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Entry: PHHY_PSEAE
LinkDB: PHHY_PSEAE
Original site: PHHY_PSEAE 
ID   PHHY_PSEAE              Reviewed;         394 AA.
AC   P20586;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   26-NOV-2014, entry version 110.
DE   RecName: Full=p-hydroxybenzoate hydroxylase;
DE            Short=PHBH;
DE            EC=1.14.13.2;
DE   AltName: Full=4-hydroxybenzoate 3-monooxygenase;
GN   Name=pobA; OrderedLocusNames=PA0247;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG
OS   12228).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2465205; DOI=10.1016/0378-1119(88)90044-3;
RA   Entsch B., Nan Y., Weaich K., Scott K.F.;
RT   "Sequence and organization of pobA, the gene coding for p-
RT   hydroxybenzoate hydroxylase, an inducible enzyme from Pseudomonas
RT   aeruginosa.";
RL   Gene 71:279-291(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T.,
RA   Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an
RT   opportunistic pathogen.";
RL   Nature 406:959-964(2000).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
RX   PubMed=7939628; DOI=10.1126/science.7939628;
RA   Gatti D.L., Palfey B.A., Lah M.S., Entsch B., Massey V., Ballou D.P.,
RA   Ludwig M.L.;
RT   "The mobile flavin of 4-OH benzoate hydroxylase.";
RL   Science 266:110-114(1994).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
RX   PubMed=8555229; DOI=10.1021/bi951344i;
RA   Gatti D.L., Entsch B., Ballou D.P., Ludwig M.L.;
RT   "pH-dependent structural changes in the active site of p-
RT   hydroxybenzoate hydroxylase point to the importance of proton and
RT   water movements during catalysis.";
RL   Biochemistry 35:567-578(1996).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF MUTANTS.
RX   PubMed=8312276; DOI=10.1021/bi00172a036;
RA   Lah M.S., Palfey B.A., Schreuder H.A., Ludwig M.L.;
RT   "Crystal structures of mutant Pseudomonas aeruginosa p-hydroxybenzoate
RT   hydroxylases: the Tyr201Phe, Tyr385Phe, and Asn300Asp variants.";
RL   Biochemistry 33:1555-1564(1994).
CC   -!- CATALYTIC ACTIVITY: 4-hydroxybenzoate + NADPH + O(2) =
CC       protocatechuate + NADP(+) + H(2)O.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC   -!- PATHWAY: Aromatic compound metabolism; benzoate degradation via
CC       hydroxylation; 3,4-dihydroxybenzoate from benzoate: step 2/2.
CC   -!- SUBUNIT: Homodimer.
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DR   EMBL; M23173; AAA88455.1; -; Genomic_DNA.
DR   EMBL; AE004091; AAG03636.1; -; Genomic_DNA.
DR   PIR; JT0384; WHPSBA.
DR   RefSeq; NP_248938.1; NC_002516.2.
DR   PDB; 1D7L; X-ray; 2.20 A; A=1-394.
DR   PDB; 1DOB; X-ray; 2.00 A; A=1-394.
DR   PDB; 1DOC; X-ray; 2.00 A; A=1-394.
DR   PDB; 1DOD; X-ray; 2.10 A; A=1-394.
DR   PDB; 1DOE; X-ray; 2.30 A; A=1-394.
DR   PDB; 1IUS; X-ray; 2.20 A; A=1-394.
DR   PDB; 1IUT; X-ray; 2.00 A; A=1-394.
DR   PDB; 1IUU; X-ray; 2.00 A; A=1-394.
DR   PDB; 1IUV; X-ray; 2.50 A; A=1-394.
DR   PDB; 1IUW; X-ray; 2.00 A; A=1-394.
DR   PDB; 1IUX; X-ray; 2.00 A; A=1-394.
DR   PDB; 1K0I; X-ray; 1.80 A; A=1-394.
DR   PDB; 1K0J; X-ray; 2.20 A; A=1-394.
DR   PDB; 1K0L; X-ray; 2.00 A; A=1-394.
DR   PDB; 1PXA; X-ray; 2.30 A; A=1-394.
DR   PDB; 1PXB; X-ray; 2.30 A; A=1-394.
DR   PDB; 1PXC; X-ray; 2.10 A; A=1-394.
DR   PDB; 1YKJ; X-ray; 2.00 A; A/B=1-394.
DR   PDBsum; 1D7L; -.
DR   PDBsum; 1DOB; -.
DR   PDBsum; 1DOC; -.
DR   PDBsum; 1DOD; -.
DR   PDBsum; 1DOE; -.
DR   PDBsum; 1IUS; -.
DR   PDBsum; 1IUT; -.
DR   PDBsum; 1IUU; -.
DR   PDBsum; 1IUV; -.
DR   PDBsum; 1IUW; -.
DR   PDBsum; 1IUX; -.
DR   PDBsum; 1K0I; -.
DR   PDBsum; 1K0J; -.
DR   PDBsum; 1K0L; -.
DR   PDBsum; 1PXA; -.
DR   PDBsum; 1PXB; -.
DR   PDBsum; 1PXC; -.
DR   PDBsum; 1YKJ; -.
DR   ProteinModelPortal; P20586; -.
DR   SMR; P20586; 1-394.
DR   STRING; 208964.PA0247; -.
DR   DrugBank; DB03147; Flavin adenine dinucleotide.
DR   EnsemblBacteria; AAG03636; AAG03636; PA0247.
DR   GeneID; 882128; -.
DR   KEGG; pae:PA0247; -.
DR   PATRIC; 19834710; VBIPseAer58763_0257.
