDKLVRDCFLQAC
GKEHPQPGLIVHTDQGSQYTSSRYQSTLRQVGAQSSMSRKGNPYDNAMMESFYKMLKR
ELINDAHFETRAEATQEIFKYIETYYSTKRMHSGLDYKSPKDFEKYNS"
gene 20452..20553
/locus_tag="LLKF_RS13445"
CDS 20452..20553
/locus_tag="LLKF_RS13445"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_000491388.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="type I toxin-antitoxin system Fst family toxin"
/protein_id="WP_021722009.1"
/translation="MLITIFVSVVAPIVVGVILELVKDWLKNRHKRL"
gene complement(20931..21488)
/locus_tag="LLKF_RS12920"
/old_locus_tag="LLKF_p0023"
CDS complement(20931..21488)
/locus_tag="LLKF_RS12920"
/old_locus_tag="LLKF_p0023"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_012304947.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="recombinase family protein"
/protein_id="WP_012898744.1"
/translation="MQQNPRVSTFEQKLESQIEVLKEAGAEEVFQEKFTGTTVERPQF
NLVLKKLENGDTLIVTKLDRLARNTREVLEIVQSLFNRGIKVHILNIGLIDNTPTGQL
IFTIFSAFAQFERDLIVTRTQEGKNFAKIHDPSFKEGRPQKFTEEQIQFAHELKQQGM
TYKMIERKTGISIATQKRRFGAIKK"
gene 21549..22229
/locus_tag="LLKF_RS12925"
/pseudo
CDS 21549..22229
/locus_tag="LLKF_RS12925"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_012477736.1"
/GO_function="GO:0004803 - transposase activity [Evidence
IEA]"
/note="internal stop; Derived by automated computational
analysis using gene prediction method: Protein Homology."
/pseudo
/codon_start=1
/transl_table=11
/product="IS6-like element ISS1N family transposase"
gene complement(22408..23157)
/gene="pnuC"
/locus_tag="LLKF_RS12930"
/old_locus_tag="LLKF_p0026"
CDS complement(22408..23157)
/gene="pnuC"
/locus_tag="LLKF_RS12930"
/old_locus_tag="LLKF_p0026"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_011915260.1"
/GO_component="GO:0016020 - membrane [Evidence IEA]"
/GO_function="GO:0015663 - nicotinamide mononucleotide
transmembrane transporter activity [Evidence IEA]"
/GO_process="GO:0015890 - nicotinamide mononucleotide
transport [Evidence IEA]"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="nicotinamide riboside transporter PnuC"
/protein_id="WP_011915260.1"
/translation="MNKNQFKIFREAIKQTFSIKKIFEELSTLHIQEYLLLFLMTIAQ
VISFLFTKDASVLVLILSISSIINLVLVDRGRITNYFWGSITTTAWVIIAIHTRLIGD
IASQTFYLIMQFLGIYIWGKELRATTKHEVQSKKLTTFQALIYFVGTILLYFMIVIIS
KKLGGVQIWLDAALFPLAIVGQILMTFGYRSQWVAWNIINIINIIIWYNQFMVNGTSI
SLSMLMLQIVMFLNSIYGSIIWLKKSKSYTI"
gene complement(23724..23903)
/locus_tag="LLKF_RS12935"
CDS complement(23724..23903)
/locus_tag="LLKF_RS12935"
/inference="COORDINATES: ab initio
prediction:GeneMarkS-2+"
/note="Derived by automated computational analysis using
gene prediction method: GeneMarkS-2+."
