ID AMPA_RHIL3 Reviewed; 496 AA.
AC Q1MIZ4;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2006, sequence version 1.
DT 29-MAY-2013, entry version 53.
DE RecName: Full=Probable cytosol aminopeptidase;
DE EC=3.4.11.1;
DE AltName: Full=Leucine aminopeptidase;
DE Short=LAP;
DE EC=3.4.11.10;
DE AltName: Full=Leucyl aminopeptidase;
GN Name=pepA; OrderedLocusNames=RL1571;
OS Rhizobium leguminosarum bv. viciae (strain 3841).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX NCBI_TaxID=216596;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3841;
RX PubMed=16640791; DOI=10.1186/gb-2006-7-4-r34;
RA Young J.P.W., Crossman L.C., Johnston A.W.B., Thomson N.R.,
RA Ghazoui Z.F., Hull K.H., Wexler M., Curson A.R.J., Todd J.D.,
RA Poole P.S., Mauchline T.H., East A.K., Quail M.A., Churcher C.,
RA Arrowsmith C., Cherevach I., Chillingworth T., Clarke K., Cronin A.,
RA Davis P., Fraser A., Hance Z., Hauser H., Jagels K., Moule S.,
RA Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M.,
RA Whitehead S., Parkhill J.;
RT "The genome of Rhizobium leguminosarum has recognizable core and
RT accessory components.";
RL Genome Biol. 7:R34.1-R34.20(2006).
CC -!- FUNCTION: Presumably involved in the processing and regular
CC turnover of intracellular proteins. Catalyzes the removal of
CC unsubstituted N-terminal amino acids from various peptides (By
CC similarity).
CC -!- CATALYTIC ACTIVITY: Release of an N-terminal amino acid, Xaa-|-
CC Yaa-, in which Xaa is preferably Leu, but may be other amino acids
CC including Pro although not Arg or Lys, and Yaa may be Pro. Amino
CC acid amides and methyl esters are also readily hydrolyzed, but
CC rates on arylamides are exceedingly low.
CC -!- CATALYTIC ACTIVITY: Release of an N-terminal amino acid,
CC preferentially leucine, but not glutamic or aspartic acids.
CC -!- COFACTOR: Binds 2 manganese ions per subunit (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Belongs to the peptidase M17 family.
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DR EMBL; AM236080; CAK07066.1; -; Genomic_DNA.
DR RefSeq; YP_767175.1; NC_008380.1.
DR ProteinModelPortal; Q1MIZ4; -.
DR STRING; 216596.RL1571; -.
DR MEROPS; M17.003; -.
DR EnsemblBacteria; CAK07066; CAK07066; RL1571.
DR GeneID; 4401650; -.
DR KEGG; rle:RL1571; -.
DR PATRIC; 23139675; VBIRhiLeg32091_2779.
DR eggNOG; COG0260; -.
DR HOGENOM; HOG000243129; -.
DR KO; K01255; -.
DR OMA; HAWVKNK; -.
DR ProtClustDB; PRK00913; -.
DR BioCyc; RLEG216596:GKE5-1603-MONOMER; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004177; F:aminopeptidase activity; IEA:HAMAP.
DR GO; GO:0030145; F:manganese ion binding; IEA:HAMAP.
DR GO; GO:0008235; F:metalloexopeptidase activity; IEA:HAMAP.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR HAMAP; MF_00181; Cytosol_peptidase_M17; 1; -.
DR InterPro; IPR011356; Leucine_aapep/pepB.
DR InterPro; IPR000819; Peptidase_M17_C.
DR InterPro; IPR023042; Peptidase_M17_leu_NH2_pept.
DR InterPro; IPR008283; Peptidase_M17_N.
DR PANTHER; PTHR11963:SF3; PTHR11963:SF3; 1.
DR Pfam; PF00883; Peptidase_M17; 1.
DR Pfam; PF02789; Peptidase_M17_N; 1.
DR PRINTS; PR00481; LAMNOPPTDASE.
DR PROSITE; PS00631; CYTOSOL_AP; 1.
PE 3: Inferred from homology;
KW Aminopeptidase; Complete proteome; Cytoplasm; Hydrolase; Manganese;
KW Metal-binding; Protease.
FT CHAIN 1 496 Probable cytosol aminopeptidase.
FT /FTId=PRO_1000019966.
FT ACT_SITE 274 274 Potential.
FT ACT_SITE 348 348 Potential.
FT METAL 262 262 Manganese 2 (By similarity).
FT METAL 267 267 Manganese 1 (By similarity).
FT METAL 267 267 Manganese 2 (By similarity).
FT METAL 285 285 Manganese 2 (By similarity).
FT METAL 344 344 Manganese 1 (By similarity).
FT METAL 346 346 Manganese 1 (By similarity).
FT METAL 346 346 Manganese 2 (By similarity).
SQ SEQUENCE 496 AA; 52209 MW; 76271929591BBD3D CRC64;
MSIKLEISFS KSAKLNGGLA ILLKTAEADS AAGAETVDPA GVIVKAARIA RYSAKSMNGL
DIVVPEGAPV DRIVVIGLGK AAELTAHDWL KAGGAAASRI KNTDKAAVFI DVPGLTTSPR
AAADFALGML LRAYSFDTYK TKKGDEEEKP AKSVKVTIVT ADPAGAKKAF SDSEAIAGGV
NLARDLVNEP PNVLGPVEFA AKAKELEKLG VEVEILTERE MRRLGMGALL GVAQGSVRPP
RLAVMQWKGG KGKDRPVAFI GKGVVFDTGG ISIKPAAGME DMKGDMGGAA AVTGLMHVLA
SRKAAVNAVG IIGLVENMPD GNAQRPGDIV TSMSGQTIEV INTDAEGRLV LCDALWYCND
RFKPQFMINL ATLTGAIIVA LGNVHAGLFS NDDQLSAQLT AAGLSSNEKL WRMPLGRDYD
KLIDSKFADM KNTGGRQAGS ITAAHFLKRF VQDTPWAHLD IAGTAMGSPQ DEINQSWGSG
FGVRLLDELV RAHYES
//
