ID PTFBC_ECOLI Reviewed; 563 AA.
AC P20966;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 01-MAY-2013, entry version 117.
DE RecName: Full=PTS system fructose-specific EIIBC component;
DE AltName: Full=EIIBC-Fru;
DE Includes:
DE RecName: Full=Fructose-specific phosphotransferase enzyme IIB component;
DE EC=2.7.1.69;
DE AltName: Full=EIII-Fru;
DE AltName: Full=PTS system fructose-specific EIIB component;
DE Includes:
DE RecName: Full=Fructose permease IIC component;
DE AltName: Full=PTS system fructose-specific EIIC component;
GN Name=fruA; Synonyms=ptsF; OrderedLocusNames=b2167, JW2154;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=3076173;
RA Prior T.I., Kornberg H.L.;
RT "Nucleotide sequence of fruA, the gene specifying enzyme IIfru of the
RT phosphoenolpyruvate-dependent sugar phosphotransferase system in
RT Escherichia coli K12.";
RL J. Gen. Microbiol. 134:2757-2768(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / BHB2600;
RA Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K.,
RA Church G.M.;
RT "Automated multiplex sequencing of the E.coli genome.";
RL Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains
RT MG1655 and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-365.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C.,
RA Yamamoto Y., Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome
RT corresponding to the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-74.
RC STRAIN=K12;
RX PubMed=1981619;
RA Orchard L.M.D., Kornberg H.L.;
RT "Sequence similarities between the gene specifying 1-
RT phosphofructokinase (fruK), genes specifying other kinases in
RT Escherichia coli K12, and lacC of Staphylococcus aureus.";
RL Proc. R. Soc. B 242:87-90(1990).
RN [7]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: The phosphoenolpyruvate-dependent sugar
CC phosphotransferase system (sugar PTS), a major carbohydrate active
CC -transport system, catalyzes the phosphorylation of incoming sugar
CC substrates concomitantly with their translocation across the cell
CC membrane. This system is involved in fructose transport.
CC -!- CATALYTIC ACTIVITY: Protein EIIB N(pi)-phospho-L-
CC histidine/cysteine + sugar = protein EIIB + sugar phosphate.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane
CC protein.
CC -!- DOMAIN: The EIIB domain is phosphorylated by phospho-EIIA on a
CC cysteinyl or histidyl residue, depending on the transported sugar.
CC Then, it transfers the phosphoryl group to the sugar substrate
CC concomitantly with the sugar uptake processed by the EIIC domain.
CC -!- DOMAIN: The EIIC domain forms the PTS system translocation channel
CC and contains the specific substrate-binding site.
CC -!- SIMILARITY: Contains 1 PTS EIIB type-2 domain.
CC -!- SIMILARITY: Contains 1 PTS EIIC type-2 domain.
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DR EMBL; M23196; AAA62624.1; -; Genomic_DNA.
DR EMBL; U00007; AAA60524.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75228.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15976.2; -; Genomic_DNA.
DR EMBL; X53948; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; A34962; A34962.
DR RefSeq; NP_416672.1; NC_000913.2.
DR RefSeq; YP_490406.1; NC_007779.1.
DR ProteinModelPortal; P20966; -.
DR SMR; P20966; 1-86, 105-205.
DR IntAct; P20966; 1.
DR STRING; 511145.b2167; -.
DR TCDB; 4.A.2.1.1; PTS fructose-mannitol (Fru) family.
DR PaxDb; P20966; -.
DR PRIDE; P20966; -.
DR EnsemblBacteria; AAC75228; AAC75228; b2167.
DR EnsemblBacteria; BAA15976; BAA15976; BAA15976.
DR GeneID; 12931469; -.
DR GeneID; 946672; -.
DR KEGG; ecj:Y75_p2129; -.
DR KEGG; eco:b2167; -.
DR PATRIC; 32119683; VBIEscCol129921_2252.
DR EchoBASE; EB0332; -.
DR EcoGene; EG10336; fruA.
