ID DNLI_PYRAE Reviewed; 584 AA.
AC O93723;
DT 20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2001, sequence version 2.
DT 01-MAY-2013, entry version 92.
DE RecName: Full=DNA ligase;
DE EC=6.5.1.1;
DE AltName: Full=Polydeoxyribonucleotide synthase [ATP];
GN Name=lig; OrderedLocusNames=PAE0833;
OS Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630
OS / NBRC 100827).
OC Archaea; Crenarchaeota; Thermoprotei; Thermoproteales;
OC Thermoproteaceae; Pyrobaculum.
OX NCBI_TaxID=178306;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827;
RA Baikalov C.J., Slupska M.M., Miller J.H.;
RL Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827;
RX PubMed=11792869; DOI=10.1073/pnas.241636498;
RA Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I.,
RA Miller J.H.;
RT "Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum
RT aerophilum.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002).
CC -!- FUNCTION: DNA ligase that seals nicks in double-stranded DNA
CC during DNA replication, DNA recombination and DNA repair (By
CC similarity).
CC -!- CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) +
CC (deoxyribonucleotide)(m) = AMP + diphosphate +
CC (deoxyribonucleotide)(n+m).
CC -!- COFACTOR: Divalent metal cations (By similarity).
CC -!- SUBUNIT: Monomer (By similarity).
CC -!- SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD00532.1; Type=Erroneous initiation;
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; U82370; AAD00532.1; ALT_INIT; Genomic_DNA.
DR EMBL; AE009441; AAL63064.1; -; Genomic_DNA.
DR RefSeq; NP_558882.1; NC_003364.1.
DR ProteinModelPortal; O93723; -.
DR STRING; 178306.PAE0833; -.
DR EnsemblBacteria; AAL63064; AAL63064; PAE0833.
DR GeneID; 1465293; -.
DR KEGG; pai:PAE0833; -.
DR eggNOG; COG1793; -.
DR HOGENOM; HOG000036008; -.
DR KO; K10747; -.
DR OMA; AQVACLY; -.
DR ProtClustDB; PRK01109; -.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003910; F:DNA ligase (ATP) activity; IEA:HAMAP.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0051103; P:DNA ligation involved in DNA repair; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:HAMAP.
DR GO; GO:0006260; P:DNA replication; IEA:HAMAP.
DR Gene3D; 1.10.3260.10; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR HAMAP; MF_00407; DNA_ligase; 1; -.
DR InterPro; IPR022865; DNA_ligae_ATP-dep_bac/arc.
DR InterPro; IPR000977; DNA_ligase_ATP-dep.
DR InterPro; IPR012309; DNA_ligase_ATP-dep_C.
DR InterPro; IPR012310; DNA_ligase_ATP-dep_cent.
DR InterPro; IPR016059; DNA_ligase_ATP-dep_CS.
DR InterPro; IPR012308; DNA_ligase_ATP-dep_N.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR Pfam; PF04679; DNA_ligase_A_C; 1.
DR Pfam; PF01068; DNA_ligase_A_M; 1.
DR Pfam; PF04675; DNA_ligase_A_N; 1.
DR SUPFAM; SSF50249; Nucleic_acid_OB; 1.
DR SUPFAM; SSF117018; SSF117018; 1.
DR TIGRFAMs; TIGR00574; dnl1; 1.
DR PROSITE; PS00697; DNA_LIGASE_A1; 1.
DR PROSITE; PS00333; DNA_LIGASE_A2; FALSE_NEG.
DR PROSITE; PS50160; DNA_LIGASE_A3; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell cycle; Cell division; Complete proteome; DNA damage;
KW DNA recombination; DNA repair; DNA replication; Ligase; Metal-binding;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1 584 DNA ligase.
FT /FTId=PRO_0000059611.
FT ACT_SITE 251 251 N6-AMP-lysine intermediate (By
FT similarity).
FT BINDING 249 249 ATP (By similarity).
FT BINDING 256 256 ATP (By similarity).
FT BINDING 271 271 ATP (By similarity).
FT BINDING 301 301 ATP (By similarity).
FT BINDING 341 341 ATP (By similarity).
FT BINDING 416 416 ATP (By similarity).
FT BINDING 422 422 ATP (By similarity).
SQ SEQUENCE 584 AA; 64568 MW; 7CB44E8A5B0DBA0A CRC64;
MQFGELVKAL AAIEATTQRT TMVKLLVSLF KKASPEEVGK IVYFILGDLR PPWEGVELGV
AEKLCLRAVS KATGAAVSEL EALYKKTGDV GEAARRALAA SKRPGLLAFG SQKPLEVSEV
YDTLLKVARA TGEGAQDMKV ALLSSLFARA TPEEGKYIAR FVVGKLRLGV ADMTIIEALS
DAYGVNKEAL EKAYHIYPDL GRLAKHVAEG KPLDEIRITP GVPVLPMLAQ RLSSASEILA
KLGGSAICEY KYDGERAQIH LTPGGVKIFS RRLEDITHAY PDVVKAVKEA VSAKEAILEG
EIVAVDPDTG DMLPFQELMH RKRKHDVATA VELYPTVLYL FDVLYVDGED LTEEPLIYRR
VRLSEIVCET DKVSIAKWRI FDNAEAVDVF FHESVSMGTE GLICKSPSSI YEMGARGWNW
IKYKRDYRSE MIDTVDLVVV GAFHGRGKRA GLYGAFLLAA YDPSTDMFYT VCKVGSGFTD
ADLKKMYEIL QPFKIPHRHP RVVSKMEADV WFVPQVVIEV IGAEITLSPL HTCCLGAVRP
GVGLAVRFPR FTGRYRSDKS PEQATTVAEM LELYKRQKKV VQPE
//
