ID A0A1L7I1A0_9FLAO Unreviewed; 483 AA.
AC A0A1L7I1A0;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE SubName: Full=Cytoplasmic alpha-amylase {ECO:0000313|EMBL:APU66925.1};
DE EC=3.2.1.1 {ECO:0000313|EMBL:APU66925.1};
GN ORFNames=C723_3113 {ECO:0000313|EMBL:OSS38024.1}, GRFL_0201
GN {ECO:0000313|EMBL:APU66925.1};
OS Christiangramia flava JLT2011.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Christiangramia.
OX NCBI_TaxID=1229726 {ECO:0000313|EMBL:APU66925.1, ECO:0000313|Proteomes:UP000186230};
RN [1] {ECO:0000313|EMBL:OSS38024.1, ECO:0000313|Proteomes:UP000194120}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JLT2011 {ECO:0000313|EMBL:OSS38024.1,
RC ECO:0000313|Proteomes:UP000194120};
RX PubMed=24048873; DOI=10.1099/ijs.0.051987-0;
RA Liu K., Li S., Jiao N., Tang K.;
RT "Gramella flava sp. nov., a member of the family Flavobacteriaceae isolated
RT from seawater.";
RL Int. J. Syst. Evol. Microbiol. 64:165-168(2014).
RN [2] {ECO:0000313|EMBL:APU66925.1, ECO:0000313|Proteomes:UP000186230}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JLT2011 {ECO:0000313|EMBL:APU66925.1,
RC ECO:0000313|Proteomes:UP000186230};
RA Tang K.;
RT "Multi-omics approach to identify versatile polysaccharide utilization
RT systems of a marine flavobacterium Gramella flava.";
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP016359; APU66925.1; -; Genomic_DNA.
DR EMBL; AMRU01000014; OSS38024.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1L7I1A0; -.
DR STRING; 1229726.GRFL_0201; -.
DR KEGG; gfl:GRFL_0201; -.
DR OrthoDB; 9806009at2; -.
DR Proteomes; UP000186230; Chromosome.
DR Proteomes; UP000194120; Unassembled WGS sequence.
DR GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-EC.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11314; AmyAc_arch_bac_plant_AmyA; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013776; A-amylase_thermo.
DR InterPro; IPR015237; Alpha-amylase_C_pro.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR Pfam; PF09154; Alpha-amy_C_pro; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR PIRSF; PIRSF001021; Alph-amls_thrmst; 2.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 4: Predicted;
KW Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW Glycosidase {ECO:0000313|EMBL:APU66925.1};
KW Hydrolase {ECO:0000313|EMBL:APU66925.1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000186230};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..19
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 20..483
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5012996013"
FT DOMAIN 60..393
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT REGION 24..49
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 253
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT ACT_SITE 277
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT BINDING 160
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 220
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 228
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 234
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
SQ SEQUENCE 483 AA; 55326 MW; C99250764D8045EF CRC64;
MINKRIYFIF LLLVGVLLAS CSKDSDEPTT DPTDDQGTEQ PTPEPDPEAI DLSAYDNGER
VMMQAFYWDV EPRFEWWTNL TGKVAGWSDA GINRIWIPVA TKGQSGGYSM GYDVSDYFDF
GEFSQHGTIE TRFGSRAELE ELIQTAHDND VEVIEDIVIN HNSGGGLEYN PYRDKDTYTL
FDEEHGNASG MFNRNYEDFY PNSVSNYDPG SLFYAETNLD HNRERVQDWL WKKDNSVAKY
YQNVMGFDGW RFDYVLGYEP WVVKAWLDEV GGFSVVELWD GNADVLKNYV EETGSRAFDF
ASFYKLEEGL DRYEDLNSLT GSQLWQTYPD KSVTFTANHD TEKDSNEDNY IKRENKMKAY
AYIMTHPGYP CVFYLDYEDS EFQDELKNLI AIHNSLATGE TEVLYVDEDE YIMKRNGTGT
NPGLILYISI SNSTKRRAVS SNWNNITLMD YSGNSTYNPT SDENGAVTIE APANGYSIWS
ITE
//
