GenomeNet

Database: UniProt/SWISS-PROT
Entry: PYRG_METST
LinkDB: PYRG_METST
Original site: PYRG_METST 
ID   PYRG_METST              Reviewed;         534 AA.
AC   Q2NH50;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 1.
DT   11-JUN-2014, entry version 61.
DE   RecName: Full=CTP synthase;
DE            EC=6.3.4.2;
DE   AltName: Full=CTP synthetase;
DE   AltName: Full=UTP--ammonia ligase;
GN   Name=pyrG; OrderedLocusNames=Msp_0453;
OS   Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 /
OS   MCB-3).
OC   Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales;
OC   Methanobacteriaceae; Methanosphaera.
OX   NCBI_TaxID=339860;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3;
RX   PubMed=16385054; DOI=10.1128/JB.188.2.642-658.2006;
RA   Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H.,
RA   Hedderich R., Gottschalk G., Thauer R.K.;
RT   "The genome sequence of Methanosphaera stadtmanae reveals why this
RT   human intestinal archaeon is restricted to methanol and H2 for methane
RT   formation and ATP synthesis.";
RL   J. Bacteriol. 188:642-658(2006).
CC   -!- FUNCTION: Catalyzes the ATP-dependent amination of UTP to CTP with
CC       either L-glutamine or ammonia as the source of nitrogen (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + UTP + L-glutamine = ADP + phosphate +
CC       CTP + L-glutamate.
CC   -!- ENZYME REGULATION: Allosterically activated by GTP, when glutamine
CC       is the substrate. Inhibited by CTP (By similarity).
CC   -!- PATHWAY: Pyrimidine metabolism; CTP biosynthesis via de novo
CC       pathway; CTP from UDP: step 2/2.
CC   -!- SIMILARITY: Belongs to the CTP synthase family.
CC   -!- SIMILARITY: Contains 1 glutamine amidotransferase type-1 domain.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; CP000102; ABC56853.1; -; Genomic_DNA.
DR   RefSeq; YP_447496.1; NC_007681.1.
DR   ProteinModelPortal; Q2NH50; -.
DR   SMR; Q2NH50; 2-530.
DR   STRING; 339860.Msp_0453; -.
DR   EnsemblBacteria; ABC56853; ABC56853; Msp_0453.
DR   GeneID; 3855104; -.
DR   KEGG; mst:Msp_0453; -.
DR   eggNOG; COG0504; -.
DR   HOGENOM; HOG000077515; -.
DR   KO; K01937; -.
DR   OMA; SVNIKYI; -.
DR   BioCyc; MSTA339860:GJEZ-453-MONOMER; -.
DR   UniPathway; UPA00159; UER00277.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003883; F:CTP synthase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0044210; P:'de novo' CTP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-HAMAP.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   HAMAP; MF_01227; PyrG; 1.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR004468; CTP_synthase.
DR   InterPro; IPR017456; CTP_synthase_N.
DR   InterPro; IPR017926; GATASE.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR11550; PTHR11550; 1.
DR   Pfam; PF06418; CTP_synth_N; 1.
DR   Pfam; PF00117; GATase; 1.
DR   SUPFAM; SSF52317; SSF52317; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00337; PyrG; 1.
DR   PROSITE; PS51273; GATASE_TYPE_1; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Glutamine amidotransferase; Ligase;
KW   Nucleotide-binding; Pyrimidine biosynthesis.
FT   CHAIN         1    534       CTP synthase.
FT                                /FTId=PRO_0000266276.
FT   DOMAIN      294    532       Glutamine amidotransferase type-1.
FT   REGION        1    254       Aminator domain.
FT   ACT_SITE    380    380       Nucleophile (By similarity).
FT   ACT_SITE    505    505       By similarity.
FT   ACT_SITE    507    507       By similarity.
SQ   SEQUENCE   534 AA;  59678 MW;  EA4DF9FCC2996E12 CRC64;
     MSKYIVVTGG VVSSIGKGIT SASIGRILRS YGVNVTAIKI DPYLNWDSGT LNPYQHGEVY
     VTDDGMECDL DLGHYERFLD VELSGKANIT TGKVYSSVIE KERKGEYLGS CVQIIPHITD
     EIKLMIRNVA EKTKAEVVMV EVGGTVGDIE SQPFIEAVRQ LKNEEGHDNC MFVHVTYVPY
     LKAAKEFKTK PTQHSTKELR GLGINPDMIV CRSELSLDAN LKEKIAHFCD VPIEAVINTP
     DAHSIYEVPL IMYSANVGSY ILNRLNIDTA TNKADLYEWS QIVEDLKIET PKVKIAVVGK
     YIELEDAYIS IRESLKHAGA ANKVHVDIDW IKADNDFNID ILKQYDGLLI PGGFGERGIN
     GKIEAVKYAI KNNVPIFGIC LGLHAMSIAI AQLNGYPDAN STEFDENSTC PVIDMMEEQK
     KINNMGGTMR LGAYPCKIKE GTLAYEAYKD TNISERHRHR YEVNNEYRDI LTSAGAIISG
     TSPDDFLVEM IELENHPWFL GCQFHPEFKS RPNKAHPLFK SFIKAAKNKK QNQK
//
DBGET integrated database retrieval system