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Database: UniProt/TrEMBL
Entry: A0A0H2YQR1_CLOP1
LinkDB: A0A0H2YQR1_CLOP1
Original site: A0A0H2YQR1_CLOP1 
ID   A0A0H2YQR1_CLOP1        Unreviewed;       694 AA.
AC   A0A0H2YQR1;
DT   16-SEP-2015, integrated into UniProtKB/TrEMBL.
DT   16-SEP-2015, sequence version 1.
DT   30-AUG-2017, entry version 20.
DE   SubName: Full=Sialidase {ECO:0000313|EMBL:ABG83208.1};
GN   Name=nanI {ECO:0000313|EMBL:ABG83208.1};
GN   OrderedLocusNames=CPF_0721 {ECO:0000313|EMBL:ABG83208.1};
OS   Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 /
OS   NCIMB 6125 / NCTC 8237 / Type A).
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=195103 {ECO:0000313|EMBL:ABG83208.1, ECO:0000313|Proteomes:UP000001823};
RN   [1] {ECO:0000313|EMBL:ABG83208.1, ECO:0000313|Proteomes:UP000001823}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / S
RC   107 / Type A {ECO:0000313|Proteomes:UP000001823};
RX   PubMed=16825665; DOI=10.1101/gr.5238106;
RA   Myers G.S., Rasko D.A., Cheung J.K., Ravel J., Seshadri R.,
RA   Deboy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C.,
RA   Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J.,
RA   Sullivan S.A., Khouri H., Dimitrov G.I., Watkins K.L., Mulligan S.,
RA   Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H.,
RA   Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I.,
RA   Melville S.B., Paulsen I.T.;
RT   "Skewed genomic variability in strains of the toxigenic bacterial
RT   pathogen, Clostridium perfringens.";
RL   Genome Res. 16:1031-1040(2006).
RN   [2] {ECO:0000213|PDB:5FRA, ECO:0000213|PDB:5FRE}
RP   X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 48-236 IN COMPLEX WITH
RP   CALCIUM AND GALACTOSE.
RX   PubMed=27208171; DOI=10.1042/BCJ20160340;
RA   Ribeiro J.P., Pau W., Pifferi C., Renaudet O., Varrot A., Mahal L.K.,
RA   Imberty A.;
RT   "Characterization of a high-affinity sialic acid-specific CBM40 from
RT   Clostridium perfringens and engineering of a divalent form.";
RL   Biochem. J. 473:2109-2118(2016).
RN   [3] {ECO:0000213|PDB:5TSP}
RP   X-RAY CRYSTALLOGRAPHY (1.24 ANGSTROMS) OF 243-694 IN COMPLEX WITH
RP   CALCIUM.
RX   PubMed=28315686; DOI=10.1016/j.bbrc.2017.03.064;
RA   Lee Y., Youn H.S., Lee J.G., An J.Y., Park K.R., Kang J.Y., Ryu Y.B.,
RA   Jin M.S., Park K.H., Eom S.H.;
RT   "Crystal structure of the catalytic domain of Clostridium perfringens
RT   neuraminidase in complex with a non-carbohydrate-based inhibitor, 2-
RT   (cyclohexylamino)ethanesulfonic acid.";
RL   Biochem. Biophys. Res. Commun. 486:470-475(2017).
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DR   EMBL; CP000246; ABG83208.1; -; Genomic_DNA.
DR   RefSeq; WP_011590331.1; NC_008261.1.
DR   PDB; 5FRA; X-ray; 2.00 A; A/B/C/D/E/F=48-236.
DR   PDB; 5FRE; X-ray; 1.90 A; A/B/C=48-236.
DR   PDB; 5TSP; X-ray; 1.24 A; A/B=243-694.
DR   PDBsum; 5FRA; -.
DR   PDBsum; 5FRE; -.
DR   PDBsum; 5TSP; -.
DR   SMR; A0A0H2YQR1; -.
DR   STRING; 195103.CPF_0721; -.
DR   EnsemblBacteria; ABG83208; ABG83208; CPF_0721.
DR   GeneID; 29572156; -.
DR   KEGG; cpf:CPF_0721; -.
DR   KO; K01186; -.
DR   OMA; TMHEGKE; -.
DR   Proteomes; UP000001823; Chromosome.
DR   GO; GO:0004308; F:exo-alpha-sialidase activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   InterPro; IPR013320; ConA-like_dom.
