GenomeNet

Database: UniProt/SWISS-PROT
Entry: NANB_STRPN
LinkDB: NANB_STRPN
Original site: NANB_STRPN 
ID   NANB_STRPN              Reviewed;         697 AA.
AC   Q54727;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   26-SEP-2001, sequence version 2.
DT   05-JUL-2017, entry version 132.
DE   RecName: Full=Sialidase B;
DE            EC=3.2.1.18;
DE   AltName: Full=Neuraminidase B;
DE   Flags: Precursor;
GN   Name=nanB; OrderedLocusNames=SP_1687;
OS   Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=170187;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Serotype 6;
RX   PubMed=8759848; DOI=10.1128/jb.178.16.4854-4860.1996;
RA   Berry A.M., Lock R.A., Paton J.C.;
RT   "Cloning and characterization of nanB, a second Streptococcus
RT   pneumoniae neuraminidase gene, and purification of the NanB enzyme
RT   from recombinant Escherichia coli.";
RL   J. Bacteriol. 178:4854-4860(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-334 / TIGR4;
RX   PubMed=11463916; DOI=10.1126/science.1061217;
RA   Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D.,
RA   Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J.,
RA   Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D.,
RA   Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D.,
RA   Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L.,
RA   McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T.,
RA   Hickey E.K., Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C.,
RA   Dougherty B.A., Morrison D.A., Hollingshead S.K., Fraser C.M.;
RT   "Complete genome sequence of a virulent isolate of Streptococcus
RT   pneumoniae.";
RL   Science 293:498-506(2001).
CC   -!- CATALYTIC ACTIVITY: Hydrolysis of alpha-(2->3)-, alpha-(2->6)-,
CC       alpha-(2->8)- glycosidic linkages of terminal sialic acid residues
CC       in oligosaccharides, glycoproteins, glycolipids, colominic acid
CC       and synthetic substrates.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 33 family.
CC       {ECO:0000305}.
DR   EMBL; U43526; AAC44396.1; -; Genomic_DNA.
DR   EMBL; AE005672; AAK75766.1; -; Genomic_DNA.
DR   PIR; E95196; E95196.
DR   RefSeq; WP_001042179.1; NZ_AKVY01000001.1.
DR   PDB; 2JKB; X-ray; 1.54 A; A=30-697.
DR   PDB; 2VW0; X-ray; 2.30 A; A=1-697.
DR   PDB; 2VW1; X-ray; 2.39 A; A=1-697.
DR   PDB; 2VW2; X-ray; 1.70 A; A=1-697.
DR   PDB; 4FOQ; X-ray; 1.99 A; A=1-697.
DR   PDB; 4FOV; X-ray; 2.29 A; A=1-697.
DR   PDB; 4FOW; X-ray; 2.10 A; A=1-697.
DR   PDB; 4FOY; X-ray; 1.84 A; A=1-697.
DR   PDB; 4FP2; X-ray; 2.05 A; A=1-697.
DR   PDB; 4FP3; X-ray; 2.74 A; A=1-697.
DR   PDB; 4FPC; X-ray; 2.32 A; A=1-697.
DR   PDB; 4FPE; X-ray; 2.18 A; A=1-697.
DR   PDB; 4FPF; X-ray; 2.23 A; A=1-697.
DR   PDB; 4FPG; X-ray; 2.58 A; A=1-697.
DR   PDB; 4FPH; X-ray; 1.94 A; A=1-697.
DR   PDB; 4FPJ; X-ray; 1.98 A; A=1-697.
DR   PDB; 4FPK; X-ray; 2.40 A; A=1-697.
DR   PDB; 4FPL; X-ray; 2.10 A; A=1-697.
DR   PDB; 4FPO; X-ray; 2.59 A; B=1-697.
DR   PDB; 4FPY; X-ray; 2.18 A; A=1-697.
DR   PDB; 4FQ4; X-ray; 1.84 A; A=1-697.
DR   PDB; 4XE9; X-ray; 1.84 A; A=39-696.
DR   PDB; 4XHB; X-ray; 1.88 A; A=39-696.
DR   PDB; 4XHX; X-ray; 2.10 A; A=39-696.
DR   PDB; 4XIK; X-ray; 1.91 A; A=39-696.
DR   PDB; 4XIL; X-ray; 1.90 A; A=39-696.
