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Database: UniProt/SWISS-PROT
Entry: RPOC_MYCS2
LinkDB: RPOC_MYCS2
Original site: RPOC_MYCS2 
ID   RPOC_MYCS2              Reviewed;        1317 AA.
AC   A0QS66; I7FY06;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   12-APR-2017, entry version 70.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE            Short=RNAP subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01322};
DE   AltName: Full=RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE   AltName: Full=Transcriptase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
GN   Name=rpoC {ECO:0000255|HAMAP-Rule:MF_01322};
GN   OrderedLocusNames=MSMEG_1368, MSMEI_1329;
OS   Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION BY MASS
RP   SPECTROMETRY [LARGE SCALE ANALYSIS], AND IDENTIFICATION OF N-TERMINUS.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through
RT   orthology and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   FUNCTION, AND SUBUNIT.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=19926651; DOI=10.1099/mic.0.033670-0;
RA   Dey A., Verma A.K., Chatterji D.;
RT   "Role of an RNA polymerase interacting protein, MsRbpA, from
RT   Mycobacterium smegmatis in phenotypic tolerance to rifampicin.";
RL   Microbiology 156:873-883(2010).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription
CC       of DNA into RNA using the four ribonucleoside triphosphates as
CC       substrates. {ECO:0000255|HAMAP-Rule:MF_01322,
CC       ECO:0000269|PubMed:19926651}.
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1). {ECO:0000255|HAMAP-Rule:MF_01322}.
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1
CC       beta' and 1 omega subunit. When a sigma factor is associated with
CC       the core the holoenzyme is formed, which can initiate
CC       transcription. {ECO:0000255|HAMAP-Rule:MF_01322,
CC       ECO:0000269|PubMed:19926651}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01322}.
DR   EMBL; CP000480; ABK71951.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP37802.1; -; Genomic_DNA.
DR   RefSeq; WP_003892756.1; NZ_CP009494.1.
DR   RefSeq; YP_885754.1; NC_008596.1.
DR   PDB; 5TW1; X-ray; 2.76 A; D=1-1317.
DR   PDBsum; 5TW1; -.
DR   ProteinModelPortal; A0QS66; -.
DR   IntAct; A0QS66; 2.
DR   STRING; 246196.MSMEG_1368; -.
DR   PRIDE; A0QS66; -.
DR   EnsemblBacteria; ABK71951; ABK71951; MSMEG_1368.
DR   EnsemblBacteria; AFP37802; AFP37802; MSMEI_1329.
DR   GeneID; 4532832; -.
DR   KEGG; msb:LJ00_06820; -.
DR   KEGG; msg:MSMEI_1329; -.
DR   KEGG; msm:MSMEG_1368; -.
DR   PATRIC; 18075191; VBIMycSme59918_1352.
DR   eggNOG; ENOG4105D27; Bacteria.
DR   eggNOG; COG0086; LUCA.
DR   HOGENOM; HOG000218386; -.
DR   KO; K03046; -.
DR   OMA; YFAAYMI; -.
DR   OrthoDB; POG091H02K5; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   HAMAP; MF_01322; RNApol_bact_RpoC; 1.
DR   InterPro; IPR012754; DNA-dir_RpoC_beta_prime.
DR   InterPro; IPR000722; RNA_pol_asu.
DR   InterPro; IPR006592; RNA_pol_N.
DR   InterPro; IPR007080; RNA_pol_Rpb1_1.
DR   InterPro; IPR007066; RNA_pol_Rpb1_3.
DR   InterPro; IPR007083; RNA_pol_Rpb1_4.
DR   InterPro; IPR007081; RNA_pol_Rpb1_5.
DR   Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR   Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR   Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR   Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR   Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR   SMART; SM00663; RPOLA_N; 1.
DR   TIGRFAMs; TIGR02386; rpoC_TIGR; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; DNA-directed RNA polymerase;
KW   Nucleotidyltransferase; Reference proteome; Transcription;
KW   Transferase.
