ID GATB_PSEPF Reviewed; 481 AA.
AC Q3KI28;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 01-MAY-2013, entry version 58.
DE RecName: Full=Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B;
DE Short=Asp/Glu-ADT subunit B;
DE EC=6.3.5.-;
GN Name=gatB; OrderedLocusNames=Pfl01_0835;
OS Pseudomonas fluorescens (strain Pf0-1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=205922;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Pf0-1;
RX PubMed=19432983; DOI=10.1186/gb-2009-10-5-r51;
RA Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R.,
RA Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M.,
RA Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J.,
RA Harris S., Challis G.L., Yaxley A.M., Harris D., Seeger K., Murphy L.,
RA Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K.,
RA Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M.,
RA Parkhill J., Levy S.B., Rainey P.B., Thomson N.R.;
RT "Genomic and genetic analyses of diversity and plant interactions of
RT Pseudomonas fluorescens.";
RL Genome Biol. 10:R51.1-R51.16(2009).
CC -!- FUNCTION: Allows the formation of correctly charged Asn-tRNA(Asn)
CC or Gln-tRNA(Gln) through the transamidation of misacylated Asp-
CC tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both
CC of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction
CC takes place in the presence of glutamine and ATP through an
CC activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) (By
CC similarity).
CC -!- CATALYTIC ACTIVITY: ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP
CC + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.
CC -!- CATALYTIC ACTIVITY: ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP
CC + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate.
CC -!- SUBUNIT: Heterotrimer of A, B and C subunits (By similarity).
CC -!- SIMILARITY: Belongs to the GatB/GatE family. GatB subfamily.
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DR EMBL; CP000094; ABA72578.1; -; Genomic_DNA.
DR RefSeq; YP_346567.1; NC_007492.2.
DR ProteinModelPortal; Q3KI28; -.
DR STRING; 205922.Pfl01_0835; -.
DR World-2DPAGE; 0008:Q3KI28; -.
DR EnsemblBacteria; ABA72578; ABA72578; Pfl01_0835.
DR GeneID; 3715491; -.
DR KEGG; pfo:Pfl01_0835; -.
DR PATRIC; 19883741; VBIPseFlu44242_0847.
DR eggNOG; COG0064; -.
DR HOGENOM; HOG000223742; -.
DR KO; K02434; -.
DR OMA; KNYFYAD; -.
DR ProtClustDB; PRK05477; -.
DR BioCyc; PFLU205922:GJBD-853-MONOMER; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0050567; F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity; IEA:HAMAP.
DR GO; GO:0006412; P:translation; IEA:HAMAP.
DR HAMAP; MF_00121; GatB; 1; -.
DR InterPro; IPR004413; Apn/Gln-ADT_bsu.
DR InterPro; IPR017959; Asn/Gln-tRNA_amidoTrfase_suB/E.
DR InterPro; IPR006075; Asn/Gln-tRNA_Trfase_suB/E_cat.
DR InterPro; IPR018027; Asn/Gln_amidotransferase.
DR InterPro; IPR003789; Asn/Gln_tRNA_amidoTrfrase-rel.
DR InterPro; IPR017958; Gln-tRNA_amidoTrfase_suB_CS.
DR PANTHER; PTHR11659; PTHR11659; 1.
DR Pfam; PF02934; GatB_N; 1.
DR Pfam; PF02637; GatB_Yqey; 1.
DR SMART; SM00845; GatB_Yqey; 1.
DR SUPFAM; SSF89095; GatB_Yqey; 1.
DR TIGRFAMs; TIGR00133; gatB; 1.
DR PROSITE; PS01234; GATB; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Complete proteome; Ligase; Nucleotide-binding;
KW Protein biosynthesis.
FT CHAIN 1 481 Aspartyl/glutamyl-tRNA(Asn/Gln)
FT amidotransferase subunit B.
FT /FTId=PRO_0000241258.
SQ SEQUENCE 481 AA; 52852 MW; 409123E089EB15AF CRC64;
MQWEVVIGLE IHTQLTTRSK IFSGSSTQFG SEPNTQASLI DLGMPGVLPV LNQEAVRMAV
MFGLAIDAEI GQHNVFARKN YFYPDLPKGY QISQMELPIV GKGHLDIALE DGTVKRVGIT
RAHLEEDAGK SLHEEFSGAT GIDLNRAGTP LLEIVSEPDM RSAKEAVAYV KAIHALVRYL
GICDGNMAEG SLRCDCNVSI RPKGQAEFGT RCEIKNVNSF RFIEKAINSE IQRQIDLIED
GGKVIQQTRL YDPNKDETRP MRSKEEANDY RYFPDPDLLP VVIEDSFLND VRATLPELPP
QKRERFQSAF GLSAYDANVL ATSREQADYF EKVASIGGDA KLAANWVMVE LGSLLNKQNL
DIEDSPVSAE QLGGMLQRIK DNTISGKIAK VVFEAMANGE GSADEIIEKC GLKQVTDTGA
ISAVLDEMLA ANAEQVEQYR AADEAKRGKM FGFFVGQAMK ASKGKANPQQ VNELLKSKLE
G
//
