ID RSMB_YERPA Reviewed; 429 AA.
AC Q1C2X7;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 1.
DT 01-MAY-2013, entry version 55.
DE RecName: Full=Ribosomal RNA small subunit methyltransferase B;
DE EC=2.1.1.176;
DE AltName: Full=16S rRNA m5C967 methyltransferase;
DE AltName: Full=rRNA (cytosine-C(5)-)-methyltransferase RsmB;
GN Name=rsmB; Synonyms=sun; OrderedLocusNames=YPA_3233;
OS Yersinia pestis bv. Antiqua (strain Antiqua).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Yersinia.
OX NCBI_TaxID=360102;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Antiqua;
RX PubMed=16740952; DOI=10.1128/JB.00124-06;
RA Chain P.S.G., Hu P., Malfatti S.A., Radnedge L., Larimer F.,
RA Vergez L.M., Worsham P., Chu M.C., Andersen G.L.;
RT "Complete genome sequence of Yersinia pestis strains Antiqua and
RT Nepal516: evidence of gene reduction in an emerging pathogen.";
RL J. Bacteriol. 188:4453-4463(2006).
CC -!- FUNCTION: Specifically methylates the cytosine at position 967
CC (m5C967) of 16S rRNA (By similarity).
CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + cytosine(967) in 16S
CC rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(967) in 16S
CC rRNA.
CC -!- SUBCELLULAR LOCATION: Cytoplasm (Potential).
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. RsmB/NOP
CC family.
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DR EMBL; CP000308; ABG15195.1; -; Genomic_DNA.
DR RefSeq; YP_653140.1; NC_008150.1.
DR ProteinModelPortal; Q1C2X7; -.
DR SMR; Q1C2X7; 6-428.
DR STRING; 360102.YPA_3233; -.
DR PRIDE; Q1C2X7; -.
DR EnsemblBacteria; ABG15195; ABG15195; YPA_3233.
DR GeneID; 4118502; -.
DR KEGG; ypa:YPA_3233; -.
DR PATRIC; 18586318; VBIYerPes1796_3954.
DR eggNOG; COG0144; -.
DR HOGENOM; HOG000037300; -.
DR KO; K03500; -.
DR OMA; KPPMWLR; -.
DR ProtClustDB; PRK10901; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016434; F:rRNA (cytosine) methyltransferase activity; IEA:HAMAP.
DR GO; GO:0006355; P:regulation of transcription, DNA-dependent; IEA:InterPro.
DR Gene3D; 1.10.940.10; -; 1.
DR HAMAP; MF_01856; 16SrRNA_methyltr_B; 1; -.
DR InterPro; IPR001678; Fmu/NOL1/Nop2p.
DR InterPro; IPR018314; Fmu/NOL1/Nop2p_CS.
DR InterPro; IPR006027; NusB_RsmB_TIM44.
DR InterPro; IPR023267; RCMT.
DR InterPro; IPR004573; rRNA_ssu_MeTfrase_B.
DR InterPro; IPR023541; rRNA_ssu_MeTfrase_B_ent.
DR Pfam; PF01189; Nol1_Nop2_Fmu; 1.
DR Pfam; PF01029; NusB; 1.
DR PRINTS; PR02008; RCMTFAMILY.
DR SUPFAM; SSF48013; NusB_RsmB_TIM44; 1.
DR TIGRFAMs; TIGR00563; rsmB; 1.
DR PROSITE; PS01153; NOL1_NOP2_SUN; 1.
PE 3: Inferred from homology;
KW Complete proteome; Cytoplasm; Methyltransferase; RNA-binding;
KW rRNA processing; S-adenosyl-L-methionine; Transferase.
FT CHAIN 1 429 Ribosomal RNA small subunit
FT methyltransferase B.
FT /FTId=PRO_0000366185.
FT REGION 254 260 S-adenosyl-L-methionine binding (By
FT similarity).
FT ACT_SITE 375 375 Nucleophile (Potential).
FT BINDING 277 277 S-adenosyl-L-methionine (By similarity).
FT BINDING 303 303 S-adenosyl-L-methionine (By similarity).
FT BINDING 322 322 S-adenosyl-L-methionine (By similarity).
SQ SEQUENCE 429 AA; 48444 MW; 0E18231A62D8782E CRC64;
MKNTYNLRSI AAKAISQVLD QGQSLSAVLP ELQKNISDKD RALLQELCFG TLRVLPQLEW
CIQQLMARPM TGKQRVFHYL IMVGLYQLIY TRIPPHAALA ETVEGATVLK RPQLKGLING
VLRQFQRQQV ELLERAVNND SHYLHPSWLL ARIKQAYPAQ WQQILDANNQ RPPMWLRVNR
LHHSRSEYLE LLTQADINAE PHPIYRDAVR LITPCAVNHL PGFELGWVTV QDASAQGCVD
LLDPQNGEQI LDLCAAPGGK TTHILEAAPK AHVLAVDIDE QRLSRVKENL QRLQLQAVVR
VGDGRAPDTW CGDQQFDRIL LDAPCSATGV IRRHPDIKWL RRDRDISELA QLQSEIIEAI
WPKLKHGGVL VYATCSILPE ENQQQIAAFL QRHPEAQLTE TGTTAAPGKQ NLPHPEDGDG
FFYAKIIKK
//