FT   CHAIN         1   1317       DNA-directed RNA polymerase subunit
FT                                beta'.
FT                                /FTId=PRO_0000308855.
FT   HELIX        17     23       {ECO:0000244|PDB:5TW1}.
FT   STRAND       24     27       {ECO:0000244|PDB:5TW1}.
FT   TURN         36     38       {ECO:0000244|PDB:5TW1}.
FT   TURN         49     52       {ECO:0000244|PDB:5TW1}.
FT   TURN         76     78       {ECO:0000244|PDB:5TW1}.
FT   HELIX        87     90       {ECO:0000244|PDB:5TW1}.
FT   STRAND       92    102       {ECO:0000244|PDB:5TW1}.
FT   HELIX       104    108       {ECO:0000244|PDB:5TW1}.
FT   STRAND      109    111       {ECO:0000244|PDB:5TW1}.
FT   HELIX       113    118       {ECO:0000244|PDB:5TW1}.
FT   HELIX       122    129       {ECO:0000244|PDB:5TW1}.
FT   STRAND      132    139       {ECO:0000244|PDB:5TW1}.
FT   HELIX       141    146       {ECO:0000244|PDB:5TW1}.
FT   HELIX       148    186       {ECO:0000244|PDB:5TW1}.
FT   HELIX       191    228       {ECO:0000244|PDB:5TW1}.
FT   HELIX       239    246       {ECO:0000244|PDB:5TW1}.
FT   HELIX       249    251       {ECO:0000244|PDB:5TW1}.
FT   STRAND      252    255       {ECO:0000244|PDB:5TW1}.
FT   HELIX       257    266       {ECO:0000244|PDB:5TW1}.
FT   HELIX       270    283       {ECO:0000244|PDB:5TW1}.
FT   HELIX       287    304       {ECO:0000244|PDB:5TW1}.
FT   HELIX       309    312       {ECO:0000244|PDB:5TW1}.
FT   STRAND      313    319       {ECO:0000244|PDB:5TW1}.
FT   HELIX       322    324       {ECO:0000244|PDB:5TW1}.
FT   STRAND      327    329       {ECO:0000244|PDB:5TW1}.
FT   STRAND      335    337       {ECO:0000244|PDB:5TW1}.
FT   HELIX       339    360       {ECO:0000244|PDB:5TW1}.
FT   HELIX       364    382       {ECO:0000244|PDB:5TW1}.
FT   STRAND      383    389       {ECO:0000244|PDB:5TW1}.
FT   HELIX       394    396       {ECO:0000244|PDB:5TW1}.
FT   HELIX       402    406       {ECO:0000244|PDB:5TW1}.
FT   HELIX       412    416       {ECO:0000244|PDB:5TW1}.
FT   STRAND      418    432       {ECO:0000244|PDB:5TW1}.
FT   STRAND      440    444       {ECO:0000244|PDB:5TW1}.
FT   HELIX       445    462       {ECO:0000244|PDB:5TW1}.
FT   HELIX       469    477       {ECO:0000244|PDB:5TW1}.
FT   HELIX       483    491       {ECO:0000244|PDB:5TW1}.
FT   STRAND      496    499       {ECO:0000244|PDB:5TW1}.
FT   HELIX       506    508       {ECO:0000244|PDB:5TW1}.
FT   STRAND      509    524       {ECO:0000244|PDB:5TW1}.
FT   HELIX       529    532       {ECO:0000244|PDB:5TW1}.
FT   TURN        536    538       {ECO:0000244|PDB:5TW1}.
FT   STRAND      540    544       {ECO:0000244|PDB:5TW1}.
FT   HELIX       549    557       {ECO:0000244|PDB:5TW1}.
FT   HELIX       561    563       {ECO:0000244|PDB:5TW1}.
FT   TURN        568    570       {ECO:0000244|PDB:5TW1}.
FT   STRAND      573    576       {ECO:0000244|PDB:5TW1}.
