ID RPOC_MYCS2 Reviewed; 1317 AA.
AC A0QS66; I7FY06;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 01-MAY-2013, entry version 50.
DE RecName: Full=DNA-directed RNA polymerase subunit beta';
DE Short=RNAP subunit beta';
DE EC=2.7.7.6;
DE AltName: Full=RNA polymerase subunit beta';
DE AltName: Full=Transcriptase subunit beta';
GN Name=rpoC; OrderedLocusNames=MSMEG_1368, MSMEI_1329;
OS Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155).
OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC Corynebacterineae; Mycobacteriaceae; Mycobacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS], AND IDENTIFICATION OF N-TERMINUS.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through
RT orthology and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription
CC of DNA into RNA using the four ribonucleoside triphosphates as
CC substrates (By similarity).
CC -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC + RNA(n+1).
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1
CC beta' and 1 omega subunit. When a sigma factor is associated with
CC the core the holoenzyme is formed, which can initiate
CC transcription (By similarity).
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
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DR EMBL; CP000480; ABK71951.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP37802.1; -; Genomic_DNA.
DR RefSeq; YP_006566097.1; NC_018289.1.
DR RefSeq; YP_885754.1; NC_008596.1.
DR ProteinModelPortal; A0QS66; -.
DR SMR; A0QS66; 9-136.
DR IntAct; A0QS66; 2.
DR STRING; 246196.MSMEG_1368; -.
DR PRIDE; A0QS66; -.
DR EnsemblBacteria; ABK71951; ABK71951; MSMEG_1368.
DR GeneID; 13429144; -.
DR GeneID; 4532832; -.
DR KEGG; msg:MSMEI_1329; -.
DR KEGG; msm:MSMEG_1368; -.
DR PATRIC; 18075191; VBIMycSme59918_1352.
DR eggNOG; COG0086; -.
DR HOGENOM; HOG000218386; -.
DR KO; K03046; -.
DR OMA; DINDKHI; -.
DR ProtClustDB; PRK00566; -.
DR BioCyc; MSME246196:GJ4Y-1368-MONOMER; -.
DR GO; GO:0003677; F:DNA binding; IEA:HAMAP.
DR GO; GO:0003899; F:DNA-directed RNA polymerase activity; IEA:HAMAP.
DR GO; GO:0006351; P:transcription, DNA-dependent; IEA:HAMAP.
DR Gene3D; 2.40.40.20; -; 2.
DR HAMAP; MF_01322; RNApol_bact_RpoC; 1; -.
DR InterPro; IPR009010; Asp_de-COase-like_dom.
DR InterPro; IPR012754; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR TIGRFAMs; TIGR02386; rpoC_TIGR; 1.
PE 1: Evidence at protein level;
KW Complete proteome; DNA-directed RNA polymerase;
KW Nucleotidyltransferase; Reference proteome; Transcription;
KW Transferase.
FT CHAIN 1 1317 DNA-directed RNA polymerase subunit
FT beta'.
FT /FTId=PRO_0000308855.
SQ SEQUENCE 1317 AA; 146513 MW; 2C9C7E156E76FBEA CRC64;
MLDVNFFDEL RIGLATADDI RNWSYGEVKK PETINYRTLK PEKDGLFCEK IFGPTRDWEC
YCGKYKRVRF KGIICERCGV EVTRAKVRRE RMGHIELAAP VTHIWYFKGV PSRLGYLLDL
APKDLEKIIY FAAYVITSVD DEMRHNELST LEAEMAVEKK AVEDQRDADL EARAQKLEAD
LAELEAEGAK SDVRRKVRDS GEREMRQLRD RAQRELDRLD EIWNTFTKLA PKQLIVDEVL
YRELQDRYGE YFTGAMGAES IKKLIENFDI DAEAESLREV IRSGKGQKKL RALKRLKVVA
AFQQSGNSPM GMVLDAVPVI PPELRPMVQL DGGRFATSDL NDLYRRVINR NNRLKRLIDL
GAPEIIVNNE KRMLQESVDA LFDNGRRGRP VTGPGNRPLK SLSDLLKGKQ GRFRQNLLGK
RVDYSGRSVI VVGPQLKLHQ CGLPKLMALE LFKPFVMKRL VDLNHAQNIK SAKRMVERQR
PQVWDVLEEV IAEHPVLLNR APTLHRLGIQ AFEPQLVEGK AIQLHPLVCE AFNADFDGDQ
MAVHLPLSAE AQAEARILML SSNNILSPAS GKPLAMPRLD MVTGLYYLTT LVEGATGEYQ
AATKDAPEQG VYSSPAEAIM AMDRGALSVR AKIKVRLTEL RPPTDLEAQL FENGWKPGDA
WTAETTLGRV MFNELLPKSY PFVNEQMHKK VQARIINDLA ERFPMIVVAQ TVDKLKDAGF
YWATRSGVTV SMADVLVPPQ KQEILERHEA EADAIERKYQ RGALNHTERN ESLVKIWQDA
TEEVGKALEE FYPADNPIIT IVKSGATGNL TQTRTLAGMK GLVTNPKGEF IPRPIKSSFR
EGLTVLEYFI NTHGARKGLA DTALRTADSG YLTRRLVDVS QDVIVREHDC ETERGINVTL
AERGPDGTLI RDAHVETSAF ARTLATDAVD ANGNVIIERG HDLGDPAIDA LLAAGITTVK
VRSVLTCTSA TGVCAMCYGR SMATGKLVDI GEAVGIVAAQ SIGEPGTQLT MRTFHQGGVT
GGADIVGGLP RVQELFEARV PRNKAPIADV AGRVRLEESD KFFKITIVPD DGGEEVVYDK
LSKRQRLRVI THEDGTEGVL SDGDHVEVGD QLMEGAADPH EVLRVQGPRE VQIHLVKEVQ
EVYRAQGVSI HDKHIEVIVR QMLRRVTIID SGSTEFLPGS LTERAEFEAE NRRVVAEGGE
PAAGRPVLMG ITKASLATDS WLSAASFQET TRVLTDAAIN CRSDKLNGLK ENVIIGKLIP
AGTGISRYRN IQVQPTEEAR AAAYTIPSYE DQYYSPDFGQ ATGAAVPLDD YGYSDYR
//
