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Database: UniProt/TrEMBL
Entry: A0A075P8R7_PSEFL
LinkDB: A0A075P8R7_PSEFL
Original site: A0A075P8R7_PSEFL 
ID   A0A075P8R7_PSEFL        Unreviewed;       397 AA.
AC   A0A075P8R7;
DT   29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT   29-OCT-2014, sequence version 1.
DT   27-SEP-2017, entry version 26.
DE   RecName: Full=Elongation factor Tu {ECO:0000256|HAMAP-Rule:MF_00118, ECO:0000256|RuleBase:RU004061};
DE            Short=EF-Tu {ECO:0000256|HAMAP-Rule:MF_00118};
GN   Name=tuf {ECO:0000256|HAMAP-Rule:MF_00118,
GN   ECO:0000313|EMBL:AIG03305.1};
GN   Synonyms=tuf1 {ECO:0000313|EMBL:KIR20822.1};
GN   ORFNames=AO066_26700 {ECO:0000313|EMBL:KTB65445.1}, BFW86_25880
GN   {ECO:0000313|EMBL:OPA83980.1}, BFW91_09560
GN   {ECO:0000313|EMBL:OPB13644.1}, BTR19_14520
GN   {ECO:0000313|EMBL:OKP70395.1}, HZ99_14445
GN   {ECO:0000313|EMBL:AIG03305.1}, PFL603g_05833
GN   {ECO:0000313|EMBL:KWV71150.1}, PFLU3_37690
GN   {ECO:0000313|EMBL:KIR20822.1}, VO64_2421
GN   {ECO:0000313|EMBL:AKA82967.1};
OS   Pseudomonas fluorescens.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=294 {ECO:0000313|EMBL:AIG03305.1, ECO:0000313|Proteomes:UP000028180};
RN   [1] {ECO:0000313|EMBL:AIG03305.1, ECO:0000313|Proteomes:UP000028180}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UK4 {ECO:0000313|EMBL:AIG03305.1,
RC   ECO:0000313|Proteomes:UP000028180};
RX   PubMed=25212622;
RA   Dueholm M.S., Danielsen H.N., Nielsen P.H.;
RT   "Complete Genome Sequence of Pseudomonas sp. UK4, a Model Organism for
RT   Studies of Functional Amyloids in Pseudomonas.";
RL   Genome Announc. 2:e00898-14(2014).
RN   [2] {ECO:0000313|EMBL:AKA82967.1, ECO:0000313|Proteomes:UP000033099}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LBUM223 {ECO:0000313|EMBL:AKA82967.1,
RC   ECO:0000313|Proteomes:UP000033099};
RX   PubMed=25953163;
RA   Roquigny R., Arseneault T., Gadkar V.J., Novinscak A., Joly D.L.,
RA   Filion M.;
RT   "Complete Genome Sequence of Biocontrol Strain Pseudomonas fluorescens
RT   LBUM223.";
RL   Genome Announc. 3:0-0(2015).
RN   [3] {ECO:0000313|EMBL:KIR20822.1, ECO:0000313|Proteomes:UP000032210}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=2-79 {ECO:0000313|EMBL:KIR20822.1,
RC   ECO:0000313|Proteomes:UP000032210};
RA   Thuermer A., Daniel R.;
RT   "Genome sequence of the beneficial rhizobacterium Pseudomonas
RT   fluorescens 2-79.";
RL   Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
RN   [4] {ECO:0000313|EMBL:KWV71150.1, ECO:0000313|Proteomes:UP000063434}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ps_40 {ECO:0000313|EMBL:KWV71150.1,
RC   ECO:0000313|Proteomes:UP000063434};
RA   Andreani N.A., Cardazzo B.;
RT   "A genomic and transcriptomic approach to investigate the blue pigment
RT   phenotype in Pseudomonas fluorescens.";
RL   Submitted (MAY-2015) to the EMBL/GenBank/DDBJ databases.
RN   [5] {ECO:0000313|EMBL:KTB65445.1, ECO:0000313|Proteomes:UP000054848}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ICMP3636 {ECO:0000313|EMBL:KTB65445.1,
RC   ECO:0000313|Proteomes:UP000054848};
RA   Visnovsky S., Lu A., Panda P., Pitman A.;
RT   "Genome sequence of ICMP 3636.";
RL   Submitted (SEP-2015) to the EMBL/GenBank/DDBJ databases.
RN   [6] {ECO:0000313|EMBL:OKP70395.1, ECO:0000313|Proteomes:UP000185704}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ML11A {ECO:0000313|EMBL:OKP70395.1,
RC   ECO:0000313|Proteomes:UP000185704};
RA   Gauthier J., Charette S.J., Derome N.;
RT   "Draft Genome Sequence of Pseudomonas fluorescens ML11A, an Endogenous
RT   Strain from Brook Charr with Antagonistic Properties Against Aeromonas
RT   salmonicida subsp. salmonicida.";
RL   Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
RN   [7] {ECO:0000313|EMBL:OPA83980.1, ECO:0000313|Proteomes:UP000189866, ECO:0000313|Proteomes:UP000190869}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AH4 {ECO:0000313|EMBL:OPA83980.1,
RC   ECO:0000313|Proteomes:UP000189866}, and E24
RC   {ECO:0000313|EMBL:OPB13644.1, ECO:0000313|Proteomes:UP000190869};
RA   Okrent R.A., Manning V.A., Trippe K.M.;
RT   "Draft genome sequences of seven strains of Pseudomonas fluorescens
RT   that produce 4-formylaminooxyvinylglycine.";
RL   Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: This protein promotes the GTP-dependent binding of
CC       aminoacyl-tRNA to the A-site of ribosomes during protein
CC       biosynthesis. {ECO:0000256|HAMAP-Rule:MF_00118}.
