ID LIGC_MYCTU Reviewed; 358 AA.
AC L0TDE1;
DT 19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2013, sequence version 1.
DT 27-MAR-2024, entry version 59.
DE RecName: Full=DNA ligase C;
DE EC=6.5.1.1;
DE AltName: Full=Polydeoxyribonucleotide synthase [ATP];
GN Name=ligC; OrderedLocusNames=Rv3731;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX PubMed=14985346; DOI=10.1074/jbc.m401841200;
RA Gong C., Martins A., Bongiorno P., Glickman M., Shuman S.;
RT "Biochemical and genetic analysis of the four DNA ligases of
RT mycobacteria.";
RL J. Biol. Chem. 279:20594-20606(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011445;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- FUNCTION: DNA ligase that seals nicks in double-stranded DNA during DNA
CC replication, DNA recombination and DNA repair. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP +
CC diphosphate.; EC=6.5.1.1;
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000250};
CC -!- DISRUPTION PHENOTYPE: Not essential for growth (in strain Erdman).
CC {ECO:0000269|PubMed:14985346}.
CC -!- SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
CC {ECO:0000305}.
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DR EMBL; AL123456; CCP46558.1; -; Genomic_DNA.
DR RefSeq; NP_218248.1; NC_000962.3.
DR RefSeq; WP_003917239.1; NZ_NVQJ01000009.1.
DR AlphaFoldDB; L0TDE1; -.
DR SMR; L0TDE1; -.
DR STRING; 83332.Rv3731; -.
DR PaxDb; 83332-Rv3731; -.
DR DNASU; 885771; -.
DR GeneID; 885771; -.
DR KEGG; mtu:Rv3731; -.
DR TubercuList; Rv3731; -.
DR eggNOG; COG1793; Bacteria.
DR InParanoid; L0TDE1; -.
DR OrthoDB; 9770771at2; -.
DR PhylomeDB; L0TDE1; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003910; F:DNA ligase (ATP) activity; IEA:UniProtKB-EC.
DR GO; GO:0006310; P:DNA recombination; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR CDD; cd07905; Adenylation_DNA_ligase_LigC; 1.
DR CDD; cd07970; OBF_DNA_ligase_LigC; 1.
DR Gene3D; 3.30.470.30; DNA ligase/mRNA capping enzyme; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR InterPro; IPR044119; Adenylation_LigC-like.
DR InterPro; IPR012309; DNA_ligase_ATP-dep_C.
DR InterPro; IPR012310; DNA_ligase_ATP-dep_cent.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR044117; OBF_LigC-like.
DR PANTHER; PTHR45674; DNA LIGASE 1/3 FAMILY MEMBER; 1.
DR PANTHER; PTHR45674:SF9; DNA LIGASE 3; 1.
DR Pfam; PF04679; DNA_ligase_A_C; 1.
DR Pfam; PF01068; DNA_ligase_A_M; 1.
DR SUPFAM; SSF56091; DNA ligase/mRNA capping enzyme, catalytic domain; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR PROSITE; PS50160; DNA_LIGASE_A3; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Ligase; Nucleotide-binding; Reference proteome.
FT CHAIN 1..358
FT /note="DNA ligase C"
FT /id="PRO_0000425953"
FT ACT_SITE 29
FT /note="N6-AMP-lysine intermediate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 358 AA; 40160 MW; 8EBA33D1A57CAA4E CRC64;
MQLPVMPPVS PMLAKSVTAI PPDASYEPKW DGFRSICFRD GDQVELGSRN ERPMTRYFPE
LVAAIRAELP HRCVIDGEII IATDHGLDFE ALQQRIHPAE SRVRMLADRT PASFIAFDLL
ALGDDDYTGR PFSERRAALV DAVTGSGADA DLSIHVTPAT TDMATAQRWF SEFEGAGLDG
VIAKPPHITY QPDKRVMFKI KHLRTADCVV AGYRVHKSGS DAIGSLLLGL YQEDGQLASV
GVIGAFPMAE RRRLLTELQP LVTSFDDHPW NWAAHVAGQR TPRKNEFSRW NVGKDLSFVP
LRPERVVEVR YDRMEGARFR HTAQFNRWRP DRDPRSCSYA QLERPLTVSL SDIVPGLR
//
