ID TYSY_SCHPO Reviewed; 625 AA.
AC Q9UTI7;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-APR-2013, entry version 79.
DE RecName: Full=Probable thymidylate synthase;
DE Short=TS;
DE Short=TSase;
DE EC=2.1.1.45;
GN ORFNames=SPAC15E1.04;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales;
OC Schizosaccharomycetaceae; Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M.,
RA Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A.,
RA Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G.,
RA Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K.,
RA James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J.,
RA Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C.,
RA Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E.,
RA Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S.,
RA Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K.,
RA Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S.,
RA Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B.,
RA Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S.,
RA Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D.,
RA Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R.,
RA Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B.,
RA Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S.,
RA Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M.,
RA Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G.,
RA Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J.,
RA Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L.,
RA Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J.,
RA Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the
RT fission yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Required for both nuclear and mitochondrial DNA
CC synthesis (By similarity).
CC -!- CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + dUMP =
CC dihydrofolate + dTMP.
CC -!- PATHWAY: Pyrimidine metabolism; dTTP biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: In the N-terminal section; belongs to the HFCD (homo-
CC oligomeric flavin containing Cys decarboxylase) superfamily.
CC -!- SIMILARITY: In the C-terminal section; belongs to the thymidylate
CC synthase family.
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DR EMBL; CU329670; CAB52423.1; -; Genomic_DNA.
DR PIR; T37719; T37719.
DR RefSeq; NP_594305.1; NM_001019728.2.
DR HSSP; P13100; 1CI7.
DR ProteinModelPortal; Q9UTI7; -.
DR SMR; Q9UTI7; 327-625.
DR STRING; 4896.SPAC15E1.04-1; -.
DR EnsemblFungi; SPAC15E1.04.1; SPAC15E1.04.1:pep; SPAC15E1.04.
DR GeneID; 2542801; -.
DR KEGG; spo:SPAC15E1.04; -.
DR PomBase; SPAC15E1.04; -.
DR eggNOG; COG0452; -.
DR KO; K01598; -.
DR OrthoDB; EOG418FX9; -.
DR UniPathway; UPA00575; -.
DR NextBio; 20803844; -.
DR GO; GO:0005829; C:cytosol; IDA:PomBase.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; ISM:PomBase.
DR GO; GO:0004799; F:thymidylate synthase activity; ISS:PomBase.
DR GO; GO:0006231; P:dTMP biosynthetic process; ISS:PomBase.
DR GO; GO:0006235; P:dTTP biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.30.572.10; -; 1.
DR Gene3D; 3.40.50.1950; -; 1.
DR InterPro; IPR003382; Flavoprotein.
DR InterPro; IPR023451; Thymidate_synth/dCMP_Mease.
DR InterPro; IPR000398; Thymidylate_synthase.
DR InterPro; IPR020940; Thymidylate_synthase_AS.
DR Pfam; PF02441; Flavoprotein; 1.
DR Pfam; PF00303; Thymidylat_synt; 1.
DR PRINTS; PR00108; THYMDSNTHASE.
DR SUPFAM; SSF52507; Flavoprotein; 1.
DR SUPFAM; SSF55831; Thymidylat_synth_C; 1.
DR TIGRFAMs; TIGR03284; thym_sym; 1.
DR PROSITE; PS00091; THYMIDYLATE_SYNTHASE; 1.
PE 3: Inferred from homology;
KW Complete proteome; Cytoplasm; Methyltransferase;
KW Nucleotide biosynthesis; Reference proteome; Transferase.
FT CHAIN 1 625 Probable thymidylate synthase.
FT /FTId=PRO_0000310825.
FT COMPBIAS 229 255 Asp-rich.
FT ACT_SITE 497 497 By similarity.
SQ SEQUENCE 625 AA; 69893 MW; B3BC6034BF26F61E CRC64;
MSQPLHARFA TRAVKNPMIL EKERQLTDSK YHILVAATGS VAAIKLTLIV KSLLTYKGVD
VQVVLTDPAR NFVEKEDLTA LGVNVYNNAD DWKNWDGLEC PITHIELRRW AHLLLIAPLS
ANTMAKMANG LCDNLLTSLI RAWAPLKPIL LAPAMNTLMW TNPITQEHLS AISRIYKNSE
FIMPIEKVLA CGDIGMGGMA EWRNIVGRVA DKLQLEQKSV LPNAVKNIDG QDDDSSEQTA
AFEEYDDDDD DDVDDNEQSN SMIETSANAD ITPKASLLPS TTESSISKDH ETSQAPLGSE
SVDTQASENV TTKPEPPVPF TSSEYRNTEE EQYLNLIRYI LENGQSRPDR TGTGTRSVFA
PPQLRFSLRN NTLPLLTTKR VFLRGVLEEL LWFIHGDTNA NHLSEKGIHI WDGNGSREFL
DSRGLTDRKV GDLGPIYGFQ WRHFGAQYVD CDTDYTNKGV DQLAQVISTL KLNPYDRRII
LSAWNPLAIP EMALPPCHIF CQFYVSEPCK PGGKPQLSSM MYQRSADMGL GVPFNIASYS
LLTHMIAHMC GYEAAEFVHV MGDCHIYNDH LEALQTQLER VPKAFPKLFF KRDAKDIGSI
DSFSVDDFAV EGYNPYGPIK MKMSV
//
