ID DAPE_PARL1 Reviewed; 395 AA.
AC A7HPQ6;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 1.
DT 01-MAY-2013, entry version 41.
DE RecName: Full=Succinyl-diaminopimelate desuccinylase;
DE Short=SDAP desuccinylase;
DE EC=3.5.1.18;
DE AltName: Full=N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase;
GN Name=dapE; OrderedLocusNames=Plav_0266;
OS Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC Phyllobacteriaceae; Parvibaculum.
OX NCBI_TaxID=402881;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DS-1 / DSM 13023 / NCIMB 13966;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T.,
RA Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D.,
RA Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L.,
RA Kyrpides N., Mikhailova N., Richardson P.;
RT "Complete genome sequence and annotation of Parvibaculum
RT lavamentivorans DS-1.";
RL Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the hydrolysis of N-succinyl-L,L-
CC diaminopimelic acid (SDAP), forming succinate and LL-2,6-
CC diaminoheptanedioate (DAP), an intermediate involved in the
CC bacterial biosynthesis of lysine and meso-diaminopimelic acid, an
CC essential component of bacterial cell walls (By similarity).
CC -!- CATALYTIC ACTIVITY: N-succinyl-LL-2,6-diaminoheptanedioate + H(2)O
CC = succinate + LL-2,6-diaminoheptanedioate.
CC -!- COFACTOR: Binds 1 Zn(2+) ion per subunit (By similarity).
CC -!- COFACTOR: Binds 1 Co(2+) ion per subunit (By similarity).
CC -!- PATHWAY: Amino-acid biosynthesis; L-lysine biosynthesis via DAP
CC pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate
CC (succinylase route): step 3/3.
CC -!- SUBUNIT: Homodimer (By similarity).
CC -!- SIMILARITY: Belongs to the peptidase M20A family. DapE subfamily.
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DR EMBL; CP000774; ABS61889.1; -; Genomic_DNA.
DR RefSeq; YP_001411546.1; NC_009719.1.
DR ProteinModelPortal; A7HPQ6; -.
DR STRING; 402881.Plav_0266; -.
DR EnsemblBacteria; ABS61889; ABS61889; Plav_0266.
DR GeneID; 5454257; -.
DR KEGG; pla:Plav_0266; -.
DR PATRIC; 22862062; VBIParLav90819_0279.
DR eggNOG; COG0624; -.
DR HOGENOM; HOG000243770; -.
DR KO; K01439; -.
DR OMA; WDAPRLE; -.
DR ProtClustDB; PRK13009; -.
DR BioCyc; PLAV402881:GHQA-316-MONOMER; -.
DR UniPathway; UPA00034; UER00021.
DR GO; GO:0050897; F:cobalt ion binding; IEA:HAMAP.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:InterPro.
DR GO; GO:0009014; F:succinyl-diaminopimelate desuccinylase activity; IEA:HAMAP.
DR GO; GO:0008270; F:zinc ion binding; IEA:HAMAP.
DR GO; GO:0019877; P:diaminopimelate biosynthetic process; IEA:HAMAP.
DR GO; GO:0009089; P:lysine biosynthetic process via diaminopimelate; IEA:HAMAP.
DR GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR HAMAP; MF_01690; DapE; 1; -.
DR InterPro; IPR001261; ArgE/DapE_CS.
DR InterPro; IPR005941; DapE_proteobac.
DR InterPro; IPR002933; Peptidase_M20.
DR InterPro; IPR011650; Peptidase_M20_dimer.
DR Pfam; PF07687; M20_dimer; 1.
DR Pfam; PF01546; Peptidase_M20; 1.
DR SUPFAM; SSF55031; Peptidase_M20_dimer; 1.
DR TIGRFAMs; TIGR01246; dapE_proteo; 1.
DR PROSITE; PS00758; ARGE_DAPE_CPG2_1; FALSE_NEG.
DR PROSITE; PS00759; ARGE_DAPE_CPG2_2; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Cobalt; Complete proteome;
KW Diaminopimelate biosynthesis; Hydrolase; Lysine biosynthesis;
KW Metal-binding; Zinc.
FT CHAIN 1 395 Succinyl-diaminopimelate desuccinylase.
FT /FTId=PRO_0000375640.
FT ACT_SITE 83 83 By similarity.
FT ACT_SITE 146 146 Proton acceptor (By similarity).
FT METAL 81 81 Cobalt or zinc 1 (By similarity).
FT METAL 114 114 Cobalt or zinc 1 (By similarity).
FT METAL 114 114 Cobalt or zinc 2 (By similarity).
FT METAL 147 147 Cobalt or zinc 2 (By similarity).
FT METAL 175 175 Cobalt or zinc 1 (By similarity).
FT METAL 364 364 Cobalt or zinc 2 (By similarity).
SQ SEQUENCE 395 AA; 42041 MW; 4797D7D944CD5EC0 CRC64;
MTAPASSPAS PYDPLDIAVE LIRCPSVTPD EGGALGVLEK WLAPLGFKCE RMRFSAEGTP
DVDNLYARLG SGHPHFCFAG HTDVVPVGQA DAWSVDPFAA DIKDGRLYGR GAADMKSAVA
SFVAAAERIS REGFQGSISL LITGDEEGPS INGTRKMLEK LAARNETIDH CIVGEPTSVE
KLGDMIKVGR RGSINGWLTV QGTQGHVAYP HLADNPVPRL LEMLRRLDAH VLDEGTDHFQ
PSNLEVTTVD IGNTATNVIP GSARATVNIR FNDLHTGASL DKWMRGVLDA VTAEMGGSYS
FKTSVSGEAF ITEPGAFSAL IAEAAKEVTG ITPELSTTGG TSDARFIRAY APVVEIGLPN
ATMHKADENT GVSEIRQLAD IYETVLRGYF AGRAS
//
