ID A0A0C7KF02_KLEPN Unreviewed; 883 AA.
AC A0A0C7KF02; A0A4V0HLW8;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 74.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595,
GN ECO:0000313|EMBL:SXU35823.1};
GN ORFNames=B5L96_08740 {ECO:0000313|EMBL:OVF74895.1}, B6I68_17890
GN {ECO:0000313|EMBL:PLE26433.1}, BB784_26890
GN {ECO:0000313|EMBL:APP55325.1}, CK244_010110
GN {ECO:0000313|EMBL:QBH43288.1}, DL426_01585
GN {ECO:0000313|EMBL:QDX69283.1}, FXN67_21555
GN {ECO:0000313|EMBL:TYL75599.1}, G4V31_22620
GN {ECO:0000313|EMBL:NGN74905.1}, GJJ13_024690
GN {ECO:0000313|EMBL:QOU78642.1}, GJJ18_19620
GN {ECO:0000313|EMBL:MRL37645.1}, HJX03_26435
GN {ECO:0000313|EMBL:QJL31824.1}, NCTC11679_05578
GN {ECO:0000313|EMBL:STV73346.1}, NCTC13443_00741
GN {ECO:0000313|EMBL:STT00482.1}, NCTC13465_05417
GN {ECO:0000313|EMBL:SQC57476.1}, NCTC5052_03709
GN {ECO:0000313|EMBL:STT95223.1}, NCTC5053_03147
GN {ECO:0000313|EMBL:STV24181.1}, NCTC9617_03512
GN {ECO:0000313|EMBL:STW46973.1}, NCTC9661_00869
GN {ECO:0000313|EMBL:STU28368.1}, QIG75_21055
GN {ECO:0000313|EMBL:MDH8291066.1}, SAMEA3499901_04187
GN {ECO:0000313|EMBL:SXN33138.1}, SAMEA3512100_04613
GN {ECO:0000313|EMBL:SXU35823.1}, SAMEA3515122_04687
GN {ECO:0000313|EMBL:SYH36520.1}, SAMEA3538828_04359
GN {ECO:0000313|EMBL:SYR46283.1}, SAMEA4364603_04516
GN {ECO:0000313|EMBL:SSK55288.1}, SAMEA4873632_04638
GN {ECO:0000313|EMBL:VGL11933.1}, VKR_04938
GN {ECO:0000313|EMBL:CAH1476644.1};
OS Klebsiella pneumoniae.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX NCBI_TaxID=573 {ECO:0000313|EMBL:SXU35823.1, ECO:0000313|Proteomes:UP000259753};
RN [1] {ECO:0000313|EMBL:APP55325.1, ECO:0000313|Proteomes:UP000184679}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Kp_Goe_827024 {ECO:0000313|EMBL:APP55325.1,
RC ECO:0000313|Proteomes:UP000184679};
RA Bohne W., Bunk B., Overmann J., Gross U., Zautner A.E.;
RT "A nosocomial outbreak of oxa48-expressing Klebsialla pneumoniae in Lower
RT Saxony.";
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:PLE26433.1, ECO:0000313|Proteomes:UP000234439}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=COL-Kpn30 {ECO:0000313|EMBL:PLE26433.1,
RC ECO:0000313|Proteomes:UP000234439};
RX PubMed=29029188; DOI=.1093/infdis/jix524;
RA Rojas L.J., Weinstock G.M., De La Cadena E., Diaz L., Rios R., Hanson B.M.,
RA Brown J.S., Vats P., Phillips D.S., Nguyen H., Hujer K.M., Correa A.,
RA Adams M.D., Perez F., Sodergren E., Narechania A., Planet P.J.,
RA Villegas M.V., Bonomo R.A., Arias C.A.;
RT "An Analysis of the Epidemic of Klebsiella pneumoniae Carbapenemase-
RT Producing K. pneumoniae: Convergence of Two Evolutionary Mechanisms Creates
RT the "Perfect Storm".";
RL J. Infect. Dis. 217:82-92(2017).