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="WP_041168157.1"
/translation="MKKGKLLLATTLVAGTVLGYCYRDELLKIIPINKKPVPKSEIKN
IPVYIRETKTKGEGL"
gene complement(23915..25684)
/locus_tag="LLKF_RS12940"
/old_locus_tag="LLKF_p0027"
CDS complement(23915..25684)
/locus_tag="LLKF_RS12940"
/old_locus_tag="LLKF_p0027"
/EC_number="4.2.1.53"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_019803487.1"
/GO_function="GO:0050151 - oleate hydratase activity
[Evidence IEA]"
/GO_function="GO:0071949 - FAD binding [Evidence IEA]"
/GO_process="GO:0006631 - fatty acid metabolic process
[Evidence IEA]"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="oleate hydratase"
/protein_id="WP_012898745.1"
/translation="MYYSNGNYEAFAKPKKPKNVENKSAYIVGSGLAGLASAIFLIRD
GQMPGENIHIFEELSLAGGSMDGIYNESRGYIIRGGREMEAHFETLWDLFRSIPTLEN
PEISVLDEFYWLNKEDPSYSKTRVIEKCGKQLENDGELTLSRKAIDEMLKLVLTSESQ
LDDVKINEVFSDEFFESNFWLYWSTMFAFEPWASAMEMRRYLLRFVQHVGTLKDMSAL
KFTKFNQYESLVKPMIHYLEGHNVHFQYDTVVNNIIVNRSDSQKIATEIQLTESGQTK
VLPLTIDDLVIVTNGSITESTTYGDTEHPAPQEHEVGQSWQLWENIAKQDKSFGRPWK
FYRNVPVANWVNSTTLTFSDDQVVPFLESITGKDPHSGSLVTSGPITIRDSNWLLGIS
ISRQPHFEQQKDNELIVWLYGLFSDLPGNYIPKKITDCTGREICEEFLYHIGVPKNKI
SEIAATVKAVPTNMPYITSYFMPRAAGDRPKVVPDGSANLAFVGNFAETERDTVFTTE
YSVRTAMEAVYQLLDVDRGIPEVFDSSFDMRMILNAIYYLNDKKGLRDLPLTKVEKFG
LEAFLKKIKGTYIEEILVDQHLL"
gene 26029..26157
/locus_tag="LLKF_RS13920"
CDS 26029..26157
/locus_tag="LLKF_RS13920"
/inference="COORDINATES: ab initio
prediction:GeneMarkS-2+"
/note="Derived by automated computational analysis using
gene prediction method: GeneMarkS-2+."
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="WP_255596497.1"
/translation="MLKIVGENRKLTLDMRALDDSIYSDIDSDKLNQVVDDISPQY"
gene complement(26452..26988)
/locus_tag="LLKF_RS12945"
/old_locus_tag="LLKF_p0028"
CDS complement(26452..26988)
/locus_tag="LLKF_RS12945"
/old_locus_tag="LLKF_p0028"
/inference="COORDINATES: protein motif:HMM:NF037267.2"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="DUF5626 family protein"
/protein_id="WP_012898746.1"
/translation="MKKSWKKLAISSGIVLLLSGALAPSLIVKADTTEQKSLPVEVQN
DSNLSAEFDLSKLPLNGETKSVTDSQGNVTYFKVESGSNPMARISNGSHKVTAWDAAW
HVSFYITTSGNKITSARDLNYTIVGAQVNSASLRVDNSKRASAHFSFTTPIWNVISWT
GWVRATINSSNNLVVTRN"
gene complement(28448..28729)
/locus_tag="LLKF_RS12950"
/old_locus_tag="LLKF_p0029"
CDS complement(28448..28729)
/locus_tag="LLKF_RS12950"
/old_locus_tag="LLKF_p0029"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_011834682.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="WP_011915250.1"
/translation="MKYFVTALSPSKNMGTMNWQTMILGDYCVNDSYWENAKRELSEE
VQWVTQSDLYKKIKRNHDSNDEIILSKPVSIILETVKSDFPHANVWVYQ"
gene complement(29755..30207)
/locus_tag="LLKF_RS12955"
/old_locus_tag="LLKF_p0030"
CDS complement(29755..30207)
/locus_tag="LLKF_RS12955"
/old_locus_tag="LLKF_p0030"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_017865356.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="GNAT family N-acetyltransferase"
/protein_id="WP_012898747.1"
/translation="MKLRPIETTDFPLIVKKYVDVFAAEPWNETHDPTHINEYIQNIY
AMNTFIGFIAHDDHSNDFIGVALGFIKPWYQGTEYILDTFFIDNTKQHSGLGTIFLKH
IKQELHIRSISAIMLDTDRSTPAENFYTKNGFEALEESVLMVSSTTDV"
gene complement(30699..31304)
/locus_tag="LLKF_RS12960"
/old_locus_tag="LLKF_p0031"
CDS complement(30699..31304)
/locus_tag="LLKF_RS12960"
/old_locus_tag="LLKF_p0031"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_012897399.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="helix-turn-helix transcriptional regulator"
/protein_id="WP_012898748.1"
/translation="MNKYRVAELRKKRGWTQEVLAEKANITVRTIQRIENGTDVSLDT
LASISNALLVPVSELFERIDEEAKEVEIMDMSKEQLIQLKYRQTITVSITLLVIAAIL
LVMSILGVEINELASGYNTTLSWISWVSLLLLLIGLANYYLGVKLNETLDQKYPLTKG
IKLKEKKERFENFWQFFSIYWWMIFPIFGFITWFISFFNSL"
gene <31711..32163
/locus_tag="LLKF_RS13345"
/pseudo
CDS <31711..32163
/locus_tag="LLKF_RS13345"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_076611694.1"
/GO_function="GO:0004803 - transposase activity [Evidence
IEA]"
/note="incomplete; partial in the middle of a contig;
missing N-terminus; Derived by automated computational
analysis using gene prediction method: Protein Homology."
