ID ISPE_AZOSB Reviewed; 282 AA.
AC A1K3G8;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 01-MAY-2013, entry version 45.
DE RecName: Full=4-diphosphocytidyl-2-C-methyl-D-erythritol kinase;
DE Short=CMK;
DE EC=2.7.1.148;
DE AltName: Full=4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase;
GN Name=ispE; OrderedLocusNames=azo0756;
OS Azoarcus sp. (strain BH72).
OC Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales;
OC Rhodocyclaceae; Azoarcus.
OX NCBI_TaxID=62928;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BH72;
RX PubMed=17057704; DOI=10.1038/nbt1243;
RA Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J.,
RA Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C.,
RA Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J.,
RA Weidner S., Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A.;
RT "Complete genome of the mutualistic, N2-fixing grass endophyte
RT Azoarcus sp. strain BH72.";
RL Nat. Biotechnol. 24:1385-1391(2006).
CC -!- FUNCTION: Catalyzes the phosphorylation of the position 2 hydroxy
CC group of 4-diphosphocytidyl-2C-methyl-D-erythritol (By
CC similarity).
CC -!- CATALYTIC ACTIVITY: ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-
CC erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-
CC D-erythritol.
CC -!- PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate
CC biosynthesis via DXP pathway; isopentenyl diphosphate from 1-
CC deoxy-D-xylulose 5-phosphate: step 3/6.
CC -!- SIMILARITY: Belongs to the GHMP kinase family. IspE subfamily.
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DR EMBL; AM406670; CAL93373.1; -; Genomic_DNA.
DR RefSeq; YP_932260.1; NC_008702.1.
DR ProteinModelPortal; A1K3G8; -.
DR STRING; 62928.azo0756; -.
DR EnsemblBacteria; CAL93373; CAL93373; azo0756.
DR GeneID; 4609990; -.
DR KEGG; azo:azo0756; -.
DR PATRIC; 21009800; VBIAzoSp26047_0769.
DR eggNOG; COG1947; -.
DR HOGENOM; HOG000019601; -.
DR KO; K00919; -.
DR OMA; TGACVFG; -.
DR ProtClustDB; PRK00343; -.
DR UniPathway; UPA00056; UER00094.
DR GO; GO:0050515; F:4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity; IEA:HAMAP.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0019288; P:isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway; IEA:UniProtKB-UniPathway.
DR GO; GO:0016114; P:terpenoid biosynthetic process; IEA:HAMAP.
DR Gene3D; 3.30.230.10; -; 1.
DR HAMAP; MF_00061; IspE; 1; -.
DR InterPro; IPR013750; GHMP_kinase_C_dom.
DR InterPro; IPR006204; GHMP_kinase_N_dom.
DR InterPro; IPR004424; IspE.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR PANTHER; PTHR20861:SF2; PTHR20861:SF2; 1.
DR Pfam; PF08544; GHMP_kinases_C; 1.
DR Pfam; PF00288; GHMP_kinases_N; 1.
DR PIRSF; PIRSF010376; IspE; 1.
DR SUPFAM; SSF54211; Ribosomal_S5_D2-typ_fold; 1.
DR TIGRFAMs; TIGR00154; ispE; 1.
PE 3: Inferred from homology;
KW ATP-binding; Complete proteome; Isoprene biosynthesis; Kinase;
KW Nucleotide-binding; Transferase.
FT CHAIN 1 282 4-diphosphocytidyl-2-C-methyl-D-
FT erythritol kinase.
FT /FTId=PRO_0000335700.
FT NP_BIND 98 108 ATP (Potential).
FT ACT_SITE 15 15 By similarity.
FT ACT_SITE 140 140 By similarity.
SQ SEQUENCE 282 AA; 30255 MW; 449AE6E60F21A52D CRC64;
MTISGVLAGC PAPAKLNLFL HVVGRRDDGY HLLQTAFRLL DWGDRLDFRV RDDGLIRRTN
QVAGVAEDDD LVVRAARRLQ QATGTPLGAD ITLHKVLPMG GGVGGGSSDA ATTLIALNHL
WQTGLTRADL QQLGLALGAD VPFFIYGRDA FAEGVGEAFQ PLALPAAVYV VLSPEVSVPT
AEIFSAKGLT RDTPPIRIAD FAASPTRNDL QATACSRYPE VARAINWLEH YAPARMTGSG
ACVFAEVASE IEADEIVSLC PARWKAWKAK SLARHPLYGL LD
//