RN [3] {ECO:0000313|EMBL:OVF74895.1, ECO:0000313|Proteomes:UP000196447}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=39383 {ECO:0000313|EMBL:OVF74895.1,
RC ECO:0000313|Proteomes:UP000196447};
RA Fouts D., Stalin M.J., Chen L., Wright M., Sutton G., Nguyen K.,
RA Vanduin D., Rojas L., Hujer A., Hujer K., Bonomo R., Kreiswirth B.,
RA Adams M.;
RL Submitted (MAR-2017) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:QDX69283.1, ECO:0000313|Proteomes:UP000320592}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DA12090 {ECO:0000313|EMBL:QDX69283.1,
RC ECO:0000313|Proteomes:UP000320592};
RA Sandegren L.;
RT "Genome sequence of Klebsiella pneumoniae DA12090 (CAS55).";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|Proteomes:UP000251721, ECO:0000313|Proteomes:UP000254103}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC11679 {ECO:0000313|EMBL:STV73346.1,
RC ECO:0000313|Proteomes:UP000255239}, NCTC13443
RC {ECO:0000313|EMBL:STT00482.1, ECO:0000313|Proteomes:UP000255518},
RC NCTC13465 {ECO:0000313|EMBL:SQC57476.1,
RC ECO:0000313|Proteomes:UP000251721}, NCTC5052
RC {ECO:0000313|EMBL:STT95223.1, ECO:0000313|Proteomes:UP000254103},
RC NCTC5053 {ECO:0000313|EMBL:STV24181.1,
RC ECO:0000313|Proteomes:UP000254387}, NCTC9617
RC {ECO:0000313|EMBL:STW46973.1, ECO:0000313|Proteomes:UP000255167}, and
RC NCTC9661 {ECO:0000313|EMBL:STU28368.1,
RC ECO:0000313|Proteomes:UP000255137};
RG Pathogen Informatics;
RA Doyle S.;
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
RN [6] {ECO:0000313|Proteomes:UP000258253, ECO:0000313|Proteomes:UP000258673}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=4300STDY6470422 {ECO:0000313|EMBL:SSK55288.1,
RC ECO:0000313|Proteomes:UP000252603}, 5012STDY7626430
RC {ECO:0000313|EMBL:VGL11933.1, ECO:0000313|Proteomes:UP000376235},
RC EuSCAPE_AT029 {ECO:0000313|EMBL:SXN33138.1,
RC ECO:0000313|Proteomes:UP000259975}, EuSCAPE_DE072
RC {ECO:0000313|EMBL:SXU35823.1, ECO:0000313|Proteomes:UP000259753},
RC EuSCAPE_HU047 {ECO:0000313|EMBL:SYR46283.1,
RC ECO:0000313|Proteomes:UP000258253}, and EuSCAPE_IT093
RC {ECO:0000313|EMBL:SYH36520.1, ECO:0000313|Proteomes:UP000258673};
RG Pathogen Informatics;
RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
RN [7] {ECO:0000313|EMBL:QBH43288.1, ECO:0000313|Proteomes:UP000218354}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VBA2172 {ECO:0000313|EMBL:QBH43288.1,
RC ECO:0000313|Proteomes:UP000218354};
RA Veeraraghavan B., Shankar C., Vasudevan K., Venkatesan M., Narasimman V.;
RT "VBA2172 Klebsiella pneumoniae hybrid assembly.";
RL Submitted (FEB-2019) to the EMBL/GenBank/DDBJ databases.
RN [8] {ECO:0000313|EMBL:TYL75599.1, ECO:0000313|Proteomes:UP000322977}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UCO-494 {ECO:0000313|EMBL:TYL75599.1,
RC ECO:0000313|Proteomes:UP000322977};
RA Morales-Leon F., Caro C., Opazo-Capurro A., Lincopan N.,
RA Dominguez-Yevenes M., Lima C., Bello-Toledo H., Gonzalez-Rocha G.;
RT "Phenotypic and genetic characterization of extended-spectrum b-lactamase-
RT producing hypermucoviscous Klebsiella pneumoniae from Chile.";
RL Submitted (AUG-2019) to the EMBL/GenBank/DDBJ databases.
RN [9] {ECO:0000313|EMBL:MRL37645.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CriePir115 {ECO:0000313|EMBL:MRL37645.1};
RA Shelenkov A., Mikhaylova Y., Yanushevich Y., Samoilov A., Petrova L.,
RA Fomina V., Gusarov V., Zamyatin M., Shagin D.;
RT "Molecular typing, antibiotic resistance determination and virulence
RT profiling for 36 multidrug-resistant clinical Klebsiella pneumoniae
RT isolates using second- and third-generation sequencing.";
RL Submitted (OCT-2019) to the EMBL/GenBank/DDBJ databases.
