ID NIFD_METMP Reviewed; 477 AA.
AC P0CW51; P71526;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 03-MAY-2011, sequence version 1.
DT 01-MAY-2013, entry version 13.
DE RecName: Full=Nitrogenase molybdenum-iron protein alpha chain;
DE EC=1.18.6.1;
DE AltName: Full=Dinitrogenase;
DE AltName: Full=Nitrogenase component I;
GN Name=nifD; OrderedLocusNames=MMP0856;
OS Methanococcus maripaludis (strain S2 / LL).
OC Archaea; Euryarchaeota; Methanococci; Methanococcales;
OC Methanococcaceae; Methanococcus.
OX NCBI_TaxID=267377;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S2 / LL;
RX PubMed=15466049; DOI=10.1128/JB.186.20.6956-6969.2004;
RA Hendrickson E.L., Kaul R., Zhou Y., Bovee D., Chapman P., Chung J.,
RA Conway de Macario E., Dodsworth J.A., Gillett W., Graham D.E.,
RA Hackett M., Haydock A.K., Kang A., Land M.L., Levy R., Lie T.J.,
RA Major T.A., Moore B.C., Porat I., Palmeiri A., Rouse G.,
RA Saenphimmachak C., Soell D., Van Dien S., Wang T., Whitman W.B.,
RA Xia Q., Zhang Y., Larimer F.W., Olson M.V., Leigh J.A.;
RT "Complete genome sequence of the genetically tractable
RT hydrogenotrophic methanogen Methanococcus maripaludis.";
RL J. Bacteriol. 186:6956-6969(2004).
CC -!- FUNCTION: This molybdenum-iron protein is part of the nitrogenase
CC complex that catalyzes the key enzymatic reactions in nitrogen
CC fixation (By similarity).
CC -!- CATALYTIC ACTIVITY: 8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP
CC + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16
CC phosphate.
CC -!- COFACTOR: Binds 1 8Fe-7S cluster per heterodimer (By similarity).
CC -!- COFACTOR: Binds 1 7Fe-Mo-9S-X-homocitryl cluster per subunit. The
CC identity of the X atom is not known, possibly carbon or oxygen (By
CC similarity).
CC -!- SUBUNIT: Tetramer of two alpha and two beta chains. Forms complex
CC with the iron protein (nitrogenase component 2) (By similarity).
CC -!- SIMILARITY: Belongs to the NifD/NifK/NifE/NifN family.
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DR EMBL; BX950229; CAF30412.1; -; Genomic_DNA.
DR RefSeq; NP_987976.1; NC_005791.1.
DR ProteinModelPortal; P0CW51; -.
DR EnsemblBacteria; CAF30412; CAF30412; MMP0856.
DR GeneID; 2761670; -.
DR KEGG; mmp:MMP0856; -.
DR HOGENOM; HOG000005528; -.
DR KO; K02586; -.
DR OMA; KDVIHIS; -.
DR BioCyc; MMAR267377:GJ77-886-MONOMER; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0018697; F:carbonyl sulfide nitrogenase activity; IEA:EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016163; F:nitrogenase activity; IEA:EC.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR InterPro; IPR000510; Nase/OxRdtase_comp1.
DR InterPro; IPR005974; Nase_asu.
DR InterPro; IPR010143; Nase_comp1_asu.
DR InterPro; IPR000318; Nase_comp1_CS.
DR Pfam; PF00148; Oxidored_nitro; 1.
DR TIGRFAMs; TIGR01284; alt_nitrog_alph; 1.
DR TIGRFAMs; TIGR01862; N2-ase-Ialpha; 1.
DR PROSITE; PS00699; NITROGENASE_1_1; FALSE_NEG.
DR PROSITE; PS00090; NITROGENASE_1_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Complete proteome; Iron; Iron-sulfur; Metal-binding;
KW Molybdenum; Nitrogen fixation; Nucleotide-binding; Oxidoreductase.
FT CHAIN 1 477 Nitrogenase molybdenum-iron protein alpha
FT chain.
FT /FTId=PRO_0000408202.
FT METAL 49 49 Iron-sulfur (8Fe-7S); shared with beta
FT chain (By similarity).
FT METAL 75 75 Iron-sulfur (8Fe-7S); shared with beta
FT chain (By similarity).
FT METAL 149 149 Iron-sulfur (8Fe-7S); shared with beta
FT chain (By similarity).
FT METAL 267 267 Molybdenum-iron-sulfur (7Fe-Mo-9S-X-
FT homocitryl) (By similarity).
FT METAL 433 433 Molybdenum-iron-sulfur (7Fe-Mo-9S-X-
FT homocitryl); via pros nitrogen (By
FT similarity).
SQ SEQUENCE 477 AA; 53632 MW; 2773022686D9440C CRC64;
MPFCLLDVDK DIPEREQHIY IKDSKEPKGH CKQRCNTNTI PGSMTERGCA FAGVKGVITG
AIKDVLHVVH SPVGCTAYGN GTTKRYPTRP EMPDGSVFPV ENFNLKHIVG TDLTESDVVF
GGMNKLKKVI REASKEFPFV NAIYVYATCT TGLIGDDLDA VCKEMQAELG KDVVAFNAPG
FAGPTQSKGH HVGNYTIFEK LVGTKEPPKT TDYDINLIGE YNIDGDYWVL EKYFEDMGIN
VLSKFTGDAT HGELCWMHKA KLSLVRCQRS ATYVAKLIEE KYGVPYLKVD FFGPEYCAEN
LRAVGKYFGK EIEAEAVIKK EMEKIQPELD FYKSKLQGKK IWISAGGPKS WHLSKPIEQY
LGMDVVALSG LFEHEDGYEK MQERAKDGTI IIDDPNTLEM EEVVEKYQPE IVLGGIKEKY
FFHKLGVPSV MIHSYENGPY IGFEGFVNMA RDIYTAIYNP AWKLMEFEEE EPGDSNE
//