DR   PDB; 4XIO; X-ray; 1.80 A; A=39-696.
DR   PDB; 4XJ8; X-ray; 2.75 A; A=39-696.
DR   PDB; 4XJ9; X-ray; 1.98 A; A=39-696.
DR   PDB; 4XJA; X-ray; 1.98 A; A=39-696.
DR   PDB; 4XJU; X-ray; 1.94 A; A=39-696.
DR   PDB; 4XJW; X-ray; 1.53 A; A=39-696.
DR   PDB; 4XJZ; X-ray; 1.56 A; A=39-696.
DR   PDB; 4XMA; X-ray; 2.09 A; A=39-696.
DR   PDB; 4XMI; X-ray; 1.97 A; A=39-696.
DR   PDB; 4XOG; X-ray; 2.09 A; A=37-694.
DR   PDB; 4XYX; X-ray; 2.10 A; A=40-697.
DR   PDBsum; 2JKB; -.
DR   PDBsum; 2VW0; -.
DR   PDBsum; 2VW1; -.
DR   PDBsum; 2VW2; -.
DR   PDBsum; 4FOQ; -.
DR   PDBsum; 4FOV; -.
DR   PDBsum; 4FOW; -.
DR   PDBsum; 4FOY; -.
DR   PDBsum; 4FP2; -.
DR   PDBsum; 4FP3; -.
DR   PDBsum; 4FPC; -.
DR   PDBsum; 4FPE; -.
DR   PDBsum; 4FPF; -.
DR   PDBsum; 4FPG; -.
DR   PDBsum; 4FPH; -.
DR   PDBsum; 4FPJ; -.
DR   PDBsum; 4FPK; -.
DR   PDBsum; 4FPL; -.
DR   PDBsum; 4FPO; -.
DR   PDBsum; 4FPY; -.
DR   PDBsum; 4FQ4; -.
DR   PDBsum; 4XE9; -.
DR   PDBsum; 4XHB; -.
DR   PDBsum; 4XHX; -.
DR   PDBsum; 4XIK; -.
DR   PDBsum; 4XIL; -.
DR   PDBsum; 4XIO; -.
DR   PDBsum; 4XJ8; -.
DR   PDBsum; 4XJ9; -.
DR   PDBsum; 4XJA; -.
DR   PDBsum; 4XJU; -.
DR   PDBsum; 4XJW; -.
DR   PDBsum; 4XJZ; -.
DR   PDBsum; 4XMA; -.
DR   PDBsum; 4XMI; -.
DR   PDBsum; 4XOG; -.
DR   PDBsum; 4XYX; -.
DR   ProteinModelPortal; Q54727; -.
DR   SMR; Q54727; -.
DR   CAZy; CBM40; Carbohydrate-Binding Module Family 40.
DR   CAZy; GH33; Glycoside Hydrolase Family 33.
DR   EnsemblBacteria; AAK75766; AAK75766; SP_1687.
DR   KEGG; spn:SP_1687; -.
DR   eggNOG; COG4409; LUCA.
DR   HOGENOM; HOG000285031; -.
DR   KO; K01186; -.
DR   OMA; IFLMADM; -.
DR   BRENDA; 3.2.1.18; 1960.
DR   SABIO-RK; Q54727; -.
DR   EvolutionaryTrace; Q54727; -.
DR   PRO; PR:Q54727; -.
DR   Proteomes; UP000000585; Chromosome.
DR   GO; GO:0052794; F:exo-alpha-(2->3)-sialidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0052795; F:exo-alpha-(2->6)-sialidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0052796; F:exo-alpha-(2->8)-sialidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   InterPro; IPR002860; BNR_rpt.
DR   InterPro; IPR013320; ConA-like_dom.
DR   InterPro; IPR004124; Glyco_hydro_33_N.
DR   InterPro; IPR026856; Sialidase_fam.
DR   InterPro; IPR011040; Sialidases.
DR   PANTHER; PTHR10628; PTHR10628; 1.
DR   Pfam; PF02012; BNR; 1.
DR   Pfam; PF13088; BNR_2; 1.
DR   Pfam; PF02973; Sialidase; 1.
DR   SUPFAM; SSF49899; SSF49899; 1.
