KEGG   REACTION: R08700Help
Entry
R08700                      Reaction                               

Name
[enzyme]-S-sulfanylcysteine:tRNA-uridine sulfurtransferase (ATP-hydrolysing)
Definition
tRNA uridine + [Enzyme]-S-sulfanylcysteine + ATP <=> tRNA containing 2-thiouridine + [Enzyme]-cysteine + AMP + Diphosphate
Equation
Comment
multi-step reaction
incomplete reaction (donor is unidentified in vivo, dithiothreitol[CPD:C00265] in vitro)
RPair
C00002_C00020 ligase
C00002_C00013 ligase
C15811_C15812 main [RC:RC02313]
C15812_C17322 trans
C00868_C17322 main [RC:RC02315]
Enzyme
Reference
1  [PMID:12549933]
Kambampati R, Lauhon CT.
MnmA and IscS are required for in vitro 2-thiouridine biosynthesis in Escherichia coli.
Biochemistry. 42 (2003) 1109-17.

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