FT   HELIX       580    589       {ECO:0000244|PDB:5TW1}.
FT   STRAND      610    613       {ECO:0000244|PDB:5TW1}.
FT   HELIX       615    623       {ECO:0000244|PDB:5TW1}.
FT   TURN        624    626       {ECO:0000244|PDB:5TW1}.
FT   STRAND      634    641       {ECO:0000244|PDB:5TW1}.
FT   HELIX       645    650       {ECO:0000244|PDB:5TW1}.
FT   STRAND      652    654       {ECO:0000244|PDB:5TW1}.
FT   STRAND      661    664       {ECO:0000244|PDB:5TW1}.
FT   HELIX       667    673       {ECO:0000244|PDB:5TW1}.
FT   HELIX       689    702       {ECO:0000244|PDB:5TW1}.
FT   HELIX       705    725       {ECO:0000244|PDB:5TW1}.
FT   TURN        732    734       {ECO:0000244|PDB:5TW1}.
FT   HELIX       741    760       {ECO:0000244|PDB:5TW1}.
FT   HELIX       766    791       {ECO:0000244|PDB:5TW1}.
FT   HELIX       797    803       {ECO:0000244|PDB:5TW1}.
FT   HELIX       810    816       {ECO:0000244|PDB:5TW1}.
FT   STRAND      828    830       {ECO:0000244|PDB:5TW1}.
FT   TURN        839    841       {ECO:0000244|PDB:5TW1}.
FT   HELIX       845    880       {ECO:0000244|PDB:5TW1}.
FT   STRAND      885    888       {ECO:0000244|PDB:5TW1}.
FT   STRAND      896    898       {ECO:0000244|PDB:5TW1}.
FT   TURN        916    921       {ECO:0000244|PDB:5TW1}.
FT   STRAND      924    926       {ECO:0000244|PDB:5TW1}.
FT   HELIX       945    954       {ECO:0000244|PDB:5TW1}.
FT   STRAND      959    961       {ECO:0000244|PDB:5TW1}.
FT   TURN        964    966       {ECO:0000244|PDB:5TW1}.
FT   STRAND      970    974       {ECO:0000244|PDB:5TW1}.
FT   HELIX       975    978       {ECO:0000244|PDB:5TW1}.
FT   TURN        982    984       {ECO:0000244|PDB:5TW1}.
FT   STRAND      985    987       {ECO:0000244|PDB:5TW1}.
FT   HELIX       994   1003       {ECO:0000244|PDB:5TW1}.
FT   HELIX      1004   1008       {ECO:0000244|PDB:5TW1}.
FT   HELIX      1029   1036       {ECO:0000244|PDB:5TW1}.
FT   STRAND     1042   1045       {ECO:0000244|PDB:5TW1}.
FT   STRAND     1052   1058       {ECO:0000244|PDB:5TW1}.
FT   STRAND     1063   1069       {ECO:0000244|PDB:5TW1}.
FT   STRAND     1076   1078       {ECO:0000244|PDB:5TW1}.
FT   HELIX      1083   1085       {ECO:0000244|PDB:5TW1}.
FT   STRAND     1113   1117       {ECO:0000244|PDB:5TW1}.
FT   HELIX      1119   1126       {ECO:0000244|PDB:5TW1}.
FT   HELIX      1128   1145       {ECO:0000244|PDB:5TW1}.
FT   HELIX      1152   1162       {ECO:0000244|PDB:5TW1}.
FT   STRAND     1163   1169       {ECO:0000244|PDB:5TW1}.
FT   STRAND     1172   1175       {ECO:0000244|PDB:5TW1}.
FT   STRAND     1181   1183       {ECO:0000244|PDB:5TW1}.
FT   HELIX      1184   1192       {ECO:0000244|PDB:5TW1}.
FT   STRAND     1204   1207       {ECO:0000244|PDB:5TW1}.
FT   HELIX      1211   1216       {ECO:0000244|PDB:5TW1}.
