ID TRUA_RHOS5 Reviewed; 257 AA.
AC A4WPU4;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 1.
DT 01-MAY-2013, entry version 44.
DE RecName: Full=tRNA pseudouridine synthase A;
DE EC=5.4.99.12;
DE AltName: Full=tRNA pseudouridine(38-40) synthase;
DE AltName: Full=tRNA pseudouridylate synthase I;
DE AltName: Full=tRNA-uridine isomerase I;
GN Name=truA; OrderedLocusNames=Rsph17025_0502;
OS Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Rhodobacter.
OX NCBI_TaxID=349102;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 17025 / ATH 2.4.3;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Richardson P.,
RA Mackenzie C., Choudhary M., Donohue T.J., Kaplan S.;
RT "Complete sequence of chromosome of Rhodobacter sphaeroides ATCC
RT 17025.";
RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in
CC the anticodon stem and loop of transfer RNAs (By similarity).
CC -!- CATALYTIC ACTIVITY: tRNA uridine(38-40) = tRNA pseudouridine(38-
CC 40).
CC -!- SUBUNIT: Homodimer (By similarity).
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA
CC family.
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DR EMBL; CP000661; ABP69408.1; -; Genomic_DNA.
DR RefSeq; YP_001166713.1; NC_009428.1.
DR ProteinModelPortal; A4WPU4; -.
DR STRING; 349102.Rsph17025_0502; -.
DR EnsemblBacteria; ABP69408; ABP69408; Rsph17025_0502.
DR GeneID; 5082861; -.
DR KEGG; rsq:Rsph17025_0502; -.
DR PATRIC; 23159136; VBIRhoSph94549_0509.
DR eggNOG; COG0101; -.
DR HOGENOM; HOG000248674; -.
DR KO; K06173; -.
DR OMA; RHYRYRI; -.
DR ProtClustDB; PRK00021; -.
DR BioCyc; RSPH349102:GHE1-1831-MONOMER; -.
DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:HAMAP.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:HAMAP.
DR Gene3D; 3.30.70.580; -; 1.
DR Gene3D; 3.30.70.660; -; 1.
DR HAMAP; MF_00171; TruA; 1; -.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR020094; PsdUridine_synth_TruA_N.
DR PANTHER; PTHR11142; PTHR11142; 1.
DR Pfam; PF01416; PseudoU_synth_1; 2.
DR PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR SUPFAM; SSF55120; PsdUridine_synth_cat_dom; 1.
DR TIGRFAMs; TIGR00071; hisT_truA; 1.
PE 3: Inferred from homology;
KW Complete proteome; Isomerase; tRNA processing.
FT CHAIN 1 257 tRNA pseudouridine synthase A.
FT /FTId=PRO_1000017155.
FT ACT_SITE 52 52 Nucleophile (By similarity).
FT BINDING 111 111 Substrate (By similarity).
SQ SEQUENCE 257 AA; 27915 MW; 919CC4274A470066 CRC64;
MPRYALKIEY DGAPFAGWQR QAVLPSVQGA IEAALGRLEP GPHTIAAAGR TDTGVHATGQ
VAHCDLGKDW DPFRLAGALN AHLKPLPVAV VAAARVGEDF HARFSAIERR YLFRLLARRA
PEVHDRGRVW RVPHPLDGAA MREAAAHLVG RHDFTTFRAI GCQAASPVKT LDELTLETVE
VPNGVEYRFR LRARSFLHNQ VRSIVGTLER VGAGAWAPDR VKEALEARDR AACGPVCPPQ
GLYLTGVGYP ADPFAAT
//
