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Database: UniProt/SWISS-PROT
Entry: TRUA_METRJ
LinkDB: TRUA_METRJ
Original site: TRUA_METRJ 
ID   TRUA_METRJ              Reviewed;         253 AA.
AC   B1M6N9;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   01-OCT-2014, entry version 45.
DE   RecName: Full=tRNA pseudouridine synthase A {ECO:0000255|HAMAP-Rule:MF_00171};
DE            EC=5.4.99.12 {ECO:0000255|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA pseudouridine(38-40) synthase {ECO:0000255|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA pseudouridylate synthase I {ECO:0000255|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA-uridine isomerase I {ECO:0000255|HAMAP-Rule:MF_00171};
GN   Name=truA {ECO:0000255|HAMAP-Rule:MF_00171};
GN   OrderedLocusNames=Mrad2831_3148;
OS   Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM
OS   2831).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Methylobacteriaceae; Methylobacterium.
OX   NCBI_TaxID=426355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27329 / DSM 1819 / JCM 2831;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E.,
RA   Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T.,
RA   Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Mikhailova N., Marx C.J., Richardson P.;
RT   "Complete sequence of chromosome of Methylobacterium radiotolerans JCM
RT   2831.";
RL   Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in
CC       the anticodon stem and loop of transfer RNAs. {ECO:0000255|HAMAP-
CC       Rule:MF_00171}.
CC   -!- CATALYTIC ACTIVITY: tRNA uridine(38-40) = tRNA pseudouridine(38-
CC       40). {ECO:0000255|HAMAP-Rule:MF_00171}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00171}.
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA
CC       family. {ECO:0000255|HAMAP-Rule:MF_00171}.
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DR   EMBL; CP001001; ACB25130.1; -; Genomic_DNA.
DR   RefSeq; WP_012320095.1; NC_010505.1.
DR   RefSeq; YP_001755813.1; NC_010505.1.
DR   ProteinModelPortal; B1M6N9; -.
DR   STRING; 426355.Mrad2831_3148; -.
DR   EnsemblBacteria; ACB25130; ACB25130; Mrad2831_3148.
DR   GeneID; 6139195; -.
DR   KEGG; mrd:Mrad2831_3148; -.
DR   PATRIC; 22574674; VBIMetRad70578_3218.
DR   eggNOG; COG0101; -.
DR   HOGENOM; HOG000248672; -.
DR   KO; K06173; -.
DR   OMA; WDPFRLS; -.
DR   OrthoDB; EOG6Z9B4R; -.
DR   BioCyc; MRAD426355:GJB5-3185-MONOMER; -.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-HAMAP.
DR   Gene3D; 3.30.70.580; -; 1.
DR   Gene3D; 3.30.70.660; -; 1.
DR   HAMAP; MF_00171; TruA; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom.
DR   InterPro; IPR001406; PsdUridine_synth_TruA.
DR   InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR   InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR   InterPro; IPR020094; PsdUridine_synth_TruA_N.
DR   PANTHER; PTHR11142; PTHR11142; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 2.
DR   PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00071; hisT_truA; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Isomerase; Reference proteome; tRNA processing.
FT   CHAIN         1    253       tRNA pseudouridine synthase A.
FT                                /FTId=PRO_1000097762.
FT   ACT_SITE     52     52       Nucleophile. {ECO:0000255|HAMAP-
FT                                Rule:MF_00171}.
FT   BINDING     111    111       Substrate. {ECO:0000255|HAMAP-
FT                                Rule:MF_00171}.
SQ   SEQUENCE   253 AA;  27832 MW;  EBF81A6552FC176C CRC64;
     MPRYKLVIEY DGSPFCGWQR QADDPTVQGA IEAAVTRFSG ENARLTCAGR TDAGVHAIHQ
     VAHLDLAKDW RTDTVRDALN AHLRPQPVAI LSAETVPDSF DARHSAIRRH YRYRILNRRS
     PAALTRAHVW HVPWPLDADL MHDAAQRLVG RHDFSAFRAA ECQANSPIRT LEQLDVARAP
     MALHDEIVVA TAARSFLHHQ VRAMVGTLML AGCRRLAADD VAEILASGDK SRCGPLAPAA
     GLTFVGVDYD PGK
//
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