ID A0A0U2M7H3_9BACL Unreviewed; 458 AA.
AC A0A0U2M7H3;
DT 16-MAR-2016, integrated into UniProtKB/TrEMBL.
DT 16-MAR-2016, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=Glutamate dehydrogenase {ECO:0000256|ARBA:ARBA00012896, ECO:0000256|PIRNR:PIRNR000185};
GN ORFNames=IJ22_37740 {ECO:0000313|EMBL:ALS24112.1}, PN4B1_22350
GN {ECO:0000313|EMBL:GCL72330.1}, SAMN05421868_12045
GN {ECO:0000313|EMBL:SDJ20508.1};
OS Paenibacillus naphthalenovorans.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=162209 {ECO:0000313|EMBL:ALS24112.1, ECO:0000313|Proteomes:UP000061660};
RN [1] {ECO:0000313|Proteomes:UP000061660}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=32O-Y {ECO:0000313|Proteomes:UP000061660};
RA Butler R.III., Wang J., Stark B.C., Pombert J.-F.;
RT "Complete genome sequences of two moderately thermophilic Paenibacillus
RT species.";
RL Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ALS24112.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=32O-Y {ECO:0000313|EMBL:ALS24112.1};
RA Shamseldin A., Moawad H., Abd El-Rahim W.M., Sadowsky M.J.;
RL Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:ALS24112.1, ECO:0000313|Proteomes:UP000061660}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=32O-Y {ECO:0000313|EMBL:ALS24112.1,
RC ECO:0000313|Proteomes:UP000061660};
RX PubMed=26868401;
RA Butler R.R.III., Wang J., Stark B.C., Pombert J.F.;
RT "Complete Genome Sequences of Two Interactive Moderate Thermophiles,
RT Paenibacillus napthalenovorans 32O-Y and Paenibacillus sp. 32O-W.";
RL Genome Announc. 4:e01717-15(2016).
RN [4] {ECO:0000313|EMBL:SDJ20508.1, ECO:0000313|Proteomes:UP000182184}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PR-N1 {ECO:0000313|EMBL:SDJ20508.1,
RC ECO:0000313|Proteomes:UP000182184};
RA Varghese N., Submissions S.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:GCL72330.1, ECO:0000313|Proteomes:UP000299945}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=4B1 {ECO:0000313|EMBL:GCL72330.1,
RC ECO:0000313|Proteomes:UP000299945};
RA Ha-Thanh L.T., Ngoc-Thi T.V., Shintani M., Moriuchi R., Dohra H.,
RA Hoang-Loc N., Kimbara K.;
RT "Isolation and characterization of a moderate thermophilic Paenibacillus
RT naphthalenovorans strain 4B1 capable of degrading dibenzofuran from dioxin-
RT contaminated soil in Vietnam.";
RL Submitted (MAR-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBUNIT: Homohexamer. {ECO:0000256|ARBA:ARBA00011643}.
CC -!- SIMILARITY: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
CC {ECO:0000256|ARBA:ARBA00006382, ECO:0000256|PIRNR:PIRNR000185,
CC ECO:0000256|RuleBase:RU004417}.
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DR EMBL; CP013652; ALS24112.1; -; Genomic_DNA.
DR EMBL; BJCS01000005; GCL72330.1; -; Genomic_DNA.
DR EMBL; FNDY01000020; SDJ20508.1; -; Genomic_DNA.
DR RefSeq; WP_054818182.1; NZ_FNDY01000020.1.
DR STRING; 162209.IJ22_37740; -.
DR KEGG; pnp:IJ22_37740; -.
DR PATRIC; fig|162209.4.peg.4020; -.
DR OrthoDB; 9803297at2; -.
DR Proteomes; UP000061660; Chromosome.
DR Proteomes; UP000182184; Unassembled WGS sequence.
DR Proteomes; UP000299945; Unassembled WGS sequence.
DR GO; GO:0004352; F:glutamate dehydrogenase (NAD+) activity; IEA:UniProtKB-EC.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0006520; P:amino acid metabolic process; IEA:InterPro.
DR CDD; cd05313; NAD_bind_2_Glu_DH; 1.
DR Gene3D; 1.10.285.10; Glutamate Dehydrogenase, chain A, domain 3; 2.
DR Gene3D; 3.40.50.10860; Leucine Dehydrogenase, chain A, domain 1; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR046346; Aminoacid_DH-like_N_sf.
DR InterPro; IPR006095; Glu/Leu/Phe/Val/Trp_DH.
DR InterPro; IPR006096; Glu/Leu/Phe/Val/Trp_DH_C.
DR InterPro; IPR006097; Glu/Leu/Phe/Val/Trp_DH_dimer.
DR InterPro; IPR014362; Glu_DH.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR033922; NAD_bind_Glu_DH.
DR PANTHER; PTHR43571; NADP-SPECIFIC GLUTAMATE DEHYDROGENASE 1-RELATED; 1.
DR PANTHER; PTHR43571:SF1; NADP-SPECIFIC GLUTAMATE DEHYDROGENASE 1-RELATED; 1.
DR Pfam; PF00208; ELFV_dehydrog; 1.
DR Pfam; PF02812; ELFV_dehydrog_N; 1.
DR PIRSF; PIRSF000185; Glu_DH; 1.
DR PRINTS; PR00082; GLFDHDRGNASE.
DR SMART; SM00839; ELFV_dehydrog; 1.
DR SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW NAD {ECO:0000256|PIRSR:PIRSR000185-2};
KW Nucleotide-binding {ECO:0000256|PIRSR:PIRSR000185-2};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|PIRNR:PIRNR000185};
KW Reference proteome {ECO:0000313|Proteomes:UP000061660}.
FT DOMAIN 215..456
FT /note="Glutamate/phenylalanine/leucine/valine/L-tryptophan
FT dehydrogenase C-terminal"
FT /evidence="ECO:0000259|SMART:SM00839"
FT ACT_SITE 138
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000185-1"
FT BINDING 102
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000185-2"
FT BINDING 123
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000185-2"
FT BINDING 126
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000185-2"
FT BINDING 177
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000185-2"
FT BINDING 222
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000256|PIRSR:PIRSR000185-2"
FT BINDING 253
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000256|PIRSR:PIRSR000185-2"
FT BINDING 390
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000185-2"
FT SITE 178
FT /note="Important for catalysis"
FT /evidence="ECO:0000256|PIRSR:PIRSR000185-3"
SQ SEQUENCE 458 AA; 49856 MW; 4C0E7BD3FA89E9E5 CRC64;
MALETAQHNE LAARTYVNEV YDTVIKRNPH ESEFHQAIKE ILFSLVPVFA KHPKYMQAGI
LERIVEPERM ITFRVPWVDD QGKVQVNRGF RVQFNSVLGP YKGGLRFHPS VNASIIKFLG
FEQIFKNSLT GQPIGGGKGG SDFDPKGKSD GEIMRFTQSF MTELFKYIGP DTDVPAGDIG
VGAREIGYMF GQYRRIRGGN EAGVLTGKGI GYGGSLTRTE ATGYGTVYFV EEMLKSIGLS
FEDSTVVISG SGNVSLYAIQ KATQLGAKVL ACSDSNGYVY DPDGIKFETV KQIKEVERKR
ISEYVKEHPN AEYHEGCEGI WTIPCDIALP CATQNEISEA SAKLLVANGV KAVGEGANMP
STLEAIDVFL ANKVLFGPAK AANAGGVAVS ALEMAQNSMR YSWSFHEVDD KLHAIMKNIY
RSSVQAAEEY GQPGNLLVGA NIAGFTKVAD AMLAQGII
//
