ID   MDHC_MAIZE              Reviewed;         332 AA.
AC   Q08062;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 2.
DT   29-MAY-2013, entry version 104.
DE   RecName: Full=Malate dehydrogenase, cytoplasmic;
DE            EC=1.1.1.37;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae;
OC   PACMAD clade; Panicoideae; Andropogoneae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Yedan 4; TISSUE=Leaf;
RA   Hu J., Zhao X., Yuan Z., Qian X., Yang J.;
RT   "Cloning and characterization of cytoplasmic malate dehydrogenase
RT   over-expressed in hybrid maize.";
RL   Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 4-73.
RC   TISSUE=Leaf, and Sheath;
RX   PubMed=8278499; DOI=10.1104/pp.101.1.329;
RA   Keith C.S., Hoang D.O., Barrett B.M., Feigelman B., Nelson M.C.,
RA   Thai H., Baysdorfer C.;
RT   "Partial sequence analysis of 130 randomly selected maize cDNA
RT   clones.";
RL   Plant Physiol. 101:329-332(1993).
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = oxaloacetate + NADH.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 2 family.
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DR   EMBL; AF007581; AAB64290.1; -; mRNA.
DR   EMBL; M95069; AAA18556.2; -; mRNA.
DR   PIR; T02935; T02935.
DR   PIR; T03650; T03650.
DR   RefSeq; NP_001105603.1; NM_001112133.1.
DR   UniGene; Zm.103385; -.
DR   UniGene; Zm.82624; -.
DR   ProteinModelPortal; Q08062; -.
DR   SMR; Q08062; 4-331.
DR   PRIDE; Q08062; -.
DR   ProMEX; Q08062; -.
DR   GeneID; 542598; -.
DR   KEGG; zma:542598; -.
DR   Gramene; Q08062; -.
DR   HOGENOM; HOG000220953; -.
DR   KO; K00025; -.
DR   OMA; NCLIASK; -.
DR   GO; GO:0048046; C:apoplast; IEA:EnsemblPlants/Gramene.
DR   GO; GO:0009570; C:chloroplast stroma; IEA:EnsemblPlants/Gramene.
DR   GO; GO:0005634; C:nucleus; IEA:EnsemblPlants/Gramene.
DR   GO; GO:0005886; C:plasma membrane; IEA:EnsemblPlants/Gramene.
DR   GO; GO:0005773; C:vacuole; IEA:EnsemblPlants/Gramene.
DR   GO; GO:0030060; F:L-malate dehydrogenase activity; IEA:EC.
DR   GO; GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0046686; P:response to cadmium ion; IEA:EnsemblPlants/Gramene.
DR   GO; GO:0009651; P:response to salt stress; IEA:EnsemblPlants/Gramene.
DR   GO; GO:0010043; P:response to zinc ion; IEA:EnsemblPlants/Gramene.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.720; -; 1.
DR   Gene3D; 3.90.110.10; -; 1.
DR   InterPro; IPR001557; L-lactate/malate_DH.
DR   InterPro; IPR022383; Lactate/malate_DH_C.
DR   InterPro; IPR001236; Lactate/malate_DH_N.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   InterPro; IPR001252; Malate_DH_AS.
DR   InterPro; IPR011274; Malate_DH_NAD-dep_euk.
DR   InterPro; IPR010945; Malate_DH_type2.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   PANTHER; PTHR23382; PTHR23382; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   SUPFAM; SSF56327; Lactate_DH/Glyco_hydro_4_C; 1.
DR   TIGRFAMs; TIGR01759; MalateDH-SF1; 1.
DR   TIGRFAMs; TIGR01758; MDH_euk_cyt; 1.
DR   PROSITE; PS00068; MDH; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; NAD; Oxidoreductase; Tricarboxylic acid cycle.
FT   CHAIN         1    332       Malate dehydrogenase, cytoplasmic.
FT                                /FTId=PRO_0000113415.
FT   NP_BIND      12     18       NAD (By similarity).
FT   NP_BIND     130    132       NAD (By similarity).
FT   ACT_SITE    188    188       Proton acceptor (By similarity).
FT   BINDING      93     93       Substrate (By similarity).
FT   BINDING      99     99       Substrate (By similarity).
FT   BINDING     106    106       NAD (By similarity).
FT   BINDING     113    113       NAD (By similarity).
FT   BINDING     132    132       Substrate (By similarity).
FT   BINDING     163    163       Substrate (By similarity).
