ID A3LR63_PICST Unreviewed; 688 AA.
AC A3LR63;
DT 03-APR-2007, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2007, sequence version 2.
DT 27-MAR-2024, entry version 87.
DE RecName: Full=UDP-glucose 4-epimerase {ECO:0000256|ARBA:ARBA00013189};
DE EC=5.1.3.2 {ECO:0000256|ARBA:ARBA00013189};
GN Name=GAL10 {ECO:0000313|EMBL:ABN65328.2};
GN ORFNames=PICST_76750 {ECO:0000313|EMBL:ABN65328.2};
OS Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL
OS Y-11545) (Yeast) (Pichia stipitis).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Scheffersomyces.
OX NCBI_TaxID=322104 {ECO:0000313|EMBL:ABN65328.2, ECO:0000313|Proteomes:UP000002258};
RN [1] {ECO:0000313|EMBL:ABN65328.2, ECO:0000313|Proteomes:UP000002258}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545
RC {ECO:0000313|Proteomes:UP000002258};
RX PubMed=17334359; DOI=10.1038/nbt1290;
RA Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.S.,
RA Passoth V., Richardson P.M.;
RT "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting
RT yeast Pichia stipitis.";
RL Nat. Biotechnol. 25:319-326(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=UDP-alpha-D-glucose = UDP-alpha-D-galactose;
CC Xref=Rhea:RHEA:22168, ChEBI:CHEBI:58885, ChEBI:CHEBI:66914;
CC EC=5.1.3.2; Evidence={ECO:0000256|ARBA:ARBA00000083};
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC Evidence={ECO:0000256|ARBA:ARBA00001911};
CC -!- PATHWAY: Carbohydrate metabolism; galactose metabolism.
CC {ECO:0000256|ARBA:ARBA00004947}.
CC -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC family. {ECO:0000256|ARBA:ARBA00007637}.
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DR EMBL; CP000497; ABN65328.2; -; Genomic_DNA.
DR RefSeq; XP_001383357.2; XM_001383320.1.
DR AlphaFoldDB; A3LR63; -.
DR STRING; 322104.A3LR63; -.
DR GeneID; 4838281; -.
DR KEGG; pic:PICST_76750; -.
DR eggNOG; KOG1371; Eukaryota.
DR HOGENOM; CLU_007383_22_1_1; -.
DR InParanoid; A3LR63; -.
DR OMA; KGLYREW; -.
DR OrthoDB; 5489993at2759; -.
DR Proteomes; UP000002258; Chromosome 3.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0003978; F:UDP-glucose 4-epimerase activity; IEA:InterPro.
DR GO; GO:0006012; P:galactose metabolic process; IEA:UniProtKB-KW.
DR CDD; cd09019; galactose_mutarotase_like; 1.
DR CDD; cd05247; UDP_G4E_1_SDR_e; 1.
DR Gene3D; 2.70.98.10; -; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 3.90.25.10; UDP-galactose 4-epimerase, domain 1; 1.
DR InterPro; IPR018052; Ald1_epimerase_CS.
DR InterPro; IPR008183; Aldose_1/G6P_1-epimerase.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR047215; Galactose_mutarotase-like.
DR InterPro; IPR014718; GH-type_carb-bd.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR005886; UDP_G4E.
DR NCBIfam; TIGR01179; galE; 1.
DR PANTHER; PTHR43725; UDP-GLUCOSE 4-EPIMERASE; 1.
DR PANTHER; PTHR43725:SF51; UDP-GLUCOSE 4-EPIMERASE; 1.
DR Pfam; PF01263; Aldose_epim; 1.
DR Pfam; PF16363; GDP_Man_Dehyd; 1.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR PROSITE; PS00545; ALDOSE_1_EPIMERASE; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Reference proteome {ECO:0000313|Proteomes:UP000002258}.
FT DOMAIN 9..337
FT /note="NAD(P)-binding"
FT /evidence="ECO:0000259|Pfam:PF16363"
SQ SEQUENCE 688 AA; 76541 MW; 8C6A77E386283FD4 CRC64;
MSAPQDYILI TGGAGYIGSH TVIELVNNGY KVVVVDSLVN SSYDAIARIE YIVKQPVPFY
QVDIRDGEKL NEVFQKHKIT GVIHFAALKA VGESTEIPLE YYDNNVNGTI SLLQVMNKNN
VKSIVFSSSA TVYGDVTRFG DNSMIPIPEH CPTDPTNPYG RTKLVIENIL RDVHSSSSSE
WKVAVLRYFN PIGAHPSGLI GEDPLGIPNN LLPYLAQVAI GRREKLSVFG SDYASHDGTP
IRDYIHVVDL AKGHLAALSF LNKTNEGSFR EWNLGTGKGS TVFDVYHAFS KAVGRDLPYE
VVGRRAGDVL DLTANPTRAN KELEWKAELT IEDACKDLWK WTTGNPTGFK IEGYEWKLFE
TEGHANRLHT FTTGDVTIAL ANRGALIQDL SFQGEHLVCA YDNGDDYKLP TNPFFGTNVG
RVANRIANGE FKLNGQTYKT PKNENNNTLH GGLVGFDKRN WLGPVISKKE DVFTVKFALI
DEDGHNGFPG DLQTIITYTI EPTAVSHEYE AKLINDKVDA TIVNLTNHSY FNLSGEPTTD
DTDVTLFYDK YMEVDDKTLL PTGNLIKYPH ATANVPVHLD QDAFFDYCFI VDEDGCGIDT
RKSQLKPIFK ATHPRANTEF LFSSTEPSFQ FYTGDGVNTK GFVKRSGFCI EPARFVDSIS
FEKWAPQVIL KKGETYGSLT KYSFSKKQ
//
