ID TRMA_KLEP7 Reviewed; 366 AA.
AC A6TGE9;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 21-AUG-2007, sequence version 1.
DT 29-MAY-2013, entry version 40.
DE RecName: Full=tRNA (uracil(54)-C(5))-methyltransferase;
DE EC=2.1.1.35;
DE AltName: Full=tRNA(m5U54)-methyltransferase;
DE Short=RUMT;
GN Name=trmA; OrderedLocusNames=KPN78578_42090; ORFNames=KPN_04254;
OS Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH
OS 78578).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Klebsiella.
OX NCBI_TaxID=272620;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700721 / MGH 78578;
RG The Klebsiella pneumonia Genome Sequencing Project;
RA McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P.,
RA Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K.;
RL Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the formation of 5-methyl-uridine at position
CC 54 (m5U54) in all tRNAs (By similarity).
CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + uridine(54) in tRNA
CC = S-adenosyl-L-homocysteine + 5-methyluridine(54) in tRNA.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. RNA M5U
CC methyltransferase family. TrmA subfamily.
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DR EMBL; CP000647; ABR79633.1; -; Genomic_DNA.
DR RefSeq; YP_001337900.1; NC_009648.1.
DR ProteinModelPortal; A6TGE9; -.
DR SMR; A6TGE9; 1-366.
DR STRING; 272620.KPN_04254; -.
DR EnsemblBacteria; ABR79633; ABR79633; KPN_04254.
DR GeneID; 5342032; -.
DR KEGG; kpn:KPN_04254; -.
DR PATRIC; 20462968; VBIKlePne13394_4322.
DR eggNOG; COG2265; -.
DR HOGENOM; HOG000218626; -.
DR KO; K00557; -.
DR OMA; ESGVWLI; -.
DR ProtClustDB; PRK05031; -.
DR BioCyc; KPNE272620:GKDC-4291-MONOMER; -.
DR GO; GO:0030697; F:S-adenosylmethionine-dependent tRNA (m5U54) methyltransferase activity; IEA:HAMAP.
DR HAMAP; MF_01011; tRNA_methyltr_TrmA; 1; -.
DR InterPro; IPR011869; TrmA_MeTrfase_proteobac.
DR InterPro; IPR010280; U5_MeTrfase.
DR Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR TIGRFAMs; TIGR02143; trmA_only; 1.
DR PROSITE; PS01230; TRMA_1; 1.
DR PROSITE; PS01231; TRMA_2; 1.
PE 3: Inferred from homology;
KW Complete proteome; Methyltransferase; S-adenosyl-L-methionine;
KW Transferase; tRNA processing.
FT CHAIN 1 366 tRNA (uracil(54)-C(5))-methyltransferase.
FT /FTId=PRO_1000072910.
FT ACT_SITE 324 324 Nucleophile (By similarity).
FT BINDING 190 190 S-adenosyl-L-methionine (By similarity).
FT BINDING 218 218 S-adenosyl-L-methionine; via carbonyl
FT oxygen (By similarity).
FT BINDING 223 223 S-adenosyl-L-methionine (By similarity).
FT BINDING 239 239 S-adenosyl-L-methionine (By similarity).
FT BINDING 299 299 S-adenosyl-L-methionine (By similarity).
SQ SEQUENCE 366 AA; 41845 MW; 76BE7481F1F70899 CRC64;
MTPEHLPTEQ YEAQLAEKVV RLQTMMAPFA APVPEVFRSP VSHYRMRAEF RLWHDGDDLY
HIIFDQQTRS RIRVDSFPAA SALINQLMTA MLEGVRNNPV LRQKLFQIDY LTTLSNQAVV
SVLYHKKLDD AWREQAEALR DALRAQGLNV HLIGRATKTK IELDQDYIDE RLPVGGREMI
YRQVENSFTQ PNAAMNIQML EWALDVTKGA TGDLLELYCG NGNFSLALAR NFDRVLATEI
AKPSVAAAQY NIAANHIDNV QIIRMAAEEF TQAMNGVRQF NRLQGIDLHS YQCETIFVDP
PRSGLDSETE KMVQAYPRIL YISCNPETLC RNLETLSQTH NVTRLALFDQ FPYTHHMECG
VLLTRK
//
