ID KCNH8_RAT Reviewed; 1102 AA.
AC Q9QWS8; O88877;
DT 28-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2002, sequence version 2.
DT 03-APR-2013, entry version 106.
DE RecName: Full=Potassium voltage-gated channel subfamily H member 8;
DE AltName: Full=Ether-a-go-go-like potassium channel 3;
DE Short=ELK channel 3;
DE AltName: Full=Voltage-gated potassium channel subunit Kv12.1;
GN Name=Kcnh8; Synonyms=Elk1, Elk3;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC Muroidea; Muridae; Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9714851; DOI=10.1111/j.1469-7793.1998.675bg.x;
RA Shi W., Wang H.-S., Pan Z., Wymore R.S., Cohen I.S., McKinnon D.,
RA Dixon J.E.;
RT "Cloning of a mammalian elk potassium channel gene and EAG mRNA
RT distribution in rat sympathetic ganglia.";
RL J. Physiol. (Lond.) 511:675-682(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 9-379.
RC TISSUE=Brain cortex;
RX PubMed=9824707; DOI=10.1111/j.1469-7793.1998.647ba.x;
RA Engeland B., Neu A., Ludwig J., Roeper J., Pongs O.;
RT "Cloning and functional expression of rat ether-a-go-go-like K+
RT channel genes.";
RL J. Physiol. (Lond.) 513:647-654(1998).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=10718922; DOI=10.1046/j.1365-2826.2000.00447.x;
RA Wulfsen I., Hauber H.-P., Schiemann D., Bauer C.K., Schwarz J.R.;
RT "Expression of mRNA for voltage-dependent and inward-rectifying K
RT channels in GH3/B6 cells and rat pituitary.";
RL J. Neuroendocrinol. 12:263-272(2000).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=11425889;
RA Saganich M.J., Machado E., Rudy B.;
RT "Differential expression of genes encoding subthreshold-operating
RT voltage-gated K+ channels in brain.";
RL J. Neurosci. 21:4609-4624(2001).
CC -!- FUNCTION: Pore-forming (alpha) subunit of voltage-gated potassium
CC channel. Elicits a slowly activating, outward rectifying current.
CC Channel properties may be modulated by cAMP and subunit assembly.
CC -!- SUBUNIT: The potassium channel is probably composed of a homo- or
CC heterotetrameric complex of pore-forming alpha subunits that can
CC associate with modulating beta subunits.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Detected in superior cervical, mesenteric and
CC coeliac ganglia. Expressed in brain (piriform cortex, olfactory
CC tubercle, cerebral cortex, hippocampus pyramidial cells and
CC dentate gyrus and basal ganglia of caudate/putamen and accumbens
CC nucleus). Expressed in pituitary.
CC -!- DOMAIN: The segment S4 is probably the voltage-sensor and is
CC characterized by a series of positively charged amino acids at
CC every third position.
CC -!- SIMILARITY: Belongs to the potassium channel family. H (Eag)
CC (TC 1.A.1.20) subfamily. Kv12.1/KCNH8 sub-subfamily.
CC -!- SIMILARITY: Contains 1 cyclic nucleotide-binding domain.
CC -!- SIMILARITY: Contains 1 PAC (PAS-associated C-terminal) domain.
CC -!- SIMILARITY: Contains 1 PAS (PER-ARNT-SIM) domain.
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DR EMBL; AF061957; AAC61520.1; -; mRNA.
DR EMBL; AJ007632; CAA07591.1; -; mRNA.
DR IPI; IPI00212617; -.
DR PIR; T17367; T17367.
DR RefSeq; NP_659563.1; NM_145095.1.
DR UniGene; Rn.30029; -.
DR ProteinModelPortal; Q9QWS8; -.
DR PaxDb; Q9QWS8; -.
DR GeneID; 246325; -.
DR KEGG; rno:246325; -.
DR CTD; 131096; -.
DR RGD; 2549; Kcnh8.
DR eggNOG; COG2202; -.
DR HOGENOM; HOG000230794; -.
DR HOVERGEN; HBG052232; -.
DR InParanoid; Q9QWS8; -.
DR KO; K04911; -.
DR OrthoDB; EOG47WNN0; -.
DR BindingDB; Q9QWS8; -.
DR NextBio; 623752; -.
DR ArrayExpress; Q9QWS8; -.
DR Genevestigator; Q9QWS8; -.
DR GermOnline; ENSRNOG00000012711; Rattus norvegicus.
DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR GO; GO:0003705; F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; IMP:RGD.
DR GO; GO:0005249; F:voltage-gated potassium channel activity; IMP:RGD.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IMP:RGD.
DR GO; GO:0023014; P:signal transduction by phosphorylation; IEA:GOC.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR018490; cNMP-bd-like.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR005821; Ion_trans_dom.
DR InterPro; IPR003938; K_chnl_volt-dep_EAG/ELK/ERG.
DR InterPro; IPR003950; K_chnl_volt-dep_ELK.
DR InterPro; IPR001610; PAC.
DR InterPro; IPR000014; PAS.
DR InterPro; IPR000700; PAS-assoc_C.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR Pfam; PF00027; cNMP_binding; 1.
DR Pfam; PF00520; Ion_trans; 1.
DR PRINTS; PR01463; EAGCHANLFMLY.
DR PRINTS; PR01465; ELKCHANNEL.
DR SMART; SM00100; cNMP; 1.
DR SMART; SM00086; PAC; 1.