DR eggNOG; COG1445; -.
DR HOGENOM; HOG000227677; -.
DR KO; K02769; -.
DR KO; K02770; -.
DR OMA; INHALLY; -.
DR ProtClustDB; PRK10712; -.
DR BioCyc; EcoCyc:FRUA-MONOMER; -.
DR BioCyc; ECOL316407:JW2154-MONOMER; -.
DR Genevestigator; P20966; -.
DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:EC.
DR GO; GO:0005351; F:sugar:hydrogen symporter activity; IEA:InterPro.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR InterPro; IPR013011; PTS_EIIB_2.
DR InterPro; IPR003501; PTS_EIIB_2/3.
DR InterPro; IPR003352; PTS_EIIC.
DR InterPro; IPR013014; PTS_EIIC_2.
DR InterPro; IPR003353; PTS_IIB_fruc.
DR InterPro; IPR006327; PTS_IIC_fruc.
DR Pfam; PF02378; PTS_EIIC; 1.
DR Pfam; PF02302; PTS_IIB; 1.
DR TIGRFAMs; TIGR00829; FRU; 1.
DR TIGRFAMs; TIGR01427; PTS_IIC_fructo; 1.
DR PROSITE; PS51099; PTS_EIIB_TYPE_2; 1.
DR PROSITE; PS51104; PTS_EIIC_TYPE_2; 1.
PE 4: Predicted;
KW Cell inner membrane; Cell membrane; Complete proteome; Kinase;
KW Membrane; Phosphotransferase system; Reference proteome; Repeat;
KW Sugar transport; Transferase; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1 563 PTS system fructose-specific EIIBC
FT component.
FT /FTId=PRO_0000186508.
FT TRANSMEM 236 256 Helical; (Potential).
FT TRANSMEM 274 294 Helical; (Potential).
FT TRANSMEM 304 324 Helical; (Potential).
FT TRANSMEM 349 369 Helical; (Potential).
FT TRANSMEM 382 402 Helical; (Potential).
FT TRANSMEM 430 450 Helical; (Potential).
FT TRANSMEM 463 483 Helical; (Potential).
FT TRANSMEM 489 509 Helical; (Potential).
FT TRANSMEM 518 538 Helical; (Potential).
FT DOMAIN 104 201 PTS EIIB type-2.
FT DOMAIN 226 561 PTS EIIC type-2.
FT ACT_SITE 112 112 Phosphocysteine intermediate; for EIIB
FT activity (By similarity).
SQ SEQUENCE 563 AA; 57519 MW; AC066A96A94DBA05 CRC64;
MKTLLIIDAN LGQARAYMAK TLLGAAARKA KLEIIDNPND AEMAIVLGDS IPNDSALNGK
NVWLGDISRA VAHPELFLSE AKGHAKPYTA PVAATAPVAA SGPKRVVAVT ACPTGVAHTF
MAAEAIETEA KKRGWWVKVE TRGSVGAGNA ITPEEVAAAD LVIVAADIEV DLAKFAGKPM
YRTSTGLALK KTAQELDKAV AEATPYEPAG KAQTATTESK KESAGAYRHL LTGVSYMLPM
VVAGGLCIAL SFAFGIEAFK EPGTLAAALM QIGGGSAFAL MVPVLAGYIA FSIADRPGLT
PGLIGGMLAV STGSGFIGGI IAGFLAGYIA KLISTQLKLP QSMEALKPIL IIPLISSLVV
GLAMIYLIGK PVAGILEGLT HWLQTMGTAN AVLLGAILGG MMCTDMGGPV NKAAYAFGVG
LLSTQTYGPM AAIMAAGMVP PLAMGLATMV ARRKFDKAQQ EGGKAALVLG LCFISEGAIP
FAARDPMRVL PCCIVGGALT GAISMAIGAK LMAPHGGLFV LLIPGAITPV LGYLVAIIAG
TLVAGLAYAF LKRPEVDAVA KAA
//
