ID RSMA_ESCF3 Reviewed; 273 AA.
AC B7LVU5;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 1.
DT 01-MAY-2013, entry version 34.
DE RecName: Full=Ribosomal RNA small subunit methyltransferase A;
DE EC=2.1.1.182;
DE AltName: Full=16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase;
DE AltName: Full=16S rRNA dimethyladenosine transferase;
DE AltName: Full=16S rRNA dimethylase;
DE AltName: Full=S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase;
GN Name=rsmA; Synonyms=ksgA; OrderedLocusNames=EFER_0062;
OS Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=585054;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35469 / DSM 13698 / CDC 0568-73;
RX PubMed=19165319; DOI=10.1371/journal.pgen.1000344;
RA Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P.,
RA Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A.,
RA Chiapello H., Clermont O., Cruveiller S., Danchin A., Diard M.,
RA Dossat C., Karoui M.E., Frapy E., Garry L., Ghigo J.M., Gilles A.M.,
RA Johnson J., Le Bouguenec C., Lescat M., Mangenot S.,
RA Martinez-Jehanne V., Matic I., Nassif X., Oztas S., Petit M.A.,
RA Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J., Vacherie B.,
RA Vallenet D., Medigue C., Rocha E.P.C., Denamur E.;
RT "Organised genome dynamics in the Escherichia coli species results in
RT highly diverse adaptive paths.";
RL PLoS Genet. 5:E1000344-E1000344(2009).
CC -!- FUNCTION: Specifically dimethylates two adjacent adenosines (A1518
CC and A1519) in the loop of a conserved hairpin near the 3'-end of
CC 16S rRNA in the 30S particle. May play a critical role in
CC biogenesis of 30S subunits (By similarity).
CC -!- CATALYTIC ACTIVITY: 4 S-adenosyl-L-methionine +
CC adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-
CC homocysteine + N(6)-dimethyladenine(1518)/N(6)-
CC dimethyladenine(1519) in 16S rRNA.
CC -!- SUBCELLULAR LOCATION: Cytoplasm (Potential).
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. rRNA
CC adenine N(6)-methyltransferase family. RsmA subfamily.
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DR EMBL; CU928158; CAQ87648.1; -; Genomic_DNA.
DR RefSeq; YP_002381292.1; NC_011740.1.
DR STRING; 585054.EFER_0062; -.
DR EnsemblBacteria; CAQ87648; CAQ87648; EFER_0062.
DR GeneID; 7120521; -.
DR KEGG; efe:EFER_0062; -.
DR PATRIC; 32124551; VBIEscFer122920_0064.
DR eggNOG; COG0030; -.
DR HOGENOM; HOG000227962; -.
DR KO; K02528; -.
DR ProtClustDB; PRK00274; -.
DR BioCyc; EFER585054:GJJM-63-MONOMER; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0052908; F:16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity; IEA:EC.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000179; F:rRNA (adenine-N6,N6-)-dimethyltransferase activity; IEA:InterPro.
DR GO; GO:0031167; P:rRNA methylation; IEA:GOC.
DR Gene3D; 1.10.8.100; -; 1.
DR HAMAP; MF_00607; 16SrRNA_methyltr_A; 1; -.
DR InterPro; IPR023165; rRNA_Ade_diMease-like.
DR InterPro; IPR020596; rRNA_Ade_Mease_Trfase_CS.
DR InterPro; IPR001737; rRNA_Ade_methylase_transferase.
DR InterPro; IPR020598; rRNA_Ade_methylase_Trfase_N.
DR InterPro; IPR011530; rRNA_adenine_dimethylase.
DR PANTHER; PTHR11727; PTHR11727; 1.
DR Pfam; PF00398; RrnaAD; 1.
DR SMART; SM00650; rADc; 1.
DR TIGRFAMs; TIGR00755; ksgA; 1.
DR PROSITE; PS01131; RRNA_A_DIMETH; 1.
PE 3: Inferred from homology;
KW Complete proteome; Cytoplasm; Methyltransferase; RNA-binding;
KW rRNA processing; S-adenosyl-L-methionine; Transferase.
FT CHAIN 1 273 Ribosomal RNA small subunit
FT methyltransferase A.
FT /FTId=PRO_1000130278.
FT BINDING 18 18 S-adenosyl-L-methionine; via carbonyl
FT oxygen (By similarity).
FT BINDING 20 20 S-adenosyl-L-methionine; via amide
FT nitrogen (By similarity).
FT BINDING 45 45 S-adenosyl-L-methionine; via carbonyl
FT oxygen (By similarity).
FT BINDING 66 66 S-adenosyl-L-methionine (By similarity).
FT BINDING 91 91 S-adenosyl-L-methionine (By similarity).
FT BINDING 113 113 S-adenosyl-L-methionine (By similarity).
SQ SEQUENCE 273 AA; 30446 MW; 5BA476A0E1011C87 CRC64;
MNNRVHQGHL ARKRFGQNFL NDQFVIDSIV SAINPQKGQA MVEIGPGLAA LTEPVGERLD
QLTVIELDRD LAARLQTHPF LGPKLTIYQQ DAMTFNFGEL AEKMGQPLRV FGNLPYNIST
PLMFHLFSYT DAIADMHFML QKEVVNRLVA GPNSKAYGRL SVMAQYYCNV IPVLEVPPSA
FTPPPKVDSA VVRLVPHATM PYPVKDVRVL SRITTEAFNQ RRKTIRNSLG NLFSVEVLTG
MGIDPAMRAE NISVAQYCQM ANYLAENAPL QES
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