ID A1KSZ0_NEIMF Unreviewed; 787 AA.
AC A1KSZ0;
DT 06-FEB-2007, integrated into UniProtKB/TrEMBL.
DT 06-FEB-2007, sequence version 1.
DT 01-MAY-2013, entry version 53.
DE RecName: Full=Phenylalanine--tRNA ligase beta subunit;
DE EC=6.1.1.20;
DE AltName: Full=Phenylalanyl-tRNA synthetase beta subunit;
GN Name=pheT; OrderedLocusNames=NMC0681;
OS Neisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 /
OS FAM18).
OC Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales;
OC Neisseriaceae; Neisseria.
OX NCBI_TaxID=272831;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700532 / FAM18;
RX PubMed=17305430; DOI=10.1371/journal.pgen.0030023;
RA Bentley S.D., Vernikos G.S., Snyder L.A.S., Churcher C.,
RA Arrowsmith C., Chillingworth T., Cronin A., Davis P.H., Holroyd N.E.,
RA Jagels K., Maddison M., Moule S., Rabbinowitsch E., Sharp S.,
RA Unwin L., Whitehead S., Quail M.A., Achtman M., Barrell B.G.,
RA Saunders N.J., Parkhill J.;
RT "Meningococcal genetic variation mechanisms viewed through comparative
RT analysis of serogroup C strain FAM18.";
RL PLoS Genet. 3:230-240(2007).
CC -!- CATALYTIC ACTIVITY: ATP + L-phenylalanine + tRNA(Phe) = AMP +
CC diphosphate + L-phenylalanyl-tRNA(Phe).
CC -!- COFACTOR: Binds 2 magnesium ions per tetramer (By similarity).
CC -!- SUBUNIT: Tetramer of two alpha and two beta subunits (By
CC similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Belongs to the phenylalanyl-tRNA synthetase beta
CC subunit family. Type 1 subfamily.
CC -!- SIMILARITY: Contains 1 B5 domain.
CC -!- SIMILARITY: Contains 1 FDX-ACB domain.
CC -!- SIMILARITY: Contains 1 tRNA-binding domain.
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DR EMBL; AM421808; CAM09971.1; -; Genomic_DNA.
DR RefSeq; YP_974770.1; NC_008767.1.
DR ProteinModelPortal; A1KSZ0; -.
DR STRING; 272831.NMC0681; -.
DR EnsemblBacteria; CAM09971; CAM09971; NMC0681.
DR GeneID; 4675727; -.
DR KEGG; nmc:NMC0681; -.
DR PATRIC; 20351151; VBINeiMen17609_0830.
DR eggNOG; COG0073; -.
DR HOGENOM; HOG000292085; -.
DR KO; K01890; -.
DR OMA; MKFSEQW; -.
DR ProtClustDB; PRK00629; -.
DR BioCyc; NMEN272831:GJDX-665-MONOMER; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0000287; F:magnesium ion binding; IEA:HAMAP.
DR GO; GO:0004826; F:phenylalanine-tRNA ligase activity; IEA:HAMAP.
DR GO; GO:0000049; F:tRNA binding; IEA:HAMAP.
DR GO; GO:0006432; P:phenylalanyl-tRNA aminoacylation; IEA:HAMAP.
DR GO; GO:0008033; P:tRNA processing; IEA:InterPro.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.56.20; -; 1.
DR Gene3D; 3.30.70.380; -; 1.
DR Gene3D; 3.50.40.10; -; 1.
DR HAMAP; MF_00283; Phe_tRNA_synth_beta1; 1; -.
DR InterPro; IPR005146; B3/B4_tRNA-bd.
DR InterPro; IPR009061; DNA-bd_dom_put.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004532; Phe-tRNA-ligase_IIc_bsu_bac.
DR InterPro; IPR020825; Phe-tRNA_synthase_B3/B4.
DR InterPro; IPR005121; PheS_beta_Fdx_antiC-bd.
DR InterPro; IPR002547; tRNA-bd_dom.
DR InterPro; IPR005147; tRNA_synthase_B5-dom.
DR Pfam; PF03483; B3_4; 1.
DR Pfam; PF03484; B5; 1.
DR Pfam; PF03147; FDX-ACB; 1.
DR Pfam; PF01588; tRNA_bind; 1.
DR SMART; SM00873; B3_4; 1.
DR SMART; SM00874; B5; 1.
DR SMART; SM00896; FDX-ACB; 1.
DR SUPFAM; SSF56037; B3_4; 1.
DR SUPFAM; SSF54991; Fdx_AntiC_bd; 1.
DR SUPFAM; SSF50249; Nucleic_acid_OB; 1.
DR SUPFAM; SSF46955; Putativ_DNA_bind; 1.
DR TIGRFAMs; TIGR00472; pheT_bact; 1.
DR PROSITE; PS51483; B5; 1.
DR PROSITE; PS51447; FDX_ACB; 1.
DR PROSITE; PS50886; TRBD; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm;
KW Ligase; Magnesium; Metal-binding; Nucleotide-binding;
KW Protein biosynthesis; RNA-binding; tRNA-binding.
FT DOMAIN 39 149 tRNA-binding (By similarity).
FT DOMAIN 400 475 B5 (By similarity).
FT DOMAIN 694 786 FDX-ACB (By similarity).
FT METAL 453 453 Magnesium (By similarity).
FT METAL 459 459 Magnesium; via carbonyl oxygen (By
FT similarity).
FT METAL 462 462 Magnesium (By similarity).
FT METAL 463 463 Magnesium (By similarity).
SQ SEQUENCE 787 AA; 86038 MW; 0F5E0847C696C4D0 CRC64;
MQFSYSWLKT QADTELSADK LEHLLTMSGL EVEEAETAAP AFAGVVVAEV KSVEKHPDAD
RLNVTRVDAG TGELVQIVCG APNVKAGIKV PCSLPGAVLP GNFKIKPTKM RGVPSNGMLC
STNELGLPDD GVNGLHILPE DAPVGINIRE YLDLDDTLFT LKITPNRADC LSVKGIAREV
SALTGCAFRQ PEIHTAPITG SRKQPVQINA PADCGRFISR VIENVNAKAA TPDWMKQRLE
RSGIRSISVL VDIGNYVMLE IGQPMHVFDA DKLSGSLHIR RAREGETLEC LNEKTVSLSE
NTLVVADEKG ALSLAGLMGG AASAVSDGTQ NIVLEAAWFA PEIIAGKSRQ YGFGSDSSFR
FERGVDYRLQ ADAIERATEL VLQICGGAAG EMVEAQGELP EAKQVGLRLD RLKTVLGVDI
PAEQVETILQ HLGLQPEKTA EGFRVTAPSF RFDIEIEADL IEEIGRVYGY ENIPDDYTSG
RLKMLELPET RRSRFAVYNE MAARGYREVV SYAFVDEQWE QDFAANADPI RLQNPLAAQY
AVMRSTLIGG LVEILQNNLN RKQNRVRVFE IARVFGKGSD GRFVQNERIG GLWYGAAMPE
QWGGKTRNAD FYDIKADVEN LLKNKAVEFV KTGYPALHPG RAANIVSDGK VIGFVGELHP
KWLQKYDLPQ APLVFEIDMA AVLECGKTRY RAVSKFQPVR RDLAFVMPEA MSHDDLLLVL
KGAANKLVQE ISVFDVYRGM GLPEGMKSVA VKVILQDMEN TLTDEAVEPL IGKLIGAATA
AGARLRS
//