RN [10] {ECO:0000313|EMBL:QOU78642.1, ECO:0000313|Proteomes:UP000438340}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CriePir45 {ECO:0000313|EMBL:QOU78642.1,
RC ECO:0000313|Proteomes:UP000438340};
RX PubMed=32429555;
RA Shelenkov A., Mikhaylova Y., Yanushevich Y., Samoilov A., Petrova L.,
RA Fomina V., Gusarov V., Zamyatin M., Shagin D., Akimkin V.;
RT "Molecular Typing, Characterization of Antimicrobial Resistance, Virulence
RT Profiling and Analysis of Whole-Genome Sequence of Clinical Klebsiella
RT pneumoniae Isolates.";
RL Antibiotics 9:E261-E261(2020).
RN [11] {ECO:0000313|EMBL:NGN74905.1, ECO:0000313|Proteomes:UP000479475}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2429 {ECO:0000313|EMBL:NGN74905.1,
RC ECO:0000313|Proteomes:UP000479475};
RA Starkova P.S., Sulyan O.S., Likholetova D.V., Ageevets V.A., Lazareva I.V.,
RA Sopova J.V., Sidorenko S.V.;
RT "Klebsiella pneumoniae genome sequencing and assembly.";
RL Submitted (FEB-2020) to the EMBL/GenBank/DDBJ databases.
RN [12] {ECO:0000313|EMBL:QJL31824.1, ECO:0000313|Proteomes:UP000503203}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C16KP0122 {ECO:0000313|EMBL:QJL31824.1,
RC ECO:0000313|Proteomes:UP000503203};
RA Yoon E.-J., Gwon B., Liu C., Kim D., Won D., Park S.G., Choi J.R.,
RA Jeong S.H.;
RT "Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
RT pneumoniae ensuring stable propagation of the multidrug-resistant pathogen
RT in clinical settings.";
RL Submitted (APR-2020) to the EMBL/GenBank/DDBJ databases.
RN [13] {ECO:0000313|EMBL:CAH1476644.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=BB1542 {ECO:0000313|EMBL:CAH1476644.1};
RA Delgado-Blas J.;
RL Submitted (JAN-2022) to the EMBL/GenBank/DDBJ databases.
RN [14] {ECO:0000313|EMBL:MDH8291066.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Sgh23 {ECO:0000313|EMBL:MDH8291066.1};
RA Chen Y., Gan Y.-H.;
RT "WGS of Klebsiella strains from the Antibiotics for Klebsiella Liver
RT Abscess Syndrome Study (A-KLASS) clinical trial, Singapore.";
RL Submitted (APR-2023) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC Rule:MF_00595};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946,
CC ECO:0000256|HAMAP-Rule:MF_00595};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
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DR EMBL; CP018701; APP55325.1; -; Genomic_DNA.
DR EMBL; OV753987; CAH1476644.1; -; Genomic_DNA.
DR EMBL; JARYWG010000022; MDH8291066.1; -; Genomic_DNA.
DR EMBL; WJWF01000021; MRL37645.1; -; Genomic_DNA.
DR EMBL; JAAKYD010000024; NGN74905.1; -; Genomic_DNA.
DR EMBL; NDBK01000040; OVF74895.1; -; Genomic_DNA.
DR EMBL; NCMJ01000097; PLE26433.1; -; Genomic_DNA.
DR EMBL; CP036320; QBH43288.1; -; Genomic_DNA.
DR EMBL; CP030072; QDX69283.1; -; Genomic_DNA.
DR EMBL; CP052431; QJL31824.1; -; Genomic_DNA.
DR EMBL; CP063023; QOU78642.1; -; Genomic_DNA.
DR EMBL; UAWQ01000020; SQC57476.1; -; Genomic_DNA.
DR EMBL; UFEU01000016; SSK55288.1; -; Genomic_DNA.
DR EMBL; UGKT01000001; STT00482.1; -; Genomic_DNA.
DR EMBL; UGLJ01000002; STT95223.1; -; Genomic_DNA.
DR EMBL; UGLQ01000003; STU28368.1; -; Genomic_DNA.
DR EMBL; UGMN01000004; STV24181.1; -; Genomic_DNA.
DR EMBL; UGMG01000001; STV73346.1; -; Genomic_DNA.
DR EMBL; UGNC01000005; STW46973.1; -; Genomic_DNA.
DR EMBL; UKGE01000018; SXN33138.1; -; Genomic_DNA.
DR EMBL; UKLK01000012; SXU35823.1; -; Genomic_DNA.
DR EMBL; UKUT01000014; SYH36520.1; -; Genomic_DNA.
DR EMBL; ULCI01000020; SYR46283.1; -; Genomic_DNA.
DR EMBL; VSSY01000022; TYL75599.1; -; Genomic_DNA.