CC   -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00118}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00118}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A
CC       subfamily. {ECO:0000256|HAMAP-Rule:MF_00118}.
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DR   EMBL; CP008896; AIG03305.1; -; Genomic_DNA.
DR   EMBL; CP011117; AKA82967.1; -; Genomic_DNA.
DR   EMBL; JXCQ01000038; KIR20822.1; -; Genomic_DNA.
DR   EMBL; LKEI01000016; KTB65445.1; -; Genomic_DNA.
DR   EMBL; LCYC01000062; KWV71150.1; -; Genomic_DNA.
DR   EMBL; MRXZ01000010; OKP70395.1; -; Genomic_DNA.
DR   EMBL; MSDE01000033; OPA83980.1; -; Genomic_DNA.
DR   EMBL; MSDI01000008; OPB13644.1; -; Genomic_DNA.
DR   RefSeq; WP_003176426.1; NZ_MSDL01000079.1.
DR   SMR; A0A075P8R7; -.
DR   EnsemblBacteria; AIG03305; AIG03305; HZ99_14445.
DR   EnsemblBacteria; AKA82967; AKA82967; VO64_2421.
DR   EnsemblBacteria; KIR20822; KIR20822; PFLU3_37690.
DR   EnsemblBacteria; KTB65445; KTB65445; AO066_26700.
DR   EnsemblBacteria; KWV71150; KWV71150; PFL603g_05833.
DR   KEGG; pfb:VO64_2421; -.
DR   KEGG; pfn:HZ99_14445; -.
DR   PATRIC; fig|294.123.peg.2906; -.
DR   eggNOG; ENOG4105CGV; Bacteria.
DR   eggNOG; COG0050; LUCA.
DR   KO; K02358; -.
DR   Proteomes; UP000028180; Chromosome.
DR   Proteomes; UP000032210; Unassembled WGS sequence.
DR   Proteomes; UP000033099; Chromosome.
DR   Proteomes; UP000054848; Unassembled WGS sequence.
DR   Proteomes; UP000063434; Unassembled WGS sequence.
DR   Proteomes; UP000185704; Unassembled WGS sequence.
DR   Proteomes; UP000189866; Unassembled WGS sequence.
DR   Proteomes; UP000190869; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-UniRule.
DR   CDD; cd03697; EFTU_II; 1.
DR   HAMAP; MF_00118_B; EF_Tu_B; 1.
DR   InterPro; IPR004161; EFTu-like_2.
DR   InterPro; IPR033720; EFTU_2.
DR   InterPro; IPR031157; G_TR_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; TF_GTP-bd_dom.
DR   InterPro; IPR009000; Transl_B-barrel.
DR   InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR   InterPro; IPR004541; Transl_elong_EFTu/EF1A_bac/org.
DR   InterPro; IPR004160; Transl_elong_EFTu/EF1A_C.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF03143; GTP_EFTU_D3; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50447; SSF50447; 1.
DR   SUPFAM; SSF50465; SSF50465; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00485; EF-Tu; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS00301; G_TR_1; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000028180,
KW   ECO:0000313|Proteomes:UP000032210, ECO:0000313|Proteomes:UP000033099,
KW   ECO:0000313|Proteomes:UP000054848};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00118};
KW   Elongation factor {ECO:0000256|HAMAP-Rule:MF_00118,
KW   ECO:0000313|EMBL:AIG03305.1};
KW   GTP-binding {ECO:0000256|HAMAP-Rule:MF_00118};
KW   Hydrolase {ECO:0000313|EMBL:AIG03305.1};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00118};
KW   Protein biosynthesis {ECO:0000256|HAMAP-Rule:MF_00118}.
FT   DOMAIN       10    207       Tr-type G. {ECO:0000259|PROSITE:PS51722}.
FT   NP_BIND      19     26       GTP. {ECO:0000256|HAMAP-Rule:MF_00118}.
FT   NP_BIND      81     85       GTP. {ECO:0000256|HAMAP-Rule:MF_00118}.
FT   NP_BIND     136    139       GTP. {ECO:0000256|HAMAP-Rule:MF_00118}.
SQ   SEQUENCE   397 AA;  43564 MW;  6AE27F6A62214BD9 CRC64;
     MAKEKFDRSL PHVNVGTIGH VDHGKTTLTA ALTRVCSEVF GSAIVDFDKI DSAPEEKARG
     ITINTAHVEY NSLIRHYAHV DCPGHADYVK NMITGAAQMD GAILVCSAAD GPMPQTREHI
     LLSRQVGVPY IVVYLNKADL VDDAELLELV EMEVRDLLST YDFPGDDTPI IIGSARMALE
     GKDDNEMGTT SVRKLVETLD SYIPDPVRVI DKPFLMPIED VFSISGRGTV VTGRIERGIV
     KVQDPLEIVG LRDTTVTTCT GVEMFRKLLD EGRAGENCGV LLRGTKRDDV ERGQVLVKPG
     SVKPHTKFEA EVYVLSKEEG GRHTPFFKGY RPQFYFRTTD VTGNCELPEG VEMVMPGDNI
     KMVVTLIKTI AMEDGLRFAI REGGRTVGAG VVAKIIE
//
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