ID Y2481_CLOTE Reviewed; 460 AA.
AC Q891A0;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 01-MAY-2013, entry version 62.
DE RecName: Full=Uncharacterized RNA methyltransferase CTC_02481;
DE EC=2.1.1.-;
GN OrderedLocusNames=CTC_02481;
OS Clostridium tetani (strain Massachusetts / E88).
OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=212717;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Massachusetts / E88;
RX PubMed=12552129; DOI=10.1073/pnas.0335853100;
RA Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H.,
RA Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A.,
RA Gottschalk G.;
RT "The genome sequence of Clostridium tetani, the causative agent of
RT tetanus disease.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003).
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. RNA M5U
CC methyltransferase family.
CC -!- SIMILARITY: Contains 1 TRAM domain.
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DR EMBL; AE015927; AAO36945.1; -; Genomic_DNA.
DR RefSeq; NP_783008.1; NC_004557.1.
DR ProteinModelPortal; Q891A0; -.
DR STRING; 212717.CTC02481; -.
DR EnsemblBacteria; AAO36945; AAO36945; CTC_02481.
DR GeneID; 1060641; -.
DR KEGG; ctc:CTC02481; -.
DR PATRIC; 19513083; VBICloTet101274_2572.
DR eggNOG; COG2265; -.
DR KO; K03215; -.
DR OMA; FFQVNPV; -.
DR BioCyc; CTET212717:GJAM-2292-MONOMER; -.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0001510; P:RNA methylation; IEA:GOC.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR001566; 23S_rRNA_MeTrfase_RlmD.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR002792; TRAM_dom.
DR InterPro; IPR010280; U5_MeTrfase.
DR Pfam; PF01938; TRAM; 1.
DR Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR SUPFAM; SSF50249; Nucleic_acid_OB; 1.
DR TIGRFAMs; TIGR00479; rumA; 1.
DR PROSITE; PS50926; TRAM; 1.
DR PROSITE; PS01230; TRMA_1; 1.
DR PROSITE; PS01231; TRMA_2; FALSE_NEG.
PE 3: Inferred from homology;
KW 4Fe-4S; Complete proteome; Iron; Iron-sulfur; Metal-binding;
KW Methyltransferase; S-adenosyl-L-methionine; Transferase.
FT CHAIN 1 460 Uncharacterized RNA methyltransferase
FT CTC_02481.
FT /FTId=PRO_0000161973.
FT DOMAIN 6 64 TRAM.
FT ACT_SITE 412 412 Nucleophile (By similarity).
FT METAL 77 77 Iron-sulfur (4Fe-4S) (By similarity).
FT METAL 83 83 Iron-sulfur (4Fe-4S) (By similarity).
FT METAL 86 86 Iron-sulfur (4Fe-4S) (By similarity).
FT METAL 163 163 Iron-sulfur (4Fe-4S) (By similarity).
FT BINDING 287 287 S-adenosyl-L-methionine (By similarity).
FT BINDING 316 316 S-adenosyl-L-methionine; via carbonyl
FT oxygen (By similarity).
FT BINDING 337 337 S-adenosyl-L-methionine (By similarity).
FT BINDING 385 385 S-adenosyl-L-methionine (By similarity).
SQ SEQUENCE 460 AA; 51947 MW; 8C9D93994BADE623 CRC64;
MKKDIPVKKN STYNLYITGM GTKGEGIGKI NNFTIFVTGA ILGEEVEVNI IKVNKNYAVG
KLLNIITPSA NRVEPPCDIY TKCGGCQLQH MSYKEQLNFK RQKVKDALLR LGGIDVEVEE
VLGMENPYRY RNKVQLPIGK EKGKVNIGFY APRSHNIIDL KSCFIQDEKA DKIIKILKEW
IEKFNVSIYD EKEHKGNLRH IMVRTAFRTG QIMIVLVTKD KKLPHKEELI NKLTEDLEGV
VSIIQNMNSQ KTNVVLGKES IVLWGKDKII DYIGNFKFAI TPLSFFQVNP IQTEVLYNKA
LEYADLKGDE VVFDAYCGTG TISLFLSQKA KKVYGVEIVN EAIESAKLNA RENNVDNVDF
IVGESEQIIP ELIEKGIKAD VVVVDPPRKG CEKSLLEAMA KMAPEKIVYV SCDPATLARD
LGVLEELGYK TMKVQPVDMF SNTYHVETII LMTYYGDKKK
//
