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Entry
spo03030                    Pathway                                

Name
DNA replication - Schizosaccharomyces pombe (fission yeast)
Description
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Class
Genetic Information Processing; Replication and repair
BRITE hierarchy
Pathway map
DNA replication
spo03030

All organismsOrtholog table
Module
DNA polymerase alpha / primase complex [PATH:spo03030]
DNA polymerase delta complex [PATH:spo03030]
DNA polymerase epsilon complex [PATH:spo03030]
MCM complex [PATH:spo03030]
RPA complex [PATH:spo03030]
RF-C complex [PATH:spo03030]
Organism
Schizosaccharomyces pombe (fission yeast) [GN:spo]
Gene
rnh1; ribonuclease H Rnh1 (predicted) [KO:K03469] [EC:3.1.26.4]
ssb1; DNA replication factor A subunit Ssb1 [KO:K07466]
pcn1; DNA polymerase delta processivity factor [KO:K04802]
dna2; DNA replication endonuclease-helicase Dna2 [KO:K10742] [EC:3.6.4.12]
rad2; FEN-1 endonuclease Rad2 [KO:K04799] [EC:3.-.-.-]
cdc17; ATP-dependent DNA ligase Cdc17 [KO:K10747] [EC:6.5.1.7 6.5.1.6 6.5.1.1]
adl1; DNA ligase (predicted) [KO:K10747] [EC:6.5.1.7 6.5.1.6 6.5.1.1]
pol1; DNA polymerase alpha catalytic subunit [KO:K02320] [EC:2.7.7.7]
spb70; DNA polymerase alpha B-subunit [KO:K02321]
spp1; DNA primase catalytic subunit Spp1 [KO:K02684] [EC:2.7.7.-]
spp2; DNA primase large subunit Spp2 [KO:K02685] [EC:2.7.7.-]
cdc6; DNA polymerase delta catalytic subunit Cdc6 [KO:K02327] [EC:2.7.7.7]
cdc1; DNA polymerase delta small subunit Cdc1 [KO:K02328]
cdc27; DNA polymerase delta subunit Cdc27 [KO:K03504]
cdm1; DNA polymerase delta subunit Cdm1 [KO:K03505]
cdc20; DNA polymerase epsilon catalytic subunit Pol2 [KO:K02324] [EC:2.7.7.7]
dpb2; DNA polymerase epsilon catalytic subunit B, Dpb2 [KO:K02325] [EC:2.7.7.7]
dpb4; DNA polymerase epsilon subunit Dpb4 [KO:K02326] [EC:2.7.7.7]
histone-like transcription factor family (CBF/NF-Y) [KO:K03506] [EC:2.7.7.7]
mcm2; MCM complex subunit Mcm2 [KO:K02540] [EC:3.6.4.12]
mcm3; MCM complex subunit Mcm3 [KO:K02541] [EC:3.6.4.12]
mcm4; MCM complex subunit Mcm4/Cdc21 [KO:K02212] [EC:3.6.4.12]
mcm5; MCM complex subunit Mcm5 [KO:K02209] [EC:3.6.4.12]
mcm6; MCM complex subunit Mcm6 [KO:K02542] [EC:3.6.4.12]
mcm7; MCM complex subunit Mcm7 [KO:K02210] [EC:3.6.4.12]
ssb2; single-stranded DNA binding protein Ssb2 [KO:K10739]
ssb3; DNA replication factor A subunit Ssb3 [KO:K10740]
rfc1; DNA replication factor C complex subunit Rfc1 [KO:K10754]
rfc2; DNA replication factor C complex subunit Rfc2 [KO:K10755]
rfc4; DNA replication factor C complex subunit Rfc4 (predicted) [KO:K10755]
rfc3; DNA replication factor C complex subunit Rfc3 [KO:K10756]
rfc5; DNA replication factor C complex subunit Rfc5 (predicted) [KO:K10756]
rnh201; ribonuclease H2 complex subunit Rnh201 (predicted) [KO:K10743] [EC:3.1.26.4]
ribonuclease H2 complex subunit (predicted) [KO:K10744]
ribonuclease H2 complex subunit (predicted) [KO:K10745]
Reference
PMID:8087839
  Authors
Stillman B.
  Title
Smart machines at the DNA replication fork.
  Journal
Cell 78:725-8 (1994)
DOI:10.1016/S0092-8674(94)90362-X
Reference
PMID:9759502
  Authors
Waga S, Stillman B.
  Title
The DNA replication fork in eukaryotic cells.
  Journal
Annu Rev Biochem 67:721-51 (1998)
DOI:10.1146/annurev.biochem.67.1.721
KO pathway
 

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