Database: SPTrEMBLEntry: XYLG_PSEPU
LinkDB: XYLG_PSEPU
Original site: XYLG_PSEPU
ID XYLG_PSEPU Reviewed; 486 AA.
AC P23105;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1991, sequence version 1.
DT 19-JAN-2010, entry version 64.
DE RecName: Full=2-hydroxymuconic semialdehyde dehydrogenase;
DE Short=HMSD;
DE EC=1.2.1.-;
GN Name=xylG;
OS Pseudomonas putida.
OG Plasmid TOL pWW0.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=303;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=92167811; PubMed=1791759;
RX DOI=10.1111/j.1365-2958.1991.tb02091.x;
RA Horn J.M., Harayama S., Timmis K.N.;
RT "DNA sequence determination of the TOL plasmid (pWWO) xylGFJ genes of
RT Pseudomonas putida: implications for the evolution of aromatic
RT catabolism.";
RL Mol. Microbiol. 5:2459-2474(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PAO;
RX MEDLINE=92317087; PubMed=1339433;
RA Steele M.I., Lorenz D., Hatter K., Park A., Sokatch J.R.;
RT "Characterization of the mmsAB operon of Pseudomonas aeruginosa PAO
RT encoding methylmalonate-semialdehyde dehydrogenase and 3-
RT hydroxyisobutyrate dehydrogenase.";
RL J. Biol. Chem. 267:13585-13592(1992).
RN [3]
RP PROTEIN SEQUENCE OF 1-15.
RX MEDLINE=95173094; PubMed=7868591;
RA Inoue J., Shaw J.P., Rekik M., Harayama S.;
RT "Overlapping substrate specificities of benzaldehyde dehydrogenase
RT (the xylC gene product) and 2-hydroxymuconic semialdehyde
RT dehydrogenase (the xylG gene product) encoded by TOL plasmid pWW0 of
RT Pseudomonas putida.";
RL J. Bacteriol. 177:1196-1201(1995).
CC -!- FUNCTION: 2-hydroxymuconic acid semialdehyde can be converted to
CC 2-hydroxypent-2,4-dienoate either directly by the action of 2-
CC hydroxymuconic semialdehyde hydrolase (HMSH) or by the action of
CC three sequential enzymes, the first of which is HMSD. Can oxidize
CC not only 2-hydroxymuconic semialdehyde and its analogs but also
CC benzaldehyde and its analogs.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 8.3 for the oxidation of 2-hydroxymuconic
CC semialdehyde, and 9.6 for that of benzaldehyde;
CC -!- PATHWAY: Aromatic compound metabolism; benzoate degradation via
CC hydroxylation.
CC -!- SUBUNIT: Homodimer.
CC -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
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DR EMBL; M64747; AAA26053.1; -; Genomic_DNA.
DR PIR; E42902; E42902.
DR RefSeq; NP_542865.1; -.
DR SMR; P23105; 4-486.
DR GeneID; 1218748; -.
DR BioCyc; MetaCyc:MONOMER-3402; -.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR InterPro; IPR016161; Ald_DH/histidinol_DH.
DR InterPro; IPR016160; Ald_DH_CS.
DR InterPro; IPR016162; Ald_DH_N.
DR InterPro; IPR015590; Aldehyde_DH.
DR InterPro; IPR017628; OHmuconic_semiald_DH.
DR Gene3D; G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.
DR PANTHER; PTHR11699; Aldehyde_dehyd; 1.
DR Pfam; PF00171; Aldedh; 1.
DR PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; 1.
DR PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE 1: Evidence at protein level;
KW Aromatic hydrocarbons catabolism; Direct protein sequencing; NAD;
KW Oxidoreductase; Plasmid.
FT CHAIN 1 486 2-hydroxymuconic semialdehyde
FT dehydrogenase.
FT /FTId=PRO_0000056594.
FT ACT_SITE 254 254 By similarity.
FT ACT_SITE 288 288 By similarity.
FT CONFLICT 11 12 EL -> AF (in Ref. 3; AA sequence).
SQ SEQUENCE 486 AA; 51761 MW; 854303F121B2FFBD CRC64;
MKEIKHFISG ELVGSASGKL FDNVSPANGQ VIGRVHEAGR AEVDAAVRAA RAALKGPWGK
MTVAERAEIL HRVADGITAR FGEFLEARMP GHRQAEVAGQ PHRHSARRAN FKVFADLLKN
VANEAFEMAT PDGAGALNYG VRRPKGVIGV ISPWNLPLLL MTWKVGPALA CGNCVVVKPS
EETPLTATLL GEVMQAAGVP AGVYNVVHGF GGDSAGAFLT EHPDVDAYTF TGETGTGETI
MRAAAKGVRQ VSLELGGKNA GIVFADCDMD KAIEGTLRSA FANCGQVCLG TERVYVERPI
FDAFVARLKA GAEALKIGEP NDPEANFGPL ISHKPREKVP SYYQQAVDDG ATVVTGGGVP
EMPAHLAGGA WVQPTIWTGL ADDSAVVTEE IFGPCCHIRP FDSEEEAIEL ANSLPYGLAS
AIWTENVRRA HRVAGQIEAG IVWVNSWFLR DLRTAFGGSK QSGIGREGGV HSLEFYTELK
NICVKL
//
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