DR   InterPro; IPR004124; Glyco_hydro_33_N.
DR   InterPro; IPR026856; Sialidase_fam.
DR   InterPro; IPR011040; Sialidases.
DR   PANTHER; PTHR10628; PTHR10628; 1.
DR   Pfam; PF13088; BNR_2; 1.
DR   Pfam; PF02973; Sialidase; 1.
DR   SUPFAM; SSF49899; SSF49899; 1.
DR   SUPFAM; SSF50939; SSF50939; 2.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0000213|PDB:5FRA, ECO:0000213|PDB:5FRE,
KW   ECO:0000213|PDB:5TSP};
KW   Calcium {ECO:0000213|PDB:5FRE, ECO:0000213|PDB:5TSP};
KW   Complete proteome {ECO:0000313|Proteomes:UP000001823};
KW   Metal-binding {ECO:0000213|PDB:5FRE, ECO:0000213|PDB:5TSP}.
FT   DOMAIN       50    236       Sialidase. {ECO:0000259|Pfam:PF02973}.
FT   DOMAIN      428    615       Sialidases. {ECO:0000259|Pfam:PF13088}.
FT   METAL        87     87       Calcium 1. {ECO:0000213|PDB:5FRE}.
FT   METAL        88     88       Calcium 1; via carbonyl oxygen.
FT                                {ECO:0000213|PDB:5FRE}.
FT   METAL       149    149       Calcium 1; via carbonyl oxygen.
FT                                {ECO:0000213|PDB:5FRE}.
FT   METAL       178    178       Calcium 2. {ECO:0000213|PDB:5FRE}.
FT   METAL       180    180       Calcium 2; via carbonyl oxygen.
FT                                {ECO:0000213|PDB:5FRE}.
FT   METAL       296    296       Calcium 3. {ECO:0000213|PDB:5TSP}.
FT   METAL       296    296       Calcium 4. {ECO:0000213|PDB:5TSP}.
FT   METAL       298    298       Calcium 3. {ECO:0000213|PDB:5TSP}.
FT   METAL       319    319       Calcium 3. {ECO:0000213|PDB:5TSP}.
FT   METAL       319    319       Calcium 4. {ECO:0000213|PDB:5TSP}.
FT   METAL       320    320       Calcium 4; via carbonyl oxygen.
FT                                {ECO:0000213|PDB:5TSP}.
FT   BINDING     127    127       Galactose. {ECO:0000213|PDB:5FRA}.
SQ   SEQUENCE   694 AA;  77405 MW;  1F36DE00F026E958 CRC64;
     MNYKGITLIL TAAMVISGGN YVLVKGSTLD SGKNNSGYEI KVNNSENLSS LGEYKDINLE
     SSNASNITYD LEKYKNLDEG TIVVRFNSKD SKIQSLLGIS NSKTKNGYFN FYVTNSRVGF
     ELRNQKNEGN TQNGTENLVH MYKDVALNDG DNTVALKIEK NKGYKLFLNG KMIKEVKDTN
     TKFLNNIENL DSAFIGKTNR YGQSNEYNFK GNIGFMNIYN EPLGDDYLLS KTGETKAKEE
     VLVEGAVKTE PVDLFHPGFL NSSNYRIPAL FKTKEGTLIA SIDARRQGGA DAPNNDIDTA
     VRRSEDGGKT WDEGQIIMDY PDKSSVIDTT LIQDDETGRI FLLVTHFPSK YGFWNAGLGS
     GFKNIDGKEY LCLYDSSGKE FTVRENVVYD KDGNKTEYTT NALGDLFRNG TKIDNINSST
     APLKAKGTSY INLVYSDDDG KTWSEPQNIN FQVKKDWMKF LGIAPGRGIQ IKNGEHKGRI
     VVPVYYTNEK GKQSSAVIYS DDSGKNWTIG ESPNDNRKLE NGKIINSKTL SDDAPQLTEC
     QVVEMPNGQL KLFMRNLSGY LNIATSFDGG ATWDETVEKD TNVLEPYCQL SVINYSQKID
     GKDAVIFSNP NARSRSNGTV RIGLINQVGT YENGEPKYEF DWKYNKLVKP GYYAYSCLTE
     LSNGNIGLLY EGTPSEEMSY IEMNLKYLES GANK
//
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