ID TRUA_METRJ Reviewed; 253 AA.
AC B1M6N9;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2008, sequence version 1.
DT 01-MAY-2013, entry version 39.
DE RecName: Full=tRNA pseudouridine synthase A;
DE EC=5.4.99.12;
DE AltName: Full=tRNA pseudouridine(38-40) synthase;
DE AltName: Full=tRNA pseudouridylate synthase I;
DE AltName: Full=tRNA-uridine isomerase I;
GN Name=truA; OrderedLocusNames=Mrad2831_3148;
OS Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM
OS 2831).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC Methylobacteriaceae; Methylobacterium.
OX NCBI_TaxID=426355;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27329 / DSM 1819 / JCM 2831;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E.,
RA Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T.,
RA Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Mikhailova N., Marx C.J., Richardson P.;
RT "Complete sequence of chromosome of Methylobacterium radiotolerans JCM
RT 2831.";
RL Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in
CC the anticodon stem and loop of transfer RNAs (By similarity).
CC -!- CATALYTIC ACTIVITY: tRNA uridine(38-40) = tRNA pseudouridine(38-
CC 40).
CC -!- SUBUNIT: Homodimer (By similarity).
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA
CC family.
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DR EMBL; CP001001; ACB25130.1; -; Genomic_DNA.
DR RefSeq; YP_001755813.1; NC_010505.1.
DR ProteinModelPortal; B1M6N9; -.
DR STRING; 426355.Mrad2831_3148; -.
DR EnsemblBacteria; ACB25130; ACB25130; Mrad2831_3148.
DR GeneID; 6139195; -.
DR KEGG; mrd:Mrad2831_3148; -.
DR PATRIC; 22574674; VBIMetRad70578_3218.
DR eggNOG; COG0101; -.
DR HOGENOM; HOG000248672; -.
DR KO; K06173; -.
DR OMA; RHYRYRI; -.
DR ProtClustDB; PRK00021; -.
DR BioCyc; MRAD426355:GJB5-3185-MONOMER; -.
DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:HAMAP.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:HAMAP.
DR Gene3D; 3.30.70.580; -; 1.
DR Gene3D; 3.30.70.660; -; 1.
DR HAMAP; MF_00171; TruA; 1; -.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR020094; PsdUridine_synth_TruA_N.
DR PANTHER; PTHR11142; PTHR11142; 1.
DR Pfam; PF01416; PseudoU_synth_1; 2.
DR PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR SUPFAM; SSF55120; PsdUridine_synth_cat_dom; 1.
DR TIGRFAMs; TIGR00071; hisT_truA; 1.
PE 3: Inferred from homology;
KW Complete proteome; Isomerase; tRNA processing.
FT CHAIN 1 253 tRNA pseudouridine synthase A.
FT /FTId=PRO_1000097762.
FT ACT_SITE 52 52 Nucleophile (By similarity).
FT BINDING 111 111 Substrate (By similarity).
SQ SEQUENCE 253 AA; 27832 MW; EBF81A6552FC176C CRC64;
MPRYKLVIEY DGSPFCGWQR QADDPTVQGA IEAAVTRFSG ENARLTCAGR TDAGVHAIHQ
VAHLDLAKDW RTDTVRDALN AHLRPQPVAI LSAETVPDSF DARHSAIRRH YRYRILNRRS
PAALTRAHVW HVPWPLDADL MHDAAQRLVG RHDFSAFRAA ECQANSPIRT LEQLDVARAP
MALHDEIVVA TAARSFLHHQ VRAMVGTLML AGCRRLAADD VAEILASGDK SRCGPLAPAA
GLTFVGVDYD PGK
//