DR EMBL; CAAHCC010000012; VGL11933.1; -; Genomic_DNA.
DR RefSeq; WP_004181636.1; NZ_WYAM01000027.1.
DR KEGG; kpb:FH42_17580; -.
DR KEGG; kpx:PMK1_01735; -.
DR Proteomes; UP000184679; Chromosome.
DR Proteomes; UP000196447; Unassembled WGS sequence.
DR Proteomes; UP000218354; Chromosome.
DR Proteomes; UP000234439; Unassembled WGS sequence.
DR Proteomes; UP000251721; Unassembled WGS sequence.
DR Proteomes; UP000252603; Unassembled WGS sequence.
DR Proteomes; UP000254103; Unassembled WGS sequence.
DR Proteomes; UP000254387; Unassembled WGS sequence.
DR Proteomes; UP000255137; Unassembled WGS sequence.
DR Proteomes; UP000255167; Unassembled WGS sequence.
DR Proteomes; UP000255239; Unassembled WGS sequence.
DR Proteomes; UP000255518; Unassembled WGS sequence.
DR Proteomes; UP000258253; Unassembled WGS sequence.
DR Proteomes; UP000258673; Unassembled WGS sequence.
DR Proteomes; UP000259753; Unassembled WGS sequence.
DR Proteomes; UP000259975; Unassembled WGS sequence.
DR Proteomes; UP000320592; Chromosome.
DR Proteomes; UP000322977; Unassembled WGS sequence.
DR Proteomes; UP000376235; Unassembled WGS sequence.
DR Proteomes; UP000438340; Chromosome.
DR Proteomes; UP000479475; Unassembled WGS sequence.
DR Proteomes; UP000503203; Chromosome.
DR Proteomes; UP001157099; Unassembled WGS sequence.
DR Proteomes; UP001158595; Chromosome.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR018129; PEP_COase_Lys_AS.
DR InterPro; IPR033129; PEPCASE_His_AS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR Pfam; PF00311; PEPcase; 1.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR PROSITE; PS00781; PEPCASE_1; 1.
DR PROSITE; PS00393; PEPCASE_2; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW Rule:MF_00595};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW Pyruvate {ECO:0000313|EMBL:OVF74895.1}.
FT ACT_SITE 138
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10111"
FT ACT_SITE 546
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10112"
SQ SEQUENCE 883 AA; 98946 MW; 7E3810AF3B98025F CRC64;
MNEQYSALRS NVSMLGKVLG DTIKDALGEN ILDRVETIRK LSKSSRAGNE ANRQELLTTL
QNLSNDELLP VARAFSQFLN LANTAEQYHS ISANGEAASN PEVIARTLRK LKDQPNLNEE
TIKQAVESLS LELVLTAHPT EITRRTLIHK MVEVNNCLKQ LDNKDIADYE HHQLMRRLRQ
LIAQSWHTDE IRKHRPSPVD EAKWGFAVVE NSLWEGVPNY LRELNEQLEA NLGYQLPVDF
VPVRFTSWMG GDRDGNPNVT ADITRHVLLL SRWKATDLFL KDVQVLISEL SMVECTDELR
ALAGAEGAQE PYRYLMKKLR SQLMETQAWL EARLKGQKLP KPAGLITQNE QLWEPLYACY
KSLQACGMGI IANGELLDTL RRVKSFGVPL VRIDIRQEST RHTEALGEMT RYLGIGDYES
WSEADKQAFL IRELNSKRPL LPRQWEPSEE TREVLDTCKV IAEAPRGSIA AYVISMAKTP
SDVLAVHLLL KEAGIGFALP VAPLFETLDD LNNANDVMTQ LLNIDWYRGF IQGKQMVMIG
YSDSAKDAGV MAASWAQYQA QDALIKTCEK AGIELTLFHG RGGSIGRGGA PAHAALLSQP
PGSLKGGLRV TEQGEMIRFK YGLPEVTISS LSLYTSAILE ANLLPPPEPK AEWRDIMAEL
SDVSCKMYRG YVRENKDFVP YFRSATPEQE LGKLPLGSRP AKRRPTGGVE SLRAIPWIFA
WTQNRLMLPA WLGAGAALQK VVEGGKQSEL EAMCRDWPFF STRLGMLEMV YSKADLWLAE
YYDQRLVKPE LWALGSELRK LLAADINVVL AIANDSHLMA DLPWIAESIQ LRNIYTDPLN
VLQAELLHRS RQAEEEGKDP DPRVEQALMV TIAGVAAGMR NTG
//
