LOCUS       XP_002490737             720 aa            linear   PLN 22-JUL-2009
DEFINITION  Glutamyl-tRNA synthetase, cytoplasmic [Komagataella pastoris
            GS115].
ACCESSION   XP_002490737
VERSION     XP_002490737.1  GI:254567253
DBSOURCE    REFSEQ: accession XM_002490692.1
KEYWORDS    .
SOURCE      Komagataella pastoris GS115 (Pichia pastoris GS115)
  ORGANISM  Komagataella pastoris GS115
            Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
            Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
            Komagataella.
REFERENCE   1
  AUTHORS   De Schutter,K., Lin,Y.C., Tiels,P., Van Hecke,A., Glinka,S.,
            Weber-Lehmann,J., Rouze,P., Van de Peer,Y. and Callewaert,N.
  TITLE     Genome sequence of the recombinant protein production host Pichia
            pastoris
  JOURNAL   Nat. Biotechnol. 27 (6), 561-566 (2009)
   PUBMED   19465926
REFERENCE   2  (residues 1 to 720)
  AUTHORS   Callewaert,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2009) Callewaert N., Department for Molecular
            Biomedical Research, Flanders Institute for Biotechnology (VIB) /
            Gent University, Technologiepark 927, Ghent, 9052, BELGIUM
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CAY68457.
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Komagataella pastoris GS115"
                     /strain="GS115"
                     /db_xref="taxon:644223"
                     /chromosome="1"
     Protein         1..720
                     /product="Glutamyl-tRNA synthetase, cytoplasmic"
                     /calculated_mol_wt=82223
     Region          47..711
                     /region_name="PLN02907"
                     /note="glutamate-tRNA ligase"
                     /db_xref="CDD:178495"
     Region          79..162
                     /region_name="GST_C_GluRS_N"
                     /note="Glutathione S-transferase C-terminal-like, alpha
                     helical domain of Glutamyl-tRNA synthetase; cd10306"
                     /db_xref="CDD:198339"
     Site            order(79..81,83..88,90..91,104,107,113..114)
                     /site_type="other"
                     /note="putative protein interface 1 [polypeptide binding]"
                     /db_xref="CDD:198339"
     Site            order(113..116,147,150..151,154,158,161..162)
                     /site_type="other"
                     /note="Arc1p interface [polypeptide binding]"
                     /db_xref="CDD:198339"
     Region          210..>303
                     /region_name="nt_trans"
                     /note="nucleotidyl transferase superfamily; cl00015"
                     /db_xref="CDD:212170"
     Site            224..227
                     /site_type="active"
                     /db_xref="CDD:173912"
     Site            224..227
                     /site_type="other"
                     /note="HIGH motif"
                     /db_xref="CDD:173912"
     Site            order(224,227)
                     /site_type="other"
                     /note="nucleotide binding site [chemical binding]"
                     /db_xref="CDD:173912"
     Region          <378..520
                     /region_name="nt_trans"
                     /note="nucleotidyl transferase superfamily; cl00015"
                     /db_xref="CDD:212170"
     Site            448..451
                     /site_type="active"
                     /db_xref="CDD:173912"
     Site            448..451
                     /site_type="other"
                     /note="KMSKS motif"
                     /db_xref="CDD:173912"
     Region          518..695
                     /region_name="tRNA-synt_1c_C"
                     /note="tRNA synthetases class I (E and Q), anti-codon
                     binding domain; pfam03950"
                     /db_xref="CDD:202825"
     CDS             1..720
                     /locus_tag="PAS_chr1-4_0598"
                     /coded_by="XM_002490692.1:1..2163"
                     /db_xref="GeneID:8197728"
ORIGIN      
        1 mttlslaaka plidyaslva safvntgtdn qitldfqdvk elekditvsv skdekvvasn
       61 idnvlnffae eypqviigke eaqewikfak deltvkdfkk lsvslekles hlnfrsfivg
      121 fkfslsdiav wgvlranplm gsviknsvyi nvtrwyqfle sdkrfgavve lmtksiaeir
      181 klakqqstgg kkeghkanfd idlpgakige vvtrfppeps gylhighaka avlnkyfadm
      241 ykgkliirfd dtnpskekee fqdsiledlk llgikgdkit yssdyfqimy dyavqmikdg
      301 kaycddtpse qmraermdgi kssrrdrtve enlqifteem kngteiglkn clrakidyen
      361 pnkalrdpai yrcnltphhr tgttwkmypl ydfcvpivds legvthalrt neyrdrnpqy
      421 qwmlealglr kvhiwdfarv nfirtllskr klqwfvdkgy vgnwddprfp tvrgvrrrgm
      481 tvegltnfii sqgpskniin ldwnliwavn kkiidpiapr htalnkeniv pvkltgapss
      541 aviedklkhk knpevgykkv ifidnvlveq ddaatfeege eitfmdwgnv ivdkihknda
      601 givtsidatl hlegdfkktk kkinwlantd dkvevelvdf dhlitkdkle egdnfedfit
      661 pttefhsiav adlnvkdmkv gdiiqferkg yyrldsdkdg kyvfftipdg ksvnrygakk
//