ID TRUA_METMJ Reviewed; 270 AA.
AC A3CXX4;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 01-MAY-2013, entry version 43.
DE RecName: Full=tRNA pseudouridine synthase A;
DE EC=5.4.99.12;
DE AltName: Full=tRNA pseudouridine(38-40) synthase;
DE AltName: Full=tRNA pseudouridylate synthase I;
DE AltName: Full=tRNA-uridine isomerase I;
GN Name=truA; OrderedLocusNames=Memar_2301;
OS Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1).
OC Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
OC Methanomicrobiaceae; Methanoculleus.
OX NCBI_TaxID=368407;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35101 / DSM 1498 / JR1;
RX PubMed=21304656; DOI=10.4056/sigs.32535;
RA Anderson I.J., Sieprawska-Lupa M., Lapidus A., Nolan M., Copeland A.,
RA Glavina Del Rio T., Tice H., Dalin E., Barry K., Saunders E., Han C.,
RA Brettin T., Detter J.C., Bruce D., Mikhailova N., Pitluck S.,
RA Hauser L., Land M., Lucas S., Richardson P., Whitman W.B.,
RA Kyrpides N.C.;
RT "Complete genome sequence of Methanoculleus marisnigri Romesser et al.
RT 1981 type strain JR1.";
RL Stand. Genomic Sci. 1:189-196(2009).
CC -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in
CC the anticodon stem and loop of transfer RNAs (By similarity).
CC -!- CATALYTIC ACTIVITY: tRNA uridine(38-40) = tRNA pseudouridine(38-
CC 40).
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA
CC family.
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DR EMBL; CP000562; ABN58224.1; -; Genomic_DNA.
DR RefSeq; YP_001048206.1; NC_009051.1.
DR ProteinModelPortal; A3CXX4; -.
DR STRING; 368407.Memar_2301; -.
DR EnsemblBacteria; ABN58224; ABN58224; Memar_2301.
DR GeneID; 4848144; -.
DR KEGG; mem:Memar_2301; -.
DR eggNOG; COG0101; -.
DR HOGENOM; HOG000248673; -.
DR KO; K06173; -.
DR OMA; DWLTPRA; -.
DR ProtClustDB; PRK14589; -.
DR BioCyc; MMAR368407:GH7L-2354-MONOMER; -.
DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:HAMAP.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:HAMAP.
DR Gene3D; 3.30.70.580; -; 1.
DR Gene3D; 3.30.70.660; -; 1.
DR HAMAP; MF_00171; TruA; 1; -.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR020094; PsdUridine_synth_TruA_N.
DR PANTHER; PTHR11142; PTHR11142; 1.
DR Pfam; PF01416; PseudoU_synth_1; 2.
DR PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR SUPFAM; SSF55120; PsdUridine_synth_cat_dom; 1.
DR TIGRFAMs; TIGR00071; hisT_truA; 1.
PE 3: Inferred from homology;
KW Complete proteome; Isomerase; tRNA processing.
FT CHAIN 1 270 tRNA pseudouridine synthase A.
FT /FTId=PRO_1000017115.
FT ACT_SITE 55 55 Nucleophile (By similarity).
FT BINDING 110 110 Substrate (By similarity).
SQ SEQUENCE 270 AA; 30287 MW; C33FD09EE5A10A2C CRC64;
MNLAFRFSYF GDRFFGSQMQ PDLCTVEGEF IGACRLLRLF DDWREANFAT AGRTDRGVHA
RSQVCSFLTD KPERAIEALN RVLPADIWCT GWAEAPDGFH PRYSAVSRTY RYYFSAPGDA
AAMHEAAQEF LGRHDFSAFA RAGDRNPERR ILASRVFIDG EFAVFEVTGE SFLWNMVRCM
ATMLGRVGRG EAEAGEIARL LTGPVERRVA AAPPEGLILW DIDYGIPFTP LPIDAGSSRH
LGDRHRYHVL MAKISAHLAQ DHRQPDTPGI
//