FT   HELIX      1221   1227       {ECO:0000244|PDB:5TW1}.
FT   HELIX      1230   1240       {ECO:0000244|PDB:5TW1}.
FT   HELIX      1249   1255       {ECO:0000244|PDB:5TW1}.
FT   HELIX      1262   1264       {ECO:0000244|PDB:5TW1}.
FT   HELIX      1266   1269       {ECO:0000244|PDB:5TW1}.
FT   STRAND     1272   1275       {ECO:0000244|PDB:5TW1}.
FT   HELIX      1277   1282       {ECO:0000244|PDB:5TW1}.
SQ   SEQUENCE   1317 AA;  146513 MW;  2C9C7E156E76FBEA CRC64;
     MLDVNFFDEL RIGLATADDI RNWSYGEVKK PETINYRTLK PEKDGLFCEK IFGPTRDWEC
     YCGKYKRVRF KGIICERCGV EVTRAKVRRE RMGHIELAAP VTHIWYFKGV PSRLGYLLDL
     APKDLEKIIY FAAYVITSVD DEMRHNELST LEAEMAVEKK AVEDQRDADL EARAQKLEAD
     LAELEAEGAK SDVRRKVRDS GEREMRQLRD RAQRELDRLD EIWNTFTKLA PKQLIVDEVL
     YRELQDRYGE YFTGAMGAES IKKLIENFDI DAEAESLREV IRSGKGQKKL RALKRLKVVA
     AFQQSGNSPM GMVLDAVPVI PPELRPMVQL DGGRFATSDL NDLYRRVINR NNRLKRLIDL
     GAPEIIVNNE KRMLQESVDA LFDNGRRGRP VTGPGNRPLK SLSDLLKGKQ GRFRQNLLGK
     RVDYSGRSVI VVGPQLKLHQ CGLPKLMALE LFKPFVMKRL VDLNHAQNIK SAKRMVERQR
     PQVWDVLEEV IAEHPVLLNR APTLHRLGIQ AFEPQLVEGK AIQLHPLVCE AFNADFDGDQ
     MAVHLPLSAE AQAEARILML SSNNILSPAS GKPLAMPRLD MVTGLYYLTT LVEGATGEYQ
     AATKDAPEQG VYSSPAEAIM AMDRGALSVR AKIKVRLTEL RPPTDLEAQL FENGWKPGDA
     WTAETTLGRV MFNELLPKSY PFVNEQMHKK VQARIINDLA ERFPMIVVAQ TVDKLKDAGF
     YWATRSGVTV SMADVLVPPQ KQEILERHEA EADAIERKYQ RGALNHTERN ESLVKIWQDA
     TEEVGKALEE FYPADNPIIT IVKSGATGNL TQTRTLAGMK GLVTNPKGEF IPRPIKSSFR
     EGLTVLEYFI NTHGARKGLA DTALRTADSG YLTRRLVDVS QDVIVREHDC ETERGINVTL
     AERGPDGTLI RDAHVETSAF ARTLATDAVD ANGNVIIERG HDLGDPAIDA LLAAGITTVK
     VRSVLTCTSA TGVCAMCYGR SMATGKLVDI GEAVGIVAAQ SIGEPGTQLT MRTFHQGGVT
     GGADIVGGLP RVQELFEARV PRNKAPIADV AGRVRLEESD KFFKITIVPD DGGEEVVYDK
     LSKRQRLRVI THEDGTEGVL SDGDHVEVGD QLMEGAADPH EVLRVQGPRE VQIHLVKEVQ
     EVYRAQGVSI HDKHIEVIVR QMLRRVTIID SGSTEFLPGS LTERAEFEAE NRRVVAEGGE
     PAAGRPVLMG ITKASLATDS WLSAASFQET TRVLTDAAIN CRSDKLNGLK ENVIIGKLIP
     AGTGISRYRN IQVQPTEEAR AAAYTIPSYE DQYYSPDFGQ ATGAAVPLDD YGYSDYR
//
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