DR   SUPFAM; SSF50939; SSF50939; 2.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; Glycosidase; Hydrolase; Repeat;
KW   Signal.
FT   SIGNAL        1     29
FT   CHAIN        30    697       Sialidase B.
FT                                /FTId=PRO_0000012038.
FT   REPEAT      280    291       BNR 1.
FT   REPEAT      462    473       BNR 2.
FT   REPEAT      517    528       BNR 3.
FT   REPEAT      566    577       BNR 4.
FT   ACT_SITE    270    270       Proton acceptor. {ECO:0000250}.
FT   ACT_SITE    541    541       {ECO:0000255}.
FT   ACT_SITE    653    653       Nucleophile. {ECO:0000250}.
FT   BINDING     245    245       Substrate. {ECO:0000250}.
FT   BINDING     557    557       Substrate. {ECO:0000250}.
FT   BINDING     619    619       Substrate. {ECO:0000250}.
FT   CONFLICT    121    121       K -> E (in Ref. 1; AAC44396).
FT                                {ECO:0000305}.
FT   CONFLICT    206    206       D -> G (in Ref. 1; AAC44396).
FT                                {ECO:0000305}.
FT   CONFLICT    222    222       T -> N (in Ref. 1; AAC44396).
FT                                {ECO:0000305}.
FT   CONFLICT    393    393       N -> D (in Ref. 1; AAC44396).
FT                                {ECO:0000305}.
FT   CONFLICT    639    639       K -> R (in Ref. 1; AAC44396).
FT                                {ECO:0000305}.
FT   STRAND       42     53       {ECO:0000244|PDB:4XJW}.
FT   HELIX        58     61       {ECO:0000244|PDB:4XJW}.
FT   TURN         62     64       {ECO:0000244|PDB:4XJW}.
FT   STRAND       70     77       {ECO:0000244|PDB:4XJW}.
FT   STRAND       83     91       {ECO:0000244|PDB:4XJW}.
FT   STRAND      101    107       {ECO:0000244|PDB:4XJW}.
FT   STRAND      112    118       {ECO:0000244|PDB:4XJW}.
FT   TURN        119    122       {ECO:0000244|PDB:4XJW}.
FT   STRAND      123    131       {ECO:0000244|PDB:4XJW}.
FT   STRAND      136    141       {ECO:0000244|PDB:2VW1}.
FT   STRAND      144    151       {ECO:0000244|PDB:4XJW}.
FT   TURN        152    155       {ECO:0000244|PDB:4XJW}.
FT   STRAND      156    161       {ECO:0000244|PDB:4XJW}.
FT   STRAND      164    170       {ECO:0000244|PDB:4XJW}.
FT   HELIX       177    179       {ECO:0000244|PDB:4XJW}.
FT   STRAND      185    193       {ECO:0000244|PDB:4XJW}.
FT   STRAND      196    199       {ECO:0000244|PDB:4XJW}.
FT   STRAND      202    213       {ECO:0000244|PDB:4XJW}.
FT   HELIX       217    220       {ECO:0000244|PDB:4XJW}.
FT   STRAND      242    245       {ECO:0000244|PDB:4XJW}.
FT   STRAND      257    266       {ECO:0000244|PDB:4XJW}.
FT   STRAND      271    273       {ECO:0000244|PDB:4XJW}.
FT   STRAND      275    284       {ECO:0000244|PDB:4XJW}.
FT   STRAND      293    296       {ECO:0000244|PDB:4XJW}.
FT   TURN        312    316       {ECO:0000244|PDB:4XJW}.
FT   STRAND      324    332       {ECO:0000244|PDB:4XJW}.
FT   TURN        334    336       {ECO:0000244|PDB:4XJW}.
FT   STRAND      339    346       {ECO:0000244|PDB:4XJW}.
FT   TURN        352    354       {ECO:0000244|PDB:4XJW}.
FT   STRAND      361    365       {ECO:0000244|PDB:4XJW}.
FT   STRAND      368    375       {ECO:0000244|PDB:4XJW}.
FT   STRAND      383    385       {ECO:0000244|PDB:4XJW}.
FT   HELIX       387    389       {ECO:0000244|PDB:4XJW}.
FT   STRAND      390    393       {ECO:0000244|PDB:4XJW}.
FT   TURN        394    397       {ECO:0000244|PDB:4XJW}.
