ID TRUB_TOLAT Reviewed; 324 AA.
AC C4L8X2;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 1.
DT 29-MAY-2013, entry version 27.
DE RecName: Full=tRNA pseudouridine synthase B;
DE EC=5.4.99.25;
DE AltName: Full=tRNA pseudouridine(55) synthase;
DE Short=Psi55 synthase;
DE AltName: Full=tRNA pseudouridylate synthase;
DE AltName: Full=tRNA-uridine isomerase;
GN Name=truB; OrderedLocusNames=Tola_2244;
OS Tolumonas auensis (strain DSM 9187 / TA4).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC Aeromonadaceae; Tolumonas.
OX NCBI_TaxID=595494;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 9187 / TA4;
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T.,
RA Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N.,
RA Mikhailova N., Spring S., Beller H.;
RT "Complete sequence of Tolumonas auensis DSM 9187.";
RL Submitted (MAY-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-
CC 55 in the psi GC loop of transfer RNAs (By similarity).
CC -!- CATALYTIC ACTIVITY: tRNA uridine(55) = tRNA pseudouridine(55).
CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family.
CC Type 1 subfamily.
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DR EMBL; CP001616; ACQ93842.1; -; Genomic_DNA.
DR RefSeq; YP_002893428.1; NC_012691.1.
DR STRING; 595494.Tola_2244; -.
DR EnsemblBacteria; ACQ93842; ACQ93842; Tola_2244.
DR GeneID; 7886008; -.
DR KEGG; tau:Tola_2244; -.
DR PATRIC; 23981133; VBITolAue42623_2228.
DR eggNOG; COG0130; -.
DR HOGENOM; HOG000231223; -.
DR KO; K03177; -.
DR ProtClustDB; CLSK2517064; -.
DR BioCyc; TAUE595494:GHEF-2311-MONOMER; -.
DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:HAMAP.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:HAMAP.
DR Gene3D; 2.30.130.10; -; 1.
DR HAMAP; MF_01080; TruB_bact; 1; -.
DR InterPro; IPR002501; PsdUridine_synth.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom.
DR InterPro; IPR015947; PUA-like_domain.
DR InterPro; IPR014780; tRNA_psdUridine_synth_TruB.
DR InterPro; IPR015240; tRNA_sdUridine_synth_fam1_C.
DR PANTHER; PTHR13767; PTHR13767; 1.
DR Pfam; PF09157; TruB-C_2; 1.
DR Pfam; PF01509; TruB_N; 1.
DR SUPFAM; SSF55120; PsdUridine_synth_cat_dom; 1.
DR SUPFAM; SSF88697; PUA-like; 1.
DR TIGRFAMs; TIGR00431; TruB; 1.
PE 3: Inferred from homology;
KW Complete proteome; Isomerase; tRNA processing.
FT CHAIN 1 324 tRNA pseudouridine synthase B.
FT /FTId=PRO_1000213509.
FT ACT_SITE 49 49 Nucleophile (By similarity).
SQ SEQUENCE 324 AA; 35870 MW; 1F1745403CC12588 CRC64;
MSRRRRFKGR DVHGIILLDK PGGITSNDAL QQVKRIYNAA KAGHTGALDP LATGMLPICF
GEATKFSQFL LDADKRYQVT ARLGVRTDTS DSEGSVVSVR PVDVSEDQLA QALDKFRGDI
MQVPSMFSAL KHQGRPLYEY AREGIEIERE ARPITIYSLE LKAFSGEEIS LEVHCSKGTY
IRSLIDDLGE LLGCGAHVIQ LRRTQVACYP NDKMLNLEKL NEILDECREQ GVPPRERLDQ
YLLPMDSAVY SLPEVNMPPV LAAYVTQGQA VMVPHAPIEG FVRMTVGPEA EFIGVGEIDD
DGKVAPRRLV RIGGDMPDAE EQAE
//
