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Database: UniProt/SWISS-PROT
Entry: MDH_THEFY
LinkDB: MDH_THEFY
Original site: MDH_THEFY 
ID   MDH_THEFY               Reviewed;         330 AA.
AC   Q47TT4;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   25-OCT-2017, entry version 86.
DE   RecName: Full=Malate dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01517};
DE            EC=1.1.1.37 {ECO:0000255|HAMAP-Rule:MF_01517};
GN   Name=mdh {ECO:0000255|HAMAP-Rule:MF_01517};
GN   OrderedLocusNames=Tfu_0092;
OS   Thermobifida fusca (strain YX).
OC   Bacteria; Actinobacteria; Streptosporangiales; Nocardiopsaceae;
OC   Thermobifida.
OX   NCBI_TaxID=269800;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YX;
RX   PubMed=17209016; DOI=10.1128/JB.01899-06;
RA   Lykidis A., Mavromatis K., Ivanova N., Anderson I., Land M.,
RA   DiBartolo G., Martinez M., Lapidus A., Lucas S., Copeland A.,
RA   Richardson P., Wilson D.B., Kyrpides N.;
RT   "Genome sequence and analysis of the soil cellulolytic actinomycete
RT   Thermobifida fusca YX.";
RL   J. Bacteriol. 189:2477-2486(2007).
CC   -!- FUNCTION: Catalyzes the reversible oxidation of malate to
CC       oxaloacetate. {ECO:0000255|HAMAP-Rule:MF_01517}.
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = oxaloacetate + NADH.
CC       {ECO:0000255|HAMAP-Rule:MF_01517}.
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 2 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01517}.
DR   EMBL; CP000088; AAZ54130.1; -; Genomic_DNA.
DR   RefSeq; WP_011290539.1; NC_007333.1.
DR   ProteinModelPortal; Q47TT4; -.
DR   SMR; Q47TT4; -.
DR   STRING; 269800.Tfu_0092; -.
DR   PRIDE; Q47TT4; -.
DR   EnsemblBacteria; AAZ54130; AAZ54130; Tfu_0092.
DR   KEGG; tfu:Tfu_0092; -.
DR   eggNOG; ENOG4105D9Z; Bacteria.
DR   eggNOG; COG0039; LUCA.
DR   HOGENOM; HOG000220953; -.
DR   KO; K00024; -.
DR   OMA; RPRTKGM; -.
DR   OrthoDB; POG091H03R4; -.
DR   Proteomes; UP000000434; Chromosome.
DR   GO; GO:0030060; F:L-malate dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.110.10; -; 1.
DR   HAMAP; MF_01517; Malate_dehydrog_2; 1.
DR   InterPro; IPR001557; L-lactate/malate_DH.
DR   InterPro; IPR022383; Lactate/malate_DH_C.
DR   InterPro; IPR001236; Lactate/malate_DH_N.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   InterPro; IPR001252; Malate_DH_AS.
DR   InterPro; IPR010945; Malate_DH_type2.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   PANTHER; PTHR23382; PTHR23382; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   SUPFAM; SSF56327; SSF56327; 1.
DR   TIGRFAMs; TIGR01759; MalateDH-SF1; 1.
DR   PROSITE; PS00068; MDH; 1.
PE   3: Inferred from homology;
KW   Complete proteome; NAD; Oxidoreductase; Reference proteome;
KW   Tricarboxylic acid cycle.
FT   CHAIN         1    330       Malate dehydrogenase.
FT                                /FTId=PRO_0000113396.
FT   NP_BIND      12     18       NAD. {ECO:0000255|HAMAP-Rule:MF_01517}.
FT   NP_BIND     130    132       NAD. {ECO:0000255|HAMAP-Rule:MF_01517}.
FT   ACT_SITE    188    188       Proton acceptor. {ECO:0000255|HAMAP-
FT                                Rule:MF_01517}.
FT   BINDING      93     93       Substrate. {ECO:0000255|HAMAP-
FT                                Rule:MF_01517}.
FT   BINDING      99     99       Substrate. {ECO:0000255|HAMAP-
FT                                Rule:MF_01517}.
FT   BINDING     106    106       NAD. {ECO:0000255|HAMAP-Rule:MF_01517}.
FT   BINDING     113    113       NAD. {ECO:0000255|HAMAP-Rule:MF_01517}.
FT   BINDING     132    132       Substrate. {ECO:0000255|HAMAP-
FT                                Rule:MF_01517}.
FT   BINDING     163    163       Substrate. {ECO:0000255|HAMAP-
FT                                Rule:MF_01517}.
SQ   SEQUENCE   330 AA;  35126 MW;  9DC57DB6E5917012 CRC64;
     MAKAPVNVTV TGAAGQIGYA LLFRIASGQL LGVDTPVRLR LLEIPQAIKA AEGTAMELDD
     CAFPLLAGVD IYDDPRKAFD GVNVALLVGA RPRTKGMERR DLLEANGGIF GPQGAAINDG
     AADDVRVLVV GNPANTNALI AQAHAPDIPA DRFTAMTRLD HNRALSQLAA KLNVSVSDIK
     KLTIWGNHSA TQYPDIFHAE VNGRSAVEAV NDEEWLRDTF IPTVAKRGAA IIEARGASSA
     ASAANAAIDH VYDWVNGTPE GDWTSVALPS DGSYGVPEGL VSSFPVVSRN GSWEIVQGLE
     INEFSRERID ASVRELEEER EAVRALGLIK
//
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