FT   STRAND      398    404       {ECO:0000244|PDB:4XJW}.
FT   STRAND      408    412       {ECO:0000244|PDB:4XJW}.
FT   STRAND      418    425       {ECO:0000244|PDB:4XJW}.
FT   STRAND      432    441       {ECO:0000244|PDB:4XJW}.
FT   STRAND      446    448       {ECO:0000244|PDB:4FPG}.
FT   STRAND      450    452       {ECO:0000244|PDB:4XJW}.
FT   STRAND      458    466       {ECO:0000244|PDB:4XJW}.
FT   STRAND      467    469       {ECO:0000244|PDB:4FP3}.
FT   STRAND      483    485       {ECO:0000244|PDB:4FPO}.
FT   STRAND      492    494       {ECO:0000244|PDB:4XJW}.
FT   STRAND      499    502       {ECO:0000244|PDB:4XIL}.
FT   STRAND      504    510       {ECO:0000244|PDB:4XJW}.
FT   STRAND      513    521       {ECO:0000244|PDB:4XJW}.
FT   STRAND      527    532       {ECO:0000244|PDB:4XJW}.
FT   STRAND      542    548       {ECO:0000244|PDB:4XJW}.
FT   STRAND      551    556       {ECO:0000244|PDB:4XJW}.
FT   STRAND      559    562       {ECO:0000244|PDB:4XJW}.
FT   STRAND      564    570       {ECO:0000244|PDB:4XJW}.
FT   STRAND      573    575       {ECO:0000244|PDB:4FP3}.
FT   STRAND      594    597       {ECO:0000244|PDB:4XJW}.
FT   STRAND      607    613       {ECO:0000244|PDB:4XJW}.
FT   STRAND      616    620       {ECO:0000244|PDB:4XJW}.
FT   STRAND      622    629       {ECO:0000244|PDB:4XJW}.
FT   TURN        631    633       {ECO:0000244|PDB:4XJW}.
FT   STRAND      636    645       {ECO:0000244|PDB:4XJW}.
FT   STRAND      653    658       {ECO:0000244|PDB:4XJW}.
FT   STRAND      664    669       {ECO:0000244|PDB:4XJW}.
FT   STRAND      676    678       {ECO:0000244|PDB:2VW1}.
FT   STRAND      685    690       {ECO:0000244|PDB:4XJW}.
FT   HELIX       692    695       {ECO:0000244|PDB:4XJW}.
SQ   SEQUENCE   697 AA;  77695 MW;  A0D9F9AFDE46AFD7 CRC64;
     MNKRGLYSKL GISVVGISLL MGVPTLIHAN ELNYGQLSIS PIFQGGSYQL NNKSIDISSL
     LLDKLSGESQ TVVMKFKADK PNSLQALFGL SNSKAGFKNN YFSIFMRDSG EIGVEIRDAQ
     KGINYLFSRP ASLWGKHKGQ AVENTLVFVS DSKDKTYTMY VNGIEVFSET VDTFLPISNI
     NGIDKATLGA VNREGKEHYL AKGSIDEISL FNKAISDQEV STIPLSNPFQ LIFQSGDSTQ
     ANYFRIPTLY TLSSGRVLSS IDARYGGTHD SKSKINIATS YSDDNGKTWS EPIFAMKFND
     YEEQLVYWPR DNKLKNSQIS GSASFIDSSI VEDKKSGKTI LLADVMPAGI GNNNANKADS
     GFKEINGHYY LKLKKNGDND FRYTVRENGV VYNETTNKPT NYTINDKYEV LEGGKSLTVE
     QYSVDFDSGS LRERHNGKQV PMNVFYKDSL FKVTPTNYIA MTTSQNRGES WEQFKLLPPF
     LGEKHNGTYL CPGQGLALKS SNRLIFATYT SGELTYLISD DSGQTWKKSS ASIPFKNATA
     EAQMVELRDG VIRTFFRTTT GKIAYMTSRD SGETWSKVSY IDGIQQTSYG TQVSAIKYSQ
     LIDGKEAVIL STPNSRSGRK GGQLVVGLVN KEDDSIDWKY HYDIDLPSYG YAYSAITELP
     NHHIGVLFEK YDSWSRNELH LSNVVQYIDL EINDLTK
//
DBGET integrated database retrieval system