SQ   SEQUENCE   332 AA;  35590 MW;  05D82B33FF0CE26F CRC64;
     MAKEPMRVLV TGAAGQIGYA LVPMIARGVM LGADQPVILH MLDIPPAAEA LNGVKMELVD
     AAFPLLKGVV ATTDVVEACT GVNVAVMVGG FPRKEGMERK DVMSKNVSIY KSQASALEAH
     AAPNCKVLVV ANPANTNALI LKEFAPSIPE KNVTCLTRLD HNRALGQISE RLNVQVSDVK
     NVIIWGNHSS SQYPDVNHAT VKTSTGEKPV RELVSDDEWL NGEFITTVQQ RGAAIIKARK
     FSSALSAASS ACDHIRDWVL GTPEGTFVSM GVYSDGSYGV PSGLIYSFPV TCSGGEWKIV
     QGLPIDEFSR KKMDATAQEL TEEKTLAYSC LE
//

ID   C4IZW9_MAIZE            Unreviewed;       332 AA.
AC   C4IZW9;
DT   07-JUL-2009, integrated into UniProtKB/TrEMBL.
DT   07-JUL-2009, sequence version 1.
DT   29-MAY-2013, entry version 31.
DE   RecName: Full=Malate dehydrogenase;
DE            EC=1.1.1.37;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae;
OC   PACMAD clade; Panicoideae; Andropogoneae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=B73;
RX   PubMed=19936069; DOI=10.1371/journal.pgen.1000740;
RA   Soderlund C., Descour A., Kudrna D., Bomhoff M., Boyd L., Currie J.,
RA   Angelova A., Collura K., Wissotski M., Ashley E., Morrow D.,
RA   Fernandes J., Walbot V., Yu Y.;
RT   "Sequencing, mapping, and analysis of 27,455 maize full-length
RT   cDNAs.";
RL   PLoS Genet. 5:E1000740-E1000740(2009).
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = oxaloacetate + NADH.
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 2 family.
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DR   EMBL; BT084116; ACR34469.1; -; mRNA.
DR   RefSeq; NP_001105603.1; NM_001112133.1.
DR   UniGene; Zm.82624; -.
DR   ProteinModelPortal; C4IZW9; -.
DR   SMR; C4IZW9; 4-331.
DR   PRIDE; C4IZW9; -.
DR   GeneID; 542598; -.
DR   KEGG; zma:542598; -.
DR   Gramene; C4IZW9; -.
DR   KO; K00025; -.
DR   OMA; NCLIASK; -.
DR   GO; GO:0048046; C:apoplast; IEA:EnsemblPlants/Gramene.
DR   GO; GO:0009570; C:chloroplast stroma; IEA:EnsemblPlants/Gramene.
DR   GO; GO:0005634; C:nucleus; IEA:EnsemblPlants/Gramene.
DR   GO; GO:0005886; C:plasma membrane; IEA:EnsemblPlants/Gramene.
DR   GO; GO:0005773; C:vacuole; IEA:EnsemblPlants/Gramene.
DR   GO; GO:0030060; F:L-malate dehydrogenase activity; IEA:EC.
DR   GO; GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0046686; P:response to cadmium ion; IEA:EnsemblPlants/Gramene.
DR   GO; GO:0009651; P:response to salt stress; IEA:EnsemblPlants/Gramene.
DR   GO; GO:0010043; P:response to zinc ion; IEA:EnsemblPlants/Gramene.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.720; -; 1.
DR   Gene3D; 3.90.110.10; -; 1.
DR   InterPro; IPR001557; L-lactate/malate_DH.
DR   InterPro; IPR022383; Lactate/malate_DH_C.
DR   InterPro; IPR001236; Lactate/malate_DH_N.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   InterPro; IPR001252; Malate_DH_AS.
DR   InterPro; IPR011274; Malate_DH_NAD-dep_euk.
DR   InterPro; IPR010945; Malate_DH_type2.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   PANTHER; PTHR23382; PTHR23382; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   SUPFAM; SSF56327; Lactate_DH/Glyco_hydro_4_C; 1.
DR   TIGRFAMs; TIGR01759; MalateDH-SF1; 1.
DR   TIGRFAMs; TIGR01758; MDH_euk_cyt; 1.
DR   PROSITE; PS00068; MDH; 1.
PE   2: Evidence at transcript level;
KW   NAD; Oxidoreductase; Tricarboxylic acid cycle.
SQ   SEQUENCE   332 AA;  35590 MW;  05D82B33FF0CE26F CRC64;
     MAKEPMRVLV TGAAGQIGYA LVPMIARGVM LGADQPVILH MLDIPPAAEA LNGVKMELVD
     AAFPLLKGVV ATTDVVEACT GVNVAVMVGG FPRKEGMERK DVMSKNVSIY KSQASALEAH
     AAPNCKVLVV ANPANTNALI LKEFAPSIPE KNVTCLTRLD HNRALGQISE RLNVQVSDVK
     NVIIWGNHSS SQYPDVNHAT VKTSTGEKPV RELVSDDEWL NGEFITTVQQ RGAAIIKARK
     FSSALSAASS ACDHIRDWVL GTPEGTFVSM GVYSDGSYGV PSGLIYSFPV TCSGGEWKIV
     QGLPIDEFSR KKMDATAQEL TEEKTLAYSC LE
//