DR SUPFAM; SSF51206; cNMP_binding; 1.
DR TIGRFAMs; TIGR00229; sensory_box; 1.
DR PROSITE; PS00888; CNMP_BINDING_1; FALSE_NEG.
DR PROSITE; PS00889; CNMP_BINDING_2; FALSE_NEG.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
DR PROSITE; PS50113; PAC; 1.
DR PROSITE; PS50112; PAS; FALSE_NEG.
PE 2: Evidence at transcript level;
KW Complete proteome; Glycoprotein; Ion channel; Ion transport; Membrane;
KW Potassium; Potassium channel; Potassium transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport; Voltage-gated channel.
FT CHAIN 1 1102 Potassium voltage-gated channel subfamily
FT H member 8.
FT /FTId=PRO_0000054020.
FT TOPO_DOM 1 225 Cytoplasmic (Potential).
FT TRANSMEM 226 246 Helical; Name=Segment S1; (Potential).
FT TOPO_DOM 247 255 Extracellular (Potential).
FT TRANSMEM 256 276 Helical; Name=Segment S2; (Potential).
FT TOPO_DOM 277 298 Cytoplasmic (Potential).
FT TRANSMEM 299 319 Helical; Name=Segment S3; (Potential).
FT TOPO_DOM 320 327 Extracellular (Potential).
FT TRANSMEM 328 348 Helical; Voltage-sensor; Name=Segment S4;
FT (Potential).
FT TOPO_DOM 349 353 Cytoplasmic (Potential).
FT TRANSMEM 354 374 Helical; Name=Segment S5; (Potential).
FT TOPO_DOM 375 419 Extracellular (Potential).
FT INTRAMEM 420 440 Pore-forming; Name=Segment H5;
FT (Potential).
FT TOPO_DOM 441 448 Extracellular (Potential).
FT TRANSMEM 449 469 Helical; Name=Segment S6; (Potential).
FT TOPO_DOM 470 1102 Cytoplasmic (Potential).
FT DOMAIN 18 90 PAS.
FT DOMAIN 93 145 PAC.
FT NP_BIND 551 668 cNMP.
FT MOTIF 434 439 Selectivity filter (By similarity).
FT COMPBIAS 711 722 Poly-Glu.
FT CARBOHYD 320 320 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 409 409 N-linked (GlcNAc...) (Potential).
FT CONFLICT 71 71 F -> L (in Ref. 2; CAA07591).
FT CONFLICT 187 187 K -> N (in Ref. 2; CAA07591).
FT CONFLICT 296 296 I -> T (in Ref. 2; CAA07591).
FT CONFLICT 370 370 M -> I (in Ref. 2; CAA07591).
SQ SEQUENCE 1102 AA; 123231 MW; A135CC36E2E7F1A3 CRC64;
MPVMKGLLAP QNTFLDTIAT RFDGTHSNFI LANAQVAKGF PIVYCSDGFC ELAGFARTEV
MQKSCSCKFL FGVETNEQLM LQIEKSLEEK VEFKGEIMFY KKNGAPFWCL LDIVPIKNEK
GDVVLFLASF KDITDTKVKI TSEDKKEDRA KGRSRAGSHF DSARRRSRAV LYHISGHLQR
REKNKLKINN NVFVDKPAFP EYKVSDAKKS KFILLHFSTF KAGWDWLILL ATFYVAVTVP
YNVCFIGNED LSTTRSTTVS DIAVEILFII DIILNFRTTY VSKSGQVIFE ARSICIHYVT
TWFIIDLIAA LPFDLLYAFN VTVVSLVHLL KTVRLLRLLR LLQKLDRYSQ HSTIVLTLLM
SMFALLAHWM ACIWYVIGKM EREDNSLLKW EVGWLHELGK RLESPYYGNN TLGGPSIRSA
YIAALYFTLS SLTSVGFGNV SANTDAEKIF SICTMLIGAL MHALVFGNVT AIIQRMYSRW
SLYHTRTKDL KDFIRVHHLP QQLKQRMLEY FQTTWSVNNG IDSNELLKDF PDELRSDITM
HLNKEILQLS LFECASRGCL RSLSLHIKTS FCAPGEYLLR QGDALQAIYF VCSGSMEVLK
DSMVLAILGK GDLIGANLSI KDQVIKTNAD VKALTYCDLQ CIILKGLFEV LGLYPEYAHK
FVEDIQHDLT YNLREGHESD VISRLSNKST VPQAEPKGNG SIKKRLPSIV EDEEEEEVEE
EETTSLSPIY TRGSSVSHSK KTGSSKSYLG LSLKQLTSGT VPFHSPIRVS SANSPKTKQE
ADPPNHGTRK EKNLKVQLCS LGTAGTPELS PRIVDGIEDG NSSEETQTFD FGSEQIRPEP
RISPSLGESE IGAAFLFIKA EETKQQINKL NSEVTTLTQE VSQLGKDMRS IMQLLENILS
PQQPSQFCSL HPTSICPSRE SFQTRVSWSA HQPCLHLQAN GAHLYHGNVT SDIWSVDPSL
VGSNPQRTEA HEQSPVDSEL HHSPNLAYSP SHCQVIQEGH LQFLRCISPH SDTTLTPLQS
ISATLSSSVC SSSETSLHLV LPSRSEEGSI THGPVSSFSL ENLPGSWDRE GMMSASTEPL
ENFPVEVVTS TADVKDSKAI NV
//
