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Entry: PHHY_PSEAE
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Original site: PHHY_PSEAE 
ID   PHHY_PSEAE              Reviewed;         394 AA.
AC   P20586;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   09-JUL-2014, entry version 107.
DE   RecName: Full=p-hydroxybenzoate hydroxylase;
DE            Short=PHBH;
DE            EC=1.14.13.2;
DE   AltName: Full=4-hydroxybenzoate 3-monooxygenase;
GN   Name=pobA; OrderedLocusNames=PA0247;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG
OS   12228).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2465205; DOI=10.1016/0378-1119(88)90044-3;
RA   Entsch B., Nan Y., Weaich K., Scott K.F.;
RT   "Sequence and organization of pobA, the gene coding for p-
RT   hydroxybenzoate hydroxylase, an inducible enzyme from Pseudomonas
RT   aeruginosa.";
RL   Gene 71:279-291(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T.,
RA   Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an
RT   opportunistic pathogen.";
RL   Nature 406:959-964(2000).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
RX   PubMed=7939628; DOI=10.1126/science.7939628;
RA   Gatti D.L., Palfey B.A., Lah M.S., Entsch B., Massey V., Ballou D.P.,
RA   Ludwig M.L.;
RT   "The mobile flavin of 4-OH benzoate hydroxylase.";
RL   Science 266:110-114(1994).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
RX   PubMed=8555229; DOI=10.1021/bi951344i;
RA   Gatti D.L., Entsch B., Ballou D.P., Ludwig M.L.;
RT   "pH-dependent structural changes in the active site of p-
RT   hydroxybenzoate hydroxylase point to the importance of proton and
RT   water movements during catalysis.";
RL   Biochemistry 35:567-578(1996).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF MUTANTS.
RX   PubMed=8312276; DOI=10.1021/bi00172a036;
RA   Lah M.S., Palfey B.A., Schreuder H.A., Ludwig M.L.;
RT   "Crystal structures of mutant Pseudomonas aeruginosa p-hydroxybenzoate
RT   hydroxylases: the Tyr201Phe, Tyr385Phe, and Asn300Asp variants.";
RL   Biochemistry 33:1555-1564(1994).
CC   -!- CATALYTIC ACTIVITY: 4-hydroxybenzoate + NADPH + O(2) =
CC       protocatechuate + NADP(+) + H(2)O.
CC   -!- COFACTOR: FAD.
CC   -!- PATHWAY: Aromatic compound metabolism; benzoate degradation via
CC       hydroxylation; 3,4-dihydroxybenzoate from benzoate: step 2/2.
CC   -!- SUBUNIT: Homodimer.
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DR   EMBL; M23173; AAA88455.1; -; Genomic_DNA.
DR   EMBL; AE004091; AAG03636.1; -; Genomic_DNA.
DR   PIR; JT0384; WHPSBA.
DR   RefSeq; NP_248938.1; NC_002516.2.
DR   PDB; 1D7L; X-ray; 2.20 A; A=1-394.
DR   PDB; 1DOB; X-ray; 2.00 A; A=1-394.
DR   PDB; 1DOC; X-ray; 2.00 A; A=1-394.
DR   PDB; 1DOD; X-ray; 2.10 A; A=1-394.
DR   PDB; 1DOE; X-ray; 2.30 A; A=1-394.
DR   PDB; 1IUS; X-ray; 2.20 A; A=1-394.
DR   PDB; 1IUT; X-ray; 2.00 A; A=1-394.
DR   PDB; 1IUU; X-ray; 2.00 A; A=1-394.
DR   PDB; 1IUV; X-ray; 2.50 A; A=1-394.
DR   PDB; 1IUW; X-ray; 2.00 A; A=1-394.
DR   PDB; 1IUX; X-ray; 2.00 A; A=1-394.
DR   PDB; 1K0I; X-ray; 1.80 A; A=1-394.
DR   PDB; 1K0J; X-ray; 2.20 A; A=1-394.
DR   PDB; 1K0L; X-ray; 2.00 A; A=1-394.
DR   PDB; 1PXA; X-ray; 2.30 A; A=1-394.
DR   PDB; 1PXB; X-ray; 2.30 A; A=1-394.
DR   PDB; 1PXC; X-ray; 2.10 A; A=1-394.
DR   PDB; 1YKJ; X-ray; 2.00 A; A/B=1-394.
DR   PDBsum; 1D7L; -.
DR   PDBsum; 1DOB; -.
DR   PDBsum; 1DOC; -.
DR   PDBsum; 1DOD; -.
DR   PDBsum; 1DOE; -.