DR   PseudoCAP; PA0247; -.
DR   eggNOG; COG0654; -.
DR   HOGENOM; HOG000221233; -.
DR   InParanoid; P20586; -.
DR   KO; K00481; -.
DR   OMA; LAEWSDA; -.
DR   OrthoDB; EOG6PS5RW; -.
DR   PhylomeDB; P20586; -.
DR   UniPathway; UPA00156; UER00257.
DR   EvolutionaryTrace; P20586; -.
DR   GO; GO:0018659; F:4-hydroxybenzoate 3-monooxygenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0043640; P:benzoate catabolic process via hydroxylation; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR012733; HB_mOase.
DR   InterPro; IPR002938; mOase_FAD-bd.
DR   InterPro; IPR003042; Rng_hydrolase-like.
DR   Pfam; PF01494; FAD_binding_3; 1.
DR   PRINTS; PR00420; RNGMNOXGNASE.
DR   TIGRFAMs; TIGR02360; pbenz_hydroxyl; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Aromatic hydrocarbons catabolism; Complete proteome;
KW   FAD; Flavoprotein; Monooxygenase; NADP; Oxidoreductase;
KW   Reference proteome.
FT   CHAIN         1    394       p-hydroxybenzoate hydroxylase.
FT                                /FTId=PRO_0000058381.
FT   NP_BIND       4     32       FAD. {ECO:0000255}.
FT   STRAND        4      8       {ECO:0000244|PDB:1K0I}.
FT   HELIX        12     24       {ECO:0000244|PDB:1K0I}.
FT   STRAND       28     31       {ECO:0000244|PDB:1K0I}.
FT   HELIX        36     40       {ECO:0000244|PDB:1K0I}.
FT   STRAND       47     49       {ECO:0000244|PDB:1DOB}.
FT   HELIX        50     58       {ECO:0000244|PDB:1K0I}.
FT   HELIX        63     68       {ECO:0000244|PDB:1K0I}.
FT   STRAND       70     73       {ECO:0000244|PDB:1K0I}.
FT   STRAND       75     79       {ECO:0000244|PDB:1K0I}.
FT   STRAND       82     86       {ECO:0000244|PDB:1K0I}.
FT   HELIX        88     92       {ECO:0000244|PDB:1K0I}.
FT   STRAND       97     99       {ECO:0000244|PDB:1K0I}.
FT   HELIX       102    115       {ECO:0000244|PDB:1K0I}.
FT   STRAND      119    123       {ECO:0000244|PDB:1K0I}.
FT   STRAND      125    130       {ECO:0000244|PDB:1K0I}.
FT   STRAND      134    136       {ECO:0000244|PDB:1K0I}.
FT   STRAND      138    143       {ECO:0000244|PDB:1K0I}.
FT   STRAND      146    151       {ECO:0000244|PDB:1K0I}.
FT   STRAND      153    157       {ECO:0000244|PDB:1K0I}.
FT   HELIX       166    168       {ECO:0000244|PDB:1K0I}.
FT   HELIX       171    173       {ECO:0000244|PDB:1K0I}.
FT   STRAND      175    192       {ECO:0000244|PDB:1K0I}.
FT   STRAND      195    198       {ECO:0000244|PDB:1DOB}.
FT   STRAND      200    202       {ECO:0000244|PDB:1K0I}.
FT   STRAND      209    215       {ECO:0000244|PDB:1K0I}.
FT   STRAND      218    225       {ECO:0000244|PDB:1K0I}.
FT   HELIX       231    233       {ECO:0000244|PDB:1K0I}.
FT   HELIX       236    245       {ECO:0000244|PDB:1K0I}.
FT   HELIX       249    254       {ECO:0000244|PDB:1K0I}.
FT   STRAND      260    274       {ECO:0000244|PDB:1K0I}.
FT   STRAND      276    278       {ECO:0000244|PDB:1K0I}.
FT   STRAND      281    283       {ECO:0000244|PDB:1K0I}.
FT   HELIX       285    287       {ECO:0000244|PDB:1K0I}.
FT   HELIX       293    295       {ECO:0000244|PDB:1K0I}.
FT   HELIX       298    319       {ECO:0000244|PDB:1K0I}.
FT   HELIX       322    327       {ECO:0000244|PDB:1K0I}.
FT   HELIX       328    350       {ECO:0000244|PDB:1K0I}.
FT   HELIX       358    373       {ECO:0000244|PDB:1K0I}.
FT   HELIX       375    385       {ECO:0000244|PDB:1K0I}.
SQ   SEQUENCE   394 AA;  44324 MW;  1E7232854D9EC792 CRC64;
     MKTQVAIIGA GPSGLLLGQL LHKAGIDNVI LERQTPDYVL GRIRAGVLEQ GMVDLLREAG
     VDRRMARDGL VHEGVEIAFA GQRRRIDLKR LSGGKTVTVY GQTEVTRDLM EAREACGATT
     VYQAAEVRLH DLQGERPYVT FERDGERLRL DCDYIAGCDG FHGISRQSIP AERLKVFERV
     YPFGWLGLLA DTPPVSHELI YANHPRGFAL CSQRSATRSR YYVQVPLSEK VEDWSDERFW
     TELKARLPSE VAEKLVTGPS LEKSIAPLRS FVVEPMQHGR LFLAGDAAHI VPPTGAKGLN
     LAASDVSTLY RLLLKAYREG RGELLERYSA ICLRRIWKAE RFSWWMTSVL HRFPDTDAFS
     QRIQQTELEY YLGSEAGLAT IAENYVGLPY EEIE
//
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