GenomeNet

Database: UniProt/SWISS-PROT
Entry: DPO3B_STAES
LinkDB: DPO3B_STAES
Original site: DPO3B_STAES 
ID   DPO3B_STAES             Reviewed;         377 AA.
AC   Q8CQK6;
DT   28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   30-AUG-2017, entry version 87.
DE   RecName: Full=Beta sliding clamp;
DE            Short=Beta clamp;
DE            Short=Sliding clamp;
DE   AltName: Full=Beta-clamp processivity factor;
DE   AltName: Full=DNA polymerase III beta sliding clamp subunit;
DE   AltName: Full=DNA polymerase III subunit beta;
GN   Name=dnaN; OrderedLocusNames=SE_0002;
OS   Staphylococcus epidermidis (strain ATCC 12228).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=176280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 12228;
RX   PubMed=12950922; DOI=10.1046/j.1365-2958.2003.03671.x;
RA   Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J.,
RA   Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z.,
RA   Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M.;
RT   "Genome-based analysis of virulence genes in a non-biofilm-forming
RT   Staphylococcus epidermidis strain (ATCC 12228).";
RL   Mol. Microbiol. 49:1577-1593(2003).
CC   -!- FUNCTION: Confers DNA tethering and processivity to DNA
CC       polymerases and other proteins. Acts as a clamp, forming a ring
CC       around DNA (a reaction catalyzed by the clamp-loading complex)
CC       which diffuses in an ATP-independent manner freely and
CC       bidirectionally along dsDNA. Initially characterized for its
CC       ability to contact the catalytic subunit of DNA polymerase III
CC       (Pol III), a complex, multichain enzyme responsible for most of
CC       the replicative synthesis in bacteria; Pol III exhibits 3'-5'
CC       exonuclease proofreading activity. The beta chain is required for
CC       initiation of replication as well as for processivity of DNA
CC       replication. {ECO:0000250|UniProtKB:P0A988}.
CC   -!- SUBUNIT: Forms a ring-shaped head-to-tail homodimer around DNA
CC       which binds and tethers DNA polymerases and other proteins to the
CC       DNA. The DNA replisome complex has a single clamp-loading complex
CC       (3 tau and 1 each of delta, delta', psi and chi subunits) which
CC       binds 3 Pol III cores (1 core on the leading strand and 2 on the
CC       lagging strand) each with a beta sliding clamp dimer. Additional
CC       proteins in the replisome are other copies of gamma, psi and chi,
CC       Ssb, DNA helicase and RNA primase. {ECO:0000250|UniProtKB:P0A988}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P0A988}.
CC   -!- SIMILARITY: Belongs to the beta sliding clamp family.
CC       {ECO:0000305}.
DR   EMBL; AE015929; AAO03599.1; -; Genomic_DNA.
DR   RefSeq; NP_763557.1; NC_004461.1.
DR   RefSeq; WP_001831813.1; NC_004461.1.
DR   ProteinModelPortal; Q8CQK6; -.
DR   SMR; Q8CQK6; -.
DR   STRING; 176280.SE0002; -.
DR   PRIDE; Q8CQK6; -.
DR   EnsemblBacteria; AAO03599; AAO03599; SE_0002.
DR   GeneID; 1056819; -.
DR   KEGG; sep:SE0002; -.
DR   PATRIC; fig|176280.10.peg.2; -.
DR   eggNOG; ENOG4105CZ8; Bacteria.
DR   eggNOG; COG0592; LUCA.
DR   KO; K02338; -.
DR   OMA; NYEAVIP; -.
DR   Proteomes; UP000001411; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0009360; C:DNA polymerase III complex; IEA:InterPro.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   CDD; cd00140; beta_clamp; 1.
DR   InterPro; IPR001001; DNA_polIII_beta.
DR   InterPro; IPR022635; DNA_polIII_beta_C.
DR   InterPro; IPR022637; DNA_polIII_beta_cen.
DR   InterPro; IPR022634; DNA_polIII_beta_N.
DR   PANTHER; PTHR30478:SF1; PTHR30478:SF1; 1.
DR   Pfam; PF00712; DNA_pol3_beta; 1.
DR   Pfam; PF02767; DNA_pol3_beta_2; 1.
DR   Pfam; PF02768; DNA_pol3_beta_3; 1.
DR   PIRSF; PIRSF000804; DNA_pol_III_b; 1.
DR   SMART; SM00480; POL3Bc; 1.
DR   TIGRFAMs; TIGR00663; dnan; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; DNA replication; DNA-binding;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Transferase.
FT   CHAIN         1    377       Beta sliding clamp.
FT                                /FTId=PRO_0000105469.
SQ   SEQUENCE   377 AA;  42102 MW;  4188F2AFFD3BCBDD CRC64;
     MMEFTIKRDY FINQLNDTLK AISPRTTLPI LTGIKIDAKE NEVILTGSDS EISIEITIPK
     QVDGEEIVEI TETGSVVLPG RFFVDIIKKL PGKEVKLSTN EQFQTLITSG HSEFNLSGLD
     PDQYPLLPEV SRDDAIQLSV KVLKNIIAQT NFAVSTSETR PVLTGVNWLI QDNELICTAT
     DSHRLAVRKL QLEDESENKN VIIPGKALSE LNKIMSDSDE DIDIFFASNQ VLFRVGNINF
     ISRLLEGHYP DTTRLFPENY EIKLGINNGD FYHAIDRASL LAREGGNNVI KLSTGNELVE
     LSSTSPEIGT VKEEVNANDV EGGNLKISFN SKYMMDALKA IDNDEVEVEF FGTMKPFILK
     PKDDDSVTQL ILPIRTY
//
DBGET integrated database retrieval system