DR   PDBsum; 1IUS; -.
DR   PDBsum; 1IUT; -.
DR   PDBsum; 1IUU; -.
DR   PDBsum; 1IUV; -.
DR   PDBsum; 1IUW; -.
DR   PDBsum; 1IUX; -.
DR   PDBsum; 1K0I; -.
DR   PDBsum; 1K0J; -.
DR   PDBsum; 1K0L; -.
DR   PDBsum; 1PXA; -.
DR   PDBsum; 1PXB; -.
DR   PDBsum; 1PXC; -.
DR   PDBsum; 1YKJ; -.
DR   ProteinModelPortal; P20586; -.
DR   SMR; P20586; 1-394.
DR   STRING; 208964.PA0247; -.
DR   EnsemblBacteria; AAG03636; AAG03636; PA0247.
DR   GeneID; 882128; -.
DR   KEGG; pae:PA0247; -.
DR   PATRIC; 19834710; VBIPseAer58763_0257.
DR   PseudoCAP; PA0247; -.
DR   eggNOG; COG0654; -.
DR   HOGENOM; HOG000221233; -.
DR   KO; K00481; -.
DR   OMA; LAEWSDA; -.
DR   OrthoDB; EOG6PS5RW; -.
DR   PhylomeDB; P20586; -.
DR   UniPathway; UPA00156; UER00257.
DR   EvolutionaryTrace; P20586; -.
DR   GO; GO:0018659; F:4-hydroxybenzoate 3-monooxygenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0043640; P:benzoate catabolic process via hydroxylation; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR012733; HB_mOase.
DR   InterPro; IPR002938; mOase_FAD-bd.
DR   InterPro; IPR003042; Rng_hydrolase-like.
DR   Pfam; PF01494; FAD_binding_3; 1.
DR   PRINTS; PR00420; RNGMNOXGNASE.
DR   TIGRFAMs; TIGR02360; pbenz_hydroxyl; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Aromatic hydrocarbons catabolism; Complete proteome;
KW   FAD; Flavoprotein; Monooxygenase; NADP; Oxidoreductase;
KW   Reference proteome.
FT   CHAIN         1    394       p-hydroxybenzoate hydroxylase.
FT                                /FTId=PRO_0000058381.
FT   NP_BIND       4     32       FAD (Potential).
FT   STRAND        4      8
FT   HELIX        12     24
FT   STRAND       28     31
FT   HELIX        36     40
FT   STRAND       47     49
FT   HELIX        50     58
FT   HELIX        63     68
FT   STRAND       70     73
FT   STRAND       75     79
FT   STRAND       82     86
FT   HELIX        88     92
FT   STRAND       97     99
FT   HELIX       102    115
FT   STRAND      119    123
FT   STRAND      125    130
FT   STRAND      134    136
FT   STRAND      138    143
FT   STRAND      146    151
FT   STRAND      153    157
FT   HELIX       166    168
FT   HELIX       171    173
FT   STRAND      175    192
FT   STRAND      195    198
FT   STRAND      200    202
FT   STRAND      209    215
FT   STRAND      218    225
FT   HELIX       231    233
FT   HELIX       236    245
FT   HELIX       249    254
FT   STRAND      260    274
FT   STRAND      276    278
FT   STRAND      281    283
FT   HELIX       285    287
FT   HELIX       293    295
FT   HELIX       298    319
FT   HELIX       322    327
FT   HELIX       328    350
FT   HELIX       358    373
FT   HELIX       375    385
SQ   SEQUENCE   394 AA;  44324 MW;  1E7232854D9EC792 CRC64;
     MKTQVAIIGA GPSGLLLGQL LHKAGIDNVI LERQTPDYVL GRIRAGVLEQ GMVDLLREAG
     VDRRMARDGL VHEGVEIAFA GQRRRIDLKR LSGGKTVTVY GQTEVTRDLM EAREACGATT
     VYQAAEVRLH DLQGERPYVT FERDGERLRL DCDYIAGCDG FHGISRQSIP AERLKVFERV
     YPFGWLGLLA DTPPVSHELI YANHPRGFAL CSQRSATRSR YYVQVPLSEK VEDWSDERFW
     TELKARLPSE VAEKLVTGPS LEKSIAPLRS FVVEPMQHGR LFLAGDAAHI VPPTGAKGLN
     LAASDVSTLY RLLLKAYREG RGELLERYSA ICLRRIWKAE RFSWWMTSVL HRFPDTDAFS
     QRIQQTELEY YLGSEAGLAT IAENYVGLPY EEIE
//
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