ID NIFD_METTH Reviewed; 469 AA.
AC O27605;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 01-MAY-2013, entry version 87.
DE RecName: Full=Nitrogenase molybdenum-iron protein alpha chain;
DE EC=1.18.6.1;
DE AltName: Full=Dinitrogenase;
DE AltName: Full=Nitrogenase component I;
GN Name=nifD; OrderedLocusNames=MTH_1563;
OS Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 /
OS JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium
OS thermoautotrophicum).
OC Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales;
OC Methanobacteriaceae; Methanothermobacter.
OX NCBI_TaxID=187420;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=9371463;
RA Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K.,
RA Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D.,
RA Spadafora R., Vicare R., Wang Y., Wierzbowski J., Gibson R.,
RA Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S.,
RA McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M.,
RA Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.;
RT "Complete genome sequence of Methanobacterium thermoautotrophicum
RT deltaH: functional analysis and comparative genomics.";
RL J. Bacteriol. 179:7135-7155(1997).
CC -!- FUNCTION: This molybdenum-iron protein is part of the nitrogenase
CC complex that catalyzes the key enzymatic reactions in nitrogen
CC fixation.
CC -!- CATALYTIC ACTIVITY: 8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP
CC + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16
CC phosphate.
CC -!- COFACTOR: Binds 1 8Fe-7S cluster per heterodimer (By similarity).
CC -!- COFACTOR: Binds 1 7Fe-Mo-9S-X-homocitryl cluster per subunit. The
CC identity of the X atom is not known, possibly carbon or oxygen (By
CC similarity).
CC -!- SUBUNIT: Tetramer of two alpha and two beta chains. Forms complex
CC with the iron protein (nitrogenase component 2).
CC -!- SIMILARITY: Belongs to the NifD/NifK/NifE/NifN family.
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DR EMBL; AE000666; AAB86037.1; -; Genomic_DNA.
DR PIR; F69075; F69075.
DR RefSeq; NP_276676.1; NC_000916.1.
DR ProteinModelPortal; O27605; -.
DR STRING; 187420.MTH1563; -.
DR EnsemblBacteria; AAB86037; AAB86037; MTH_1563.
DR GeneID; 1471832; -.
DR KEGG; mth:MTH1563; -.
DR eggNOG; COG2710; -.
DR KO; K02586; -.
DR OMA; KDVIHIS; -.
DR ProtClustDB; CLSK878514; -.
DR BioCyc; MTHE187420:GJNM-1565-MONOMER; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0018697; F:carbonyl sulfide nitrogenase activity; IEA:EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016163; F:nitrogenase activity; IEA:EC.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR InterPro; IPR000510; Nase/OxRdtase_comp1.
DR InterPro; IPR005974; Nase_asu.
DR InterPro; IPR010143; Nase_comp1_asu.
DR InterPro; IPR000318; Nase_comp1_CS.
DR Pfam; PF00148; Oxidored_nitro; 1.
DR TIGRFAMs; TIGR01284; alt_nitrog_alph; 1.
DR TIGRFAMs; TIGR01862; N2-ase-Ialpha; 1.
DR PROSITE; PS00699; NITROGENASE_1_1; FALSE_NEG.
DR PROSITE; PS00090; NITROGENASE_1_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Complete proteome; Iron; Iron-sulfur; Metal-binding;
KW Molybdenum; Nitrogen fixation; Nucleotide-binding; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1 469 Nitrogenase molybdenum-iron protein alpha
FT chain.
FT /FTId=PRO_0000153073.
FT METAL 48 48 Iron-sulfur (8Fe-7S); shared with beta
FT chain (By similarity).
FT METAL 74 74 Iron-sulfur (8Fe-7S); shared with beta
FT chain (By similarity).
FT METAL 147 147 Iron-sulfur (8Fe-7S); shared with beta
FT chain (By similarity).
FT METAL 265 265 Molybdenum-iron-sulfur (7Fe-Mo-9S-X-
FT homocitryl) (By similarity).
FT METAL 431 431 Molybdenum-iron-sulfur (7Fe-Mo-9S-X-
FT homocitryl); via pros nitrogen (By
FT similarity).
SQ SEQUENCE 469 AA; 52866 MW; 7D2CD5FFED9EC7B8 CRC64;
MPFKLFDVDA EIPERKKHVY IKKKEDPEED LPLCNTKTIP GCMTERGCAF AGAKGVITGA
IKDALHVIHS PVGCTAYGYG TKRYPTSQEM PDGSLFPIEN FNLKYITGTN LTETDVVFGG
MDKLKRCIIE AAKEFPEANA VYTYATCTTG LIGDDIEAVS REVSEEIGKD VVAINAPGFA
GPTQSKGHQV ANYTLFENLV GTAEPPVVTD YDVNLIGEYN IDGDLWVLKR YFEEMGINVL
STFTGDCCHD EIKWMHRAKL SLVRCQRSAT YIARLLEERY NVPYMKVDFF GIEYCKRNLM
ATGEYFGIPE RAEEVIKDRM EKIGPEIEYL RNKLAGKKVW VFSGGPKNWH LPRPLEDELG
MEVVAVSTMF EHEDGYEKIK KRVGENTVIV DDPNSLELEE IIEKYRPDII LSGIKEKYLA
HKLGVPCVLI HSYENGPYIG FEGFLNLARD MYASIYSPVW DLLEFEAGD
//
