ID S22A2_PIG Reviewed; 554 AA.
AC O02713;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-APR-2013, entry version 83.
DE RecName: Full=Solute carrier family 22 member 2;
DE AltName: Full=Apical organic cation transporter;
DE AltName: Full=Organic cation transporter 2;
GN Name=SLC22A2; Synonyms=OCT2;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Suina; Suidae;
OC Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Kidney;
RX PubMed=9099681; DOI=10.1074/jbc.272.16.10408;
RA Grundemann D., Babin-Ebell J., Martel F., Ording N., Schmidt A.,
RA Schoemig E.;
RT "Primary structure and functional expression of the apical organic
RT cation transporter from kidney epithelial LLC-PK1 cells.";
RL J. Biol. Chem. 272:10408-10413(1997).
CC -!- FUNCTION: Mediates tubular uptake of organic compounds from
CC circulation. Mediates the influx of agmatine, dopamine,
CC noradrenaline (norepinephrine), serotonin, choline, famotidine,
CC ranitidine, histamin, creatinine, amantadine, memantine,
CC acriflavine, 4-[4-(dimethylamino)-styryl]-N-methylpyridinium ASP,
CC amiloride, metformin, N-1-methylnicotinamide (NMN),
CC tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP),
CC cimetidine, cisplatin and oxaliplatin. Cisplatin may develop a
CC nephrotoxic action. Transport of creatinine is inhibited by
CC fluoroquinolones such as DX-619 and LVFX. This transporter is a
CC major determinant of the anticancer activity of oxaliplatin and
CC may contribute to antitumor specificity (By similarity).
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein
CC (Potential).
CC -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1)
CC superfamily. Organic cation transporter (TC 2.A.1.19) family.
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DR EMBL; Y09400; CAA70567.1; -; mRNA.
DR RefSeq; NP_999067.1; NM_213902.1.
DR UniGene; Ssc.14537; -.
DR ProteinModelPortal; O02713; -.
DR STRING; 9823.ENSSSCP00000004366; -.
DR TCDB; 2.A.1.19.5; major facilitator superfamily (MFS).
DR GeneID; 396936; -.
DR KEGG; ssc:396936; -.
DR CTD; 6582; -.
DR eggNOG; COG0477; -.
DR HOGENOM; HOG000234568; -.
DR HOVERGEN; HBG061545; -.
DR KO; K08199; -.
DR OrthoDB; EOG4S4PG6; -.
DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR GO; GO:0015075; F:ion transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR016196; MFS_dom_general_subst_transpt.
DR InterPro; IPR004749; Orgcat_transp.
DR InterPro; IPR005828; Sub_transporter.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; MFS_gen_substrate_transporter; 1.
DR TIGRFAMs; TIGR00898; 2A0119; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 2.
DR PROSITE; PS00217; SUGAR_TRANSPORT_2; FALSE_NEG.
PE 2: Evidence at transcript level;
KW Complete proteome; Glycoprotein; Ion transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1 554 Solute carrier family 22 member 2.
FT /FTId=PRO_0000320959.
FT TOPO_DOM 1 21 Cytoplasmic (Potential).
FT TRANSMEM 22 42 Helical; (Potential).
FT TOPO_DOM 43 149 Extracellular (Potential).
FT TRANSMEM 150 170 Helical; (Potential).
FT TOPO_DOM 171 176 Cytoplasmic (Potential).
FT TRANSMEM 177 197 Helical; (Potential).
FT TOPO_DOM 198 209 Extracellular (Potential).
FT TRANSMEM 210 230 Helical; (Potential).
FT TOPO_DOM 231 237 Cytoplasmic (Potential).
FT TRANSMEM 238 258 Helical; (Potential).
FT TOPO_DOM 259 262 Extracellular (Potential).
FT TRANSMEM 263 283 Helical; (Potential).
FT TOPO_DOM 284 347 Cytoplasmic (Potential).
FT TRANSMEM 348 368 Helical; (Potential).
FT TOPO_DOM 369 376 Extracellular (Potential).
FT TRANSMEM 377 397 Helical; (Potential).
FT TOPO_DOM 398 403 Cytoplasmic (Potential).
FT TRANSMEM 404 424 Helical; (Potential).
FT TOPO_DOM 425 431 Extracellular (Potential).
FT TRANSMEM 432 452 Helical; (Potential).
FT TOPO_DOM 453 463 Cytoplasmic (Potential).
FT TRANSMEM 464 484 Helical; (Potential).
FT TOPO_DOM 485 493 Extracellular (Potential).
FT TRANSMEM 494 514 Helical; (Potential).
FT TOPO_DOM 515 554 Cytoplasmic (Potential).
FT SITE 450 450 Involved in recognition of organic
FT cations and participates in structural
FT changes that occur during translocation
FT of organic cations (By similarity).
FT CARBOHYD 71 71 N-linked (GlcNAc...) (Potential).
SQ SEQUENCE 554 AA; 61990 MW; E14B5565600C553B CRC64;
MLTVDDILEH TGEFNFFQKQ TFFLLALLSA AFTPIYVGIV FLGFIPDHRC RSPGVAELSQ
RCGWSLAEEL NYTVPGPGPA GQAFPRQCRR YEVDWNQSTL GCVDPLAGLA ANSSHLPLGP
CRYGWVYDTP GSSIVTEFDL VCANSWLLDL FQSAVNVGFF IGSVGIGYIA DRFGRKLCLL
LTILINAVSG VLMAISPTYT WMLVFRLIQG LVSKAGWMIG YILITEFVGL SYRRTVGIFY
QVAFTFGLLV LAGVAYALPH WRWLQFTVTL PNFCFLFYYW CVPESPRWLI SQNKNAKAMS
IIKHIAKKNG KSLPASLQSL RPDEEVGEKL KPSFLDLVRT PQIRKHTLIL MYNWFTSAVL
YQGLVMHMGL AGSNLYLDFF YSALVEFPAA LLILLTIDRL GRRHPWAASN VVAGAACLAS
VFIPEDPHWL RITVLCLGRM GITMAYEMVC LVNAELYPTF IRNLGVLVCS SMCDIGGIIT
PFLVYRLTDI WHELPLVVFA VVGLIAGGLV LLLPETKGKT LPETIEEAET MRRPRKNKEK
IIYLQVKKLD IPPN
//
