ID A0A060NL41_9BURK Unreviewed; 579 AA.
AC A0A060NL41;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 24-JAN-2024, entry version 32.
DE SubName: Full=Alkaline phosphatase {ECO:0000313|EMBL:BAO82462.1};
GN ORFNames=SMCB_0234 {ECO:0000313|EMBL:BAO82462.1};
OS Serpentinimonas maccroryi.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Serpentinimonas.
OX NCBI_TaxID=1458426 {ECO:0000313|EMBL:BAO82462.1, ECO:0000313|Proteomes:UP000066014};
RN [1] {ECO:0000313|EMBL:BAO82462.1, ECO:0000313|Proteomes:UP000066014}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B1 {ECO:0000313|EMBL:BAO82462.1,
RC ECO:0000313|Proteomes:UP000066014};
RX PubMed=24845058; DOI=10.1038/ncomms4900;
RA Suzuki S., Kuenen J.G., Schipper K., van der Velde S., Ishii S., Wu A.,
RA Sorokin D.Y., Tenney A., Meng X.Y., Morrill P.L., Kamagata Y., Muyzer G.,
RA Nealson K.H.;
RT "Physiological and genomic features of highly alkaliphilic hydrogen-
RT utilizing Betaproteobacteria from a continental serpentinizing site.";
RL Nat. Commun. 5:3900-3900(2014).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR601952-2};
CC Note=Binds 1 Mg(2+) ion. {ECO:0000256|PIRSR:PIRSR601952-2};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PIRSR:PIRSR601952-2};
CC Note=Binds 2 Zn(2+) ions. {ECO:0000256|PIRSR:PIRSR601952-2};
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DR EMBL; AP014569; BAO82462.1; -; Genomic_DNA.
DR RefSeq; WP_052468360.1; NZ_JAAKER010000013.1.
DR AlphaFoldDB; A0A060NL41; -.
DR STRING; 1458426.SMCB_0234; -.
DR KEGG; cbab:SMCB_0234; -.
DR HOGENOM; CLU_008539_6_1_4; -.
DR Proteomes; UP000066014; Chromosome.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016791; F:phosphatase activity; IEA:InterPro.
DR Gene3D; 3.40.720.10; Alkaline Phosphatase, subunit A; 1.
DR InterPro; IPR001952; Alkaline_phosphatase.
DR InterPro; IPR017850; Alkaline_phosphatase_core_sf.
DR PANTHER; PTHR11596; ALKALINE PHOSPHATASE; 1.
DR PANTHER; PTHR11596:SF5; ALKALINE PHOSPHATASE; 1.
DR Pfam; PF00245; Alk_phosphatase; 1.
DR SMART; SM00098; alkPPc; 1.
DR SUPFAM; SSF53649; Alkaline phosphatase-like; 1.
PE 4: Predicted;
KW Magnesium {ECO:0000256|PIRSR:PIRSR601952-2};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601952-2};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Signal {ECO:0000256|SAM:SignalP}; Zinc {ECO:0000256|PIRSR:PIRSR601952-2}.
FT SIGNAL 1..23
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 24..579
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5001584966"
FT ACT_SITE 101
FT /note="Phosphoserine intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-1"
FT BINDING 57
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 57
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 386
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 391
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
SQ SEQUENCE 579 AA; 61782 MW; 6CDBBF64FB9B4199 CRC64;
MFNLNLRAVS AACVLALATG VAAASAASAA RAPAADPGRA AMTQAVPTGN VIFFHPDGTG
LNHWNAHRMY FHGLDGISNK DRLPHMAVYR GHMHNLINGT SHAGATTHAF GYKVDAFGSF
GMDGDGNANP PTNRAIRSLS GFRGSVMREA ANAGIPVGVV NDGHVGEPGT GAFLAEVGNR
DNWQEITRQM ILGRPGMNDT TPWVIMGGGE ADALPQGTTT VHRNVNEERR APVNSRQSLR
TDDLNLIAAW NAMGSGNLSN DPTQRDDFIV LRTRAEFERL KQALQAHPRY APRVLGLFAF
QDTFNDRNEQ DLINRGFVRA GMAAGTTGAG PQKQSRLVLW GDHDAAEPGF NPPTFAEMTE
VALTILSRAA AQRPQAAQRR FFLVAEQESI DNFGNNTNAI GMLHAMQDTD RAIGAALDFL
RRNERTLIVT AADSDAGSMT VVSGQVSGIT PPTNILPAGR APDNVASVAT NPSSAIPAGA
TGPDAAVVNN FLDGVEGRQS PMFVTEPDQF GNRMQFGIAW PGTADYFGGV LSRAAGLNAG
LLNSRFSQRF DNVDVYRLKH ATLFGRMLNY PTATEAPGR
//
