GenomeNet

Database: UniProt/TrEMBL
Entry: A0A1B1BN76_9MICO
LinkDB: A0A1B1BN76_9MICO
Original site: A0A1B1BN76_9MICO 
ID   A0A1B1BN76_9MICO        Unreviewed;       404 AA.
AC   A0A1B1BN76;
DT   02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT   02-NOV-2016, sequence version 1.
DT   07-JUN-2017, entry version 6.
DE   RecName: Full=Isocitrate dehydrogenase [NADP] {ECO:0000256|PIRNR:PIRNR000108};
DE            EC=1.1.1.42 {ECO:0000256|PIRNR:PIRNR000108};
GN   ORFNames=PA27867_2865 {ECO:0000313|EMBL:ANP73803.1};
OS   Cryobacterium arcticum.
OC   Bacteria; Actinobacteria; Micrococcales; Microbacteriaceae;
OC   Cryobacterium.
OX   NCBI_TaxID=670052 {ECO:0000313|EMBL:ANP73803.1, ECO:0000313|Proteomes:UP000092582};
RN   [1] {ECO:0000313|EMBL:ANP73803.1, ECO:0000313|Proteomes:UP000092582}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PAMC 27867 {ECO:0000313|EMBL:ANP73803.1,
RC   ECO:0000313|Proteomes:UP000092582};
RA   Lee J., Kim O.-S.;
RT   "Genome sequencing of Cryobacterium arcticum PAMC 27867.";
RL   Submitted (JUN-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: Isocitrate + NADP(+) = 2-oxoglutarate + CO(2)
CC       + NADPH. {ECO:0000256|PIRNR:PIRNR000108}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|PIRNR:PIRNR000108,
CC         ECO:0000256|PIRSR:PIRSR000108-3};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000256|PIRNR:PIRNR000108,
CC         ECO:0000256|PIRSR:PIRSR000108-3};
CC       Note=Binds 1 Mg(2+) or Mn(2+) ion per subunit.
CC       {ECO:0000256|PIRNR:PIRNR000108, ECO:0000256|PIRSR:PIRSR000108-3};
CC   -!- SIMILARITY: Belongs to the isocitrate and isopropylmalate
CC       dehydrogenases family. {ECO:0000256|PIRNR:PIRNR000108}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; CP016282; ANP73803.1; -; Genomic_DNA.
DR   RefSeq; WP_066597465.1; NZ_CP016282.1.
DR   EnsemblBacteria; ANP73803; ANP73803; PA27867_2865.
DR   KEGG; cart:PA27867_2865; -.
DR   PATRIC; fig|670052.7.peg.2943; -.
DR   KO; K00031; -.
DR   Proteomes; UP000092582; Chromosome 1.
DR   GO; GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; IEA:UniProtKB-EC.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0006102; P:isocitrate metabolic process; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR019818; IsoCit/isopropylmalate_DH_CS.
DR   InterPro; IPR004790; Isocitrate_DH_NADP.
DR   InterPro; IPR024084; IsoPropMal-DH-like_dom.
DR   PANTHER; PTHR11822; PTHR11822; 1.
DR   Pfam; PF00180; Iso_dh; 1.
DR   PIRSF; PIRSF000108; IDH_NADP; 1.
DR   SMART; SM01329; Iso_dh; 1.
DR   TIGRFAMs; TIGR00127; nadp_idh_euk; 1.
DR   PROSITE; PS00470; IDH_IMDH; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000092582};
KW   Magnesium {ECO:0000256|PIRNR:PIRNR000108,
KW   ECO:0000256|PIRSR:PIRSR000108-3};
KW   Manganese {ECO:0000256|PIRNR:PIRNR000108,
KW   ECO:0000256|PIRSR:PIRSR000108-3};
KW   Metal-binding {ECO:0000256|PIRNR:PIRNR000108,
KW   ECO:0000256|PIRSR:PIRSR000108-3};
KW   NADP {ECO:0000256|PIRNR:PIRNR000108, ECO:0000256|PIRSR:PIRSR000108-4};
KW   Oxidoreductase {ECO:0000256|PIRNR:PIRNR000108};
KW   Reference proteome {ECO:0000313|Proteomes:UP000092582};
KW   Tricarboxylic acid cycle {ECO:0000256|PIRNR:PIRNR000108}.
FT   DOMAIN        9    395       Iso_dh. {ECO:0000259|SMART:SM01329}.
FT   NP_BIND      75     77       NADP. {ECO:0000256|PIRSR:PIRSR000108-4}.
FT   NP_BIND     309    314       NADP. {ECO:0000256|PIRSR:PIRSR000108-4}.
FT   REGION       94    100       Substrate binding. {ECO:0000256|PIRSR:
FT                                PIRSR000108-2}.
FT   METAL       251    251       Magnesium or manganese.
FT                                {ECO:0000256|PIRSR:PIRSR000108-3}.
FT   METAL       274    274       Magnesium or manganese.
FT                                {ECO:0000256|PIRSR:PIRSR000108-3}.
FT   BINDING      77     77       Substrate. {ECO:0000256|PIRSR:
FT                                PIRSR000108-2}.
FT   BINDING      82     82       NADP. {ECO:0000256|PIRSR:PIRSR000108-4}.
FT   BINDING     109    109       Substrate. {ECO:0000256|PIRSR:
FT                                PIRSR000108-2}.
FT   BINDING     132    132       Substrate. {ECO:0000256|PIRSR:
FT                                PIRSR000108-2}.
FT   BINDING     259    259       NADP. {ECO:0000256|PIRSR:PIRSR000108-4}.
FT   BINDING     327    327       NADP; via amide nitrogen and carbonyl
FT                                oxygen. {ECO:0000256|PIRSR:PIRSR000108-
FT                                4}.
FT   SITE        139    139       Critical for catalysis.
FT                                {ECO:0000256|PIRSR:PIRSR000108-1}.
FT   SITE        211    211       Critical for catalysis.
FT                                {ECO:0000256|PIRSR:PIRSR000108-1}.
SQ   SEQUENCE   404 AA;  44905 MW;  AF796F3F7F7371FF CRC64;
     MSKIKVAGTV VELDGDEMTR IIWQAIKDSL IHPYLDIDLE YYDLSIQKRD ETDDQITIDA
     AHAIQKHGVG VKCATITPDE ARVEEFGLKK MWASPNGTIR NILGGTIFRE PIIISNIPRL
     VPGWNKPIII GRHAFGDQYR ATNFRFEGEG TLTMTFTPKD GGDPQQFEVF QAPGSGVAMG
     MYNLDKSIID FARASLNYGL ERNYPVYLST KNTILKAYDG RFKDIFQEIF DTEFKDRFEA
     AGLTYEHRLI DDMVASAMKW EGGYVWACKN YDGDVQSDTV AQGFGSLGLM TSVLSTPDGS
     VVEAEAAHGT VTRHYRQHQQ GKPTSTNPIA SIYAWTRGLS HRAKLDSNPA LAEFAHTLED
     VVVKTVESGK MTKDLALLVG PDQGYLTTEE FLAAISDNLQ TRLA
//
DBGET integrated database retrieval system