ID A0A089LE41_9BACL Unreviewed; 692 AA.
AC A0A089LE41;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 24-JAN-2024, entry version 29.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
DE Short=Beta-gal {ECO:0000256|PIRNR:PIRNR001084};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
GN ORFNames=PBOR_20990 {ECO:0000313|EMBL:AIQ59132.1};
OS Paenibacillus borealis.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=160799 {ECO:0000313|EMBL:AIQ59132.1, ECO:0000313|Proteomes:UP000029518};
RN [1] {ECO:0000313|EMBL:AIQ59132.1, ECO:0000313|Proteomes:UP000029518}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 13188 {ECO:0000313|EMBL:AIQ59132.1,
RC ECO:0000313|Proteomes:UP000029518};
RA den Bakker H.C., Tsai Y.-C.Y.-C., Martin N., Korlach J., Wiedmann M.;
RT "Comparative genomics of the Paenibacillus odorifer group.";
RL Submitted (AUG-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|PIRNR:PIRNR001084};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 42 family.
CC {ECO:0000256|ARBA:ARBA00005940, ECO:0000256|PIRNR:PIRNR001084}.
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DR EMBL; CP009285; AIQ59132.1; -; Genomic_DNA.
DR RefSeq; WP_042214733.1; NZ_CP009285.1.
DR AlphaFoldDB; A0A089LE41; -.
DR KEGG; pbd:PBOR_20990; -.
DR HOGENOM; CLU_012430_1_1_9; -.
DR OrthoDB; 9800974at2; -.
DR Proteomes; UP000029518; Chromosome.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006012; P:galactose metabolic process; IEA:InterPro.
DR CDD; cd03143; A4_beta-galactosidase_middle_domain; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013739; Beta_galactosidase_C.
DR InterPro; IPR013738; Beta_galactosidase_Trimer.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR003476; Glyco_hydro_42.
DR InterPro; IPR013529; Glyco_hydro_42_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR36447; BETA-GALACTOSIDASE GANA; 1.
DR PANTHER; PTHR36447:SF1; BETA-GALACTOSIDASE GANA; 1.
DR Pfam; PF02449; Glyco_hydro_42; 1.
DR Pfam; PF08533; Glyco_hydro_42C; 1.
DR Pfam; PF08532; Glyco_hydro_42M; 1.
DR PIRSF; PIRSF001084; B-galactosidase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR001084};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR001084};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001084-3};
KW Zinc {ECO:0000256|PIRSR:PIRSR001084-3}.
FT DOMAIN 20..393
FT /note="Glycoside hydrolase family 42 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02449"
FT DOMAIN 405..618
FT /note="Beta-galactosidase trimerisation"
FT /evidence="ECO:0000259|Pfam:PF08532"
FT DOMAIN 629..679
FT /note="Beta-galactosidase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF08533"
FT ACT_SITE 158
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT ACT_SITE 316
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT BINDING 119
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 123
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-3"
FT BINDING 157
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 163
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-3"
FT BINDING 165
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-3"
FT BINDING 168
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-3"
FT BINDING 324
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
SQ SEQUENCE 692 AA; 78243 MW; EE9D97A182290FCE CRC64;
MSSKVPAISS KAPVMLHGAD YNPEQWLKYP EVLKEDIRMM KLAGCNVMSV GIFSWVSLER
EEGVFTFEWL DQLLDTFKEN GVYAFLATPS GARPAWMSEK YPEVLRVERN RVRNLHGVRH
NHCFTSPVYR EKTALMNSKL AERYAHHPAV IGWHISNEFG GECHCEYCQN AFREWLKVKY
KGDLDELNHA WWATFWSHTY TSWEQIESPA PHGETQVHGL NLDWRRFVSD QTINFCQHEI
DSVRSFNPEL PVTTNMHMID GVDYRKMAKI LDVVSWDAYP DWGYTEDDDD ARLAAWTAMH
HDMYRSFKKK PFLLMESTPS LTNWQTVSKL KRPGMHKLSS LQAVAHGSDS VQYFQWRKSR
GSSEKFHGAV IDHSGHAETR VFRDVAEVGR TLAGLTDVVG TTTPAETAIL FDWDNRWAVK
DAQGIRNSGL KYEETVLQHY RGLWELGIPV DVVGSGDDLS GYKLVIAPML YLISEENGKR
IEKYVEQGGT FLATYWSGVV GETDLCHLGG FPGPLRRTLG IWAEETEGLH SRDLNGLVMD
PGNTLKLTGD YDAHEIAELI HLEGAEALGH YRSDFYAGRP ALTVNKLGAG KAYHLATRVK
DPQFYVDLYA AITGKERISR TLNSELPTGV TAQLRSDGEH DYVFVQNFSG NEQVVTLDGQ
EYTDLESGTA APAELKLPVN GLVMLKRPTA KA
//