/pseudo
/codon_start=1
/transl_table=11
/product="IS6 family transposase"
gene complement(32220..32909)
/locus_tag="LLKF_RS12965"
/old_locus_tag="LLKF_p0033"
CDS complement(32220..32909)
/locus_tag="LLKF_RS12965"
/old_locus_tag="LLKF_p0033"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_011834689.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="Crp/Fnr family transcriptional regulator"
/protein_id="WP_012898750.1"
/translation="MSKYEHHFSHDEHHCVQLVPLFALLSESELAQVEQVVHHKTFEK
GEAVISPFTVPQLAIVAHGTLKIYQLSSAGKEQLLRVIEPGGYAGEDALFGVVNENLY
GETLQETKICFLRQQDFKNLLLKYPKLSLKLLETTVRRAAEMQYQAQFLMMEDVESRI
ANYLLQLVKVVDSNSVMIPMKMKDLATFIGTTPETISRKFKILEEKGFIERRGKIIKI
LDIDSLEDDYA"
gene complement(32995..33540)
/locus_tag="LLKF_RS12970"
/old_locus_tag="LLKF_p0034"
CDS complement(32995..33540)
/locus_tag="LLKF_RS12970"
/old_locus_tag="LLKF_p0034"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_019291976.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="DNA-binding protein"
/protein_id="WP_011915219.1"
/translation="MTKLTIDEKYAKELDKAEVDHHKPTAGAMLGHVLSNLFIENVRL
TQAGIYAKSPVKCEYLREIAKKEDEYFFKISDLLLDENEIVPSTTEEFLKYHKFITED
PKAKYWTDEALLESFIADFQAQNMFITRAIKLANKEEKFALAAVVVELYGYNLQVIRH
LAGDLGKAVADFHDEDEDNDN"
gene complement(33589..33816)
/locus_tag="LLKF_RS12975"
/old_locus_tag="LLKF_p0035"
CDS complement(33589..33816)
/locus_tag="LLKF_RS12975"
/old_locus_tag="LLKF_p0035"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_019335409.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="cation transporter"
/protein_id="WP_012898751.1"
/translation="MSKVIMKLNELSCPSCMAKIEGAMTTTKGVANAKVLFNASKVKA
EFDENVVSADELISKVEKLGYPVLSSKVTVV"
gene complement(34523..36385)
/locus_tag="LLKF_RS12980"
/old_locus_tag="LLKF_p0036"
CDS complement(34523..36385)
/locus_tag="LLKF_RS12980"
/old_locus_tag="LLKF_p0036"
/EC_number="7.2.2.-"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_012898752.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="heavy metal translocating P-type ATPase"
/protein_id="WP_012898752.1"
/translation="MAKITLDFQKRLQQHSNHLVILSAILIVLGYLGKYGVNQIWIWN
STMIIASIIGFIPVAIHAYQAIKVKQISIDLLVSIAVIGALFIGEYEESAIVTFLFAF
GGFLEKKTLEKTRSSIKELTNMAPSTALSADGEEMGIDEVEIGDKLLVKTGRQIPVDG
RIYQGSGYVNEASITGESREIRKEAGTKVFAGSILENGTIYVEAEKVGEDTTFGKIIE
LVEEAQDTKSPAEKFIDRFAKYYTPAVLVIAAITWVFSHNLELAITILVLGCPGALVI
GAPVSNVAGIGNGAKRGVLIKGGDVMNTFSHIDTLLFDKTGTLTKGNTEVVVVKNYGA
SKELIDAVASAENESDHPLATAVVRMIGKFNPIKFEKTDVVKGQGIIADDLLIGNEKM
MVVNHITISPEQKQDITEITDSGASVVLVAADNRLQLIYGIADEIRSGVKESLEELRH
EGISRMIMLTGDNETTAKAVAAQLGIDEVRANLMPEEKAEVVKSLKNSGKKIAFIGDG
VNDSPSLALANIGIAMGSGTDTAIETSDIVLMKSSFDELVHAYGLSKRTVANMTQNIV
IAIVVVLFLLASLILGGTGLVPSFVNMGTGMFVHEASILIVIVNGMRLIRYREK"
gene complement(36680..37303)
/locus_tag="LLKF_RS12985"
/pseudo
CDS complement(36680..37303)
/locus_tag="LLKF_RS12985"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_012477736.1"
/GO_function="GO:0004803 - transposase activity [Evidence
IEA]"
/note="internal stop; Derived by automated computational
analysis using gene prediction method: Protein Homology."
/pseudo
/codon_start=1
/transl_table=11
/product="IS6 family transposase"
CONTIG join(CP001835.1:1..37510)
//