ID ISPE_EUBE2 Reviewed; 288 AA.
AC C4Z205;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 1.
DT 01-MAY-2013, entry version 29.
DE RecName: Full=4-diphosphocytidyl-2-C-methyl-D-erythritol kinase;
DE Short=CMK;
DE EC=2.7.1.148;
DE AltName: Full=4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase;
GN Name=ispE; OrderedLocusNames=EUBELI_00159;
OS Eubacterium eligens (strain ATCC 27750 / VPI C15-48).
OC Bacteria; Firmicutes; Clostridia; Clostridiales; Eubacteriaceae;
OC Eubacterium.
OX NCBI_TaxID=515620;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27750 / VPI C15-48;
RX PubMed=19321416; DOI=10.1073/pnas.0901529106;
RA Mahowald M.A., Rey F.E., Seedorf H., Turnbaugh P.J., Fulton R.S.,
RA Wollam A., Shah N., Wang C., Magrini V., Wilson R.K., Cantarel B.L.,
RA Coutinho P.M., Henrissat B., Crock L.W., Russell A., Verberkmoes N.C.,
RA Hettich R.L., Gordon J.I.;
RT "Characterizing a model human gut microbiota composed of members of
RT its two dominant bacterial phyla.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:5859-5864(2009).
CC -!- FUNCTION: Catalyzes the phosphorylation of the position 2 hydroxy
CC group of 4-diphosphocytidyl-2C-methyl-D-erythritol (By
CC similarity).
CC -!- CATALYTIC ACTIVITY: ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-
CC erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-
CC D-erythritol.
CC -!- PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate
CC biosynthesis via DXP pathway; isopentenyl diphosphate from 1-
CC deoxy-D-xylulose 5-phosphate: step 3/6.
CC -!- SIMILARITY: Belongs to the GHMP kinase family. IspE subfamily.
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DR EMBL; CP001104; ACR71195.1; -; Genomic_DNA.
DR RefSeq; YP_002929642.1; NC_012778.1.
DR ProteinModelPortal; C4Z205; -.
DR STRING; 515620.EUBELI_00159; -.
DR EnsemblBacteria; ACR71195; ACR71195; EUBELI_00159.
DR GeneID; 7958324; -.
DR KEGG; eel:EUBELI_00159; -.
DR PATRIC; 21863518; VBIEubEli113401_0146.
DR eggNOG; COG1947; -.
DR HOGENOM; HOG000019600; -.
DR KO; K00919; -.
DR OMA; LMGVNEL; -.
DR ProtClustDB; PRK00128; -.
DR BioCyc; EELI515620:GH1N-975-MONOMER; -.
DR UniPathway; UPA00056; UER00094.
DR GO; GO:0050515; F:4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity; IEA:HAMAP.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0019288; P:isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway; IEA:UniProtKB-UniPathway.
DR GO; GO:0016114; P:terpenoid biosynthetic process; IEA:HAMAP.
DR Gene3D; 3.30.230.10; -; 1.
DR HAMAP; MF_00061; IspE; 1; -.
DR InterPro; IPR013750; GHMP_kinase_C_dom.
DR InterPro; IPR006204; GHMP_kinase_N_dom.
DR InterPro; IPR004424; IspE.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR PANTHER; PTHR20861:SF2; PTHR20861:SF2; 1.
DR Pfam; PF08544; GHMP_kinases_C; 1.
DR Pfam; PF00288; GHMP_kinases_N; 1.
DR PIRSF; PIRSF010376; IspE; 1.
DR SUPFAM; SSF54211; Ribosomal_S5_D2-typ_fold; 1.
DR TIGRFAMs; TIGR00154; ispE; 1.
PE 3: Inferred from homology;
KW ATP-binding; Complete proteome; Isoprene biosynthesis; Kinase;
KW Nucleotide-binding; Transferase.
FT CHAIN 1 288 4-diphosphocytidyl-2-C-methyl-D-
FT erythritol kinase.
FT /FTId=PRO_1000202379.
FT NP_BIND 95 105 ATP (Potential).
FT ACT_SITE 11 11 By similarity.
FT ACT_SITE 137 137 By similarity.
SQ SEQUENCE 288 AA; 31946 MW; 37792CED3103805D CRC64;
MESIRLKARA KINLGLDVIG RRENGYHDVR MVMQTVGLYD RIIMTRIPEE EIRIKTNIGF
LPVNENNLVY KAIMLMKNKY KLDGGIEVDL NKFIPVAAGM AGGSSDAACA LFGMNRLFEL
NVPMRELMKL GVEIGADVPY CLMRGTALAE GIGEKLTRLP DMPFCHILIA KPPVNVSTKL
VYEKLDNTDV KLHPDIDGII EAIKLKDVAL VASRMGNVLE SVTIPLYPVI DSIKKDMIEH
GAINAMMSGS GPTVFGIFPD EQSMIACQQF LRQKGEARQV YTTETFTP
//
